CN101745002B - Extraction method of ophiopogon root, ginseng and shiandra and preparation thereof - Google Patents
Extraction method of ophiopogon root, ginseng and shiandra and preparation thereof Download PDFInfo
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- CN101745002B CN101745002B CN 200810153783 CN200810153783A CN101745002B CN 101745002 B CN101745002 B CN 101745002B CN 200810153783 CN200810153783 CN 200810153783 CN 200810153783 A CN200810153783 A CN 200810153783A CN 101745002 B CN101745002 B CN 101745002B
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- fructus schisandrae
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Abstract
The invention relates to a Chinese traditional medicine extraction method and a pharmaceutical preparation thereof, in particular to a preparation for treating cardiovascular diseases, tonifying qi and calming pulse and a preparation method thereof. The preparation is prepared from ginseng, ophiopogon root and shiandra.
Description
Technical field:
The present invention relates to a kind of Chinese medicine extraction method and pharmaceutical preparation thereof, particularly a kind of qi-tonifying and pulse-restoring preparation of treating cardiovascular disease and preparation method thereof, said preparation are by Radix Ginseng, and Radix Ophiopogonis and Fructus Schisandrae Chinensis three flavor Chinese medicines are prepared from.
Background technology:
Radix Ginseng is the root of Araliaceae Radix Ginseng Panax ginseng C.A.Mey..Sweet, the little hardship of its property of medicine, flat, return lung, spleen, heart channel; Its effect strongly invigorating primordial QI, invigorating the spleen to benefit the lung promotes the production of body fluid, the Fructus Alpiniae Oxyphyllae of calming the nerves.Use: the vigour collapse has been demonstrate,proved, lung spleen heart syndrome of deficiency of kidney-QI, and calentura deficiency of vital energy Tianjin wound reaches diabete yearningly.Its chemical constituent comprises multiple ginsenoside, volatile oil, aminoacid, trace element and organic acid, saccharide, vitamin etc.
Have another name called dwarf lilyturf, book band grass Radix Ophiopogonis, be Liliaceae Ophiopogon perennial evergreen herbaceous plant.Fibrous root is more sturdy, and often expand at the top of root or middle part becomes spindle shape meat fritter.Its nature and flavor with return through: sweet, little hardship is slightly cold GUIXIN, lung, stomach warp.Its function with cure mainly: YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire, and is used for dryness of the lung dry cough, chronic consumptive disease cough, Tianjin wound is thirsty, vexed insomnia, interior-heat is quenched one's thirst, dryness of the intestine constipation, pharyngeal diphtheria.Its chemical constituent comprises Radix Ophiopogonis polysaccharide and Radix Ophiopogonis total saponins etc.
Schisandra, commonly known as mountain pepper, sub-weights, medicine Schisandra, face rattan, five plums and other ancient medical books call it Chi Chu, mysterious and will and.It is the fruit of magnoliaceae schisandra.Fruit of Fructus Schisandrae Chinensis is made Chinese medicine function supplementing QI for promoting the production of body fluid, the nourishing kidney of astringing the lung, antidiarrheal, arresting seminal emission, is calmed the nerves, and can control diseases such as chronic cough dyspnea due to deficiency, the few xerostomia in Tianjin, seminal emission chronic diarrhea, forgetful insomnia.The pharmacological testing proof can be regulated central nervous system's excitement and process of inhibition, promotes body metabolism, regulates the secretion of gastric juice and bile, and hepatitis convalescent period transaminase rising person is had the reduction effect.Peel and the ripe skin of planting contain lignanoid, are the effective medicinal components of Fructus Schisandrae Chinensis, comprising multiple schizandrin.Seed is fatty, but oils and fats soap system or machine oil.Stem and leaf and seed all can extract aromatic oil.
Qi-tonifying and pulse-restoring preparation derives from the ancient prescription SHENGMAI SAN; SHENGMAI SAN source " medicine origin ", it is by Radix Ginseng, Radix Ophiopogonis; Fructus Schisandrae Chinensis is formed; Have supplementing QI and nourishing YIN, multiple arteries and veins takes off admittedly, clinically is usually used in treating that acute myocardial infarction, cardiogenic shock, toxic shock, hemorrhagic shock and coronary heart disease, endocrine disturbance etc. are sick belongs to the deficiency of both QI and YIN persons.
Existing qi-tonifying and pulse-restoring preparation extraction process is following:
Chinese patent CN200310116732.9 discloses a kind of method that injection is given birth to the arteries and veins lyophilized injectable powder for preparing; Earlier to Fructus Schisandrae Chinensis with 60-80% alcohol reflux 2-3 time, each 60-120min reclaims that ethanol to thing is pure distinguishes the flavor of; Extractum under agitation adds ethanol; Make the alcohol amount of containing reach 60-80%, filter, concentrating under reduced pressure gets extracting solution A; Fructus Schisandrae Chinensis medicinal residues and Radix Ginseng behind the reuse ethanol extraction add hydration and fry in shallow oil Radix Ophiopogonis, after the merging aqueous extract concentrates; Again through precipitate with ethanol, filter, reclaim behind the ethanol doubly measure deionized water dissolving with 10-15 after; Handle with macroporous adsorbent resin, be washed till sugar-free with deionized water earlier, be washed till no Saponin with ethanol again and end; Reclaim alcohol eluen, concentrate extracting solution B; With above-mentioned Radix Ginseng, the deposition adding distil water behind Radix Ophiopogonis and the Fructus Schisandrae Chinensis water extract-alcohol precipitation dissolves, and filters, and drying gets water and carries polysaccharide crude, with the dissolving of polysaccharide crude adding distil water, crosses hollow fiber column ultrafilter, gets extracting solution C.
Chinese patent CN200410057331.5 discloses a kind of method for preparing Shengmai injection, and Radix Ginseng is with ethanol extraction three times, merge extractive liquid,, concentrating under reduced pressure; Add water to medical material than 1: 1, separate and to remove upper strata oil, filter; Concentrate, vacuum drying gets Radix Ginseng extract; The decocte with water respectively of Radix Ophiopogonis and Fructus Schisandrae Chinensis, merge extractive liquid,, concentrating under reduced pressure adds ethanol, places, and filters; Filtrate decompression concentrates, and adds ethanol again, places, and filters, and regulates pH value, places; Filter, adjust pH again, reclaim under reduced pressure, vacuum drying, respectively Radix Ophiopogonis, Fructus Schisandrae Chinensis extrat.
Chinese patent CN200410021920.8 discloses a kind of method for preparing of qi-tonifying and pulse-restoring preparation, and Radix Ginseng Rubra is used ethanol extraction, reclaims ethanol, and aqueous precipitation filters, and concentrates, and vacuum drying, spray drying or lyophilization get Radix Ginseng Rubra extract; Radix Ophiopogonis, Fructus Schisandrae Chinensis are used water extraction, and after extracting solution concentrated, precipitate with ethanol reclaimed ethanol, and medicinal liquid is concentrated, and reuse vacuum drying, spray drying or freeze-drying are dry, get Radix Ophiopogonis, Fructus Schisandrae Chinensis extrat.
Chinese patent CN96117457.9 discloses a kind of SHENGMAI ZHUSHEYE and preparation method thereof, gets Radix Ginseng Rubra and mixes with Radix Ophiopogonis, uses alcohol dipping, filters, and alcohol reflux extracts; Fructus Schisandrae Chinensis with vapor distillation, decocting boil, alcohol deposition extracts.
Chinese patent CN 200310121126.6 discloses a kind of injection and has given birth to arteries and veins and preparation method thereof, and the source method is oozed in the medicinal component employing of Radix Ginseng Rubra, alcohol reflux boils the extraction that combines with decocting; The medicinal component of Radix Ophiopogonis adopts alcohol reflux to boil the extraction that combines with decocting; The medicinal component of Fructus Schisandrae Chinensis adopts water distillation, alcohol reflux to boil the extraction that combines with decocting; The extraction process flow process is following: (1) Radix Ginseng Rubra adopts ethanol to ooze the source earlier and extracts, and gets Radix Ginseng Rubra extracts active ingredients liquid a; To ooze the Radix Ginseng Rubra reuse alcohol reflux that extracted in the source subsequently, get Radix Ginseng Rubra extracts active ingredients liquid b; The Radix Ginseng Rubra of again alcohol reflux being crossed is used water boiling and extraction, filters, and filtrating concentrates the back precipitate with ethanol, gets supernatant then and filters; Filtrating merges with above-mentioned a, b, and cold preservation is got supernatant and filtered; Filtrating concentrates the back water precipitating, gets supernatant then and filters, and promptly gets the medicinal component extracting solution of Radix Ginseng Rubra C; (2) adopt alcohol reflux Radix Ophiopogonis earlier, cold preservation is got supernatant and is filtered, and filtrating concentrates the back water precipitating, gets supernatant again and filters, and promptly gets extracts active ingredients liquid d Radix Ophiopogonis; Use water boiling and extraction the Radix Ophiopogonis that again alcohol reflux is crossed, filter, filtrating concentrates the back precipitate with ethanol; Get supernatant then and filter, reclaim ethanol and be concentrated into thick paste shape, water precipitating; Getting supernatant then filters; Promptly get medicinal component extracting solution e Radix Ophiopogonis: extracts active ingredients liquid d and e merge with Radix Ophiopogonis, filter, and promptly get medicinal component extracting solution f Radix Ophiopogonis; (3) the Fructus Schisandrae Chinensis distillate adopts Fructus Schisandrae Chinensis is used steam distillation, collects distillate and promptly gets Fructus Schisandrae Chinensis distillate g: the Fructus Schisandrae Chinensis behind the collection distillate adopts alcohol reflux, cold preservation earlier; Get supernatant and filter, filter and concentrate the back water precipitating, get supernatant and filter; Promptly get Fructus Schisandrae Chinensis extracts active ingredients liquid h: the Fructus Schisandrae Chinensis of again alcohol reflux being crossed is used water boiling and extraction, filters, and filtrating concentrates back secondary precipitate with ethanol; Get supernatant then and filter, reclaim ethanol and be concentrated into thick paste shape, water precipitating; Go supernatant to filter then, promptly get the medicinal component extracting solution of Fructus Schisandrae Chinensis i; Fructus Schisandrae Chinensis extracts active ingredients liquid g, h and i are merged, filter, promptly get the medicinal composition extracting solution of Fructus Schisandrae Chinensis j.
Chinese patent CN99114091.5 discloses medicine of a kind of cardiovascular diseases of treatment and preparation method thereof, Radix Ginseng, Radix Ophiopogonis, Fructus Schisandrae Chinensis is added hydration fry in shallow oil; Aqueous extract or eluent are through ethanol or acetone precipitation; Macroporous resin treatment; Ion exchange resin treatment; Separate the effective ingredient that obtains extract.
Chinese patent CN 200510088087.3 discloses a kind of Activating Pulse Injecting Preparation And Preparation Method, and the method for preparing of Radix Ginseng Rubra extract adopts conventional ethanol refluxing process to extract, and the method for preparing of Fructus Schisandrae Chinensis extrat is, gets the water extraction 3 times that schisandra chinensis medicinal material adds 6 times of amounts; Each 40min filters, and merging filtrate is concentrated into relative density 1.10～1.15; Add 95% ethanol and carry out ethanol precipitation twice, make for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter; Merging filtrate, decompression recycling ethanol to relative density 1.10～1.15 adds water and stirs, and leaves standstill; Filter, filtrating adding w/v is 0.4% active carbon, boils; Keep little 30nin that boils, cold slightly filtration, this technology is not extracted the volatile oil composition in the schisandra chinensis medicinal material; The method for preparing of Radix Ophiopogonis extract is: get medical material Radix Ophiopogonis, filter behind the decocting in water precipitate with ethanol, add water behind the filtrate recycling ethanol and stir, leave standstill; Filter, the A that must filtrate is dissolved in water the deposition of precipitate with ethanol, leaves standstill; Filter, get liquor B, will filtrate A and B merge; Add charcoal treatment, filter, this technology has been extracted the polysaccharide composition in medical material Radix Ophiopogonis.
Chinese patent CN2005100081662.7 discloses a kind of Activating Pulse Injecting Preparation And Preparation Method, with Radix Ginseng Rubra medical material section, measures 60%～90% alcohol reflux 1～5 time, each 0.5～3 hour with 5～10 times; Merge backflow, filter, decompression filtrate recycling ethanol to relative density is 1.10～1.15, and the gained thick paste adds water; Stir, 2～SOC left standstill 6～20 hours, filtered; Filtrating is used charcoal treatment, filters, and the A that must filtrate supplies dosing to use; Get each medical material of wheat, add 5～10 and borrow the water extraction 1～4 time of amount, each 20～90min filters, and it is 1.05～1.10 that filtrating is concentrated into relative density; Add 95% ethanol and carry out ethanol precipitation twice, make for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter, decompression filtrate recycling ethanol to relative density is 1.10～1.15; Add water, stir, put 6～20 hours, filter, get liquor B 1; The deposition of ethanol precipitation twice is merged, add the water for injection dissolving, left standstill 6～20 hours, filter, get liquor B 2; Liquor B 1 is merged with B2, and adding w/v is the charcoal treatment of 0.1-3%, filters, and gets liquor B and supplies dosing to use; Schisandra chinensis medicinal material adds the water extraction 1～4 time of 4～8 times of amounts, and each 20～90min filters, merging filtrate, and being concentrated into relative density is 1.10～1.15; The gained thick paste adds 95% ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, makes for the second time to contain the alcohol amount and reach 85%, filters merging filtrate; Decompression recycling ethanol to relative density is 1.10～1.15, adds water, stirs, and leaves standstill; Filter, filtrating filters with after the charcoal treatment, gets liquor C and supplies dosing to use; To filtrate A, B, C mixing are processed different preparations according to conventional method.
Complex manufacturing in the prior art, cost is high, and the problem that extraction ratio is low never is overcome.
The present invention has overcome the shortcoming of existing extraction process through research for a long time, and it is high to have invented a kind of effective component extraction rate, good stability, and dosage is few, instant effect, cost is low, is easy to the long-time method for distilling of preserving.
Summary of the invention:
The present invention provides a kind of new Yiqi and vein recovery extract and preparation method thereof.
Yiqi and vein recovery is by Radix Ginseng 1-50 part, Radix Ophiopogonis 3-150 part, the Chinese medicine preparation that Fructus Schisandrae Chinensis 1.5-75 part is prepared from according to certain extraction process, and the Yiqi and vein recovery prescription is preferably: 1 part of Radix Ginseng, 3 parts of Radix Ophiopogonis, 1.5 parts of Fructus Schisandrae Chinensis, said part is weight portion.
Wherein Radix Ginseng is Radix Ginseng Rubra or Radix Ginseng, preferred Radix Ginseng Rubra.Fructus Schisandrae Chinensis is Radix Schisandrae Bicoloris or Fructus Schisandrae Sphenantherae, preferred Radix Schisandrae Bicoloris.
Method for preparing of the present invention comprises together to be extracted two flavor Chinese medicines in 1 part of Radix Ginseng, 3 parts of Radix Ophiopogonis, 1.5 part of three flavor of the Fructus Schisandrae Chinensis Chinese medicine, and another Chinese medicine extracts separately.
Method for preparing of the present invention is with Radix Ginseng, and Fructus Schisandrae Chinensis closes to be carried, and singly carry Radix Ophiopogonis.
Method for preparing of the present invention, wherein said close to carry be meant that with Radix Ginseng, Fructus Schisandrae Chinensis mixes.With water extraction or use alcohol extraction.Said singly carrying is meant extracted separately with water extraction or with alcohol extraction Radix Ophiopogonis.
Wherein be meant to be soaked in water or water heating is boiled and carried or the water heating and refluxing extraction with water extraction,
Be meant that with alcohol extraction using the solvent that contains ethanol or other alcohols materials to use soaks, percolation, modes such as reflux are extracted, and wherein preferred alcohol is ethanol, and ethanol can be the ethanol of 30%-100% concentration.
Method for preparing of the present invention also comprises the further water of medicinal residues after extracting for the first time or for the second time or the step of alcohol extraction.
Method for preparing of the present invention also comprises the extraction product after extracting is separated the step with purification.Said separation and purification comprise deposition, filter extraction, washing, chromatography, ultrafilter membrane ultrafiltration, macroporous adsorbent resin method purification, methods such as recrystallization.
For this reason, method for preparing of the present invention preferably adopts following method
(1) Radix Ginseng, Fructus Schisandrae Chinensis close and carry, and extraction step is following:
Medical material adds water, transfers pH9～14, decoct three times, and each 30 minutes～1 hour, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing.
(2) singly carry Radix Ophiopogonis, extraction step is following:
The material of getting it filled is used the 60-95% ethanol percolation, and residue volatilizes ethanol, adds 4-8 times of water gaging reflux, extract, 1-3 time, each 30 minutes-2 hours; The water extract is concentrated into every milliliter and contains crude drug 0.2g, and it is 80% that adding ethanol alcohol is sink to concentration, leaves standstill, and filters; Deposition is used an amount of dehydrated alcohol, and acetone washs successively, 50 ℃ of vacuum dryings; Get crude polysaccharides Radix Ophiopogonis, the product that obtains is for singly carrying thing
More than close to carry thing and singly propose the thing merging and be extract of the present invention.
The most preferred method for preparing of the present invention in an embodiment.
The present invention also provides the pharmaceutical composition that is prepared into as active constituents of medicine with extract of the present invention, and pharmaceutical composition of the present invention comprises extract, and said composition can also add the medicine acceptable carrier as required.
Compositions of the present invention is the pharmaceutical dosage forms of UD, and said unit dosage form is meant the unit of preparation, as every of tablet, and capsular every capsules, every bottle of oral liquid, every bag of granule etc.
Compositions of the present invention active component wherein, its shared percentage by weight in preparation can be 0.1-99.9%, all the other are the medicine acceptable carrier.
Compositions of the present invention obtains through above-mentioned active component and medicine acceptable carrier are mixed with.
Compositions of the present invention; Its pharmaceutical dosage forms can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably injection, more preferably freeze-dried powder.
Compositions of the present invention, the preparation of its oral administration can contain excipient commonly used, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet in case of necessity.
The filler that is suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of appropriate drug comprises sodium lauryl sulphate.
Can fill through mixing, the method that tabletting etc. are commonly used prepares solid oral composition.Mix repeatedly active substance is distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid for example can be aqueous or oily suspensions, solution, Emulsion, syrup or elixir, perhaps can be a kind of available water before use or other suitable composite dry products of carrier.This liquid preparation can contain conventional additive; Such as suspending agent; For example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat; Emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if desired, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, can this chemical compound be suspended or dissolving.The preparation of solution is normally through being dissolved in active substance in a kind of carrier filter-sterilized before it is packed into a kind of suitable bottle or ampoule, sealing then.For example a kind of local anesthetic of adjuvant, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be after the bottle of packing into that this compositions is freezing, and under vacuum, water is removed.
Compositions of the present invention; When being prepared into medicament, optionally add suitable medicine acceptable carrier; Said medicine acceptable carrier is selected from: meglumine, mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDTA disodium, EDTA calcium sodium, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, soil temperature 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Compositions of the present invention is confirmed usage and dosage according to patient's situation in use, but obeys every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet.
Beneficial effect of the present invention:
Through method for distilling of the present invention, the extract that obtains meets the requirements through the resin inspection; Ginsenoside and schisandrin content are high in the extract; Impurity is few.
Get crude polysaccharides Radix Ophiopogonis, adopt phenol-concentrated sulphuric acid method to record Radix Ophiopogonis polysaccharide content 11.50%.
(polysaccharide determination adopts: the phenolsulfuric acid method, like the extraction and the assay kind subworld 2004 of seed polysaccharide Radix Ophiopogonis such as literary composition, 12:24-26 referring to Zhao Hongqiao Zhu Jiong ripple Dong; Deoxyschizandrin and schisandrin B content assaying method referring to: Wang Ru Zhang Yancheng builds the preferred south China national defence medical journal 2006,19 (6) of schisandra active component extraction processes such as peak: 4-6,47)
Solution stability testing:
With the extract of embodiment 1,2 or 3, add meglumine; 100 ℃ were heated 1 hour, and cold preservation is spent the night, and added mannitol, filtered standardize solution; Get solution 2ml, canned transparent peace bottle, sealing, the solution lucifuge is placed, at 1 day, 3 days, 10 days, 30 days range estimation clarity, the result sees the following form.
Table 1 solution stability testing
Method for distilling effective component extraction rate of the present invention is high, and cost is low, and is simple to operate, and finished product stability is good, and dosage is few, and curative effect is high, and instant effect is easy to long-time preservation.
Concrete true mode:
To combine embodiments of the invention further explain flesh and blood of the present invention below, this embodiment only is used to the present invention is described and the present invention is not limited.
Embodiment 1
(1) Radix Ginseng, Fructus Schisandrae Chinensis close and carry, and extraction step is following:
Medical material adds water, transfers pH9, decoct three times, and each 30 minutes, merge extractive liquid;
Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing.
(2) singly carry Radix Ophiopogonis, extraction step is following:
Get it filled material with 95% ethanol percolation defat, and residue volatilizes ethanol in the post, adds 8 times of water gaging hot refluxs and extracts each 30 minutes 2 times; The water extract is concentrated into every milliliter and contains crude drug 0.2g, and it is 80% that adding ethanol alcohol is sink to concentration, leaves standstill, and filters; Deposition is used an amount of dehydrated alcohol, and acetone washs successively, 50 ℃ of vacuum dryings; Get crude polysaccharides Radix Ophiopogonis, the product that obtains is for singly carrying thing
More than close to carry thing and singly propose the thing merging and be extract of the present invention.
Embodiment 2
(1) Radix Ginseng, Fructus Schisandrae Chinensis close and carry, and extraction step is following:
Medical material adds water, transfers pH14, decoct three times, and each 1 hour, merge extractive liquid;
Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing.
(2) singly carry Radix Ophiopogonis, extraction step is following:
Get it filled material with 95% ethanol percolation defat, and residue volatilizes ethanol in the post, adds 8 times of water gaging hot refluxs and extracts 2 times, each 30min; The water extract is concentrated into every milliliter and contains crude drug 0.2g, and it is 80% that adding ethanol alcohol is sink to concentration, leaves standstill, and filters; Deposition is used an amount of dehydrated alcohol, and acetone washs successively, 50 ℃ of vacuum dryings; Get crude polysaccharides Radix Ophiopogonis, the product that obtains is for singly carrying thing
More than close to carry thing and singly propose the thing merging and be extract of the present invention.
Embodiment 3
(1) Radix Ginseng, Fructus Schisandrae Chinensis close and carry, and extraction step is following:
Add 4 times of amounts of crude drug amount, 30% ethanol, transfer pH 9, reflux, extract,, each 1 hour, extract 1 time, the product that obtains is carried thing for closing.
(2) the Radix Ginseng list is carried, and extraction step is following:
Get the Radix Ophiopogonis medical material and use 90% ethanol percolation, remove alcohol behind the percolation, well-established law water is carried 3 times, and each 40 minutes, filtered while hot, filtrating adds 0.2% active carbon and stirs hold over night; The extracting solution of filtrating carbon removal is evaporated to 1/10 of original volume, adds 95% alcohol chromatography, makes to contain alcohol amount and reach 80%; Leave standstill and separate out deposition, alcohol is analysed thing and is drained, and uses dehydrated alcohol successively; Washing with acetone, 60 ℃ dry canescence Radix Ophiopogonis polysaccharide amorphous powder, for singly carrying thing.
More than close to carry thing and singly propose the thing merging and obtain extract.
Embodiment 4
The preparation of lyophilized formulations:
(1) with the extract of embodiment 1,2 or 3, adds meglumine; 100 ℃ were heated 1 hour, and cold preservation is spent the night, and added mannitol, filtered standardize solution;
(2) activated carbon decolorizing;
(3) moisturizing;
(4) aseptic filtration;
(5) fill;
(6) lyophilizing.
Embodiment 5
The preparation of other preparations:
With the extract of embodiment 1,2 or 3, add carrier, press the galenic pharmacy routine techniques, be prepared into tablet, capsule, granule, dosage forms such as oral liquid.
Claims (5)
1. Yiqi and vein recovery preparation method of extract, said Yiqi and vein recovery extract is by 1 part of Radix Ginseng, and 3 parts of Radix Ophiopogonis, Fructus Schisandrae Chinensis is processed for 1.5 parts; It is characterized in that said method for preparing comprises Radix Ginseng, Fructus Schisandrae Chinensis closes to be carried; Extraction step is following: get Radix Ginseng, Fructus Schisandrae Chinensis adds water, transfers pH to 9～14; Decoct three times, each 30 minutes～1 hour, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing; Singly carry Radix Ophiopogonis, and extraction step is following: get and use the 60-95% ethanol percolation Radix Ophiopogonis, residue volatilizes ethanol, adds 4-8 times of water gaging reflux, extract, 1-3 time; Each 30 minutes-2 hours, the water extract was concentrated into every milliliter and contains crude drug 0.2g, and adding ethanol to concentration is 80%; Leave standstill, filter, deposition is used dehydrated alcohol; Acetone washs successively, 50 ℃ of vacuum dryings, and the product that obtains is for singly carrying thing; More than close and carry thing and singly carry thing and merge into extract.
2. the described method for preparing of claim 1 is characterized in that, (one) Radix Ginseng, Fructus Schisandrae Chinensis close and carry, and extraction step is following: get Radix Ginseng, Fructus Schisandrae Chinensis adds water, transfers pH9, decoct three times, and each 30 minutes, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing; (2) singly carry Radix Ophiopogonis, extraction step is following: get Radix Ophiopogonis with 95% ethanol percolation defat, residue volatilizes ethanol in the post, adds 8 times of water gaging hot refluxs and extracts 2 times; Each 30min, the water extract is concentrated into every milliliter and contains crude drug 0.2g, and it is 80% that adding ethanol alcohol is sink to concentration, leaves standstill; Filter, deposition is used an amount of dehydrated alcohol, and acetone washs successively; 50 ℃ of vacuum dryings, the product that obtains is carried thing and is singly carried thing and merge into extract for singly carrying thing, more than closing.
3. the described method for preparing of claim 1 is characterized in that, Radix Ginseng, Fructus Schisandrae Chinensis close to be carried, and extraction step is following: get Radix Ginseng, Fructus Schisandrae Chinensis adds water, transfers pH14, decoct three times, and each 1 hour, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing; Singly carry Radix Ophiopogonis, and extraction step is following: get Radix Ophiopogonis with 95% ethanol percolation defat, residue volatilizes ethanol in the post, adds 8 times of water gaging hot refluxs and extracts 2 times; Each 30min, the water extract is concentrated into every milliliter and contains crude drug 0.2g, and it is 80% that adding ethanol alcohol is sink to concentration, leaves standstill; Filter, deposition is used an amount of dehydrated alcohol, and acetone washs successively, 50 ℃ of vacuum dryings; Radix Ophiopogonis crude polysaccharides, the product that obtains is carried thing and is singly carried thing and merge into extract for singly carrying thing, more than closing.
4. the described method for preparing of claim 1 is characterized in that, Radix Ginseng, Fructus Schisandrae Chinensis close to be carried, and extraction step is following: get Radix Ginseng, Fructus Schisandrae Chinensis adds water, transfers pH to 9～14, decoct three times, and each 30 minutes～1 hour, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing; Singly carry Radix Ophiopogonis, and extraction step is following: get and use 60% ethanol percolation Radix Ophiopogonis, residue volatilizes ethanol, adds 4 times of water gaging reflux, extract, 2 times; Each 30 minutes, the water extract was concentrated into every milliliter and contains crude drug 0.2g, and adding ethanol to concentration is 80%; Leave standstill, filter, deposition is used dehydrated alcohol; Acetone washs successively, 50 ℃ of vacuum dryings, and the product that obtains is for singly carrying thing; More than close and carry thing and singly carry thing and merge into extract.
5. the described method for preparing of claim 1 is characterized in that, Radix Ginseng, Fructus Schisandrae Chinensis close to be carried, and extraction step is following: get Radix Ginseng, Fructus Schisandrae Chinensis adds water, transfers pH to 9～14, decoct three times, and each 30 minutes～1 hour, merge extractive liquid; Successively with 75% ethanol and 85% ethanol cold preservation deposition, filtration, decompression recycling ethanol to relative density is 1.1～1.20, the product that obtains is carried thing for closing; Singly carry Radix Ophiopogonis, and extraction step is following: get and use 80% ethanol percolation Radix Ophiopogonis, residue volatilizes ethanol, adds 6 times of water gaging reflux, extract, 1 time; 2 hours, the water extract was concentrated into every milliliter and contains crude drug 0.2g, and adding ethanol to concentration is 80%; Leave standstill, filter, deposition is used dehydrated alcohol; Acetone washs successively, 50 ℃ of vacuum dryings, and the product that obtains is for singly carrying thing; More than close and carry thing and singly carry thing and merge into extract.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559564A (en) * | 2004-02-24 | 2005-01-05 | 贵州益佰制药股份有限公司 | Supplementing qi pulse restoring Chinese medicinal preparation and its preparation method |
| CN1626200A (en) * | 2004-08-05 | 2005-06-15 | 贵阳云岩西创药物科技开发有限公司 | Injection preparation for generating channels and vessels, and preparation method |
| CN1628793A (en) * | 2004-08-27 | 2005-06-22 | 张正生 | Injectio for activating pulse and its preparing method |
| CN1720978A (en) * | 2004-08-05 | 2006-01-18 | 贵阳云岩西创药物科技开发有限公司 | Pulse invigorating injection and method for preparing the same |
| CN1733209A (en) * | 2004-08-05 | 2006-02-15 | 贵阳云岩西创药物科技开发有限公司 | Injection for restoring pulse beat and process for preparing the same |
| CN1781537A (en) * | 2004-08-05 | 2006-06-07 | 贵阳云岩西创药物科技开发有限公司 | Shengmai injection and its preparing method |
| CN1814161A (en) * | 2005-02-05 | 2006-08-09 | 北京国药苑科技有限公司 | Qi-tonifying and pulse-retaliation tablet preparing method |
-
2008
- 2008-12-05 CN CN 200810153783 patent/CN101745002B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559564A (en) * | 2004-02-24 | 2005-01-05 | 贵州益佰制药股份有限公司 | Supplementing qi pulse restoring Chinese medicinal preparation and its preparation method |
| CN1626200A (en) * | 2004-08-05 | 2005-06-15 | 贵阳云岩西创药物科技开发有限公司 | Injection preparation for generating channels and vessels, and preparation method |
| CN1720978A (en) * | 2004-08-05 | 2006-01-18 | 贵阳云岩西创药物科技开发有限公司 | Pulse invigorating injection and method for preparing the same |
| CN1733209A (en) * | 2004-08-05 | 2006-02-15 | 贵阳云岩西创药物科技开发有限公司 | Injection for restoring pulse beat and process for preparing the same |
| CN1781537A (en) * | 2004-08-05 | 2006-06-07 | 贵阳云岩西创药物科技开发有限公司 | Shengmai injection and its preparing method |
| CN1628793A (en) * | 2004-08-27 | 2005-06-22 | 张正生 | Injectio for activating pulse and its preparing method |
| CN1814161A (en) * | 2005-02-05 | 2006-08-09 | 北京国药苑科技有限公司 | Qi-tonifying and pulse-retaliation tablet preparing method |
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国卫生部药典委员会编.生脉注射液.《卫生部颁药品标准（中药成方制剂第十五册）》.1998, * |
| 苏彦珍等.不同工艺路线制备生脉口服液的比较.《中国中药杂志》.1999,第24卷(第2期), * |
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