CN101744948A - Extract from fruit shell of camellia oleifera abel and preparation method and application thereof - Google Patents
Extract from fruit shell of camellia oleifera abel and preparation method and application thereof Download PDFInfo
- Publication number
- CN101744948A CN101744948A CN200810207353A CN200810207353A CN101744948A CN 101744948 A CN101744948 A CN 101744948A CN 200810207353 A CN200810207353 A CN 200810207353A CN 200810207353 A CN200810207353 A CN 200810207353A CN 101744948 A CN101744948 A CN 101744948A
- Authority
- CN
- China
- Prior art keywords
- extract
- camellia oleifera
- oleifera abel
- shell
- fruit shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an extract from the fruit shell of camellia oleifera abel and a preparation method and application thereof. Based on the total weight of the extract, the extract contains 5-70 w/weight percent of general flavone (in rutin); and camellia oleifera abel is the fruit shell of the woody plant camellia oleifera abel of the Camellia L category of the theaceae.
Description
Technical field
The present invention relates to the plant extract field.Relate more specifically to effective site of from the shell of oil tea fruit, extracting and its production and use.
Background technology
Oil tea (Camellia oleifera Abel) is Theaceae (Theaceae) Camellia (Camellia) xylophyta, is the distinctive oil plant seeds of China, mainly is distributed in the ground such as Jiangxi, Hunan, Zhejiang, Guangxi, Guangdong, Fujian, Guizhou of China.Oil tea can be divided into and spends oil tea, Flos Carthami oil tea and Hemerocallis citrina Baroni oil tea in vain by the color difference of flower, plant the widest in spending Oleum Camelliae in vain, wherein the widest with common oil tea distribution.Camellia oil contains abundant unsaturated fatty acid, and very high nutritive value is arranged, and is described as the olive oil in east.Present Chinese oil tea cultivated area increases just year by year, and the output of Semen Camelliae is also progressively increasing.
Shell of Camellia oleifera Abel claims tea bag again, is the shell of oil tea fruit, accounts for 2/3 of oil tea fruit gross weight, is the garbage that oil tea produces, and annual output has more than 170 ten thousand tons approximately.In the oil tea producing region, shell of Camellia oleifera Abel or act as a fuel or be dropped utilization rate extremely low [Chinese oil, 1996,21 (4): 39-42].The research of relevant shell of Camellia oleifera Abel has bibliographical information to utilize shell of Camellia oleifera Abel to produce potassium carbonate and potassium pyrophosphate, and its further investigation utilization is not appeared in the newspapers.As seen to the development and utilization of shell of Camellia oleifera Abel to revitalizing camellia oleiferaindustry, increase the added value of oil tea, realize the comprehensive utilization of oil tea by-product, have profound significance.
Fatty acid synthase (Fatty Acid Synthase, FAS), it is the key enzyme of the synthetic endogenous long-chain fatty acid of catalysis S-acetyl-coenzyme-A and malonyl coenzyme A, studies show that it is the potential target spot of treatment obesity and cancer, research about it more and more causes people's attention, and its inhibitor more becomes focus in the research because of its potential fat-reducing and anticancer function.Cerulenin (Cerulenin) is found the earliest FAS inhibitor, but its chemical constitution instability has certain toxicity, has seriously limited its range of application, the inhibition mechanism of Kuhajda etc. simulation cerulenin, synthetic and filtered out the comparatively stable FAS inhibitor C 75 of chemical constitution.Along with the favor of people to natural product, people more and more wish to seek efficient fatty acid synthase inhibitor from natural product.
This area presses for provides the active substance that more derives from natural plants, as efficient FAS inhibitor, especially comes from the active substance of the natural material that is abandoned in a large number at present.
Summary of the invention
The present invention aims to provide a kind of extract from fruit shell of camellia oleifera abel.
Second purpose of the present invention provides the preparation method of described extract from fruit shell of camellia oleifera abel.
The 3rd purpose of the present invention provides the purposes of described extract from fruit shell of camellia oleifera abel.
The 4th purpose of the present invention provides a kind of compositions that contains described extract from fruit shell of camellia oleifera abel.
In a first aspect of the present invention, a kind of extract from fruit shell of camellia oleifera abel is provided, in the extract total amount, contain 5-70w/w% total flavones (in rutin) in the described extract; Described shell of Camellia oleifera Abel is the shell of the fruit of Theaceae (Theaceae) Camellia (Camellia L) xylophyta oil tea.
In another preference, described extract from fruit shell of camellia oleifera abel has two ultraviolet absorption peaks at 285nm and 236nm place.
In another preference, described extract is the material that extracts through alcohol-aqueous solvent, and described alcohol is selected from methanol or ethanol; More preferably, described extract is to be the material that the alcohol-aqueous solvent of 0-100v/v% extracts through determining alcohol, and described alcohol is selected from methanol or ethanol.
In another preference, described extract from fruit shell of camellia oleifera abel obtains as follows:
(1) shell of Camellia oleifera Abel and alcohol-aqueous solvent is mixed, extract and obtain extract from fruit shell of camellia oleifera abel with being selected from one or more following modes; Lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction or microwave radiation extraction.
In another preference, described extracting condition is: extracting temperature is 20-100 ℃, extraction time 0.1-5 hour, and solid-liquid ratio W/V:1: 3-30; The condition of described microwave radiation extraction is: microwave irradiation power is 100-6000W, and radiated time is that (more preferably, 2min-1h), solid-liquid ratio was 1: 3-1: 30 (W/V) in 20 seconds-5 hours; The condition of described ultrasonic extraction is: solid-liquid ratio 1: 3-1: 30 (W/V), ultrasonic power 50-5000W, ultrasound wave 12-180 action time minute.
In another preference, after step (1), also comprise the steps:
(2) separation and purification; Described separation and purification is selected from column chromatography or membrance separation.
In a second aspect of the present invention, a kind of preparation method of aforesaid extract from fruit shell of camellia oleifera abel is provided, described method comprises step:
(1) shell of Camellia oleifera Abel and alcohol-aqueous solvent is mixed, extract and obtain extract from fruit shell of camellia oleifera abel with being selected from one or more following modes; Lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction or microwave radiation extraction.
In another preference, the concentration of alcohol is 0-100v/v% in described alcohol-aqueous solvent; Described extracting condition is: extracting temperature is 20-100 ℃, extraction time 0.1-5 hour, and solid-liquid ratio W/V:1: 3-30; The condition of described microwave radiation extraction is: microwave irradiation power is 100-6000W, and radiated time is 20 seconds-5 hours (more preferably, being 2min-1h), and solid-liquid ratio is 1: 3-1: 30 (W/V); The condition of described ultrasonic extraction is: solid-liquid ratio 1: 3-1: 30 (W/V), ultrasonic power 50-5000W, ultrasound wave 12-180 action time minute.
In another preference, after step (1), also comprise the steps:
(2) separation and purification; Described separation and purification is selected from column chromatography or membrance separation.
In another preference, the condition of described membrance separation is: adopt the rolling ultrafiltration membrane system, operating pressure is 0.4-1.2Mpa, operative temperature 20-60 ℃.
In another preference, described column chromatography filler is selected from polyamide, silica gel, macroporous resin, cellulose, agarose gel or polydextran gel.
In a third aspect of the present invention, provide a kind of aforesaid extract from fruit shell of camellia oleifera abel to suppress fatty acid synthase in preparation, reduce the application in fat or the antioxidative compositions.
In another preference, that described extract from fruit shell of camellia oleifera abel also has is antibiotic, antiinflammatory, radioprotective, desensitization, allaying tiredness, alleviation stress, raise immunity, protection cardiovascular and cerebrovascular vessel, antitumor prophylaxis of cancer, whiten and/or promote the function of natural on-off cycles of hair growth.
In another preference, described compositions is functional food ingredient, dietary supplement, natural drug raw material or cosmetics functional component.
In a fourth aspect of the present invention, a kind of compositions is provided, in composition total weight, it contains percentage by weight is the aforesaid extract from fruit shell of camellia oleifera abel of 1-99% and acceptable carrier pharmaceutically or on the bromatology.
In another preference, described compositions is beverage, drinks, capsule, soft capsule, powder, tablet, granule, oral liquid, spray and cream, Emulsion, water preparation and mastic.
In another preference, described compositions is capsule, powder, tablet, granule, oral liquid, spray, cream, Emulsion, water preparation or extractum.
In another preference, described compositions is functional food ingredient, dietary supplement, natural drug raw material or cosmetics functional component.
In view of the above, the invention provides the active substance that derives from natural plants, especially come from the active substance of the natural material (as shell of Camellia oleifera Abel) that is abandoned in a large number at present.
Description of drawings
Fig. 1 is the uv-spectrogram of the extract from fruit shell of camellia oleifera abel XI that obtained among the embodiment 7, scans in the wave-length coverage of 200-400nm, and ultraviolet spectrogram shows at 285nm, 236nm place two tangible ultraviolet absorption peaks are arranged.
Fig. 2 be among the embodiment 7 the liquid phase collection of illustrative plates of a lot of extract from fruit shell of camellia oleifera abel; Wherein
A is the liquid phase collection of illustrative plates of shell of Camellia oleifera Abel 50% ethanol extraction IV; B is the liquid phase collection of illustrative plates of extract from fruit shell of camellia oleifera abel XIII.
Fig. 3 extract from fruit shell of camellia oleifera abel liquid phase collection of illustrative plates characteristic material UV scanning collection of illustrative plates; Wherein
A is that appearance time is the characteristic material of 10.9min in the shell of Camellia oleifera Abel liquid phase collection of illustrative plates; B is that appearance time is the characteristic material of 11.9min in the shell of Camellia oleifera Abel liquid phase collection of illustrative plates
Fig. 4 has shown the irreversible inhibitory action of extract from fruit shell of camellia oleifera abel to fatty acid synthase
The specific embodiment
The inventor passes through the further investigation to the garbage-shell of Camellia oleifera Abel of oil tea production, find that water solublity effective site wherein has good effect of weight reducing and antioxidation, flavone in this effective site is important active component, this effective site scans demonstration in the wave-length coverage of 200-400nm, there are two tangible ultraviolet absorption peaks at 285nm, 236nm place.Finished the present invention on this basis.
Extract from fruit shell of camellia oleifera abel
Extract from fruit shell of camellia oleifera abel provided by the invention is the shell from the fruit of Theaceae (Theaceae) Camellia (Camellia L) xylophyta oil tea---the mixture that obtains the shell of Camellia oleifera Abel.Can be used for oil tea of the present invention comprises: spend oil tea, Flos Carthami oil tea and Hemerocallis citrina Baroni oil tea in vain, more preferably for spending oil tea in vain, as common oil tea (Camellia oleifera Abel), Youxian County oil tea (Camellia yuhsienensis Hu), lobule oil tea (Camellia miocar pa Hu).
Extract from fruit shell of camellia oleifera abel provided by the invention is the water solublity effective site of shell of Camellia oleifera Abel, wherein contains polyphenol, flavone; Is 1-30w/w% with the shell of Camellia oleifera Abel butt in respect of imitating position content, and flavone is counted 5-70w/w% at the content of effective site with rutin.
Extract from fruit shell of camellia oleifera abel provided by the invention is to be the material that the alcohol-aqueous solvent of 0-100v/v% extracts through determining alcohol, and described alcohol is selected from methanol or ethanol.Described extracting mode is selected from lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction and/or microwave radiation extraction.
Extract from fruit shell of camellia oleifera abel provided by the invention has two ultraviolet absorption peaks at 285nm and 236nm place.When adopting the high-performance liquid chromatogram determination extract from fruit shell of camellia oleifera abel provided by the invention of following condition, retention time was respectively 4.2 minutes, and 7.8 minutes, 10.9 minutes, the area summation at 11.9 minutes peak was the 40-80% of all peak area sums:
Immobile phase: reversed phase chromatographic column (preferred C18);
Mobile phase: the mixed solution of acetonitrile and 0.5-2v/v% aqueous acetic acid;
Flow velocity: 1mL/min.
Preferably, described high-performance liquid chromatogram determination is a gradient elution: the volume ratio of acetonitrile and 0.5-2v/v% aqueous acetic acid changed to 1: 2 from 1: 9 in 0-60 branch clock time.
There is no particular limitation for the form of extract from fruit shell of camellia oleifera abel provided by the invention, for example can be Powdered, paste or liquid (comprising pasty state).
The preparation method of extract from fruit shell of camellia oleifera abel
Extract from fruit shell of camellia oleifera abel provided by the invention can prepare by following method:
(1) shell of Camellia oleifera Abel and alcohol-aqueous solvent is mixed, extract and obtain extract from fruit shell of camellia oleifera abel with being selected from one or more following modes; Lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction or microwave radiation extraction.
The shell of Camellia oleifera Abel raw material that the present invention adopts can carry out pretreatment, also can be without pretreatment.Preferred raw material is a 5-50 purpose fragment, and water content is below 10w/w%.
Extract from fruit shell of camellia oleifera abel provided by the invention is to extract by alcohol-aqueous solvent to obtain.Described alcohol-water solution is that percent by volume is the alcoholic solution of 0-100%, can use this area alcohols commonly used, as ethanol and methanol, preferred alcohol.Described extracting method is hot reflux extraction, countercurrent extraction, microwave-assisted extraction, ultrasound wave assisted extraction, or its combination.
In one embodiment of the invention, extracting condition is: extracting temperature is 20-100 ℃, extraction time 0.1-5 hour, and solid-liquid ratio W/V:1: 3-30.
In of the present invention-individual embodiment, the technological parameter that microwave-assisted extracts is: microwave irradiation power 100-6000W, radiated time are 20 seconds-5 hours, and solid-liquid ratio is 1: 3-30 (W/V); Preferably, microwave irradiation power is 200-4000W (more preferably being 500-4000W), and radiated time is 20 seconds-1 hour (more preferably being 20 seconds-15 minutes), and solid-liquid ratio is 1: 5-20 (W/V).
In one embodiment of the invention, ultrasound wave assisted extraction solid-liquid ratio 1: 3-30 (W/V), ultrasonic power 50-5000W, ultrasound wave 12-180 action time minute; Preferably, ultrasound wave assisted extraction solid-liquid ratio 1: 5-30 (W/V) or 1: 3-1: 20 (W/V), ultrasonic power 200-2000W, ultrasound wave 18-60 action time minute or 20-120 minute.
In one embodiment of the invention, hot reflux assisted extraction solid-liquid ratio 1: 3-30 (W/V), temperature 60-100 ℃, extraction time 0.3-5 hour; More preferably, hot reflux assisted extraction solid-liquid ratio 1: 3-30 (W/V), 80-100 ℃.
In another preference, after step (1), can also be aided with other high efficiency separation means and be further purified.Can use the method for this area routine to carry out separation and purification further, wherein preferred film is separated or column chromatography.
In one embodiment of the invention, the condition of described membrance separation is: adopt the rolling ultrafiltration membrane system, operating pressure is 0.3-2Mpa (preferably being 0.4-1.2Mpa), operative temperature 10-70 ℃ (preferably being 20-50 ℃).
In one embodiment of the invention, described column chromatography filler is selected from polyamide, silica gel or macroporous resin.
The purposes of extract from fruit shell of camellia oleifera abel
Extract from fruit shell of camellia oleifera abel provided by the invention has the fatty acid synthase of inhibition, reduces fat and antioxidative effect.
Extract from fruit shell of camellia oleifera abel provided by the invention effectively free radical resisting, antioxidation, antibiotic, antiinflammatory, radioprotective, desensitization, allaying tiredness, alleviation stress, raise immunity, adjusting lipid metabolism, fat-reducing, protection cardiovascular and cerebrovascular vessel, antitumor prophylaxis of cancer, whiten, promote natural on-off cycles of hair growth.Therefore, the present invention also provides the described extract from fruit shell of camellia oleifera abel that applies effective dose by the experimenter to needs to prevent, improve or treat the method for described disease.
When using, used extract from fruit shell of camellia oleifera abel effective dose can change with the order of severity of pattern of using and disease to be treated.Concrete condition decides according to experimenter's individual instances, perhaps skilled one be or scope that the nutritionist can judge in.
As used herein, term " effective dose " is meant and can produces function or amount active and that can be accepted by people or/or animal to people and/or animal.
The compositions that contains extract from fruit shell of camellia oleifera abel
The invention provides a kind of compositions, in described composition total weight, it contains percentage by weight is the extract from fruit shell of camellia oleifera abel provided by the invention of 1-99% and acceptable carrier pharmaceutically or on the bromatology.
In the present invention, various compositionss can be by method well known in the art preparation, can be with extract from fruit shell of camellia oleifera abel and acceptable carrier mixed preparing pharmaceutically or on the bromatology.The composition of acceptable carrier is applicable to people and/or animal and does not have excessive bad side reaction (as toxicity, stimulation and allergy) pharmaceutically or on the bromatology, and the material of rational benefit/risk ratio is promptly arranged.Described have pharmaceutically or can also comprise nutrition enhancer such as natural extract, vitamin and trace element such as Radix Glycyrrhizae extract and dietary fiber, dextrin on the bromatology in the acceptable carrier.
" pharmaceutically acceptable carrier " refers to be used for the treatment of the carrier of agent administration, comprises various excipient and diluent.This term refers to some medicament carriers like this: they itself are not necessary active component, and do not have undue toxicity after using.Suitable carriers is well known to those of ordinary skill in the art, can contain liquid, as water, saline, glycerol and ethanol.In addition, also may there be complementary material in these carriers, as filler, disintegrating agent, lubricant, fluidizer, effervescent, wetting agent or emulsifying agent, correctives, pH buffer substance etc.
In another optimal way of the present invention, acceptable carrier or excipient can contain on the described bromatology: filler, disintegrating agent, lubricant, fluidizer, effervescent, correctives, clad material, meals goods or slow releasing agent.
Dosage form for compositions of the present invention has no particular limits, and can be any dosage form that is applicable to that suckling is taken; Preferably, described dosage form can be selected from capsule, soft capsule, powder, tablet, granule, oral liquid, spray, cream, Emulsion, water preparation or mastic etc.
Compositions of the present invention comprises pharmaceutical composition, food compositions, Halth-care composition, food ingredient compositions, dietary supplement composition, natural drug feedstock composition or cosmetics functional component compositions; Also can be health beverage, drinks etc.As long as they contain or are made up of tea Pu extract basically.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can combination in any.All features that this case description is disclosed can with any composition forms and usefulness, each feature that is disclosed in the description can anyly provide the alternative characteristics of identical, impartial or similar purpose to replace.Therefore removing has special instruction, and the feature that is disclosed only is the general example of equalization or similar features.
Major advantage of the present invention is:
1, sets up effective extraction process, can determine the standardization of extract from fruit shell of camellia oleifera abel and related preparations;
2, can guarantee to reach three effective, safe, stable basic demands of medicine;
3, make full use of the shell of Camellia oleifera Abel that is dropped in the past, reduced the wasting of resources, helped environmental conservation;
4, the availability of Flos Camelliae Japonicae resource is provided, has improved economic benefit, helped increasing income of peasant.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.Unless otherwise indicated, otherwise all percentage ratio and umber by weight.
Unless otherwise defined, the same meaning that employed all specialties and scientific words and one skilled in the art are familiar with in the literary composition.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The usefulness that preferable implementation method described in the literary composition and material only present a demonstration.
Aluminum nitrate-sodium nitrite colorimetry
The drafting of standard curve: accurately take by weighing with the phosphorus pentoxide be the desiccant drying under reduced pressure (60 ℃, 0.09Mpa), use 60% dissolve with ethanol to the rutin standard substance of constant weight, be settled to 250mL, shake up, standard application liquid, place in the low temperature camera bellows, in time detect.Draw standard application liquid 1.0mL respectively, 2.0mL, 3.0mL, 4.0mL, 5.0mL, 6.0mL add to 10mL with 60% ethanol respectively in the 25mL volumetric flask, add 5%NaNO
2Solution 1.0mL shakes up, and places 6min, adds 10%Al (NO again
3)
3Solution 1.0mL adds 4%NaOH solution 10mL behind the 6min, mixing, and the ethanol of reuse 30% is settled to scale, shakes up, and measures absorbance A at wavelength 510nm place behind the placement 15min, gets the regression equation between rutin content y (mg/mL) and absorbance A.
The mensuration of flavones content in the sample: get liquid 1mL to be measured in the 25mL volumetric flask, the same standard substance of assay method are measured its absorbance A at the 510nm place, by the regression equation calculation flavones content.
Extract flavone content (%)=(flavone compound quality/extract quality) * 100%
Forint phenol colorimetry
The making of standard curve: accurately take by weighing gallic acid standard substance 25mg, with water dissolution and be settled to 250mL, the reference substance standard solution of 0.1mg/mL.Accurate control sample solution 0.2,0.4,0.6,0.8, the 1.0mL of drawing is in the 10mL volumetric flask, add 1mL forint phenol developer again, add 2mL mass fraction 15% sodium carbonate liquor after shaking up again, be settled to 10mL, measure A760, the drawing standard curve behind the reaction 2h under the room temperature.
The mensuration of polyphenol content in the sample: pipette extracting solution 0.5mL to be determined in the 25mL volumetric flask, pipette extracting solution 0.5mL after the dilution then in the 10mL volumetric flask, add 1mL forint phenol developer and 2mL mass fraction 15% sodium carbonate liquor and standardize solution successively, measure light absorption value by above-mentioned standard curve determination method, and calculate the gallic acid equivalant of total phenols according to standard curve.
The content of polyphenol (%) in the extract=(polyphenol content in the crude extract/crude extract quality) * 100%
High-performance liquid chromatogram determination
Waters 2695 high performance liquid chromatogram separative elements, Water s 2996 diode array detector; Chromatographic column: Luna C18 (200 * 4.6mm 100A, 5 μ); Mobile phase: acetonitrile/1% acetic acid; Flow velocity: 1mL/min; Sample size: 20 μ L; Gradient is as follows:
| Time (min) | ??0 | ??5 | ??15 | ??20 |
| 1% acetic acid (%) | ??90 | ??20 | ??30 | ??10 |
| Acetonitrile (%) | ??10 | ??80 | ??70 | ??90 |
The fatty acid synthase activity determination method
Full response is measured: surveying live body is 100mmol/L KH
2PO
4-K
2HPO
4Buffer, pH 7; Contain 1mmoL/L EDTA; 3 μ mol/L AcCoA; 10 μ mol/L MalCoA; 35 μ mol/L NADPH, 37 ℃ of constant temperature add 10 μ g FAS and start reaction, analyse general TU-1810 ultraviolet-visible spectrophotometer continuous monitoring 340nm light absorption variation with Beijing is general.The reaction cumulative volume is 2ml.Initial 2 minutes absorption values be changed to the initial velocity that straight line is represented this reaction.
The mensuration that fast combination (reversible) suppresses: tested extract sample is added to be surveyed in the live system, adds FAS again and starts reaction, and the activity of mensuration enzyme is Ai, and replacing the survey slip-knot of extract sample with extractant really is Ao.Ai/Ao is a residual activity, and the low more inhibition ability of this value is strong more.But this inhibition is generally retroactive inhibition.
The mensuration of 503nhibiting concentration: the fast combination of measuring under different extract dosages FAS suppresses, and surveys dry weight (μ g/ml) mapping that the solution of living contains extract with residual activity to every milliliter.Increase and to estimate the inhibition concentration that activity inhibited one half on the curve that reduces and be 503nhibiting concentration (IC50) with suppressing dosage by residual activity.The more little inhibition ability of this parameter value is strong more.
The slow rate constant that suppresses in conjunction with (irreversible) is measured: inhibitor is added in the FAS solution, and 25 ℃ of placements behind the mixing are got mixed solution at different intervals and measured enzymatic activity At, the Ao in contrast that lives of the survey when only adding the extractant of same amount.At/Ao be this moment residual activity (Remaining Activity, R.A.).
Embodiment 1
The ultrasonic assisted extraction extract from fruit shell of camellia oleifera abel of water I
(1) gets shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add the water of 10 times of weight, 60 ℃ of ultrasonic extraction 20 minutes;
(2) under the condition with 4000 rev/mins of said extracted liquid centrifugal 10 minutes, get supernatant, standardize solution is in order to using;
(3) get extracting solution, after 60 ℃ of oven dry, calculating extract from fruit shell of camellia oleifera abel I yield is 11.9% (w/w).
General flavone content is 3.05 grams in the dried tea Pu of per 100 grams of employing aluminum nitrate-sodium nitrite colorimetric method for determining, and general flavone content is 25.6% (in rutin) among the extract from fruit shell of camellia oleifera abel I.
Adopting forint phenol colorimetric method for determining total phenols yield is 2.31%, and total phenol content is 19.4% (in gallic acid) among the extract from fruit shell of camellia oleifera abel I.
Embodiment 2
With the ultrasonic assisted extraction extract from fruit shell of camellia oleifera abel of 50% ethanol II
(1) gets shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add 50% alcoholic solution of 10 times of weight, 30 ℃ of ultrasonic extraction 30 minutes;
(2) under the condition with 4000 rev/mins of said extracted liquid centrifugal 10 minutes, get supernatant, concentrate, spray drying, extract from fruit shell of camellia oleifera abel II.
Flavones content is 28.5% (in rutin) in employing aluminum nitrate-sodium nitrite colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Total phenol content is 21.4% (in gallic acid) in the employing forint phenol colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Embodiment 3
40% ethanol microwave-assisted extracts shell of Camellia oleifera Abel extract II I
(1) get shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add 40% alcoholic solution of 10 times of weight, the 700W microwave-assisted extracted 2 minutes;
(2) with said extracted liquid vacuum decompression sucking filtration, get supernatant, get extract from fruit shell of camellia oleifera abel III.
Flavones content is 29.8% (in rutin) in employing aluminum nitrate-sodium nitrite colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Total phenol content is 22.0% (in gallic acid) in the employing forint phenol colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Embodiment 4
Extract shell of Camellia oleifera Abel extract I V with 50% alcohol heat reflux
(1) gets shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add 50% alcoholic solution of 10 times of weight, 100 ℃ of hot reflux assisted extraction 2 hours;
(2) with said extracted liquid vacuum decompression sucking filtration 10 minutes, get supernatant, concentrate, spray drying gets extract from fruit shell of camellia oleifera abel IV.
Flavones content is 29.6% (in rutin) in employing aluminum nitrate-sodium nitrite colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Total phenol content is 22.4% (in gallic acid) in the employing forint phenol colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Embodiment 5
Extract from fruit shell of camellia oleifera abel XVII with 100% methanol ultrasound wave assisted extraction
(1) gets shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add 100% methanol solution of 10 times of weight, 30 ℃ of ultrasound wave assisted extraction 30 minutes;
(2) with said extracted liquid vacuum decompression sucking filtration 10 minutes, get supernatant, concentrate, spray drying gets extract from fruit shell of camellia oleifera abel XVII.
Flavones content is 29.4% (in rutin) in employing aluminum nitrate-sodium nitrite colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Total phenol content is 23.1% (in gallic acid) in the employing forint phenol colorimetric method for determining extract from fruit shell of camellia oleifera abel.
Embodiment 6
The purified extract from fruit shell of camellia oleifera abel V of macroporous resin
(1) get shell of Camellia oleifera Abel 50% ethanol extraction IV, water-soluble;
(2) get the D101 macroporous resin (available from the good scientific instrument company limited of Hangzhou moral) of clean grade, the dress post;
(3) behind the last sample,, collect the back concentrate drying respectively and get extract from fruit shell of camellia oleifera abel V respectively with water, 20% ethanol, 40% ethanol, 80% ethanol, two column volumes of 100% ethanol elution.
Adopt the flavones content (table 1) of aluminum nitrate-each elution fraction of sodium nitrite colorimetric method for determining.Wherein the flavones content of 40% ethanol elution part is the highest, reaches 54.94%, and the flavones content of water elution part is minimum, is 1.74%.
The flavones content of each component of the refining back of table 1 D101
| Component | Flavones content (%) |
| D101 water elution part | ??1.73 |
| |
??34.32 |
| |
??54.94 |
| |
??27.81 |
| D101 100% ethanol elution part | ??10.17 |
Embodiment 7
Shell of Camellia oleifera Abel different concentration ethanol water solution extract is to the reversible inhibition of fatty acid synthase
(1) gets shell of Camellia oleifera Abel, pulverize the back and cross 50 mesh sieves, place container, add the aqueous solution of 10 times of weight, 30 ℃ of ultrasonic extraction 30 minutes;
(2) under the condition with 4000 rev/mins of said extracted liquid centrifugal 10 minutes, get supernatant, the vacuum concentration drying, extract from fruit shell of camellia oleifera abel VI.
Aqueous solution in the said method is replaced with Different concentrations of alcohol-aqueous solvent (as 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%) respectively, and using the same method prepares extract from fruit shell of camellia oleifera abel VII, VIII, IX, X, XI, XII, XIII, XIV, XV, XVI again.
Active S-acetyl-coenzyme-A, malonyl coenzyme A, the NADPH of adopting of fatty acid synthase is the standard measuring method for activity mensuration of substrate, calculates the 503nhibiting concentration of extract to this enzyme.The result is as shown in table 2:
The extract of table 2 different solvents is to the 503nhibiting concentration of fatty acid synthase
| Extract from fruit shell of camellia oleifera abel | The alcohol-water solvent | Fatty acid synthase IC 50(μg/mL) | Flavones content (%) |
| ??VI | Water | ??1.72 | ??23.4 |
| ?? |
10% ethanol | ??1.39 | ??25.5 |
| ?? |
20% ethanol | ??1.33 | ??26.0 |
| ?? |
30% ethanol | ??1.17 | ??27.1 |
| ??X | 40% ethanol | ??0.96 | ??27.9 |
| ?? |
50% ethanol | ??0.96 | ??28.7 |
| ?? |
60% ethanol | ??1.00 | ??31.5 |
| ?? |
70% ethanol | ??1.57 | ??32.2 |
| ?? |
80% ethanol | ??1.68 | ??24.2 |
| ?? |
90% ethanol | ??3.96 | ??19.6 |
| ?? |
100% ethanol | ??3.28 | ??19.7 |
The result shows that along with the increase of extracting concentration of alcohol in the solvent, extract reduces first the enhancing afterwards of the inhibitory action of fatty acid synthase, and the inhibitory action of 40-50% ethanol extraction is the strongest; Along with the increase of concentration of alcohol, the flavones content in the extract also presents first increasing afterwards to be reduced, and wherein the flavones content of 70% ethanol extraction is the highest.
Flavones content in the extract from fruit shell of camellia oleifera abel and its inhibitory action to fatty acid synthase present certain dependency, suppress the strongest active material and be mainly polarity than large part, therefore after concentration of alcohol is elevated to a certain degree, is unfavorable for the extraction of this part material and extract is weakened the inhibitory action of fatty acid synthase.
Embodiment 8
Extract from fruit shell of camellia oleifera abel is to the irreversible inhibitory action of fatty synthase
(1) gets extract from fruit shell of camellia oleifera abel VI, be mixed with certain density solution;
(2) with after the insulation of a certain amount of extract from fruit shell of camellia oleifera abel VI solution and enzyme, from heat insulation system, get the mixture of enzyme and extract in the different time, add the residual activity of substrate S-acetyl-coenzyme-A, malonyl coenzyme A, NADPH mensuration enzyme.Sample and enzyme liquid are incubated, and mix sample concentration in the heat insulation system and be 0.025mg/ml through after 135 minutes, and enzyme is lived only remaining less than 30%, sees Fig. 4.
The result shows that there is very strong irreversible inhibitory action in extract from fruit shell of camellia oleifera abel VI to fatty acid synthase.
Embodiment 9
Extract from fruit shell of camellia oleifera abel is to the fat-reducing and antihyperglycemic of mice
(1) gets extract from fruit shell of camellia oleifera abel VI, be made into certain density solution;
(2) the ICR mice feeds normal diet 7d under experimental situation, weigh, be divided into 5 groups at random: normal control group, model group, positive controls (orlistat 50mg/kg), high dose group (extract from fruit shell of camellia oleifera abel VI 300mg/kg), low dose group (extract from fruit shell of camellia oleifera abel VI 150mg/kg).The normal control treated animal is fed with normal diet to end from the experiment beginning, and all the other each treated animals are all fed nutrient fodder (yolk powder 10%+ Adeps Sus domestica 10%+ normal diet 8%).Normal control group, model group are irritated stomach and give the equivalent distilled water every days, and all the other each groups are irritated stomach by above-mentioned dosage and give each trier every day;
(3) write down body weight weekly 1 time; After 6 weeks, each organizes mice fasting 12h, weigh, and the eye socket blood sampling, separation of serum, cervical vertebra dislocation is immediately put to death, and gets liver (being stored in the liquid nitrogen container after weighing) fast, separates testis, kidney fat on every side, weighs.
Concrete result of the test is shown in table 3, table 4, table 5, table 6.
Table 3 mice body weight change table
| The normal control group | Model group | Positive controls | High dose group | Low dose group | |
| Weight increase rate (%) | ??56.29 | ??67.51 | ??54.53 | ??39.28 | ??52.71 |
Table 4 mice Blood Lipid table
| The normal control group | Model group | Positive controls | High dose group | Low dose group | |
| ??TC(mmol/L) | ??2.81±0.25 | ??4.75±0.73 | ??4.14±0.41 | ??4.37±0.70 | ??4.03±0.52 |
| ??TG(mmol/L) | ??1.13±0.33 | ??1.50±0.25 | ??1.00±0.19 | ??0.69±0.11 | ??0.93±0.18 |
Fat weight around table 5 mice kidney week and the testis
| The normal control group | Model group | Positive controls | High dose group | Low dose group | |
| Fatty weight in wet base (g) around the kidney | ??0.09±0.04 | ??0.32±0.12 | ??0.18±0.08 | ??0.14±0.05 | ??0.247±0.07 |
| Fatty weight in wet base (g) around the testis | ??0.48±0.16 | ??1.07±0.36 | ??0.63±0.19 | ??0.56±0.15 | ??0.850±0.39 |
Table 6 mouse blood leptin level
| The normal control group | Model group | Positive controls | High dose group | Low dose group | |
| Leptin (ng/mL) | ??0.135±0.020 | ??0.431±0.048 | ??0.314±0.019 | ??0.307±0.036 | ??0.183±0.037 |
The result shows that extract from fruit shell of camellia oleifera abel can significantly alleviate the mice body weight, make mouse testis, kidney around fat mass obviously reduce, have blood lipid regulation effect significantly simultaneously.
The antioxidation of extract from fruit shell of camellia oleifera abel
Removing free radical (ABTS to extract from fruit shell of camellia oleifera abel
+, DPPH) ability measures.
(1) to the ABTS measured by esr technique
The preparation of ABTS working solution: prepare 7mmol/L ABTS (available from U.S. Sigma company) storing solution and 140mmol/L potassium peroxydisulfate (K2S2O8) solution earlier, get then and get ABTS after 5mL 7mmol/L ABTS storing solution and 88 μ L 140mmol/L potassium persulfate solutions mix
+Storing solution is again with this storing solution lucifuge deposit 12-16h at ambient temperature, at last with the ABTS that generates
+Storing solution dilutes with dehydrated alcohol, and making its absorbance under 30 ℃, 734nm wavelength is 0.70 ± 0.02, promptly obtains ABTS
+The free radical working solution.
The making of Trolox standard curve: accurately take by weighing Trol ox standard substance (available from U.S. Si gma company) 10mg, be settled to 10ml with dissolve with methanol, the titer of drawing 0,60,120,180,240,300,420 μ L respectively is diluted to 1mL with methanol.The ABTS that in test tube, adds 3.0mL
+The free radical working solution adds the standard solution of 30 μ L variable concentrations respectively, mixes, and 30 ℃ leave standstill 6min, read light absorption value (Abs) under the 734nm wavelength, with the concentration of Trolox the clearance rate of free radical are done standard curve.
Extract is removed the mensuration of the ability of ABTS free radical: the ABTS that adds 3.0mL in test tube
+The free radical working solution adds the testing sample of the 1mg/mL of 30 μ L again, mixes, and 30 ℃ leave standstill 6min, read light absorption value (Abs) under the 734nm wavelength, and obtains the corresponding Trolox equivalent concentration of testing sample on the Trolox standard curve, is defined as the TEAC value.The big more antioxidant antioxidant activity that shows of TEAC value is strong more.
The results are shown in Table 7.
The anti-ABTS free radical ability of removing of table 7 tea Pu extract
| Sample | ??TEAC(mg/L) |
| Tea Pu water extract VI | ??582.8 |
| Shell of |
??783.8 |
| Shell of |
??716.8 |
| Vitamin C | ??2000.2 |
The result shows, extract from fruit shell of camellia oleifera abel has the ability of very strong removing ABTS free radical, and wherein, extract XI has the ability of stronger removing ABTS free radical than VI, XIII, the TEAC equivalent of the extract XI of 1mg/mL is 783.8mg/L, but a little less than the ability of more ascorbic removing ABTS free radical.
(2) to the DPPH measured by esr technique
Adopt the removing free radical ability of DPPH assay extract from fruit shell of camellia oleifera abel.
The principle of this analytical method is: the DPPH free radical be a kind of stable be the free radical at center with nitrogen, there is absorption maximum at the place at the 515nm wavelength, its methanol solution is purple, and its concentration and absorbance are linear.When add free radical scavenger in the DPPH methanol solution after, free radical scavenger can or take place with the DPPH combined with radical to substitute, and DPPH free radical quantity is reduced, until reaching stable.
Specific analytical method is as follows:
Get liquid 2mL to be measured and 2 * 10
-4Mol/L DPPH solution 2mL adds in the same tool plug test tube and shakes up, and the at room temperature airtight 30min that leaves standstill makes reference with reagent blank, measures absorbance down in the 517nm wavelength, calculates the clearance rate of every kind of testing sample to the DPPH free radical according to following formula:
Clearance rate (%)=[1-(A
Sample-A
Sampleblank)/A
Control] * 100%
Wherein: A
SampleFor adding DPPH solution absorbency behind the extracting solution;
A
Sample blankAbsorbance for extracting solution;
A
ControlDPPH solution absorbency when not adding extracting solution.
With testing sample concentration is abscissa, is that vertical coordinate is drawn the curve that testing sample is removed the DPPH free radical with the clearance rate value.According to the equation of linear regression of curve, calculate the DPPH free radical scavenging activity and be 50% o'clock testing sample concentration, be defined as 503nhibiting concentration (IC
50).According to IC
50Judge the ability of testing sample removing DPPH free radical, IC
50Its removing free radical ability of more little expression is strong more.
The results are shown in Table 8.
The anti-DPPH free radical ability of removing of table 8 tea Pu extract
The result shows that extract from fruit shell of camellia oleifera abel XIII is strong than the removing DPPH free radical ability of XVI, and this may be relevant with its flavones content.
Embodiment 11
Compositions embodiment
Extract from fruit shell of camellia oleifera abel is allocated in the sun-proof substrate of cosmetics, according to following prescription and method preparing cosmetics.
Table 9 sun care preparations list of ingredients
Preparation method: A liquid and B liquid are heated to 72-82 ℃ respectively, and continuous stirring is all dissolved until various compositions, while stirring A liquid is added B liquid, continues to stir to be cooled to room temperature (15-30 ℃) until formed Emulsion, obtains the sun-proof standard substance of 100g at last.
Embodiment 12
Contain the effect of the cosmetic composition anti-ultraviolet radiation of extract from fruit shell of camellia oleifera abel
The cosmetics that embodiment 11 is obtained pass through to measure its SPF (sun protection factor) (sun protect factor, SPF) performance of evaluation tea Pu extract anti-ultraviolet radiation.
Utilize the SPF (sun protection factor) of adhesive tape method working sample.
1. the 3M adhesive tape is cut into 1.1cm * 4.5cm size, sticks on the quartz colorimetric utensil transparent side surface.
2. energized, the preheating spectrophotometer, selected multi-wavelength is measured, and measurement parameter is set at T% and Abs at twice respectively and sets ultraviolet wavelength and be respectively 290nm-400nm, and the wavelength interval is 5nm.
The quartz colorimetric utensil that 3. will post adhesive tape places sample light path and reference light paths, adjusts instrument zero.
4. weighing 9.9mg testing sample is evenly coated in sample on the quartz colorimetric utensil 3M adhesive tape.
5. the sample cuvette for preparing is put in the drying baker with 37 ℃ dry 15 minutes.
6. the testing sample cuvette is put with the sample light path in, get another quartz cell that posts adhesive tape and place reference light paths, measure the UVB district respectively and set wavelength ultraviolet absorbance value and absorbance, get the arithmetic equal value of respectively measuring numerical value.
7. 5 parallel sample of sequentially determining, computation of mean values is in the arithmetic mean of calculating average and be the absorbance of this sample and absorbance.
8. utilize the spf value of following formula calculation sample.
Wherein: E (λ): north latitude 40 degree, sun drift angle 20 degree, noon in summer the sunlight different wave length radiant intensity.
I (λ): be the erythemal effect coefficient of different wavelengths of light.
T (λ): be the absorbance of different wave length sample.
The spf value of the blank group of result is 8.72 ± 2.19, and the spf value of sample sets is 17.13 ± 3.38 (SPF 〉=15 are for super sun-proof), so tea Pu water extract VI has stronger anti-ultraviolet radiation performance.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. an extract from fruit shell of camellia oleifera abel is characterized in that, in the extract total amount, contains 5-70w/w% total flavones (in rutin) in the described extract; Described shell of Camellia oleifera Abel is the shell of the fruit of Theaceae (Theaceae) Camellia (CamelliaL) xylophyta oil tea.
2. extract from fruit shell of camellia oleifera abel as claimed in claim 1 is characterized in that, at 285nm and 236nm place two ultraviolet absorption peaks is arranged.
3. extract from fruit shell of camellia oleifera abel as claimed in claim 1 is characterized in that, described extract is the material that extracts through alcohol-aqueous solvent, and described alcohol is selected from methanol or ethanol; Preferred described extract is to be the material that the alcohol-aqueous solvent of 0-100v/v% extracts through determining alcohol, and described alcohol is selected from methanol or ethanol.
4. extract from fruit shell of camellia oleifera abel as claimed in claim 1 is characterized in that, described extract obtains as follows:
(1) shell of Camellia oleifera Abel and alcohol-aqueous solvent is mixed, extract and obtain extract from fruit shell of camellia oleifera abel with being selected from one or more following modes; Lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction or microwave radiation extraction.
5. the preparation method as the arbitrary described extract from fruit shell of camellia oleifera abel of claim 1-4 is characterized in that, described method comprises step:
(1) shell of Camellia oleifera Abel and alcohol-aqueous solvent is mixed, extract and obtain extract from fruit shell of camellia oleifera abel with being selected from one or more following modes; Lixiviate, hot reflux extraction, countercurrent extraction, ultrasonic extraction or microwave radiation extraction.
6. preparation method as claimed in claim 5 is characterized in that, the concentration of alcohol is 0-100v/v% in described alcohol-aqueous solvent; Described extracting condition is: extracting temperature is 20-100 ℃, extraction time 0.1-5 hour, and solid-liquid ratio W/V:1: 3-30; The condition of described microwave radiation extraction is: microwave irradiation power is 100-6000W, and radiated time is 20 seconds-5 hours (preferred 2min-1h), and solid-liquid ratio is 1: 3-1: 30 (W/V); The condition of described ultrasonic extraction is: solid-liquid ratio 1: 3-1: 30 (W/V), ultrasonic power 50-5000W, ultrasound wave 12-180 action time minute.
7. preparation method as claimed in claim 5 is characterized in that, also comprises the steps: after step (1)
(2) separation and purification; Described separation and purification is selected from column chromatography or membrance separation.
8. one kind is suppressed fatty acid synthase in preparation, reduces the application in fat or the antioxidative compositions as the arbitrary described extract from fruit shell of camellia oleifera abel of claim 1-4.
9. a compositions is characterized in that, in composition total weight, it contain percentage by weight be 1-99% as arbitrary described extract from fruit shell of camellia oleifera abel of claim 1-4 and acceptable carrier pharmaceutically or on the bromatology.
10. compositions as claimed in claim 9 is characterized in that, described compositions is beverage, drinks, capsule, soft capsule, powder, tablet, granule, oral liquid, spray and cream, Emulsion, water preparation and mastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810207353 CN101744948B (en) | 2008-12-19 | 2008-12-19 | Extract from fruit shell of camellia oleifera abel and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810207353 CN101744948B (en) | 2008-12-19 | 2008-12-19 | Extract from fruit shell of camellia oleifera abel and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101744948A true CN101744948A (en) | 2010-06-23 |
| CN101744948B CN101744948B (en) | 2013-09-18 |
Family
ID=42472963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810207353 Expired - Fee Related CN101744948B (en) | 2008-12-19 | 2008-12-19 | Extract from fruit shell of camellia oleifera abel and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101744948B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999521A (en) * | 2010-12-13 | 2011-04-06 | 中南林业科技大学 | Oil tea peel extract containing oil tea peel polyphenol as well as preparation method and application of oil tea peel extract |
| CN102139019A (en) * | 2011-03-26 | 2011-08-03 | 浙江大学 | Application of camellia oleifera peel extract |
| CN102210786A (en) * | 2011-06-08 | 2011-10-12 | 中国林业科学研究院林产化学工业研究所 | Method for extracting natural antioxidant from shells of camellia oleifera |
| CN103005162A (en) * | 2012-12-28 | 2013-04-03 | 清远容大生物工程有限公司 | Method for extracting antivirus growth promoter of saccharicterpenin |
| CN103030710A (en) * | 2013-01-05 | 2013-04-10 | 广西大学 | Resource utilization method of camellia oleifera shells |
| CN103689740A (en) * | 2013-12-12 | 2014-04-02 | 华南农业大学 | Extractive of camellia oleifera flowers and application of extractive in preparation of health-care drink |
| CN106667833A (en) * | 2016-11-10 | 2017-05-17 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN107397838A (en) * | 2017-08-30 | 2017-11-28 | 广西那坡县翠株园林业科技有限公司 | A kind of camellia fruit pico-ampere god's perfume (or spice) and preparation method thereof |
| CN107400015A (en) * | 2017-08-30 | 2017-11-28 | 广西那坡县翠株园林业科技有限公司 | A kind of camellia pericarp biological organic fertilizer and preparation method thereof |
| CN107674752A (en) * | 2017-09-30 | 2018-02-09 | 陈芹芳 | Primary camellia oil and its preparation technology |
| CN107669817A (en) * | 2017-10-09 | 2018-02-09 | 赣南医学院 | A kind of preparation method, product and its application of the anti-enteritis micro-capsule of oil-tea camellia husks |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101045111A (en) * | 2007-04-27 | 2007-10-03 | 浙江大学 | Inhibitor of fatty-acid synthase its preparing method and application |
-
2008
- 2008-12-19 CN CN 200810207353 patent/CN101744948B/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999521A (en) * | 2010-12-13 | 2011-04-06 | 中南林业科技大学 | Oil tea peel extract containing oil tea peel polyphenol as well as preparation method and application of oil tea peel extract |
| CN102139019A (en) * | 2011-03-26 | 2011-08-03 | 浙江大学 | Application of camellia oleifera peel extract |
| CN102210786A (en) * | 2011-06-08 | 2011-10-12 | 中国林业科学研究院林产化学工业研究所 | Method for extracting natural antioxidant from shells of camellia oleifera |
| CN102210786B (en) * | 2011-06-08 | 2012-09-05 | 中国林业科学研究院林产化学工业研究所 | Method for extracting natural antioxidant from shells of camellia oleifera |
| CN103005162A (en) * | 2012-12-28 | 2013-04-03 | 清远容大生物工程有限公司 | Method for extracting antivirus growth promoter of saccharicterpenin |
| CN103030710B (en) * | 2013-01-05 | 2015-03-18 | 广西大学 | Resource utilization method of camellia oleifera shells |
| CN103030710A (en) * | 2013-01-05 | 2013-04-10 | 广西大学 | Resource utilization method of camellia oleifera shells |
| CN103689740A (en) * | 2013-12-12 | 2014-04-02 | 华南农业大学 | Extractive of camellia oleifera flowers and application of extractive in preparation of health-care drink |
| CN106667833A (en) * | 2016-11-10 | 2017-05-17 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN106667833B (en) * | 2016-11-10 | 2019-08-09 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN107397838A (en) * | 2017-08-30 | 2017-11-28 | 广西那坡县翠株园林业科技有限公司 | A kind of camellia fruit pico-ampere god's perfume (or spice) and preparation method thereof |
| CN107400015A (en) * | 2017-08-30 | 2017-11-28 | 广西那坡县翠株园林业科技有限公司 | A kind of camellia pericarp biological organic fertilizer and preparation method thereof |
| CN107674752A (en) * | 2017-09-30 | 2018-02-09 | 陈芹芳 | Primary camellia oil and its preparation technology |
| CN107669817A (en) * | 2017-10-09 | 2018-02-09 | 赣南医学院 | A kind of preparation method, product and its application of the anti-enteritis micro-capsule of oil-tea camellia husks |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101744948B (en) | 2013-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101744948B (en) | Extract from fruit shell of camellia oleifera abel and preparation method and application thereof | |
| Wang et al. | Efficient extraction of flavonoids from Flos Sophorae Immaturus by tailored and sustainable deep eutectic solvent as green extraction media | |
| Zhang et al. | Aqueous two-phase extraction and enrichment of two main flavonoids from pigeon pea roots and the antioxidant activity | |
| Wong et al. | A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay | |
| Shao et al. | Analysis of conditions for microwave-assisted extraction of total water-soluble flavonoids from Perilla Frutescen s leaves | |
| Yang et al. | Extraction of protocatechuic acid from Scutellaria barbata D. Don using supercritical carbon dioxide | |
| CN102590433B (en) | A kind of quality determining method of the smooth preparation of liver | |
| CN104957512B (en) | A kind of desizing high anti-oxidation red yeast rice oat and the preparation method and application thereof | |
| CN101560265A (en) | Method for preparing oil-tea camellia husk polysaccharide and purifying method | |
| Mustafa et al. | Polyphenols, saponins and phytosterols in lentils and their health benefits: an overview | |
| CN101190281A (en) | Prune tree extract and preparation method and application thereof | |
| Rubab et al. | Determination of the GC–MS analysis of seed oil and assessment of pharmacokinetics of leaf extract of Camellia sinensis L. | |
| CN103235082B (en) | Method for detecting Jingwu capsule | |
| Zhao et al. | Enhanced extraction of isoflavonoids from Radix Astragali by incubation pretreatment combined with negative pressure cavitation and its antioxidant activity | |
| Mijangos Ricárdez et al. | Fast Ultrasound‐assisted Extraction of Polar (phenols) and Nonpolar (lipids) Fractions in Heterotheca inuloides Cass. | |
| CN103655844B (en) | The preparation method of Changshan grapefruit peel pomace extract and the preparation containing this extract | |
| Zhang et al. | The content of astilbin and taxifolin in concentrated extracts of Rhizoma Smilacis Glabrae and turtle jelly vary significantly | |
| CN101560266B (en) | Oil-tea camellia husk polysaccharide and application thereof | |
| Sun et al. | Quantitative analysis and comparison of four major flavonol glycosides in the leaves of Toona sinensis (A. Juss.) roemer (chinese toon) from various origins by high-performance liquid chromatography-diode array detector and hierarchical clustering analysis | |
| CN101921494B (en) | Preparation method of red pigment from camellia japonica | |
| Wang et al. | Peanut by-products utilization technology | |
| CN104971090A (en) | Application of cyclocarya paliurus effective part in preparation of medicine used for treating non-alcoholic fatty liver disease | |
| Jampa et al. | Multiple bioactivities of Manihot esculenta leaves: UV filter, anti-oxidation, anti-melanogenesis, collagen synthesis enhancement, and anti-adipogenesis | |
| Feng et al. | Optimization of natural deep eutectic solvents extraction of flavonoids from Xanthoceras sorbifolia Bunge by response surface methodology | |
| Kim et al. | Anti-aging and anti-diabetes effects of Aconitum pesudo-laeve var. erectum extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130918 Termination date: 20191219 |