CN101732291A - Application of dimethyl sulfoxide in preparing anti-aging products - Google Patents
Application of dimethyl sulfoxide in preparing anti-aging products Download PDFInfo
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- CN101732291A CN101732291A CN200910244038A CN200910244038A CN101732291A CN 101732291 A CN101732291 A CN 101732291A CN 200910244038 A CN200910244038 A CN 200910244038A CN 200910244038 A CN200910244038 A CN 200910244038A CN 101732291 A CN101732291 A CN 101732291A
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- dimethyl sulfoxide
- nematicide
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Abstract
The invention discloses a new application of dimethyl sulfoxide which is applied to preparing anti-aging products and nematoid life prolonging agents. The invention utilizes the biology having the mode of nematoid, detects by using a whole animal experiment, observes the influence of the dimethyl sulfoxide on the life of nematoid and finds that 0.5 to 2 percent of dimethyl sulfoxide can obviously prolong the average life and the maximum life of the nematoid.
Description
Technical field
The present invention relates to the new purposes of dimethyl sulfoxide slow down aging, particularly its application in the preparation anti-aging products.
Background technology
Dimethyl sulfoxide, molecular formula are (CH3)
2SO (writing a Chinese character in simplified form DMSO) is a kind of transparent, colourless, odorless, be the liquid of little bitterness, and toxicity is extremely low.It is a kind of water miscible chemical compound, can dissolve most organic compound, even also can dissolve inorganic salt.Liquid dimethyl sulfoxide can highly associate, and belongs to that polar solvent has very strong water absorption and to the permeability of human body.Dimethyl sulfoxide is mainly used organic synthesis solvents such as used as pesticides, dyestuff, medicine intermediate; Aromatic hydrocarbons extracting, synthetic fibre spinning high molecular polymerization, paraffin exquisiteness, diesel oil exquisiteness, electronic component cleaning, GAS ABSORPTION, rare metal extractant; Condenser dielectric antifreezing agent, hydraulic fluid raw material; The chemical reaction accelerated solvent; Pesticide, the fertile medicine of farming are imitated agent.Dimethyl sulfoxide itself has the effect of antiinflammatory, pain relieving, calmness, diuresis, promotion wound healing; And can will be dissolved in wherein other medicines smear by skin and infiltrate in the body, replaces oral or injects, be used for the treatment of arthritis, dermatitis, beriberi, psoriasis, sprain, various diseases such as tumor.So gain the name " catholicon " or " Dimethyl Sulfoxide ".But the research to its delaying senility function does not still have report.
Caenorhabditis elegans (Caenorhabditis elegans, be called for short nematicide) be a kind of simple relatively multicellular organism, hermaphroditism, be the model organism (H.Gershon of the old and feeble brilliance of research, D.Gershon.Caenorhabditis elegans-a paradigm for aging research:advantages and limitations.Mechanisms ofAgeing and Development 123 (2002) 261-274), compare with other species and to have the following advantage that hardly matches: I. easily cultivates, cheapness; II. the generation cycle weak point is fit to quick observation experiment result; III. offspring's number is many, is fit to statistical analysis; IV. molecular genetic techniques maturation; V. cell lineage knows that the VI. health is transparent, can very clearly examine under a microscope the structure of live body; VII. also be most important, nematicide and people are conservative aspect gene and molecular pathway, and such as the apoptosis path of at first finding in nematicide, the RNAi path is conservative in higher organism.VIII. be fit on a large scale the high flux screening medicine.IX. many hereditary paths are clear relatively such as researchs such as old and feeble associated class insulin path, limit food paths in the nematicide.
Not only can further excavate the value of existing medicine based on the medicine of this model organism exploitation slow down aging of nematicide, and can also contribute prolonging the human life-span.
Summary of the invention
The new purposes that the purpose of this invention is to provide dimethyl sulfoxide (DMSO).
A new purposes of dimethyl sulfoxide provided by the present invention is its application in the preparation anti-aging products.
Described product can be medicine, health product or food additive etc.
The new purposes of another of dimethyl sulfoxide provided by the present invention is its application in preparation nematicide life extender.Wherein, described nematicide specifically can be Caenorhabditis elegans (Caenorhabditis elegans).
When needing, in the said goods, can also add one or more pharmaceutically acceptable carriers.Described carrier comprises diluent, excipient, filler, binding agent, wetting agent, disintegrating agent, absorption enhancer, surfactant, absorption carrier, lubricant of pharmaceutical field routine etc.
Slow down aging medicine, health product with the dimethyl sulfoxide preparation can be made various ways such as injection, tablet, powder, granule, capsule, oral liquid, unguentum, cream.The medicine of above-mentioned various dosage forms all can be according to the conventional method preparation of pharmaceutical field.
The present invention adopts this model organism of nematicide, test as detection means with whole animal, observe dimethyl sulfoxide to the influence in nematicide life-span, find that volumn concentration is average life and the maximum life that 0.5% and 2% dimethyl sulfoxide can the significant prolongation nematicide.
Description of drawings
Fig. 1 is the bar diagram of the DMSO of variable concentrations to the influence of nematicide average life.
Fig. 2 is the nematicide survival curve figure of embodiment 1 preparation.
The specific embodiment
Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and biomaterial if no special instructions, all can obtain from commercial channels.
1 material
1.1 bacterial strain, nematicide
Bacterial strain E.coli OP50, wild type Caenorhabditis elegans Bristol (N
2) all available from nematicide heredity center (CGC).
1.2 medicine and reagent
DMSO (Sigma), agar powder (Nippon Union K. K), 5-fluorouracil (5-Fluorouracil, 5-Fu) (Sigma), peptone (peptone), tryptone (OXOID), all the other reagent are homemade.
1.3 instrument
MIR-553 constant incubator (Japanese SANYO company), S6E stereomicroscope (German Leica company), XTS30-D stereomicroscope (Tyke, Beijing Instr Ltd.), MLS-3780 high-pressure sterilizing pot (Japanese SANYO company) etc.
2 methods
2.1 experiment grouping
Be made as matched group (5-Fu group) and 6 DMSO medication groups, the concentration (volumn concentration) of DMSO in culture medium is followed successively by from low to high: 0.01%, 0.05%, 0.2%, 0.5%, 2%, 5%.
2.2 the routine of nematicide is cultivated
Hermaphroditism nematicide N2, (nematodegrowth medium is NGM) in 20 ℃ of constant temperature culture to be inoculated in the standard nematode culture medium that is coated with E.Coli OP50.Every liter of culture medium contains 3g sodium chloride, 20g agar powder, 2.5g peptone, 1ml cholesterol alcoholic solution (concentration is 5mg/ml), 1ml CaCl
2Solution (1mol/L), 1ml MgSO
4Solution (1mol/L) and 25ml KPO
4Solution (pH=6).
2.3 life experiment
(every liter of M9 solution contains Na 2.3.1 synchronization is with M9 buffer solution
2HPO
45.8g, KH
2PO
43.0g, NaCl 0.5g, NH
4Cl 1.0g) young adult is washed till in the aseptic EP pipe, add an amount of lysate (0.5% sodium hypochlorite, 0.5N cracking nematicide sodium hydroxide), put that centrifugal on the low speed centrifuge (3000rpm 1min), abandons supernatant, reuse M9 buffer solution flushing nematicide 2 times, centrifugal abandon behind the supernatant drawn EP pipe bottom nematicide with liquid-transfering gun and dripped aseptic area in NGM, and the intravital germ cell of cracked nematicide develops into L4 phase larva substantially after about 48 hours, finishes synchronization and is used for life experiment.
2.3.2 contain 12.5mg/L 5-Fu in the medium preparation matched group culture medium, contain the DMSO of 12.5mg/L 5-Fu and variable concentrations in the medicine group culture medium.
2.3.3 the synchronized L4 nematicide of life experiment picking every group average 80～110, has 15 approximately in each plate in each group culture medium.Constantly begin to calculate nematicide survival natural law from shifting, shift and be designated as life experiment the 0th day (day 0) same day.Check nematicide every day, record verge of death borer population is rejected unexpected death, escape and capsule line-transect worm.The dead criterion of nematicide: touch polypide with platinum filament, do not have any reaction, promptly be judged as death.More than experiment all repeats twice.
2.4 statistical analysis
Adopt the ANOVA variance test to analyze the life experiment result, quantitative data is represented with X ± S.
3 results
Variable concentrations DMSO the results are shown in Table 1 to the influence in nematicide life-span.As shown in Table 1, compare 0.5% and 2%DMSO significant prolongation nematicide average life and maximum life with matched group.
Table 1 variable concentrations DMSO is to the influence in nematicide life-span (X ± S)
Annotate: maximum life is the average life of the last existence 15% of colony; Compare with matched group,
1)P＜0.01,
2)P＜0.05
Variable concentrations DMSO is done bar diagram (get for the first time experimental result) to the result that influences of nematicide average life, see Fig. 1.Compare as can be seen 0.5% and 2%DMSO significant prolongation nematicide average life with matched group 5-Fu, 5%DMSO has shown that certain toxicity causes nematicide average life to shorten.
Use SPSS16.0 software that the omnidistance survival condition of nematicide is done survival curve (the Kaplan-Meier survival curve is got experimental result for the first time) (see figure 2), as seen from the figure 0.5% and 2%DMSO the survival curve of nematicide is moved to right, show the effect of lengthening the life.
Claims (4)
1. the application of dimethyl sulfoxide in the preparation anti-aging products.
2. application according to claim 1 is characterized in that: described product is medicine, health product or food additive.
3. the application of dimethyl sulfoxide in preparation nematicide life extender.
4. application according to claim 3 is characterized in that: described nematicide is a Caenorhabditis elegans.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102440382A CN101732291B (en) | 2009-12-25 | 2009-12-25 | Application of dimethyl sulfoxide in preparing anti-aging products |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102440382A CN101732291B (en) | 2009-12-25 | 2009-12-25 | Application of dimethyl sulfoxide in preparing anti-aging products |
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| CN101732291A true CN101732291A (en) | 2010-06-16 |
| CN101732291B CN101732291B (en) | 2011-11-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309387A (en) * | 2014-06-04 | 2016-02-10 | 兰州红菌生物技术有限责任公司 | Kit for screening anti-aging drugs and using method thereof |
| CN108849771A (en) * | 2018-08-20 | 2018-11-23 | 扬州大学 | Peony bud is in the application extended in the nematode service life |
-
2009
- 2009-12-25 CN CN2009102440382A patent/CN101732291B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309387A (en) * | 2014-06-04 | 2016-02-10 | 兰州红菌生物技术有限责任公司 | Kit for screening anti-aging drugs and using method thereof |
| CN108849771A (en) * | 2018-08-20 | 2018-11-23 | 扬州大学 | Peony bud is in the application extended in the nematode service life |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101732291B (en) | 2011-11-23 |
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