CN101718692B - Method for quickly detecting content of fat, protein and dry substances in bovine coloctrum - Google Patents
Method for quickly detecting content of fat, protein and dry substances in bovine coloctrum Download PDFInfo
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- CN101718692B CN101718692B CN 200910260127 CN200910260127A CN101718692B CN 101718692 B CN101718692 B CN 101718692B CN 200910260127 CN200910260127 CN 200910260127 CN 200910260127 A CN200910260127 A CN 200910260127A CN 101718692 B CN101718692 B CN 101718692B
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Abstract
The invention relates to the field of dairy products, in particular to a method for quickly detecting the content of fat, protein and dry substances in bovine coloctrum. The quick detection method of the invention comprises the following steps of: 1) diluting a bovine coloctrum sample with distilled water, wherein the dilution proportion for detecting the fat content is that the ratio of the bovine coloctrum to water is 3 to 5, the dilution proportion for detecting the protein content is that the ratio of the bovine coloctrum to the water is 3 to 10, and the dilution proportion for detecting the dry substance content is that the ratio of the bovine coloctrum to the water is 3 to 5; 2) detecting the sample by using an Fourier infrared full-spectrum scanning dairy component analyzer; and 3) multiplying an obtained detection result by the corresponding dilution ratio in the step 1). The method overcomes the disadvantages of long time and complicated steps of routine detection, can accurately determine the content of main indexes in the bovine coloctrum, provides a quick, simple and convenient method for factory acquisition of the bovine coloctrum, and is applied in the fields of quick acquisition and analytic detection of products.
Description
Technical field
The present invention relates to field of dairy products, particularly, the present invention relates to the method for fat, protein, dry matter content in a kind of fast detecting colostrum.
Background technology
Colostrum is a few days ago a milk after the milk cow calving, because of containing abundant bioactivator, has very high researching value in the colostrum, and these active components make colostrum have the gastrointestinal function of improvement; Infecting of opposing pathogenic entero becteria; The systemic immunity ability of enhancing body; Regulate the body physiological state balance; Quicken special physiological properties such as healthy rejuvenation.Therefore the people's attention extremely of the research of colostrum in the last few years.
In the checkout procedure of colostrum purchase, check fat adopts method in the vigorous method of lid, Lodz-Gothic usually, cling to not cock method etc.; Detect protein and often adopt Kjeldahl; Dry often adopts the anti-method that pushes away solid content of water evaporates, and adopting these national standard methods commonly used to detect at least needs several hours, and complicated steps, is not suitable for plant produced and purchases fast.At present, fourier infrared is composed scanning dairy component analyser entirely and in fast detecting such as the protein content of analyzing normal breast, fat content, dry matter content, is used very extensively, and this method national standard method commonly used is easy, and efficient is high.But because of indexs such as serum colostrum protein, fat are different with normal breast, it is worth not in the range of linearity of dairy component analyser analytic curve, and its viscosity is high, can not directly measure.For this reason, we have explored and have adopted fourier infrared to compose the method that scanning dairy component analyser detects the conventional physical and chemical index of colostrum entirely, to difference index to be measured, use the different disposal method to measure.
Fourier infrared is composed scanning dairy component analyser entirely and is adopted the full spectral transmission technology of fourier infrared; Accurate component is based on the measuring method of the infrared radiation of material absorbing specific wavelength in the analysis milk; Detect quick and precisely, detected a sample in 30～40 seconds, easy and simple to handle; Be easy to safeguard, be widely used in the control detection of the raw material cow's milk purchase detection of big-and-middle-sized Milk Processing Plant and production run and final products at present.
Summary of the invention
The method that the purpose of this invention is to provide fat, protein, dry matter content in a kind of fast detecting colostrum.
According to method for quick of the present invention, it may further comprise the steps:
1) the colostrum sample is used distilled water diluting, wherein,
The dilution ratio that detects fat content is a colostrum: water=3: 5,
The dilution ratio that detects protein content is a colostrum: water=3: 10,
The dilution ratio that detects dry matter content is a colostrum: water=3: 5;
2) composing scanning dairy component analyser entirely with fourier infrared detects sample; The index that this dairy component analyser setup parameter is selected " milk " test module and needed to detect obtains fat, the protein in the above-mentioned colostrum sample, the content results of dry;
3) above-mentioned gained testing result multiply by the corresponding extension rate in the step 1), promptly get the actual content of fat, protein and dry in the colostrum sample.
According to the method for the invention, wherein, said blend step 1) type of heating is the water-bath heating, and temperature is 40 ℃, will avoid long and overheated heating products during heating, generally about 10min, to prevent lipolysis.The mixing step adopts eddy mixer.Fourier infrared is composed scanning dairy component analyser detection method entirely: calibration sample curve as required before the measurement, the input reference data is calculated its adjustment, preserves adjustment.The index of selecting " milk " test module during measurement and needing to detect detects.
Inventor of the present invention is through to test the extension rate of colostrum when confirm detecting These parameters: adopt fourier infrared to compose scanning dairy component analyser entirely by the different proportion dilution colostrum and detect; Three measurements are averaged; And, draw best extension rate condition with national standard method and method for quick contrast.Testing result is as shown in table 1 below.
Table 1
By finding out in the table 1; Detecting the fat content dilution ratio is colostrum: water=3: 5; Detecting the protein content dilution ratio is colostrum: water=3: 10, detecting the dry matter content dilution ratio is colostrum: water=3: 5 o'clock, fast detecting value and GB detected value are the most approaching.And, the above-mentioned physics and chemistry value cross validation of the colostrum sample of the different acquisition time of inventor of the present invention through detecting same source and the colostrum sample of the identical acquisition time of separate sources the accuracy of method of the present invention.
Method of the present invention has remedied the shortcoming of long, complicated steps of conventional sense method time; Can accurately measure the content of leading indicator in the colostrum; For the purchase of the factory of colostrum provides a fast and convenient method, be applied to product fast in purchase and the analyzing and testing field.
Description of drawings
Fig. 1 is the fiducial interval figure according to the testing result of embodiments of the invention 1, and wherein, Fig. 1-1 is a fat content detected value probability graph, and Fig. 1-2 is fatty difference probability graph; Fig. 1-3 is a protein content detected value probability graph, and Fig. 1-4 is a protein difference probability graph; Fig. 1-5 is a dry detected value probability graph, and Fig. 1-6 is a dry difference probability graph.
Fig. 2 is the fiducial interval figure according to the testing result of embodiments of the invention 2, and wherein, Fig. 2-1 is a fat content detected value probability graph, and Fig. 2-2 is fatty difference probability graph; Fig. 2-3 is a protein content detected value probability graph, and Fig. 2-4 is a protein difference probability graph; Fig. 2-5 is a dry detected value probability graph, and Fig. 2-6 is a dry difference probability graph.
Fig. 3 is the fiducial interval figure according to the testing result of embodiments of the invention 3, and wherein, Fig. 3-1 is a fat content detected value probability graph, and Fig. 3-2 is fatty difference probability graph; Fig. 3-3 is a protein content detected value probability graph, and Fig. 3-4 is a protein difference probability graph; Fig. 3-5 is a dry detected value probability graph, and Fig. 3-6 is a dry difference probability graph.
Embodiment
Below specify technology of the present invention and characteristics through specific embodiment, but these embodiment are not in order to limit protection scope of the present invention.
The physical and chemical index of embodiment 1, fast detecting colostrum
The colostrum sample is used distilled water diluting, and detecting the fat content dilution ratio is colostrum: water=3: 5, detecting the protein content dilution ratio is colostrum: water=3: 10, detecting the dry matter content dilution ratio is colostrum: water=3: 5.Water-bath is heated to 40 ℃, with vortex oscillation device mixing.Fourier infrared is composed the index that scanning dairy component analyser parameter is selected " milk " test module and needed to detect entirely.Adopt fourier infrared to compose scanning dairy component analyser full sample is carried out three parallel detections; Institute is surveyed the result multiply by corresponding extension rate; Promptly get the actual content of this material; And the fourier infrared of colostrum sample is composed scanning dairy component analyser testing result entirely compare its result such as following table 2 with the national standard detection method.
Through carrying out parallel detection with the method, the repeated testing result of same sample meets the tolerance of method, the good reproducibility of method, and the accuracy of this method for quickly detecting is higher.
Table 2
Detect 10 samples that the source is identical, acquisition time is identical with said method, the result is as shown in table 3 below.
Table 3
Validation of Data:
Utilize the MINITAB statistical analysis software, will be 95 fiducial interval figure respectively with the fast detecting value of machine testing fat, protein, dry and the difference of fast detecting value and GB detected value, shown in Fig. 1-1～Fig. 1-6.The difference of fast method detected value and GB detected value of fast method detected value and each material that can find out fat, protein, dry from fiducial interval figure explains that the data error that obtains is an acceptable all in 95% fiducial interval.
The physical and chemical index of embodiment 2, fast detecting colostrum
Use method of the present invention and national standard method to detect respectively from physical and chemical index same pasture, acquisition time (1～3 day) different samples (1～3 day).
The colostrum sample is used distilled water diluting, and detecting the fat content dilution ratio is colostrum: water=3: 5, detecting the protein content dilution ratio is colostrum: water=3: 10, detecting the dry matter content dilution ratio is colostrum: water=3: 5.Water-bath is heated to 40 ℃, with vortex oscillation device mixing.Fourier infrared is composed the index that scanning dairy component analyser parameter is selected " milk " test module and needed to detect entirely.Adopt fourier infrared to compose scanning dairy component analyser full sample is carried out three parallel detections; Institute is surveyed the result multiply by corresponding extension rate; Promptly get the actual content of this material; And the fourier infrared of colostrum sample is composed scanning dairy component analyser testing result entirely compare its result such as following table 4 with the national standard detection method.
Table 4
Utilize the MINITAB statistical analysis software, will be 95 fiducial interval figure respectively with the fast detecting value of machine testing fat, protein, dry and the difference of fast detecting value and GB detected value, shown in Fig. 2-1～Fig. 2-6.The difference of fast method detected value and GB detected value of fast method detected value and each material that can find out fat, protein, dry from fiducial interval figure explains that the data error that obtains is an acceptable all in 95% fiducial interval.
The physical and chemical index of embodiment 3, fast detecting colostrum
Use method of the present invention and national standard method to detect to gather the colostrum that produced in second day in different pastures respectively, the fast method detected value of fat, protein, dry and the fast detecting value of each material and the difference of GB detected value are 95 fiducial interval figure respectively.The result is as shown in table 5 below.
Table 5
Utilize the MINITAB statistical analysis software, will be 95 fiducial interval figure respectively with the fast detecting value of machine testing fat, protein, dry and the difference of fast detecting value and GB detected value, shown in Fig. 3-1～Fig. 3-6.The difference of fast method detected value and GB detected value of fast method detected value and each material that can find out fat, protein, dry from fiducial interval figure explains that the data error that obtains is an acceptable all in 95% fiducial interval.
Claims (2)
1. the method for fat, protein, dry matter content in the fast detecting colostrum is characterized in that said method comprises the steps:
1) the colostrum sample is used distilled water diluting, wherein,
The dilution ratio that detects fat content is a colostrum: water=3: 5,
The dilution ratio that detects protein content is a colostrum: water=3: 10,
The dilution ratio that detects dry matter content is a colostrum: water=3: 5;
2) compose scanning dairy component analyser entirely with fourier infrared sample is detected, obtain fat, the protein in the above-mentioned colostrum sample, the content results of dry;
3) above-mentioned gained testing result multiply by the corresponding extension rate in the step 1), promptly get the actual content of fat, protein and dry in the colostrum sample.
2. detection method according to claim 1 is characterized in that, in said step 1), colostrum mixes under 40 ℃ with distilled water, and incorporation time is 10min.
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| CN102095698A (en) * | 2010-12-08 | 2011-06-15 | 内蒙古伊利实业集团股份有限公司 | Method for rapidly detecting physical and chemical indexes of colostrum by near infrared spectroscopy |
| CN102288329B (en) * | 2011-07-04 | 2012-11-21 | 广西壮族自治区水牛研究所 | Method for quickly determining total heat of raw milk of water buffalo |
| CN105301209A (en) * | 2015-06-30 | 2016-02-03 | 南京卫岗乳业有限公司 | Detection method for protein content in grain milk |
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