CN101716192B - Method for separating pharmacodynamic substances of Chinese medicinal mole crickets by utilizing high-speed countercurrent chromatography - Google Patents
Method for separating pharmacodynamic substances of Chinese medicinal mole crickets by utilizing high-speed countercurrent chromatography Download PDFInfo
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- CN101716192B CN101716192B CN2010103001031A CN201010300103A CN101716192B CN 101716192 B CN101716192 B CN 101716192B CN 2010103001031 A CN2010103001031 A CN 2010103001031A CN 201010300103 A CN201010300103 A CN 201010300103A CN 101716192 B CN101716192 B CN 101716192B
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- gryllotalpa
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- ethyl acetate
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Abstract
The invention provides a method for separating pharmacodynamic substances of Chinese medicinal mole crickets by utilizing high-speed countercurrent chromatography, which belongs to technology for extracting pharmacodynamic substances of mole crickets. Steps of the method include material selection, crushing, crude extraction, concentration, countercurrent chromatography separation, collection and drying. The method utilizes the high-speed countercurrent chromatography to rapidly and efficiently separate and extract anti-cancer pharmacodynamic substances in the Chinese medicinal mole crickets, is time-saving, economical, rapid and efficient compared with conventional chromatography extraction methods, and provides a basis for the further research and development of novel medicaments.
Description
Technical field
The invention belongs to Gryllotalpa effective substance extractive technique, be specifically related to relate to a kind of method of utilizing high speed adverse current chromatogram separation and Extraction effective substance from Chinese medicine, be i.e. the method for distilling of anticarcinogen effective substance in the Gryllotalpa.
Background technology
Gryllotalpa is Insecta (Insecta), Orthoptera (Orthoptera), Gryllotalpidae (Gryllotalpide); Gryllotalpa spp (Gryllotalpa) animal; Kind surplus Gryllotalpa spp has 30, China produces 6 kinds, and wherein east Gryllotalpa G.orientalis and Gryllotalpa unispina G.unipina are medicinal kind.Gryllotalpa property of medicine salty in the mouth, cold in nature, return bladder, small intestinal, large intestine channel.Treatment sphincter disturbance, all kinds of edema, cirrhosis ascites disease, the treatment of treatment urine retention, urinary system calculus, miscellaneous diseases such as treatment carbuncle, scrofula, malignant boil, we are clinical to find that with experiment the Gryllotalpa ethanol extraction has tangible cytotoxicity to HCC.
High speed adverse current chromatogram (high speed countercurrentchromatography; Be called for short HSCCC) be a kind of liquid-liquid chromatography isolation technics; Its immobile phase and mobile phase all are liquid, owing to do not need solid support, do not have Irreversible Adsorption; Compare with traditional isolation and purification method, have sample free of losses, pollution-free, efficient, quick and big preparation amount and advantage such as separate.Utilize high speed adverse current chromatogram that sample can all be reclaimed; The sample that reclaims more can reflect the characteristic that it is original; Be particularly suitable for the separation of natural bioactive ingredients; Be a kind of ideal big preparation amount separation means, thus utilize high speed adverse current chromatogram to prepare pharmacodynamics material in the Gryllotalpa to have fast, effect efficiently and easily.
Summary of the invention
The objective of the invention is to utilize high speed adverse current chromatogram, fast, the anticarcinogen effective substance in the high efficiency separation, extraction Chinese medicine Gryllotalpa, than traditional chromatograph mention method save time, economical, fast, efficient, for further new drug research exploitation provides the basis.
The objective of the invention is to realize through following method.
Comprise material select, broken, slightly carry, concentrate, adverse current chromatogram separates, collect and drying, concrete steps comprise:
(1) Gryllotalpa grinds;
(2) adopt 70% ethanol water (V/V), Gryllotalpa and 70% ethanol water (V/V) are than being: 1/3 (W/W), 80 ℃ of reflux, extract, 3 times; Merge extractive liquid; (model: RE-5210), temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no pure extractum under the 0.8pa at vacuum rotation concentrating instrument;
(3) the nothing alcohol extractum that obtains of said step (2) adds petroleum ether extraction 3 times, and consumption is according to extractum: petroleum ether volume ratio=1: 2;
(4) extractum adds ethyl acetate extraction 3 times behind step (3) petroleum ether extraction, collects ethyl acetate extraction liquid; Consumption is according to preparatory extraction extractum: ethyl acetate volume ratio=1: 2;
(5) ethyl acetate extraction liquid is put in the vacuum rotation concentrating instrument, and temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no ethyl acetate extractum under the 0.8pa;
(6) extractum that obtains in the step (5) adopts the high speed adverse current chromatogram separation, and (model: TBE-200V), solvent system is the mixed solution of normal hexane, second alcohol and water, in this mixed solution: normal hexane: ethanol: water (V/V)=6: 5: 1; Detached dowel volume 230ml; Mobile phase is last phase, rotating speed 1000r/min, flow velocity 1.5mL/min; Sample size 400mg, immobile phase keeps 48%, detects wavelength 245nm;
(7) go out kind time and rise, 30-35min collects sample separation;
(8) collect sample, in vacuum rotation concentrating instrument (RE-5210), temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into solvent-freely under the 0.8pa, promptly obtains the Gryllotalpa effective substance.
Gryllotalpa of the present invention is adopted east Gryllotalpa G.orientalis or Gryllotalpa unispina G.unipina.
Remarkable advantage of the present invention is: method of the present invention saves time, economy, quick, efficient, and separate than tradition and reduce the use solvent more than 90%, 10 times of disengaging time shortenings, yield improves more than 50%, and material enrichment concentration improves 2 times.
The specific embodiment
In order fully to disclose the separation method of Chinese medicine Gryllotalpa pharmacodynamics material of the present invention, explain below in conjunction with embodiment.
Embodiment 1: the method for utilizing high performance countercurrent chromatography separating traditional Chinese medicine Gryllotalpa pharmacodynamics material
Utilize the method for high performance countercurrent chromatography separating traditional Chinese medicine Gryllotalpa pharmacodynamics material, may further comprise the steps:
(1) Gryllotalpa grinds;
(2) adopt 70% ethanol water (V/V), Gryllotalpa and 70% ethanol water (V/V) are than being: 1/3 (W/W), 80 ℃ of reflux, extract, 3 times; Merge extractive liquid; (model: RE-5210), temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no pure extractum under the 0.8pa at vacuum rotation concentrating instrument;
(3) the nothing alcohol extractum that obtains of said step (2) adds petroleum ether extraction 3 times, and consumption is according to extractum: petroleum ether volume ratio=1: 2;
(4) extractum adds ethyl acetate extraction 3 times behind step (3) petroleum ether extraction, collects ethyl acetate extraction liquid; Consumption is according to preparatory extraction extractum: ethyl acetate volume ratio=1: 2;
(5) ethyl acetate extraction liquid is put in the vacuum rotation concentrating instrument, and temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no ethyl acetate extractum under the 0.8pa;
(6) extractum that obtains in the step (5) adopts the high speed adverse current chromatogram separation, and (model: TBE-200V), solvent system is the mixed solution of normal hexane, second alcohol and water, in this mixed solution: normal hexane: ethanol: water (V/V)=6: 5: 1; Detached dowel volume 230ml; Mobile phase is last phase, rotating speed 1000r/min, flow velocity 1.5mL/min; Sample size 400mg, immobile phase keeps 48%, detects wavelength 245nm;
(7) go out kind time and rise, 30-35min collects sample separation;
(8) collect sample, in vacuum rotation concentrating instrument (RE-5210), temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into solvent-freely under the 0.8pa, promptly obtains the Gryllotalpa effective substance.
Embodiment 2:
The dry product of getting Gryllotalpa unispina G.unipina is a raw material, presses the method for embodiment 1 and extracts the pharmacodynamics material.
The present invention reduces the use solvent more than 90% than traditional the separation, and disengaging time shortens more than 10-12 times, and yield improves more than the 50-55%, and material enrichment concentration improves 2-2.5 times.
The effective substance that the present invention extracts belongs to lactone.With the QGY7703 HCC is experiment material, adopts crystal violet staining assay.The every hole of 96 porocyte culture plates adds QGY7703 cell (1 * 10
4Individual), at 37 ℃ of CO
2Hatch 24h in the incubator.Add medicine (the amount 1.6 μ g/ml that add medicine) after 48 hours, violet staining liquid dyeing 0.5h, the washing back adds crystal violet extracting solution 100ul, and mixing is measured trap value A with the enzyme-linked immunoassay appearance then
595By formula: cell toxicant %=A
Solvent-A
Medicine/ A
Solvent* 100, calculate cell toxicant %.Cytotoxic activity is higher than negative control 30.47%.
Effective dose when Gryllotalpa effective substance of the present invention is used for medical usage is: 10-30mg/10g; Can be certain medicine material according to effective dose with Gryllotalpa effective substance of the present invention; Compatibility and other can allow the Chinese medicine or the chemicals that share again, are prepared into various dosage forms or the like.
Claims (2)
1. method of utilizing high speed adverse current chromatogram separating traditional Chinese medicine Gryllotalpa pharmacodynamics material is characterized in that: comprise material select, broken, slightly carry, concentrate, adverse current chromatogram separates, collect and drying, concrete steps comprise:
(1) Gryllotalpa grinds;
(2) adopting volume fraction is 70% alcoholic solution, and the mass ratio of Gryllotalpa and 70% alcoholic solution is 1: 3,80 ℃ of reflux, extract, 3 times; Merge extractive liquid; In vacuum rotation concentrating instrument, temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no pure extractum under the 0.8Pa;
(3) the nothing alcohol extractum that obtains of said step (2) adds petroleum ether extraction 3 times, and consumption is according to extractum: petroleum ether volume ratio=1:2;
(4) extractum adds ethyl acetate extraction 3 times behind step (3) petroleum ether extraction, collects ethyl acetate extraction liquid; Consumption is according to preparatory extraction extractum: ethyl acetate volume ratio=1:2;
(5) ethyl acetate extraction liquid is put in the vacuum rotation concentrating instrument, and temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into no ethyl acetate extractum under the 0.8Pa;
(6) extractum that obtains in the step (5) adopts high speed adverse current chromatogram to separate, and solvent system is the mixed solution of normal hexane, second alcohol and water, in this mixed solution: normal hexane: ethanol: the volume ratio=6:5:1 of water; Detached dowel volume 230ml; Mobile phase is last phase, rotating speed 1000r/min, flow velocity 1.5mL/min; Sample size 400mg, immobile phase keeps 48%, detects wavelength 245nm;
(7) go out kind time and rise, 30-35min collects sample separation;
(8) collect sample, in vacuum rotation concentrating instrument, temperature is 60 ℃, and pressure is that the vacuum rotation is concentrated into solvent-freely under the 0.8Pa, promptly obtains the Gryllotalpa effective substance.
2. the method for utilizing high speed adverse current chromatogram separating traditional Chinese medicine Gryllotalpa pharmacodynamics material according to claim 1 is characterized in that: described Gryllotalpa is adopted the east Gryllotalpa
G. orientalisOr Gryllotalpa unispina
G. unipina
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010103001031A CN101716192B (en) | 2010-01-07 | 2010-01-07 | Method for separating pharmacodynamic substances of Chinese medicinal mole crickets by utilizing high-speed countercurrent chromatography |
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| CN2010103001031A CN101716192B (en) | 2010-01-07 | 2010-01-07 | Method for separating pharmacodynamic substances of Chinese medicinal mole crickets by utilizing high-speed countercurrent chromatography |
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| CN101716192B true CN101716192B (en) | 2012-03-21 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104003923B (en) * | 2014-05-30 | 2016-06-15 | 河南科技大学 | The application of the extracting method of alkaloid compound and this compound in Gryllotalpa |
| CN109549956B (en) * | 2017-09-25 | 2022-07-12 | 上海医药工业研究院 | Application of mole cricket extract in preparing medicament for treating brain glioma |
| CN109568347B (en) * | 2017-09-25 | 2022-07-12 | 上海医药工业研究院 | Gryllotalpa extract and its extraction method |
| CN109589340B (en) * | 2017-09-29 | 2022-07-12 | 上海医药工业研究院 | Application of mole cricket extract in preparing medicine for treating breast cancer with three yin |
| CN109589341B (en) * | 2017-09-29 | 2022-07-12 | 上海医药工业研究院 | Gryllotalpa extract and its preparation method |
Citations (2)
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| CN1582970A (en) * | 2004-06-15 | 2005-02-23 | 上海澳隆科技发展有限公司 | Extraction of effective portion of gryllotalpidae for anti-cancers and its preparation |
| CN101250102A (en) * | 2008-03-31 | 2008-08-27 | 福建农林大学 | Method for extracting phenylacetic acid substance from gryllotalpidae |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582970A (en) * | 2004-06-15 | 2005-02-23 | 上海澳隆科技发展有限公司 | Extraction of effective portion of gryllotalpidae for anti-cancers and its preparation |
| CN101250102A (en) * | 2008-03-31 | 2008-08-27 | 福建农林大学 | Method for extracting phenylacetic acid substance from gryllotalpidae |
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| 王祖林等.高速逆流色谱在天然产物有效成分分离中的应用.《山东科学》.2009,第22卷(第3期),40-44. * |
| 郭澄等.超临界萃取蝼蛄脂肪酸成分及其气相色谱-质谱分析.《分析化学》.2006,第34卷(第S1期),15-18. * |
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