CN101712013B - Method for classifying calcium alginate plastic beads - Google Patents
Method for classifying calcium alginate plastic beads Download PDFInfo
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- CN101712013B CN101712013B CN 200810013524 CN200810013524A CN101712013B CN 101712013 B CN101712013 B CN 101712013B CN 200810013524 CN200810013524 CN 200810013524 CN 200810013524 A CN200810013524 A CN 200810013524A CN 101712013 B CN101712013 B CN 101712013B
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Abstract
The invention relates to a method for classifying calcium alginate plastic beads. In the method, the plastic beads to be classified are put into a fluidized bed and are used as solid phases; solution of calcium chloride is used as a mobile phase; the solution of the calcium chloride flows into the fluidized bed from a fluid inlet on the lower part of the fluidized bed through a peristaltic pump, flows through the inside of the fluidized bed, and finally flows out of the fluidized bed from a fluid outlet on the upper part of the fluidized bed; and the plastic beads are classified by changing the flow velocity of the ascending fluid according to the different sedimentation velocities of the plastic beads with different grain sizes in the fluid. The method classifies the calcium alginate plastic beads according to the grain sizes, the used device is simple, is easy to amplify and can separate the plastic beads of which the average grain size difference is 200 microns, and the classification range is between 100 and 1,000 microns.
Description
Technical field
The present invention relates to the classification of gel-like pearl microballoon, specifically utilize liquid-solid fluidized bed inner fluid mechanics principle, according to particle size it is carried out the method for classification.
Background technology
Calcium alginate plastic beads (hereinafter to be referred as the glue pearl) belongs to a kind of of gel-like microballoon, and density is 1.02-1.03g/cm
3, the field such as discharge in microcapsules preparations, medicine control, transplant in the immobilization of cell, body is studied widely and is used.The particle size range of the glue pearl of static titration preparation is 200-1000 μ m, and in order to obtain the comparatively glue pearl of homogeneous of particle diameter, the normal stage division that adopts is the screen cloth screening, and its classification efficiency is low, results in blockage easily.The human hairs such as Zhang Qizhou understand a kind of Beaded agarose gel screening grader (patent publication No. CN2043135), and this device has improved classification efficiency on the basis of screen cloth screening, but the classification of the method still is subject to the restriction of screen cloth.
Summary of the invention
The object of the present invention is to provide a kind of new method of classification calcium alginate plastic beads, the party's ratio juris is to utilize the fluidization characteristics of glue pearl in fluid bed to realize classification, according to the difference of glue pearl sinking speed in fluid of different-grain diameter, make the glue pearl obtain classification (seeing accompanying drawing 5) by the flow velocity that changes ascending fluid.The method device therefor is simple, is not subjected to the restriction of the used mesh size of traditional classification, can realize series classification, and the glue pearl that the particle diameter minimum can be differed about 200 μ m carries out classification, and this stage division amplifies the classification efficiency height easily.
For achieving the above object, the technical solution used in the present invention is:
A kind of method of classifying calcium alginate plastic beads, glue pearl to be fractionated is placed in the fluid bed, the conduct of glue pearl is phase fixedly, calcium chloride solution is as mobile phase, calcium chloride solution flows into by the lower flow entrance of peristaltic pump from fluid bed, and is inner through fluid bed, flows out from the upper flow exit at last, according to the difference of glue pearl sinking speed in fluid of different-grain diameter, make the glue pearl obtain classification by the flow velocity that changes ascending fluid;
Described fluid bed is the column type fluid bed, ratio of height to diameter is 5-20, there is the lower end to be provided with on the sidewall that fluid intake, a upper end is provided with a fluid issuing, its middle and upper part and is provided with a material outlet, material outlet is positioned at the 2/3-5/6 of fluidized bed height, and the below, inside of fluid bed is provided with fluid distribution plate.
Its specific operation process is as follows,
1) glue pearl to be fractionated is placed in the fluid bed, the volume of glue pearl is fluid bed volume 1/10-2/5.
2) open peristaltic pump, the fluid intake of calcium chloride solution from the fluid bed lower end slowly pumped into, open simultaneously the upper end fluid issuing of fluid bed, the surface velocity of fluid is controlled at 0.01-0.1cm/s, until fluid is full of the inner chamber of whole fluid bed;
3) rotating speed of increase peristaltic pump, to improve the flow velocity of fluid, until the bed expansion height of glue pearl is when consistent with the material outlet height on the fluid bed sidewall, open material outlet, close simultaneously the upper end fluid issuing, keep stablizing 5-30 minute under the constant flow rate, the glue pearl of flowing out fluid bed is first glue pearl that is graded;
4) if also will continue the glue pearl of staying in the fluid bed is carried out classification, just continue at again and increase rate of flow of fluid when consistent with the material outlet height on the fluid bed sidewall to the bed expansion height of glue pearl, keep stablizing 5-30 minute under the constant flow rate, the glue pearl of flowing out fluid bed is the glue pearl that second batch is graded; Carry out successively aforesaid operations, can obtain the calcium alginate plastic beads of different-grain diameter five equilibrium.
The concentration of described calcium chloride solution is 0.05-0.3mol/L.The surface velocity of fluid is generally 0.1-0.6cm/s during classification.
The present invention has following advantage:
1. equipment is simple, easy operating.Be not subjected to the restriction of the used screen cloth of traditional classification, Gu employed plant equipment only has a kind of fluid bed of liquid-type, only need just can realize the classification of glue pearl by the flow velocity of adjusting fluid.
2. compare with screen cloth screening, this stage division can be to glue pearl injury, and good separating effect.
3. can realize series classification, the glue pearl that the particle diameter minimum can be differed about 200 μ m carries out classification, and this stage division amplifies the classification efficiency height easily.
In a word, compare with traditional screen cloth screening, equipment is simple, easy to operate, the advantage of the high scale application of classification efficiency but the inventive method has.
Description of drawings
Fig. 1 is the particle diameter distribution map of glue pearl before and after embodiment 1 classification;
Fig. 2 is the particle diameter distribution map of glue pearl before and after embodiment 2 classifications;
Fig. 3 is the particle diameter distribution map of glue pearl before and after embodiment 3 classifications;
Fig. 4 is the schematic diagram of fluid bed of the present invention;
Fig. 5 be the flow velocity of fluid on different-grain diameter glue pearl in the dynamic (dynamical) impact of fluid bed inner fluid.
The specific embodiment
As shown in Figure 4, fluid bed of the present invention is the column type fluid bed, ratio of height to diameter is 5-20, there is the lower end to be provided with on the sidewall that fluid intake 1, a upper end is provided with a fluid issuing 2, its middle and upper part and is provided with a material outlet 3, material outlet is positioned at the 2/3-5/6 of fluidized bed height, and the below, inside of fluid bed is provided with fluid distribution plate 4.
The present invention is according to fluid mechanics principle, in fluid bed, realize the classification to the glue pearl, and the fluidization characteristic of glue pearl in fluid bed depends on size, shape and the density of particle and the flow velocity of fluid, in the certain situation of rear three factors, the fluidization characteristic of glue pearl depends primarily on the size of its particle diameter.As shown in Figure 5, the expansion height of glue pearl in fluid bed of different-grain diameter.
The method device therefor is simple, amplifies easily, and the glue pearl that average grain diameter can be differed 200 microns is separated, and grading range is at the 100-1000 micron.
1) particle diameter on particle size analyzer detection glue pearl to be fractionated distributes (seeing the blue distributing line among Fig. 1), and the particle size range of this glue pearl comprises two parts, the minimum grain size 185 μ m of first, and maximum particle diameter 270 μ m, average grain diameter is 225 ± 15 μ m; The minimum grain size 356 μ m of second portion, maximum particle diameter 471 μ m, average grain diameter is 412 ± 19 μ m.
2) glue pearl to be fractionated is placed in the fluid bed, the volume of glue pearl accounts for 1/5 of fluid bed volume.
3) open peristaltic pump, being the 0.05mol/L calcium chloride solution with concentration slowly pumps into from the fluid intake 1 of fluid bed, opens simultaneously the fluid issuing 2 of fluid bed, and the surface velocity of fluid is controlled at 0.05cm/s, until fluid is full of the inner chamber of whole fluid bed.
4) slowly increase the rotating speed of peristaltic pump, improving the flow velocity of fluid, until the bed expansion height of glue pearl is when consistent with the height of material outlet 3, open material outlet 3, close simultaneously fluid issuing 2, continue slowly to increase rate of flow of fluid to 0.10-0.12cm/s, kept flow speed stability 10 minutes.Treated, the glue pearl of first is flowed out fluid bed, the glue pearl of second portion still is retained in the fluid bed (seeing the red distributing line among Fig. 1), it is 419 ± 17 μ m that particle size analyzer detects the average grain diameter of staying glue pearl in the fluid bed, and the scope that particle diameter distributes is with to separate front second portion consistent, the result shows that the method can be separated the glue pearl of two parts different-grain diameter fully.
1) particle diameter on particle size analyzer detection glue pearl to be fractionated distributes (seeing the blue distributing line among Fig. 2), and the particle size range of this glue pearl comprises two parts, the minimum grain size 391 μ m of first, and maximum particle diameter 471 μ m, average grain diameter is 429 ± 20 μ m; The minimum grain size 517 μ m of second portion, maximum particle diameter 751 μ m, average grain diameter is 623 ± 39 μ m.
2) glue pearl to be fractionated is placed in the fluid bed, the volume of glue pearl accounts for 1/5 of fluid bed volume.
3) open peristaltic pump, being the 0.1mol/L calcium chloride solution with concentration slowly pumps into from the fluid intake 1 of fluid bed, opens simultaneously the fluid issuing 2 of fluid bed, and the surface velocity of fluid is controlled at 0.1cm/s, until fluid is full of the inner chamber of whole fluid bed.
4) slowly increase the rotating speed of peristaltic pump, to improve the flow velocity of fluid, until the bed expansion height of glue pearl is when consistent with the height of material outlet 3, open material outlet 3, close simultaneously fluid issuing 2, continue slowly to increase rate of flow of fluid to 0.15-0.18cm/s, kept flow speed stability 15 minutes, the glue pearl of flowing out fluid bed is first glue pearl (seeing the red square distributing line among Fig. 2) that is graded, average grain diameter is 451 ± 21 μ m, the glue pearl that rests in the fluid bed is the second batch glue pearl (seeing the red starlike distributing line among Fig. 2) that is graded, and average grain diameter is 649 ± 30 μ m.Although some variations have occured the particle size range of glue pearl after the classification, from the result, the method can be separated the glue pearl of two kinds of particle size range.
1) particle diameter on particle size analyzer detection glue pearl to be fractionated distributes (seeing the blue distributing line among Fig. 3), and the particle size distribution range of glue pearl is wider, minimum grain size 293 μ m, maximum particle diameter 815 μ m.
2) glue pearl to be fractionated is placed in the fluid bed, the volume of glue pearl accounts for 1/10 of fluid bed volume.
3) open peristaltic pump, being the 0.3mol/L calcium chloride solution with concentration slowly pumps into from the fluid intake 1 of fluid bed, opens simultaneously the fluid issuing 2 of fluid bed, and the surface velocity of fluid is controlled at 0.06cm/s, until fluid is full of the inner chamber of whole fluid bed.
4) slowly increase the rotating speed of peristaltic pump, to improve the flow velocity of fluid, until the bed expansion height of glue pearl is when consistent with the height of material outlet 3, open material outlet 3, close simultaneously fluid issuing 2, continue slowly to increase rate of flow of fluid to 0.15cm/s, kept flow speed stability 25 minutes, the glue pearl of flowing out fluid bed is first glue pearl (seeing the red square distributing line among Fig. 3) that is graded, and average grain diameter is 398 ± 22 μ m, and particle size distribution range is narrower; The glue pearl that rests in the fluid bed is the second batch glue pearl (seeing the red starlike distributing line among Fig. 3) that is graded, and average grain diameter is 593 ± 78 μ m, and from the result, the method can be divided into two parts according to the size of particle diameter with the glue pearl.
Claims (3)
1. the method for a classifying calcium alginate plastic beads, it is characterized in that: glue pearl to be fractionated is placed in the fluid bed, the conduct of glue pearl is phase fixedly, calcium chloride solution is as mobile phase, calcium chloride solution flows into by the lower flow entrance of peristaltic pump from fluid bed, and is inner through fluid bed, flows out from the upper flow exit at last, according to the difference of glue pearl sinking speed in fluid of different-grain diameter, make the glue pearl obtain classification by the flow velocity that changes ascending fluid;
Described fluid bed is the column type fluid bed, ratio of height to diameter is 5-20, there is the lower end to be provided with on the sidewall that a fluid intake (1), upper end be provided with a fluid issuing (2), its middle and upper part and is provided with a material outlet (3), material outlet (3) is positioned at the 2/3-5/6 of fluidized bed height, and the below, inside of fluid bed is provided with fluid distribution plate (4);
Its specific operation process is as follows,
1) glue pearl to be fractionated is placed in the fluid bed, the volume of glue pearl is fluid bed volume 1/10-2/5;
2) open peristaltic pump, the fluid intake (1) of calcium chloride solution from the fluid bed lower end slowly pumped into, open simultaneously the upper end fluid issuing (2) of fluid bed, the surface velocity of fluid is controlled at 0.01-0.1cm/s, until fluid is full of the inner chamber of whole fluid bed;
3) rotating speed of increase peristaltic pump, to improve the flow velocity of fluid, until the bed expansion height of glue pearl is when consistent with the material outlet height on the fluid bed sidewall, open material outlet (3), close simultaneously upper end fluid issuing (2), keep stablizing 5-30 minute under the constant flow rate, the glue pearl of flowing out fluid bed is first glue pearl that is graded;
4) also to continue the glue pearl of staying in the fluid bed is carried out classification, continue at again and increase rate of flow of fluid when consistent with the material outlet height on the fluid bed sidewall to the bed expansion height of glue pearl, keep stablizing 5-30 minute under the constant flow rate, the glue pearl of flowing out fluid bed is the glue pearl that second batch is graded; Carry out successively aforesaid operations, can obtain the calcium alginate plastic beads of different-grain diameter five equilibrium.
2. it is characterized in that in accordance with the method for claim 1: the concentration of described calcium chloride solution is 0.05-0.3mol/L.
3. it is characterized in that: step 3 in accordance with the method for claim 1) surface velocity of calcium chloride fluid is 0.1-0.6cm/s during classification.
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| CN 200810013524 CN101712013B (en) | 2008-10-06 | 2008-10-06 | Method for classifying calcium alginate plastic beads |
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| CN 200810013524 CN101712013B (en) | 2008-10-06 | 2008-10-06 | Method for classifying calcium alginate plastic beads |
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| CN101712013B true CN101712013B (en) | 2013-03-27 |
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| CN106140450B (en) * | 2016-07-27 | 2018-05-25 | 苏州大学 | Microsphere separation device and separation method thereof |
| CN112452528B (en) * | 2020-11-05 | 2022-04-22 | 苏州易奥秘光电科技有限公司 | Magnetic nanoparticle consistency screening method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB851067A (en) * | 1958-01-15 | 1960-10-12 | Ici Ltd | Improvements in or relating to a method of obtaining powders of uniform size |
| CN2043135U (en) * | 1989-01-04 | 1989-08-23 | 国家医药管理局上海医药工业研究院 | Beaded agarose gel screening grader |
| US5720394A (en) * | 1994-07-15 | 1998-02-24 | Yazaki Corporation | Sample supply unit for settling classification system |
| DE10020286A1 (en) * | 2000-04-26 | 2001-10-31 | Schauenburg Masch | Updraft classifier for suspensions has fine grain classifying zone after settlement zone |
-
2008
- 2008-10-06 CN CN 200810013524 patent/CN101712013B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB851067A (en) * | 1958-01-15 | 1960-10-12 | Ici Ltd | Improvements in or relating to a method of obtaining powders of uniform size |
| CN2043135U (en) * | 1989-01-04 | 1989-08-23 | 国家医药管理局上海医药工业研究院 | Beaded agarose gel screening grader |
| US5720394A (en) * | 1994-07-15 | 1998-02-24 | Yazaki Corporation | Sample supply unit for settling classification system |
| DE10020286A1 (en) * | 2000-04-26 | 2001-10-31 | Schauenburg Masch | Updraft classifier for suspensions has fine grain classifying zone after settlement zone |
Non-Patent Citations (2)
| Title |
|---|
| 叶世超，等.液—固流化床中颗粒分级和混合的研究.《高校化学工程学报》.1986,第1卷(第1期), * |
| 王庆志,等.流态化法进行粉体粒度分级实验研究.《化学工程》.2001,第29卷(第4期), * |
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Granted publication date: 20130327 Termination date: 20191006 |