CN101711891A - Artificial uterus three-dimensional gel model used for researching development and differentiation of embryonic stem cells - Google Patents
Artificial uterus three-dimensional gel model used for researching development and differentiation of embryonic stem cells Download PDFInfo
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- CN101711891A CN101711891A CN200910000700A CN200910000700A CN101711891A CN 101711891 A CN101711891 A CN 101711891A CN 200910000700 A CN200910000700 A CN 200910000700A CN 200910000700 A CN200910000700 A CN 200910000700A CN 101711891 A CN101711891 A CN 101711891A
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Abstract
The invention relates to an artificial uterus three-dimensional gel model used for researching development and differentiation of embryonic stem cells, belonging to the research field of tissue engineering and regenerative medicine, and mainly solving the problem that no ideal bionic model exists for the research of differentiation and development of embryonic stem cells in vitro. The in vitro reconstructed tissue engineered uterus is adopted as a research model of the differentiation and development of the embryonic stem cells, wherein the embryonic stem cells are inoculated on the surface or evenly combined inside the uterus lamella, the development environment of normal embryos in vivo is simulated to regulate the microenvironment of the growth and development of the embryonic stem cells, and the reconstructed uterus tissues are taken as the main body to carry out the research on differentiation and development of embryonic stem cells, thus disclosing the inherent law and mechanism of the differentiation and development of embryonic stem cells in vitro, and searching for new methods for directional differentiation of embryonic stem cells to the functional cells of important vital organs at the terminal differentiation period; and the research results have an important meaning to disclose the development of the embryos in the early period, and have important theoretical meaning and application values for the curing based on embryonic stem cells.
Description
Technical field
The invention belongs to organizational project and regenerative medicine research field, relate to the engineered uterus of external preparation, series of key techniques such as embryonic stem cell cultivation and the growth of embryonic stem cell vitro differentiation.This method can contain the engineered artificial uterus lamella or double-deck endometrium of Uterus wall three-decker in external structure, with embryonic stem cell inoculation surface or evenly be compounded in inside and make it the study model of growing as cell differentiation, and by regulating and control the microenvironment of embryonic stem cell growth, with the uterine cancer cell that reproduces is the differentiation and growth research that main body is carried out embryonic stem cell, and then the inherent law and mechanism of the growth of announcement embryonic stem cell vitro differentiation, and seek embryonic stem cell is in functioning cell directed differentiation new method from eventually last differential period to important vital organ.
Background of invention
Embryonic stem cell is the focus of scientific circles' research in recent years, and the potential of its multidirectional growth has great value and application prospect in the Tissue Engineering Study field.But the research to embryonic stem cell at present still belongs to tentatively, lacks the effective means of inducing formative tissue organ purpose cell, in vitro study system and all limited the application of embryonic stem cell in the existence of body developing environment very these present situations of big-difference.Consider above-mentioned difficulties, invent a kind of effective embryonic stem cell vitro differentiation development model and seem most important.
The uterus is the parent natural surroundings of fetal development, and period of development of embryo is played crucial effects.Embryo nidation is being supported in the parent uterus, soaks into, and growth is grown, until forming fetus.Existing studies show that, the monolayer uterine cell of In vitro culture can be used as external the building of feeder cells embryo support stem cell; The double-deck endometrium of reproducing can be used for studying embryo's ectogenesis.Consider external support effect of reproducing the uterus to fetal development, endometrial cell and reproduce the uterus external will produce the differentiation and development of embryonic stem cell what kind of the influence?
Sum up existing research, the differentiation and development model of embryonic stem cell mainly comprises the suspension hanging drop cultivation of two dimension, three-dimensional bioreactor, the three-dimensional differentiation and development model of porous polymer support and gel-like support.Intersecting at traditional two-dimentional differentiation and development cultivates, threedimensional model provides effective developmenting space for further studying the embryonic stem cell differentiation and development, but simple three supports still with body in embryo's inner cell mass differentiation and development environment in uterus very big difference is arranged, these models do not possess good bionical condition.Thus, the inventor has proposed construct and has reproduced the imagination of the bionical environment in uterus as embryonic stem cell differentiation and development model outward.
The develop rapidly of Tissue Engineering Study technology is for having established solid foundation in the external uterus of reproducing.Both at home and abroad researcher is in the external structure endometrium and carry out embryo's culture studies thereon, and this laboratory has made up three layers of comprising uterine smooth muscle and reproduced the uterus, is used to study its support effect to the embryo.The experiment basis in these existing engineered uterus reproduces model of uterus for we make up the reliable technique platform is provided.
From above research, we are with the study model of external uterus of reproducing as research embryonic stem cell differentiation and development, regulate and control the differentiation of embryonic stem cell, will be expected to solve the key technical problem in the embryonic stem cell differentiation, thereby on the embryonic stem cell differentiation and development, obtain important breakthrough.This achievement in research has important in theory meaning and using value to the treatment based on embryonic stem cell.
Summary of the invention
Be differentiation and the growth of research embryonic stem cell in external bionical environment, the invention provides and a kind ofly be used to study embryonic stem cell and grow artificial uterus three-dimensional gel model of differentiation and preparation method thereof.
Therefore, first goal of the invention of the present invention provides a kind of preparation method (referring to Fig. 1) that embryonic stem cell is grown the artificial uterus three-dimensional gel model of differentiation that is used to study, and step comprises:
With liquid collagen of I type and Matrigel glue (SIGMA, E1270) by 4: 1 mix homogeneously, then it being concentrated the culture fluid equal-volume with the DMEM-F12 that contains the uterine smooth muscle cell mixes, NaOH with 0.1M is an amount of, the mixture pH value is adjusted to 7.2～7.4, be added in the 12 orifice plate moulds that prepared, 0.4ml is injected in every hole.Be placed on 37 ℃, 5%CO
2Take out after hatching the liquid composite consolidation of 5mim in the incubator.According to identical method, the uterus stromal cell is added in the smooth muscle cell surface of having solidified, add endometrial epithelial cell with identical method again after waiting to solidify.Make the 3 D stereo artificial uterus three-dimensional gel model.
In a specific embodiments, also can respectively uterine smooth muscle cell, stromal cell and endometrial epithelial cell be mixed into the complex lamella with the extracellular matrix gel in advance, then three kinds of complex lamellas are superposeed successively, obtain having the trilaminar artificial uterus three-dimensional gel model of sandwich structure form.
In another embodiment, the wherein employed extracellular matrix gel that contains Matrigel glue is liquid in the time of 4 ℃, regulates pH=7.4, and the room temperature short time can be condensed into solid-state, and available 0.1mol/L NaOH regulates pH; Include the quantity of Mus tail collagen fiber by the glacial acetic acid unit volume in the change collagen gel preparation process, control the liquid collagen concentration of prepared I type, the liquid collagen of I type of preparation variable concentrations is used to study the growth increment situation of uterine cell under the different extracellular matrixes concentration.
In a specific embodiments, be prepared as follows the liquid collagen of I type:
I. the dislocation of cervical vertebra method is put to death the SD rat, and the Mus tail is cut off by portion from the rat root of the tail, places 75% ethanol to soak 30min;
Ii. peel off the skin of afterbody under the aseptic condition, and the Mus tail is cut into some trifles; Extract tail tendon with the ophthalmology tweezer from truncation surface, and with PBS rinsing 3 times; Tail tendon after cleaning is put into 0.1% aseptic glacial acetic acid solution, places 4 ℃ of refrigerators, or concussion to promote the tail tendon dissolving;
The centrifugal 30min of 4000rpm after iii.48 hour collects supernatant and promptly gets the liquid collagen solution of I type;
Iv. the kraft paper method is measured collagen concentration.
In another embodiment, be prepared as follows DMEM-F12 and concentrate culture fluid: one bag in DMEM-F12 dry powder, 2.2gNaHCO
3, 2.383g HEPES, 100,000 unit penicillins, 100,000 unit streptomycins are dissolved in the 500ml ultra-pure water, and regulating pH value is 7.2～7.4,0.45 μ m and 0.22 μ m filtering with microporous membrane degerming, 4 ℃ of preservations.
Also in another embodiment, be prepared as follows 12 orifice plate moulds: will be behind 2% the agar autoclaving inject 12 orifice plates, each 1ml of every hole, treat that agar solidifies after, evenly insert the aseptic capillary glass tube that 4 length are about 1cm (diameter 2mm ± 0.15mm).In super-clean bench the orifice plate lid is opened, ultra-vioket radiation mould 30min puts it into 37 ℃, 5%CO afterwards
2Stand-by in the incubator.
In another embodiment, the outer three dimensional gel model structure of wherein said body of uterus also can add the interpolation gonadal hormone, with the physiological environment of growing in the body of regulating uterus propagation secretion cycle and simulation embryo, wherein gonadal hormone comprises estrogen, progestogen and/or androgen, and addition manner can be to be undertaken successively or compound interpolation by the normal physiological periodic law.
Second purpose of the present invention provides the artificial uterus three-dimensional gel model by method for preparing.
In a specific embodiments, described artificial uterus three-dimensional gel model wherein can adopt static tensile mode, by glass column mould fixed model lamella, and the mechanical environment of growing in the body with the simulation embryo.
The 3rd purpose of the present invention is by described artificial uterus three-dimensional gel model, and a kind of method that intrauterine embryonic liver cell differentiation is grown of studying is provided, and comprising:
(1) embryonic stem cell is seeded in the artificial uterus three-dimensional gel surface that has prepared with unicellular form, divide a word with a hyphen at the end of a line to the three-dimensional lamella in uterus and soak into or implant by observing the suspend embryoid body sample that forms of embryonic stem cell, and in lamella the phenomenon of differentiation and development, explore embryonic stem cell and early embryo development incident; Or
(2) embryonic stem cell is seeded in inside, artificial uterus with unicellular form, promptly directly with single celled form with after endometrial cell mixes, make in the complex lamella with the extracellular matrix gel, and the method for claim 1 makes artificial uterus three-dimensional gel model, to observe the differentiation and development phenomenon of embryonic stem cell in the lamella of uterus.
In a specific embodiments, wherein also can in artificial uterus three-dimensional gel model, add different inducible factors, observe its influence to the embryonic stem cell differentiation, described inducible factor comprises: Vc, RA, activin-A, TGF and IGF.
The 4th purpose of the present invention is by described artificial uterus three-dimensional gel model, and a kind of method of studying the differentiation and development of embryonic stem cell in external uterine inner membrance microenvironment is provided, and comprising:
(1) embryonic stem cell directly is compounded in the simple extracellular matrix gel, observes the spontaneous differentiation and development situation of embryonic stem cell;
(2) embryonic stem cell is compounded in the inner membrance lamella of artificial uterus three-dimensional gel, observing the endometrium microenvironment is the influence of embryonic stem cell differentiation and development.
In a specific embodiments, wherein also can in artificial uterus three-dimensional gel model, add different inducible factors, observe its influence to the embryonic stem cell differentiation, described inducible factor comprises: Vc, RA, activin-A, TGF and IGF.
The 5th purpose of the present invention provides a kind of artificial uterus three-dimensional gel model and is used to study the method that embryonic stem cell is induced differentiation and grown.Wherein said purposes is the microenvironment by regulation and control embryonic stem cell growth promoter, carrying out embryonic stem cell induces differentiation and grows research, and then the inherent law that announcement embryonic stem cell vitro differentiation is grown is with machine-processed, and seek embryonic stem cell and be in the functioning cell directed differentiation of whole end differential period (as cardiac muscle to important vital organ, nerve, blood vessel) new method.
Should be pointed out that the present invention aims to provide a kind of preparation of extracellular matrix gel model, be not intended to various cells related in this product.For wherein said cell, as materials such as, endometrial epithelial cell, uterine smooth muscle cell and Interstitial cells, endometrial epithelial cell, uterine smooth muscle cell and Interstitial cell can use prior art to be prepared (Establishment of a human endometrial cell culture system and characterization of its polarizedhormone responsive epithelial cells
Cell Tissue Res.1997 Jan; 287 (1): 171-85.); The cell line of perhaps using common lab to carry out preservation after the separation and Culture according to conventional methods, the perhaps cell line of commodity in useization.For example, the present invention uses and to have built the embryonic stem cell that is, the TC-1 cell that is provided by BIO ENGINEERING INST MILITARY.
Description of drawings
Fig. 1: external structure contains the engineered artificial uterus lamella flow chart of Uterus wall three-decker
Fig. 2: external structure contains the engineered artificial uterus lamella of Uterus wall three-decker
A: the gross examination of skeletal muscle of the engineered uterus lamella of external structure; B: engineered artificial uterus lamella * 5 that contain the Uterus wall three-decker;
Fig. 3: embryonic stem cell is seeded in the uterus sheet surfaces
A: embryoid body * 10 that embryonic stem cell forms; B: the embryoid body that embryonic stem cell forms to endometrium divide a word with a hyphen at the end of a line * 40
Fig. 4: the embryonic stem cell of DAPI labelling evenly is compounded in inner embryoid body * 10 that form of uterus lamella
Fig. 5: embryonic stem cell forms terminally differentiated cells in the lamella of uterus
A:VIII factor dyeing * 20; : B:cTnT dyeing * 20;
The specific embodiment
Only further describe the present invention now with mode with reference to following non-restrictive example.But should be appreciated that the following examples only as illustration, should be by any way when doing the restriction overall to the invention described above.Unless other explanation is arranged, the traditional molecular biology in embodiments of the invention use this area, cytobiology, tissue engineering or the like.These technology are that the technical staff knows, and detailed explanation is arranged in the literature.Referring to, for example, Fabrication of viable tissue-engineeredconstructs with3D cell-assembly technique " (2005); Extracellular matrix-enriched polymericscaffolds as a substrate for hepatocyte cultures:in vitro and in vivo studies " (2005).Embodiment 1. external structures contain the engineered artificial uterus lamella or double-deck endometrium of the three-decker of Uterus wall:
The engineered artificial uterus of the three-decker of external structure lamella flow process as shown in Figure 1.Specific as follows: that liquid collagen of I type and Matrigel glue are pressed 4: 1 mix homogeneously, then it being concentrated the culture fluid equal-volume with the DMEM-F12 that contains the uterine smooth muscle cell mixes, NaOH with 0.1M is an amount of, the mixture pH value is adjusted to 7.2～7.4, be added in the 12 orifice plate moulds that prepared, 0.4ml is injected in every hole.Be placed on 37 ℃, 5%CO
2Take out after hatching the liquid composite consolidation of 5mim in the incubator.According to identical method, the uterus stromal cell is added in the smooth muscle cell surface of having solidified, add endometrial epithelial cell with identical method again after waiting to solidify.Make 3 D stereo artificial uterus three-dimensional gel model (as shown in Figure 2).Add estrogen and progestogen, regulate the uterus propagation secretion cycle.
Embodiment 2. enters external bionic model with embryonic stem cell with surface seeding or inner even compound dual mode:
1) embryonic stem cell is seeded in the uterus sheet surfaces: embryonic stem cell is seeded in surface (as shown in Figure 3) in the uterus sheet that has prepared with single celled form, observe the suspend embryoid body sample that forms of embryonic stem cell and move shape to the uterus lamella and implant, and in lamella the phenomenon of differentiation and development;
2) embryonic stem cell is mixed with endometrial cell with single celled form, add in the collagen gel, regulate acid-base value to neutral with 0.1mol/LNaOH, add 10% Matrigel again in 4: 1 ratio, structure is observed the differentiation and development phenomenon (as shown in Figure 4) of embryonic stem cell in the lamella of uterus with the compound uterus lamella wherein of embryonic stem cell.
Embodiment 3. embryonic stem cells are induced differentiation and are grown research:
In the embryonic stem cell uterus differentiation and development model that has made up, add different derivants, observe the ability of embryonic stem cell to different adult cell directional differentiation and developments.Figure 5 shows that the detection case embryonic stem cell forms terminally differentiated cells in the lamella of uterus after.
Claims (10)
1. study the preparation method that embryonic stem cell is grown the artificial uterus three-dimensional gel model of differentiation for one kind, step comprises:
Liquid collagen of I type and Matrigel glue are pressed 4: 1 mix homogeneously, then it being concentrated the culture fluid equal-volume with the DMEM-F12 that contains the uterine smooth muscle cell mixes, NaOH with 0.1M is an amount of, the mixture pH value is adjusted to 7.2～7.4, be added in the 12 orifice plate moulds that prepared, 0.4ml is injected in every hole; With
Be placed on 37 ℃, 5%CO
2Take out after hatching the liquid composite consolidation of 5mim in the incubator; With
According to identical method, the uterus stromal cell is added in the smooth muscle cell surface of having solidified, add endometrial epithelial cell with identical method again after waiting to solidify, make the 3 D stereo artificial uterus three-dimensional gel model.
2. the described preparation method of claim 1, wherein respectively uterine smooth muscle cell, stromal cell endometrial epithelial cell are mixed into the complex lamella with the extracellular matrix gel in advance, then three kinds of complex lamellas are superposeed successively, obtain having the trilaminar artificial uterus three-dimensional gel model of sandwich structure form.
3. claim 1 or 2 described preparation methoies, the wherein employed extracellular matrix gel that contains Matrigel glue is liquid in the time of 4 ℃, regulates pH=7.4, the room temperature short time can be condensed into solid-state, and available 0.1mol/L NaOH regulates pH; Include the quantity of Mus tail collagen fiber by the glacial acetic acid unit volume in the change collagen gel preparation process, control the liquid collagen concentration of prepared I type, the liquid collagen of I type of preparation variable concentrations is used to study the growth increment situation of uterine cell under the different extracellular matrixes concentration.
4. claim 1 or 2 described preparation methoies, wherein in prepared 3 D stereo extracellular matrix gel model, add different gonadal hormone, the jellium model that is used for ESC directional induction differentiation research with preparation, described gonadal hormone can comprise estrogen, progestogen and/or androgen, and addition manner can be to be undertaken successively or compound interpolation by the normal physiological periodic law.
5. one kind is passed through the artificial uterus three-dimensional gel model that each described method of claim 1 to 4 prepares.
6. one kind is passed through the described artificial uterus three-dimensional gel model of claim 5, studies the method that intrauterine embryonic liver cell differentiation is grown.
7. the described method of claim 7, comprising:
1) embryonic stem cell is seeded in the artificial uterus three-dimensional gel surface that has prepared with unicellular form, divide a word with a hyphen at the end of a line to the three-dimensional lamella in uterus and soak into or implant by observing the suspend embryoid body sample that forms of embryonic stem cell, and in lamella the phenomenon of differentiation and development, explore embryonic stem cell and early embryo development incident; Or
2) embryonic stem cell is seeded in inside, artificial uterus with unicellular form, promptly directly with single celled form with after endometrial cell mixes, make in the complex lamella with the extracellular matrix gel, and the method for claim 1 makes artificial uterus three-dimensional gel model, to observe the differentiation and development phenomenon of embryonic stem cell in the lamella of uterus.
8. one kind is passed through the described artificial uterus three-dimensional gel model of claim 5, studies the method for the differentiation and development of embryonic stem cell in external uterine inner membrance microenvironment, comprising:
(1) embryonic stem cell directly is compounded in the simple extracellular matrix gel, observes the spontaneous differentiation and development situation of embryonic stem cell;
(2) embryonic stem cell is compounded in the inner membrance lamella of artificial uterus three-dimensional gel, observing the endometrium microenvironment is the influence of embryonic stem cell differentiation and development.
9. the described method of claim 8 wherein can be added different inducible factors in artificial uterus three-dimensional gel model, observes its influence to the embryonic stem cell differentiation, and described inducible factor comprises: Vc, RA, activin-A, TGF and IGF.
10. one kind is passed through the described artificial uterus three-dimensional gel model of claim 5, be used to study embryonic stem cell and induce differentiation and the purposes of growing, wherein said purposes is the microenvironment by regulation and control embryonic stem cell growth promoter, carrying out embryonic stem cell induces differentiation and grows research, and then the inherent law that announcement embryonic stem cell vitro differentiation is grown is with machine-processed, and seek embryonic stem cell and be in the functioning cell directed differentiation of whole end differential period (as cardiac muscle to important vital organ, nerve, blood vessel) new method.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031877A (en) * | 2014-05-14 | 2014-09-10 | 浙江大学 | Preparation method for model simulating in-vitro implantation process of early embryo into endometrium |
| CN106434536A (en) * | 2016-08-10 | 2017-02-22 | 何祖平 | Three-dimensional inducing method for inducing spermatogonia stem cell to differentiate into functional sperm cell in vitro |
| CN108261566A (en) * | 2018-02-26 | 2018-07-10 | 陕西九州生物医药科技集团有限公司 | A kind of preparation method of menses stem cell diaphragm |
| CN110885781A (en) * | 2018-09-07 | 2020-03-17 | 中国科学院大连化学物理研究所 | Human early placenta development model establishing method based on organ chip |
-
2009
- 2009-01-15 CN CN200910000700A patent/CN101711891A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031877A (en) * | 2014-05-14 | 2014-09-10 | 浙江大学 | Preparation method for model simulating in-vitro implantation process of early embryo into endometrium |
| CN106434536A (en) * | 2016-08-10 | 2017-02-22 | 何祖平 | Three-dimensional inducing method for inducing spermatogonia stem cell to differentiate into functional sperm cell in vitro |
| CN106434536B (en) * | 2016-08-10 | 2019-07-30 | 何祖平 | People's Spermatogonial Stem Cells are divided into the three-dimensional abductive approach of function spermatoblast |
| CN108261566A (en) * | 2018-02-26 | 2018-07-10 | 陕西九州生物医药科技集团有限公司 | A kind of preparation method of menses stem cell diaphragm |
| CN110885781A (en) * | 2018-09-07 | 2020-03-17 | 中国科学院大连化学物理研究所 | Human early placenta development model establishing method based on organ chip |
| CN110885781B (en) * | 2018-09-07 | 2023-02-17 | 中国科学院大连化学物理研究所 | Human early placenta development model establishing method based on organ chip |
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Application publication date: 20100526 |