CN101708342A - Temperature sensitive wound face dressing film and preparation method thereof - Google Patents
Temperature sensitive wound face dressing film and preparation method thereof Download PDFInfo
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- CN101708342A CN101708342A CN200910200542A CN200910200542A CN101708342A CN 101708342 A CN101708342 A CN 101708342A CN 200910200542 A CN200910200542 A CN 200910200542A CN 200910200542 A CN200910200542 A CN 200910200542A CN 101708342 A CN101708342 A CN 101708342A
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Abstract
The invention relates to a temperature sensitive wound face dressing film and a preparation method thereof, which belongs to the technical field of biologic medical high molecular materials. In the method, vinyl alcohol (PVA) and a glycan-grafted N-isopropyl acylamide polymer (CS-g-PNIPAAm) are mixed together, then the procedures of injection molding, dried filming, sterilization and the like are carried out, and finally, the temperature sensitive wound face dressing film with the function of slow release is prepared. The gel dressing film combines the original advantages of polyvinyl alcohol and chitosan, has better transparence, water absorptivity and humidity preservation property, is not conglutinated with regenerated tissues and can effectively prevent the infection of a wound face. When being used, the dressing film carrying a medicine generates reversible swelling and contraction along with the temperature change of the wound face by medicine carrying, and the medicine generates circular opening and closing release along with the dressing film, thereby achieving the intellectualized control of the release of the medicine, promoting the healing of the wound face and enhancing the curative effect.
Description
Technical field
The present invention relates to a kind of temperature sensitive wound face dressing film and preparation method thereof, belong to the biological medical polymer material technical field.
Background technology
Burn, scald of common occurrence in daily life, because its degree of depth of wound surface that forms, the difference in orientation if adopt traditional hospital gauze or cotton yarn class dressing method to treat, not only cause traumatic infection easily, cause various complication, thereby cause the systemic infection symptom; And the restore cycle is long, causes secondary damage easily; There is problem such as obvious cicatrix perplexing countless patients after wound surface recovers, this shows that the effect of repairing phase of traditional treatment method is often not fully up to expectations.That if wound-surface cover (or deposited film) has is antibiotic, stop blooding, preserve moisture, hydrophilic, have the function that can discharge medicine not with cambium adhesion performance the time according to the situation of wound surface, will help promoting wound healing, reduce the patient suffering, improve curative effect.
Chitosan and polyvinyl alcohol are made membranaceous by simple mechanical blending or the gelationus deposited film of water-setting, and its function is these two kinds of synergistic results of material original function.Studies show that the PVA/CS hydrogel applies film and compares with traditional deposited film, it has possessed many traditional unexistent premium properties of deposited film, as: the juice that can absorb wound forms protective layer; Aseptic nontoxic, airtight but ventilative simultaneously, water vapour permeability is good, can play the effect of protection wound surface effectively, prevents wound inflammation; Treatment wound surface effect is obvious, and the number of times that need change dressings reduces, and has avoided secondary damage as much as possible, and wound healing time obviously shortens, and slows down patient's misery; Apply film and wound surface sticking apply closely but not with the wound surface adhesion, more during change dressings to new epithelize and the destruction of granulation tissue not damaged, and the wound does not have the delay of dressing residue; Have no side effect, do not have advantages such as bringing out scar hyperplasia.
PVA/CS applies film and combines chitosan and polyvinyl alcohol advantage separately, has remedied the shortcoming of one-component, has improved the performance of deposited film self, now has been subjected to extensive concern and furthers investigate.The present invention adopts the get everything ready hydrogel wound face dressing film of temperature sensitivity of polyvinyl alcohol and chitosan graft poly-N-isopropyl acrylamide (CS-g-PNIPAAm) physical crosslinking legal system, on the basis of having acted on the deposited film advantage of PVA/CS, introduce temperature sensitivity, it is had varies with temperature and reversible " swelling "-" contraction " function takes place, realized the medicine circulation " open "-" pass " discharge.This wound face dressing film with temperature sensitivity does not have relevant report so far as yet.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of temperature sensitive wound face dressing film.
The present invention is a kind of preparation method of temperature sensitive wound face dressing film, it is characterized in that having following process and step:
A. the preparation of polyvinyl alcohol (PVA) aqueous solution: the PVA powder is joined in the deionized water, and both mass ratioes are PVA: H
2O=10: 90; Carry out heating in water bath under 80～90 ℃, heat while stirring, dissolve fully until PVA, form the solution of clear, at room temperature cooling is stand-by;
B. the preparation of chitosan graft poly-N-isopropyl acrylamide (CS-g-PNIPAAm): at normal temperatures,, be made into the homogeneous transparent solution of 2% (w/v) chitosan (CS) with 1% (v/v) HAc solution dissolving chitosan (CS) powder; Get a certain amount of N-N-isopropylacrylamide, join in a certain amount of 2% chitosan (CS) solution; Both mass ratioes are chitosan (CS): N-N-isopropylacrylamide (NIPAAm)=1: 1; Stir, treat that powder dissolves fully after, pour in the there-necked flask; In above-mentioned solution, charged into nitrogen about 40 minutes, and then initiator potassium persulfate (KPS) solution was joined in the there-necked flask; The addition of KPS is 0.3% (w/w) of CS quality; Stir on logical nitrogen limit, limit, stir 10 minutes fast after, be put in 70 ℃ of stirred in water bath reactions 2 hours again; Reaction finishes postcooling to room temperature, reactant liquor is splashed into to a large amount of acetone by the method that magnetic agitation limit, limit drips, treat abundant post precipitation, with precipitate with a large amount of washing with acetones 2 times, the excessive ethyl acetate of reuse precipitates and washs 1 time, removing unreacted NIPAAm, with the CS-g-PNIPAAm powder of finally separating out at 40 ℃ of following constant temperature dryings; Stand-by;
C. be 1% HAc solution dissolving with the CS-g-PNIPAAm powder volumetric concentration of above-mentioned gained, both mass volume ratios are 4% (w/v); After treating to dissolve fully, the PVA solution mixed together that is prepared with above-mentioned steps a; Its proportioning is: PVA: CS=1: 4～4: 1, and keeping total solid content is 5% (w/v); To mix gained solution and at the uniform velocity stir 24 hours, at room temperature leave standstill a period of time after, the evacuation froth breaking;
D. above-mentioned mixed solution is poured in the specific system film die then, be put in and dry after 2 days in 40 ℃ of baking ovens, make certain thickness film, it is in the NaOH solution of 0.4% (w/v) that described film is immersed a large amount of concentration, immersion after 2 hours with a large amount of deionized waters surface of flushing membrane repeatedly; Dried 12 hours in 40 ℃ of baking ovens then, the electron beam width of cloth that re-uses 25KGy dosage finally makes temperature sensitive wound face dressing film according to sterilization; Its sealing sterilization is kept, stand-by.
The purposes and the using method of the prepared wound face dressing film of preparation method of a kind of temperature sensitive wound face dressing film of the present invention, prepared temperature sensitive wound face dressing film can load various antibiotic, pain relievings, hemostasis class medicine; Make it intelligent sustained release take place under with the wound surface variations in temperature at deposited film; The method of medicine carrying has leading portion medicine carrying method and back segment medicine carrying method: leading portion medicine carrying method is to apply preload in the membrane process in preparation; Back segment medicine carrying method is to carry out swelling again to suck the medicine carrying processing before deposited film finished product uses.
The present invention adopts the get everything ready hydrogel wound face dressing film of temperature sensitivity of polyvinyl alcohol and chitosan graft poly-N-isopropyl acrylamide physical crosslinking legal system, acted on polyvinyl alcohol/chitosan and applied on the advantage basis of film and introduce temperature sensitivity, it has been had vary with temperature and reversible " swelling ", " contraction " function take place.Realize that the medicine circulation " is opened ", " pass " discharges.
It is reported, human body under normal circumstances, shell temperature is roughly 31-33 ℃; And be subjected to courses of infection when the surface of being wound, and cause systemic infection or focal infection, thereby cause part or whole body heating, cause shell temperature to rise.According to this characteristic, the present invention adopts grafting on the chitosan chain to introduce the method for poly-N-isopropyl acrylamide and polyvinyl alcohol blending with temperature sensitivity, makes the deposited film of the hydrogel with temperature sensitive property.PNIPAAm is a kind of typical temperature sensitive hydrogel, and its phase critical transition temperature (LCST) is about 33 ℃.Gel has good hydrophilicity when temperature is lower than LCST, and slow releasing function is obvious, and when temperature was higher than LCST, the hydrophobic contraction of gel caused the gel internal moisture to be overflowed, and drug release rate is accelerated.(referring to Fig. 1)
The present invention adopts PVA and CS-g-PNIPAAm blend, injection molding, and drying and forming-film, technologies such as sterilization are made the hydrogel wound face dressing film that preparation has temperature sensitive property.Should apply the advantage that film combines PVA and CS, and load all kinds of antibiotic, pain relievings, hemostasis class medicine as required, reversible " swelling "-" contraction " takes place with the wound surface variations in temperature, forming medicine " opening "-" pass " capable of circulation discharges, thereby reach intelligent sustained release medicine, promote wound healing, improve the purpose of curative effect, remedied the shortcoming of traditional therapy well.
The advantage and the characteristics of the inventive method are as follows:
1, apply film among the present invention and have good water absorption, swellability is better when (normal shell temperature) below 33 ℃, and water absorption rate is 8-10 doubly (w/w); When temperature is higher than 33 ℃ (body surface that causes because of infection, heating etc. or wound surface temperature rise), apply the swellability variation of film and shrink, water absorption rate is reduced to 3-5 doubly.And deposited film varies with temperature and variation that " swelling " and " contraction " take place is repeatedly reversible.
2, apply film among the present invention and compare with traditional hospital gauze dressing, can prevent bacterial invasion, the wound that adequately protects is avoided polluting; The water absorption rate height, expanding behind the absorption secretions forms the gel protective layer; Directly be pasted on wound, adhesion strength is strong, and is easy to use; With the cambium adhesion, be difficult for producing the secondary wound.
Description of drawings
Fig. 1 presents the drug release sketch map of " on-off " formula under different temperatures for temperature sensitive property wound face dressing film among the present invention.
The specific embodiment
Now specific embodiments of the invention are described in detail in after.
Embodiment 1: below be among the present invention the preparation novel temperature sensitive property wound face dressing film example, its technical process and step are as follows:
1, get the 20gPVA powder and be put in the beaker, add the 180g deionized water again, heat while stirring in 90 ℃ of water-baths, dissolve fully until PVA, make 10% (w/w) PVA solution, cooling is stand-by under the room temperature.
2, measure the 5ml glacial acetic acid,, make 500ml 1%HAc solution with standardize solution in the 500ml volumetric flask.Take by weighing the 3gCS powder, with the dissolving of 150ml 1%HAc solution, treat that solid dissolves fully after, add the 3gNIPAAm monomer, be stirred to dissolving fully.Mixed solution is poured in the there-necked flask, at normal temperatures preliminary filling N
240 minutes.Take by weighing 0.0100gKPS, use the 1ml deionized water dissolving, dropwise add in the there-necked flask, after waiting to drip end, room temperature quickens down to stir 10 minutes.Reaction is transferred in 70 ℃ of water-baths, reacted 2 hours, overall process is all at N
2Carry out under the protection.
3, after the reactant liquor for the treatment of step 2 gained is cooled to room temperature, under agitation splash into small amount of acetone, stirring presents magnetic agitation limit, emulsion form back and dropwise adds in a large amount of acetone, drips to finish back restir half an hour, sucking filtration.Behind the powder that goes out with excessive washing with acetone sucking filtration 2 times, excessive ethyl acetate precipitation, the washing of reuse 1 time.The gained powder is 40 ℃ of drying in oven, and is to constant mass, stand-by.
4, measure the graft powder of 150ml 1%HAc solution dissolving step 3 gained, treat to dissolve the 10%PVA solution that the back adds 30g fully, at the uniform velocity stirred 24 hours.Solution is transferred in the container of 10cm * 10cm, leaves standstill froth breaking under the room temperature, treat that solution surface bubble froth breaking finishes after, pour the system film die into, be transferred in 40 ℃ of baking ovens, dried 2 days.The film of oven dry is soaked into fully in excessive 0.4%NaOH, take out after 2 hours, present neutrality, prepared wet film is positioned over 40 ℃ of drying in oven, uses 25KGy dosage electron beam irradiation sterilization with a large amount of deionized water rinsings to surface; Finally make temperature sensitive wound face dressing film.
Claims (2)
1. the preparation method of a temperature sensitive wound face dressing film is characterized in that having following process and step:
A. the preparation of polyvinyl alcohol (PVA) aqueous solution: the PVA powder is joined in the deionized water, and both mass ratioes are PVA: H
2O=10: 90; Carry out heating in water bath under 80～90 ℃, heat while stirring, dissolve fully until PVA, form the solution of clear, at room temperature cooling is stand-by;
B. the preparation of chitosan graft poly-N-isopropyl acrylamide (CS-g-PNIPAAm): at normal temperatures,, be made into the homogeneous transparent solution of 2% (w/v) chitosan (CS) with 1% (v/v) HAc solution dissolving chitosan (CS) powder; Get a certain amount of N-N-isopropylacrylamide, join in a certain amount of 2% chitosan (CS) solution; Both mass ratioes are chitosan (CS): N-N-isopropylacrylamide (NIPAAm)=1: 1; Stir, treat that powder dissolves fully after, pour in the there-necked flask; In above-mentioned solution, charged into nitrogen about 40 minutes, and then initiator potassium persulfate (KPS) solution was joined in the there-necked flask; The addition of KPS is 0.3% (w/w) of CS quality; Stir on logical nitrogen limit, limit, stir 10 minutes fast after, be put in 70 ℃ of stirred in water bath reactions 2 hours again; Be cooled to room temperature then, reactant liquor is splashed in a large amount of acetone by the method that magnetic agitation limit, limit drips, treat abundant post precipitation, with precipitate with a large amount of washing with acetones 2 times, the excessive ethyl acetate of reuse precipitates and washs 1 time, removing unreacted NIPAAm, with the CS-g-PNIPAAm powder of finally separating out at 40 ℃ of following constant temperature dryings; Stand-by;
C. be 1% HAc solution dissolving with the CS-g-PNIPAAm powder volumetric concentration of above-mentioned gained, both mass volume ratios are 4% (w/v); After treating to dissolve fully, the PVA solution mixed together that is prepared with above-mentioned steps a; Its proportioning is: PVA: CS=1: 4～4: 1, and keeping total solid content is 5% (w/v); To mix gained solution and at the uniform velocity stir 24 hours, at room temperature leave standstill a period of time after, the evacuation froth breaking;
D. above-mentioned mixed solution is poured in the specific system film die then, be put in and dry after 2 days in 40 ℃ of baking ovens, make certain thickness film, it is in the NaOH solution of 0.4% (w/v) that described film is immersed a large amount of concentration, immersion after 2 hours with a large amount of deionized waters surface of flushing membrane repeatedly; Dried 12 hours in 40 ℃ of baking ovens then, the electron beam width of cloth that re-uses 25KGy dosage finally makes temperature sensitive wound face dressing film according to sterilization; Its sealing sterilization is kept, stand-by.
2. the purposes and the using method of the prepared wound face dressing film of preparation method of a temperature sensitive wound face dressing film, prepared temperature sensitive wound face dressing film can load various antibiotic, pain relievings, hemostasis class medicine; Make it intelligent sustained release take place under with the wound surface variations in temperature at deposited film; The method of medicine carrying has leading portion medicine carrying method and back segment medicine carrying method: leading portion medicine carrying method is to apply preload in the membrane process in preparation; Back segment medicine carrying method is to carry out swelling again to suck the medicine carrying processing before deposited film finished product uses.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103212108A (en) * | 2013-05-04 | 2013-07-24 | 四川师范大学 | Collagen wound healing film and preparation method thereof |
| CN104354491A (en) * | 2014-09-30 | 2015-02-18 | 浙江理工大学 | Child painting brush |
| CN108373553A (en) * | 2017-12-29 | 2018-08-07 | 佛山市锦彤企业管理有限公司 | A kind of response type antimicrobial macromolecule hydrogel |
| CN114917398A (en) * | 2022-05-06 | 2022-08-19 | 深圳市世格赛思医疗科技有限公司 | Tissue adhesive material capable of shrinking wound and preparation method and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341899C (en) * | 2005-11-01 | 2007-10-10 | 中国药科大学 | Derivatives of new chitosan, preparation method, and application in use for making ophthalmic preparation |
| CN1974608A (en) * | 2006-11-24 | 2007-06-06 | 清华大学 | Electronic beam radiopolymerization process of directly synthesizing temperature sensitive hydrogel |
| CN1974609A (en) * | 2006-11-24 | 2007-06-06 | 清华大学 | Electronic beam radiopolymerization process of directly synthesizing pH sensitive polymer hydrogel |
| KR100868724B1 (en) * | 2007-05-30 | 2008-11-13 | 한국생산기술연구원 | Method for preparing self-aggregating nanocarrier particles having temperature depending property |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103212108A (en) * | 2013-05-04 | 2013-07-24 | 四川师范大学 | Collagen wound healing film and preparation method thereof |
| CN103212108B (en) * | 2013-05-04 | 2014-06-11 | 四川师范大学 | Collagen wound healing film and preparation method thereof |
| CN104354491A (en) * | 2014-09-30 | 2015-02-18 | 浙江理工大学 | Child painting brush |
| CN104354491B (en) * | 2014-09-30 | 2016-08-24 | 浙江理工大学 | Child's paintbrush |
| CN108373553A (en) * | 2017-12-29 | 2018-08-07 | 佛山市锦彤企业管理有限公司 | A kind of response type antimicrobial macromolecule hydrogel |
| CN114917398A (en) * | 2022-05-06 | 2022-08-19 | 深圳市世格赛思医疗科技有限公司 | Tissue adhesive material capable of shrinking wound and preparation method and application thereof |
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| CN101708342B (en) | 2012-10-31 |
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