CN101696445A - Method for screening microbial flora used for treating coal chemical industrial waste water - Google Patents
Method for screening microbial flora used for treating coal chemical industrial waste water Download PDFInfo
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- CN101696445A CN101696445A CN200910073092A CN200910073092A CN101696445A CN 101696445 A CN101696445 A CN 101696445A CN 200910073092 A CN200910073092 A CN 200910073092A CN 200910073092 A CN200910073092 A CN 200910073092A CN 101696445 A CN101696445 A CN 101696445A
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Abstract
The invention discloses a method for screening a microbial flora used for treating coal chemical industrial waste water, which relates to a method for screening a microbial flora. The invention solves the problem that the traditional screening method has complex process and that the obtained microbial flora has a poor treatment effect on coal chemical industrial waste water. The method comprises: 1, aerating a sludge and waste water mixture; 2, adding a medicament to the mixture of the sludge and the waste water, mixing the mixture, allowing the mixture to stand and obtaining settled sludge; and 3, acclimating the settled sludge, and thus realizing the screening of the microbial flora for treating the coal chemical industrial waste water. The method has a simple screening process. The screened microbial flora has an excellent treatment effect on the coal chemical industrial waste water.
Description
Technical field
The present invention relates to the screening method of microorganism species.
Background technology
The quantity discharged of the average every day of coal chemical industry enterprises waste water has reached 9000m
3More than, this waste water is based on the high density gas washing wastewater, and water quality is very complicated, contains a large amount of phenols, alkanes, arene, heterocyclic, ammonia nitrogen, cyanogen, wet goods hazardous and noxious substances.At present, the method of handling coal chemical industrial waste water adopts activated sludge process more, but the biological species of activated sludge process is various, to the poor processing effect of coal chemical industrial waste water, existing method by bacterial screening obtains the dominant microflora that Coal Chemical Industry is handled, and improves the treatment effect of activated sludge process.Mostly existing screening method is the bacterial classification by single certain material in the screening degraded coal chemical industrial waste water, such as efficient phenol degradation bacteria, cyanide degradation bacterium, ammonia nitrogen degradation bacterium etc., though such method specific aim is very strong, but because the unicity of degradation product in the screening, cause a lot of bacterial classifications in actual applications intermediate product in other chemical substance resistibility differences or the degradation process in the coal chemical industrial waste water excessively to be accumulated and suppress to continue degraded, can't bring into play due effect in actual engineering, the coal chemical industrial waste water treatment effect is relatively poor; Another kind of screening method is that the specific bacterial classification of screening makes up flora, artificially controlled to reach flora, owing to the ratio that adds of a bacterial strain in the actually operating is difficult to determine, cause the original idea of this method to implement at last, some mud does not even increase in the treatment project of coal chemical industrial waste water, adopt this method process complexity, and screening obtains the treatment effect extreme difference of flora to coal chemical industrial waste water.
Summary of the invention
The objective of the invention is in order to solve existing screening method process complexity, the microorganism species that obtains of screening problem, and the screening method of the microorganism species that is used to handle coal chemical industrial waste water is provided the coal chemical industrial waste water poor processing effect.
The screening method that the present invention is used to handle the microorganism species of coal chemical industrial waste water carries out according to following steps:
One, the mud waste water mixture in the coal chemical industrial waste water treatment system is placed aeration 13～16h under 34～36 ℃ of conditions, leave standstill 8～11h then; Two, the MgSO that adds 0.4～0.6g in the mud waste water mixture after every liter of step 1 is handled
47H
2The FeSO of O, 0.05～0.15g
47H
2The ZnSO of O, 0.005～0.015g
47H
2The MnSO of O, 0.005～0.015g
4H
2The CuSO of O, 0.005～0.015g
45H
2The NH of O, 0.2～0.3g
4The CaCl of Cl, 0.05～0.15g
2Stir with the sucrose of 2～4g, aeration 14～16h under 34～36 ℃ of conditions leaves standstill and discards supernatant liquid behind 8～10h and get sedimentation mud; Three, sedimentation mud is placed the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 1 generation domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 4 volume ratio coal chemical industrial waste water and water; Four, the sedimentation mud with the domestication of 1 generation places the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes to tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 2 generations domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 2 volume ratio coal chemical industrial waste water and water; Five, the sedimentation mud of 2 generations domestications is accounted in the coal chemical industrial waste water of 3～5 times of sedimentation mud cumulative volumes and tame, tame to the biomass of sedimentation mud and reach 3800～4100mg/mL, promptly realized being used to handling the screening of the microorganism species of coal chemical industrial waste water; Wherein the domestication condition in step 3, step 4 and the step 5 all is to carry out under 34～36 ℃ of conditions, first anoxia stirring 3～4h, and aeration is cultivated 14～16h then.
Method of the present invention is got the mud waste water mixture in the coal chemical industrial waste water treatment system, through enrichment culture, and then tames, and obtains handling the microorganism species of coal chemical industrial waste water.Screening method of the present invention is drawn materials conveniently, screening process is simple, the microorganism species that utilizes the present invention to screen to obtain is handled coal chemical industrial waste water, result shows, the degradation rate of COD is 80%～90% in the coal chemical industrial waste water, the degradation rate of phenol is 90%～99.9%, and the degradation rate of prussiate is 90%～99.9%, the degradation rate of ammonia nitrogen is 35%～50%, and the microorganism species that the present invention's screening obtains is good to the treatment effect of coal chemical industrial waste water.
Embodiment
Embodiment one: the screening method that present embodiment is used for handling the microorganism species of coal chemical industrial waste water carries out according to following steps: one, the mud waste water mixture with the coal chemical industrial waste water treatment system places aeration 13～16h under 34～36 ℃ of conditions, leaves standstill 8～11h then; Two, the MgSO that adds 0.4～0.6g in the mud waste water mixture after every liter of step 1 is handled
47H
2The FeSO of O, 0.05～0.15g
47H
2The ZnSO of O, 0.005～0.015g
47H
2The MnSO of O, 0.005～0.015g
4H
2The CuSO of O, 0.005～0.015g
45H
2The NH of O, 0.2～0.3g
4The CaCl of Cl, 0.05～0.15g
2Stir with the sucrose of 2～4g, aeration 14～16h under 34～36 ℃ of conditions leaves standstill and discards supernatant liquid behind 8～10h and get sedimentation mud; Three, sedimentation mud is placed the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 1 generation domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 4 volume ratio coal chemical industrial waste water and water; Four, the sedimentation mud with the domestication of 1 generation places the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes to tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 2 generations domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 2 volume ratio coal chemical industrial waste water and water; Five, the sedimentation mud of 2 generations domestications is accounted in the coal chemical industrial waste water of 3～5 times of sedimentation mud cumulative volumes and tame, tame to the biomass of sedimentation mud and reach 3800～4100mg/mL, promptly realized being used to handling the screening of the microorganism species of coal chemical industrial waste water; Wherein the domestication condition in step 3, step 4 and the step 5 all is to carry out under 34～36 ℃ of conditions, first anoxia stirring 3～4h, and aeration is cultivated 14～16h then.The microorganism species that utilizes the present embodiment screening to obtain is handled coal chemical industrial waste water, result shows, the degradation rate of COD is 80%～90% in the coal chemical industrial waste water, the degradation rate of phenol is 90%～99.9%, the degradation rate of prussiate is 90%～99.9%, the degradation rate of ammonia nitrogen is 35%～50%, and the microorganism species that the present embodiment screening obtains is good to the treatment effect of coal chemical industrial waste water.
Embodiment two: present embodiment and embodiment one are different be in the step 1 under 35 ℃ of conditions aeration 14h.Other step and parameter are identical with embodiment one.
Embodiment three: that present embodiment is different with embodiment one or two is the MgSO that adds 0.5g in the step 2 in every liter of mud waste water mixture
47H
2The FeSO of O, 0.1g
47H
2The ZnSO of O, 0.01g
47H
2The MnSO of O, 0.01g
4H
2The CuSO of O, 0.05g
45H
2The NH of O, 0.25g
4The CaCl of Cl, 0.1g
2Sucrose with 3g.Other step and parameter are identical with embodiment one or two.
Embodiment four: present embodiment and embodiment three are different is that the biomass of sedimentation mud in the step 3 reaches 4000mg/mL.Other step and parameter are identical with embodiment three.
Embodiment five: present embodiment and embodiment one, two or four are different is that the COD of coal chemical industrial waste water in the step 3 is 900～1010mg/L, and phenol concentration is 800～900mg/L, and ammonia nitrogen concentration is 44～46mg/L, and the pH value is 6.9～7.1.Other step and parameter are identical with embodiment five.
The microorganism species that utilizes the inventive method screening to obtain is handled the coal chemical industrial waste water of present embodiment, result shows, the degradation rate of COD is 80%～90% in the present embodiment coal chemical industrial waste water, the degradation rate of phenol is 90%～99.9%, the degradation rate of prussiate is 90%～99.9%, the degradation rate of ammonia nitrogen is 35%～50%, and the microorganism species that the present embodiment screening obtains is good to the treatment effect of coal chemical industrial waste water.
Embodiment six: present embodiment and embodiment one, two or four are different is that the COD of coal chemical industrial waste water in the step 3 is 1080mg/L, and phenol concentration is 855mg/L, and ammonia nitrogen concentration is 45.88mg/L, and the pH value is 7.Other step and parameter are identical with embodiment one, two or four.
The microorganism species that utilizes the inventive method screening to obtain is handled the present embodiment coal chemical industrial waste water, result shows, the degradation rate of COD is 85% in the present embodiment coal chemical industrial waste water, the degradation rate of phenol is 97%, the degradation rate of prussiate is 98%, the degradation rate of ammonia nitrogen is 48%, and the microorganism species that the present embodiment screening obtains is good to the treatment effect of coal chemical industrial waste water.
Embodiment seven: the screening method that present embodiment is used for handling the microorganism species of coal chemical industrial waste water carries out according to following steps: one, the mud waste water mixture with the coal chemical industrial waste water treatment system places aeration 15h under 35 ℃ of conditions, leaves standstill 9h then to anoxic condition; Two, the MgSO that adds 0.5g in the mud waste water mixture after every liter of step 1 is handled
47H
2The FeSO of O, 0.1g
47H
2The ZnSO of O, 0.01g
47H
2The MnSO of O, 0.01g
4H
2The CuSO of O, 0.05g
45H
2The NH of O, 0.25g
4The CaCl of Cl, 0.1g
2Stir with the sucrose of 3g, aeration 15h under 35 ℃ of conditions leaves standstill and discards supernatant liquid behind the 9h and get sedimentation mud; Three, sedimentation mud is placed the coal chemical industrial waste water diluent that accounts for 4 times of sedimentation mud cumulative volumes tame, leave standstill abandoning supernatant behind the 5.5h again, promptly obtain the sedimentation mud of 1 generation domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 4 volume ratio coal chemical industrial waste water and water; Four, the sedimentation mud with the domestication of 1 generation places the coal chemical industrial waste water diluent that accounts for 4 times of sedimentation mud cumulative volumes to tame, leave standstill abandoning supernatant behind the 5.5h again, promptly obtain the sedimentation mud of 2 generations domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 2 volume ratio coal chemical industrial waste water and water; Five, the sedimentation mud of 2 generations domestications is accounted in the coal chemical industrial waste water of 3～5 times of sedimentation mud cumulative volumes and tame, tame to the biomass of sedimentation mud and reach 4000mg/mL, promptly realized being used to handling the screening of the microorganism species of coal chemical industrial waste water; Wherein the domestication condition in step 3, step 4 and the step 5 all is to carry out under 35 ℃ of conditions, first anoxia stirring 3.5h, and aeration is cultivated 15h then.
Mud waste water mixture in the present embodiment step 1 derives from the coal chemical industrial waste water treatment system of Harbin Coal Chemical Industry company limited.
Simultaneous test: first group of microorganism species processing coal chemical industrial waste water that test utilizes embodiment eight screenings to obtain; Second group of experiment, the efficient phenol degradation bacteria that adopts existing screening method to obtain is handled coal chemical industrial waste water; Wherein the water conservancy residence time of these two groups experiments are 24h, and COD is that the concentration of 1180mg/L, phenol is that the concentration of 855mg/L, prussiate is that the concentration of 35mg/L, ammonia nitrogen is that 40mg/L, pH value are 7.0 in the coal chemical industrial waste water.
Experimental result shows: the degradation rate of COD is 85% in first group of test coal chemical industrial waste water, and the degradation rate of phenol is 99.9%, and the degradation rate of prussiate is 98%, the degradation rate of ammonia nitrogen is 42%; The degradation rate of COD is 30% in second group of experiment coal chemical industrial waste water, the degradation rate of phenol is 65%, the degradation rate of prussiate is 55%, the degradation rate of ammonia nitrogen is 5%.
From experimental result as can be seen, the microorganism species that obtains of present embodiment screening method is good to the treatment effect of coal chemical industrial waste water.
Claims (6)
1. be used to handle the screening method of the microorganism species of coal chemical industrial waste water, the screening method that it is characterized in that being used for handling the microorganism species of coal chemical industrial waste water carries out according to following steps: one, the mud waste water mixture with the coal chemical industrial waste water treatment system places aeration 13～16h under 34～36 ℃ of conditions, leaves standstill 8～11h then; Two, the MgSO that adds 0.4～0.6g in the mud waste water mixture after every liter of step 1 is handled
47H
2The FeSO of O, 0.05～0.15g
47H
2The ZnSO of O, 0.005～0.015g
47H
2The MnSO of O, 0.005～0.015g
4H
2The CuSO of O, 0.005～0.015g
45H
2The NH of O, 0.2～0.3g
4The CaCl of Cl, 0.05～0.15g
2Stir with the sucrose of 2～4g, aeration 14～16h under 34～36 ℃ of conditions leaves standstill and discards supernatant liquid behind 8～10h and get sedimentation mud; Three, sedimentation mud is placed the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 1 generation domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 4 volume ratio coal chemical industrial waste water and water; Four, the sedimentation mud with the domestication of 1 generation places the coal chemical industrial waste water diluent that accounts for 3～5 times of sedimentation mud cumulative volumes to tame, leave standstill abandoning supernatant behind 5～6h again, promptly obtain the sedimentation mud of 2 generations domestication, wherein, the coal chemical industrial waste water diluent is made up of according to 1: 2 volume ratio coal chemical industrial waste water and water; Five, the sedimentation mud of 2 generations domestications is accounted in the coal chemical industrial waste water of 3～5 times of sedimentation mud cumulative volumes and tame, tame to the biomass of sedimentation mud and reach 3800～4100mg/mL, promptly realized being used to handling the screening of the microorganism species of coal chemical industrial waste water; Wherein the domestication condition in step 3, step 4 and the step 5 all is to carry out under 34～36 ℃ of conditions, first anoxia stirring 3～4h, and aeration is cultivated 14～16h then.
2. the screening method that is used to handle the microorganism species of coal chemical industrial waste water according to claim 1 is characterized in that in the step 1 aeration 14h under 35 ℃ of conditions.
3. the screening method that is used to handle the microorganism species of coal chemical industrial waste water according to claim 1 and 2 is characterized in that adding in the step 2 MgSO of 0.5g in every liter of mud waste water mixture
47H
2The FeSO of O, 0.1g
47H
2The ZnSO of O, 0.01g
47H
2The MnSO of O, 0.01g
4H
2The CuSO of O, 0.05g
45H
2The NH of O, 0.25g
4The CaCl of Cl, 0.1g
2Sucrose with 3g.
4. the screening method that is used to handle the microorganism species of coal chemical industrial waste water according to claim 3 is characterized in that the biomass of sedimentation mud in the step 3 reaches 4000mg/mL.
5. according to claim 1, the 2 or 4 described screening methods that are used to handle the microorganism species of coal chemical industrial waste water, the COD that it is characterized in that coal chemical industrial waste water in the step 3 is 900～1010mg/L, phenol concentration is 800～900mg/L, and ammonia nitrogen concentration is 44～46mg/L, and the pH value is 6.9～7.1.
6. according to claim 1, the 2 or 4 described screening methods that are used to handle the microorganism species of coal chemical industrial waste water, the COD that it is characterized in that coal chemical industrial waste water in the step 3 is 1080mg/L, and phenol concentration is 855mg/L, and ammonia nitrogen concentration is 45.88mg/L, and the pH value is 7.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070277A (en) * | 2010-11-15 | 2011-05-25 | 北京国能普华环保工程技术有限公司 | Coal gasification wastewater treatment process |
| CN102992351A (en) * | 2012-11-05 | 2013-03-27 | 青岛科技大学 | Method and device for purifying ammonia recovered from coal chemical industry wastewater |
| CN104355403A (en) * | 2014-11-27 | 2015-02-18 | 新疆环境工程技术有限责任公司 | Activated sludge culture acclimatization method for chemical industry wastewater treatment |
| CN104480037A (en) * | 2014-11-21 | 2015-04-01 | 沈阳大学 | Method for screening phthalate degradation functional floras based on Biolog technique |
| CN106544274A (en) * | 2016-10-27 | 2017-03-29 | 辽宁石油化工大学 | A kind of simple method for screening of the microorganism species of degradable 0# diesel oil |
| CN113416646A (en) * | 2021-06-21 | 2021-09-21 | 烟台南山学院 | Screening method of microbial flora for treating chemical wastewater |
-
2009
- 2009-10-26 CN CN200910073092A patent/CN101696445A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070277A (en) * | 2010-11-15 | 2011-05-25 | 北京国能普华环保工程技术有限公司 | Coal gasification wastewater treatment process |
| CN102070277B (en) * | 2010-11-15 | 2012-10-10 | 北京国能普华环保工程技术有限公司 | Coal gasification wastewater treatment process |
| CN102992351A (en) * | 2012-11-05 | 2013-03-27 | 青岛科技大学 | Method and device for purifying ammonia recovered from coal chemical industry wastewater |
| CN104480037A (en) * | 2014-11-21 | 2015-04-01 | 沈阳大学 | Method for screening phthalate degradation functional floras based on Biolog technique |
| CN104355403A (en) * | 2014-11-27 | 2015-02-18 | 新疆环境工程技术有限责任公司 | Activated sludge culture acclimatization method for chemical industry wastewater treatment |
| CN104355403B (en) * | 2014-11-27 | 2016-01-06 | 新疆环境工程技术有限责任公司 | A kind of activated sludge culture method for chemical wastewater treatment |
| CN106544274A (en) * | 2016-10-27 | 2017-03-29 | 辽宁石油化工大学 | A kind of simple method for screening of the microorganism species of degradable 0# diesel oil |
| CN113416646A (en) * | 2021-06-21 | 2021-09-21 | 烟台南山学院 | Screening method of microbial flora for treating chemical wastewater |
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Open date: 20100421 |