CN101693916A - Card for fast testing residual pesticide - Google Patents
Card for fast testing residual pesticide Download PDFInfo
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- CN101693916A CN101693916A CN200910309308A CN200910309308A CN101693916A CN 101693916 A CN101693916 A CN 101693916A CN 200910309308 A CN200910309308 A CN 200910309308A CN 200910309308 A CN200910309308 A CN 200910309308A CN 101693916 A CN101693916 A CN 101693916A
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- enzyme bar
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Abstract
A card for fast testing residual pesticide belongs to the technical field of biological engineering, and comprises two layers of plastic plates, wherein one of the plastic plates is provided with a sampling hole and an observation hole, one face of the plastic plate is provided with water-absorbing paper and an enzyme bar for fixing drosophila melanogaster acetylcholine esterase, the water-absorbing paper opposites the sampling hole, the enzyme bar opposites the observation hole, and the water-absorbing paper is connected with the enzyme bar. A base sheet for fixing indoxyl acetic ester is bonded on the other plastic plate at the position corresponding to the enzyme bar, and a baffle is arranged between the base sheet and the enzyme bar. When the sample and the indoxyl acetic ester in the enzyme bar pre-react, the baffle is drawn out to make the enzyme bar and the base sheet contact. The card for fast testing residual pesticide has the advantages of wide application range, delicate testing and fast and simple operation.
Description
Technical field
The present invention relates to a kind of quick measuring card of technical field of bioengineering, specifically is a kind of quick measuring card that detects remains of pesticide.
Background technology
China is that the first in the world agricultural chemicals uses big country and big producing country, and wherein organophosphorus and carbamate chemicals for agriculture account for 70% of high drugs kind.The pesticide residue situation is very serious in the agricultural-food such as the main vegetables of China, fruit.Simultaneously, these riskiest pesticides run off because of improper use, drift, and ecotopes such as water body, soil have been caused severe contamination.Therefore, press in China's food safety supervision and the environmental monitoring fast, the detection method and the auxiliary products of sensitivity, pesticide residue and pollution accurately.With enzyme inhibition method is that the test strip detection method of principle is as China country legal examination criteria (GB/T 5009.199), widespread use in the Fast Determination of Pesticide Residue in agricultural-food such as vegetables, fruit.But do not see report at the quick detection test paper bar of pesticidal contamination in the environment.
Find through document and patent retrieval prior art, Nagatani etc. are in " Food Control " (food control) 2007, " fast, sensitive, the disposable chip of visible is used for Fast Determination of Pesticide Residue " literary composition of delivering on 18:914～920, disclosed following content in the literary composition: utilize film as carrier matrix, behind immobilized enzyme, the developer etc., the rapid detection film chip of preparing.Yet, in the prior art, defectives such as test strip, quick measuring card and the film chip etc. of detection pesticide residue exist needs comparatively precise dose control, rely on artificial folding enzymes sheet and substrate sheet, reaction is insufficient, detection sensitivity is low can't really realize quick, sensitive, easy, detection accurately.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of quick measuring card that detects remains of pesticide is provided.Test card of the present invention is applied widely, detects sensitively, can be applied to the detection of pesticidal contamination in the environment such as pesticide residue in the agricultural-food such as vegetables, fruit and water body, soil fast, easily.
The present invention is achieved by the following technical solutions,
The present invention includes two layers of plastic; Two holes are arranged on one of them plastic plate, and one is well, and one is vision slit, one side at this plastic plate is pasted with thieving paper and the enzyme bar that is fixed with the drosophila melanogaster acetylcholinesterase, thieving paper is relative with well, and the enzyme bar is relative with vision slit, and thieving paper is connected with the enzyme bar; Be pasted with the fixedly substrate sheet of indoxyl acetate with the corresponding position of enzyme bar on another plastic plate, substrate sheet and enzyme bar and between a baffle plate is arranged; The amount of described enzyme bar fixed drosophila melanogaster acetylcholinesterase is 3～5U.
The diameter in described hole is 0.5～1cm.
Described thieving paper is of a size of: long 20mm * wide 20mm.
Described enzyme bar is of a size of: long 20mm * wide 12mm.
The amount of described substrate sheet fixed indoxyl acetate is 5～10mg.
Described substrate sheet is of a size of: long 20mm * wide 8mm.
Described baffle plate is an oiliness paraffin paper.
Described stickup is specially: the functional quality mark is that 0.4～1.2% gelatin is pasted.
The present invention at first adds testing sample solution in use in well, reaction; Extract baffle plate then, observe the color of enzyme bar, if the enzyme bar is sky blue, then non agricultural chemical residuum in the sample solution if the enzyme bar is colourless, then has pesticide residue in the sample solution.
Compared with prior art, the present invention has following beneficial effect: test card of the present invention is applied widely, detect sensitive, can be applied to the detection of pesticidal contamination in the environment such as pesticide residue in the agricultural-food such as vegetables, fruit and water body, soil fast, easily, detection time is about 10min; Simultaneously, quick measuring card of the present invention has better anti-jamming capability to complex matrices in food and the environmental sample.
Description of drawings
Fig. 1 is the structural representation of embodiment 1 quick measuring card.
Embodiment
Following example will the invention will be further described in conjunction with the accompanying drawings.Present embodiment has provided detailed embodiment and process being to implement under the prerequisite with the technical solution of the present invention, but protection scope of the present invention is not limited to following embodiment.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
Embodiment 1
Fig. 1 is the structural representation of the quick measuring card of present embodiment, as shown in Figure 1,
The structure of the quick measuring card of present embodiment is: this quick measuring card comprises two layers of plastic; Two holes are arranged on one of them plastic plate 7, and one is that well 1, is a vision slit 2, and the diameter in hole is 0.5cm;
One side at this plastic plate is pasted with thieving paper 5 and the enzyme bar 4 that is fixed with the drosophila melanogaster acetylcholinesterase, thieving paper is of a size of: long 20mm * wide 20mm, the enzyme bar is of a size of: long 20mm * wide 12mm, the amount of enzyme bar fixed drosophila melanogaster acetylcholinesterase is 3U, and the functional quality mark is that 0.4～1.2% gelatin (gelatin is water-soluble formulated) is pasted; Thieving paper 5 is relative with well 1, and enzyme bar 4 is relative with vision slit 2, and thieving paper 5 is connected with enzyme bar 4;
Be pasted with the fixedly substrate sheet 6 of indoxyl acetate with the corresponding position of enzyme bar on another plastic plate 8, substrate sheet 6 is of a size of: long 20mm * wide 8mm, and the amount of substrate sheet fixed indoxyl acetate is 5mg; Substrate sheet and enzyme bar and between an oiliness paraffin paper 3 is arranged.
Use the process of remains of pesticide in the test card test sample as follows:
Step 1, preparation blank sample liquid, described blank sample liquid is 0.1mol/L PBK, its concrete process for preparation is as follows: get the 12.48g dipotassium hydrogen phosphate, the 3.85g potassium primary phosphate, the distilled water dissolving is settled to 1L, and regulating the pH value is 7.2, gets the 0.1mol/L PBK of 1L;
Step 2 adds 0.5mL blank sample liquid in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the colour developing result in the vision slit as a result after 5 minutes.The result shows, presents tangible sky blue in the vision slit, i.e. non agricultural chemical residuum in the sample liquid;
Step 3 adds 0.5mL 0.3mg/L SD-1750 (what dilute employing is the PBK solution of step 1) in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the result that develops the color in the vision slit as a result after 5 minutes.The result shows, presents colourlessly in the vision slit, promptly in the sample liquid pesticide residue arranged.
Embodiment 2
The quick measuring card and the embodiment of present embodiment have identical construction, and difference is: the diameter of well and vision slit is 0.7cm; The amount of enzyme bar fixed drosophila melanogaster acetylcholinesterase is 4U; The amount of substrate sheet fixed indoxyl acetate is 8mg; Stickup is to use 0.7% gelatin to carry out.
Use the process of remains of pesticide in the test card test sample as follows:
Step 1, preparation blank sample liquid, described blank sample liquid is 0.1mol/L PBK, its concrete process for preparation is as follows: get the 12.48g dipotassium hydrogen phosphate, the 3.85g potassium primary phosphate, the distilled water dissolving is settled to 1L, and regulating the pH value is 7.2, gets the 0.1mol/L PBK of 1L;
Step 2 adds 0.5mL blank sample liquid in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the colour developing result in the vision slit as a result after 5 minutes.The result shows, presents tangible sky blue in the vision slit, i.e. non agricultural chemical residuum in the sample liquid;
Step 3 adds 0.5mL 0.5mg/L Trichlorphon (what dilute employing is the PBK solution of step 1) in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the result that develops the color in the vision slit as a result after 5 minutes.The result shows, presents colourlessly in the vision slit, promptly in the sample liquid pesticide residue arranged.
Embodiment 3
The quick measuring card and the embodiment of present embodiment have identical construction, and difference is: the diameter of well and vision slit is 1cm; The amount of enzyme bar fixed drosophila melanogaster acetylcholinesterase is 5U; The amount of substrate sheet fixed indoxyl acetate is 10mg; Stickup is to use 1.2% gelatin to carry out.
Use the process of remains of pesticide in the test card test sample as follows:
Step 1, preparation blank sample liquid, described blank sample liquid is 0.1mol/L PBK, its concrete process for preparation is as follows: get the 12.48g dipotassium hydrogen phosphate, the 3.85g potassium primary phosphate, the distilled water dissolving is settled to 1L, and regulating the pH value is 7.2, gets the 0.1mol/L PBK of 1L;
Step 2 adds 0.5mL blank sample liquid in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the colour developing result in the vision slit as a result after 5 minutes.The result shows, presents tangible sky blue in the vision slit, i.e. non agricultural chemical residuum in the sample liquid;
Step 3 adds 0.5mL 0.8mg/L omethoate (what dilute employing is the PBK solution of step 1) in well, room temperature condition reacted 6 minutes down, took out baffle plate, is observing the result that develops the color in the vision slit as a result after 5 minutes.The result shows, presents colourlessly in the vision slit, promptly in the sample liquid pesticide residue arranged.
Claims (8)
1. a quick measuring card that detects remains of pesticide is characterized in that, this quick measuring card comprises two layers of plastic; Two holes are arranged on one of them plastic plate, and one is well, and one is vision slit, one side at this plastic plate is pasted with thieving paper and the enzyme bar that is fixed with the drosophila melanogaster acetylcholinesterase, thieving paper is relative with well, and the enzyme bar is relative with vision slit, and thieving paper is connected with the enzyme bar; Be pasted with the fixedly substrate sheet of indoxyl acetate with the corresponding position of enzyme bar on another plastic plate, substrate sheet and enzyme bar and between a baffle plate is arranged; The amount of described enzyme bar fixed drosophila melanogaster acetylcholinesterase is 3～5U.
2. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, the diameter in described hole is 0.5～1cm.
3. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, described thieving paper is of a size of: long 20mm * wide 20mm.
4. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, described enzyme bar is of a size of: long 20mm * wide 12mm.
5. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, described substrate sheet is of a size of: long 20mm * wide 8mm.
6. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, the amount of described substrate sheet fixed indoxyl acetate is 5～10mg.
7. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, described baffle plate is an oiliness paraffin paper.
8. the quick measuring card of detection remains of pesticide according to claim 1 is characterized in that, described stickup is specially: the functional quality mark is that 0.4～1.2% gelatin is pasted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009103093083A CN101693916B (en) | 2009-11-05 | 2009-11-05 | Card for fast testing residual pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009103093083A CN101693916B (en) | 2009-11-05 | 2009-11-05 | Card for fast testing residual pesticide |
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| Publication Number | Publication Date |
|---|---|
| CN101693916A true CN101693916A (en) | 2010-04-14 |
| CN101693916B CN101693916B (en) | 2011-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009103093083A Expired - Fee Related CN101693916B (en) | 2009-11-05 | 2009-11-05 | Card for fast testing residual pesticide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399850A (en) * | 2010-09-16 | 2012-04-04 | 上海市农业科学院 | Rapid detection card for detecting pesticide residues, preparation method and application thereof |
| CN102735830A (en) * | 2012-07-04 | 2012-10-17 | 浙江农林大学 | Preparation method for ethyl urethane detection test paper and detection method of test paper |
| CN104655621A (en) * | 2015-02-03 | 2015-05-27 | 广州绿洲生化科技股份有限公司 | Fast testing card for organophosphorus and carbamate pesticide residues |
| CN105928929A (en) * | 2016-04-20 | 2016-09-07 | 郑州大学 | Rapid detection point card for pesticide residues and method for detecting content of pesticide residues by using detection point card |
| CN106568890A (en) * | 2016-11-07 | 2017-04-19 | 吴文华 | Portable pesticide residue detection device |
| CN107746838A (en) * | 2017-08-21 | 2018-03-02 | 郑州炜盛电子科技有限公司 | Detecting Pesticide enzyme source and its application |
| CN108872224A (en) * | 2018-07-13 | 2018-11-23 | 云南商测质量检验技术服务有限公司 | A kind of Fast Determination of Pesticide Residue ball and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398426B (en) * | 2018-03-15 | 2021-03-23 | 鲁东大学 | Simple pesticide residue rapid detection device and application thereof |
-
2009
- 2009-11-05 CN CN2009103093083A patent/CN101693916B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399850A (en) * | 2010-09-16 | 2012-04-04 | 上海市农业科学院 | Rapid detection card for detecting pesticide residues, preparation method and application thereof |
| CN102735830A (en) * | 2012-07-04 | 2012-10-17 | 浙江农林大学 | Preparation method for ethyl urethane detection test paper and detection method of test paper |
| CN104655621A (en) * | 2015-02-03 | 2015-05-27 | 广州绿洲生化科技股份有限公司 | Fast testing card for organophosphorus and carbamate pesticide residues |
| CN105928929A (en) * | 2016-04-20 | 2016-09-07 | 郑州大学 | Rapid detection point card for pesticide residues and method for detecting content of pesticide residues by using detection point card |
| CN106568890A (en) * | 2016-11-07 | 2017-04-19 | 吴文华 | Portable pesticide residue detection device |
| CN107746838A (en) * | 2017-08-21 | 2018-03-02 | 郑州炜盛电子科技有限公司 | Detecting Pesticide enzyme source and its application |
| CN108872224A (en) * | 2018-07-13 | 2018-11-23 | 云南商测质量检验技术服务有限公司 | A kind of Fast Determination of Pesticide Residue ball and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101693916B (en) | 2011-08-31 |
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