CN101691539A - Filamentous fungi fermented for producing ethanol by xylose and preparation method thereof - Google Patents

Filamentous fungi fermented for producing ethanol by xylose and preparation method thereof Download PDF

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CN101691539A
CN101691539A CN200910071929A CN200910071929A CN101691539A CN 101691539 A CN101691539 A CN 101691539A CN 200910071929 A CN200910071929 A CN 200910071929A CN 200910071929 A CN200910071929 A CN 200910071929A CN 101691539 A CN101691539 A CN 101691539A
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wood
fermentation
ethanol
sugar
fusarium
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杨谦
范金霞
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Harbin Institute of Technology
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Harbin Institute of Technology
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The invention provides filamentous fungi fermented for producing ethanol by xylose and a preparation method thereof. The microorganism of Fusarium xysporum CS-28 is collected in the China Center for Type Culture Collection (CCTCC) on March 11, 2009 and has the collection registration number of CCTCC: M209040. The filamentous fungi can be directly fermented for producing the ethanol by the xylose, which particularly provides a good way of making full use of the fermentable sugar whose cellulose is hydrolyzed. The filamentous fungi which is the Fusarium xysporum CS-28 can be fermented for producing the ethanol by the raw material of the xylose, and the best nitrogen source is yeast extract, in particular the soybean meal which is equivalent to the yeast extract in the output of the ethanol, thus the cost of the raw material is reduced. In addition, the filamentous fungi is cultured in a static way in a semi-aerobic state, thus the stirring process is omitted and the process cost and the production cost are recued accordingly.

Description

Utilize wood-sugar fermentation to produce alcoholic acid filamentous fungus and preparation method thereof
(1) technical field
The Environmental Biotechnology Application Areas that belongs to of the present invention is specifically related to a kind of fungi and preparation method thereof.
(2) background technology
The enhancing of the appearance of world energy sources crisis and people's environmental consciousness, biomass energy have received very big concern.Alcohol fuel has the characteristics of environment friendly because its pollution is little, becomes the New-type fuel resource and is expected to replace the fossil energy (as oil and coal) that reduces day by day.Tradition ethanol fermentation raw material is food crop such as corn, and today of food shortage, this mode of production was subjected to great restriction.Lignocellulose material (as agricultural waste material, wood chip, potato slag etc.) is the abundantest in the world biomass resource, is made up of Mierocrystalline cellulose, hemicellulose and xylogen, and its hydrolysate mainly is glucose and wood sugar.Yeast saccharomyces cerevisiae can glucose fermentation but unfermentable wood sugar produces ethanol.Since the eighties, numerous scholars has carried out the screening and the genetically engineered research of bacterial classification of wood sugar bacterial classification, the wood-sugar fermentation microorganism mainly concentrates on pichia yeast (Pichia), candiyeast (Candida) and three genus of pipe capsule yeast (Pachysolen), making up gene recombination bacterium host's bacterial classification commonly used has yeast saccharomyces cerevisiae (S.cerevisiae), zymomonas mobilis (Zymomonas mobilis) and intestinal bacteria (E.coli), and the wood-sugar fermentation of filamentous fungus is studied seldom.
(3) summary of the invention
The object of the present invention is to provide a kind of raw materials cost low, and training method is that agitating procedure has been saved in static cultivation (half good oxygen condition), reduced the technology cost, thereby the wood-sugar fermentation that utilizes that has reduced production cost produces alcoholic acid filamentous fungus and preparation method thereof.
Microorganism Fusarium oxysporum Fusarium xysporum CS-28 of the present invention was preserved in " Chinese typical culture collection " center "; preservation registration number is: CCTCC:M209040; the bacterial strain of called after Fusarium oxysporum CS-28 (Fusarium xysporum CS-28); belong to mycota (Fungi); Ascomycota (Ascimycota); cup fungi subphylum (Pezizomycotina) on 03 16th, 2009, excrement shell Gammaproteobacteria (Sordariomycetes), Hypocreales (Hypocreales), mitotic division fungi Hypocreaceae (Mitosporic Hypocreales), Fusarium (Fusarium).
Few at utilizing the wood-sugar fermentation bacterial classification at present, the present situation that alcohol yied is not high, the present invention screens the bacterial strain of a strain filamentous fungus xylose fermentation ethanol from occurring in nature, it morphology and molecular biology identification have been carried out, simultaneously its fermentation condition being optimized, is that a strain utilizes wood-sugar fermentation to produce the ethanol filamentous fungus.Its morphological characteristic is:
In conjunction with Fig. 1, Fig. 2, this bacterial strain colony diameter after cultivating 2d on the PDA substratum surpasses 2.5cm, and 7d reaches 8-9cm; Under room temperature (25 ℃), mycelial growth is good, and flocculence is to the fine hair shape, initial white, and central gradually pink colour, the later stage is changed purple into, substratum reverse side intense violet color; In conjunction with Fig. 3, Fig. 4, mycelia dyeing back microscopic examination result, mycelia has every, branch, transparent; Conidium spindle-sickleshaped, the two ends gradual change is narrow, and base portion does not have the pin spore, have every, have the size two types of conidiums, microconidium quantity is more, the phialide that singly goes out from the adnation of mycelia grows or grows from conidiophore, is not that chain is given birth to but false head and given birth to; Ellipse, fusiform, avette, comma shape; Macroconidium is less often, originates in the pionnote or from a minute sporophore to grow; Sickleshaped, fusiform; The apical cell is narrow thin.
Through molecular biology identification: district between the 18s rDNA of Fusarium oxysporum Fusarium xysporum CS-28 transcribes (Internaltranscribedspacer, sequence results ITS):
1 TGATATGCTT?AAGTTCAGCG?GGTATTCCTA?CCTGATCCGA?GGTCAACATT?CAGAAGTTGG
61 GGGTTTAACG?GCTTGGCCGC?GCCGCGTTCC?AGTTGCGAGG?GTTTTACTAC?TACGCAATGG
121 AGGCTGCAGC?GAGACCGCCA?CTAGATTTCG?GGGCCGGCTT?GCCGCAAGGG?CTCGCCGATC
181 CCCAACACCA?AACCCGAGGG?CTTGAGGGTT?GAAATGACGC?TCGAACAGGC?ATGCCCGCCA
241 GAATACTGGC?GGGCGCAATG?TGCGTTCAAA?GATTCGATGA?TTCACTGAAT?TCTGCAATTC
301 ACATTACTTA?TCGCATTTTG?CTGCGTTCTT?CATCGATGCC?AGAACCAAGA?GATCCGTTGT
361 TGAAAGTTTT?GATTTATTTA?TGGTTTTACT?CAGAAGTTAC?ATATAGAAAC?AGAGTTTAGG
421 GGTCCTCTGG?CGGGCCGTCC?CGTTTTACCG?GGAGCGGGCT?GATCCGCCGA?GGCAACAAGT
481 GGTATGTTCA?CAGGGGTTTG?GGAGTTGTAA?ACTCGGTAAT?GATCCCTCCG?CAGGT
Growth characteristics: thalline is less demanding to substratum, and pH can grow naturally on the PDA substratum, and culture temperature is at 20-30 ℃.
Metabolic characteristics:
1) different carbon sources utilize situation:
Bacterial strain can be with wood sugar, fructose, glucose, semi-lactosi, maltose, sucrose, maize straw, starch, Xylo-Mucine, Xylitol, N.F,USP MANNITOL as the carbon source generation ethanol that ferments.Bacterial classification is the strongest to the zymamsis ability of sucrose, glucose, starch, semi-lactosi, and is the most weak to the fermentation capacity of lactose.Sickle-like bacteria all has in various degree zymamsis ability to 12 kinds of carbon sources, and its carbon source spectrum of use is extensive, is expected to become industrial fermentation bacterial classification to be selected.
2) thalline utilizes situation to nitrogenous source:
Select yeast extract respectively for use, peptone, bean flour, Semen Maydis powder, soya-bean cake water, dregs of beans, urine, (NH 4) 2SO 4, KNO 3, NH 4NO 3, NaNO 3, NH 4AC carries out fermentation test with wood sugar as carbon source as nitrogenous source, the results are shown in Figure 6: different nitrogen sources shows the test-results of Fusarium oxysporum fermentation producing and ethanol: organic nitrogen source is than the amount height of inorganic nitrogen-sourced producing and ethanol.The producing and ethanol amount of soy noodle is 4.28ml/L in organic nitrogen source, maintains an equal level with yeast extract (yeast extract) ferment effect (4.25ml/l), considers fermentation costs, adopts soy noodle as nitrogenous source in the following fermentation test.In inorganic nitrogen-sourced, nitric nitrogen is more effective than ammonium nitrogen, wherein NaNO 3Fermentation efficiency the highest, reached 2.61ml/L.
It can also be seen that from Fig. 6: wood-sugar fermentation time ethanol content in the time of the 6th day is best, begins during by the 7th day to descend.
Fermentation character: in conjunction with Fig. 7,
1) influence of initial pH value to fermenting: initial pH value is very big to the influence of fermentation, and initial pH value is made as 5.4,5.6 respectively, 5.8,6.0,6.2, along with pH is worth the rising ethanol production in rising trend, ethanol production is up to 5.51ml/l during pH 6.0, sharply descends again subsequently.
2) in conjunction with Fig. 8, the influence of temperature to fermenting: temperature is very big to the influence of ethanol fermentation, and temperature is too high can to increase the alcoholic acid evaporation, and temperature is crossed to hang down influences thalli growth, thereby influences ethanol production, and test-results shows: temperature is that 30 ℃ of ethanol productions are the highest.Various substratum:
Substratum is preserved on the inclined-plane: every liter of nutrient solution contains wood sugar 20g, agar 20g, yeast extract 10g, peptone 5g, pH nature;
The activated liquid substratum: every liter of nutrient solution contains wood sugar 10g, potato 200g, pH value nature;
The activating solid substratum: every liter of nutrient solution contains wood sugar 10g, potato 200g, agar 20g, pH value nature;
Seed culture medium: every L nutrient solution contains wood sugar 10g, CaCl 20.1g, MgSO 40.2g, KH 2PO 42g, nitrogenous source is decided according to test
Fermention medium: every L nutrient solution contains CaCl 20.1g, MgSO4 0.2g, KH 2PO 42g, carbon source and nitrogenous source are decided according to test.
Method:
Cultural method: from slant medium, provoke in a small amount of mycelia and the activating solid substratum, cultivated 7 days for 30 ℃, wash spore with sterilized water, by 1 * 10 6Individual spore is inoculated in the 100ml that the 20ml seed culture fluid is housed for every liter and shakes in the bottle, and 30 ℃ of 200rpm cultivated 2 days.
Fermentation process: 10% inoculum size be inoculated in the 100ml that the 50ml fermention medium is housed shake bottle in, static cultivation a couple of days, carry out the mensuration of ethanol content.
Advantage of the present invention and effect have:
Filamentous fungus of the present invention can directly utilize wood-sugar fermentation to produce ethanol, belongs to the Environmental Biotechnology Application Areas, especially making full use of of the fermentable saccharide behind the cellulose hydrolysis is improved good approach.Filamentous fungus Fusarium oxysporum of the present invention is the raw material generation ethanol that ferments with the wood sugar, best nitrogenous source is a yeast extract, but best nitrogenous source of the present invention is selected the soy noodle suitable with its ethanol production, greatly reduce raw materials cost, and training method is that agitating procedure has been saved in static cultivation (half good oxygen condition), reduce the technology cost, thereby reduced production cost.
Bacterial classification of the present invention has the generation cellulase simultaneously, hemicellulase, and the characteristics of plurality of enzymes such as amylase system can be utilized maize straw, and starch and Xylo-Mucine are that carbon source is fermented, and can realize synchronous glycosylation and fermenting process.It can not only fermented cellulose etc. polymkeric substance, multiple monose (as glucose, semi-lactosi etc.) only utilizes single culture can realize the purpose of degraded cellulose fermentative production of ethanol simultaneously.Can improve utilization ratio by further condition of enzyme production optimization, transform and improve new bacterial classification for realizing utilizing the cellulose wood raw material to carry out the energy early to stalk.
(4) description of drawings
Fig. 1-Fig. 2 is the colonial morphology synoptic diagram that Fusarium oxysporum grew 7 days in the PDA substratum;
Fig. 3-Fig. 4 is the microscopic examination synoptic diagram of spore shape;
Fig. 5 is that different carbon sources influence synoptic diagram to the Fusarium oxysporum fermentation;
Fig. 6 is the influence synoptic diagram of different nitrogen sources to the Fusarium oxysporum fermentation;
Fig. 7 is the influence synoptic diagram of initial pH value to fermentation;
Fig. 8 is the influence synoptic diagram of temperature to fermentation.
(5) embodiment
The present invention is further illustrated below in conjunction with specific embodiment:
Embodiment 1: present embodiment adopts:
Seed culture medium: every L nutrient solution contains wood sugar 10g, soy noodle 10g, NaNO 32g, CaCl 20.1g, MgSO 40.2g, KH 2PO 42g, pH value 6.0;
Fermention medium: every L nutrient solution contains wood sugar 20g, soy noodle 10g, NaNO 32g, CaCl 20.1g, MgSO4 0.2g, KH 2PO 42g, pH value 6.0;
Cultural method: from slant medium, provoke in a small amount of mycelia and the activating solid substratum, cultivated 7 days for 30 ℃, wash spore with sterilized water, by 1 * 10 6Individual spore is inoculated in the 100ml that the 20ml seed culture fluid is housed for every liter and shakes in the bottle, and 30 ℃ of 200rpm cultivated 2 days.
Fermentation process: 10% inoculum size be inoculated in the 100ml that the 50ml fermention medium is housed shake bottle in, the mensuration of ethanol content is carried out in static cultivation 6 days.
Use above-mentioned substratum and method and carry out the wood-sugar fermentation test, in 2% wood-sugar fermentation substratum, the alcoholic acid peak concentration is 5.89g/L, and output is 0.295g/g, reaches 57.84% of theoretical yield (0.51g/g).
Embodiment 2: soil sample is picked up from the maize straw that the Harbin cottage area was piled up 2 years, and E.coli Top10 preserves for the contriver laboratory.
Reagent:
Taq archaeal dna polymerase, pMD-18T support agent box are precious biotechnology (Dalian) company limited product; It is Shanghai China Shun biotech firm that glue reclaims test kit, and primer synthesizes and order-checking is finished by the living worker in Shanghai Bioisystech Co., Ltd; Wood sugar is available from U.S. Sigma company; Other reagent is homemade analytical reagent.
Substratum:
Primary dcreening operation substratum (PXA): potato 200g/L, wood sugar 20g/L, agar 20g/L, pH value 6.0.
Sieve substratum again: wood sugar 1%, yeast powder 0.4%, peptone 0.2%, (NH 4) 2SO 40.6%, KH 2PO 40.1%, MgSO 47H 2O 0.1%, CaCl 20.5%.
Method: strain separating screening:
The soil sample of getting the 1g collection is dissolved in 30 ℃ of vibration 1h in the 10mL sterilized water, after suitably diluting, coats on the PXA flat board 30 ℃ of constant temperature culture 48h; The picking very fast bacterium colony of growing, through the separating for several times purifying, the filamentous fungus pure growth of acquisition is preserved.
Single bacterium colony behind the purifying carries out the fermentation test of Du Shi pipe through multiple screening culture medium and further screens, and utilizes fermention medium to carry out the mensuration of ethanol content at last, picks out the highest bacterial strain of ethanol content as next step research object.
Morphology is identified with reference to " fungi identification handbook ".
18S rDNA primer: T1:5 '-TCC GTA GGT GAA CCT GCG G-3 '
14：5’-TCC?TCC?GCT?TAT?TGA?TAT?GC-3’
The PCR reaction system: all PCR reactions are all carried out in 20 μ L standard reaction systems, wherein contain 2 μ L Buffer damping fluids, 1.6 μ 2.5mMdNTPs, each primer is 1uL (10mM), 0.125uLTaq enzyme, 1 μ L template DNA adds aseptic ultrapure water to 20 μ L, on the PCR instrument, press 94 ℃ of pre-sex change 5min, press 94 ℃, 30s, 56 ℃, 30s, 72 ℃, 1min then, circulate 32 times, last 72 ℃ are extended 10min.
The result: bacterial strain colony diameter after cultivating 4d on the PDA substratum surpasses 2.5cm, and 10d reaches 6～7cm; Under room temperature (26 ℃), mycelial growth is good, and flocculence is to the fine hair shape, initial white, and central gradually pink colour, the later stage is changed purple into, substratum reverse side intense violet color; Mycelia has every, branch, and is transparent, and diameter 1.5～3.5 μ m produce.
Have size to divide steamed stuffed bun: microconidium quantity is more, and the phialide that singly goes out from the adnation of mycelia grows or grows from conidiophore, is not that chain is given birth to but false head and given birth to; Ellipse, fusiform, avette, comma shape; Tool 0～1 every, transparent, smooth; Macroconidium is less often, originates in the pionnote or from conidiophore to grow; Sickleshaped, fusiform; The apical cell is narrow thin, slightly point; Sporoderm is thin; Most 3 separate, and are 5 separations less.Chlamydospore is born in the middle of the mycelia or the top, and list is given birth to or concatenated, and is spherical to avette, smooth, and diameter 5～10 μ m form more sparse on mycelia.Qualification result is: Fusarium oxysporum.
Sequence table: district between the 18s rDNA of Fusarium oxysporum Fusarium xysporumCS-28 transcribes (Internaltranscribedspacer, sequence results ITS):
1 TGATATGCTT?AAGTTCAGCG?GGTATTCCTA?CCTGATCCGA?GGTCAACATT?CAGAAGTTGG
61 GGGTTTAACG?GCTTGGCCGC?GCCGCGTTCC?AGTTGCGAGG?GTTTTACTAC?TACGCAATGG
121 AGGCTGCAGC?GAGACCGCCA?CTAGATTTCG?GGGCCGGCTT?GCCGCAAGGG?CTCGCCGATC
181 CCCAACACCA?AACCCGAGGG?CTTGAGGGTT?GAAATGACGC?TCGAACAGGC?ATGCCCGCCA
241 GAATACTGGC?GGGCGCAATG?TGCGTTCAAA?GATTCGATGA?TTCACTGAAT?TCTGCAATTC
301 ACATTACTTA?TCGCATTTTG?CTGCGTTCTT?CATCGATGCC?AGAACCAAGA?GATCCGTTGT
361 TGAAAGTTTT?GATTTATTTA?TGGTTTTACT?CAGAAGTTAC?ATATAGAAAC?AGAGTTTAGG
421 GGTCCTCTGG?CGGGCCGTCC?CGTTTTACCG?GGAGCGGGCT?GATCCGCCGA?GGCAACAAGT
481 GGTATGTTCA?CAGGGGTTTG?GGAGTTGTAA?ACTCGGTAAT?GATCCCTCCG?CAGGT
The sequence table sample
＜110〉Harbin Institute of Technology
＜120〉utilize wood-sugar fermentation to produce alcoholic acid filamentous fungus and preparation method thereof
<160>1
<210>1
<211>536
<212>DNA
<213>Fusarium?xysporum?CS-28
<400>
TGATATGCTT?AAGTTCAGCG?GGTATTCCTA?CCTGATCCGA?GGTCAACATT?CAGAAGTTGG 1
GGGTTTAACG?GCTTGGCCGC?GCCGCGTTCC?AGTTGCGAGG?GTTTTACTAC?TACGCAATGG 61
AGGCTGCAGC?GAGACCGCCA?CTAGATTTCG?GGGCCGGCTT?GCCGCAAGGG?CTCGCCGATC 121
CCCAACACCA?AACCCGAGGG?CTTGAGGGTT?GAAATGACGC?TCGAACAGGC?ATGCCCGCCA 181
GAATACTGGC?GGGCGCAATG?TGCGTTCAAA?GATTCGATGA?TTCACTGAAT?TCTGCAATTC 241
ACATTACTTA?TCGCATTTTG?CTGCGTTCTT?CATCGATGCC?AGAACCAAGA?GATCCGTTGT 301
TGAAAGTTTT?GATTTATTTA?TGGTTTTACT?CAGAAGTTAC?ATATAGAAAC?AGAGTTTAGG 361
GGTCCTCTGG?CGGGCCGTCC?CGTTTTACCG?GGAGCGGGCT?GATCCGCCGA?GGCAACAAGT 421
GGTATGTTCA?CAGGGGTTTG?GGAGTTGTAA?ACTCGGTAAT?GATCCCTCCG?CAGGT 481

Claims (3)

1. one kind is utilized wood-sugar fermentation to produce the alcoholic acid filamentous fungus, it is characterized in that described filamentous fungus is to be preserved in " Chinese typical culture collection " center "; preservation registration number is: CCTCC:M209040, the bacterial strain of called after Fusarium oxysporum CS-28 (Fusarium xysporum CS-28) on 03 16th, 2009.
2. according to claim 1ly utilize wood-sugar fermentation to produce the alcoholic acid filamentous fungus, the district was between the 18s rDNA that it is characterized in that described Fusarium oxysporum Fusarium xysporum CS-28 transcribed:
TGATATGCTT?AAGTTCAGCG?GGTATTCCTA?CCTGATCCGA?GGTCAACATT?CAGAAGTTGG 1
GGGTTTAACG?GCTTGGCCGC?GCCGCGTTCC?AGTTGCGAGG?GTTTTACTAC?TACGCAATGG 61
AGGCTGCAGC?GAGACCGCCA?CTAGATTTCG?GGGCCGGCTT?GCCGCAAGGG?CTCGCCGATC 121
CCCAACACCA?AACCCGAGGG?CTTGAGGGTT?GAAATGACGC?TCGAACAGGC?ATGCCCGCCA 181
GAATACTGGC?GGGCGCAATG?TGCGTTCAAA?GATTCGATGA?TTCACTGAAT?TCTGCAATTC 241
ACATTACTTA?TCGCATTTTG?CTGCGTTCTT?CATCGATGCC?AGAACCAAGA?GATCCGTTGT 301
TGAAAGTTTT?GATTTATTTA?TGGTTTTACT?CAGAAGTTAC?ATATAGAAAC?AGAGTTTAGG 361
GGTCCTCTGG?CGGGCCGTCC?CGTTTTACCG?GGAGCGGGCT?GATCCGCCGA?GGCAACAAGT 421
GGTATGTTCA?CAGGGGTTTG?GGAGTTGTAA?ACTCGGTAAT?GATCCCTCCG?CAGGT 481。
3. one kind prepares the preparation method who utilizes wood-sugar fermentation to produce the alcoholic acid filamentous fungus according to claim 1, it is characterized in that it may further comprise the steps:
(1) seed culture medium: every L nutrient solution contains wood sugar 10g, soy noodle 10g, NaNO 32g, CaCl 20.1g, MgSO 40.2g, KH 2PO 42g, pH value 6.0;
(2) fermention medium: every L nutrient solution contains wood sugar 20g, soy noodle 10g, NaNO 32g, CaCl 20.1g, MgSO4 0.2g, KH 2PO 42g, pH value 6.0;
(3) cultural method: from slant medium, provoke in a small amount of mycelia and the activating solid substratum, cultivated 7 days for 30 ℃, wash spore with sterilized water, by 1 * 10 6Individual spore is inoculated in the 100ml that the 20ml seed culture fluid is housed for every liter and shakes in the bottle, and 30 ℃ of 200rpm cultivated 2 days.
(4) fermentation process: 10% inoculum size be inoculated in the 100ml that the 50ml fermention medium is housed shake bottle in, the mensuration of ethanol content is carried out in static cultivation 6 days;
(5) use above-mentioned substratum and method and carry out the wood-sugar fermentation test, in 2% wood-sugar fermentation substratum, the alcoholic acid peak concentration is 5.89g/L, obtains bacterial strain, and output is 0.295g/g.
CN200910071929A 2009-04-30 2009-04-30 Filamentous fungi fermented for producing ethanol by xylose and preparation method thereof Pending CN101691539A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10655155B2 (en) 2014-10-22 2020-05-19 University Of Strathclyde Bioprocess for coproduction of ethanol and mycoproteins
CN112522117A (en) * 2020-12-29 2021-03-19 中国科学院成都生物研究所 Chaetomium and application thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10655155B2 (en) 2014-10-22 2020-05-19 University Of Strathclyde Bioprocess for coproduction of ethanol and mycoproteins
US11293044B2 (en) 2014-10-22 2022-04-05 University Of Strathclyde Bioprocess for coproduction of ethanol and mycoproteins
CN112522117A (en) * 2020-12-29 2021-03-19 中国科学院成都生物研究所 Chaetomium and application thereof
CN112522117B (en) * 2020-12-29 2022-06-28 中国科学院成都生物研究所 Chaetomium and application thereof

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