CN101690814B - Traditional Chinese medicine monomer having function of promoting growth of motoneuron - Google Patents
Traditional Chinese medicine monomer having function of promoting growth of motoneuron Download PDFInfo
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- CN101690814B CN101690814B CN2009100354861A CN200910035486A CN101690814B CN 101690814 B CN101690814 B CN 101690814B CN 2009100354861 A CN2009100354861 A CN 2009100354861A CN 200910035486 A CN200910035486 A CN 200910035486A CN 101690814 B CN101690814 B CN 101690814B
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Abstract
The invention belongs to the research and development field of neuro-protective medicament, and discloses a traditional Chinese medicine monomer capable of promoting growth of motoneuron. In the aspect of isolated cell level, the traditional Chinese medicine compound, can obviously promote enation of motoneuron; in the aspect of integral animal level, the compound can obviously promote functional rehabilitation of impaired sciatic nerve and femoral nerve. The invention can be applied to medicinal development for amyotrophic lateral sclerosis and relevant motoneuro symptoms.
Description
Technical field:
The present invention relates to the research and development field of nerve protection medicine, particularly from Chinese medicine, searched out the Chinese medicine monomer and the derivant thereof that can be used for the treatment of damage of motoneurons and relevant disease.
Background technology:
Motor neuron is as a kind of nervous system chronic lethal degenerative disease, the method for its healing of no-trump still at present.But since finding over more than 100 year of motor neuron, people just struggle with it always, have attempted nearly hundred kinds of Drug therapys.Along with the development of neuroscience,, a series of achievements that attract people's attention have been obtained in the challenge of people to this refractory disease to the cause of disease of motor neuron and the deep understanding of pathophysiology.Current main treatment comprises etiological treatment, the Supporting Therapy of symptomatic treatment and multiple non-medicine.The developing direction that the present stage treatment is studied comprises neuroprotective, anti-exitotoxicity medicine, neurotrophic factor, antioxidation and aspects such as free radical scavenger, stem cell and gene therapy.
Motor neuron belongs to traditional Chinese medical science myopathy, and myopathy is the relax disease of weak useless usefulness of limbs muscle arteries and veins.The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, and main store essential substances, main bone are given birth to marrow.Traditional Chinese medical science myopathy, the relation of itself and kidney is the closest, the natural endowment deficiency, the damage of essence insufficiency of blood can not nutrition muscle muscles and bones, engenders muscle weakness, atrophy.Simultaneously, The spleen and stomach provide the material basis of the acquired, changes angry blood, the nutrition vital organs of the human body, muscle muscles and bones, and spleen governing muscles, and weakness of the spleen and stomach, insufficiency of generating and transforming QI and blood, muscle are difficult nutrition then amyotrophy, muscle weakness etc.
Adopt muscle sacred disease, myopathy such as the pure Chinese medicinal formulae treatment of traditional Chinese medical science motor neuron, can obtain the good curing effect.Because suitable Chinese herbal medicine is as chelating agen, can follow material chelatings such as sedimentary, too much in the tissue, organ, harmful free radicals, calcareous, lipid, collagen protein, mucopolysaccharide, lead, hydrargyrum, arsenic, aluminum, copper, drain all virulence factors of body accumulation, paathogenic factors such as the antibody in the removal patient blood plasma, immune complex have been eliminated disease; Simultaneously, chelation by chelating agen, drained big quantity of material in the body, cause intravital these the deleterious or harmless materials of machine sharply to reduce, material by these falls sharply, stimulate the nerve of body, endocrine, immunity, digestion, circulation, breathe, urinary system, cental systems such as reproduction, carry out stress, a series of endogenous material such as neurotransmitter have been produced, hormone, immune factor etc., body " is weeded out the old and bring forth the new ", effect such as " remould oneself thoroughly " is the whole human body detoxifcation, has recovered the metabolic balance of body, thereby intravital all hidden danger of the machine of having dispeled and focus have finally reached the purpose of preventing and curing diseases.At present the effective Chinese medicinal formulae of clinical report comprises and adds flavor chien pu hu chien wan, beneficial marrow soup, class BUYANG HUANWU TANG, flaccidity soup etc. more.
Summary of the invention:
Therefore the invention discloses the Chinese medicine monomer that a class has special construction, this compounds has the function that promotes the motor neuron growth, it is characterized in that: in the isolated cells level, and the promotion motor neuron enation that this compounds can significance; In the whole animal level, the impaired sciatic nerve of promotion that this compounds can significance and the functional rehabilitation of femoral nerve.
The wherein said motor neuron that is used for screening drug compound is meant with the resulting motor neuron of primitive cell culture method.
This type of reactive compound carries out the checking of integral animal horizontal functional again, it is characterized in that subordinate act and morphology, SABC three aspects are index, verifies whether this compounds can promote the sciatic nerve and the femoral nerve that damage to rejuvenate.
This type of has the Chinese medicine monomer that promotes motor neuron, it is characterized in that belong to flavone compound, its structure aglycon is a flavone, and the A ring must structure be 5, the 7-dihydroxy, and 6 and 8 can unsubstituteds, also can be hydroxyl replacement or methoxyl group replacement; The B ring can have 1-5 hydroxyl, or other substituent group; 3 on C ring is a hydrogen, as apigenin, chrysin, baicalin, 4 '-hydroxyl baicalin, luteolin, oroxylin A, diosmetin, tricin etc.
If structure aglycon flavonol, A ring must structure be 5, the 7-dihydroxy, 6 and 8 can unsubstituteds, also can be hydroxyl replacement or methoxyl group replacement; The B ring can have 1-5 hydroxyl, or other substituent group; 3 on C ring is a hydroxyl.As Quercetin, nimbecetin, acacetin, isorhamnetin, Licoflavonol. etc.
If structure aglycon isoflavone, A ring must structure be 5, the 7-dihydroxy, 6 and 8 can unsubstituteds, also can be hydroxyl replacement or methoxyl group replacement; The B ring can have 1-5, or other substituent group; 2 on C ring is a hydrogen.As sweet pea phenol, 3 '-methoxyl group sweet pea phenol, genistein etc.
This type of Chinese medicine monomer can be used to prepare the medicine that promotes the motor neuron growth, also is used to prepare the medicine that promotes that injured nerve is repaired.
Wherein said neuronal damage has probability widely, comprises the nerve injury that is caused by wound, sudden illness, disorder, motion sickness, demyelination, nerve retrograde affection.
Description of drawings:
Fig. 1: Chinese medicine monomer structural formula.
Fig. 2: the influence of the length of medicine pair cell projection.Medicine all has obvious facilitation for the growth of cell axon.Scale bar (length of the scale)=100 μ m
Fig. 3: medicine all has obvious facilitation for the growth of cell axon.Compare with PBS (phosphate buffered solution) group
*P＜0.01.
Fig. 4: different pharmaceutical is to the influence of SFI (sciatic nerve function index) value.S.O:ShamOperated (sham-operation).Compare with S.O
*P＜0.01; Compare with model group
*P＜0.05; Compare with model group
*P＜0.01.
Fig. 5: different pharmaceutical is to the influence of sufficient caudal horn.S.O：Sham?Operated。Compare with S.O
*P＜0.01; Compare with model group
*P＜0.05; Compare with model group
*P＜0.01.
Fig. 6: different pharmaceutical is to the influence at angle, vola.S.O：Sham?Operated。Compare with S.O
*P＜0.05; Compare with S.O
*P＜0.01; Compare with model group
*P＜0.05; Compare with model group
*P＜0.01.
Fig. 7: different pharmaceutical is to the influence of the length ratio of stretching one's legs.S.O：Sham?Operated。Compare with S.O
*P＜0.01; Compare with model group
*P＜0.05; Compare with model group
*P＜0.0,
Fig. 8: sciatic nerve nervous tissue form (HE hematoxylin-eosin staining 100 *).A: sham operated rats; B: operation modeling group injury region; C: operation modeling group injury region far-end; D: operation back normal saline 2 weeks of administration; E: operation back Quercetin (20mg/kg) 2 weeks of administration; F: operation back baicalin (20mg/kg) 2 weeks of administration; G: operation back normal saline 4 weeks of administration; H: operation back Quercetin (20mg/kg) 4 weeks of administration; I: operation back baicalin (20mg/kg) 4 weeks of administration. yellow arrows is represented inflammatory cell infiltration, and yellow triangle is represented vacuolar degeneration.
The specific embodiment:
1, carries out the pharmic function checking from cellular level
After medicine acted on the former foster motor neuron 24h that is commissioned to train respectively, observe the neuron axon growing state down with inverted microscope, every group 3 hole, take pictures in the some visuals field of picked at random, every hole, with IPP software measurement statistics projection length, each class value all compares with the PBS matched group, estimates the influence (Fig. 2) of medicine to the motor neuron projection length.Next, added up various Chinese medicine monomer respectively in variable concentrations lower motor neuron aixs cylinder length, found that, these five kinds of medicines all can promote the growth of neurite under finite concentration, and comparing with PBS all has significant difference (Fig. 3).
2, carry out the pharmic function checking from the whole animal level
The Wistar rat is carried out the operation of sciatic nerve and femoral nerve clamp, after the operation with medicine respectively with lumbar injection mode administration 28 days.Before detecting administration, administration 14 days, the motor capacity of administration laboratory animal in the time of 28 days and histology change, and estimate the help that medicine recovers the laboratory animal motor capacity.The laboratory animal of having added up different groups respectively is in behavioristics is detected: SFI value (Fig. 4), foot caudal horn (Fig. 5), angle, vola (Fig. 6), the length ratio (Fig. 7) etc. of stretching one's legs detects index, confirm that medicine all can promote the recovery of laboratory animal motor capacity under finite concentration, comparing with the normal saline group all has significant difference.Compared not difference (Fig. 8) on the same group the laboratory animal sciatic nerve histology by the HE colouring method again, confirmed equally that medicine can promote the impaired sciatic nerve and the functional rehabilitation of femoral nerve.
Claims (3)
1. the application of Chinese medicine monomer in the medicine that the impaired nervus motorius of preparation promotion is repaired is characterized in that described Chinese medicine monomer is meant Quercetin, baicalin, apigenin, kaempferol or genistein.
2. according to the application in the claim 1, wherein, the impaired nervus motorius reparation of described promotion can be used for treating and comprises the nerve injury disease that is caused by wound, disorder, sudden illness, motion sickness, demyelination or nerve retrograde affection.
3. according to the application in the claim 1, wherein, described medicine is meant that active component contains the medicine of any one Chinese medicine monomer in Quercetin, baicalin, apigenin, kaempferol or the genistein.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100354861A CN101690814B (en) | 2009-10-09 | 2009-10-09 | Traditional Chinese medicine monomer having function of promoting growth of motoneuron |
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| CN2009100354861A CN101690814B (en) | 2009-10-09 | 2009-10-09 | Traditional Chinese medicine monomer having function of promoting growth of motoneuron |
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| CN101690814A CN101690814A (en) | 2010-04-07 |
| CN101690814B true CN101690814B (en) | 2011-05-04 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103989669A (en) * | 2014-04-01 | 2014-08-20 | 中国药科大学 | Application of quercetin in preparation of peripheral nerve injury treatment drugs |
| CN106244548B (en) * | 2015-06-09 | 2019-07-05 | 成都中医药大学 | Purposes of the luteolin in inducing mesenchymal stem cell into the differentiation of neural cell directional |
| CN107501381A (en) * | 2017-07-19 | 2017-12-22 | 佛山市第五人民医院 | It is a kind of that there is compound for promoting motor neuron growth and its production and use |
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Non-Patent Citations (1)
| Title |
|---|
| 王新嘉等.槲皮素对糖尿病大鼠周围神经病变的保护作用.《中国药理学与毒理学杂志》.1997,233-234. * |
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Granted publication date: 20110504 Termination date: 20161009 |