CN101690752B - Quality control method of medicine composition - Google Patents
Quality control method of medicine composition Download PDFInfo
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Abstract
The invention discloses a quality control method of medicine composition, in particular to Jinji preparation quality control method. The method comprises an authentication method and/ or a content measuring method, wherein the authentication method has favourable reproducibility and no negative interference; according to the test result, the content measuring method is proved to have favourable precision degree, reproducibility and stability and can effectively control Jinji preparation product quality.
Description
Invention field
The present invention relates to a kind of method of quality control of pharmaceutical composition, particularly a kind of discrimination method and content assaying method of pharmaceutical composition of Jinji preparation for the treatment of gynecological disease.
Background technology
Prescription about JINJI JIAONANG, proterties and discriminating are on the books in the Chinese medicine ministerial standard, phonetic name: JinjiJiaonang, book page number: Z16-95 standard number: WS3-B-3070-98, prescription: the very heavy leatherleaf mahonia Radix zanthoxyli Herba Andrographitis that pulls out of cherokee rose root reticulate millettia, proterties: this product is that Capsule content is brown to tan powder and particle; Bitter.Differentiate: (1) gets the content of 6 of this product, adds 2% hydrochloric acid solution 40ml, shake well, left standstill 30 minutes, and filtered, get filter residue, wash with water to the pH value be 3～4, low temperature drying adds acetone 10ml, flooded 1 hour, constantly jolting filters, filtrate is put on the filter paper of drying, drip 1% ammonium ferric sulfate solution, aobvious grey is to the celadon spot, and spot colors does not take off after placing.(2) get the content of 10 of this product, add methyl alcohol 30ml, added hot reflux 15 minutes, filter, filtrate evaporate to dryness, residue add 2% hydrochloric acid solution 20ml makes dissolving, filter, filtrate is put in the separating funnel, with strong ammonia solution adjust pH to 10, extract 2 times with the chloroform jolting, each 15ml, combined chloroform extract, evaporate to dryness, residue adds methyl alcohol 0.3ml makes dissolving, as need testing solution.Other gets leatherleaf mahonia control medicinal material 0.4g, adds methyl alcohol 15ml, adds hot reflux 15 minutes, filters, and filtrate evaporate to dryness, residue add methyl alcohol 0.5ml and just dissolve, in contrast medicinal material solution.Get again the Berberine hydrochloride reference substance, add methyl alcohol and make the solution that every 1ml contains 0.5mg, in contrast product solution.Test according to thin-layered chromatography (appendix VI B), draw each 1 μ l of need testing solution 10 μ l, control medicinal material solution and reference substance solution, put respectively on same silica gel g thin-layer plate, take normal butyl alcohol-glacial acetic acid-water (7: 1: 2) as developping agent, launch, take out, dry, put under the ultraviolet lamp (365nm) and inspect.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, aobvious identical yellow fluorescence spot; With the corresponding position of reference substance chromatogram on, an aobvious identical yellow fluorescence spot.(3) get Herba Andrographitis control medicinal material 1g, add methyl alcohol 15ml, added hot reflux 15 minutes, filter, filtrate evaporate to dryness, residue add methyl alcohol 1ml makes dissolving, in contrast medicinal material solution.Test according to thin-layered chromatography (appendix VI B), draw need testing solution and each 5 μ l of above-mentioned control medicinal material solution under the item of (discriminating) (2), put respectively on same silica gel g thin-layer plate, take chloroform-methanol (9: 1) as developping agent, launch, take out, dry, spray is with the equivalent mixed liquor (before use mix) of 2% dinitrobenzoic acid ethanolic solution with 5% NaOH ethanolic solution.In the test sample chromatogram, with the corresponding position of control medicinal material chromatogram on, the spot of aobvious same color.Check: moisture is measured according to aquametry (appendix IX H the second method), must not cross 12.0%.Other should meet every regulation relevant under the capsule item (appendix IL).Function with cure mainly: clearing heat and detoxicating, invigorating the spleen dehumidifying, vein relaxing is invigorated blood circulation.Be used for adnexitis, endometritis, the pelvic infecton person that belongs to the syndrome of dampness-heat diffusing downward.Usage and consumption: oral, one time 4,3 times on the one.Attention: the careful usefulness of pregnant woman.Specification: every dress 0.35g.Storage: sealing.Draft in institute for drug control, Guangxi Zhuang Autonomous Region.
The present invention breaks through the method for quality control of the disclosed JINJI JIAONANG of original Chinese medicine ministerial standard, does the control method that makes new advances at discrimination method and content assaying method, and the discrimination method favorable reproducibility is negative noiseless; The content assaying method Based on testing results shows its precision, reappearance, has good stability, product quality that can more effective control Jinji preparation.
Summary of the invention
The present invention seeks to the method for quality control of open Jinji preparation, the method comprises following discriminating and/or content assaying method.
Jinji preparation be by prescription be cherokee rose root, reticulate millettia, very heavyly pull out, Chinese patent medicine preparation that leatherleaf mahonia, Radix zanthoxyli and six kinds of bulk drugs of Herba Andrographitis are made.
The discrimination method of Jinji preparation is as follows:
Method A: get 1/2 of Jinji preparation content day taking dose, porphyrize adds the 60%-95% ethanolic solution of 20-50ml, add hot reflux 0.5-4 hour, filter the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 0.5-4g, mixes thoroughly, drying is put on the silicagel column, uses first 60～90 ℃ of sherwood oil 20-50ml wash-outs, discard, use again methenyl choloride 40-80ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1-5ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether of 7: 3: 0.15-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method B: get 1/2 of Jinji preparation content day taking dose, porphyrize adds the 60%-95% ethanolic solution of 20-50ml, adds hot reflux 0.5-4 hour, filters, and the filtrate evaporate to dryness,
Residue adds water 20-50ml makes dissolving, adds the 20-50ml ethyl acetate extraction again, divides and gets acetic acid ethyl fluid, and evaporate to dryness, residue add ethyl acetate 1-5ml makes dissolving, as need testing solution.
Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution.According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate of 30: 6: 0.3-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Content assaying method is as follows:
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 5000;
The preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, the palmatin hydrochloride reference substance is an amount of, adds methyl alcohol and makes the solution that every 1ml contains reference substance 5-20 μ g;
The content of Jinji preparation is got in the preparation of need testing solution, and porphyrize is got the 1/12-1/6 of day taking dose, accurately weighed, put in the measuring bottle, add 1: 100 hydrochloric acid-methyl alcohol mixed solution 45-50ml, heating is 10-60 minute in 50-80 ℃ of water-bath, take out, let cool ultrasonic extraction 40-120 minute, put to room temperature, the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shakes up, and filters, precision measures subsequent filtrate 15ml, be added on the neutral alumina column, with 80% ethanolic solution 40-80ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Jinji preparation contains leatherleaf mahonia with Berberine hydrochloride C taking dose/every day
20H
17NO
4HCl and palmatin hydrochloride C
21H
22NO
4HCl adds up to, and must not be less than 1.2mg.
Preferred discrimination method is as follows:
Method A, get 1/2 of Jinji preparation content day taking dose, porphyrize adds 95% ethanol 20ml, add hot reflux 1 hour, and filtered the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 1g, mixes thoroughly, drying is put on the silicagel column, uses first 60～90 ℃ of sherwood oil 30ml wash-outs, discard, use again methenyl choloride 40ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, as need testing solution; Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether of 7: 3: 0.15-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method B,
Get 1/2 of Jinji preparation content day taking dose, porphyrize adds 70% ethanol 50ml, adds hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add water 30ml makes dissolving, adds the 30ml ethyl acetate extraction again, divide and get acetic acid ethyl fluid, evaporate to dryness, residue add ethyl acetate 2ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution.According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate of 30: 6: 0.3-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
The preferred content assay method is as follows:
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 5000;
The preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, the palmatin hydrochloride reference substance is an amount of, adds methyl alcohol and makes the solution that every 1ml contains 7.5 μ g;
The content of Jinji preparation is got in the preparation of need testing solution, and porphyrize is got the 1/12-1/6 of day taking dose, accurately weighed, put in the measuring bottle, add 1: 100 hydrochloric acid-methyl alcohol mixed solution 50ml, heating is 15 minutes in 55 ℃ of water-baths, take out, let cool ultrasonic extraction 45 minutes, put to room temperature, the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shakes up, and filters, precision measures subsequent filtrate 15ml, be added on the neutral alumina column, with 80% ethanolic solution 50ml wash-out, collect eluent, Recycled ethanol is to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Jinji preparation contains leatherleaf mahonia with Berberine hydrochloride C taking dose/every day
20H
17NO
4HCl and palmatin hydrochloride C
21H
22NO
4The HCl meter must not be less than 1.2mg.
The used Jinji preparation of the present invention comprises tablet, capsule, granule, pill, oral liquid, the clinical or pharmaceutically acceptable common formulations such as soft extract, wherein tablet is 2 bags of taking dose every day 18 (sugar coated tablet) or 12 (Film coated tablets) JINJI KELI taking doses every day, every bag of 8g, 12 of JINJI JIAONANG taking doses every day, 5 of spansule one time, every day 1-2 time, 12 of soft capsule taking doses every day, 12 of dripping pill taking doses every day, oral liquid 3 times on the one, each 10ml, soft extract 3 times on the one, each 10ml.Discrimination method favorable reproducibility in the method for quality control of the present invention, negative noiseless; The content assaying method Based on testing results shows its precision, reappearance, has good stability.
Description of drawings
Fig. 1: reticulate millettia medicinal material thin layer is differentiated in take methenyl choloride-ether-methyl alcohol (7: 3: 0.15) as the JINJI JIAONANG of developping agent
Fig. 2: reticulate millettia medicinal material thin-layer chromatography is differentiated in take methenyl choloride-methyl alcohol (7: 3) as the JINJI JIAONANG of developping agent
Fig. 3: the assay separating effect take 28: 72 acetonitriles-0.05mol/L sodium dihydrogen phosphate as mobile phase
Fig. 4: experimental example and embodiment are used for further specifying but are not limited to the present invention below the assay separating effect take 30: 70 acetonitriles-0.05mol/L sodium dihydrogen phosphate as mobile phase.
Experimental example 1: the choice experiment of mobile phase in the discrimination method
Reticulate millettia medicinal material thin-layer chromatography identification experiment in the JINJI JIAONANG, respectively take methenyl choloride-ether-methyl alcohol (7: 3: 0.15) and methenyl choloride-methyl alcohol (7: 3) as developping agent, unexpected discovery methenyl choloride-ether-methyl alcohol (7: 3: 0.15) is the developping agent favorable reproducibility, negative noiseless, wherein Fig. 1 is favorable reproducibility, and is negative noiseless; Though it is good that Fig. 2 is reappearance, feminine gender has interference; The thin layer plate of Fig. 1 is the silica G plate take sodium carboxymethyl cellulose as binder, 1, sample (1106066) 2, sample (1106067) 3, sample (1106068) 4, onocerin reference substance 5, negative control, developping agent: methenyl choloride-ether-methyl alcohol (7: 3: 0.15), developer: put under the ultraviolet lamp (254nm) and inspect, temperature: 31 ℃, relative humidity: 79%; The thin layer plate of Fig. 2 is the silica G plate take sodium carboxymethyl cellulose as binder, 1, sample (1106066), 2, sample (1106067), 3, sample (1106068) 4, negative control 5, onocerin reference substance, developping agent: methenyl choloride-methyl alcohol (7: 3); Developer: put under the ultraviolet lamp (254nm) and inspect temperature: 30 ℃, relative humidity: 76%.
Experimental example 2; The choice experiment of mobile phase in the assay
The content assaying method of JINJI JIAONANG: chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000.It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g, and get final product.The content of Jinji preparation is got in the preparation of need testing solution, and porphyrize is got approximately 0.5g, accurately weighed, put in the 50ml measuring bottle, add hydrochloric acid-methyl alcohol (1: 100) mixed solution 48ml, heating is 15 minutes in 55 ℃ of water-baths, takes out, and lets cool, ultrasonic extraction (power 80W, frequency 50kHz) 45 minutes, put to room temperature, add hydrochloric acid-methyl alcohol (1: 100) mixed solution and be settled to scale, shake up, filter, precision measures subsequent filtrate 15ml, is added on neutral alumina column (100～200 orders, 4g, internal diameter 1cm, dry column-packing) on, with methyl alcohol 50ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue adds methyl alcohol to be made in right amount dissolving and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.Jinji preparation contains leatherleaf mahonia with Berberine hydrochloride (C taking dose/every day
20H
17NO
4HCl) amount meter must not be less than 540 μ g.
Except the wood that there is merit, also contain the 5 flavor bulk drugs such as Radix zanthoxyli because of JINJI JIAONANG, closing with different proportion with adjustment through repetition test is mobile phase, acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid the is transferred pH2.8) separating effect that found that 27: 73 is better, and selection acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH2.8) (30: 70) is that the mobile phase separating effect is unsatisfactory, referring to Fig. 3, Fig. 4.
Experimental example 3: need testing solution extracts and the wash-out experiment in the assay
Extract experiment about need testing solution in the assay, the hydrochloric acid-methyl alcohol that uses (1: 100) extracts as solvent, extracting method be used in heat in 55 ℃ of water-baths after more ultrasonic processing, concrete operations and the results are shown in Table 1.
The selection result of table 1, extraction conditions
| Sequence number | 55 ℃ of heat time heating times (minute) | The ultrasonic processing time (minute) | Content of berberine hydrochloride (mg/g) |
| 1 | 15 | 30 | 0.315 |
| 2 | 15 | 45 | 0.365 |
| 3 | 15 | 60 | 0.367 |
As known from Table 1, with the 45 minutes extraction efficiency the bests of ultrasonic processing again after 15 minutes of heating in 55 ℃ of water-baths, therefore extraction conditions is defined as: get the preparation content, porphyrize is got approximately 0.5g, and is accurately weighed, put in the tool plug conical flask, accurate hydrochloric acid-methyl alcohol (1: 100) mixed solution 50ml that adds, weighed weight, heating is 15 minutes in 55 ℃ of water-baths, let cool, again ultrasonic processing (power 80W, frequency 50kHz) 45 minutes lets cool, weighed weight again, supply the weight of less loss with hydrochloric acid-methyl alcohol (1: 100) mixed solution, shake up, filter.
About increasing the elution step of filtrate in the assay, directly get filtrate and inject hplc determination, except the wood that there is merit, also contain 5 flavors such as Radix zanthoxyli because of JINJI JIAONANG, impurity peaks is too many, affects the judgement of main peak, removes impurity therefore increase wash-out, and row is measured again.Elution process is for " precision measures subsequent filtrate 15ml; be added on the neutral alumina column (100～200 orders, 4g, internal diameter 1cm; dry column-packing); with methyl alcohol 50ml wash-out, collect eluent, reclaims methyl alcohol to doing; residue adds methyl alcohol to be made in right amount dissolving and be transferred in the 10ml measuring bottle; add methyl alcohol to scale, shake up, and get final product.”
Experimental example 4: content assaying method neutral line relation is investigated experiment
Precision takes by weighing Berberine hydrochloride reference substance 10.21mg, put in the 50ml measuring bottle, adding methyl alcohol makes dissolving and is diluted to scale, shake up, product stock solution (concentration is 0.2042mg/ml) in contrast, precision measures above-mentioned reference substance stock solution 0.5,1,2,5,10ml, put respectively in the 25ml measuring bottle, add methyl alcohol and be diluted to scale, shake up, press respectively sample introduction 10 μ l mensuration of chromatographic condition.Take Berberine hydrochloride reference substance sample size (μ g) as horizontal ordinate (X), the peak area integrated value is ordinate (Y) drawing standard curve, and then regression equation is:
Y＝3888622X-23528.5(r＝0.9999)
The result shows: when sample size was in 0.0408～0.8168 μ g scope, Berberine hydrochloride sample size and peak area integrated value were good linear relationship.Above-mentioned test findings sees table 2 for details.
Table 2 Berberine hydrochloride reference substance linear relationship
Experimental example 5: precision test in the content assaying method
To same need testing solution, METHOD FOR CONTINUOUS DETERMINATION 5 times is calculated content.The mean value of content of berberine hydrochloride is 0.364mg/g as a result, and RSD is 0.91% (n=5).See table 3 for details.Test shows, the precision of content assaying method is better.
Table 3 Precision test result
Experimental example 6: stability test in the content assaying method
To same need testing solution, measure once at regular intervals, measure altogether 6 times, the mean value of content of berberine hydrochloride is 0.367mg/g as a result, RSD is 1.95% (n=6).Test shows, in 12 hours, measured object is stable.
Table 4 stability test result
Experimental example 7: replica test in the content assaying method
To same batch sample, 6 parts of replicate determinations the results are shown in following table.The mean value of content of berberine hydrochloride is 0.386mg/g as a result, and RSD is 1.99% (n=6).The result shows, the repeatability of this law better.
Table 5 replica test result (measurement result is twice sample introduction mean value)
The specific embodiment of the invention is as follows
The method of quality control of embodiment 1 JINJI JIAONANG
Get JINJI JIAONANG content 2g, porphyrize adds ethanol 20ml, adds hot reflux 1 hour, filter, the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 1g, mix thoroughly, drying is put silicagel column (100-200 order, 2g, internal diameter 1cm, dry column-packing) on, use first sherwood oil (60～90 ℃) 30ml wash-out, discard, use again methenyl choloride 40ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, as need testing solution.Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether-methyl alcohol (7: 3: 0.15) as developping agent, launch, take out, dry.Put under the ultraviolet lamp (254nm) and inspect.In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
The method of quality control of embodiment 2 JINJI JIAONANGs
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000.It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g, and get final product.The content under the JINJI JIAONANG content uniformity item is got in the preparation of need testing solution, and porphyrize is got approximately 0.5g, accurately weighed, put in the 50ml measuring bottle, add hydrochloric acid-methyl alcohol (1: 100) mixed solution 48ml, heating is 15 minutes in 55 ℃ of water-baths, takes out, and lets cool, ultrasonic extraction (power 80W, frequency 50kHz) 45 minutes is put to room temperature, add hydrochloric acid-methyl alcohol (1: 100) mixed solution and be settled to scale, shake up, filter, get subsequent filtrate, and get final product.Determination method is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.Every 0.35g of JINJI JIAONANG contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) amount meter must not be less than 45 μ g.
Embodiment 3: the method for quality control of JINJI PIAN
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000.
It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g, and get final product.
12 of 8 of this product Film coated tablets or sugar coated tablets are got in the preparation of need testing solution, remove dressing, porphyrize, get approximately 0.5g, accurately weighed, put in the 50ml measuring bottle, add hydrochloric acid-methyl alcohol (1: 100) mixed solution 48ml, heating is 15 minutes in 55 ℃ of water-baths, takes out, let cool ultrasonic extraction (power 80W, frequency 50kHz) 45 minutes, put to room temperature, add hydrochloric acid-methyl alcohol (1: 100) mixed solution and be settled to scale, shake up, filter, precision measures subsequent filtrate 15ml, be added on neutral alumina column (200～300 orders, 4g, internal diameter 1cm, dry column-packing) on, with methyl alcohol 50ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up, and get final product.
Determination method is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Every of this product contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) meter, Film coated tablets must not be less than 45 μ g, and sugar coated tablet must not be less than 30 μ g.
Embodiment 4: the method for quality control of JINJI KELI
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filling agent; Take acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH to 3.0) (25: 75) as mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000.
It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g, and get final product.
The content under this product content uniformity item is got in the preparation of need testing solution, and porphyrize is got approximately 2g, accurately weighed, put in the 50ml measuring bottle, add hydrochloric acid-methyl alcohol (1: 100) mixed solution 48ml, heating is 15 minutes in 55 ℃ of water-baths, takes out, and lets cool, ultrasonic extraction (power 80W, frequency 50kHz) 45 minutes, put to room temperature, add hydrochloric acid-methyl alcohol (1: 100) mixed solution and be settled to scale, shake up, filter, precision measures subsequent filtrate 15ml, is added on neutral alumina column (200～300 orders, 4g, internal diameter 1cm, dry column-packing) on, with methyl alcohol 50ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue adds methyl alcohol to be made in right amount dissolving and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.
Determination method is accurate reference substance solution, each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Every bag of this golden pheasant particle contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) meter must not be less than 0.28mg.
Embodiment 5: the method for quality control of golden pheasant spansule
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000; It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g;
The content of 1 of golden pheasant spansule is got in the preparation of need testing solution, and porphyrize is got content 1/2, accurately weighed, put in the 50ml measuring bottle, add 1: 100 hydrochloric acid-methyl alcohol mixed solution 48ml, heating is 30 minutes in 60 ℃ of water-baths, take out, let cool ultrasonic extraction 60 minutes, put to room temperature, the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shakes up, and filters, precision measures subsequent filtrate 15ml, be added on the neutral alumina column, with methyl alcohol 50ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
Golden pheasant spansule taking dose every day contains leatherleaf mahonia with Berberine hydrochloride C
20H
17NO
4The amount meter of HCl must not be less than 540 μ g.
Embodiment 6: the method for quality control of golden pheasant dripping pill
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000; It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g;
The content of 1 of golden pheasant dripping pill is got in the preparation of need testing solution, and porphyrize is accurately weighed, put in the 50ml measuring bottle, the hydrochloric acid-methyl alcohol mixed solution 48ml that adds 1: 100, heating is 10 minutes in 50 ℃ of water-baths, takes out, let cool, ultrasonic extraction 45 minutes is put to room temperature, and the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shake up, filter, precision measures subsequent filtrate 15ml, is added on the neutral alumina column, with methyl alcohol 40ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue adds methyl alcohol to be made in right amount dissolving and is transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
The golden pheasant dripping pill contains leatherleaf mahonia with Berberine hydrochloride C taking dose/every day
20H
17NO
4The amount meter of HCl must not be less than 540 μ g.
Embodiment 7: the method for quality control of oral liquid with Cherokee rose toot
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (phosphoric acid is transferred pH2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 3000.It is an amount of that the preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, adds methyl alcohol and make the solution that every 1ml contains 15 μ g, and get final product.
Oral liquid with Cherokee rose toot 5ml is got in the preparation of need testing solution, and is accurately weighed, puts in the 50ml measuring bottle, add hydrochloric acid-methyl alcohol (1: 100) mixed solution 48ml, heating is 15 minutes in 55 ℃ of water-baths, takes out, and lets cool, ultrasonic extraction (power 80W, frequency 50kHz) 45 minutes, put to room temperature, add hydrochloric acid-methyl alcohol (1: 100) mixed solution and be settled to scale, shake up, filter, precision measures subsequent filtrate 15ml, is added on neutral alumina column (100～200 orders, 4g, internal diameter 1cm, dry column-packing) on, with methyl alcohol 50ml wash-out, collect eluent, reclaim methyl alcohol to doing, residue adds methyl alcohol to be made in right amount dissolving and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.
Oral liquid with Cherokee rose toot taking dose every day contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) amount meter must not be less than 540 μ g.
Embodiment 8: the method for quality control of golden pheasant dripping pill
Get 6 of golden pheasant dripping pill contents, porphyrize adds the ethanolic solution of 60% 30ml, add hot reflux 2 hours, and filtered the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 2g, mixes thoroughly, drying is put on the silicagel column, uses first 60～90 ℃ of sherwood oil 30ml wash-outs, discard, use again methenyl choloride 50ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 3ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether of 7: 3: 0.15-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
Embodiment 9: the method for quality control of golden pheasant soft extract processed
Get golden pheasant soft extract 15ml processed, add 95% ethanol 20ml, added hot reflux 1 hour, filter, the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 1g, mixes thoroughly, drying is put silicagel column (100-200 order, 2g, internal diameter 1cm, dry column-packing) on, use first sherwood oil (60～90 ℃) 30ml wash-out, discard, use again methenyl choloride 40ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, as need testing solution; Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether-methyl alcohol (7: 3: 0.15) as developping agent, launch, take out, dry; Put under the ultraviolet lamp (254nm) and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
The discrimination method of embodiment 10 JINJI JIAONANGs
Get JINJI JIAONANG content 2g, porphyrize adds 70% ethanol 50ml, adds hot reflux 30 minutes, filter, and the filtrate evaporate to dryness,
Residue adds water 30ml makes dissolving, adds the 30ml ethyl acetate extraction again, divides and gets acetic acid ethyl fluid, and evaporate to dryness, residue add ethyl acetate 2ml makes dissolving, as need testing solution.
Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution.According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate of 30: 6: 0.3-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
Above-mentioned used JINJI JIAONANG usage and consumption: oral, one time 4,3 times on the one.Specification: every dress 0.35g.
Embodiment 11: the content assaying method of JINJI JIAONANG
[assay] photograph high performance liquid chromatography (" an appendix VI of Chinese Pharmacopoeia 2005 version D) measure.
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (with phosphorus acid for adjusting pH value 2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by palmatin hydrochloride peak and Berberine hydrochloride peak should be not less than 5000.
The Berberine hydrochloride reference substance is got in the preparation of reference substance solution, the palmatin hydrochloride reference substance is an amount of, and is accurately weighed, adds methyl alcohol and makes the solution that every 1ml contains 7.5 μ g, and get final product.
The content under this product content uniformity item is got in the preparation of need testing solution, and porphyrize is got approximately 0.5g, accurately weighed, put in the tool plug conical flask accurate hydrochloric acid-methyl alcohol (1: 100) mixed solution 50ml that adds, close plug, weighed weight, heating is 15 minutes in 55 ℃ of water-baths, ultrasonic processing (power 400W, frequency 40kHz) 45 minutes lets cool, weighed weight is again supplied the weight of less loss with hydrochloric acid-methyl alcohol (1: 100) mixed solution, shake up, filter, precision measures subsequent filtrate 15ml, by neutral alumina column (100～200 orders, 5g, internal diameter 1.0cm, dry column-packing) on, with 80% ethanol 50ml wash-out, collects eluent, Recycled ethanol is to doing, residue dissolves with methyl alcohol, and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Every of this product contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) and palmatin hydrochloride (C
21H
22NO
4HCl) meter must not be less than 0.10mg.
Above-mentioned used JINJI JIAONANG usage and consumption: oral, one time 4,3 times on the one.Specification: every dress 0.35g.
Embodiment 12: the JINJI KELI method of quality control
Discrimination method: get JINJI KELI 16g, porphyrize adds 70% ethanol 50ml, adds hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add water 30ml makes dissolving, adds ethyl acetate 30ml jolting and extracts, divide and get acetic acid ethyl fluid, evaporate to dryness, residue add ethyl acetate 2ml makes dissolving, as need testing solution.Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate-methyl alcohol (30: 6: 0.3) as developping agent, launch, take out, dry, put under the ultraviolet lamp (254nm) and inspect.In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
[assay] measured according to high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 D).
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Take acetonitrile-0.05mol/L sodium dihydrogen phosphate (with phosphorus acid for adjusting pH value 2.8) (27: 73) as mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by palmatin hydrochloride and Berberine hydrochloride peak should be not less than 5000.
The Berberine hydrochloride reference substance is got in the preparation of reference substance solution, the palmatin hydrochloride reference substance is an amount of, and is accurately weighed, adds methyl alcohol and makes the solution that every 1ml contains 7.5 μ g, and get final product.
The content under this product content uniformity item is got in the preparation of need testing solution, and porphyrize is got approximately 2g, accurately weighed, put in the tool plug conical flask accurate hydrochloric acid-methyl alcohol (1: 100) mixed solution 50ml that adds, close plug, weighed weight, heating is 15 minutes in 55 ℃ of water-baths, ultrasonic processing (power 400W, frequency 40kHz) 45 minutes lets cool, weighed weight is again supplied the weight of less loss with hydrochloric acid-methyl alcohol (1: 100) mixed solution, shake up, filter, precision measures subsequent filtrate 15ml, is added on neutral alumina column (100 ~ 200 orders, 5g, internal diameter 1.0cm, dry column-packing) on, with 80% ethanol 50ml wash-out, collects eluent, Recycled ethanol is to doing, residue dissolves with methyl alcohol, and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Every bag of JINJI KELI contains leatherleaf mahonia in Berberine hydrochloride (C20H17NO4HCl) and palmatin hydrochloride (C21H22NO4HCl), must not be less than 0.70mg.
The every packed 8g of above-mentioned used JINJI KELI takes after mixing it with hot water, one time 1 bag, 2 times on the one.
Embodiment 13 golden pheasant tablet method of quality control
Get 6 of 9 of this product sugar coated tablets or Film coated tablets, remove dressing, porphyrize, add 70% ethanol 50ml, add hot reflux 30 minutes, and filtered the filtrate evaporate to dryness, residue adds water 30ml makes dissolving, extract with ethyl acetate 30ml jolting, divide and get acetic acid ethyl fluid, evaporate to dryness, residue adds ethyl acetate 2ml makes dissolving, as need testing solution.Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate-methyl alcohol (30: 6: 0.3) as developping agent, launch, take out, dry, put under the ultraviolet lamp (254nm) and inspect.In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color.
[assay] photograph high performance liquid chromatography (" an appendix VI of Chinese Pharmacopoeia 2005 version D) measure.
Chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; Acetonitrile-0.05mol/L sodium dihydrogen phosphate (with phosphorus acid for adjusting pH value 2.8) (27: 73) is mobile phase; The detection wavelength is 265nm.Number of theoretical plate calculates by palmatin hydrochloride peak and Berberine hydrochloride peak should be not less than 5000.
The Berberine hydrochloride reference substance is got in the preparation of reference substance solution, the palmatin hydrochloride reference substance is an amount of, and is accurately weighed, adds methyl alcohol and makes the solution that every 1ml contains 7.5 μ g, and get final product.
10 of this product are got in the preparation of need testing solution, remove dressing, porphyrize, get approximately 0.5g, accurately weighed, put in the tool plug conical flask, accurate hydrochloric acid-methyl alcohol (1: 100) mixed solution 50ml, close plug, the weighed weight of adding, heating is 15 minutes in 55 ℃ of water-baths, takes out ultrasonic processing (power 400W, frequency 40kHz) 45 minutes, lets cool, more weighed weight, supply the weight of less loss with hydrochloric acid-methyl alcohol (1: 100) mixed solution, shake up, filter, precision measures subsequent filtrate 15ml, is added on neutral alumina column (100～200 orders, 5g, internal diameter 1.0cm, dry column-packing) on, with 80% ethanol 50ml wash-out, collects eluent, Recycled ethanol is to doing, residue dissolves with ethanol, and is transferred in the 10ml measuring bottle, adds methyl alcohol to scale, shake up, and get final product.
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
Every of this product contains leatherleaf mahonia with Berberine hydrochloride (C
20H
17NO
4HCl) and palmatin hydrochloride (C
21H
22NO
4HCl) total amount meter, sugar coated tablet must not be less than 70 μ g, and Film coated tablets must not be less than 105 μ g.
Every heavy 0.47g of above-mentioned golden pheasant Film coated tablets; The heavy 0.27g of sugar coated tablet substrate.4 of Film coated tablets one time, 6 of sugar coated tablets one time; 3 times on the one.
Claims (3)
1. the quality determining method of a Jinji preparation, this Jinji preparation by cherokee rose root, reticulate millettia, very heavyly pull out, leatherleaf mahonia, Radix zanthoxyli and six kinds of bulk drugs of Herba Andrographitis make, and it is characterized in that this quality determining method comprises following discriminating and content assaying method:
Method A: get 1/2 of Jinji preparation content day taking dose, porphyrize adds the 60%-95% ethanolic solution of 20-50ml, add hot reflux 0.5-4 hour, filter the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 0.5-4g, mixes thoroughly, drying is put on the silicagel column, uses first 60～90 ℃ of sherwood oil 20-50ml wash-outs, discard, use again methenyl choloride 40-80ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1-5ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether of 7: 3: 0.15-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method B: get 1/2 of Jinji preparation content day taking dose, porphyrize adds the 60%-95% ethanolic solution of 20-50ml, adds hot reflux 0.5-4 hour, filters, and the filtrate evaporate to dryness,
Residue adds water 20-50ml makes dissolving, adds the 20-50ml ethyl acetate extraction again, divides and gets acetic acid ethyl fluid, and evaporate to dryness, residue add ethyl acetate 1-5ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate of 30: 6: 0.3-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method C: chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 5000;
The preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, the palmatin hydrochloride reference substance is an amount of, adds methyl alcohol and makes the solution that every 1ml contains reference substance 5-20 μ g;
The content of Jinji preparation is got in the preparation of need testing solution, and porphyrize is got the 1/12-1/6 of day taking dose, accurately weighed, put in the measuring bottle, add 1: 100 hydrochloric acid-methyl alcohol mixed solution 45-50ml, heating is 10-60 minute in 50-80 ℃ of water-bath, take out, let cool ultrasonic extraction 40-120 minute, put to room temperature, the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shakes up, and filters, precision measures subsequent filtrate 15ml, be added on the neutral alumina column, with 80% ethanolic solution 50ml wash-out, collect eluent, Recycled ethanol is to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product;
Jinji preparation contains leatherleaf mahonia with Berberine hydrochloride C taking dose/every day
20H
17NO
4HCl and palmatin hydrochloride C
21H
22NO
4The HCl meter must not be less than 1.2mg.
2. quality determining method as claimed in claim 1 is characterized in that this quality determining method comprises following discriminating and content assaying method:
Method A, get 1/2 of Jinji preparation content day taking dose, porphyrize adds 95% ethanol 20ml, add hot reflux 1 hour, and filtered the filtrate evaporate to dryness, residue makes dissolving in right amount with methyl alcohol, adds silica gel 1g, mixes thoroughly, drying is put on the silicagel column, uses first 60～90 ℃ of sherwood oil 30ml wash-outs, discard, use again methenyl choloride 40ml wash-out, collect eluent, evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, as need testing solution; Other gets the onocerin reference substance and adds in right amount methyl alcohol and make the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ether of 7: 3: 0.15-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method B, get 1/2 of Jinji preparation content day taking dose, porphyrize adds 70% ethanol 50ml, adds hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add water 30ml makes dissolving, adds the 30ml ethyl acetate extraction again, divide and get acetic acid ethyl fluid, evaporate to dryness, residue add ethyl acetate 2ml makes dissolving, as need testing solution;
Other gets the onocerin reference substance, adds methyl alcohol and makes the solution that every 1ml contains 1mg, in contrast product solution; According to the thin-layered chromatography test, draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as binder, take methenyl choloride-ethyl acetate of 30: 6: 0.3-methyl alcohol as developping agent, launch, take out, dry; Put under the 254nm ultraviolet lamp and inspect; In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, the spot of aobvious same color;
Method C chromatographic condition and system suitability are take octadecylsilane chemically bonded silica as filling agent; With phosphoric acid transfer pH2.8 27: 73 acetonitrile-the 0.05mol/L sodium dihydrogen phosphate is mobile phase; The detection wavelength is 265nm; Number of theoretical plate calculates by the Berberine hydrochloride peak should be not less than 5000;
The preparation precision of reference substance solution takes by weighing the Berberine hydrochloride reference substance, the palmatin hydrochloride reference substance is an amount of, adds methyl alcohol and makes the solution that every 1ml contains 7.5 μ g;
The content of Jinji preparation is got in the preparation of need testing solution, and porphyrize is got the 1/12-1/6 of day taking dose, accurately weighed, put in the measuring bottle, add 1: 100 hydrochloric acid-methyl alcohol mixed solution 50ml, heating is 15 minutes in 55 ℃ of water-baths, take out, let cool ultrasonic extraction 45 minutes, put to room temperature, the hydrochloric acid-methyl alcohol mixed solution that adds 1: 100 is settled to the 50ml scale, shakes up, and filters, precision measures subsequent filtrate 15ml, be added on the neutral alumina column, with 80% ethanolic solution 50ml wash-out, collect eluent, Recycled ethanol is to doing, residue add methyl alcohol make in right amount the dissolving and be transferred in the 10ml measuring bottle, add methyl alcohol to scale, shake up;
Determination method is accurate reference substance solution and each 20 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product;
Jinji preparation contains leatherleaf mahonia with Berberine hydrochloride C taking dose/every day
20H
17NO
4HCl and palmatin hydrochloride C
21H
22NO
4The HCl meter must not be less than 1.2mg.
3. quality determining method as claimed in claim 1 or 2 is characterized in that Jinji preparation refers to tablet in this quality determining method, capsule, granule, pill, oral liquid, soft extract.
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| CN102721782B (en) * | 2012-07-02 | 2014-03-26 | 涂瑶生 | Method for detecting quality of philippine flemingia root formula granules |
| CN103604901B (en) * | 2013-11-18 | 2015-05-20 | 广州白云山和记黄埔中药有限公司 | Thin-layer identification method of compound andrographis tablet |
| CN103760263A (en) * | 2014-01-14 | 2014-04-30 | 江西金顶药业有限公司 | Quality detection method of vine flemingia |
| CN110412198B (en) * | 2018-04-28 | 2021-08-31 | 陕西步长制药有限公司 | Detection method of traditional Chinese medicine composition for treating qi deficiency and blood stasis syndrome |
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| CN1733105A (en) * | 2005-08-18 | 2006-02-15 | 贵阳云岩西创药物科技开发有限公司 | Preparation for treating gynecological disease, its preparation process and quality control method |
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Address after: No. 68, Anshan West Road, Babu District, Hezhou City, Guangxi Zhuang Autonomous Region Patentee after: Jinji Pharmaceutical Co.,Ltd. Address before: 542800 No. 68 West Anshan Road, the Guangxi Zhuang Autonomous Region, Hezhou Patentee before: Guangxi LingFeng Pharmaceutical Co.,Ltd. |
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Effective date of registration: 20200108 Address after: 535416, Lingshan Qinzhou land Industrial Park, the Guangxi Zhuang Autonomous Region Patentee after: GUANGXI GEHONGTANG PHARMACEUTICAL Co.,Ltd. Address before: No. 68, Anshan West Road, Babu District, Hezhou City, Guangxi Zhuang Autonomous Region Patentee before: Jinji Pharmaceutical Co.,Ltd. |
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Effective date of registration: 20220127 Address after: 535021 Real Madrid Industrial Park, Qinzhou City, Guangxi Zhuang Autonomous Region Patentee after: GUANGXI BANGQI PHARMACEUTICAL GROUP Co.,Ltd. Address before: 535416 Lingshan Luwu Industrial Park, Qinzhou City, Guangxi Zhuang Autonomous Region Patentee before: GUANGXI GEHONGTANG PHARMACEUTICAL Co.,Ltd. |