CN1016857B - Method of preparation of inoculum sections for plant tissue culture - Google Patents
Method of preparation of inoculum sections for plant tissue cultureInfo
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- CN1016857B CN1016857B CN 88108381 CN88108381A CN1016857B CN 1016857 B CN1016857 B CN 1016857B CN 88108381 CN88108381 CN 88108381 CN 88108381 A CN88108381 A CN 88108381A CN 1016857 B CN1016857 B CN 1016857B
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Abstract
A method of preparing inoculum sections for plant tissue culture using an apparatus comprising rotating, reciprocating or vibrating cutting elements. The method can be applied to any plant and plant parts to prepare a large amount of cut plant sections by an industrial process.
Description
The present invention relates to prepare in a large number the method for cultivating inoculum sections for plant tissue.
The general inoculation section of adopting manual method preparation to cultivate plant tissue each body of required plant or tissue for example, cuts into plant or with perforator plant is cut into disk with scalpel.These methods help operating personnel and choose the part that is suitable for cultivating most on vegetable material, and make operating personnel plant can be cut into the shape that is suitable for cultivating most.The level but this operation requires much skill, and time-consuming.Therefore, though this common method can be used for the experiment work in the laboratory, be difficult in the commercial plant propagation industry and cultivate section in order to preparation.
The present invention is surprised to find that: according to the kind of vegetable material, the choose reasonable dicing method with the section of growth of can achieving success of the method for machinery section, and does not have any problem or difficulty.
Therefore, the object of the present invention is to provide a kind of method of utilizing the mechanical device preparation in quantity to cultivate inoculum sections for plant tissue.
The concrete steps of the inventive method are:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is sent into slicing device with the preparation plant section, slicing device has disk 3 or 53, disk has an above elongated opening 4 or 54, the a certain tangent line of opening and disk 3 or 53 is provided with at angle, with drive unit 2 or 52 rotary disks, this device also has more than one the blade 5 or 54 on the fixing disk 3 or 53, should make blade 6 in the section 5 or 55 be positioned at opening 4 or 54 above, and between the plane, surperficial place of blade, leave the gap at blade 6 and disk.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material with the preparation plant section, slicing device has some guide plates 12 parallel to each other and is contained between the guide plate 12 and parallel with it some cutting knifes 17, rotatable, the reciprocating or vibration of cutting knife 17, thus when vegetable material between guide plate 12 by the time its cutting is in blocks.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material with the preparation plant section, cutting knife has fixing cutter hub 20 and the movable cutter hub 21 of paying paid, the fixing cutter hub 20 of paying has some blades that form by fixing two surfaces of paying cutter hub to intersect, movable pay that two surfaces that cutter hub 21 has some movable pair of cutter hubs are intersected and the blade that forms, wherein movably pay an end face 5 of cutter hub 21 and do sliding-contact with a fixing surface of paying cutter hub 20, therefore, movably paying cutter hub 21 rotates with respect to the fixing cutter hub 20 of paying, reciprocating or vibration and will to press to the vegetable material cutting of cutting knife in blocks.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material, slicing device has a pair of roller parallel to each other 25 at least, gapped between the roller 25, in order to transmit vegetable material, then be provided with some cutting knifes 27 in the downstream of roller 25, cutting knife rotates or reciprocating around an axle that is parallel to roller 25 axis, and the material cutting of the plant that will be transmitted by roller is in blocks.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material with the preparation plant section, slicing device has a pair of parallel to each other and leaving the roller 32 of spacing and be arranged on some cutting knifes 33 between the roller 32 at least, each cutting knife is fixed on the axle that is parallel to roller axis, and this axle rotates with drive unit.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material, slicing device has two rollers 36 at least, one of them roller has some grooves 37 on its periphery surface, another roller has some grooves on its periphery surface on the height different with the groove on the relative roller, be equipped with a cutting knife 38 that stretches out from bottom land in each groove.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation is through the vegetable material of sterilization or sterilization;
Vegetable material is pressed to the cutting knife of slicing device or the cutting knife of slicing device is pressed to vegetable material, slicing device has some parallel to each other or be latticed cutting knife 42, the blade 46 of each cutting knife 42 is towards same direction, and each cutting knife 49 also can be provided with radially, and the blade of each cutting knife is towards radiation center.
The present invention also provides a kind of preparation in quantity to cultivate the method for inoculum sections for plant tissue, it is characterized in that:
Preparation comprises the liquid medium through the vegetable material of sterilization or sterilization; With rotation, cutting knife reciprocating or vibration vegetable material is cut into plant section.
Fig. 1 is the vertical cross section of this method equipment therefor embodiment;
Fig. 2 is a horizontal sectional drawing embodiment illustrated in fig. 1;
Fig. 3 is an A-A profile among Fig. 2;
Fig. 4 is the profile of this method equipment therefor embodiment;
Fig. 5 is a plan view embodiment illustrated in fig. 4;
Fig. 6 is a B-B profile among Fig. 5;
Fig. 7 is the sketch of this method equipment therefor embodiment;
Fig. 8 is the sketch of another embodiment of this method equipment therefor;
Fig. 9 is the sketch of the another embodiment of this method equipment therefor;
Figure 10 is a C-C profile among Fig. 9;
Figure 11 is the sketch of the another embodiment of this method equipment therefor;
Figure 12 is a D-D profile among Figure 11;
Figure 13 is the sketch of the another embodiment of this method equipment therefor;
Figure 14 is the sketch of the another embodiment of this method equipment therefor;
Figure 15 is the sketch of the another embodiment of this method equipment therefor;
Figure 16 is the sketch of the another embodiment of this method equipment therefor;
Figure 17 is the sketch of the another embodiment of this method equipment therefor, and wherein: the section of vegetable material is carried out in the working fluid medium;
Figure 18 is the sketch of another embodiment of this method equipment therefor, and wherein: the section of vegetable material is carried out in liquid medium;
Figure 19 is the sketch of another embodiment of this method equipment therefor, and wherein, the section of vegetable material is carried out in liquid medium;
Figure 20 is the enlarged drawing of each cutting knife of device shown in Figure 19;
Figure 21 is the bottom view of each cutting knife of device shown in Figure 19;
Figure 22 is the perspective view of device cutting knife shown in Figure 20;
Figure 23 is a curve map, and the result who adopts device shown in Figure 1 that cylindrical potato is cut into slices is shown, show rotating speed, gained slice thickness and and the irrelevance three between relation;
Figure 24 is the curve map similar to Figure 23, and different is: show the weight that is added on the potato, the thickness of gained section and the relation between the irrelevance three thereof.
The inoculation that can prepare a large amount of various different plants by the present invention is cut into slices, just: adopt this method to comprise parts (hereinafter referred to as " vegetable material ") such as napiform root, stem tuber, stalk stem to various different pieces or the organ of plant, cut into slices.Vegetable material can be taken from the plant that not be infected with by bacterium or mould one class phytopathogen, as: the plant that desirable comfortable aseptic condition is cultivated down, also can adopt the commonsense method of any unlikely destruction plant germination ability that plant is sterilized, for example: available bactericide, fungicide or clarificant are handled vegetable material.
By a kind of preferred embodiment of the present invention, can adopt Fig. 1-6 shown device to prepare plant section.As shown in Figure 1, this device has casing 1 and by the disk 3 of drive unit 2 transmissions.The detailed disk 3 that shows of Fig. 2 has one or more opening 4, and opening 4 can become a predetermined angle to be provided with a certain tangent line of disk 3.This angle is similar to a right angle in the present embodiment, that is: opening is roughly along the radial direction setting.And for example shown in Figure 2, blade 5 is fixed on the disk 3, and make blade be positioned at opening above, thereby force the plant section that downcuts from vegetable material with blade 5 to be dropped in the casing 1 by opening 4.Press close at blade 6 and disk 3 between the plane, surperficial place 7 of blade 5 and leave the gap.This gap can be regulated by the pad (not shown) of selecting different blades 5 thickness for use or insert desired thickness between blade 5 and disk 3, with the thickness of controlling plant section.
Vegetable material, particularly napiform root or stem tuber preferably adopt " sliding cutting " and do not adopt " crush-cutting ", to alleviate the destruction to plant tissue as far as possible.Here, the meaning of " sliding cutting " is: blade is in the anterior slip of the cutting of vegetable material when carrying out the vegetable material section.For this reason, in embodiment illustrated in fig. 2, blade 5 and opening 4 are curved.When blade 6 rusts, perhaps, when need readjust gap between blade and the plane, disk 3 surperficial place, need to change blade 5.For this reason, available screw 8 is fixed on blade 5 on the disk 3, and can unload. and available motor one class drive unit is by an axle rotary disk 3.Disk 3 is fixed on the upper end of axle, but can unload so that take out the section that accumulates in the casing 1.
Device shown in Figure 1 preferably also is provided with and covers 10, covers to be provided with inlet 9.
When device work shown in Figure 1, the rotating speed of the disk 3 that drives with electromotor one class drive unit 2 can be regulated between 100-400rpm, and the gap between blade 6 and the plane, disk 3 surperficial place 7 can be regulated between 1-4mm.Concrete requirement can be selected according to the thickness of required section and the factors such as hardness of the material that need cut plant.
Device shown in Figure 1 is best suited for the plant section that lily one class napiform root or potato one class stem tuber are taken from preparation.
Vegetable material is filled in inlet 9, and the blade 5 that is driven by the disk 3 that rotates promptly carries out the section of vegetable material.When the soft vegetable material of lily napiform root one class was cut into slices, the weight of vegetable material itself just was enough to be pressed to blade, so the operator only needs vegetable material is placed on the disk and gets final product.But to potato stem tuber one class hard vegetable material when cutting into slices, except that the weight of vegetable material own, still need vegetable material exerted pressure in addition, for this reason, need apply about 50-500g/cm to vegetable material
2Pressure.This power can directly or by pressing plate be added in a weight on the vegetable material, and also available spring is added in power on the pressing plate, and also available hand is added in power on the pressing plate.
In operating process, vegetable material is cut into some, section is dropped into casing 1 by opening 4.Section accumulates in the casing 1, unloads lower cover 10 and disk 3, can take out section.
Fig. 4-6 illustrates a kind of flexible program embodiment illustrated in fig. 1.In this variation device, on disk 53, be provided with four openings 54, therefore, four blades 55 are fixed on the disk 53 with screw 57.In this variation device, blade 55 has straight blade.In this case, for ease of vegetable material is carried out " sliding cutting ", blade 55 and opening 54 become the angle setting of on-right angle in the lump with respect to a certain tangent line of disk 53, as shown in Figure 5.
Fig. 6 is the B-B profile of disk 53 among Fig. 5.In the embodiment shown in fig. 6, blade 55 is fixed on the disk 53 by pad 56.The effect of pad 56 was described in detail in explanation embodiment illustrated in fig. 1, and in variation device shown in Figure 4, disk 53 and the blade 55 that is contained in above it are positioned at casing 51.On casing 51, be provided with window 59,, wherein accumulate the quantity of section with judgement as figure in order to observe the inner case of casing 51.On casing 51, also be provided with the air inlet pipe 62 of band air cleaner 63.Therefore, under situation about whole device having been carried out disinfection before the operation beginning, in operating process, feed filtrated air by air cleaner 63 and air inlet pipe 62 and just can make maintenance germ-free condition in the casing 51.What deserves to be mentioned is: whole device can be made with metal, therefore can use the method for heating, and for example steam heated method carries out disinfection.
Can adopt the electromotor one class drive unit 52 that is located at casing 51 outsides driving force to be passed to disk 53 and make its rotation by flexible drive shaft 64.
On device shown in Figure 4, also be provided with inlet 60 and outlet 61, respectively in order to send into vegetable material and to take out plant section.In this variation device, inlet 60 is obliquely installed, and sends into vegetable material so that avoid flexible drive shaft 64.
The operating position of device shown in Figure 4 is identical with the device shown in Figure 1 that above detailed description is crossed.
In addition, device shown in Figure 4 also is applicable to the section of carrying out vegetable material in the culture medium one class I liquid I medium of water, buffer solution, plant tissue very much.In this case, liquid medium and vegetable material section 60 are sent into from entering the mouth, the liquid medium that will contain plant section is emitted from exporting 61.
Fig. 7 illustrates a kind of embodiment of this method equipment therefor briefly, and this device has casing 11, plurality of fixed at the guide plate on the casing 11 12, between guide plate 12 and the movable cutting knife 14 that be arranged in parallel with it, and pressing plate 15.Cutting knife 14 is generally the disk that has one or more blades on periphery, makes its rotation, reciprocating or vibration by transmission device, so that vegetable material is cut into slices.
In the suggested design of present embodiment, as shown in Figure 7, cutting knife on periphery, have blade 19 ' disk 14.Disc cutter 14 is fixed in the rotating shaft 13, makes rotating shaft 13 pass the hole of each guide plate 12, and makes its rotation with drive unit 18.On casing 11, be provided with inlet 16 and outlet 17, respectively in order to send into vegetable material and to take out section.
In the course of work of device, 16 send into the vegetable material section by entering the mouth, push vegetable material to guide plate 12 by pressing plate 15, both pushed pressing plate 15 to guide plate 12 nearby from 16 the rear of entering the mouth as shown in Figure 7 with manual or mechanically operated method, force the vegetable material section to enter between the two adjacent guide plates 12, thereby cut into slices by 14 pairs of vegetable material sections of the rotary disk cutting knife between two guide plates 12.
Fig. 8 illustrates the another embodiment of this method equipment therefor briefly, and this device has casing 19, cutting knife 20 and 21 and pressing plate 22.On casing 19, be provided with inlet 24 and outlet 23, respectively in order to vegetable material is sent into casing 19 and plant section is taken out from casing 19.Cutting knife is made of two cutter hubs, promptly is made of the fixed cutter body 20 and the movable cutter hub 21 that are fixed on the casing 19.Cutter hub 20 and 21 respectively has some two faces by this cutter hub to intersect each other and the blade that forms constitutes a surface of movable cutter hub 21 blades and does sliding-contact each other with a surface that constitutes fixed cutter body 20 blades.Cutter hub 20 and 21 is easy to for example make with the metallic plate that has some openings or hole, for example can make with the metallic plate that has some elongated openings or circular hole.In this case, blade can be intersected each other and formed by a surface of cutter hub 20 or 21 and a surface in opening or the hole.Cutter hub 20 and 21 yet can be made of a tier pole spare, and rod member has two corner angle at least and is parallel to each other.
The working condition of device shown in Figure 8, it is rotatable, reciprocating or vibrate to remove movable cutter hub 21, so that outside vegetable material cut into slices, all the other were identical with device shown in Figure 7.
Fig. 9 and Figure 10 illustrate the another embodiment of apparatus of the present invention, and this device has casing 24, and gapped roller 25 in the middle of at least one pair of is located at the cutting knife 27 and the pressing plate 28 in roller 25 downstreams.In the present embodiment, the motion by this pair of rollers 25 makes vegetable material press to cutting knife.Cutting knife 27 is generally the disk that has blade on periphery, and cutting knife is perpendicular to the axis of roller 25, can rotate or can be reciprocating around the axle that is parallel to roller 25 axis.
In this preferred embodiment, cutting knife 27 is made of some disks parallel to each other that have blade on periphery, and these disks are fixed on the axle 26 that is parallel to roller 25 axis, can pass through electromotor one these disks of class drive unit (not shown) transmission.In preferred embodiment shown in Figure 10, on the periphery surface of roller 25, be provided with groove 31, disc cutter 27 is embedded in the groove 31, but is not in contact with it.Like this, cutting knife 27 unlikely roller that touches when vegetable material is cut into slices.Figure 10 is the C-C profile of Fig. 9 middle roller 25 and cutting knife 27.
The working condition of device shown in Figure 9, except that forcing the vegetable material that is pushed by pressing plate to be pressed to the cutting knife 27, all the other are identical with the device shown in Figure 7 that has described in detail.For this reason, two rollers 25 rotate with opposite direction by electromotor one class drive unit (not shown) each other, so that plant is pressed to cutting knife 27.Cutting knife 27 available motor one class drive unit (not shown) is rotated to either direction.
As a kind of flexible program embodiment illustrated in fig. 9, available along the reciprocating cutting knife replacement rotary disk cutting knife of vegetable material moving direction.Cutting knife also can be relative by blade, is parallel to two plate shape cutter hubs formations by the plane of two roller axis.In this case, a plate shape cutter hub is fixed on the casing, and another can be reciprocating, when two blades separately form an opening, sends vegetable material to opening by the rotation of roller, and, vegetable material is cut into slices each other during closed contact at two blades.
Figure 11 and 12 illustrates another embodiment of this method equipment therefor briefly, the cutter 33 of device shown in Figure 11 is made of the some rotary disks that have blade on periphery, as shown in figure 12, the remainder of this device is then identical with device shown in Figure 9 between pair of parallel roller 32.Be provided with groove 34 on the periphery surface of roller 32, cutting knife 33 embeds in the groove 34, but is not in contact with it.
In device shown in Figure 13, on roller 32, be provided with hook-like part 35, these hook-like parts also can be located in Fig. 9 and the device shown in Figure 11.This structure can prevent that vegetable material from entering the gap between tank wall and the roll surface and leaking and cut, and can make it turn back to the upstream section of roller.
Figure 14 illustrates the another embodiment of this method equipment therefor briefly.This device has two rollers 36 at least, and the periphery surface of a roller is provided with some grooves 37 therein, on the periphery surface on another roller, with relative roller on the Different Plane of groove on be provided with some grooves.In each groove, be provided with the disc cutter 38 that stretches out from bottom land.
Figure 15 illustrates the another embodiment of this method equipment therefor briefly, and this device has casing 41, and casing 41 has inlet 44, outlet 45, cutting knife 42 and pressing plate 43.Constitute at the plate of this device inner cutter 42 by some band blades.These cutting knifes 42 be arranged in parallel, towards same direction.It is latticed that cutting knife 42 yet can be, towards same direction.In the course of work of device, vegetable material 44 is sent into from entering the mouth, in casing 41, accumulate a considerable amount of vegetable material after, push the manual or mechanically operated method of pressing plate 43 usefulness to cutting knife 42 from 44 the rear of entering the mouth, vegetable material is delivered on the cutting knife 42, be pressed on the blade 46, and cutting is in blocks.Section can be taken out from exporting 45.But in such an embodiment, the gap between the adjacent cutting knife 42 is often stopped up by plant section.
Can adopt flexible program shown in Figure 16 for addressing this problem, this device is similar to device shown in Figure 15, but cutting knife 49 is done radial arrangement.In this embodiment, pressing plate 47 has the convexity surface, matches with the formed recessed circle of the blade of each cutting knife 49.Casing 48 is preferably cylindrical.
In Figure 15 and 16, with pressing plate 43 or 47 vegetable material is passed on cutting knife 46 or 49, but cutting knife is moved to plate 43 or 47, to push vegetable material and to cut into slices.In this case, plate 43 or 47 keeps motionless.
By the detailed description of Fig. 7-16 shown device being done relevant vegetable material section, though do not adopt liquid medium, these devices also all can be in order to cut into slices to vegetable material in liquid medium.For this reason, as illustrate shown in Figure 17ly, can insert in the flow circuits of the medium contain vegetable material by device.
Though Fig. 7-16 shown device all is horizontally disposed with, and also all can do vertical setting.
Fig. 7-16 shown device is best suited for the section that preparation has the plant of other flower bud, as common rose, sweetheart rose, carnation, chrysanthemum etc.It should be noted that: for these plants, when the preparation section, even the sliced section beyond the other flower bud is damaged, as long as guarantee that section has complete other flower bud and both can.Owing to adopt said apparatus can produce section, adopt these devices just can make a large amount of industrial production of cultivating to above-mentioned plant with section with complete other flower bud.
Figure 18-22 illustrates two kinds of slicing devices, in order to the vegetable material that swims in the liquid medium is cut into slices.
Device shown in Figure 180 is specially adapted to cultivate with section from the gul preparation.This device has the casing 74 that the most handy transparent material is made, the housing 75 of built-in motor one class drive unit (not shown), the rotating shaft 73, upper cutter 71 and the lower cutter 72 that are rotated by drive unit.Casing 74 preferably can unload from housing 75.Lower cutter 72 is preferably straight shape blade, and upper cutter is preferably the cambered blade that is bent upwards as shown in figure 18. Cutting knife 71 and 72 is fixed on the different parts of axle 73, preferably is mutually 180 ° of angles.
In the course of work of device shown in Figure 180, vegetable material and water, buffer solution or culture medium one class I liquid I medium are introduced in the casing 74, with required rotating speed and rotation of required cycle cutting knife 71 and 72.For example, the rotating speed in preparation rose when section cutting knife 71 and 72 is 50-10000rpm, is preferably 500-50000rpm, and rotation period is 0.1-100 second, is preferably 0.5-20 second.
Figure 19 illustrates the another kind of device in order to preparation plant section in liquid medium.This device has casing 81, be contained in the cutting knife 86 on the hub body 85 of axle 84, and buffer board 89.On this device, also be provided with outlet 92 and inlet 91,, in addition, also be provided with the air hose 94 of band air cleaner 93 respectively in order to the section in the unloading liquid medium with in the vegetable material feeder.Cutting knife 86 has two cutters and two bottom knife.Two cutters are mutually 180 ° of angles and stretch out from hub body 85, and two bottom knife also are mutually 180 ° of angles, stretch out as shown in figure 21 from hub body 85 at an angle of 90 with cutter.Figure 20 is the zoomed-in view of cutting knife 86 shown in Figure 19.As shown in figure 20, bottom knife 86 is horizontally fixed on the hub body 85, but 86 of cutters are fixed thereon at angle with horizontal plane.It should be noted that: this angle can change, and, each cutter 86 is clipped in two semicircle spares 88 for this reason, this sub-assembly is inserted in the hole of hub body 85, so that cutter 86 is fixed on the hub body 85 shown in Figure 20 and 22 again.Blade 86 and semicircle spare 88 usefulness are fixed on the hub body 85 from the screw that hub body 85 end faces insert.
In the course of work of device shown in Figure 19, by 91 introduced plants material and the liquid medium of entering the mouth, rotate cutting knife by flexible axle 83, axle 84 and hub body 85 with electromotor one class drive unit 82, also be rotating blade 86.The buffer board 89 disturbance casings 81 interior flowing liquid media that generally are contained in relative both sides in the casing 81 are cut into slices in order to 86 pairs of vegetable material of cutting knife.After section finishes, the section in the liquid medium is drawn off from exporting 92.In the course of the work, air is introduced casing 81 by air cleaner 93 and air hose 94, so that the casing 81 inner germ-free conditions that keep.Introducing the air of casing 81 also uses so that the section in the liquid medium is transported in the container (not shown) of using for the cultivation plant tissue by exporting 92.
Be easy to prepare a large amount of sections by the present invention, and plant tissue polluted and necrosis in order to the cultivation plant tissue.This method can be in order to from preparation sections such as some part of whole plant or plant such as flower, leaf, stalk, the tip, napiform root, stem tuber, corpus callosums, and section can be cultivated to generate useful material or whole newborn plant to gained.
Now the present invention will be described by example.
Implement 1
Adopted device shown in Figure 1 in this example, the gap adjustment between blade 6 and the plane, disk 3 surperficial place is 2mm.With cover 10 whole device is encapsulated in the aluminium foil, in high steam, carries out disinfection again.Then, be that the seedling point of passing through of 5-8mm is cultivated the aseptic napiform root of gained Lilium longiflorum and put inlet 9 into 60 diameters, the pressing plate (not shown) is placed on the napiform root in the inlet, a weight is placed on the pressing plate, making the pressure that is added on the napiform root is 400g/cm
2The rotating speed that is fixed on the blade on the disk is 350rpm.Section cultivated for 15 week in capacity is 1 liter small-sized fermentation device, meanwhile feed pure oxygen therein, thereby had obtained the napiform root that 2360 diameters are about 3mm.
Example 2
Adopted the device identical, but the gap adjustment between blade and the plane, disc surfaces place is 2mm with example 1.
With one section diameter is 28mm, and long potato for 65mm is put into inlet 9, and the pressure that is added on this section potato is 45g/cm
2Rotating speed is changed between 20-400rpm.With the irrelevance of the thickness of different rotating speeds gained section and slice thickness as shown in figure 23.Figure 23 shows: be the section that has obtained the thickness homogeneous between the 100-300rpm at rotating speed.
Example 3
Repeat example 2 described operations, but rotational speed regulation is 350rpm, is added in pressure on this section potato at 50g/cm
2-400g/cm
2Between the change.As shown in figure 24, be 200g/cm at pressure
2-350g/cm
2Between obtained the section of thickness homogeneous.
Example 4
Adopted the section of device shown in Figure 4, obtained similar of the result who obtains and example 1 with preparation Lilium longiflorum napiform root.
Example 5
Once the other rose of sweetheart rose, Marie Antoinette was put in the Marashige Scooge medium that is contained in the glass blake bottle and cultivated, medium contains 30% sucrose, and be added with the benzyladenine (pH5.7) of 0.3 μ m, make these other flower buds under 25 ℃, the cultivation of carrying out for four weeks under the illumination condition of 16 hours bright cycles (400lux) and 8 hours dark cycles is to obtain newborn plant.Adopt device shown in Figure 180, in its capacity is 0.4 liter casing 74, added plant and 0.3 liter of cultivation liquid medium of 100 new lives.Cutting knife is cut into slices to plant with the rotating speed of 2000rpm, and in 10 seconds, finished the section operation.Section put in the above-mentioned medium that has added 1 μ m benzyladenine cultivates, in this medium, under the condition of bubbling air, to slice culture four weeks, like this, obtained 3.24 plants from every original newborn plant.
Reference example
Plant to 100 obtained in example 5 new lives has carried out manual section with scalpel, cultivates by 5 pairs of sections of example.
In 5 hours, finished the section operation, obtained 2.28 plants from every original newborn plant.
Example 6
Once 2 liters of culture mediums were put into device shown in Figure 19, in this device, bottom knife is horizontally fixed on the propeller boss, and cutter then is fixed on the propeller boss with respect to 15 ° of angles of one-tenth, plane whole device was sterilized under 121 ℃ 15 minutes with steam under pressure.After the cooling 100 sweetheart rose seedling 91 have been sent in the casing 81 by entering the mouth, cutting knife was rotated under rotating speed 1200rpm 90 minutes.As a result, the seedling more than 95% is carried out sharp section, obtained the other flower bud of about 400 3-4mm, these other flower buds are cultivated, obtained 350 plants.
Example 7
Repeat example 6 described operations, but cutter 20 ° of angles have been inclined relative to horizontal.Like this, obtained the other flower bud of 260 3-4mm.
Claims (36)
1, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is cut into plant section;
It is characterized in that vegetable material is infeeded the mechanical slicing device of sterilization or sterilization with this vegetable material of sliding cutting, and then preparation aftergrowth section, said apparatus has the disk with one or more elongated openings, the a certain tangent line of these openings and disk is provided with at angle, by drive unit disk is rotated, this device also has one or more blades that are fixed on the disk, the blade of each blade be positioned at each opening above, between the plane at the surperficial place of blade, leave the gap on blade and the disk, be 1 to 4mm near the distance between the plane, surperficial place of blade on the blade of blade and the disk, the rotating speed of this disk is 100 to 400rmp, and the pressure that the plant of need section is pressed to blade is 0 to 500g/cm
2, kind, the classification of plant body and the different parts of plant of the plant that needs section depended in the selection of these conditions.
2, in accordance with the method for claim 1, it is characterized in that: blade is provided with radially and is curved.
3, in accordance with the method for claim 1, it is characterized in that: blade is fixed on the disk with screw, and detachable.
4, in accordance with the method for claim 1, it is characterized in that: form a gap with pad between blade and the disk.
5, in accordance with the method for claim 1, it is characterized in that: disk is contained in the container, and disk and container are made by metal, therefore, can carry out heating disinfection to whole device.
6, according to the described method of claim 1, it is characterized in that: napiform root or stem tuber to plant are cut into slices.
7, in accordance with the method for claim 1, it is characterized in that: blade becomes an angle setting less than the right angle with a certain tangent line of disk.
8, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to plant sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that the cutting knife that vegetable material is pressed to the mechanical slicing device that the cutting knife of mechanical slicing device of sterilization or sterilization maybe will sterilize or sterilize is pressed to vegetable material cuts into slices to prepare aftergrowth, slicing device has some guide plates parallel to each other and is contained between the guide plate and parallel with it some cutting knifes, cutting knife rotatable, reciprocating or the vibration, thereby when vegetable material between guide plate by the time its cutting is in blocks.
9, in accordance with the method for claim 8, it is characterized in that: slicing device also has the casing that guide plate and cutting knife are equipped with in inside, and this device also has pressing plate, and pressing plate is pressed to cutting knife with vegetable material, when vegetable material was passed through between guide plate, cutting knife was in blocks with its cutting.
10, in accordance with the method for claim 8, it is characterized in that: each cutting knife is the disk that has blade on the periphery, and each disk is fixed on the rotating shaft, and rotating shaft passes the hole on each guide plate, rotates this axle with drive unit.
11, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that with vegetable material press to sterilization or sterilization mechanical slicing device cutting knife or the cutting knife of the mechanical slicing device of sterilization pressed to vegetable material with the section of preparation aftergrowth, cutting knife has fixed cutter body and movable cutter hub, fixed cutter body has that some two surfaces by fixed cutter body are intersected and the blade that forms, movable cutter hub has that some two surfaces by movable cutter hub are intersected and the blade that forms, wherein, sliding-contact is done on a surface of movable cutter hub and a surface of fixed cutter body, therefore, movable cutter hub rotates with respect to fixed cutter body, reciprocating or vibration and will to press to the vegetable material cutting of cutting knife in blocks.
12, in accordance with the method for claim 11, it is characterized in that: slicing device also has inside and the casing of cutting knife is housed and vegetable material is pressed to the pressing plate of cutting knife, and fixed cutter body is fixed on the casing.
13, the method for preparation in quantity energy aftergrowth histologic section in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned plant is being cut into plant section;
It is characterized in that the cutting knife of vegetable material being pressed to the mechanical slicing device that the cutting knife of the mechanical slicing device of sterilization maybe will sterilize or sterilize presses to vegetable material, slicing device has a pair of roller parallel to each other at least, gapped between the roller, in order to transmit vegetable material, downstream at roller then is provided with some cutting knifes, cutting knife rotates or reciprocating around an axle that is parallel to roller axis, will be in blocks by the vegetable material cutting that roller transmits.
14, in accordance with the method for claim 13, it is characterized in that: cutting knife is some disks that have blade on periphery, is fixed on the rotating shaft that is rotated by drive unit.
15, in accordance with the method for claim 13, it is characterized in that: the periphery of each roller is provided with some grooves, and the blade of each disk embeds groove but not contact with it.
16, according to the described method of claim 13, it is characterized in that: this device also has pressing plate and the inner casing that roller, cutting knife and pressing plate are housed of vegetable material being pushed to roller.
17, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that the cutting knife that vegetable material is pressed to the mechanical slicing device that the cutting knife of mechanical slicing device of sterilization or sterilization maybe will sterilize or sterilize is pressed to vegetable material cuts into slices to prepare aftergrowth, slicing device has a pair of parallel to each other and leave the roller of spacing in the centre and be arranged on some cutting knifes between the roller at least, each cutting knife is fixed on the axle that is parallel to roller axis, and this axle rotates with drive unit.
18, in accordance with the method for claim 17, it is characterized in that: this device also has the pressing plate of vegetable material being pushed to roller, and roller is equipped with, the casing of cutting knife and pressing plate in inside.
19, in accordance with the method for claim 17, it is characterized in that the roller periphery is provided with some grooves, the blade of blade embeds groove, but not contact with it.
20, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that the cutting knife of mechanical slicing device of vegetable material being pressed to sterilization or sterilization presses to vegetable material, with preparation aftergrowth tissue, slicing device has two rollers at least, one of them roller has some grooves on its periphery surface, another roller has some grooves on its periphery surface on the plane different with the groove of relative roller, be equipped with a cutting knife that stretches out from bottom land in each groove.
21, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that the cutting knife of mechanical slicing device of vegetable material being pressed to sterilization or sterilization presses to vegetable material, slicing device has some parallel to each other or be latticed cutting knife, the blade of each cutting knife is towards same direction, and each cutting knife also can be provided with radially, and the blade of each cutting knife is towards radiation center.
22, according to the described method of claim 21, it is characterized in that: each cutting knife is provided with radially, makes the blade of each cutting knife be the convexity setting.
23, according to claim 21 or 22 described methods, it is characterized in that: this device also has the pressing plate of plant being pressed to cutting knife, and hold-down plate surface should make this surface of pressing plate contact with the blade of whole blades when pressing plate is pressed in each blade to the surperficial institute tool shape of cutting knife.
24, the method for preparation in quantity plant tissue slice of energy aftergrowth in Plant Tissue Breeding comprises the steps:
Vegetable material to vegetable material sterilization or preparation sterilization;
In the device of sterilization or sterilization, above-mentioned vegetable material is being cut into plant section;
It is characterized in that preparing the liquid medium that contains through sterilization or sterilization vegetable material, and use the cutting knife cutting plants material of machine cuts device through sterilization or sterilization, this cutting knife by rotate, reciprocating or vibration cuts into plant section with vegetable material, the rotating speed of cutting knife is 50 to 50000rpm, is preferably 500 to 1000rpm.
25, according to the described method of claim 24, it is characterized in that: this method adopts claim 1,8,11,13,17,20 and 21 described devices, and with this device serial connection in the pipeline that has liquid medium therefrom to circulate.
26, according to the described method of claim 24, it is characterized in that: vegetable material is the plant stalk stem with other flower bud.
27, according to the described method of claim 24, it is characterized in that: in the device that this method adopted, cutting knife is fixed on the upper and lower of rotating shaft, makes this rotation with drive unit.
28, according to the described method of claim 27, it is characterized in that: lower cutter has one or morely makes the radial blade that stretches out from axle.
29, according to the described method of claim 27, it is characterized in that: lower cutter is horizontally fixed on the axle, and upper cutter and horizontal plane are fixed on the axle at angle, and this angle can change.
30, in accordance with the method for claim 27, it is characterized in that: lower cutter is horizontally fixed on the axle, the curved vertical setting of upper cutter.
31, in accordance with the method for claim 27, it is characterized in that: the blade of upper cutter is clipped in two semicircle spares, and this assembly is inserted in the hole of propeller boss, so that the blade of upper cutter is fixed on the axle.
32, according to the described method of claim 27, it is characterized in that: cutting knife and axle are contained in the casing, and one or more buffer boards are housed in casing.
33, according to the described method of claim 27, it is characterized in that: the variable-angle between buffer board and the box body wall moves.
34, according to the described method of claim 32, it is characterized in that: on roof box, be provided with the inlet of sending vegetable material to, this inlet outer incline setting.
35, according to the described method of claim 27, it is characterized in that: vegetable material is the stalk stem with other flower bud, cuts into slices to be other flower bud that separates or the stalk stem section with other flower bud.
36, according to the described method of claim 27, it is characterized in that: in the method, adopted the gul material.
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31119687 | 1987-12-08 | ||
| JP311196/87 | 1987-12-08 | ||
| JP108029/88 | 1988-04-30 | ||
| JP153556/88 | 1988-06-23 | ||
| JP153586/88 | 1988-06-23 | ||
| JP276530/88 | 1988-10-31 | ||
| JP278456/88 | 1988-11-01 | ||
| JP27845688 | 1988-11-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1034500A CN1034500A (en) | 1989-08-09 |
| CN1016857B true CN1016857B (en) | 1992-06-03 |
Family
ID=26552886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88108381 Expired CN1016857B (en) | 1987-12-08 | 1988-12-08 | Method of preparation of inoculum sections for plant tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1016857B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119632A (en) * | 2010-12-29 | 2011-07-13 | 刘晶 | Quick inoculation device for solid strains of edible mushroom |
| CN104029234B (en) * | 2014-06-18 | 2015-11-25 | 山东省果树研究所 | A kind of tissue culture seeding stem segment cutter sweep and application thereof |
-
1988
- 1988-12-08 CN CN 88108381 patent/CN1016857B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN1034500A (en) | 1989-08-09 |
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Patentee after: Mitsui Chemical Industry Co., Ltd. Patentee before: Mitsui Petrochemical Industries, Ltd. |
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