CN101655476B - Electrochemical measuring method of ferric iron content - Google Patents
Electrochemical measuring method of ferric iron content Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 38
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 title claims abstract description 33
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 238000012360 testing method Methods 0.000 claims abstract description 40
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- 238000004458 analytical method Methods 0.000 abstract description 16
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- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 5
- 238000002386 leaching Methods 0.000 description 5
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- 230000007613 environmental effect Effects 0.000 description 4
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
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- 239000012535 impurity Substances 0.000 description 4
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- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
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- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The invention relates to an electrochemical measuring method of ferric iron content, and an electrochemical three-electrode testing system and a constant-current anode stripping differential timing potentiometric method are adopted to carry out the measurement. The electrochemical measuring method is characterized by comprising the following steps: reducing ferric iron by hydroxylamine hydrochloride, extracting chelate Fe-[(C12H8N2)3]2+ formed by the chelation of 1,10-orthophenanthrolene into propylene carbonate organic solvent and enriching on the surface of a working electrode, obtaining a dT/dE-E differential curve by recording a function of the change of an electric potential E on the working electrode along with the testing time T and carrying out the differential processing on the function, and calculating the ferric iron content according to the height dT/dE (sV-1) of an iron response peak that is generated when an anode stripping electric potential on the differential curve is plus 400mV to plus 500mV. The invention has simple process, convenient operation, high sensitivity and low operation cost and can carry out analysis and testing on micro or trace ferric iron in natural water quality.
Description
One, technical field
The present invention relates to a kind of analysis determining method of inorganic ions, particularly electrochemical analysis method, specifically a kind of electrochemical determination method of ferric iron content.
Two, technical background
Iron is one type of important native metal element of occurring in nature, is distributed widely in soil, mineral, water environment, biosystem, and even in the food chain.Iron can with multiple chemical combination attitude (0, II, III) and the form of organic and inorganic thing be present in the physical environment, and wherein common with Fe (III).In life science; Iron is one of animal and plant and human essential trace element, the aspect such as anticancer of the activation of its growth biosome, metabolism, nerve conduction, enzyme, cell play a significant role [H.Hasegawa, T.Maki; K.Asano; Et al.Analytical Sciences, 2004,20 (1): 89-93; D.S.Yu, S.P.Liu, Z.F.Liu, et al.Chinese Journal of Geochemistry, 1995,14 (2): 134-139].Yet; The biological function of ferro element only limits to (10～20 mg/day, China's drinking water standard regulation iron content must not surpass 0.3 mg/litre) in certain proper concentration, is higher than the scope of this suitable concn; To cause symptoms such as animal and plant iron poisoning and neurological disorders; Also can make to be prone to breed bacteria in the potable water, increase the turbidity of water, produce special odor; And be lower than this concentration range; Will cause iron deficiency or hypoferric anemia; Can the body function of animal and plant be caused damage equally [Ministry of Health of the People's Republic of China. drinking water sanitary standard (GB/T 5749-2006). Beijing: China Standard Press, 2007 (4): 150-163; S.Morals, G.S.Carvalho, J.P.Sousa.Electroanalysis, 1997,9 (10): 791-795].
Iron is as indispensable trace element in a kind of human body; Be again a kind of intoxicating element simultaneously; Iron and compound thereof have all received very big concern in recent years to the influence of industrial and agricultural production and daily life, the utilization, biological function, biochemical reaction in biosome, the transfer in environment and biosystem etc.Therefore; Trace and trace iron ions are significant in analysis and control nature water, potable water, biosystem, environment and the food, have developed a large amount of analytical approach [B.S.Sherigara, Y.Shivaraj about iron mensuration at present; R.J.Mascarenhas; Et al.Electrochimica Acta, 2007,52 (10): 3137-3142; W.Martinotti, G.Queirazza, A.Guarinoni, et al.Analytica Chimica Acta, 1995,305 (1): 183-189].As the instrumental neutron activation analysis method (INAA) in potassium dichromate method, volumetric method, chemoluminescence method, the conventional analysis and with all kinds atomic absorption spectrography (AAS) of other analytical approach coupling (HG-AAS, ET-AAS), inductively coupled plasma-atomic emission spectrometry (ICP-AES), high-pressure liquid phase-ultraviolet spectrophotometry (HPLC-UV), hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS), inductively coupled plasma mass spectrometry method (ICP-MS), various forms of electrochemical analysis methods etc.
In above-mentioned measuring technology and method, most of content analysis mensuration to iron needs to cooperate other pertinent instruments, equipment, technology or increases the pre-treatment process.Thereby its routine analyzer is complicated, analytical cycle is long, operating cost is high; Owing to these technology, method are based on breadboard analytical test, can not be used for the analysis of field, on-the-spot sample, thereby its range of application receives very big restriction simultaneously.Yet electroanalysis chemical process and technology like capillary electrophoresis, polarography, voltammetry, timing potentiometric method etc., are widely used in the test and the Determination of Different Valence States of trace and trace metal ion.Different with electrochemical techniques such as polarography and voltammetries; Potentiometric method is used on analysis principle during difference gauge is the steady current rather than the scanning electromotive force of low metering, changes with the differentiation function of test duration (T) through the electromotive force on the working electrode (E) that (dT/dE → E) measures metallic ion.Therefore; During difference gauge potentiometric method have quick, simple to operate, the automation equipment degree is high, highly sensitive, selectivity is good, detectability is low, the analytic process pollutant is few, receive organism disturb little, can measure multiple element simultaneously and the different valence state of identity element such as is analyzed at characteristic, and need not remove the oxygen in the test solution in the potentiometric method analytic process during difference gauge, the step that can further simplify the operation with improve analysis efficiency; [S.C.Chuanuwatanakul is widely used; W.J.Dungchai, O.Chailapakul, et al.Analytical Sciences; 2008,24 (5): 589-594].Before the present invention makes; A kind of ferric assay method is disclosed among Chinese invention patent CN 1811392.7 and the CN 1920537.7; Adopt respectively computing machine colourity identification and visible spectrophotometry [also can be referring to S.Bhattacharya, S.K.Roy, A.K.Chakraborty.Talanta; 1990,37 (11): 1101-1104]; Or be employed in Cathodic Stripping Voltammetry [C.M.G.van den Berg, M.Nimmo, the O.Abollino on the DME; Et al.Electroanalysis, 1991,3 (5): 477-482]; Cathodic Stripping Voltammetry on the solid mercury film electrode [A.P.Aldrich and C.M.G.van den Berg.Electroanalysis, 1998,10 (6): 369-373], timing electromotive force leaching [R.D.Riso; B.Pernet-Coudrier, M.Waeles, et al.Analytica Chimica Acta; 2007,598 (7): 235-241]; Or be employed in glassy carbon electrode [J.F.van Stadenand M.C.Matoetoe.Analytica Chimica Acta, 1998,376 (11): 325-331] or chitin modified glassy carbon electrode [G.Lu; X.Yao, X.Wu, et al.Microchemistry Journal; 2001,69 (7): 81-85] anodic stripping voltammetry on, potentiometric method [D.Jagner, L.Renman; S.H.Steffansdottir.AnalyticaChimica Acta, 1993,281 (3): 305-311] etc.But because the hypertoxicity of mercury and compound itself thereof and to the harmfulness of ecologic environment, its application in recent years is very limited, and while iron reappearance on mercury class electrode is relatively poor; Influenced its analytical performance [M.D.Gelado-Cabakkero; J.J.Hemandez-Brito, J.A.Herrera-Melian, et al.Electroanalysis; 1996,8 (11): 1065-1071; C.Hua, D.Jagner, L.Renman.Talanta, 1988,35 (8): 597-600].And the anode leaching poor sensitivity on the glass carbon class electrode, and situation such as multimodal appear, the specified rate analysis has also brought trouble [P.B.Zhu, T.Nakamura, K.Izutsu.Electroanalysis, 1990,2 (1): 75-79; Frazier W.M.Nyasulu, Horacio A.Mottola.Journal of ElectroanalyticalChemistry, 1988,239 (1-2): 175-186].The assay method and the technology of the relevant iron content that therefore, exploitation is environmentally friendly, nontoxic, highly sensitive, reappearance is good, selectivity good, detectability is low more and more come into one's own.
Three, summary of the invention
The objective of the invention is deficiency to the prior art existence; Provide that a kind of technology is simple, environmental friendliness, the highly sensitive ferric method of detection, technical matters to be solved is that ferric iron is handled when making it to be applicable to anode stripping difference gauge potentiometric method and can be carried out assay determination to trace in the sample or trace ferric iron.
Technical scheme of the present invention is that potentiometric method (CCSCP) is measured when adopting galvanochemistry three electrode test systems and continuous current anode stripping difference gauge; Difference with the prior art is a ferric iron through oxammonium hydrochloride reduction, 1, and 10-phenanthrolene chelatropic reaction forms chelate Fe-[(C
12H
8N
2)
3]
2+Be extracted in the propylene carbonate solvent, chelate is enriched in the surface that electrode is made in metal working under-1.0 μ A～-7.0 μ A electric currents, measure then, and its stripping sweep current is+1.0 μ A～+ 6.0 μ A, and sweep speed is 20mV s
-1Frequency is 20Hz; The static stand-by period is 10s～30s; Through electromotive force E on the writing task electrode with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E, and according on the differential curve anode stripping current potential+400mV～+ iron peak value of response height dT/dE (the s V that produces during 500mV
-1) the calculating ferric iron content.
After measuring end, metal working is carried out galvanochemistry as electrode clean,, carry out new enrichment, testing process then to remove residual reductive agent, dentate, iron and chelate thereof and other impurity.Galvanochemistry is cleaned the oxidation current that adopts+5.0 μ A and is cleaned 30s～120s.
Specific operation process is following:
Step 1 is in its course of work, with 5.0mmol L
-1Sodium acetate as damping fluid, 0.2～5.0mol L
-1NaCl be supporting electrolyte, 20.0～400.0mmol L
-1Oxammonium hydrochloride be reductive agent, 2.0～70.0mmol L
-11, the 10-phenanthrolene is a dentate;
Potentiometric method (CCSCP) during described continuous current anode stripping difference gauge, its stripping sweep current is+1.0 μ A～+ 6.0 μ A, sweep speed is 20mV s
-1Frequency is 20Hz; The static stand-by period is 10s～30s; Through electromotive force E on the writing task electrode with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E, and according on the differential curve anode stripping current potential+400mV～+ iron peak value of response height dT/dE (the s V that produces during 500mV
-1) the calculating ferric iron content.
The oxidation current that electrodes use+5.0 μ A are made in described metal working carries out the oxidation cleaning of 30s～120s.
The present invention is in galvanochemistry three electrode test systems; The employing gold electrode is a working electrode; Ferric ion is reduced the agent oxammonium hydrochloride and is reduced to ferrous ion in the solution; With sodium-acetate buffer regulator solution pH value about 4.0～5.0, ferrous ion and dentate 1,10-phenanthrolene reaction forms orange-red chelate Fe-[(C
12H
8N
2)
3]
2+, then with chelate the Fe-[(C in carbonic allyl ester extraction and the separation solution
12H
8N
2)
3]
2+This chelate is in galvanochemistry anode process in leaching subsequently; When anode stripping current potential be+400mV～+ during 500mV, produce a very sensitive peak value of response, ferric content is directly related in its peak heights and the sample; Thereby realize ferric assay determination, also can be directly or be used for natural water-like and the inorganicization artifact organic sample trace or the ferric mensuration of trace of acidifying behind the mark-on.The assay method of this ferric iron content also can adopt working electrode+and oxidation that the oxidation current of 5.0 μ A carries out 30s～120s cleans, and removes residual reductive agent, dentate, iron and chelate thereof and other impurity etc., carries out new reduction enrichment process then.
Compared with prior art, the present invention has advantage:
(1) because employing replaces the violent mercury electrode of toxicity with gold electrode, thereby the present invention has environmental friendliness, nontoxic characteristics.
(2) because the present invention adopts chelate Fe-[(C
12H
8N
2)
3]
2+The method of reduction enrichment; Ferrous ion during each anode stripping in the chelate is oxidized to ferric iron and can be reduced once more by oxammonium hydrochloride; Ferrous ion continuous current oxidation stripping once more on working electrode that reduction obtains; Improved the sensitivity of test, also effectively avoided the saturated reaction of iron to a certain extent, enlarged the concentration determination scope of iron at electrode surface; Simultaneously owing to adopt the 5mL carbonic allyl ester fully to extract chelate the Fe-[(C in the 20mL solution
12H
8N
2)
3]
2+, and get 2mL carbonic allyl ester extract as test solution, concentrated the concentration of iron relatively, reduced the influence of historical residual impurity etc., improved the reappearance and the accuracy of test.
(3) because the present invention utilizes ferrous iron and 1, chelate the Fe-[(C that the 10-phenanthrolene forms
12H
8N
2)
3]
2+In the carbonic allyl ester organic phase, carry out the stripping of continuous current electrochemical oxidation; Through electromotive force E on the writing task electrode with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E, according on the differential curve in+400mV～+ near produce the 500mV peak value of response height dT/dE (sV
-1) measure ferric iron content, thereby have that enrichment time is short, highly sensitive, favorable reproducibility, characteristics that detectability is low, can carry out assay determination to trace in the sample or trace iron.Adopt suitable enrichment time, the present invention can directly measure iron content in the biological organism after the natural water-like after the acidifying, tap water, human hair and inorganicization, and technology is simple; Easy to operate; Cost is low, and is pollution-free, has broad application prospects.
Four, description of drawings
Fig. 1 be the technical scheme by the embodiment of the invention 1 record Fe (III) continuous current anode stripping difference gauge the time electromotive force characteristic spectrum;
Electromotive force collection of illustrative plates when Fig. 2 is the continuous current anode stripping difference gauge that Fe in the seawater (III) is recorded by the technical scheme of the embodiment of the invention 2;
Electromotive force collection of illustrative plates when Fig. 3 is the continuous current anode stripping difference gauge that Fe in the river (III) is recorded by the technical scheme of the embodiment of the invention 3;
Five, embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is further described:
Embodiment 1:
Potentiometric analysis appearance and galvanochemistry three electrode test systems when present embodiment adopts HOKUTO DENKO HAB-151 difference gauge; With the gold electrode is working electrode; Platinum filament is to the utmost point; Ag/AgCl is a reference electrode, ferric iron content in potentiometric method (CCSCP) specimen during with continuous current anode stripping difference gauge.Reduction enrichment and anode leaching condition are respectively: the enrichment electric current is-4.0 μ A, and enrichment time is 180s, and be 30s rest time; During test, anode stripping sweep current is+5.0 μ A, and sweep speed is 20mV s
-1, frequency is 20Hz.
Referring to accompanying drawing 1, curve 1 is to 5.0mmol L with said method among the figure
-1Sodium acetate+80mmol L
-1Oxammonium hydrochloride+15.0mmol L
-1Phenanthrolene solution is through comprising 0.2mol L
-1The result that the carbonic allyl ester extraction back test of TBAP obtains; Curve 2 is for using this method to 5.0mmol L
-1Sodium acetate+80mmol L
-1Oxammonium hydrochloride+10.0 μ g L
-1Fe (III) solution is through comprising 0.2mol L
-1The result that the carbonic allyl ester extraction back test of TBAP obtains; Curve 3 is for add 10.0 μ g L again in curve 1 described solution
-1Fe (III) through comprising 0.2mol L
-1After the carbonic allyl ester extraction of TBAP, test the result who obtains with same procedure.
Can find out by Fig. 1, at sodium acetate+oxammonium hydrochloride+end liquid of phenanthrolene existence and the 10.0 μ gL that do not comprise the dentate phenanthrolene
-1In Fe (III) solution, do not observe tangible anode stripping peak (curve 1,2); And when in end liquid, adding 10.0 μ g L
-1During Fe (III), obtain the characteristic response peak (curve 3) that a peak shape is good, belong to iron at the stripping current potential for+450mV place, its peak height is direct relevant with Fe (III) concentration.
Behind the EOT, the galvanochemistry of working electrode is cleaned the CURRENT OXIDATION that adopts+5.0 μ A clean 30s, remove residual reductive agent, dentate, iron and chelate thereof and other impurity etc., carry out new reduction enrichment process then.
Embodiment 2:
Potentiometric analysis appearance and galvanochemistry three electrode test systems when present embodiment adopts HOKUTO DENKO HAB-151 difference gauge; With the gold electrode is working electrode; Platinum filament is to the utmost point; Ag/AgCl is a reference electrode, and potentiometric method (CCSCP) during with continuous current anode stripping difference gauge is to adding the mensuration that the target seawater carries out ferric iron content.Reduction enrichment and anode leaching condition are respectively: the enrichment electric current is-5.0 μ A, and enrichment time is 180s, and be 30s rest time; During test, anode stripping sweep current is+5.0 μ A, and sweep speed is 20mV s
-1, frequency is 20Hz.
Fresh actual seawater sample is the micropore filter paper suction filtration of warp 0.45 μ m earlier.
Referring to accompanying drawing 2, earlier to containing 5.0mmol L
-1Sodium acetate, 1.0mol L
-1NaCl, 80mmol L
-1Oxammonium hydrochloride, 7.5mmol L
-1Liquid is through comprising 0.1mol L at the bottom of the 10mL of phenanthrolene
-1Test after the carbonic allyl ester extraction of TBAP, the result is shown by curve 4; In curve 4 solution, add 10mL sampling seawater after treatment, regulate and keep the equilibrium concentration of each component to be: 5.0mmol L
-1Sodium acetate, 1.0mol L
-1NaCl, 80mmol L
-1Oxammonium hydrochloride, 7.5mmolL
-1Phenanthrolene is again through comprising 0.1mol L
-1Test after the carbonic allyl ester extraction of TBAP, the result is shown by curve 5; Then, adopt standard addition method, with 2.0 μ g L
-1The concentration gradient of Fe (III) is carried out standard and is added, and obtains test curve 6～9 successively.Electromotive force E according to characteristic peak on the differential curve and peak heights value thereof, is used for the assay determination of iron with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E on the writing task electrode.
Can find out by Fig. 2, the actual seawater sample behind the mark-on, near the response peak peak shape+450mV is good, highly sensitive, and peak height is with the proportional increase of mark-on concentration.
Embodiment 3:
Press the method for testing of embodiment 2, to adding the mensuration that the target river carries out iron content.
Fresh actual river water sample is the micropore filter paper suction filtration of warp 0.45 μ m earlier.
Referring to accompanying drawing 3, earlier to containing 5.0mmol L
-1Sodium acetate, 1.0mol L
-1NaCl, 80mmol L
-1Oxammonium hydrochloride, 7.5mmol L
-1Liquid is through comprising 0.1mol L at the bottom of the 10mL of phenanthrolene
-1Test after the carbonic allyl ester extraction of TBAP, the result is shown by curve 10; In curve 10 solution, add 10mL sampling river after treatment, regulate and keep the equilibrium concentration of each component to be: 5.0mmol L
-1Sodium acetate, 1.0mol L
-1NaCl, 80mmol L
-1Oxammonium hydrochloride, 7.5mmolL
-1Phenanthrolene is again through comprising 0.1mol L
-1Test after the carbonic allyl ester extraction of TBAP, the result is shown by curve 11; Then, adopt standard addition method, with 5.0 μ g L
-1The concentration gradient of Fe (III) is carried out standard and is added, and obtains test curve 12～15 successively.Electromotive force E according to characteristic peak on the differential curve and peak heights value thereof, is used for the assay determination of iron with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E on the writing task electrode.
Can find out by Fig. 3, the actual river water sample behind the mark-on, near the response peak peak shape+450mV is good, highly sensitive, and peak height is with the proportional increase of mark-on concentration.
Embodiment 4:
Adopting the method for testing of embodiment 1,2 or 3, is 2.0 μ g L to known Fe (III) concentration
-1Analog sample 1, known Fe (III) concentration be 10.0 μ gL
-1 Analog sample 2 is through comprising 0.1mol L
-1Directly test after the carbonic allyl ester extraction of TBAP; Fresh actual sampling seawater, river through the micropore filter paper suction filtration of 0.45 μ m, are adopted 5.0mmol L earlier
-1The sodium acetate acidifying is again through comprising 0.1mol L
-1Directly test after the carbonic allyl ester extraction of TBAP; Fresh actual sampling seawater earlier through the micropore filter paper suction filtration of 0.45 μ m, is adopted 5.0mmol L
-1The sodium acetate acidifying adds 5.0 μ gL again
-1Fe (III) is through comprising 0.1mol L
-1Test after the carbonic allyl ester extraction of TBAP; Behind each EOT, adopt the oxidation current of+5.0 μ A to carry out 30s galvanochemistry to working electrode respectively and clean, test data and analysis result are referring to table 1.
Table 1
By the test data shown in the table 1, can see after the analysis, serve as to analyze quality control with the simulated water sample of concentration known, [in the standard solution test of Fe (III), its concentration range of linearity is 1.0～20.0 μ g L to the iron content of mensuration accurately and reliably
-1(linearly dependent coefficient r
2=0.9951); Sensitivity is 1.27s V
-1/ μ gL
-1Its method detects and is limited to 0.041 μ g L
-1, favorable reproducibility (R.S.D.%=3.25%; N=12)]; When the standard adding of actual seawater, river sample is analyzed; Linear relationship is good; The recovery of method is high, conventional metallic ion that exists such as Na (I), Mg (II), Al (III), Ca (II), Cu (II), Hg (II), Co (II), Sn (II), W (VI), Mo (VI), V (V), Ni (II), Cd (II), Pb (II), Mn (II), Zn (II), Sb (III), As (III), NH in the natural water
4 +, H
2PO
4 -, NO
3 -, SO
4 2-50～3000 times of under experiment condition, being higher than concentration of iron are not remarkable to the test result influence, thereby can realize direct analytical test to natural water.Simultaneously, to same fresh actual sampling seawater, it is 1.736 ± 0.02 μ g L that this laboratory adopts atomic absorption spectrography (AAS) (AAS) to measure ferric iron content
-1To same fresh actual sampling river, adopting ultraviolet spectrophotometry (UV) to measure ferric iron content is 7.339 ± 0.20 μ g L
-1Test shows, adopts the inventive method with approaching with iron content in AAS and the UV mensuration natural water.
The present invention has overcome the shortcomings such as environmental hazard of traditional mercury electrode, and technology is simple, and is easy to operate; Highly sensitive, operating cost is low, and is pollution-free; Can carry out analytical test to trace in the natural water or trace iron, it is in industrial and agricultural production, environmental protection; Biotechnology, health care, fields such as scientific research will have broad application prospects.
Claims (1)
1. the electrochemical determination method of a ferric iron content, potentiometric method is measured when adopting galvanochemistry three electrode test systems and continuous current anode stripping difference gauge, it is characterized in that: ferric iron is with 5.0mmol L
-1SAS is buffer solution, 0.2～5.0mol L
-1NaCl is through 20.0～400.0mmol L in the supporting electrolyte
-1Oxammonium hydrochloride reductive agent reduction back is with 1, and the reaction of 10-phenanthrolene forms chelate Fe-[(C
12H
8N
2)
3]
2+And with chelate Fe-[(C
12H
8N
2)
3]
2+Be extracted into and contain 0.02～0.50mol L
-1In the carbonic allyl ester organic solvent of TBuA perchlorate, chelate Fe-[(C
12H
8N
2)
3]
2+Under-1.0 μ A～-7.0 μ A electric currents, be enriched in the surface that electrode is made in metal working, measure then, its stripping sweep current is+1.0 μ A～+ 6.0 μ A, and sweep speed is 20mVs
-1Frequency is 20Hz; The static stand-by period is 10s～30s; Through electromotive force E on the writing task electrode with the variation function of test duration T and carry out differential and handle the differential curve that obtains dT/dE → E, and according on the differential curve anode stripping current potential for+400mV～+ the iron response peak height dT/dE that produces during 500mV calculates ferric iron content, dT/dE unit is sV
-1
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