CN101647513B - Method for producing feed protein by fermenting swill - Google Patents
Method for producing feed protein by fermenting swill Download PDFInfo
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- CN101647513B CN101647513B CN2009101447859A CN200910144785A CN101647513B CN 101647513 B CN101647513 B CN 101647513B CN 2009101447859 A CN2009101447859 A CN 2009101447859A CN 200910144785 A CN200910144785 A CN 200910144785A CN 101647513 B CN101647513 B CN 101647513B
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- swill
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- fermenting
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Abstract
The invention relates to a method for producing feed protein by fermenting swill, which comprises the steps of swill pretreatment, aspergillus niger seed culture, fermentation culture and post treatment. The invention is characterized in that the aspergillus niger seed liquid is inoculated in a swill culture medium for fermentation culture for 55-65 hours under the conditions that the temperature is 28-32 DEG C and the ventilation volume is 2-4L/min. The swill culture medium comprises 0.6-0.8 g/L swill, 12-16g/L carbon source and 10-14g/L nitrogen source, and the pH value is 6.0-6.5. The mycelia are separated, washed and dried after the fermentation process is finished; the yield is larger than or equal to 20g/L, and the content of the protein in the mycelia is 56.8-66%; and moreover, essential amino acids are relatively complete, thus products can be used as feed or food additives. The swill is clear and transparent and has no abnormal taste after being fermented, COD is reduced by 80-95%, BOD5 is reduced by 85-92.6%, and the pollution caused by direct discharge of the swill is greatly reduced.
Description
One, technical field
The present invention relates to a kind of method of comprehensive utilization of castoff of food and drink, specifically a kind of method of utilizing fermenting swill to produce forage protein.
Two, background technology
Swill is meant the percolate or the dilution of the surplus soup of locating to discard in dining room, restaurant etc. of leftovers.Mainly contain: organic matters such as starch, protein, grease, fibrid element.On the one hand, the organic matter content of swill is high, and poisonous and harmful substance content is few; But then, the perishable rotten smelly serious environment pollution of swill.
At present dining room, dining room are to abandon or collect the back directly as the feeds of pig, chicken, duck etc. to the processing method of swill and food and drink solid refuse, may make it infect infectious diseases common to human beings and animals serious harm healths such as aftosa, hepatitis B with feed poultry, domestic animal of swill.Directly abandon and can cause serious environmental to pollute and the wasting of resources, What is more, and the illegal businessman of minority does not stint and leads the grease that the consumer reclaims wherein into a trap and seek profit.Patent CN101129159A utilizes aspergillus niger and culture propagation waste slag of citrus manufacture order cell feed, feed mycoprotein content >=20%, cellulose degradation rate >=5%, but its single cell protein is mainly used in the field of fodder narrow in application range; The auxiliary water of water water standard during patent CN1824615A obtains with Wastewater Treated by Activated Sludge Process high concentration swill; Yet fail to make full use of the disposal cost that swill also need be born great number.
Three, summary of the invention
The present invention is intended to science processing and comprehensive utilization swill, and technical problem to be solved is through the fermenting and producing forage protein.
Thinking of the present invention is to utilize aspergillus niger as fermentation strain; Be seeded in the swill is to carry out liquid fermentation on the main culture medium of preparing; Both handled swill and suppressed harmful microbe and grown, the mycelium that the protein content of getting back is higher, post processing is simple and convenient than solid state fermentation in addition.
Technical scheme of the present invention comprises swill preliminary treatment, seed culture, fermented and cultured and post processing; Described swill preliminary treatment is exactly isolated by filtration and sterilization; Described seed culture is inoculated in black-koji mould under 28 ℃, to cultivate on the PDA culture medium exactly and produces spore, and sterilized water washes dilution makes spore concentration be about 1.4 * 10
8Individual/ml, described fermented and cultured is seeded in the swill culture medium spore suspension in 28～32 ℃, throughput 2～4L/min condition bottom fermentation exactly and cultivated 55～65 hours, carries out post processing after the fermentation ends, i.e. separating, washing and dry mycelium.
Described swill culture medium is to contain swill 0.6～0.8L, carbon source 12～16g, nitrogenous source 10～14g, pH6.0～6.5 in every liter of culture medium.
Described carbon source is selected from cornstarch or lactose or maltose or glucose or sucrose, preferably sucrose or glucose.
Described nitrogenous source is selected from potassium nitrate or yeast extract or ammonium sulfate or beef extract or ammonium nitrate or peptone, optimization protein peptone or ammonium nitrate.
This cultural method can get mycelium dry weight>=20g in every liter of culture medium; Record mycelium protein matter content 56.8～66% with triumphant formula nitriding; Detect through German sykam S-433D amino-acid analyzer, wherein essential amino acid is more complete, and organic matters such as total reducing sugar, fat and protein almost all are utilized in the nutrient solution of fermentation back; Swill is clarified after fermenting, transparent, free from extraneous odour, measures COD and BOD with reference to GB 11914-89 and GB 7488-87
5, COD reduces by 80～95%, BOD
5Reduce by 85～92.6%, reduced the directly pollution that causes of discharging of swill on largely.
This method has solved swill comprehensive utilization and problem of environment pollution caused effectively, and resulting black-koji mould body protein is comparatively pure, not only can be used as feed addictive, also can be used as food additives.
Four, the specific embodiment
Non-limiting examples is narrated as follows:
1, swill preliminary treatment
Get swill with six layers of filtered through gauze, the swill after the filtration is in 121 ℃ of sterilization 5min, and 4 ℃ of storages are subsequent use.
2, swill culture medium preparation
Get swill and add 1 premium on currency (swill content 0.66L/L) for 2 liters, add sucrose 42g (14g/L), ammonium nitrate 37g (12.6g/L), transfer pH6.2 with hydrochloric acid and the 0.1mol/L NaOH solution of 0.05mol/L, 121 ℃ of sterilization 20min, subsequent use.
3, seed culture
On being inoculated in aspergillus niger in the PDA culture medium; 28 ℃ of cultivations make it produce spore in the incubator; Wash with sterilized water then and be diluted in the triangular flask that bead is housed, place the 200r/min shaking table to break up spore and claim, contain 1.4 * 10 approximately in the suspension at the microscopically meter
8Spore/ml.
4, fermented and cultured and post processing
3L swill culture medium is dropped in the 5L fermentation tank, by 1.5% inoculum concentration inoculated aspergillus niger seed liquor, throughput 3L/min, 30 ℃ of bottom fermentations 60 hours.Suction filtration zymotic fluid after the fermentation ends, with distilled water washing 2～3 times, filtrating clarification, transparent, free from extraneous odour, COD reduces by 90.2%, BOD
5Reduce by 90%, reduced swill and directly discharged the pollution that causes.
Collect mycelium and dry to constant weight for 60 ℃, about 64g, plastic bag packaging is put aeration-drying place storage, does not have bad smell, appearance white or little yellow, protein content 64% and essential amino acid are more complete.
Claims (1)
1. method of utilizing fermenting swill to produce forage protein; Comprise swill preliminary treatment, the cultivation of black-koji mould seed, fermented and cultured and post processing; It is characterized in that: described swill preliminary treatment be swill with six layers of filtered through gauze, the swill after the filtration in 121 ℃ the sterilization 5min; It is exactly aspergillus niger strain to be inoculated on the PDA culture medium to cultivate down in 28 ℃ produce spore that described black-koji mould seed is cultivated, and sterilized water washes dilution, and to make spore concentration be 1.4 * 10
8Individual/mL; Described fermented and cultured is black-koji mould seed liquid to be seeded in the swill culture medium in 28～32 ℃, throughput 2～4L/min condition bottom fermentation cultivated 55～65 hours; Described swill culture medium is for containing swill 0.5～0.8L/L, carbon source 12～16g/L, nitrogenous source 10～14g/L, pH6.0～6.5; Described carbon source is selected from sucrose or glucose, and described nitrogenous source is selected from peptone or ammonium nitrate; Described post processing is meant separating, washing and dry mycelium after the fermentation ends.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101447859A CN101647513B (en) | 2009-09-04 | 2009-09-04 | Method for producing feed protein by fermenting swill |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101447859A CN101647513B (en) | 2009-09-04 | 2009-09-04 | Method for producing feed protein by fermenting swill |
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| CN101647513A CN101647513A (en) | 2010-02-17 |
| CN101647513B true CN101647513B (en) | 2012-06-13 |
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| CN2009101447859A Expired - Fee Related CN101647513B (en) | 2009-09-04 | 2009-09-04 | Method for producing feed protein by fermenting swill |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461648B (en) * | 2013-09-23 | 2015-01-21 | 精晶药业股份有限公司 | Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae |
| CN105016477A (en) * | 2014-04-16 | 2015-11-04 | 上海理工大学 | Technology for processing soybean wastewater by utilization of filamentous fungi |
| CN105767508A (en) * | 2016-03-15 | 2016-07-20 | 天津生态城环保有限公司 | Protein feed production method by mixing kitchen waste and vinegar residue |
| CN107319106A (en) * | 2017-09-05 | 2017-11-07 | 云南省普洱市思茅厚普饲料有限公司 | A kind of method that finishing pigs biological feedstuff is produced by primary raw material solid anaerobic digestion of swill |
| CN117844642B (en) * | 2024-03-05 | 2024-07-05 | 北京林业大学 | Method for purifying kitchen sewage by combining algae and bacteria |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338217A (en) * | 2000-07-18 | 2002-03-06 | 山元正博 | Composition for livestock food and production thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338217A (en) * | 2000-07-18 | 2002-03-06 | 山元正博 | Composition for livestock food and production thereof |
Non-Patent Citations (2)
| Title |
|---|
| Qunhui Wang et al..Glucoamylase production from food waste by Aspergillus niger under submerged fermentation.《Process Biochemistry》.2008,第43卷 * |
| 石磊 等.食物废弃物培养菌体蛋白的菌种筛选与参数优化研究.《化学世界》.2002,(第S1期), * |
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