CN101647471A - Novel application of salicornia europaea and root extractive thereof - Google Patents
Novel application of salicornia europaea and root extractive thereof Download PDFInfo
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Abstract
The invention discloses novel application of salicornia europaea and a root extractive thereof. The salicornia europae (Salicornia europaea L.) and the root extractive thereof provided by the invention are used for inhibiting the algae growth causing red tide and inhibiting the red tide. The algae causing the red tide is selected from at least one of skeletonema costatum, prorocentrum donghaienseand prorocentrum minimum. The method for preparing the root extractive of the salicornia europae comprises the following steps: using an organic solvent as an extractant, and extracting from the rootof the salicornia europaea, wherein the organic solvent is selected from any one of petroleum ether, chloroform, methanol and normal butanol. The result of the test shows that four different root extractives of the salicornia europaea can strongly inhibit the growth and the photosynthesis of three kinds of algae; for different algae, the allelopathy strength is different; for the skeletonema costatum, the allelopathy of the petroleum ether phase extractive is the strongest, and IC50 is 1.633 mg/L; for the prorocentrum donghaiense, the function of the chloroform phase extractive is the strongest, and IC50 is 13. 799 mg/L; and for the prorocentrum minimum, the allelopathy is improved along with the increasing of the polarity of the extractive, the function of the methanol phase is the strongest, and IC50 is 1.138 mg/L.
Description
Technical field
The present invention relates to the new purposes of salicornia europaeal and root extract thereof.
Background technology
Red tide is one of international community's marine environment problem of paying close attention to jointly, being badly in need of solving.Since the nineties in last century, the characteristics that present " high-frequency, multiple class, fast pace, " on a large scale of China's red tide, sustainable development to coastal waters ecotope and aquatic products industry causes great threat, endangers tourism development and human beings'health simultaneously.Adopting effective red-tide control measure is one of important content of red tide research.Chinese scholars proposes to comprise utilizes loess, clay, copper sulphate and some to administer red tide as the biologic product of a bacterium and a viral class.Though these methods may effectively but can cause long-term destruction in various degree to environment in a short time.Recent study discovery tangleweed can be secreted the gram living beings that suppress red tide plankton, the gram living beings of some of them biologically active are by the extraction of success and purifying, and be applied to the control and the management (Jin of red tide, Q., S.Dong, et al. (2005) .European Journal of Phycology 40 (1): 31-37.).Yet, because tangleweed exists season of growth property, fouling organism to adhere to and problem such as suspended particulate substance covering, seriously restrict its gram and given birth to the performance (Zhu of effect, B.I.N., C.M.Mayer, et al. (2007) .Journal of Aquatic Plant Management 45:71-76.).Therefore, find both to be adapted at growing in the briny environment, the plant that has higher reparation value and economic worth again is extremely urgent.The ocean beach plant that receives much concern in recent years is applied to gradually in the improvement of seawater eutrophication, and is embodying wide application prospect aspect prevention and the control coastal waters red tide with its salt resistance, resistance to soiling and high efficiency that nutritive salt is absorbed.Salicornia europaeal (Salicornia europaea L.) is that Chenopodiaceae (Chenopodiacea) salicornia europaeal (Salicornia) belongs to, and annual salt is given birth to dicotyledonous herbaceous plant.At present about the report of salicornia europaeal mainly at its salt resistance and salt tolerant Study on Mechanism (Ushakova, S.A., N.P.Kovaleva, et al. (2005) .Advances in Space Research 36 (7): 1349-1353.), (Ozawa such as Ozawa, T., M.Miura, et al. (2007) .Plant and Cell Physiology 48:S242-S242.) finds that salicornia europaeal can tolerate and accumulate heavy metal, but never see the relevant chemical action of salicornia europaeal, particularly with halobiontic interaction.
Diatom (middle Skeletonemacostatum Skeletonema castatum), Prorocentrum donghaiense (Protocentrumdonghaiense) and prorocentrum minimum (Protocentrum minimum), these three kinds of algaes are sociales that red tide takes place, in China sea area or even marine site, the whole world extensive distribution is all arranged, particularly by 2003, Skeletonema Greville occupied the first place of 19 kinds of red tide algaes that take place frequently; These two kinds of dinoflagellates not only frequently break out red tide in each sea area in recent years, also can produce various toxin, and are relevant with Xi Jia ichthyotoxin CFP with diarrhetic shellfish poison DPS.
Summary of the invention
The new purposes that the purpose of this invention is to provide salicornia europaeal (Salicornia europaea L.) and root extract thereof.
The application that the new purposes of salicornia europaeal provided by the present invention and root extract thereof is salicornia europaeal and root extract thereof in the algae growth that suppresses to cause red tide; Describedly cause that the algae of red tide is selected from following at least a: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
Another object of the present invention provides salicornia europaeal and the application of root extract in suppressing red tide thereof.
Described red tide specifically can be caused by following at least a algae: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
Above-mentioned salicornia europaeal root extract is prepared as follows:, from the salicornia europaeal root system, extract and to obtain as extractant with organic solvent, described organic solvent be selected from following any one: benzinum, chloroform, methyl alcohol and n-butanol.
A further object of the present invention provide the salicornia europaeal root exudates in the growth of the algae that suppresses to cause red tide application and suppressing application in the red tide.
Describedly cause that the algae of red tide is selected from following at least a: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
Described salicornia europaeal root exudates specifically can be salicornia europaeal and cultivate the root exudates that obtained in 30 days in the f/2 culture fluid.
Middle Skeletonemacostatum (Skeletonema castatum), Prorocentrum donghaiense (Protocentrum donghaiense) and prorocentrum minimum (Protocentrum minimum), these three kinds of algaes are sociales that red tide takes place, in China sea area or even marine site, the whole world extensive distribution is all arranged.Test shows, growth and photosynthesis ability that 4 kinds of different salicornia europaeal root extracts all can three kinds of algaes of strong inhibition, and to different algaes, its gram is given birth to action intensity difference, the centering Skeletonemacostatum, it is the strongest that the gram of benzinum phase extract is given birth to effect, its IC
50Be 1.633mg/L; To Prorocentrum donghaiense, the effect of chloroform phase extract is the strongest, its IC
50Be 13.799mg/L; To prorocentrum minimum, along with extract polarity strengthens, the gram effect of giving birth to improves, and the strongest is the methyl alcohol phase, its IC
50Be 1.138mg/L.From the inhibition effect analysis of salicornia europaeal root extract to above-mentioned three kinds of algaes, the gram living beings of biologically active are mainly low pole and nonpolar material in the salicornia europaeal root extract.Simultaneously, salicornia europaeal cultivates water filtered fluid centering Skeletonemacostatum and Prorocentrum donghaiense also shows the living effect of tangible gram, show that a water-soluble gram biomass energy secretes out by the salicornia europaeal root system, suppress the growth of red tide algae, reduced the possibility of red tide outburst to a certain extent.
Description of drawings
Fig. 1 is the growth inhibition effect of the benzinum phase extract centering Skeletonemacostatum (S.costatum) of variable concentrations among the embodiment 1, wherein: Figure 1A is the curve map of extract to the algae cell density influence, Figure 1B is the curve map of extract to the influence of frustule volume size, and Fig. 1 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 2 is the growth inhibition effect of the benzinum phase extract of variable concentrations among the embodiment 1 to Prorocentrum donghaiense (P.donghaiense), wherein: Fig. 2 A is the curve map of extract to the algae cell density influence, Fig. 2 B is the curve map of extract to the influence of frustule volume size, and Fig. 2 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 3 is the growth inhibition effect of the benzinum phase extract of variable concentrations among the embodiment 1 to prorocentrum minimum (P.minimum), wherein: Fig. 3 A is the curve map of extract to the algae cell density influence, Fig. 3 B is the curve map of extract to the influence of frustule volume size, and Fig. 3 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 4 is the growth inhibition effect of the chloroform phase extract centering Skeletonemacostatum (S.costatum) of variable concentrations among the embodiment 1, wherein: Fig. 4 A is the curve map of extract to the algae cell density influence, Fig. 4 B is the curve map of extract to the influence of frustule volume size, and Fig. 4 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 5 is the growth inhibition effect of the chloroform phase extract of variable concentrations among the embodiment 1 to Prorocentrum donghaiense (P.donghaiense), wherein: Fig. 5 A is the curve map of extract to the algae cell density influence, Fig. 5 B is the curve map of extract to the influence of frustule volume size, and Fig. 5 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 6 is the growth inhibition effect of the chloroform phase phase extract of variable concentrations among the embodiment 1 to prorocentrum minimum (P.minimum), wherein: Fig. 6 A is the curve map of extract to the algae cell density influence, Fig. 6 B is the curve map of extract to the influence of frustule volume size, and Fig. 6 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 7 is the growth inhibition effect of the n-butanol phase extract centering Skeletonemacostatum (S.costatum) of variable concentrations among the embodiment 1, wherein: Fig. 7 A is the curve map of extract to the algae cell density influence, Fig. 7 B is the curve map of extract to the influence of frustule volume size, and Fig. 7 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 8 is the growth inhibition effect of the n-butanol phase extract of variable concentrations among the embodiment 1 to Prorocentrum donghaiense (P.donghaiense), wherein: Fig. 8 A is the curve map of extract to the algae cell density influence, Fig. 8 B is the curve map of extract to the influence of frustule volume size, and Fig. 8 C is the curve map of extract to frustule chlorophyll a content influence.
Fig. 9 is the growth inhibition effect of the n-butanol phase extract of variable concentrations among the embodiment 1 to prorocentrum minimum (P.minimum), wherein: Fig. 9 A is the curve map of extract to the algae cell density influence, Fig. 9 B is the curve map of extract to the influence of frustule volume size, and Fig. 9 C is the curve map of extract to frustule chlorophyll a content influence.
Figure 10 is the growth inhibition effect of the methyl alcohol phase extract centering Skeletonemacostatum (S.costatum) of variable concentrations among the embodiment 1, wherein: Figure 10 A is the curve map of extract to the algae cell density influence, Figure 10 B is the curve map of extract to the influence of frustule volume size, and Figure 10 C is the curve map of extract to frustule chlorophyll a content influence.
Figure 11 is the growth inhibition effect of the methyl alcohol phase extract of variable concentrations among the embodiment 1 to Prorocentrum donghaiense (P.donghaiense), wherein: Figure 11 A is the curve map of extract to the algae cell density influence, Figure 11 B is the curve map of extract to the influence of frustule volume size, and Figure 11 C is the curve map of extract to frustule chlorophyll a content influence.
Figure 12 is the growth inhibition effect of the methyl alcohol phase extract of variable concentrations among the embodiment 1 to prorocentrum minimum (P.minimum), wherein: Figure 12 A is the curve map of extract to the algae cell density influence, Figure 12 B is the curve map of extract to the influence of frustule volume size, and Figure 12 C is the curve map of extract to frustule chlorophyll a content influence.
Figure 13 is the growth inhibition effect of water-phase extract centering Skeletonemacostatum, Prorocentrum donghaiense and the prorocentrum minimum of variable concentrations among the embodiment 1, wherein: Figure 13 A, B, C are the extract curve map of centering Skeletonemacostatum, Prorocentrum donghaiense and the influence of prorocentrum minimum cell density respectively, and Figure 13 D, E, F are the extract curve map of centering Skeletonemacostatum, Prorocentrum donghaiense and the influence of prorocentrum minimum cell volume size respectively.
Figure 14 is the growth inhibition effect that the salicornia europaeal of variable concentrations among the embodiment 1 is cultivated water filtered fluid centering Skeletonemacostatum and Prorocentrum donghaiense, wherein: Figure 14 A, B are the curve map of extract difference centering Skeletonemacostatum and the influence of Prorocentrum donghaiense cell density, and Figure 14 C, D are extract centering Skeletonemacostatum and the big or small curve map that influences of Prorocentrum donghaiense cell volume respectively.
Embodiment
Salicornia europaeal seedling and plant used among the embodiment obtain by following method: select full salicornia europaeal seed (Jiangsu Jinglong Ocean Industry Development Co., Ltd) and be sowed in the vermiculite germinating bed, use natural sea-water when waiting to grow rough leaf instead and cultivate.Natural sea-water is taken from the natural clean water in Xiamen west marine site (salinity 28-33psu) after acetate fiber filter membrane (0.45 μ m) filters.
To be natural sea-water filter through acetate fiber filter membrane (0.22 μ m) used seawater among the embodiment, to remove little algae and particle, adds richness with the f/2 nutrient solution, and seawater pH and salinity transfer to 8.0 ± 0.02 and 30psu respectively, cool off standby behind the autoclaving.
Used red tide algae is middle Skeletonemacostatum (S.costatum), Prorocentrum donghaiense (P.donghaiense) among the embodiment, and prorocentrum minimum (P.minimum) is provided by algae kind center, American National ocean (CCMP), and numbering is respectively 2092,1517,771.The algae kind aseptic culture of three kinds of little algaes is in f/2 culture fluid (Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms.I.Cyclotella nana Hustedt, and Detonulaconfervacea (cleve) Gran.Can J Microbiol 8:229-239) in, cultivation temperature is 20 ℃, and light intensity is 70 μ mol.m
-2.s
-1, the photoperiod is 12h.The pH of culture fluid and salinity transfer to 8.0 ± 0.02 and 30psu respectively.Regularly rock blake bottle every day 2 times, to prevent the attached wall growth of algae.
Salicornia europaeal root extract used among the embodiment is prepared according to following method:
Earlier with the fresh root system freeze drying of salicornia europaeal and pulverize, filter standby through 200 mesh sieve thin,tough silk.Adopt organic solvent that distilled water and 4 kinds have an opposed polarity (polarity from high to low: methyl alcohol (methanol)＞n-butanol (dinbutyl alcohol)＞chloroform (chloroform)＞benzinum (petroleum) extracting salicornia europaeal root powder respectively.Getting 20g root powder mixes with the 100ml benzinum, use ultrasonic extraction 1h, and be placed on extracting 24h in 20 ℃ the dark surrounds, collect extract, with with quadrat method again extracting be colourless until extract 2 times, the extract that merges 3 extracting gained, after Whatman GF/F (0.65 μ m) filters on rotary evaporator evaporated under reduced pressure, obtain black medicinal extract; When cooking extractant the salicornia europaeal powder carried out extracting with chloroform, n-butanol, first alcohol and water, same benzinum.Benzinum phase extract medicinal extract dissolves again with benzinum, obtains mother liquor, and concentration g/L represents (promptly how much restraining extract to contain in every liter of solvent), and water-phase extract directly is dissolved in the distilled water, and all the other 3 phase extracts are all used acetone solution.
Utilize the experimental data mapping among 8.0 couples of following embodiment of OriginPro; Adopt the variance analysis (ANOVA) among the SPSS17.0 to check each significance of difference of handling following experimental group and control group, and use Tukey ' s to detect (p＜0.05) the multiple ratio between each processed group.Data are mean+/-standard error (n=6) among the figure, and than (P＜0.05), same letter represents that the processed group differences is not remarkable through Tukey ' s multiple ratio, and different letter representation processed group differences are remarkable; There is significant difference between " * " expression extract-treated groups and the control group (0), " * * " expression utmost point significant difference.
1, test method
The conical flask of 100ml is adopted in experiment, includes the fresh f/2 culture fluid of 40ml.Insert three kinds of little algaes and every extract solution respectively, the initial density of algae and each extract concentrations see Table 1.The micro algae culturing liquid of f/2 culture fluid that has added equal volume is as over against according to (CK), other establish add equal volume extract solvent micro algae culturing liquid as negative contrast (0mg L
-1), benzinum is contrast with the benzinum, and water is contrast with the f/2 culture fluid then, and all the other three-phases are contrast with acetone, and the ultimate density of each solvent in culture fluid is 0.1%, the result shows that benzinum and acetone are to the not influence of growth of three kinds of algaes under this concentration.Condition of culture is same as described above.
Every group of experiment established 3 parallel group, middle Skeletonemacostatum (S.costatum) each blake bottle sampling 1ml every day, Prorocentrum donghaiense (P.donghaiense) and prorocentrum minimum (P.minimum) each blake bottle sampling in per 2 days 1 time, use Lugol ' s immobilization of reagents, and, observe the variation of microalgae cell quantity with the directly numeration under light microscope of plankton numeration plate.Reach the exponential growth later stage at control group, (Chl is content and cell size a) for chlorophyll in the sampling and measuring culture fluid.
Water sample is after Whatman GF/F filters, and filter membrane is stored in the frozen pipe, and at-20 ℃, with N, dinethylformamide (DMF) is extractant extraction 24h, and every 12h vibrates once, and extract is measured Chl a with Turner10AU Fluorometer behind suction filtration.
Middle Skeletonemacostatum (S.costatum) obtains image down at fluorescence microscope (Leica DM4500B), and use image measurement software I mage-Pro Express 6.0 measures big or small and calculates cyton (Biovolume) with following formula:
Wherein, V cell volume (μ m
3), L is cell major diameter (μ m), W is cell minor axis (μ m);
The size of two kinds of dinoflagellates and average grain volume (Mean Particle Volume (MPV)) particle collector Multisizer3 assay determination.
Table 1 salicornia europaeal root extract is given birth to the effect experiment to the gram of three kinds of red tide algaes
2, result and analysis
A, data are handled and statistical analysis
Experiment according to Chl a content in the culture fluid, is calculated algae Chl a content under individual cells and the unit volume respectively after finishing.Simultaneously, when control group algae density maximum biomass, it is made as 100%, the algae density of different experiments group is carried out standardization and done linear regression, adopt the Probit module in the SPSS17.0 software to calculate the 503nhibiting concentration (IC of extract three kinds of algaes
50).
Utilize 8.0 pairs of experimental data mappings of OriginPro; Adopt the variance analysis (ANOVA) among the SPSS17.0 to check each significance of difference of handling following experimental group and control group, and use Tukey ' s to detect (p＜0.05) the multiple ratio between each processed group.
B, salicornia europaeal root system benzinum phase extract are to the effect of three kinds of little algaes
By extract to the curve map of frustule volume size influence and extract as can be known, to the curve map (seeing Fig. 1,2 and 3) of frustule chlorophyll a content influence to the curve map of algae cell density influence, extract variable concentrations (1,5,10,15,30mg L
-1) benzinum phase extract all very obvious to the growth inhibition effect of three kinds of red tide algaes.Along with the rising of benzinum phase extract concentrations, the cell of three kinds of algaes size and Chl a content all obviously raise.The result shows that benzinum phase extract is especially the strongest to the living effect of the gram of S.costatum, when concentration surpasses 10mg L
-1The time, cell is cracking almost completely; Secondly being P.donghaiense, is P.minimum, to the IC of three kinds of algaes at last
50Be followed successively by 1.633,23.724 and 42.257mg L
-1(seeing Table 2).
C, salicornia europaeal root system chloroform phase extract are to the effect of three kinds of little algaes
The chloroform phase extract of variable concentrations all has tangible gram to give birth to effect to three kinds of algaes, and along with concentration raises, the gram effect of giving birth to strengthens, but to the modes of action of three kinds of algaes slightly different (seeing Fig. 4,5 and 6).Wherein, to S.costatum, along with chloroform phase extract concentrations raises, cell individual diminishes, and Chl a content raises in the body; On the contrary, to P.minimum, along with concentration raises, it is big that cell becomes, and Chl a content reduces; To P.donghaiense, as the lower (＜5mg L of extract concentrations
-1) time, along with concentration raises, cell individual reduces, and Chl a content also reduces, when concentration is higher, along with concentration raises, the individual increase, Chl a content raises.Chloroform phase extract is the most obvious to the living effect of the gram of S.costatum, its IC
50Be 1.889mg L
-1Effect to two kinds of dinoflagellate P.donghaiense and P.minimum does not have notable difference, its IC
50Be respectively 13.779mg L
-1With 11.936mg L
-1(seeing Table 2).
D, salicornia europaeal root system n-butanol phase extract are to the effect of three kinds of little algaes
N-butanol phase extract is given birth to effect different (seeing Fig. 7,8 and 9) to the gram of three kinds of algaes.Wherein, very obvious to the living effect of the gram of S.costatum and P.minimum, along with concentration raises, gram is given birth to act on and is strengthened, and cell individual increases, and Chl a content also raises in the body; Extract is to the IC of two kinds of algaes
50Be respectively 3.133 and 2.494mg L
-1(1-10mg L in the experimental concentration scope
-1), n-butanol phase extract has the living effect of certain gram to the growth of P.donghaiense, but not remarkable, regression analysis shows IC
50Be 30.211mg L
-1, not significantly change (seeing Table 2) of Chl a content in individual size of algae and the body in this concentration range simultaneously.
E, salicornia europaeal root system methyl alcohol phase extract are to the effect of three kinds of little algaes
Methyl alcohol phase extract has tangible gram to the growth to three kinds of algaes and gives birth to effect, and along with the rising of concentration, the gram effect of giving birth to strengthens (seeing Figure 10,11 and 12).It is remarkable than P.donghaiense that extract is given birth to effect to the gram of S.costatum and P.minimum, its above two IC
50Be respectively 1.914 and 1.138mg L
-1, and the latter has reached 14.132mg L
-1Extract is to S.costatum, and along with the rising of the living concentration of gram, Chl a content raises gradually in the body, and on the individual size of algae, except the experiment maximum concentration is 5mg L
-1The time, individual obviously change is big, and other processing do not have marked change; On the contrary, extract is to P.donghaiense, and along with concentration raises, the frustule individuality becomes greatly gradually, and except testing maximum concentration 15mg L
-1The time outside Chl a content obviously reduces in the body, other processing do not have difference; Extract is to not obviously influence (seeing Table 2) of Chl a content in the individual big or small and body of P.minimum.
F, salicornia europaeal root system water-phase extract are to the effect of three kinds of little algaes
Variable concentrations (25,50,100,200mg L
-1) that the grams of three kinds of algaes is given birth to effect is not remarkable for water-phase extract, except P.donghaiense along with extract concentrations rising cell individual become big, extract does not all have notable difference (seeing Figure 13) to Chl a content in the individual sizes of other algaes and the body.This might be too low relevant with experimental concentration, also might be that water miscible gram living beings do not exist gram to give birth to effect (seeing Table 2) to selected three kinds of algaes in the salicornia europaeal root system.
In order to verify whether effective gram living beings can be secreted in the environment, and we are experimental subjects with S.costatum and P.donghaiense, utilize the cultivation filtered water of salicornia europaeal to carry out bioassay.
Test method:
To inoculate biomass is 10,30,60g FW L
-1Salicornia europaeal in the f/2 culture fluid, cultivate 30d respectively after, remove salicornia europaeal, the filter membrane that culture fluid is crossed with autoclaving (Whatman GF/F then, 0.65 μ m and acetate fiber filter membrane, 0.22 μ m) filter, and add richness again with the f/2 nutrient solution, adjust pH and salinity to 8.0 ± 0.02 and 30psu.S.costatum and P.donghaiense are inoculated in respectively in the salicornia europaeal cultivation water filtered fluid immediately.Be incubated in contrast through two kinds of little algaes in the autoclaved f/2 culture fluid.The initial inoculation density of S.costatum and P.donghaiense is respectively 0.84 and 7 * 10
3Cells ml
-1Condition of culture is same as described above.Every group of experiment established 3 parallel group, S.costatum each blake bottle sampling 1ml every day, Lugol ' s immobilization of reagents is used in P.donghaiense each blake bottle sampling in per 3 days 1 time, and, observe the variation of microalgae cell quantity with the directly numeration under light microscope of plankton numeration plate.Reach the exponential growth later stage at control group, Chl a content and cell size in the sampling and measuring culture fluid.Method is the same.
Interpretation of result:
The result shows, in the experimental concentration scope (10,30,60g L
-1), the growth of these two kinds of algaes all is subjected to obvious suppression (seeing Figure 14), and this result with water-phase extract is different, illustrates that salicornia europaeal secretion is mainly fat-soluble material, and is consistent with our result of above-mentioned four kinds of organic facies extracts.The cultivation filtered water presents unanimity to the inhibition effect of two kinds of algaes, is lower than 30g L in concentration
-1The time, along with concentration raises, it is big that the frustule volume becomes, when excessive concentration, return to normal condition again, but different be that the gram living beings in the filtered water change the cellular morphology of S.costatum, make major diameter elongated, minor axis shortens, and does not influence the cellular morphology of P.donghaiense substantially; Examine under a microscope discovery, cultivate the P.donghaiense of filtered water after handling through salicornia europaeal, cell grow thickly and sticking group phenomenon very obvious, most frustules break.
Each phase extract of table 2 salicornia europaeal root system and cultivation water filtered fluid are given birth to the effect regression analysis to the gram of three kinds of little algaes
Claims (9)
1, the application of salicornia europaeal (Salicornia europaea L.) in the algae growth that suppresses to cause red tide; Describedly cause that the algae of red tide is selected from following at least a: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
2, the application of salicornia europaeal (Salicornia europaea L.) in suppressing red tide.
3, application according to claim 2 is characterized in that: described red tide is to be caused by following at least a algae: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
4, the application of salicornia europaeal root extract in the algae growth that suppresses to cause red tide; Describedly cause that the algae of red tide is selected from following at least a: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum; Described salicornia europaeal root extract is prepared as follows:, from the salicornia europaeal root system, extract and to obtain as extractant with organic solvent, described organic solvent be selected from following any one: benzinum, chloroform, methyl alcohol and n-butanol.
5, the application of salicornia europaeal root extract in suppressing red tide; Described salicornia europaeal root extract is prepared as follows:, from the salicornia europaeal root system, extract and to obtain as extractant with organic solvent, described organic solvent be selected from following any one: benzinum, chloroform, methyl alcohol and n-butanol.
6, application according to claim 5 is characterized in that: described red tide is caused by following at least a algae: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
7, the application of salicornia europaeal root exudates in the growth of the algae that suppresses to cause red tide; Describedly cause that the algae of red tide is selected from following at least a: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
8, the application of salicornia europaeal root exudates in suppressing red tide.
9, application according to claim 8 is characterized in that: described red tide is to be caused by following at least a algae: middle Skeletonemacostatum, Prorocentrum donghaiense and prorocentrum minimum.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942506A (en) * | 2010-05-12 | 2011-01-12 | 中国水产科学研究院东海水产研究所 | Method for rapidly detecting prorocentrum minimum by adopting LAMP (Loop-Mediated Isothermal Amplification) technology |
| CN109162148A (en) * | 2018-09-20 | 2019-01-08 | 何治伟 | A kind of preparation method of primary composite modified fire retardant papers material |
| CN111670916A (en) * | 2020-06-30 | 2020-09-18 | 北京局气网络技术有限公司 | Plant fermentation extract for preventing and treating moss in paddy field and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407361A (en) * | 2008-11-27 | 2009-04-15 | 中国科学院植物研究所 | Novel uses of Salicornia bigelovii and root extract thereof |
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2009
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942506A (en) * | 2010-05-12 | 2011-01-12 | 中国水产科学研究院东海水产研究所 | Method for rapidly detecting prorocentrum minimum by adopting LAMP (Loop-Mediated Isothermal Amplification) technology |
| CN101942506B (en) * | 2010-05-12 | 2013-02-06 | 中国水产科学研究院东海水产研究所 | Method for rapidly detecting prorocentrum minimum by adopting LAMP (Loop-Mediated Isothermal Amplification) technology |
| CN109162148A (en) * | 2018-09-20 | 2019-01-08 | 何治伟 | A kind of preparation method of primary composite modified fire retardant papers material |
| CN111670916A (en) * | 2020-06-30 | 2020-09-18 | 北京局气网络技术有限公司 | Plant fermentation extract for preventing and treating moss in paddy field and preparation method thereof |
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| Publication number | Publication date |
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| CN101647471B (en) | 2013-07-31 |
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