CN101634641A - Carbon nanofiber electrochemistry biology sensor - Google Patents
Carbon nanofiber electrochemistry biology sensor Download PDFInfo
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- CN101634641A CN101634641A CN200910070229A CN200910070229A CN101634641A CN 101634641 A CN101634641 A CN 101634641A CN 200910070229 A CN200910070229 A CN 200910070229A CN 200910070229 A CN200910070229 A CN 200910070229A CN 101634641 A CN101634641 A CN 101634641A
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Abstract
The invention relates to a preparation method for a carbon nanofiber electrochemistry biology sensor, which is as follows: firstly, processing carbon nanofiber CNF and than preparing CNF and an enzyme modified electrode. In the condition of the presence of NAD<+>, the ADH fixed in the modified electrode can catalyze alcohol and simultaneously NAD<+> is reduced to NADH. As CNF has very good electrochemistry oxidation performance on the NADH, NADH generated by detection is used for indirectly detecting alcohol content in the sample. Hereby, a field rapid detection analyzer for human body blood alcohol content is manufactured and used for instantly and rapidly testing the blood alcohol content of a driver on traveling crane field, thus instantly and rapidly judging whether the driver drinks on the field, thus effectively preventing a drinker from driving a car and avoiding the occurrence of traffic accidents.
Description
Technical field
The invention belongs to the ethanol electrochemica biological sensor that utilizes the preparation of carbon nano-fiber (CNF) and alcohol dehydrogenase and to the detection of alcohol content in the blood of human body, particularly a kind of carbon nanofiber electrochemistry biology sensor.
Background technology
Along with expanding economy, global automobile quantity is increased sharply, and the traffic accident incidence also soars, and according to the numeral that European Union's white paper provides, full Europe is respectively 4.1 ten thousand and 1,700,000 because of death and the number of injured people that traffic accident causes every year, and direct economic loss is 160,000,000,000 Euros.And the numeral that the World Health Organization (WHO) announces is more startling, and the whole world has 1,300,000 people to die from traffic hazard every year, and more than 1,200 ten thousand people are injured, and economic loss is equivalent to annual Milan city and is erased from the earth up to 5,180 hundred million Euros.In China, because of more than 10 ten thousand people are all arranged in every year of traffic hazard death, far above the death toll of time world's size war in recent years, rough calculation just had a Chinese to be captured life by automobile in average per five minutes.Data presentation, though that the Chinese automobile recoverable amount only accounts for is global 2%, the death by accident number accounts for global about 15%.According to road traffic control authority office statistics: occur in the road traffic accident in all parts of the country every year, it is because of due to driving when intoxicated that 37% accident is arranged.Traffic safety in the serious threat of driving when intoxicated, and has become " a big killer " in the traffic hazard.The easy accidents caused reason of driving when intoxicated is the principal ingredient ethanol of wine, because ethanol can have very big influence to people's nervous system.The foundation of judging when the driver drives 80% is from visual determination, and under the state of, Consciousness clear in vision, traffic hazard just is difficult for taking place, and can make people's eye-blurred unclear but drink.And because the effect of alcohol, people's balanced capacity, the order of accuarcy of action all can reduce greatly, thereby action is morphed.In addition, drinking also to make reaction capacity reduction, the locomitivity imbalance of brain to accident, and because of the alcohol anesthetic action, people's hand, pin sense of touch reduce more at ordinary times, often can't normally control throttle, brake and bearing circle.At this moment drive very easily to cause accident as accident.Many countries have all formulated the relation of ethanol in blood content and traffic accident responsibility identification, have set up the multiple method that can detect ethanol in blood content.As instrument detecting alcohol components such as GC/MS, though these methods can be quantitative, exist the purchase instrument and cost an arm and a leg, detect the cost height, need special training, can't be in scarce limits such as spot fast detecting.Expiration formula alcohol determining instrument can use at the scene, but price is also very expensive, detects the cost height, and unhygienic, cross infection disease takes place easily and be subjected to that easily artificial false expiration influences etc.The Ministry of Public Security issued " about the traffic offence behavior of driving when intoxicated being carried out the emergency notice of sole rectification " 2007 on February 19,, don, contain the generation of particularly serious road traffic accident, guarantee the people's safety, be an arduousness and great task, it is whole people's engineering, it is the people's war of a new period, the traffic police is the vanguard of this war, but need technical force's reinforcement, the method and apparatus of being badly in need of the quick alcohol check in a kind of scene satisfies the needs of realistic development.
Nicotinamide adenine dinucleotide reduced (NADH) and its oxidised form (NAD
+) be the coenzyme of a kind of transmission proton (more accurate is hydrogen ion), it appears in a lot of metabolic responses of cell, is the co-factor that participates in 300 multienzymatic reactions.Owing to NADH importance as co-factor in enzyme reaction, so people expect to utilize the electrochemical oxidation to NADH to prepare biology sensor.But,, reduce overpotential and electrode surface and pollute so many materials are used to catalyzing N ADH electrochemical oxidation owing to very high overpotential is arranged and can produce pollution to electrode surface at general electrode surface oxidation NADH.In recent years, nano material is one of current hot research field.Carbon nano-fiber (CNF) is a kind of non-hollow carbon fiber of nano-grade size.Because its particular structure and character, it has potential widely application at aspects such as catalyst support, probe tip and fuel cells.After handling through nitric acid oxidation, CNF can produce a lot of oxy radicals and its agent structure can be not destroyed.The oxy radical of these generations can promote the electro-catalysis to nicotinamide adenine dinucleotide reduced (NADH).Because material with carbon element dispersed very poor in most of solvents, and the CNF of acidification has dissolubility preferably in water, help modification and stability at electrode surface.The present invention utilizes CNF that NADH has been represented highly sensitive electrocatalysis characteristic.In conjunction with alcohol dehydrogenase (ADH), prepare a kind of high-sensitive alcohol electrochemica biological sensor then.
Summary of the invention
Technical matters to be solved by this invention is: a kind of carbon nanofiber electrochemistry biology sensor is provided.It is to the detection of alcohol content in the blood.There is NAD
+Condition under, the ADH that is fixed in the modified electrode can catalysis ethanol, simultaneously NAD
+Be reduced to NADH.Because CNF has good electrochemistry oxidation performance to NADH, so we utilize and detect the NADH that generates and come ethanol content in the test sample indirectly.
Technical scheme of the present invention is:
A kind of preparation method of carbon nanofiber electrochemistry biology sensor:
Its preparation process is as follows:
(1). the processing of carbon nano-fiber CNF:
1mg-100mg carbon nano-fiber CNF is distributed to 10-60ml, and in the 10%-60% salpeter solution, potpourri was 30 ℃ to 150 ℃ heating 1 hour to 48 hours; Product obtains the CNF of carboxylation after through centrifugal and washing; Obtain the carbon nano-fiber CNF aqueous solution of 1-10mg/ml by carbon nano-fiber CNF ultrasonic dispersion in water;
(2) preparation of .CNF and enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water; After drying up with nitrogen, 1-20 μ L, the 0.2M of 0.5-10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5-9 drips to glass-carbon electrode, dry 30min in exsiccator; And then the carbon nano-fiber CNF solution that drips 1-20 μ L drips on the glass-carbon electrode and the dry carbon nanofiber electrochemistry biology sensor that gets in exsiccator.
Effect of the present invention is:
This a kind of carbon nanofiber electrochemistry biology sensor, it can be to the detection of alcohol content in the blood of human body.There is NAD
+Condition under, the ADH that is fixed in the modified electrode can catalysis ethanol, simultaneously NAD
+Be reduced to NADH.Because CNF has good electrochemistry oxidation performance to NADH, so we utilize and detect the NADH that generates and come ethanol content in the test sample indirectly.
Can make human body alcohol content field quick detection analyzer with this, whether the instant test of blood ethanol content is fast carried out to the driver in the scene that can be used for driving a vehicle, drink thereby can judge the driver at the scene immediately apace.Thereby can prevent the people that drinks to drive the generation that avoids traffic accident effectively.
Embodiment
The invention provides a kind of carbon nanofiber electrochemistry biology sensor.
Preparation process is as follows:
1.CNF processing:
1mg-100mg CNF is distributed to 10-60ml, and in the 10%-60% salpeter solution, potpourri heats 1h to 48h at 30 ℃ to 150 ℃.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 1-10mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 1-20 μ L, the 0.2M of 0.5-10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5-9 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 1-20 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH5-9 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.At BAC is under the 10-425mM, and response current detects and is limited to 3 μ M with the linear variation of alcohol concentration, and relative standard deviation is 5.1%.
Embodiment 1:
1.CNF processing:
1mg mg CNF is distributed to 10ml, and in 10% salpeter solution, potpourri is at 50 ℃ of heating 1h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 1mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 1 μ L, the 0.2M of 0.5mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 1 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol
Containing 5mMNAD
+The buffer solution of pH5 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 10-30mM.
Embodiment 2:
1.CNF processing:
2mg CNF is distributed to 30ml, and in 10% salpeter solution, potpourri is at 50 ℃ of heating 7h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 3mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 1 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 1 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 10-50mM.
Embodiment 3:
1.CNF processing:
10mg CNF is distributed to 20ml, and in 10% salpeter solution, potpourri is at 80 ℃ of heating 12h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 3mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 10 μ L, the 0.2M of 0.5mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 10 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH5 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 40-80mM.
Embodiment 4:
1.CNF processing:
2mg CNF is distributed to 30ml, and in 10% salpeter solution, potpourri is at 50 ℃ of heating 7h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 3mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 5 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 5 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 20-100mM.
Embodiment 5:
1.CNF processing:
10mg CNF is distributed to 40ml, and in 10% salpeter solution, potpourri is at 80 ℃ of heating 2h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 10mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 2 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 2 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 25-150mM.
Embodiment 6:
1.CNF processing:
20mg CNF is distributed to 60ml, and in 10% salpeter solution, potpourri is at 120 ℃ of heating 48h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 20mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 4 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 4 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 20-350mM.
Embodiment 7:
1.CNF processing:
20mg CNF is distributed to 40ml, and in 10% salpeter solution, potpourri is at 150 ℃ of heating 48h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 8mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 15 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 15 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 80-400mM.
Embodiment 8:
1.CNF processing:
60mg CNF is distributed to 40ml, and in 10% salpeter solution, potpourri is at 50 ℃ of heating 48h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 10mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 20 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 20 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol ":
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 20-280mM.
Embodiment 9:
1.CNF processing:
100mg CNF is distributed to 40ml, and in 10% salpeter solution, potpourri is at 80 ℃ of heating 48h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 9mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 15 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 15 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 20-380mM.
Embodiment 10:
1.CNF processing:
60mg CNF is distributed to 20ml, and in 10% salpeter solution, potpourri is at 150 ℃ of heating 1h.Product obtains the CNF of carboxylation after through centrifugal and washing.Obtain the CNF aqueous solution of 8mg/ml by CNF ultrasonic dispersion in water.
2.CNF and the preparation of enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water.After drying up with nitrogen, 10 μ L, the 0.2M of 10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5 drips to glass-carbon electrode, dry 30min in exsiccator.And then the CNF solution that drips 10 μ L drips on the glass-carbon electrode and the dry ethanol biology sensor that gets in exsiccator.
3.CNF and enzyme modified electrode is to the Electrochemical Detection of alcohol:
Containing 5mMNAD
+The buffer solution of pH7 in, with electrochemical analyser CHI660 (CH company, the U.S.) (working electrode is a modified glassy carbon with three electrode work systems, to electrode is platinum filament, contrast electrode is saturated calomel electrode SCE), under the 0.1V current potential, carry out the detection that electric current changes with blood alcohol concentration.Detecting the range of linearity is 20-210mM.
Claims (1)
1, a kind of preparation method of carbon nanofiber electrochemistry biology sensor:
Its preparation process is as follows:
(1). the processing of carbon nano-fiber CNF:
1mg-100mg carbon nano-fiber CNF is distributed to 10-60ml, and in the 10%-60% salpeter solution, potpourri was 30 ℃ to 150 ℃ heating 1 hour to 48 hours; Product obtains the CNF of carboxylation after through centrifugal and washing; Obtain the carbon nano-fiber CNF aqueous solution of 1-10mg/ml by carbon nano-fiber CNF ultrasonic dispersion in water;
(2) preparation of .CNF and enzyme modified electrode:
Glass-carbon electrode is used the aluminium oxide powder polishing of 1,0.3 and 0.05 μ m successively, then with the flushing of secondary water; After drying up with nitrogen, 1-20 μ L, the 0.2M of 0.5-10mg/ml alcohol dehydrogenase, the phosphate buffer solution of pH 5-9 drips to glass-carbon electrode, dry 30min in exsiccator; And then the carbon nano-fiber CNF solution that drips 1-20 μ L drips on the glass-carbon electrode and the dry carbon nanofiber electrochemistry biology sensor that gets in exsiccator.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022056978A1 (en) * | 2020-09-17 | 2022-03-24 | 南京工业大学 | Method for preparing nadh and ethanol biosensing chip |
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| CN1737560A (en) * | 2005-09-02 | 2006-02-22 | 天津大学 | Static electricity spinning prepared fixed enzymic electrode and method thereof |
| CN1912200A (en) * | 2006-08-15 | 2007-02-14 | 浙江大学 | Nano-fibre of carbon nanotube and its method of preparation and oxidation reduction fix |
| CN101109098A (en) * | 2007-06-28 | 2008-01-23 | 上海交通大学 | Method for implanting carbon nano-tube/carbon nano fabric in metal electrode surface layer |
| US20080257015A1 (en) * | 2006-11-28 | 2008-10-23 | Charles Martin Lukehart | Graphitic-carbon-nanofiber/polymer brushes as gas sensors |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1737560A (en) * | 2005-09-02 | 2006-02-22 | 天津大学 | Static electricity spinning prepared fixed enzymic electrode and method thereof |
| CN1912200A (en) * | 2006-08-15 | 2007-02-14 | 浙江大学 | Nano-fibre of carbon nanotube and its method of preparation and oxidation reduction fix |
| US20080257015A1 (en) * | 2006-11-28 | 2008-10-23 | Charles Martin Lukehart | Graphitic-carbon-nanofiber/polymer brushes as gas sensors |
| CN101109098A (en) * | 2007-06-28 | 2008-01-23 | 上海交通大学 | Method for implanting carbon nano-tube/carbon nano fabric in metal electrode surface layer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022056978A1 (en) * | 2020-09-17 | 2022-03-24 | 南京工业大学 | Method for preparing nadh and ethanol biosensing chip |
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Effective date of abandoning: 20100127 |
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| C20 | Patent right or utility model deemed to be abandoned or is abandoned |