CN101628132A - Bionic dermis and preparation method and application thereof - Google Patents
Bionic dermis and preparation method and application thereof Download PDFInfo
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- CN101628132A CN101628132A CN200810040668A CN200810040668A CN101628132A CN 101628132 A CN101628132 A CN 101628132A CN 200810040668 A CN200810040668 A CN 200810040668A CN 200810040668 A CN200810040668 A CN 200810040668A CN 101628132 A CN101628132 A CN 101628132A
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Abstract
The invention discloses a bionic dermis. An implant is a three-dimensional braided fabric of biocompatible fiber, and the fiber is adsorbed, absorbed or enwrapped with an extracellular matrix component substance of normal skins and/or dermis tissues which is advantageous for repairing skins or dermises. The invention also discloses a preparation method and application of the bionic dermis.
Description
Technical field
The present invention relates to the medical surgery patching material, relate in particular to a kind of bionic dermis and its production and use.
Background technology
Skin covering directly contacts with external environment in the whole body surface, is anatomy and physiological critical boundaries organ.Skin is made up of epidermis and corium, borrows subcutaneous tissue to link to each other with deep fascia, aponeurosis (aponeuroses) or the periosteum in deep.Skin originates from ectoderm and mesoderm; complex structure and eggcase have important barrier action and protective effect, can prevent that extraneous stimulation from damaging intravital tissue; can stop foreign body and microorganism intrusion, and can stop body fluid to blend the absorption of material to external world outward.
Skin is the barrier that contacts with the external world as the organ of human body maximum, and when causing skin injury owing to factors such as extraneous damage or diseases, its harm can be extremely slight, also can be fatal.
At present, skin injury reparation approach commonly used has: from body skin-grafting, allograft of skin, xenogenesis skin-grafting, but defectives such as supplying to distinguish deficiency, immunologic rejection and spreads disease are arranged.
Also have the substitute of some skins to make at present, but lack normal skin corium mechanical characteristic with Chinese herbal medicine.
Therefore, this area presses for provides a kind of ideal Graftskin, to satisfy clinical demand.
Summary of the invention
The present invention aims to provide a kind of bionic dermis.
Second purpose of the present invention provides the preparation method of described bionic dermis.
The 3rd purpose of the present invention provides the purposes of described bionic dermis.
In a first aspect of the present invention, a kind of bionic dermis is provided, described bionic dermis is the three-dimensional braid of biocompatible fibres, and described fiber is adsorbed with, is absorbed with or be enclosed with the normal skin that is beneficial to skin or dermis restoration and/or the protein matter and/or the non-protein matter of dermal tissue extracellular matrix components.
In another preference, described three-dimensional braid is inequality at the braiding structure of x, y and z three-dimensional.By special three dimensional knitting method the dermal tissue structure is carried out highly bionically, have the characteristic of skin soft tissue, as anisotropy, viscoelasticity etc.
In another preference, described three-dimensional braid is to be formed by 3,4,5,6,7,8,9 or 10 biocompatible fibres braidings.
In another preference, the protein matter that contains normal skin and/or dermal tissue extracellular matrix components alternately occurs on the silvalin of being twisted by described biocompatible fibres with non-protein matter.
In another preference, the diameter of described biocompatible fibres is the 8-100 micron.
In another preference, the aperture of described fabric is 30-2000 μ m; Preferably, being 50-1500 μ m, more preferably is 70-1000 μ m.
In another preference, the thickness of described fabric is 0.1-3.2mm; Preferably, being 0.2-2.0mm, more preferably is 0.4-1.6mm.
In another preference, describedly be beneficial to the normal skin of skin or dermis restoration and/or the protein matter of dermal tissue extracellular matrix components comprises: adherent protein molecular takes place in somatomedin, proteoglycan, glycoprotein, promotion cell and described fiber, and at present may still undiscovered somatomedin and reduce cicatrix and produce the factor.
In another preference, describedly promote cell and described fiber that adherent protein molecular takes place to comprise normal skin and/or dermal tissue extracellular matrix components that they are: Fibronectin (FN), collagen, non-collagen sugar albumen, elastin laminin, Dan Baijutang and at present may still undiscovered short viscoelastic elements.
In another preference, described non-protein matter is selected from Polyethylene Glycol, aminoglycan, poly-l-lysine-g-Polyethylene Glycol.
In another preference, described fiber is the fiber that absorbable biocompatible materials constitutes.
In a second aspect of the present invention, a kind of preparation method as above-mentioned bionic dermis is provided, it comprises step:
(a) will be adsorbed with, be absorbed with or be enclosed with the biocompatible fibres of the protein matter of the normal skin that is beneficial to skin or dermis restoration and/or dermal tissue extracellular matrix components and/or non-protein matter or silvalin carries out 3 D multi-directional (3-10 to) method braiding and obtains as above-mentioned bionic dermis; Or
(1) biocompatible fibres is carried out be immersed in after the braiding of 3 D multi-directional (3-10 to) method in the protein matter that contains normal skin and/or dermal tissue extracellular matrix components and/or the non-protein matter and obtain as above-mentioned bionic dermis.
In another preference, described three-dimensional biocompatible fibres has 3,4,5,6,7,8,9 or 10.
In another preference, described step (a) is preceding to comprise step (a '): will be adsorbed with, be absorbed with or be enclosed with the normal skin that is beneficial to skin or dermis restoration and/or the protein matter of dermal tissue extracellular matrix components and/or the biocompatible fibres of non-protein matter and twist into silvalin (promptly carrying out the braiding of plait sample).
Through the braiding of plait sample, the fiber and the generation of PEG fiber that then contain different protein ingredients interweave, tension then, make the gap less than 30 microns, greater than cell dia, then cell can not enter, and only may contact with the PEG composition with the surface protein composition after the braiding, forms the directive function of bridge pier like this.
In another preference, described step (a) is: will be adsorbed with, be absorbed with or be enclosed with the biocompatible fibres of the protein matter of extracellular matrix components of the normal skin that is beneficial to skin or dermis restoration and/or dermal tissue and/or non-protein matter or silvalin and carry out 3 D multi-directional (3-10 to) method braiding, and add angle interlocking multilamellar (at least three layers) tissue weave again and weave the bionic dermis that obtains as above-mentioned.
In another preference, have different chemical groups on the biocompatible fibres described in the step (1), described chemical group comprises hydrophobic, hydrophilic, positively charged or electronegative.
In another preference, described hydrophobicity chemical group is selected from: mercapto, aromatic ether; Described hydrophilic chemical group is selected from: amino, hydroxyl, carboxyl, sulfonic group, ether; Described positively charged chemical group is selected from: chitosan, amino, guanidine radicals, imidazole radicals; Described electronegative chemical group is selected from: carboxyl.
In another preference, the preceding step (1 ') that comprises of described step (1): biocompatible fibres is twisted into silvalin (promptly carrying out the braiding of plait sample).
In another preference, described step (1) is: biocompatible fibres is carried out the braiding of 3 D multi-directional (3-10 to) method, add in the protein matter that is immersed in normal skin and/or dermal tissue extracellular matrix components at least after angle interlocking multilamellar (three layers) tissue weave weaves and/or the non-protein matter again and obtain as above-mentioned bionic dermis.
In a third aspect of the present invention, provide a kind of as above-mentioned application of bionic dermis in the graft of preparation reparation damaged skin or corium.
In another preference, described graft can reduce cicatrization.
In view of the above, the invention provides a kind of ideal Graftskin, can satisfy clinical demand.
Description of drawings
Fig. 1 is that the bridge pier like cell sticks sketch map;
Wherein, A has shown the three-dimensional sight of single fiber, and B has shown bundle fiber plane sight; Dark color is a bridge pier among A, B two figure, is enclosed with FN, and light color is for being enclosed with PEG.
Fig. 2 is that the bridge pier like cell sticks design drawing;
Wherein, A has shown sight under low power (20 times) magnifier; B has shown sight under high power (60 times) magnifier; C has shown the design size of corresponding construction, and each unit is length and width, the spacing of the different bridge piers of design among the C.
Fig. 3 is the braiding flow chart;
Wherein the bottom right is the fiber of finishing, dark showed cell attachment point.
Fig. 4 has shown weave effect figure.
Fig. 5 is the weave effect figure that Electronic Speculum shows.
The specific embodiment
The inventor has developed a kind of bionic dermis through extensive and deep research, and it is formed through special 3 D weaving by micron-sized biocompatible fibres.Be adsorbed with, be absorbed with on the biocompatible fibres of bionic dermis provided by the invention or be enclosed with the protein matter that is beneficial to skin or dermis restoration, after transplanting, can form the cell adhesion state of bridge pier sample, have the synulotic effect of minimizing.
Particularly, the present invention is at first soaked into different monfil (micron order biocompatible fibres) in the protein molecular solution of heterogeneity earlier, and taking-up is dried, and carries out the braiding of plait sample; Adopt then 3 D multi-directional 5 to, or 6 to, or 7 to, or weave to method greater than 7, add angle interlocking multilamellar (at least three layers) tissue weave again and weave; Carry out mechanics at last and stretch to detect, can use after meeting the skin self character.
Perhaps, the present invention earlier carries out different chemistry, physical treatment with fiber (micron order biocompatible fibres), they is become have different hydrophobicitys, hydrophilic, or increase different chemical groups or make different fibers with different positive and negative charges; Carry out 3 D multi-directional 5 step (6,7, more than) method then, add angle interlocking multilamellar (at least three layers) tissue weave and weave, detect after meeting skin properties standby through mechanics; Last according to different hydrophilic, hydrophobicity, or different chemical group, or positive and negative charge character, infiltration contain normal skin and/or dermal tissue extracellular matrix components protein molecular solution in, carry out the molecule self assembly, the protein molecular of this heterogeneity is the different protein ingredients that had in the dermal tissue, comprises various somatomedin etc., takes out and dries the back use.
Definition
As used herein, " fiber " or " biocompatible fibres " all represents a kind of material, and its used material is biological absorbable biocompatible materials, and the external diameter of cross section is 60-300 μ m, preferably is 20-100 μ m, more preferably is 4-13 μ m; Described biological absorbable biocompatible materials is selected from:
(i) degradability synthesized polymer material, for example poly-'alpha '-hydroxy acids (as polylactic acid PLA, polyglycolic acid PGA, poly butyric PHB etc.), poly-anhydride (polyanhydrides), poly-phosphazo (polyphosphazenes), polyamino acid (polyamino acid), false polyamino acid (pesudo-polyamino acid), poe (polyorthoesters), polyester urethane (polyesterurethane), Merlon (polycarbonate), Polyethylene Glycol, poly-P-Dioxane ketone (polydioxanone) etc.;
(ii) natural degradable material, for example collagen (collagen), gelatin (gelatin), ammonia polyose of candy (glycosaminoglycan, GAGs), chitosan (chitosan), chitin (chitin), alginate, calcium alginate gel etc.; Various acellular matrixes;
(iii) syringeability material is as Pluronic (a kind of commercially available poly-epoxy second propylene commodity), calcium alginate gel etc.;
The composite of (iv) composite of the mixture of above-mentioned material or composite, especially macromolecular material and natural material, and solid material and syringeability material.
As used herein, " three-dimensional braid " is inequality at the braiding structure of x, y and z three-dimensional.It is not the simple superposition of plane knitting product, but makes three-dimensional produce the woollen yarn knitting mode of different structure by the method for stereo weaving.Can be described as is the fabric with soft tissue biomechanics characteristic of " anisotropy ".
As used herein, " molecule self assembly " is meant that getting normal skin tissue carries out homogenate, and composition is wherein carried out mass spectral analysis, and protein molecular is identified, instructs the molecule grafting of the support of my material, corresponding proteins molecule in the grafting according to the result who analyzes.
Bionic dermis
As used herein, " bionic dermis " is a kind of graft that can be used for skin or dermis restoration.It is by the fabric that micron order biocompatible fibres woollen yarn knitting forms, and used fiber is at least 3, preferably is the 3-7 root, more preferably is 5; Must be adsorbed with, be absorbed with or be enclosed with the normal skin that is beneficial to skin or dermis restoration and/or the protein matter of dermal tissue extracellular matrix components on the used fiber, with non-protein matter, and two kinds of metabolies occur, be bridge pier sample (seeing accompanying drawing 1), bridge pier length is 1-40 μ m, is preferably 2-20 μ m; Width is 0.5-10 μ m,, preferably be 1.5-5 μ m; Spacing between bridge pier is 1-20 μ m, preferably is 2-10 μ m.More preferably, said bridge pier size and following combination of spacing: (1) 2 * 1.5, spacing 2 μ m; (2) 8 * 2, spacing 4 μ m; (3) 16 * 3, spacing 6 μ m; (4) 2 * 2, spacing 4 μ m; (5) 8 * 3, spacing 6 μ m; (6) 16 * 2, spacing 4 μ m; (7) 20 * 3, spacing 2 μ m; (8) 16 * 5, spacing 8 μ m; Or its combination.
About the influence of power, the tension force that the fabric itself that it is good that this power refers to braiding is had, the elastic modelling quantity that this tension force altitude simulation normal skin is had, available Young ' s elastic modelling quantity represent that this mechanics parameter can be selected between 3kPa-1000kPa.
Absorption, absorb or be wrapped in the normal skin that is beneficial to skin or dermis restoration on the fiber and/or the protein matter of dermal tissue extracellular matrix components comprises: adherent protein molecular and may still undiscovered at present somatomedin and reduce the cicatrization factor takes place in somatomedin, proteoglycan, glycoprotein, promotion cell and described fiber.
Absorption, absorb or be wrapped in and do not have the effect of sticking with cell on the fiber, as with AC protein separately material be selected from Polyethylene Glycol, poly-l-lysine-Polyethylene Glycol (PLL-g-PEG).
Preparation method
Bionic dermis provided by the invention can be made by several different methods, a kind of method comprises step: earlier different single fibers is immersed in the solution that contains different protein molecular compositions, described protein molecular composition is selected from normal skin and/or dermal tissue extracellular matrix components: as Fibronectin, collagen, vitronectin, hyaluronic acid, non-collagen sugar albumen, elastin laminin, syndecan, aminoglycan, glycoprotein, and at present may still undiscovered somatomedin and reduce the cicatrization factor, Polyethylene Glycol carries out the braiding of plait sample after taking-up is dried; Carry out then 3 D multi-directional 5 to or 6 to 7 to or weave to method greater than 7, add angle interlocking multilamellar (at least three layers) tissue weave again and weave and obtain bionic dermis.
Another kind of method of the present invention comprises step: earlier different single fibers is carried out different chemistry and/or physical treatment respectively, make different fibers have different hydrophobicitys, hydrophilic, or make different fibers increase different chemical groups, or make different fibers have different positive and negative charges; Carry out then 3 D multi-directional 5 to or 6 to 7 to or weave to method greater than 7, add angle interlocking multilamellar (at least three layers) tissue weave again; Last according to hydrophobicitys different on the fiber, hydrophilic, or different chemical groups, or different positive and negative charge character, be immersed in the protein molecular solution that contains normal skin and/or dermal tissue extracellular matrix components, carry out the molecule self assembly, obtain bionic dermis provided by the invention.
Purposes
Bionic dermis provided by the invention can be used to prepare the graft of repairing damaged skin; Described graft can reduce cicatrization.
According to the wound surface size, select the corium template of different sizes, after this corium template sterilization, wound surface is local to be transplanted after suitably handling, and chooses the sword pachydermia then and transplants thereon, then pressure dressing.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can combination in any.All features that this case description is disclosed can with any composition forms and usefulness, each feature that is disclosed in the description can anyly provide the alternative characteristics of identical, impartial or similar purpose to replace.Therefore removing has special instruction, and the feature that is disclosed only is the general example of equalization or similar features.
Major advantage of the present invention is:
1, bionic dermis provided by the invention has the mechanical characteristic of normal skin corium, and it has anisotropy and yielding and have viscoelasticity, creep, and the characteristics of stress relaxation, this is not available in the tissue engineering material support that carries out of present nonwoven braiding;
2, bionic dermis provided by the invention has stable characteristics, and this also is to adopt non-woven technology not available in the present organization engineering skin support;
3, bionic dermis preparation method provided by the invention is simple and easy to do;
4, bionic dermis provided by the invention helps large-scale industrial production;
5, bionic dermis provided by the invention is with low cost.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.Unless otherwise indicated, otherwise all percentage ratio and umber by weight.
Unless otherwise defined, the same meaning that employed all specialties and scientific words and one skilled in the art are familiar with in the literary composition.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The usefulness that preferable implementation method described in the literary composition and material only present a demonstration.
Embodiment 1
Preparation bionic dermis I
Normal structure skin is taken off cell handle, make homogenate then.Comprised various my above-mentioned various cell growth factor in this normal structure, proteoglycan, promotes cell and the adherent protein molecular of fiber and at present may still undiscovered somatomedin and reduce the cicatrization factor at glycoprotein.
Different fibers are carried out 3 D multi-directional (4,5,6,7 to) braiding earlier, immerse in the homogenate of above-mentioned preparation again, room temperature after 30 minutes, is taken out, and dries.Obtain described corium template.Area can be fixed according to hindering the face area.
Then the corium template is implanted wound surface, surperficial sword pachydermia is transplanted, pressure dressing.
Embodiment 2
Preparation bionic dermis II
3,4,5,6,7,8,9,10,11 of different fibers are immersed respectively in the normal skin and/or dermal tissue extracellular matrix components homogenate of variable concentrations, and immerse among PEG or the PLL-g-PEG (1mg/ml), take out then, dry, carry out various combination, three, or five or seven, comprise PEG or PLL-g-PEG in every group of combination, plait the hair into fibre bundle then, again the fibre bundle of making is carried out 3 D multi-directional five to, seven to braiding, makes corium template (being exactly bionic dermis II).Area can be fixed according to hindering the face area.
Then the corium template is implanted wound surface, surface transplantation is from body sword pachydermia, pressure dressing.
The mechanics testing result, according to the scar tissue different times, hypertrophic cicatrix is early stage, and in mid-term, the elastic modelling quantity of late period and normal skin prepares corresponding corium template, carries out mechanics then and detects, and whether reaches above standard.It is qualified to be reached for, and experimentizes according to different elastic modelling quantity then.
Structural parameters, thickness are the thickness 1-1.5mm of corium, and the aperture is 30-100nm, and the number of plies is that the diameter according to thickness and fiber decides.
Embodiment 3
The zoopery of bionic dermis
In this embodiment, utilize zoopery to test, method of testing is as follows: and the cleaning Du Lao gram pig of selection some (available from: Shanghai Slac Experimental Animal Co., Ltd.), lost hair or feathers back 24 hours in the back, cut a certain size holostrome skin at the pig back with scalpel, the anti-sword pachydermia of getting on the drum-type dermatome is as from the body skin.Wound surface is divided into four groups at random:
Organize 1 wound surface and be the experiment wound surface, after the hemostasis, transplant earlier the bionic dermis of preparation among the embodiment 1, autologous transplanting skin thereon then, pressure dressing;
Organize 2 wound surface and be the experiment wound surface, after the hemostasis, transplant earlier the bionic dermis of preparation among the embodiment 2, autologous transplanting skin thereon then, pressure dressing;
Organize 3 wound surface and be the contrast wound surface, after the hemostasis, it is thick in the body skin to transplant sword, pressure dressing;
Organize 4 wound surface for the contrast wound surface, after the hemostasis, this holostrome skin is transplanted on the wound surface pressure dressing again.
The above only is preferred embodiment of the present invention, be not in order to limit essence technology contents scope of the present invention, essence technology contents of the present invention is broadly to be defined in the claim scope of application, any technology entity or method that other people finish, if it is defined identical with the claim scope of application, also or a kind of change of equivalence, all will be regarded as being covered by among this claim scope.
Claims (10)
1. bionic dermis, it is characterized in that, described bionic dermis is the three-dimensional braid of biocompatible fibres, and described fiber is adsorbed with, is absorbed with or be enclosed with the normal skin that is beneficial to skin or dermis restoration and/or the protein matter and/or the non-protein matter of dermal tissue extracellular matrix components.
2. bionic dermis as claimed in claim 1 is characterized in that, described three-dimensional braid is to be formed by 3,4,5,6,7,8,9 or 10 biocompatible fibres braidings.
3. bionic dermis as claimed in claim 1 is characterized in that, the protein matter of normal skin and/or dermal tissue extracellular matrix components and/or non-protein matter alternately occur on the silvalin of being twisted by described biocompatible fibres.
4. the preparation method of a bionic dermis as claimed in claim 1 is characterized in that, it comprises step:
(a) will be adsorbed with, be absorbed with or be enclosed with the biocompatible fibres of the protein matter of the normal skin that is beneficial to skin or dermis restoration and/or dermal tissue extracellular matrix components and/or non-protein matter or silvalin carries out 3 D multi-directional (3-10 to) method braiding and obtains bionic dermis as claimed in claim 1; Or
(1) biocompatible fibres is carried out be immersed in after the braiding of 3 D multi-directional (3-10 to) method in the protein matter that contains normal skin and/or dermal tissue extracellular matrix components and/or the non-protein matter and obtain bionic dermis as claimed in claim 1.
5. preparation method as claimed in claim 4, it is characterized in that, in the preceding step (a ') that comprises of step (a): will be adsorbed with, be absorbed with or be enclosed with the normal skin that is beneficial to skin or dermis restoration and/or the protein matter of dermal tissue extracellular matrix components and/or the biocompatible fibres of non-protein matter and twist into silvalin.
6. preparation method as claimed in claim 4, it is characterized in that, described step (a) is: will be adsorbed with, be absorbed with or be enclosed with the biocompatible fibres of the protein matter of the normal skin that is beneficial to skin or dermis restoration and/or dermal tissue extracellular matrix components and/or non-protein matter or silvalin and carry out 3 D multi-directional (3-10 to) method braiding, and add angle interlocking multilamellar (at least three layers) tissue weave again and weave and obtain bionic dermis as claimed in claim 1.
7. preparation method as claimed in claim 4 is characterized in that, has different chemical groups on the biocompatible fibres described in the step (1), and described chemical group comprises hydrophobic, hydrophilic, positively charged or electronegative.
8. preparation method as claimed in claim 4 is characterized in that, in the preceding step (1 ') that comprises of step (1): biocompatible fibres is twisted into silvalin.
9. preparation method as claimed in claim 4, it is characterized in that, described step (1) is: biocompatible fibres is carried out the braiding of 3 D multi-directional (3-10 to) method, add to be immersed in after angle interlocking multilamellar (at least three layers) tissue weave weaves in the protein matter that contains normal skin and/or dermal tissue extracellular matrix components and/or the non-protein matter again and obtain bionic dermis as claimed in claim 1.
10. the application of bionic dermis as claimed in claim 1 in the graft of preparation reparation damaged skin or corium.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810040668.3A CN101628132B (en) | 2008-07-17 | 2008-07-17 | Bionic dermis and preparation method and application thereof |
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| CN200810040668.3A CN101628132B (en) | 2008-07-17 | 2008-07-17 | Bionic dermis and preparation method and application thereof |
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| CN101628132A true CN101628132A (en) | 2010-01-20 |
| CN101628132B CN101628132B (en) | 2014-12-03 |
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| CN200810040668.3A Expired - Fee Related CN101628132B (en) | 2008-07-17 | 2008-07-17 | Bionic dermis and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111330066A (en) * | 2020-04-30 | 2020-06-26 | 西安交通大学医学院第一附属医院 | Three-dimensional structured biological dressing for repairing skin lesion of severe patient |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2322415Y (en) * | 1998-03-17 | 1999-06-09 | 黄向先 | Tai Ji bionic undergarment |
| CN1324254A (en) * | 1998-09-24 | 2001-11-28 | 韩国原子力研究所 | Dermal scaffold comprising nutralized chitosan sponge, nutralized chitosan/collagen mixed sponge |
| CN1378445A (en) * | 1999-08-06 | 2002-11-06 | 得克萨斯系统大学评议会 | Drug releasing biodegradable fiber implant |
| CN1730263A (en) * | 2005-08-23 | 2006-02-08 | 刘正辉 | Method for manufacturing artificial bionic snake skin |
| CA2652007A1 (en) * | 2006-05-12 | 2007-11-22 | Smith & Nephew Plc | Scaffold |
| CN101411896A (en) * | 2007-10-15 | 2009-04-22 | 上海交通大学医学院附属瑞金医院 | Bionic dermis as well as preparation method and application thereof |
-
2008
- 2008-07-17 CN CN200810040668.3A patent/CN101628132B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2322415Y (en) * | 1998-03-17 | 1999-06-09 | 黄向先 | Tai Ji bionic undergarment |
| CN1324254A (en) * | 1998-09-24 | 2001-11-28 | 韩国原子力研究所 | Dermal scaffold comprising nutralized chitosan sponge, nutralized chitosan/collagen mixed sponge |
| CN1378445A (en) * | 1999-08-06 | 2002-11-06 | 得克萨斯系统大学评议会 | Drug releasing biodegradable fiber implant |
| CN1730263A (en) * | 2005-08-23 | 2006-02-08 | 刘正辉 | Method for manufacturing artificial bionic snake skin |
| CA2652007A1 (en) * | 2006-05-12 | 2007-11-22 | Smith & Nephew Plc | Scaffold |
| CN101411896A (en) * | 2007-10-15 | 2009-04-22 | 上海交通大学医学院附属瑞金医院 | Bionic dermis as well as preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111330066A (en) * | 2020-04-30 | 2020-06-26 | 西安交通大学医学院第一附属医院 | Three-dimensional structured biological dressing for repairing skin lesion of severe patient |
| CN111330066B (en) * | 2020-04-30 | 2022-05-20 | 西安交通大学医学院第一附属医院 | Three-dimensional structured biological dressing for repairing skin lesion of severe patient |
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| Publication number | Publication date |
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| CN101628132B (en) | 2014-12-03 |
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