CN101623326B - Sculellaria barbata formulation and preparation method thereof - Google Patents
Sculellaria barbata formulation and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of medicaments, in particular to a sculellaria barbata formulation capable of resisting tumor and virus and improving the immunity of engine bodies and a preparation method thereof. The sculellaria barbata formulation uses a sculellaria barbata general flavone extract or extracting solution as the main ingredient; the general flavone in the sculellaria barbata general flavone extract accounts for more than 75 percent, and the general flavone in the sculellaria barbata general flavone extracting solution accounts for more than 5 percent. The Chinese herba formulation of the sculellaria barbata general flavone is pure Chinese herba formulation, therefore, the invention avoids anaphylactic reaction and toxic side effect caused by taking and injecting Western medicine, has the advantages of simple and advanced preparation technique, economy and practicality, and is suitable for industrial production.
Description
Technical field
The present invention relates to the preparation of Chinese medicine extraction separation and preparation, the clinical a kind of Herba Scutellariae Barbatae extract and preparation method thereof of saying so more specifically with antitumor, antiviral, human body immunity improving power, reaching with this extract is the various Chinese medicine preparation that main component is made.Belong to medical technical field.
Background technology
The Herba Scutellariae Barbatae distributed resource is extremely abundant, is used for clinical existing centuries history in China, but in the past because shortage to the research of its effective substance and mechanism, has influenced its development and utilization.Herba Scutellariae Barbatae mainly contains constituents such as flavone, polysaccharide, sterol, organic acid, alkaloid.Total flavones in the Herba Scutellariae Barbatae especially height of scutellarin content has dependency with its curative effect.Therefore, the flavonoid chemical constituent has great importance in the effective development and utilization Herba Scutellariae Barbatae.
Based on above-mentioned purpose, we have from clinical that the side of tearing open filters out effective antitumor, antiviral, human body immunity improving power medicine Herba Scutellariae Barbatae the efficacious prescriptions medicine, extensive studies has been carried out in aspects such as its effective chemical analysis, pharmacological effect and the mechanism of action, developed single Herba Scutellariae Barbatae total flavones preparation on this basis.
Summary of the invention
Technical problem to be solved by this invention is will provide a kind of kinds of tumor cells is had the growth inhibited effect, and normal structure is not had injury, has antiviral, an effect of human body immunity improving power simultaneously, and mass ratio is easier to control, the Herba Scutellariae Barbatae extract preparation that general flavone content is high is to improve clinical efficacy.
Another object of the present invention is that it is the preparation technology of the various Chinese medicine preparation of main active with Herba Scutellariae Barbatae extract effective site that preparation is provided.
For addressing the above problem, the present invention has studied and defined following technical scheme:
A kind of Herba Scutellariae Barbatae is anticancer, antiviral, human body immunity improving power preparation, is to be main component with Herba Scutellariae Barbatae total flavones extract or extracting solution; General flavone content is greater than 75% in the said Herba Scutellariae Barbatae total flavones extract; General flavone content is greater than 5% in the said Herba Scutellariae Barbatae total flavones extracting solution.
Be rich in flavones ingredients (seeing accompanying drawing 3～5) such as scutellarin, rutin in above-mentioned Herba Scutellariae Barbatae total flavones extract or the extracting solution.In the described Herba Scutellariae Barbatae total flavones extract, scutellarin content is more than 25%～40%.In the described Herba Scutellariae Barbatae total flavones extracting solution, scutellarin content is more than 1.5%～3%.
The preparation method and the step of Herba Scutellariae Barbatae preparation of the present invention are as follows:
(1) total flavones extracting solution preparation: with the coarse pulverization of Herba Scutellariae Barbatae herb, with 0.1%～0.3% (W/W) arginine aqueous solution (pH6.0～8.5); Soak at room temperature, stir to extract, pH value is 7～9 Herba Scutellariae Barbatae total flavones extracting solution, its general flavone content is greater than 5%;
(2) extractive of general flavone preparation: the extracting solution that step (1) obtains is transferred pH2～3 or transferred pH3～4 to place with 10% (W/W) hydrochloric acid with 20% (W/W) acetic acid, and separation, washing precipitation are the Herba Scutellariae Barbatae total flavones extract, and its general flavone content is more than 75%;
(3) the Herba Scutellariae Barbatae total flavones extract of step (2) and Herba Scutellariae Barbatae nano powder are respectively 15%～55% and 45%～85% by mass ratio and are mixed and made into oral solid formulation;
Or: with the Herba Scutellariae Barbatae water extract that step (1) obtains, the micron filter membrane filters removal impurity and makes liquid preparation; Herba Scutellariae Barbatae total flavones content is 5%～35% in the oral liquid;
Or: the Herba Scutellariae Barbatae total flavones extract of step (2) is added the dissolving of arginine aqueous solution, and the micron membrane filtration is made oral liquid, and Herba Scutellariae Barbatae total flavones content is 5%～35% in the oral liquid.
Said oral solid formulation comprises granule, tablet, micropill among the present invention.The Herba Scutellariae Barbatae nano powder be the Herba Scutellariae Barbatae herb after micronizing particle diameter in 50nm～150nm scope, plant cell 100% breaking cellular wall.
Said oral liquid comprises oral liquid, mixture among the present invention, and Herba Scutellariae Barbatae total flavones content is 5%～35% in the oral liquid.
The prepared Herba Scutellariae Barbatae total flavones extractive content assay method of the present invention comprises one or both in the following method:
A, total flavones
Get the scutellarin reference substance, add methanol and make the solution that every 1ml contains 0.28mg, shake up, promptly get reference substance solution; The accurate reference substance solution 0.4 of drawing, 0.8,1.2,1.6,2.0ml, adding 0.3% arginine solution respectively to 25ml, is blank with 0.3% arginine solution, according to spectrophotography (an appendix VI of Chinese Pharmacopoeia version in 2005 A), wavelength place at 327nm measures trap, with concentration is abscissa, and trap is a vertical coordinate, the drawing standard curve; The accurate Herba Scutellariae Barbatae total flavones extracting solution of drawing is a blank with 0.3% arginine solution, according to spectrophotography (an appendix VI of Chinese Pharmacopoeia version in 2005 A), measures trap at the wavelength place of 327nm, calculates, promptly; This Herba Scutellariae Barbatae total flavones extract contains total flavones with scutellarin (C
21H
18O
12) meter, must not be less than 75%.
B, scutellarin
Measure according to high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 D).With octadecylsilane chemically bonded silica is filler, and volume ratio is 35: 61: 2 a methanol: water: acetic acid is mobile phase, and the detection wavelength is 327nm; Number of theoretical plate is pressed the scutellarin peak and is calculated, and is not less than 1500; Precision takes by weighing the scutellarin reference substance, adds mobile phase and makes the solution that contains scutellarin 0.88mg among every 1ml, shakes up, and promptly gets reference substance solution; Get this Herba Scutellariae Barbatae total flavones extract as need testing solution; Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid, measure, and calculate, promptly; This Herba Scutellariae Barbatae total flavones extract contains scutellarin must not be less than 25%.See accompanying drawing 4～5.
Oral formulations of the present invention, being used for clinical dosage is to take every day by raw medicinal herbs 30～60g.
The invention has the advantages that:
(1) the Herba Scutellariae Barbatae total flavones Chinese medicine preparation is that the effective ingredient of single medicinal material is formed, be a kind of be the antitumor drug of action target spot to suppress revascularization, and the effect of antiviral, human body immunity improving power is arranged.Show that through animal experimental observation product degree of absorbing is good, have no adverse reaction, good effect, economic security.
(2) the selection of material science of the present invention, preparation technology is simple advanced, economical and practical, is suitable for suitability for industrialized production.Because major part is a flavonoid glycoside in the Herba Scutellariae Barbatae total flavones, contains the flavonoid glycoside metaclass on a small quantity or not, very easily is dissolved in alkaline solution.Consider the unstability of flavone compound simultaneously to soda acid.So the present invention has used a kind of unprecedented effective extracting method, and the arginine aqueous solution is extracted solvent as total flavones.Also with the crude drug nano-pulverization, also be one of innovation among the present invention.
(3) the Herba Scutellariae Barbatae total flavones extracting solution is through experimental verification, and active carbon has intensive adsorption to flavones ingredient in the Herba Scutellariae Barbatae, so do not select charcoal treatment for use in preparation technology.
(4) Herba Scutellariae Barbatae total flavones Chinese medicine preparation of the present invention is a pure Chinese medicinal preparation, has therefore avoided taking and injecting anaphylaxis and some toxic and side effects that Western medicine brings.Undue toxicity's check result shows that Herba Scutellariae Barbatae total flavones solution is qualified to the mice abnormal toxicity test; Systemic allergy test is the result show, Herba Scutellariae Barbatae total flavones solution does not have systemic anaphylaxis to Cavia porcellus; Irritation test is the result show, the local irritation test is up to specification.
Description of drawings:
Fig. 1 is the shadow noon contrast figure that the Herba Scutellariae Barbatae flavone extract forms the carefully full tubule of HUVEC endothelium; A wherein: contrast; B～D:25,50,100mg/L Herba Scutellariae Barbatae flavone extract
Fig. 2 is that the Herba Scutellariae Barbatae flavone extract is to carefully full shadow noon of moving of endothelium; A wherein: contrast; B～D:25,50,100mg/L Herba Scutellariae Barbatae flavone extract
Fig. 3 is a rutin standard substance HPLC collection of illustrative plates.
Fig. 4 is a scutellarin standard substance HPLC collection of illustrative plates.
Fig. 5 is a Herba Scutellariae Barbatae flavone extractive HPLC collection of illustrative plates.
The specific embodiment
Embodiment 1: the preparation example of various pharmaceutical preparatioies
(1) total flavones extracting solution preparation: with the coarse pulverization of Herba Scutellariae Barbatae herb, with 0.1%～0.3% (W/W) arginine aqueous solution (pH6.0～8.5); Soak at room temperature, stir to extract, pH value is 7～9 Herba Scutellariae Barbatae total flavones extracting solution, its general flavone content is greater than 5%;
(2) extractive of general flavone preparation: the extracting solution that step (1) obtains is transferred pH2～3 or transferred pH3～4 to place with 10% (W/W) hydrochloric acid with 20% (W/W) acetic acid, and separation, washing precipitation are the Herba Scutellariae Barbatae total flavones extract, and its general flavone content is more than 75%;
(3) preparation granule and tablet:
Take by weighing the Herba Scutellariae Barbatae total flavones extract of 15%-55% by the configuration total amount, the Herba Scutellariae Barbatae nano powder that adds 45%-85%, fully mix, make adhesive, make soft material with 70% (V/V) ethanol, cross 14 order nylon screens and make granule, (this wet granular directly can be rolled into micropill in coating pan, need not to add any adjuvant), wet granular was in 60 ℃ of dryings 2～3 hours, dried particles is crossed 14 mesh sieve granulate, makes every gram granule contain Herba Scutellariae Barbatae total flavones extract 200～600mg and both gets.
This dried granule directly can be pressed into tablet.
Preparation oral liquid or mixture:
Take by weighing 15%～55% Herba Scutellariae Barbatae total flavones extract by the configuration total amount, add 0.3% arginine aqueous solution dissolved dilution to certain volume, regulate pH6.5～8, cold preservation 24 hours filters, and filtrate adds water and adjusts the discharge standard of total amount amount of showing, stir evenly, 0.22 the micron filter membrane filters, fill promptly makes every milliliter of oral liquid contain Herba Scutellariae Barbatae total flavones extract 50～300mg.Or directly the Herba Scutellariae Barbatae total flavones extracting solution is adopted 100 ℃ of heating of flowing steam 30min, and 24h is placed in 4 ℃ of cold preservations, filters through 0.22 micron filter membrane, and allotment makes every milliliter of oral liquid contain Herba Scutellariae Barbatae total flavones extract 50～300mg fill promptly.
Embodiment 2: the determination of total flavonoids method
(1) establishment of uv absorption wavelength
Precision takes by weighing the scutellarin reference substance, add 0.3% arginine aqueous solution (pH8.5), making concentration is the scutellarin reference substance solution of 0.028mg/mL, carry out the long (190nm～900nm) scanning of ultraviolet all-wave, scutellarin respectively has an absworption peak at λ=277nm and λ=327nm place as a result, the ultra-violet absorption spectrum basically identical of the ultra-violet absorption spectrum of Herba Scutellariae Barbatae total flavones extracting solution and scutellarin.Experiment showed, that other liposoluble constituent also has uv absorption at 277nm wavelength place in the Herba Scutellariae Barbatae medical material, bigger to this peak influence, other composition in the Herba Scutellariae Barbatae beyond the total flavones then absorbs very little at 327nm wavelength place.So determine to measure the trap of Herba Scutellariae Barbatae total flavones at the 327nm place.
(2) foundation of standard curve
Precision takes by weighing scutellarin reference substance 1.700mg, puts in the 10mL measuring bottle, adds 0.3% arginine aqueous solution (pH8.5) to scale, standardize solution.The accurate scutellarin reference substance solution 0.4,0.8,1.2,1.6 of drawing, 2.0ml places the 25ml measuring bottle respectively, adds 0.3% arginine aqueous solution (pH8.5) to scale, shakes up.By under the version Chinese Pharmacopoeia medium ultraviolet spectrophotography item in 2005, with 0.3% arginine aqueous solution (pH8.5) is blank, the place measures absorbance respectively at the 327nm wavelength, with absorbance (Y) is vertical coordinate, solution concentration (X) is an abscissa, and the drawing standard curve must regression equation be: Y=14.369x-0.0211, r=0.9994 (n=5), the result shows that Herba Scutellariae Barbatae total flavones is at 0.0027072-0.0135360mgml
-1Be good linear relationship.
(3) precision test
Same batch Herba Scutellariae Barbatae extracting solution is used the method duplicate detection 5 times, calculate the extracting solution general flavone content, RSD is 1.5%, and precision is good.
(4) stability test
Get above-mentioned reference substance solution of having measured and sample solution, place after 3,6,9,12 hours, through ultraviolet spectroscopy, its absorption value is constant substantially, illustrates that reference substance solution and sample solution all are very stable.
(5) application of sample recovery test
Precision takes by weighing the Herba Scutellariae Barbatae flavone extractive 1ml of known general flavone content, totally 9 parts, be divided into basic, normal, high 3 groups, add scutellarin reference substance solution (being equivalent to scutellarin 0.025,0.050,0.075mg) respectively, measure flavones content in the Herba Scutellariae Barbatae flavone extractive, calculate recovery rate, RSD is 1.87%, the results are shown in Table 1.
Table 1 average recovery result of the test
(6) sample determination
Get the Herba Scutellariae Barbatae flavone extractive for preparing, extracting solution is measured trap at the 327nm place, utilize the standard curve external standard method quantitative, calculate content of total flavone in the Herba Scutellariae Barbatae flavone extractive, the results are shown in Table 2.
Table 2 different batches Herba Scutellariae Barbatae flavone extractive determination of total flavonoids result
Embodiment 3: observation of curative effect
Material and method
(1) material
Human umbilical vein endothelial cells (HUVEC), s strain are provided by Yangzhou University's medical college Chinese medicine institute; Herba Scutellariae Barbatae flavone compound extract is provided by Yangzhou University's medical college Chinese medicine institute; Fibroblast growth factor (bFGF) and Matrigel are respectively available from Sigma and BD company; The ELISV test kit of people VEGF is available from Wuhan doctor's moral company; The NO test kit builds up bio-engineering research institute available from Nanjing; The two transfection reagent boxes of AnnexinV associating PI are available from Nanjing KaiJi Biology Science Development Co., Ltd; To be configured to the mother solution of 1g/L with dimethyl sulfoxide (DMSO), M199 and DMEM culture medium ,-20 ℃ of preservations behind the sucking filtration, experiment taking-up on the same day is diluted to the experiment desired concn; HUVEC and Hela cell be conventional cultivation the in incubator all, and to go down to posterity behind the trypsinization, the trophophase cell of taking the logarithm is used for experiment.Determine that by the MTT colorimetry IC10 of Herba Scutellariae Barbatae flavone extract is 104.08mg/L in preliminary experiment, integer concentration that selection and IC10 are approaching and multiple successively decrease as the concentration of experiment.
(2) method
1. the little tube formation assay of endotheliocyte: the every hole of 24 orifice plates added put 4 ℃ of Matrigel300 μ l that spend the night in advance, at 37 ℃, 50ml/LCO
2Polymerization 1h in the incubator transfers 2 * 10 with culture fluid with HUVEC after with trypsinization
8The cell suspension of/L concentration, and add 10 μ g/LbFGF and respectively 25,50, the Herba Scutellariae Barbatae flavone extract of 100mg/L, other establishes blank group (adding not dosing of cell in the hole) and solvent matched group (containing 0.1g/LDMSO in the culture fluid), and mixing is put into 37 ℃, 50ml/LCO
2Observe the situation that tubule forms after cultivating 6h in the incubator under 100 times of Olympus inverted microscopes, every hole is got 5 visual field counting tubules and is formed number, averages.
2. endothelial cell migration experiment: endotheliocyte is digested the back with 1 * 10
8/ L kind is gone into 24 orifice plates, adds the Herba Scutellariae Barbatae flavone extract in culture medium, and the medicine final concentration is 25,50,100mg/L, final volume 2ml, and establish totally 5 groups of negative control group and solvent matched groups, 37 ℃, 50ml/LCO
2Digest after cultivating 24h in the incubator, and be 1 * 10 with serum-free M199 culture medium adjustment density
8/ L.The Matrigel20 μ l that on the polycarbonate membrane of chamber on the Transwell, adds 3mg/L, 37 ℃ of polyase 13 0min, add 600 μ lHela cell conditioned mediums in the following chamber of Transwell, going up simultaneously the chamber plants respectively into the endotheliocyte with serum-free M199 dilution, every hole 100 μ l, take out cell after cultivating 18h, wipe the endotheliocyte that does not move on the filter membrane upper strata away with cotton swab, fixing, dyeing is taken off filter membrane with blade, resinene is fixed on the microscope slide, totally 5 visual field cell number in the middle of microscopically is counted at random and on every side, several 4 samples of each batch total are obtained meansigma methods.
3. the influence that Hela cell VEGE and NO are expressed: with behind the Hela cell dissociation with 3 * 10
8/ L concentration is planted 6 orifice plates, cultivate 24h after, clean 2 times with PBS, add DMEM culture medium and the medicine of 100mg/L NBS, final concentration is 25,50,100mg/L, final volume 2ml, 37 ℃, 50ml/LCO
2Collect culture supernatant after cultivating 24h and 48h respectively in the incubator, the centrifugal 10min of 12000r/min gets supernatant behind 0.22 μ m filter membrane sucking filtration, with VEGF and NO content in ELISA and the NO kit measurement supernatant.
Statistical procedures: adopt the analysis of SPSS 10.0 statistical softwares, experimental result is represented with mean ± SD, carries out variance analysis and t and tests.
(3) result
1. the Herba Scutellariae Barbatae flavone extract forms the endotheliocyte tubule and the influence of migration: after the low concentration effect of 25mg/L tubule being formed number does not have obviously influence, tubule formed when above and have a significant effect when concentration reaches 50mg/L, tubule decreased number not only, and tube chamber is imperfect, compare with the blank group variant (P＜0.05), when concentration reaches 100mg/L, seldom have complete tubule form (table 3, Fig. 1).HUVEC is through 50, after the Herba Scutellariae Barbatae flavone extract effect of 100mg/L, and the transport number of endotheliocyte obviously reduces (p＜0.01), and the endotheliocyte number of migration reduce along with the increase of concentration (table 3, Fig. 2).
Table 3 Herba Scutellariae Barbatae flavone extract the endotheliocyte tubule is formed and migration influence mean ± SD, n=4
aP＜0.05,
bP＜0.01, the vs matched group
Table 4 Herba Scutellariae Barbatae flavone extract is to the mean ± SD that influences of Hela emiocytosis VEGF and NO, n=4
bP＜0.01, the vs matched group
2. the Herba Scutellariae Barbatae flavone extract is to the influence of Hela emiocytosis VEGF and NO: Herba Scutellariae Barbatae flavone extract effect 24h, can suppress the expression (p＜0.01) of Hela cell VEGE behind the 48h, but relatively there is not difference between each group behind the effect 24h, behind the effect 48h, more variant between the middle and high concentration group (50,100mg/L), and express between 24h and the 48h more variant (p＜0.05) through the Hela cell VEGE after the effect of middle and high concentration group.50, the NO that can obviously suppress tumor cell behind the 100mg/L Herba Scutellariae Barbatae flavone extract function cells 24h expresses, has significant difference, and each concentration of more variant effect back all can suppress the expression of cell between each concentration group, there were significant differences (p＜0.01), and it is more variant between each concentration group, each concentration all can suppress the expression of Hela cell NO behind the effect 48h, there were significant differences (p＜0.01), each organizes same concentration system, 24h compares 50 with 48h, during 100mg/L NO is expressed influence and have significant difference (p＜0.01), other concentration are compared does not have significant difference (table 4).
(4) conclusion
The Herba Scutellariae Barbatae flavone compound can suppress the endothelial cell migration under formation of endotheliocyte tubule spline structure and the stimulation of tumor cell conditioned medium, experiment shows that its mechanism of action may can suppress tumor cell secretion NEGF with it and these angiogenesis promotions of NO are factor-related, lays a good foundation in the prospect aspect the oncotherapy for further inquiring into Herba Scutellariae Barbatae.
Claims (5)
1. a Herba Scutellariae Barbatae preparation is characterized in that, is to be main component with Herba Scutellariae Barbatae total flavones extract or extracting solution; Make by the following method:
(1) total flavones extracting solution preparation: with the coarse pulverization of Herba Scutellariae Barbatae herb, with pH6.0 ~ 8.5,0.1% ~ 0.3% arginine aqueous solution soak at room temperature, stir and extract, pH value is 7 ~ 9 Herba Scutellariae Barbatae total flavones extracting solution, its general flavone content is greater than 5%;
(2) extractive of general flavone preparation: the extracting solution of step (1) is transferred pH2 ~ 3 or transferred pH3 ~ 4 to place with 10% hydrochloric acid with 20% acetic acid, and separation, washing precipitation are the Herba Scutellariae Barbatae total flavones extract, and its general flavone content is more than 75%;
(3) the Herba Scutellariae Barbatae total flavones extract of step (2) and Herba Scutellariae Barbatae nano powder are respectively 15% ~ 55% and 45% ~ 85% by mass ratio and are mixed and made into oral solid formulation;
Or: with the Herba Scutellariae Barbatae water extract that step (1) obtains, the micron filter membrane filters removal impurity and makes liquid preparation; Herba Scutellariae Barbatae total flavones content is 5% ~ 35% in the oral liquid;
Or: the Herba Scutellariae Barbatae total flavones extract of step (2) is added the dissolving of arginine aqueous solution, and the micron membrane filtration is made oral liquid, and Herba Scutellariae Barbatae total flavones content is 5% ~ 35% in the oral liquid.
2. by the described Herba Scutellariae Barbatae preparation of claim 1, it is characterized in that, contain scutellarin, rutin in described Herba Scutellariae Barbatae total flavones extract or the extracting solution.
3. by the described Herba Scutellariae Barbatae preparation of claim 2, it is characterized in that in the described Herba Scutellariae Barbatae total flavones extract, scutellarin content is 25% ~ 40%.
4. by the described Herba Scutellariae Barbatae preparation of claim 2, it is characterized in that in the described Herba Scutellariae Barbatae total flavones extracting solution, scutellarin content is 1.5% ~ 3%.
5. the preparation method of the preparation of Herba Scutellariae Barbatae described in the claim 1 is characterized in that:
(1) total flavones extracting solution preparation: with the coarse pulverization of Herba Scutellariae Barbatae herb, with pH6.0 ~ 8.5,0.1% ~ 0.3% arginine aqueous solution soak at room temperature, stir and extract, pH value is 7 ~ 9 Herba Scutellariae Barbatae total flavones extracting solution, its general flavone content is greater than 5%;
(2) extractive of general flavone preparation: the extracting solution of step (1) is transferred pH2 ~ 3 or transferred pH3 ~ 4 to place with 10% hydrochloric acid with 20% acetic acid, and separation, washing precipitation are the Herba Scutellariae Barbatae total flavones extract, and its general flavone content is more than 75%;
(3) the Herba Scutellariae Barbatae total flavones extract of step (2) and Herba Scutellariae Barbatae nano powder are respectively 15% ~ 55% and 45% ~ 85% by mass ratio and are mixed and made into oral solid formulation;
Or: with the Herba Scutellariae Barbatae water extract that step (1) obtains, the micron filter membrane filters removal impurity and makes liquid preparation; Herba Scutellariae Barbatae total flavones content is 5% ~ 35% in the oral liquid;
Or: the Herba Scutellariae Barbatae total flavones extract of step (2) is added the dissolving of arginine aqueous solution, and the micron membrane filtration is made oral liquid, and Herba Scutellariae Barbatae total flavones content is 5% ~ 35% in the oral liquid.
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