CN101613382B - New geranium extract - Google Patents
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Abstract
The invention relates to a new geranium extract and a preparation method thereof, in particular to the geranium extract containing corilagin, and application of the geranium extract in medicaments for treating or preventing gastroxia and related diseases.
Description
Technical field
The present invention relates to a kind of new Herba Erodii extract and preparation method thereof; Relate to the Herba Erodii extract that contains corilagin particularly; The purposes that also relates to said Herba Erodii extract gastric acid inhibitory secretion, reduction gastric acidity, and the purposes of treating or preventing diseases such as hyperchlorhydria, peptide ulceration, chronic gastritis.
Background technology
Herba Erodii is China's medicinal material commonly used; It is of a great variety; Comprise yak seedling section yak seedling platymiscium yak seedling (Erodium stephanianum Willd) and equal Geranium plant Herba Erodii (Geraniumwilfordii Maxim), and nepal cranesbill (Geranium nepalense Sweet.), thick root Herba Erodii (G.dahuricum DC), Pelargonium (G.sibiricum L.), Carolina Cranesbill Herb (G.caroliniznum L.), Herba Geranii eriostemonis (G.eriostemon Fisch.ex.DC.), suede back of the body Herba Erodii (Geranium vlassovianum), Qingdao Cranesbill (Geranium tsingtauense Yabe), Robetium macrorrhizum (Geranium macrorrhizum) and geranium thunbergii (G.thunbergii Sieb.et Zucc.) etc.Chemical ingredients in the Herba Erodii is numerous, mainly comprises tannin, flavonoid, organic acid and volatilization wet goods.Along with people's deepens continuously to Herba Erodii research, and its increasing pharmacological action constantly comes to light, like anti-inflammatory, analgesic activity, anti-microbial effect, antivirus action; Antioxygenation, immunization, antitussive effect, anti-diarrhea effect; Hepatoprotective effect, antitumor action, blood sugar reducing function, anastalsis etc.Yet also do not see the active relevant reports in aspect such as the secretion of Herba Erodii gastric acid inhibitory, reduction gastric acidity.The contriver is engaged in the experimental study of Herba Erodii for a long time, finds that Herba Erodii has the gastric acid inhibitory secretion, reduces the activity of gastric acidity, aspect the diseases such as treatment or prevention hyperchlorhydria, peptide ulceration, chronic gastritis good effect is being arranged.
Hyperchlorhydria is meant gastric juice (comprising compositions such as hydrochloric acid and stomach en-) supersecretion, and makes the patient feel to have a stomach upset, sour regurgitation water, heartburn (stomach burning sensation).Slight hyperchlorhydria mainly shows as and occurs stomach burning sensation, sour regurgitation repeatedly; If hyperchlorhydria infringement stomach mucous membrane; Also can cause stomachache, feel sick, symptom such as vomiting, hyperchlorhydria also possibly be further developed into peptide ulceration, even perforated ulcer occurs.
Peptide ulceration is a kind of global common disease and frequently-occurring disease, estimates that about 10% crowd was once suffering from this disease in life.Peptide ulceration mainly means stomach ulcer and duodenal ulcer; It is a kind of multi-pathogenesis disease; Its morbidity is relevant with multiple factor, like hydrochloric acid in gastric juice, stomach en-, Hp (Hp) infection, heredity, physique, environment, diet, life style, neural mental element etc., through different approaches or mechanism; Cause above-mentioned invasion and attack effect strengthen with or preventing mechanism weaken, all can inspire ulcer and take place.Along with people's deepens continuously to hydrochloric acid in gastric juice generting machanism and gastric mucosal defense function understanding; This treatment of diseases method is also being updated, at present main treat-ment have following method (referring to: the time space, the treatment of stomach ulcer and gastric acid secretion; The biology circular; 1994,29 (12), 14-15):
Antiacid pharmacotherapy:
At present, generally acknowledge that it is satisfied ulcer healing environment and indispensable treatment condition that stomach inner pH value maintains more than the 3.5-4.0.Therefore gastric acid inhibitory secretion, improving the interior pH of stomach is the basis of treatment of peptic ulcer.Antacid generally is an alkaline drug, like sodium bicarbonate etc., through reducing acidity in the stomach with hydrochloric acid generation neutralization reaction; But this neutral effectiveness can only temporarily be lived in peace with each other, and hydrochloric acid in gastric juice again can supersecretion soon, if neutralize repeatedly with alkaline medicine again; Can cause that more hydrochloric acid in gastric juice secretes in a large number, be as good as and add fuel to the flames.This is because this type medicine can only neutralize and be secreted into the hydrochloric acid in gastric juice of gastral cavity; Result's gastral cavity pH value at short notice raises rapidly; This has weakened the effect of the negative feedback inhibition gastric acid secretion of hydrochloric acid in gastric juice on the contrary; Make parietal cell generate more hydrochloric acid in gastric juice, cause " knock-on ", so antacid only is used as the adjuvant drug of ulcer treatment at present.
The receptor blocking agent therapy:
In vivo, have three kinds of materials can directly act on parietal cell, promote the generation and the secretion of hydrochloric acid in gastric juice, they are respectively: vagusstoff, gastrin, I4EA.When it is found that these three kinds of physiological stimuluss all are to act on the acceptor on the parietal cell film and behind the gastric acid secretion, just attempt to seek the corresponding receptors blocker, the acceptor with on the blocking-up parietal cell film reduces the generation of hydrochloric acid in gastric juice.Being used for clinical the earliest is cholinergic receptor blocker coromegine (atropine).Coromegine can only reduce the generation of a part of hydrochloric acid in gastric juice, and the patient is effective to part.But, thereby spinoffs such as dry, the dimness of vision are arranged because of coromegine also acts on the m receptor of other part of body simultaneously.In recent years, the discovery of specific M receptor blocker pirenzepine (pirenzepine) has substituted atropinic use, and effect is better than coromegine.H receptor blocker mainly contains medicines such as Cimitidine Type A/AB, Ranitidine HCL, famotidine, nizatidine, roxatidine, and they are through the secretion of blocking-up parietal cell H2 acceptor and gastric acid inhibitory, has characteristics such as determined curative effect, untoward reaction are few, economical and practical.Though gastrin-receptor is still at the experimental stage at present through seeking for many years, is not used in clinical treatment.Because three kinds of stimuluss independently play a role through each autoreceptor, thereby the use receptor blocking agent is treated, can only be to a certain extent or only effective to a part of patient.
Proton pump inhibitor (PPI) therapy:
Because proton pump is the final step that produces hydrochloric acid in gastric juice, thereby reducing the effective means of hydrochloric acid in gastric juice is the activity that suppresses proton pump.The proton pump inhibitor such as the omeprazole (Omeprazole) of development can directly act on proton pump in recent years, suppress the effect of its secretion hydrochloric acid in gastric juice.Omeprazole has been widely used in clinical treatment abroad, and effect is fine.But because stomach ulcer is a kind of chronic disease, and sour pump metabolism is very fast, must adhere to taking for a long time omeprazole, and this causes treating the cost costliness, has exceeded many patients' ability to bear, and therefore, this medicine also is not suitable for extensive at home clinical use.
Eradicate the pharmacological agent of Hp:
Should accept the eradication therapy of Hp to Hp male patient, main therapy is to unite to use microbiotic and antacid.Microbiotic such as clarithromycin, amoxycilline Trihydrate bp, tinidazole etc. play antibacterial or germicidal action, but these microbiotic are all not acidproof, and its drug effect depends on the pH value; Acid inhibitor such as PPI can provide a suitable pH environment, and many acid nonfast microbiotic will be brought into play maximum effectiveness under this environment.
Chronic gastritis also is a kind of common disease, frequently-occurring disease, and it is the first that its sickness rate occupy in various stomach trouble, accounts for outpatient service gastroscopy patient's 90%, and the age is big more, and sickness rate is high more, and particularly middle-older patient more more sees.The general treatment method of chronic gastritis normally adopts the proton pump inhibitor treatment, reduces the stimulation of hydrochloric acid in gastric juice to stomach mucous membrane, mitigation symptoms; Or the pharmacological agent of colloidal bismuth class, this type of medicine is owing to its colloid property, and the protein binding that can under sour environment, ooze out with mucous membrane forms firm protective together on stomach mucous membrane; Or antibiotics or other non-medicine assisting therapy etc.(referring to: 1, Zhang Yan, the treatment present situation of chronic gastritis, the 24th the 4th phase of volume of Li Ganzhi's journal; 2, Guo Shoujun, the diagnosis and treatment of chronic gastritis, Chinese clinic study magazine, the 13rd the 2nd phase of volume of January in 2007).
In sum, diseases such as hyperchlorhydria, peptide ulceration, chronic gastritis are common frequently-occurring disease, and their treatment all need reduce acidity in the stomach mostly, and continuing to seek safely and effectively, the broad-spectrum curing medicine is still the problem that needs solve.The contriver is through experimental study for many years; Find that unexpectedly a kind of Herba Erodii extract has the activity of good restraining gastric acid secretion, reduction gastric acidity; Aspect the diseases such as treatment or prevention hyperchlorhydria, peptide ulceration, chronic gastritis good effect is being arranged, and accomplishing the present invention based on above discovery.
Summary of the invention
One aspect of the present invention provides a kind of Herba Erodii extract, and the content of corilagin is 5%-40% in the said Herba Erodii extract, preferred 10%-30%, most preferably 20%-30%.
The present invention provides the described preparation method who contains the Herba Erodii extract of corilagin on the other hand, it is characterized in that: it is some to get the Chinese medicine Herba Erodii, pulverizes; Add 5-10 times of water gaging, heating decocts 2-4 time, each 1.5 hours; United extraction liquid filters, and collects filtrating; With the macroporous adsorptive resins of above-mentioned filtrating, carry out dynamic adsorption through having handled well.After upper prop was accomplished, resin column was eluted to the elutriant terminal point with the 1-4 times of arbitrary concentration ethanol of amount of resin 70-95% again and ends with the water flushing of 3-8 times of amount of resin, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to doing the Herba Erodii extract that promptly obtains containing corilagin.Wherein the macroporous adsorptive resins model can be selected from HPD100, HPD400, HPD600 and be preferably HPD400.
Preferred process for extracting is: it is some to get the Chinese medicine Herba Erodii, pulverizes, and adds 7 times of water gagings, and heating decocts 2 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD400 macroporous adsorptive resins of above-mentioned sample liquid through having handled well.After upper prop was accomplished, resin column ended with 2 times of amount of resin 95% ethanol elution to elutriant terminal points with the water flushing of 5 times of amount of resin again, collects alcohol eluen respectively.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating promptly gets the Herba Erodii extract that contains corilagin to doing.
The present invention's all " doubly amounts " all refers to the multiple relation of quality.
Further aspect of the present invention provides the said Herba Erodii extract gastric acid inhibitory secretion that contains corilagin, reduces the purposes of gastric acidity.
Further aspect of the present invention provides the said Herba Erodii extract treatment of corilagin or the purposes of prevention hyperchlorhydria of containing.
Further aspect of the present invention provides the said Herba Erodii extract treatment of corilagin or the purposes of prevention peptide ulceration of containing.Described peptide ulceration comprises stomach ulcer and duodenal ulcer.
Further aspect of the present invention provides the said Herba Erodii extract treatment of corilagin or the purposes of prevention chronic gastritis of containing.
Further aspect of the present invention provides a kind of pharmaceutical composition, comprises the described Herba Erodii extract that contains corilagin and one or more proper supplementary material pharmaceutically.
Herba Erodii described in the present invention mainly is selected from yak seedling section yak seedling platymiscium yak seedling (Erodium stephanianum Willd) and the thick root Herba Erodii of equal Geranium plant (Geraniumdahuricum); Carolina Cranesbill Herb (Geranium caroliniznum); Herba Geranii eriostemonis (Geraniumeriostemon); Suede back of the body Herba Erodii (Geranium vlassorianum); Qingdao Cranesbill (Geraniumtsingtauense Yabe); Robetium macrorrhizum (Geranium macrorrhizum) waits one or more plant.
The content of corilagin adopts HPLC to detect in the Herba Erodii extract provided by the invention, and actual conditions and method are following:
Instrument: high performance liquid chromatograph
Pump: Tianjin, island LC-10AT vp
Detector: Tianjin, island SPD-10A vp
Workstation: the NC2000 of Zhejiang University
Moving phase: acetonitrile-1% phosphoric acid solution (13: 87)
Wavelength X: 268nm
Chromatographic column: Thermo Hypersil BDS C18 (4.6 * 250mm, 5 μ m)
Flow velocity: 1.0ml/min
Column temperature: 30 ℃
Reference substance: corilagin (method according among the Chinese patent ZL97104154.7 makes), it is 95% that area normalization method is measured content
Method of calculation: the about 20 μ g/ml of reference substance solution concentration (C to) prepare the about 100 μ g/ml of strength of solution to be measured (C appearance), sample size 20 μ l, record reference substance solution peak area (A to), solution peak area to be measured (A appearance)
Pharmaceutical composition provided by the invention can be Herba Erodii extract and the mixing acceptable accessories that contains corilagin; Pharmaceutical preparation technology according to routine; Any pharmaceutical prepn of use, for example capsule, tablet, pill, granule, the oral liquid etc. clinically of being fit to that are prepared into.
Pharmaceutically proper supplementary material comprises pharmaceutically acceptable weighting agent, disintegrating agent, tackiness agent and lubricant, correctives etc.
Suitable weighting agent such as lactose, Microcrystalline Cellulose, pregelatinized Starch, starch, dextrin, N.F,USP MANNITOL, Mierocrystalline cellulose, sucrose, glucose, sorbyl alcohol, Xylitol etc.
Suitable disintegrants such as PVPP, sodium starch glycolate, low-substituted hydroxypropyl cellulose, crosslinked Vltra tears etc.
Suitable binder such as polyvidone, hydroxypropylcellulose, methylcellulose gum, Xylo-Mucine, Vltra tears, TKK 021 etc.
Examples of suitable lubricants such as micropowder silica gel, Magnesium Stearate, Zinic stearas, calcium stearate, Triple Pressed Stearic Acid, refining Wecobee M, palmitinic acid, talcum powder, polyoxyethylene glycol, sodium lauryl sulphate, Stepanol MG etc.
Suitable correctives such as sucrose, simple syrup, glycerine, sorbyl alcohol, N.F,USP MANNITOL, stevioside, soluble saccharin etc.
The present invention is through ligation rat pylorus; Collect 6 hours excretory gastric juice amounts and acidity and estimate the said influence that contains the Herba Erodii extract of corilagin to gastric acid secretion; Test-results shows; The said Herba Erodii extract that contains corilagin can effectively suppress the gastric acid secretion of rat, reduces gastric acidity.When the result of treatment of estimating peptide ulceration, adopt following three kinds of experimental animal models: pyloric ligation ulcers rat gastric ulcer model, water logging irritability rat gastric ulcer model, the acetate type rat gastric ulcer model of burning.Wherein, pylorus ligature law is a Shay ' s traditional classical rat gastric ulcer modelling, and behind the ligation pylorus, gastric juice is detained in the stomach, and the coat of the stomach defence capability weakens, and causes stomach ulcer to form.Microscopically is observed and is seen leukocyte infiltration and tissue necrosis, and ulcer reaches the flesh layer deeply.This model is similar with the stomach ulcer that human hyperchlorhydria causes.The modeling mechanism of water logging irritability rat gastric ulcer model does, after animal received stress stimulation, the sympathetic nervous system irritability raise, and vasoconstriction causes the mucous membrane hypoxic-ischemic, and resistibility descends.Parasympathetic nerve-hypophysis-adrenal system is excited to raise, and causes that hydrochloric acid in gastric juice, stomach en-and gastrin secretion increase, thereby causes stress ulcer.The acetate method is directly burnt the stomach serous coat with acetate and is formed ulcer, and this method is reliable, good reproducibility, and ulcer is dark and big, and is very similar with the mankind's chronic gastric ulcer.Its normal healing needs about 60 days, individual animal is also arranged in still disunion more than 200 days, thereby is applicable to the effect of observing medicine promotion ulcer healing, can be used as the experimental model of screening treatment chronic gastric ulcer medicine.
Animal test results shows: the Herba Erodii extract that contains corilagin provided by the invention can reduce gastric acid secretion, reduces ulcer incidence and the UI of rat in pyloric ligation ulcers and the water logging stress gastric ulcer model; And the healing of rat gastric ulcer in the acetate burn type gastric ulcer model had significant promoter action, and can increase the ulcer healing percentage, reduce UI.
The Herba Erodii extract preparation technology who contains corilagin provided by the invention is simple; And can reduce gastric acid secretion; Reduce gastric acidity, reduce the incidence and the UI of ulcer, have the effect of diseases such as good curing or prevention hyperchlorhydria, peptide ulceration, chronic gastritis.And can be prepared into the various kinds of drug compsn easily according to the ordinary method of this area for clinical use.
Embodiment
Specify content of the present invention with specific embodiment below, following examples also do not mean that any restriction of the present invention.
Embodiment 1 preparation contains the Herba Erodii extract of corilagin
Get Robetium macrorrhizum (Geranium macrorrhizum) 2000g, pulverize, add 7 times of water gagings, heating decocts 2 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD400 50kg macroporous adsorptive resins of above-mentioned sample liquid through having handled well.After upper prop was accomplished, resin column ended with 2 times of amount of resin 95% ethanol elution to elutriant terminal points with the water flushing of 5 times of amount of resin again, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to the dried Herba Erodii extract 44g that obtains containing corilagin, and the content of corilagin is 37% to use HPLC to detect wherein.
Embodiment 2 preparations contain the Herba Erodii extract of corilagin
Get thick root Herba Erodii (Geranium dahuricum DC) 2000g, pulverize, add 8 times of water gagings, heating decocts 3 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD40050kg macroporous adsorptive resins of above-mentioned sample liquid through having handled well.After upper prop was accomplished, resin column ended with 4 times of amount of resin 90% ethanol elution to elutriant terminal points with the water flushing of 8 times of amount of resin again, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to the dried Herba Erodii extract 38g that obtains containing corilagin, and the content of corilagin is 23% to use HPLC to detect wherein.
Embodiment 3 preparations contain the Herba Erodii extract of corilagin
Get Carolina Cranesbill Herb (Geranium caroliniznum) and pulverize, add 10 times of water gagings, heating decocts 3 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD40050kg macroporous adsorptive resins of above-mentioned filtrating through having handled well.After upper prop was accomplished, resin column ended with 2 times of amount of resin 70% ethanol elution to elutriant terminal points with the water flushing of 6 times of amount of resin again, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to doing the Herba Erodii extract 40g that had both obtained containing corilagin, and the content of corilagin is 29% to use HPLC to detect wherein.
Embodiment 4 preparations contain the Herba Erodii extract of corilagin
Get Herba Geranii eriostemonis (Geranium eriostemon) 2000g, pulverize, add 5 times of water gagings, heating decocts 2 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD400 50kg macroporous adsorptive resins of above-mentioned filtrating through having handled well.After upper prop was accomplished, resin column ended with 4 times of amount of resin 95% ethanol elution to elutriant terminal points with the water flushing of 5 times of amount of resin again, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to doing the Herba Erodii extract 43g that had both obtained containing corilagin, and the content of corilagin is 14% to use HPLC to detect wherein.
Embodiment 5 preparations contain the Herba Erodii extract of corilagin
Get suede back of the body Herba Erodii (Geranium vlassovianum) 2000g, pulverize, add 5 times of water gagings, heating decocts 2 times; Each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption the HPD400 50kg macroporous adsorptive resins of above-mentioned filtrating through having handled well.After upper prop was accomplished, resin column ended with 1 times of amount of resin 70% ethanol elution to elutriant terminal point with the water flushing of 8 times of amount of resin again, collects alcohol eluen.Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation again.Filtering and concentrating is to doing the Herba Erodii extract 33g that had both obtained containing corilagin, and the content of corilagin is 8% to use HPLC to detect wherein.
Embodiment 6 contains the influence of the Herba Erodii extract of corilagin to rat tolerance secretion
1 material
1.1 reagent
Herba Erodii extract makes according to the method for embodiment 2
Ranitidine hydrochloride Jiangxi Huiren Pharmaceutical Co., Ltd
Omeprazole AstraZeneca pharmaceutical Co. Ltd
Xylo-Mucine (CMC-Na) Chemical Reagent Co., Ltd., Sinopharm Group
Benzylpenicillin sodium for injection Huabei Pharmaceutic Co., Ltd
Chloral Hydrate Chemical Reagent Co., Ltd., Sinopharm Group
Sodium hydroxide Nanjing chemical reagent one factory
The Nereid analyses Chemical Industry Science Co., Ltd on the phenolphthalein
1.2 animal
Cleaning level SD rat, male, body weight 180-200g, Shanghai Slac Experimental Animal Co., Ltd..
1.3 instrument
Sartorius BS110S electronic balance Beijing Sai Duolisi balance ltd
LDZ5-2 whizzer Beijing Medical Centrifugal Machine Factory
1.4 group and drug dose setting
Model group CMC-Na (0.5%)
Ranitidine HCL group 100mg/kg
Omeprazole group 13.6mg/kg
Herba Erodii extract 200mg/kg
Herba Erodii extract 100mg/kg
1.5 medicine preparation and route of administration: medicine is with CMC-Na (0.5%) suspendible, gastric infusion, 10ml/kg.
2 methods
2.1 modeling method
Each organizes the rat oral gavage administration, and 1 time/d, continuous 5d.Fasting 48h before the experiment.During experiment with rat with Chloral Hydrate (0.30g/kg) anesthesia, carry out pyloric ligation, and duodenal administration 1 time, water is prohibited in the postoperative fasting, puts to death animal behind the 6h, collects gastric juice in graduated centrifuge tube.With 3000r/min, centrifugal 15min gets supernatant.
2.2 observation index
2.2.1 gastric juice amount
Behind the modeling 6h, put to death rat, collect gastric juice in graduated centrifuge tube.With 3000r/min, centrifugal 15min gets supernatant, accurately weighing gastric juice volume.
2.2.2 total acidity
Behind the modeling 6h, put to death rat, collect gastric juice in graduated centrifuge tube.With 3000r/min, centrifugal 15min gets supernatant, measures total acidity with 0.01mol/L NaOH through acid base neutralization titration (phenolphthalein is indicator), and calculates total acid output according to gastric secretion.
2.3 statistical procedures
Data are represented with X ± SD, carry out statistical procedures with SPSS11.0, relatively use the least significant difference method in twos between each group.
3. experimental result
Table 1. Herba Erodii extract is to pylorus ligation rat gastric juice excretory influence (X ± SD)
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
4 conclusions
Herba Erodii extract can reduce rat total acidity gastric juice and total acid output, and the prompting Herba Erodii extract has the effect of gastric acid inhibitory excretory, can be used to treat hyperchlorhydria, chronic gastritis.
Embodiment 7 contains the anti-gastric-ulcer experimentation on animals of the Herba Erodii extract of corilagin
1 experiment material:
1.1 animal:
The SD rat, male, 180-220g is provided by the Yangzhou University experimental center
1.2 medicine and reagent:
The Herba Erodii extract that contains corilagin: the method according to embodiment 2 makes
Positive drug: ranitidine hydrochloride capsules is provided by middle promise pharmaceutcal corporation, Ltd
Acetate: Shanghai is insulted wind chemical reagent ltd and is provided
Vetanarcol: Chinese Medicine Shanghai chemical reagents corporation provides
1.3 equipment:
The rat retaining plate, operating scissors, scalpel, tweezers, needle holder, microsyringe, balance
1.4 experiment condition:
The experimental rat sub-cage rearing is fed and is raised full-valence pellet feed, freely drinks water 20 ± 2 ℃ of room temperatures, humidity 50-70%
2. pyloric ligation ulcers rat gastric ulcer model
2.1 experimental technique
Rat is divided into 4 groups at random by body weight, 10 every group: the blank group, Ranitidine HCL group (135mg/kg) contains the Herba Erodii extract high dose group (150mg/kg) of corilagin, contains the Herba Erodii extract low dose group (75mg/kg) of corilagin.According to the continuous gastric infusion of 10ml/kg 5 days, the blank group gave equivalent zero(ppm) water to administration group rat respectively.Rat begins fasting and can't help water after the 1st administration, the last administration after 2 hours with rat with 0.7ml/ 1% shallow fiber crops of vetanarcol only, the ventrimeson open abdomen from the ensiform process of sternum lower edge, the about 2-3cm of otch.The costal margin position in the left side up one pushes away with referring to gently, makes stomach be exposed to otch.Under the stomach pylorus, wear a line (not injuring blood vessel) with pylorus ligation (other vascular ligations that will not be close to), sew up incision of abdominal wall, clean with saline water, iodine disinfection is with bundle cutting mouth.Fasting is put to death animal after prohibiting water 18h, opens the abdominal cavity, behind the ligation orifice of the stomach stomach is taken out.Collect gastric juice, volume calculated.Cut off stomach along greater gastric curvature,, be tiled on the blank sheet of paper, observe the situation that glandular stomach portion ulcer takes place, write down every group of ulcerogenic mouse number, ulcer number, ulcer level, ulcer area and pathology situation, calculate UI with saline water flush away gastric content.
The UI assessment method: the rat that gastric perforation do not occur is calculated UI with aphtha number and ulcer area.Calculate the area summation that all maximum diameters surpass the ulcer of 1mm, the area of each ulcer is according to S=π * r
1* r
2Calculate r
1And r
2Be respectively diameter and wide footpath; All count according to aphtha less than the ulcer of 1mm in diameter and wide footpath, with aphtha number and the ulcer total area as the Comprehensive Assessment index.Evaluation criteria sees the following form:
| UI | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
| The aphtha number | 0 | 1-5 | 6-30 | 31-60 | ＞60 | ||
| Ulcer area mm 2 | 0 | 1-10 | 11-20 | 21-30 | 31-40 | 41-50 | ＞50 or the perforation |
Ulcer suppresses percentage %=(blank group UI-medication group UI)/blank group UI * 100%
The ulcer incidence: the number of animals that ulcer takes place accounts for the per-cent of laboratory animal number.
2.2 experimental result
Herba Erodii extract is to the influence of pyloric ligation ulcers stomach ulcer (X ± s)
| Group | Dosage mg/kg | Number of animals | UI | Ulcer suppresses percentage % | Ulcer incidence % | Gastric juice volume ml |
| Blank | - | 10 | 4.0±1.7 | 100 | 17.8±3.0 | |
| Ranitidine HCL | 135 | 10 | 1.4±1.0 ** | 65 | 80 | 12.6±5.1 * |
| Herba Erodii extract | 150 | 10 | 0.9±0.9 ** | 77.5 | 50 | 9.7±3.4 ** |
| Herba Erodii extract | 75 | 10 | 2.3±1.0 * | 42.5 | 100 | 13.3±4.2 * |
Compare with the blank group
*Represent P＜0.05,
*Represent P＜0.01;
Above result shows: form tangible ulcer behind the rat pylorus ligation in this experiment, show the modeling success.Positive control medicine Ranitidine HCL can reduce gastric acid secretion, reduces the incidence and the UI of ulcer.The high and low dose that contains the Herba Erodii extract of corilagin can both reduce gastric acid secretion, reduces the incidence and the UI of ulcer.The Herba Erodii extract that contains corilagin reduces gastric acid secretion when high dosage, effects such as the incidence of reduction ulcer and UI are superior to the Ranitidine HCL group.
3 water logging irritability rat gastric ulcer models
3.1 experimental technique
Rat is divided into 4 groups at random by body weight, 10 every group: blank group, Ranitidine HCL group (135mg/kg), contain corilagin Herba Erodii extract high dose group (150mg/kg), contain the Herba Erodii extract low dose group (75mg/kg) of corilagin.Rat was pressed the continuous gastric infusion of 10ml/kg 5 days; The blank group gives equivalent zero(ppm) water, and last administration rat fasting in preceding 24 hours was fixed in the four limbs colligation of rat on the plank after the administration in 1 hour; Be dipped in the tank about 20 ℃, liquid level remains on the ensiform process of sternum level.Put to death rat after 20 hours, open the abdominal cavity, behind ligation orifice of the stomach and the pylorus stomach is taken out, in stomach, inject 1% formaldehyde solution 10ml, and stomach was immersed in 1% formaldehyde solution 10 minutes, fixedly ectonexine by pylorus.Cut off stomach along greater gastric curvature,, be tiled on the blank sheet of paper, observe the situation that glandular stomach portion ulcer takes place with saline water flush away gastric content.The degree of ulcer is represented with UI, and the incidence of calculating ulcer inhibition percentage and ulcer is estimated the effect of medicine.
UI: the major diameter of ulcer is directly measured length greater than 1mm person, and every 1mm is 1 minute; If then score greater than 1mm person and double in wide footpath, for major diameter and wide footpath all less than 1mm person, by the score of ulcer number, each ulcer meter 0.5 minute.The accumulative total total points of every animal is the UI of this animal.
Ulcer suppresses percentage %=(blank group UI-medication group UI)/blank group UI * 100%
The ulcer incidence: the number of animals that ulcer takes place accounts for the per-cent of experimental animal number.
3.2 experimental result
Herba Erodii extract is to the influence of water logging stress gastric ulcer model (X ± s)
Compare with the blank group
*Represent P＜0.05,
*Represent P＜0.01;
Experimental result shows: formed tangible stomach ulcer after the rat water logging in this experiment, shown the modeling success.Positive control medicine Ranitidine HCL has the obvious suppression effect to ulceration, and can significantly reduce UI.Close when containing the Herba Erodii extract high dosage of corilagin with the action effect of Ranitidine HCL, the ulceration of irritability rat gastric ulcer model is had restraining effect, and dose-effect relationship is obvious, the high dose group effect is superior to low dose group.
The 4 acetate type rat gastric ulcer model of burning
4.1 experimental technique
Rat is divided into 4 groups at random by body weight, 10 every group: blank group, Ranitidine HCL group (135mg/kg), contain corilagin Herba Erodii extract high dose group (150mg/kg), contain the Herba Erodii extract low dose group (75mg/kg) of corilagin.The rat fasting after 24 hours, is carried out intraperitoneal injection of anesthesia with 5mg/100g body weight vetanarcol, then its dorsal position is fixed on the operating table.Cut the stomach wall hair, use the alcohol routine disinfection, under xiphoid-process to the about 2cm of lower edge ventrimeson clip; Stomach is shifted out the abdominal cavity, at the facies ventralis of stomach, body of stomach and pyloric antrum intersection; Thrust 0.4-0.5mm under the serous coat with microsyringe is flat, inject the acetate of 50 μ l 10%, form papule.Stomach is sent back to gently, sew up abdominal muscle, skin suture then.With the tincture of iodine, alcohol disinfecting, the conventional raising.From performing the operation next day, each organizes every day by the 10ml/kg gastric infusion, successive administration 7 days, and the blank group is given equivalent zero(ppm) water.Animal was all put to death in the 7th day.Open fu jie and prick pylorus and orifice of the stomach, get stomach and be dipped in 1% formaldehyde solution, fixing 10 minutes.Cut off along greater gastric curvature, stomach is turned up, outwell content, wash out swill gently with the nipple suction pipe again.Ulcer is rounded or oval, and central concave is swelled all around slightly, and capillary vessel gathers.The ulcer that has healed has protuberance on every side slightly, and the surface is ruddy, it is thus clear that radial microgroove is arranged.Equal value representation UI with the major diameter of ulcer and minor axis carries out statistical procedures to calculate the ulcer healing percentage, and the evaluation medicine is to the promoter action of ulcer healing.
Ulcer healing percentage (%)=(blank group ulcer diameter average summation-medication group ulcer diameter average summation)/blank group ulcer diameter average summation * 100%
4.2 experimental result
To the burn influence (X ± s) of type stomach ulcer of rats acetic acid
Compare with the blank group
*Represent P＜0.05,
*Represent P＜0.01;
Healing to rat gastric ulcer when containing the Herba Erodii extract high dosage of corilagin has significant promoter action, can increase the ulcer healing percentage, reduces UI, and the effects such as incidence and UI that reduce ulcer are all near with the Ranitidine HCL winding.Little during low dosage to the influence of ulcer healing and UI.
5 conclusions
Through three kinds of ulcer experiments, the results suggest Herba Erodii extract that contains corilagin provided by the invention can be used to treat peptide ulceration.
Claims (7)
1. Herba Erodii extract, the per-cent that the corilagin that it is characterized in that this extract Chinese style I accounts for the extract total mass is 5%-40%, adopts following method preparation: it is some to get the Chinese medicine Herba Erodii, pulverizes; Add 5-10 times of water gaging, heating decocts 2-4 time, each 1.5 hours; United extraction liquid filters, and collects filtrating; With the macroporous adsorptive resins of above-mentioned filtrating, carry out dynamic adsorption, after upper prop is accomplished through having handled well; Resin column is eluted to the elutriant terminal point with the 1-4 times of arbitrary concentration ethanol of amount of resin 70-95% again and ends with the water flushing of 3-8 times of amount of resin, collects alcohol eluen; Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation, filtering and concentrating promptly obtains Herba Erodii extract to doing
2. the process for extracting of the described Herba Erodii extract of claim 1, it is some to comprise the steps: to get the Chinese medicine Herba Erodii, pulverizes, and adds 5-10 times of water gaging; Heating decocts 2-4 time, and each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption, after upper prop is accomplished the macroporous adsorptive resins of above-mentioned filtrating through having handled well; Resin column is eluted to the elutriant terminal point with the 1-4 times of arbitrary concentration ethanol of amount of resin 70-95% again and ends with the water flushing of 3-8 times of amount of resin, collects alcohol eluen; Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% ethanol sedimentation, filtering and concentrating promptly gets Herba Erodii extract to doing.
3. the process for extracting of the described Herba Erodii extract of claim 2, wherein the model of macroporous adsorptive resins is selected from HPD100, HPD400, HPD600.
4. the process for extracting of the described Herba Erodii extract of claim 3, wherein the model of macroporous adsorptive resins is HPD400.
5. the process for extracting of the described Herba Erodii extract of claim 2, it is some to comprise the steps: to get the Chinese medicine Herba Erodii, pulverizes, and adds 7 times of water gagings; Heating decocts 2 times, and each 1.5 hours, united extraction liquid filtered; Collect filtrating,, carry out dynamic adsorption, after upper prop is accomplished the HPD400 macroporous adsorptive resins of above-mentioned filtrating through having handled well; Resin column ends with 2 times of amount of resin 95% ethanol elution to elutriant terminal points with the water flushing of 5 times of amount of resin again, collects alcohol eluen; Alcohol eluen partly is evaporated to dried, spends the night with an amount of 95% dissolve with ethanol, filtering and concentrating promptly gets Herba Erodii extract to doing.
6. the described Herba Erodii extract of claim 1 is in the secretion of preparation gastric acid inhibitory, the purposes in the medicine of reduction gastric acidity.
7. the purposes of the described Herba Erodii extract of claim 1 in the medicine of preparation treatment or prevention hyperchlorhydria, peptide ulceration, chronic gastritis.
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Non-Patent Citations (2)
| Title |
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| 李克明等.不同采收月份老鹳草生药中柯里拉京的含量比较.《中成药》.2004,第26卷(第10期), * |
| 范红艳等.老鹳草提取物对消炎痛型及利血平型胃溃疡小鼠溃疡指数的影响.《延边大学医学学报》.2006,第29卷(第4期), * |
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