CN101603896A - Analytic system and analytical approach thereof, flow passage structure - Google Patents
Analytic system and analytical approach thereof, flow passage structure Download PDFInfo
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- CN101603896A CN101603896A CNA2008101099292A CN200810109929A CN101603896A CN 101603896 A CN101603896 A CN 101603896A CN A2008101099292 A CNA2008101099292 A CN A2008101099292A CN 200810109929 A CN200810109929 A CN 200810109929A CN 101603896 A CN101603896 A CN 101603896A
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Abstract
The invention discloses a kind of analytic system and analytical approach thereof, flow passage structure.This analytic system comprises a working fluid, an equal subdivision and a separative element.Working fluid has comprised having one first composition of different nature and one second composition.All subdivision can rotate with respect to an axis of reference, and all subdivision is divided equally for working fluid.Under the acting in conjunction of the composite force of capillary force, Ke Shi power and siphon power, separative element system can separate between first composition of equal subdivisions and second composition for the position.
Description
Technical field
The present invention is relevant for a kind of flow passage structure, and particularly (for example: an inertial force that coriolis acceleration) is produced (for example: Ke Shi power) and analytic system of separating between the heterogeneity of different nature for having in the tested sample and analytical approach thereof, flow passage structure for the inertia phenomena of turning relevant for utilizing fluid.
Background technology
Its structure of known fluid separation device is comparatively complicated usually, and for example the disclosed fluid separation device of U.S. Pat 6548788 has the design of little valve, in order to flowing of control fluid on its card casket.But because little valve must be made with micro-processing technology, therefore known fluid separation device also can't be made through the mode of plastics ejection formation, thereby cost is higher.
Same, U.S. Pat 5061381 and US5089417 have also disclosed the fluid separation device of other form, and its structure is too complicated, so manufacturing cost is higher.
Summary of the invention
In view of this, the invention provides a kind of flow passage structure, be applicable to for having between one first composition of different nature and one second composition in the tested sample and carry out centrifugal the separation.Flow passage structure of the present invention comprises one first room, one second room, one the 3rd room and one the 4th room.
Second room is connected in first room and can rotates with respect to an axis of reference, and when tested sample was arranged at first room, tested sample transferred to second room.The 3rd room is connected in second room.The 3rd room has comprised interconnective one first buffer zone and one second buffer zone.When second room with respect to axis of reference and when a first direction rotates, the position is delivered to the 3rd room at the tested sample of second room at one first given time, and separating between the position produces tested sample at one second given time at the tested sample of first buffer zone of the 3rd room first composition and second composition, wherein, second given time is later than first given time, and second buffer zone of the 3rd room has been full of the first separated composition.The 4th room is connected in the 3rd room, when second room stop operating with respect to axis of reference and the quiet special time that stagnates after, the position is delivered to the 4th room at first composition of the tested sample of second buffer zone of the 3rd room, and when second room from stationary state and and when a second direction that is different from first direction is rotated with respect to axis of reference, outwards transmit via the 4th room by an acting force at the first separated composition of the 4th room the position, so makes the composition of winning be located away from second composition fully.
Flow passage structure more can comprise one the 5th room.The 5th room is connected in the 4th room, when second room from stationary state and with respect to axis of reference when second direction is rotated, the position transfers to the 5th room at the first separated composition of the 4th room by an acting force, so makes the composition of winning be located away from second composition fully.
Flow passage structure more can comprise one first channel.First channel is connected between first room and second room.
Flow passage structure more can comprise one second channel.Second channel is arranged between second room and the 3rd room.Second channel is with respect to axis of reference and along a radially-arranged capillary channel.When second room stopped operating with respect to axis of reference, the position automatically was delivered to the 4th room in the first components utilising capillary action of the tested sample of second buffer zone of the 3rd room.Second buffer zone of the 3rd room comprises a line style capillary channel.The 4th room comprises a line style capillary channel.Have one first angle between first buffer zone of the 3rd room and second buffer zone, this angle system is not more than 30 degree.Have one second angle between second buffer zone of the 3rd room and the 4th room, this angle system is not less than 90 degree.
Flow passage structure more can comprise a turnover channel.Turnover channel position is between second buffer zone and the 4th room of the 3rd room.First buffer zone of second room and the 3rd room is with respect to axis of reference and along radial distribution.
Flow passage structure more can comprise one the 6th room.The 6th room is connected in the 3rd room and the 5th room.
Flow passage structure more can comprise the main body with a basal plane.First buffer zone of first room, second room, the 3rd room and second buffer zone, the 4th room are the multi-groove structure on the basal plane that is formed at main body jointly.Second buffer zone of the 3rd room and the groove constructional depth of the 4th room are less than the groove constructional depth of first buffer zone of first room and the 3rd room.First buffer zone of the 3rd room has comprised an interconnective first area, a second area and a zone line, the first area is connected in second room, the zone line position is between first area and second area, and between first area and the zone line, have channel degree of depth offset respectively between second area and the zone line, second buffer zone is connected in zone line.
Acting force has comprised making a concerted effort of the Ke Shi power that produced by a coriolis acceleration and centrifugal force.Tested sample is subjected to respect to the effect of an accelerated motion of axis of reference at first given time and moves.Tested sample is subjected to respect to the effect of a constant speed of axis of reference motion and moves at second given time.The proportion of first composition is different from the proportion of second composition.
In addition, the invention provides a kind of analytic system, this analytic system comprises a working fluid, an equal subdivision, a separative element and an eccentric groove.
Working fluid has comprised having one first composition of different nature and one second composition.All subdivision can rotate with respect to an axis of reference, divides equally for working fluid by equal subdivision.Separative element is in order to carry out centrifugal the separation between first composition of position in the working fluid of equal subdivisions and second composition.Separative element comprises that an eccentric groove separates channel with one.Eccentric groove has comprised interconnective one first buffer zone and one second buffer zone, when equal subdivision with respect to axis of reference and when a first direction rotates, the position is delivered to eccentric groove at the working fluid of equal subdivision at one first given time, and separating between the position produces working fluid at one second given time at first buffer zone of eccentric groove and the working fluid of second buffer zone first composition and second composition, wherein, second given time is later than first given time, and second buffer zone of eccentric groove has been full of first composition of working fluid.Separate channel and be connected in eccentric groove.When equal subdivision stop operating with respect to axis of reference and the quiet special time that stagnates after, the position is delivered to first composition of the working fluid of second buffer zone at first buffer zone of eccentric groove separates channel, and when equal subdivision from stationary state and and when a second direction that is different from first direction is rotated with respect to axis of reference, the position via separating the centrifugal outside transmission of channel, so makes the composition of winning be located away from second composition fully by an acting force at first composition of the working fluid that separates channel.
Separative element has also comprised a detecting groove.The detecting groove is connected in the separation channel, when equal subdivision from stationary state and with respect to axis of reference when second direction is rotated, the position transfers to the detecting groove at first composition of the working fluid that separates channel by an acting force, so makes the composition of winning be located away from second composition fully.The proportion of first composition is different from the proportion of second composition.Working fluid comprises a blood, and first composition comprises blood plasma, and second composition comprises blood cell.
Separative element also comprises one second channel.Second channel is arranged between equal subdivision and the eccentric groove.Second channel is with respect to axis of reference and along radial distribution.Second channel is a capillary channel.When equal subdivision stopped operating with respect to axis of reference, the position automatically was delivered to the separation channel in the first components utilising capillary action of the working fluid of second buffer zone of eccentric groove.
Second buffer zone of eccentric groove comprises a line style capillary channel.Separate channel and comprise a line style capillary channel.Have one first angle between first buffer zone of eccentric groove and second buffer zone, this angle is not more than 30 degree.Second buffer zone of eccentric groove has one second angle with separating between the channel, this angle is not less than 90 degree
Separative element also comprises a turnover channel, turnover channel position second buffer zone of eccentric groove with separate between the channel.All first buffer zone of subdivision and eccentric groove is with respect to axis of reference and along radial distribution.
Separative element also comprises an air discharge duct.Air discharge duct is connected in eccentric groove and separates channel.
Analytic system more can comprise the main body with a basal plane.All subdivision and first buffer zone of the eccentric groove of separative element with second buffer zone, separate channel and be the multi-groove structure on the basal plane that is formed at main body jointly.
Second buffer zone of eccentric groove and the groove constructional depth of the groove constructional depth of separating channel much smaller than first buffer zone of eccentric groove.
First buffer zone of eccentric groove has comprised an interconnective first area, a second area and a zone line, first area system is connected in equal subdivision, the zone line position is between first area and second area, and between first area and the zone line, have channel degree of depth offset respectively between second area and the zone line, second buffer zone is connected in zone line.
Acting force has comprised a Ke Shi power and a centrifugal force that is produced by a coriolis acceleration.Working fluid is subjected to respect to the effect of an accelerated motion of axis of reference at first given time and moves.Working fluid is subjected to respect to the effect of a constant speed of axis of reference motion and moves at second given time.
Analytic system more can comprise having one first a plurality of objects of demarcating thing, and a plurality of objects are arranged among the equal subdivision, and tested sample comprises that also one second demarcates thing, second of tested sample demarcate between the first demarcation thing of thing and a plurality of objects can in conjunction with.
A plurality of objects comprise carriers such as glass spheres, magnetic bead.First demarcates thing has comprised nucleic acid, protein, biomarker molecule, antibody, cell or other biomolecule with joint.Second demarcate nucleic acid that thing comprised the complementation of tool mark function, be subjected to matter, ferment, coenzyme, complement, antigen, other cell or biomolecule.
Again, the invention provides a kind of analytical approach, this analytical approach may further comprise the steps: provide to have comprised the working fluid with one first composition of different nature and one second composition; Provide an equal subdivision to rotate with respect to an axis of reference, all subdivision is divided equally for working fluid; And provide an eccentric groove to separate channel with one, eccentric groove with separate channel and carry out centrifugal the separation between first composition of position in the working fluid of equal subdivisions and second composition in order, wherein, eccentric groove has comprised interconnective one first buffer zone and one second buffer zone.
When equal subdivision with respect to axis of reference and when a first direction rotates, the position is delivered to eccentric groove at the working fluid of equal subdivision at one first given time, and separating between the position produces working fluid at one second given time at first buffer zone of eccentric groove and the working fluid of second buffer zone first composition and second composition, wherein, second given time is later than first given time, and second buffer zone of eccentric groove has been full of first composition of working fluid; Separate channel and be connected in eccentric groove, when equal subdivision stop operating with respect to axis of reference and the quiet given time that stagnates after, the position is delivered to first composition of the working fluid of second buffer zone at first buffer zone of eccentric groove separates channel, and when equal subdivision from stationary state and and when a second direction that is different from first direction is rotated with respect to axis of reference, the position via separating the centrifugal outside transmission of channel, so makes the composition of winning be located away from second composition fully by an acting force at first composition of the working fluid that separates channel.
Analytical approach more provides a detecting groove.The detecting groove is connected in the separation channel, when equal subdivision from stationary state and and when second direction is rotated with respect to axis of reference, the position transfers to the detecting groove at first composition of the working fluid that separates channel by an acting force, so makes the composition of winning be located away from second composition fully.
Analytical approach more provides one second channel.Second channel is arranged between equal subdivision and the eccentric groove.Second channel is with respect to axis of reference and along radial distribution.When equal subdivision stopped operating with respect to axis of reference, the position automatically was delivered to the separation channel in the first components utilising capillary action of the working fluid of second buffer zone of eccentric groove.One first angle is provided between first buffer zone of eccentric groove and second buffer zone, and this angle is not more than 30 degree.Second buffer zone of eccentric groove provides one second angle with separating between the channel, this angle is not less than 90 degree.
Analytical approach more provides a turnover channel.Turnover channel position second buffer zone of eccentric groove with separate between the channel.Acting force has comprised a Ke Shi power and a centrifugal force that is produced by a coriolis acceleration.Working fluid is subjected to respect to the effect of an accelerated motion of axis of reference at first given time and moves.Working fluid is subjected to respect to the effect of a constant speed of axis of reference motion and moves at second given time.
Analytical approach more provides a plurality of objects with one first demarcation thing.A plurality of objects are arranged among the equal subdivision, and tested sample comprises that also one second demarcates thing, second of tested sample demarcate between the first demarcation thing of thing and a plurality of objects can in conjunction with.First demarcates thing has comprised nucleic acid, protein, biomarker molecule, antibody, cell or other biomolecule with joint.Second demarcate nucleic acid that thing comprised the complementation of tool mark function, be subjected to matter, ferment, coenzyme, complement, antigen, other cell or biomolecule.The proportion of first composition is different from the proportion of second composition.
For above and other objects of the present invention, feature and advantage can be become apparent, a preferred embodiment cited below particularly, and cooperate appended diagram, be described in detail below:
Description of drawings
Figure 1A is the combination stereogram of an expression flow passage structure of the present invention;
Figure 1B is the exploded perspective view of an expression flow passage structure of the present invention;
Fig. 2 A is the stereographic map of the main body of the flow passage structure in the presentation graphs 1;
Fig. 2 B is that the partial enlarged drawing in the single stream district of the flow passage structure in the presentation graphs 1 shows;
Fig. 2 C is another embodiment of the main body of expression flow passage structure of the present invention;
Fig. 3 is that the partial enlarged drawing in the single stream district of the flow passage structure in the zone (Y) of presentation graphs 2B shows;
Fig. 4 is the operational flowchart of expression analytic system of the present invention;
Fig. 5 A is that expression is with the synoptic diagram of a tested sample delivery to first room in single stream district;
Fig. 5 B is that expression is divided equally the synoptic diagram that the rear section flow to second room according to the tested sample that is positioned at first room among Fig. 5 A;
Fig. 5 C be expression when main body with respect to an axis of reference (a1-a1) and the synoptic diagram after a first direction (N1) rotates;
Fig. 5 D be expression when the main body among Fig. 5 C stop operating and a quiet stagnant special time after, the first separated composition is transported to the synoptic diagram of the 4th room;
Fig. 5 E be the main body of expression in Fig. 5 D from stationary state and with respect to axis of reference (a1-a1) and along a second direction (N2) rotate, the first separated composition transfers to the synoptic diagram in the 5th room fully via the 4th room; And
Fig. 6 A～6C be shown in the analytic system of the present invention the synoptic diagram of the biochemical reaction of being correlated with and optical detection.
[primary clustering symbol description]
A1-a1～axis of reference
B0～filling orifice
B1～main body
B1～stream district
B1 '～main body
B100～basal plane
B2～upper cover body
B3～lower cover
BIO-CO～bioplex
C1～first room
C2～second room
C3～the 3rd room
C3 '～the 3rd room
C30i～first area
C30j～second area
C30j～second area
C30k～merging zone
C30o～zone line
C3-1～first buffer zone
C3-1 '～first buffer zone
C3-2～second buffer zone
C4～the 4th room
C5～the 5th room
C6～the 6th room
Fc～acting force
Hi, h, hj～degree of depth
Hi-h～offset
Hj-h～offset
K～working fluid
K01～first composition
K02～second composition
L～luminous product
M～flow passage structure
MOL～demarcation molecule
N1～first direction
N100, n102, n104～step
Non-TA～non-detection target
N2～second direction
Q～object
S1～dip plane
S2～vertical plane
SUB～be subjected to matter
T～sampling thief
T1～first given time
T2～second given time
V1～first channel
V2～second channel
V3～turnover channel
V4～exhaust channel
V5～exhaust channel
W1～equal subdivision
W2～separative element
W3～exhaust unit
W4～detecting unit
X1～radially
Y～zone
Z～analytic system
θ～first angle
α～second angle
Embodiment
Figure 1A is the combination stereogram of an expression flow passage structure M of the present invention, and Figure 1B is the exploded perspective view of an expression flow passage structure M of the present invention.
Flow passage structure M comprises a main body B1, upper cover body B2 and a lower cover B3.Main body B1 have flow path designs and be arranged at upper cover body B2 and lower cover B3 between, and utilize upper cover body B2 to cover, so to form a closed structure for the stream that is arranged at main body B1.In the present embodiment, upper cover body B2 is a film, and this film utilizes packaged type and is incorporated on the main body B1.The mutual collocation effect of flow passage structure M of the present invention by capillary force, centrifugal force and the composite force of siphon power down and can (for example: multicomponent working fluid proportion) (for example: the tested sample of a blood or a biochemical corpse or other object for laboratory examination and chemical testing) divide equally and separate, so to reach the purpose of analyzing with detecting for having heterogeneity.
Fig. 2 A, 2B are the stereographic maps of the main body B1 among expression Figure 1B.
Shown in Fig. 2 A, 2B, main body B1 comprises a basal plane b100, a filling orifice b0 and multithread road district b1.Filling orifice b0 and multithread road district b1 are arranged on the basal plane b100 jointly, and filling orifice b0 is communicated to a plurality of streams district b1 respectively.One axis of reference a1-a1 defines the position of filling orifice b0, and the configuration of a plurality of streams district b1 with axis of reference a1-a1 be the center and respectively along a plurality of radially X1 carry out uniformly-spaced and the symmetry radial distribution.Main body B1 can rotate along a first direction N1 or a second direction N2 with respect to axis of reference a1-a1.
Have identical construction though it should be noted that each stream district b1 in the present embodiment, and the number of stream district b1 is four.Yet, though the filling orifice b0 in the present embodiment and a plurality of streams district b1 are arranged on the same plane (basal plane b100) jointly, but the position of filling orifice b0 and stream district b1 and number thereof are not in order to restriction the present invention, can utilize reach divide equally with the effect that separates under, suitable change and retouching can be done by the number of filling orifice b0 and stream district b1 and the position at place thereof system.Below narration will describe with single stream district b1.
Stream district b1 comprises one first room C1, one second room C2, one the 3rd room C3, one the 4th room C4, one the 5th room C5, one the 6th room C6, one first channel V1 (shown in 2B figure), one second channel V2, a turnover channel V3 and two exhaust channel V4/V5, wherein, the first room C1, the second room C2, the 3rd room C3, the 4th room C4, the 5th room C5, the 6th room C6, the first channel V1, the second channel V2, turnover channel V3, two exhaust channel V4/V5 are the multi-groove structure on the basal plane b100 that is formed at main body B1 jointly.
In one embodiment, removed upper cover body B2 earlier before bringing into use this analytic system, win room C1 and the 6th room C6 are connected with ambient atmosphere, wherein, the first room C1 is the sample filling orifice, and the 6th room C6 is a vent port.
With regard to the flow path designs function of the main body B1 of flow passage structure M, each stream district b1 mainly can divide into an equal subdivision W1, a separative element W2, an exhaust unit W3 and a detecting unit W4.
All subdivision W1 comprises the first room C1, the second room C2, the first channel V1 and the second channel V2.
In one embodiment, the first room C1 is an endless groove structure that is arranged on the basal plane b100, by the first room C1 to have constituted the accommodation space of filling orifice b0.The second room C2 divides equally groove to distribute and to be arranged at one on the basal plane b100 with respect to axis of reference a1-a1 and along X1 radially.The first channel V1 is a hollow bulb that is arranged between the bottom of the bottom of the first room C1 and the second room C2, thus with for connecting between the first room C1, the second room C2.The second channel V2 ends to valve or capillary valves as one by the second channel V2 for being arranged at basal plane b100 and being connected in a linear pattern capillary channel or the groove structure of the second room C2 along X1 radially with respect to axis of reference a1-a1.In other words, the degree of depth of the second channel V2 is much smaller than the degree of depth of the second room C2.
Separative element W2 comprises the 3rd room C3, turnover channel V3, the 4th room C4.
The 3rd room C3 is for substantially being arranged at a eccentric groove on the basal plane b100 with respect to axis of reference a1-a1 and along X1 radially, and the side of the 3rd room C3 is connected in the second channel V2 of equal subdivision W1, that is the second channel V2 is arranged between the second room C2 and the eccentric groove C3.The 3rd room C3 has comprised interconnective one first buffer zone c3-1 and one second buffer zone c3-2.The first buffer zone c3-1 has comprised the straight type groove structure of an interconnective first area c30i, a zone line c30o and a second area c30j, and under with respect to basal plane b100.
See also Fig. 2 B and Fig. 3, first area c30i, zone line c30o and the second area c30j of the first buffer zone c3-1 of the 3rd room C3 have degree of depth hi, h and hj respectively, wherein, have an offset hi-h between the degree of depth h of the degree of depth hi of first area c30i and zone line c30o, have another offset hj-h between the degree of depth h of the degree of depth hj of second area c30j and zone line c30o.First area c30i has constituted the Upstream section of eccentric groove and has been connected in the second channel V2, and second area c30j has constituted the tract of eccentric groove, and zone line c30o position is between first area c30i and second area c30j and be connected in the second buffer zone c3-2.Wherein, under with respect to basal plane b100, between first area c30i and zone line c30o, have a dip plane S1, between second area c30j and zone line c30o, have a vertical plane S2.
The second buffer zone c3-2 of the 3rd room C3 is a linear pattern capillary channel that is arranged at basal plane b100 and is connected in the zone line c30o of the first buffer zone c3-1.In other words, the channel degree of depth of the second buffer zone c3-2 is much smaller than degree of depth hi or the degree of depth h of zone line c30o or the degree of depth hj of second area c30j of first area c30i.It should be noted that between the first buffer zone c3-1 of the 3rd room C3 and the channel bearing of trend of the second buffer zone c3-2 (that is, radially X1) to have one first angle theta, this angle theta system is to be not more than 30 degree for good.In the present embodiment, the design of first angle theta is about 23 degree.When the first room C1 injects liquid, can increase the success ratio that liquid is stopped by capillary valves by main body B1.
See also Fig. 2 C, Fig. 2 C is the synoptic diagram of another Embodiment B 1 ' of expression main body B 1.Be with interconnective first area c30i, zone line c30o among the first buffer zone c3-1 of main body B1 and the trizonal main difference of second area c30j: the straight type groove structure of the first buffer zone c3-1 ' among the main body B1 ' can be designed to interconnective two zones according to demand, that is, first area c30i in the foregoing description and zone line c30o merging are become the regional c30k of a merging with degree of depth h, merge the first buffer zone c3-1 ' that regional c30k and second area c30j have formed the 3rd room C3 ' of main body B1 ' thus.When the first room C1 injects liquid, can increase the success ratio that liquid is stopped by capillary valves equally by main body B1 '.
Turnover channel V3 is arranged on the basal plane b100 and is connected in the second buffer zone c3-2 of the 3rd room C3 and a V-type shape capillary channel or the groove structure between the 4th room C4.The 4th room C4 is that a linear pattern capillary that is arranged on the basal plane b100 is separated channel or groove structure.In other words, turnover channel V3 and the 4th room C4 are continuously and can have the capillary channel or the groove structure of same depth, but the degree of depth of the degree of depth of turnover channel V3 and the 4th room C4 is much smaller than degree of depth hi or the degree of depth h of zone line c30o or the degree of depth hj of second area c30j of first area c30i.It should be noted that between the bearing of trend of the 4th room C4 and the second buffer zone c3-2 to have one second angle α, this angle α is to be not less than 90 degree for good.In present embodiment, the design of the second angle α is about 95 degree.
Based on above-mentioned explanation as can be known, the second buffer zone c3-2 of the 3rd room C3, turnover channel V3 and the 4th room C4 have constituted continuously and the capillary channel that can have same depth jointly, wherein, the second buffer zone c3-2 of the 3rd room (eccentric groove) C3 has constituted the Upstream section of this continuous capillary channel, and the 4th room (separation channel) C4 has constituted the tract of this continuous capillary channel.
Please consult the 3rd figure simultaneously, the 3rd figure is that the partial enlarged drawing of the single stream district b1 of the flow passage structure M among the regional Y of expression 2B figure shows.
Detecting unit W4 comprises a plurality of object Q and the 5th room C5 with first demarcation thing.A plurality of object Q be arranged in alternative mode the second room C2 in.The 5th room C5 is a cylindric detecting groove that is arranged on the basal plane b100 and is connected in the 4th room C4.In the present embodiment, a plurality of object Q are the glass spheres (or claim glass microballoon) of particle diameter between 200～1000 microns (μ m), have first and demarcate thing on a plurality of object Q, this first demarcation thing can be nucleic acid (DNA or RNA), protein (protein), biomarker molecule (biomarker), antibody (antibody), cell (cell) or other biomolecule with joint.It should be noted that in addition, glass spheres is utilized processing procedure (containing physics or chemical method) before one step or the multiple step and can be reached and catch the function of getting specific objective, wherein, handle and form one on the surface of glass spheres through physics mode (for example: high temperature heating, absorption or deposition) and coat thin layer, or (for example: aminated (HH2), hydroxyization (OH), shuttle baseization (COOH), aldehyde radicalization (CHO) etc.) and produce and coat thin layer on the glass microballoon surface can handle via chemical mode.In addition, in other embodiments, detecting unit can be included in separative element in, a plurality of objects also can be magnetic bead, entity carrier or other has the structure of identical function.
Exhaust unit W3 comprises exhaust channel V4, the 6th room C6 and exhaust channel V5.Exhaust channel V4 is a linear pattern capillary channel or the groove structure that is arranged on the basal plane b100 and is connected in the 5th room C5.The 6th room C6 is a cylindric air discharge duct that is arranged on the basal plane b100 and is connected in exhaust channel V4.Exhaust channel V5 is a linear pattern capillary channel or the groove structure that is arranged on the basal plane b100, this exhaust channel V5 is connected between the first area c30i of the first buffer zone c3-1 of the 6th room C6 and the 3rd room C3, if the embodiment of Fig. 2 C, this exhaust channel V5 are connected between the merging zone c30k of the first buffer zone c3-1 of the 6th room C6 and the 3rd room C3.In other words, the degree of depth of exhaust channel V4/V5 is much smaller than the degree of depth of the 5th room C5 or the degree of depth of the 6th room C6.
Fig. 4 is the operational flowchart of expression analytic system Z of the present invention.Analytical approach of the present invention comprises that following key step: step n100 provides and has comprised the working fluid K with one first ingredient k 01 of different nature and one second ingredient k 02; Step n102 provides an equal subdivision W1, and this equal subdivision W1 can rotate with respect to an axis of reference a1-a1, and all subdivision W1 divides equally for working fluid K; And step n104 provides and has an eccentric groove C3 separates channel C4 with one separative element W2, under the acting in conjunction that cooperates by the composite force of capillary force, Ke Shi power, centrifugal force and siphon power, the eccentric groove C3 that utilizes separative element W2 with separate channel C4 and carry out centrifugal the separation between first ingredient k 01 of position in the working fluid K of equal subdivision W1 and second ingredient k 02 in order.
Below will cooperate Fig. 5 A～5F and represent the synoptic diagram of analytic system Z when operation respectively.
Fig. 5 A is that expression utilizes a sampling thief (for example: suction pipe (pipette)) point (tip) of T will have both constant volumes (for example: tested sample K 50ul) is delivered to the synoptic diagram of one first room C1 of the filling orifice b0 of flow passage structure M, and Fig. 5 B is that expression is divided equally to the synoptic diagram of each second room C2 at the tested sample K of the first room C1 according to the position among Fig. 5 A.Tested blood sample K has one first ingredient k 01 and one second ingredient k 02 of different in kind, and for example: the proportion of first ingredient k 01 is less than the proportion of second ingredient k 02.In the present embodiment, tested sample K is a blood, and first ingredient k 01 is blood plasma (plasma), and second ingredient k 02 is blood cell (blood cells), and the proportion of blood plasma is less than the proportion of blood cell.In the embodiment of 5A figure, a plurality of object Q select to be arranged at equal subdivision W1 the second room C2 in, so to measure for tested sample K.
When the tested sample K when the position in sampling thief T is arranged at the first room C1 via filling orifice b0, because the restriction of the capillary structure of the second channel V2, tested sample K only can riddle each first channel V1, each second room C2 of equal subdivision W1 and each second channel V2 in, that is, tested sample K is divided equally to each second room C2, and tested sample K can not enter among the 3rd room C3 of separative element W2.
It should be noted that, injecting the previous crops sample just quantitatively and pick the use except using suction pipe to inject tested sample and being convenient to, also can use kapillary (not shown) Direct Sampling, adopting the full back test piece central authorities that insert, can utilize because of hydrophilic film concerns automatic transport at liquid capillaceous and extremely respectively divide equally groove.
Tested sample K comprises that one second demarcates thing, and this second demarcates nucleic acid that thing can be the complementation of tool mark function, is subjected to matter (substrate), ferment (enzyme), coenzyme (coenzyme), complement (complement), antigen (antigen), other cell or biomolecule.When the position in the second room C2 tested sample K and after a plurality of object Q reaction left standstill a given time, second of tested sample K demarcates thing can be by the contact of object to be measured (Target), can and first the demarcating between the thing and mutually combine of a plurality of object Q (that is a biological complex BIO-CO, shown in Fig. 6 B).
Fig. 5 C be expression when main body B1 with respect to axis of reference a1-a1 and the synoptic diagram after first direction N1 rotates.
The position can fully be delivered to the first buffer zone c3-1 and the second buffer zone c3-2 of each the 3rd room C3 in one first given time t1 at the tested sample K of each second room C2, and separating between the position produces tested sample K at one second given time t2 at the tested sample K of the first buffer zone c3-1 of the 3rd room C3 and the second buffer zone c3-2 first ingredient k 01 and second ingredient k 02, wherein, the second given time t2 is later than the first given time t1, and the second buffer zone c3-2 of the 3rd room C3 has been full of the first separated ingredient k 01.In the present embodiment, first direction N1 is a counterclockwise, rotative speed is set at per minute 4000 changes (RPM), and the rotation of the first given time t1 has comprised the accelerated motion (time: 0～5 second) (rotating speed: by 0 to 4000RPM) at a phase one (initial stage), and the second given time t2 has comprised the movement at the uniform velocity (time: 5～60 seconds) (rotating speed: maintain 4000RPM) in a subordinate phase (later stage).
When the accelerated motion of carrying out the phase one (first given time t1:0～5 second) (rotating speed: by 0 to 4000RPM), owing to making the position break through the first buffer zone c3-1 and the second buffer zone c3-2 that the second channel V2 enters the 3rd room C3 except meeting under the centrifugal action of high speed rotating at the tested sample K of each second room C2.
When the movement at the uniform velocity of carrying out subordinate phase (second given time t2:5～60 second) (rotating speed: in the time of 4000RPM), because the proportion of first ingredient k 01 is less than the proportion of second ingredient k 02, under the centrifugal action of rotation, just make second ingredient k 02 be stuck in the bottom side of second area c30j of the first buffer zone c3-1 of the 3rd room C3 with larger specific gravity, separated first ingredient k 01 with less proportion then is top side and the zone line c30o of second area c30j that is stuck in the first buffer zone c3-1 of the 3rd room C3, and centrifugal force also makes the first separated ingredient k 01 stop on the position of turnover channel V3.
See also 5D figure.5D figure be expression when the main body B1 among the 5C figure stop operating and a quiet stagnant special time after, separated first ingredient k 01 is delivered to the synoptic diagram of the 4th room C4 because of capillarity.When main body B 1 stop operating and the quiet special time that stagnates after, because the capillary action that the second buffer zone c3-2, the 4th room C4 of the 3rd room C3 and position are produced between the first separated ingredient k 01 of turnover channel V3 so makes the first separated ingredient k 01 be sent to the 4th room C4 by turnover channel V3.
See also 5E figure.5E figure be expression when the main body B1 among the 5D figure from stationary state and with respect to axis of reference a1-a1 and the synoptic diagram when a second direction N2 who is different from first direction N1 slowly runs.In the present embodiment, second direction N2 is one clockwise.
When main body B1 carries out than low velocity (about 2000～2500RPM from stationary state and along second direction N2,5～15 seconds) rotation the time, then the position the first separated ingredient k 01 of the 4th room C4 can a directed force F c[that is, by the composite force of Ke Shi power (with making a concerted effort of relative centrifugal force(RCF) difference) with siphon power] and outwards transmit via the 4th room C4, so make the ingredient k 01 of winning be located away from second ingredient k 02 fully and filled up the 5th room C5 of detecting unit W4, and the position can be reacted with the reagent (not shown) that is preset among the 5th room C5 in first ingredient k 01 of the 5th room C5.
In another embodiment, when nothing presets reaction reagent in the 5th room C5, then first ingredient k 01 is located away from second ingredient k 02 fully and has filled up the 5th room C5 of detecting unit W4, that is only in addition native system finish detached job.Whether the k01 color that observe the 5th room this moment presents transparent light yellow look, whether make blood to be measured (tested sample K) has the inspection of haemolysis, as present redness, this blood sample to be measured lost efficacy (in the blood just to be measured haemolysis being arranged) then, and be not suitable for being used as the sample that the card casket detects again, the user gets resampling.
Exception be it should be noted that, compared to axis of reference a1-a1 other the groove structure the position as can be known, the 5th room C5 position of detecting unit W4 has on the position of the maximum turning radius, so can improve the stability of reagent at the 5th room C5 of detecting unit W4.Above-mentioned when dividing equally with detached job when carrying out, the exhaust channel V4 of exhaust unit W3, the 6th room C6 and exhaust channel V5 discharge for the gas in each groove structure, so with convenient carrying out of dividing equally with detached job.In addition, all subdivision W1 and separative element W2 are when dividing equally with detached job, and the exhaust operation all carrying out among subdivision W1 and the separative element W2, so could make carry out smoothly dividing equally with detached job of equal subdivision W1 and separative element W2 simultaneously.
Comprehensive above-mentioned explanation below is briefly bright at proposing on the related application of analytic system Z of the present invention and flow passage structure M thereof.
Shown in Fig. 5 B, when the tested sample K (blood) among the second room C2 (dividing equally groove) of position at flow passage structure M after sufficient standing reaction a period of time, a plurality of object Q (glass microballoon) in the second room C2 (dividing equally groove) can interosculate with target to be measured and with the second level biomolecule with mark function.If during the fluoroscopic examination mode, first order biomolecule sees through target molecule to be measured and can mutually combine with the second level biomolecule that has illuminophore, and reads fluorescence signal in the 5th room C5 (detecting groove).And if cold light or light absorbing detection are when surveying mode, first order biomolecule sees through detected object and with after second level biomolecule combines, in the 5th room C5 (detecting groove), can produce reaction, can give out cold light or light absorbing optical signalling or luminous product L and generate with the added matter SUB that is subjected to.
First order biomolecule can be interosculated with second level biomolecule through object to be measured and be formed bioplex BIO-CO (shown in 6B figure), but other non-detection target non-TA (shown in 6A, 6B figure) then can not produce any reaction, therefore is suspended in the solution.Subsequently, flow passage structure M put that system's universal stage (not icon) is gone up and be that high speed rotating is carried out in the axle center along the filling orifice b0 of flow passage structure M, then after tested sample K (blood) is by the second channel V2 (ending to valve), then can be owing to the difference of composition centrifugal force, caused of the layering of second ingredient k 02 (blood cell) with first ingredient k 01 (blood plasma), second ingredient k 02 (blood cell) can be accumulated in the downside of the 3rd room C3 (eccentric groove), and first ingredient k 01 (blood plasma) then can be stored in the upside of the 3rd room C3 (eccentric groove).
Owing to surpassed the aperture of the second channel V2 (ending) with the volume that detects a plurality of object Q (glass microballoon) that target combined to valve, a plurality of object Q (glass microballoon) be blocked and rest on the 3rd room C3 (eccentric groove) in.Other not with it the luminous stain of combination then can be accompanied by the downside that first ingredient k 01 (blood plasma) together flows into the 3rd room C3 (eccentric groove), and the capillary strength by fluid channel, first ingredient k 01 (blood plasma) can be passed through turnover channel V3 in regular turn, and flows among the 4th room C4 (capillary channel) the 5th room C5 (detecting groove) that the rear end connected.
See also Fig. 6 A, 6B, 6C.Fig. 6 A, 6B, 6C be shown among the analytic system Z of the present invention the synoptic diagram of the biochemical reaction of being correlated with and optical detection.
As shown in Figure 6A, existing a plurality of object Q (glass microballoon) that add and demarcation molecule MOL in the second room C2 (dividing equally groove), and the location means optical detection system above or below the second room C2 (dividing equally groove), the luminous signal in fechtable to the five room C5 (detecting groove).When the specific target molecules among the tested sample K occurs, shown in Fig. 6 B, because a plurality of object Q of surface-treated (glass microballoon) can engage smoothly, then having the second order reaction thing of demarcating molecule MOL also can be connected in regular turn, therefore go up at a plurality of object Q (glass microballoon) and form a bioplex, shown in Fig. 6 C, other not engaged biomolecule then can enter in the detection zone under the driving by centrifugal force with demarcation molecule MOL, the optical system that is positioned at the 5th room C5 (detecting groove) is differentiated quantity and the concentration of demarcation molecule MOL to be measured according to luminous intensity, and the luminous signal intensity of cooperation previously-known fixed total amount, so just can know the actual quantity real and target molecule that a plurality of object Q (glass microballoon) are reacted by inference.
Though the present invention discloses as above with all embodiment; right its is not in order to restriction the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; change and retouching when doing, thus protection scope of the present invention when with claim the person of being defined be as the criterion.
Claims (69)
1. flow passage structure is used for separating between having of a tested sample one first composition of different nature and one second composition, and this flow passage structure comprises:
One first room;
One second room is connected in this first room and can rotates with respect to an axis of reference, and when this tested sample was arranged at this first room, this tested sample transferred to this second room;
One the 3rd room, be connected in this second room, the 3rd room has comprised interconnective one first buffer zone and one second buffer zone, when this second room with respect to this axis of reference and when a first direction rotates, the position is delivered to the 3rd room at this tested sample of this second room at one first given time, and separating between the position produces this tested sample at one second given time at this tested sample of this first buffer zone of the 3rd room this first composition and this second composition, wherein, this second given time is later than this first given time, and this second buffer zone of the 3rd room has been full of this separated first composition; And
One the 4th room, be connected in the 3rd room, when this second room stop operating with respect to this axis of reference and the quiet special time that stagnates after, the position is delivered to the 4th room at this first composition of this tested sample of this second buffer zone of the 3rd room, and when this second room from this stationary state and and when a second direction that is different from this first direction is rotated with respect to this axis of reference, outwards transmit via the 4th room by an acting force at this separated first composition of the 4th room the position, so makes this first composition be located away from this second composition fully.
2. flow passage structure as claimed in claim 1, also comprised one the 5th room, the 5th room is connected in the 4th room, when this second room from this stationary state and with respect to this axis of reference when this second direction is rotated, the position transfers to the 5th room at this separated first composition of the 4th room by an acting force, so makes this first composition be located away from this second composition fully.
3. flow passage structure as claimed in claim 1 also comprises one first channel, and this first channel is connected between this first room and this second room.
4. flow passage structure as claimed in claim 1 also comprises one second channel, and this second channel is arranged between this second room and the 3rd room.
5. flow passage structure as claimed in claim 4, wherein, this second channel is with respect to this axis of reference and along radial distribution.
6. flow passage structure as claimed in claim 4, wherein, this second channel is a capillary channel.
7. flow passage structure as claimed in claim 1, wherein, when this second room stopped operating with respect to this axis of reference, the position automatically was delivered to the 4th room in this first components utilising capillary action of this tested sample of this second buffer zone of the 3rd room.
8. flow passage structure as claimed in claim 1, wherein, this second buffer zone of the 3rd room comprises a line style capillary channel.
9. flow passage structure as claimed in claim 1, wherein, the 4th room comprises a line style capillary channel.
10. flow passage structure as claimed in claim 1 wherein, has one first angle between this first buffer zone of the 3rd room and this second buffer zone.
11. flow passage structure as claimed in claim 10, wherein, this first angle is not more than 30 degree.
12. flow passage structure as claimed in claim 1 wherein, has one second angle between this second buffer zone of the 3rd room and the 4th room.
13. flow passage structure as claimed in claim 10, wherein, this second angle is not less than 90 degree.
14. flow passage structure as claimed in claim 1 also comprises a turnover channel, this turnover channel position is between this second buffer zone and the 4th room of the 3rd room.
15. flow passage structure as claimed in claim 1, wherein, this first buffer zone of this second room and the 3rd room is with respect to this axis of reference and along radial distribution.
16. flow passage structure as claimed in claim 1 also comprises one the 6th room, the 6th room is connected in the 3rd room and the 4th room.
17. flow passage structure as claimed in claim 1, also comprise a main body with a basal plane, wherein, this first buffer zone of this first room, this second room, the 3rd room and this second buffer zone and the 4th room are the multi-groove structure on this basal plane that is formed at this main body jointly.
18. flow passage structure as claimed in claim 17, wherein, this second buffer zone of the 3rd room and the groove constructional depth of the 4th room are less than the groove constructional depth of this first buffer zone of this first room and the 3rd room.
19. flow passage structure as claimed in claim 1, wherein, this first buffer zone of the 3rd room has comprised an interconnective first area and a second area, and this first area is connected in this second room, and has channel degree of depth offset between this first area and this second area.
20. flow passage structure as claimed in claim 19, also comprise a zone line, this zone line position is between this first area and this second area, and between this first area and this zone line, have channel degree of depth offset respectively between this second area and this zone line, this second buffer zone is connected in this zone line.
21. flow passage structure as claimed in claim 1, wherein, this acting force has comprised a Ke Shi power that is produced by a coriolis acceleration.
22. flow passage structure as claimed in claim 1, wherein, this tested sample is subjected to respect to the effect of an accelerated motion of this axis of reference at this first given time and moves.
23. flow passage structure as claimed in claim 1, wherein, this tested sample is subjected to respect to the effect of a constant speed of this axis of reference motion and moves at this second given time.
24. flow passage structure as claimed in claim 1, wherein, the proportion of this first composition is different from the proportion of this second composition.
25. an analytic system comprises:
One working fluid has comprised having one first composition of different nature and one second composition;
One equal subdivision can rotate with respect to an axis of reference, and this equal subdivision is divided equally for this working fluid; And
One separative element, in order to carry out centrifugal the separation between position this first composition in this working fluid of this equal subdivision and this second composition, this separative element comprises:
One eccentric groove, interconnective one first buffer zone and one second buffer zone have been comprised, when this equal subdivision with respect to this axis of reference and when a first direction rotates, the position is delivered to this eccentric groove at this working fluid of this equal subdivision at one first given time, and separating between the position produces this working fluid at one second given time at this first buffer zone of this eccentric groove and this working fluid of this second buffer zone this first composition and this second composition, wherein, this second given time is later than this first given time, and this second buffer zone of this eccentric groove has been full of this first composition of this working fluid; And
One separates channel, be connected in this eccentric groove, when this equal subdivision stop operating with respect to this axis of reference and the quiet special time that stagnates after, the position is delivered to this at this first buffer zone of this eccentric groove with this first composition of this working fluid of this second buffer zone and separates channel, and when this equal subdivision from this stationary state and and when a second direction that is different from this first direction is rotated with respect to this axis of reference, the position is separated channel centrifugal outside transmission by an acting force via this at this first composition that this separates this working fluid of channel, so makes this first composition be located away from this second composition fully.
26. analytic system as claimed in claim 25, wherein, this separative element has also comprised a detecting groove, this detecting groove is connected in this separation channel, when this equal subdivision from this stationary state and with respect to this axis of reference when this second direction is rotated, the position transfers to this detecting groove at this first composition of this working fluid of this separation channel by an acting force, so makes this first composition be located away from this second composition fully.
27. analytic system as claimed in claim 25, wherein, the proportion of this first composition is different from the proportion of this second composition.
28. analytic system as claimed in claim 25, wherein, this working fluid comprises a blood, and this first composition comprises blood plasma, and this second composition comprises blood cell.
29. analytic system as claimed in claim 25, wherein, this separative element also comprises one second channel, and this second channel is arranged between this equal subdivision and this eccentric groove.
30. analytic system as claimed in claim 29, wherein, this second channel is with respect to this axis of reference and along radial distribution.
31. analytic system as claimed in claim 29, wherein, this second channel is a capillary channel.
32. analytic system as claimed in claim 25, wherein, when this equal subdivision stopped operating with respect to this axis of reference, the position automatically was delivered to this separation channel in this first components utilising capillary action of this working fluid of this second buffer zone of this eccentric groove.
33. analytic system as claimed in claim 25, wherein, this of this eccentric groove second buffer zone comprises a line style capillary channel.
34. analytic system as claimed in claim 25, wherein, this separation channel comprises a line style capillary channel.
35. analytic system as claimed in claim 25 wherein, has one first angle between this of this eccentric groove first buffer zone and this second buffer zone.
36. analytic system as claimed in claim 35, wherein, this first angle is not more than 30 degree.
37. analytic system as claimed in claim 25, wherein, this of this eccentric groove second buffer zone separates with this and has one second angle between channel.
38. analytic system as claimed in claim 37, wherein, this second angle is not less than 90 degree.
39. analytic system as claimed in claim 25, wherein, this separative element also comprises a turnover channel, and separate between the channel with this at this second buffer zone of this eccentric groove this turnover channel position.
40. analytic system as claimed in claim 25, wherein, this first buffer zone of this equal subdivision and this eccentric groove is with respect to this axis of reference and along radial distribution.
41. analytic system as claimed in claim 25, wherein, this separative element also comprises an air discharge duct, and this air discharge duct is connected in this eccentric groove and separates channel with this.
42. analytic system as claimed in claim 25, also comprise a main body with a basal plane, wherein, this equal subdivision and this first buffer zone of this eccentric groove of separative element separate channel and are the multi-groove structure on this basal plane that is formed at this main body jointly with this second buffer zone, this.
43. analytic system as claimed in claim 25, wherein, this of this eccentric groove second buffer zone separates the groove constructional depth of the groove constructional depth of channel much smaller than this first buffer zone of this eccentric groove with this.
44. analytic system as claimed in claim 25, wherein, this of this eccentric groove first buffer zone has comprised an interconnective first area and a second area, and this first area is connected in this equal subdivision, and has channel degree of depth offset between this first area and this second area.
45. analytic system as claimed in claim 44, also comprise a zone line, this zone line position is between this first area and this second area, and between this first area and this zone line, have channel degree of depth offset respectively between this second area and this zone line, this second buffer zone is connected in this zone line.
46. analytic system as claimed in claim 25, wherein, this acting force has comprised a Ke Shi power that is produced by a coriolis acceleration.
47. analytic system as claimed in claim 25, wherein, this working fluid is subjected to respect to the effect of an accelerated motion of this axis of reference at this first given time and moves.
48. analytic system as claimed in claim 25, wherein, this working fluid is subjected to respect to the effect of a constant speed of this axis of reference motion and moves at this second given time.
49. analytic system as claimed in claim 25, a plurality of objects have also been comprised with one first demarcation thing, these objects be arranged at this equal subdivision in, and this tested sample comprises that also one second demarcates thing, this of this tested sample second demarcate between this first demarcation things of thing and these objects can in conjunction with.
50. analytic system as claimed in claim 49, wherein, these objects comprise carriers such as glass spheres, magnetic bead.
51. analytic system as claimed in claim 49, wherein, this first demarcation thing has comprised nucleic acid, protein, biomarker molecule, antibody, cell or other biomolecule with joint.
52. analytic system as claimed in claim 49, wherein, this second is demarcated nucleic acid that thing comprised the complementation of tool mark function, is subjected to matter, ferment, coenzyme, complement, antigen, other cell or biomolecule.
53. an analytical approach may further comprise the steps:
Provide and comprised a working fluid with one first composition of different nature and one second composition;
Provide an equal subdivision to rotate with respect to an axis of reference, this equal subdivision is divided equally for this working fluid; And
Provide an eccentric groove to separate channel with one, this eccentric groove separates channel and carries out centrifugal the separation between position this first composition in this working fluid of this equal subdivision and this second composition in order with this, wherein, this eccentric groove has comprised interconnective one first buffer zone and one second buffer zone, when this equal subdivision with respect to this axis of reference and when a first direction rotates, the position is delivered to this eccentric groove at this working fluid of this equal subdivision at one first given time, and separating between the position produces this working fluid at one second given time at this first buffer zone of this eccentric groove and this working fluid of this second buffer zone this first composition and this second composition, wherein, this second given time is later than this first given time, and this second buffer zone of this eccentric groove has been full of this first composition of this working fluid; This separation channel is connected in this eccentric groove, when this equal subdivision stop operating with respect to this axis of reference and the quiet given time that stagnates after, the position is delivered to this at this first buffer zone of this eccentric groove with this first composition of this working fluid of this second buffer zone and separates channel, and when this equal subdivision from this stationary state and and when a second direction that is different from this first direction is rotated with respect to this axis of reference, the position is separated channel centrifugal outside transmission by an acting force via this at this first composition that this separates this working fluid of channel, so makes this first composition be located away from this second composition fully.
54. analytical approach as claimed in claim 53, one detecting groove more is provided, this detecting groove is connected in this separation channel, when this equal subdivision from this stationary state and and when this second direction is rotated with respect to this axis of reference, the position transfers to this detecting groove at this first composition of this working fluid of this separation channel by an acting force, so makes this first composition be located away from this second composition fully.
55. analytical approach as claimed in claim 53 more provides one second channel, this second channel is arranged between this equal subdivision and this eccentric groove.
56. analytical approach as claimed in claim 53, wherein, this second channel is with respect to this axis of reference and along radial distribution.
57. analytical approach as claimed in claim 53, wherein, when this equal subdivision stopped operating with respect to this axis of reference, the position automatically was delivered to this separation channel in this first components utilising capillary action of this working fluid of this second buffer zone of this eccentric groove.
58. analytical approach as claimed in claim 53 wherein, provides one first angle between this of this eccentric groove first buffer zone and this second buffer zone.
59. analytical approach as claimed in claim 58, wherein, this first angle is not more than 30 degree.
60. analytical approach as claimed in claim 53, wherein, this of this eccentric groove second buffer zone separates with this provides one second angle between channel.
61. analytical approach as claimed in claim 60, wherein, this second angle is not less than 90 degree.
62. analytical approach as claimed in claim 53 more provides a turnover channel, separate between the channel with this at this second buffer zone of this eccentric groove this turnover channel position.
63. analytical approach as claimed in claim 53, wherein, this acting force has comprised a Ke Shi power that is produced by a coriolis acceleration.
64. analytical approach as claimed in claim 53, wherein, this working fluid is subjected to respect to the effect of an accelerated motion of this axis of reference at this first given time and moves.
65. analytical approach as claimed in claim 53, wherein, this working fluid is subjected to respect to the effect of a constant speed of this axis of reference motion and moves at this second given time.
66. analytical approach as claimed in claim 53, a plurality of objects with one first demarcation thing more are provided, these objects be arranged at this equal subdivision in, and this tested sample comprises that also one second demarcates thing, this of this tested sample second demarcate between this first demarcation things of thing and these objects can in conjunction with.
67. as the described analytical approach of claim 66, wherein, this first demarcation thing has comprised nucleic acid, protein, biomarker molecule, antibody, cell or other biomolecule with joint.
68. as the described analytical approach of claim 66, wherein, this second is demarcated nucleic acid that thing comprised the complementation of tool mark function, is subjected to matter, ferment, coenzyme, complement, antigen, other cell or biomolecule.
69. analytical approach as claimed in claim 53, wherein, the proportion of this first composition is different from the proportion of this second composition.
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| WO2013053077A1 (en) * | 2011-10-10 | 2013-04-18 | 保生国际生医股份有限公司 | Centrifugal rotating disk |
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