CN101597585B - Culture medium suitable for industrial production of bifidobacteria and preparation method thereof - Google Patents
Culture medium suitable for industrial production of bifidobacteria and preparation method thereof Download PDFInfo
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- CN101597585B CN101597585B CN 200910136516 CN200910136516A CN101597585B CN 101597585 B CN101597585 B CN 101597585B CN 200910136516 CN200910136516 CN 200910136516 CN 200910136516 A CN200910136516 A CN 200910136516A CN 101597585 B CN101597585 B CN 101597585B
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- steep liquor
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- bifidobacteria
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- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 17
- 239000001963 growth medium Substances 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000009776 industrial production Methods 0.000 title claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 42
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 42
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 41
- 235000005822 corn Nutrition 0.000 claims abstract description 41
- 239000000843 powder Substances 0.000 claims abstract description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 239000008400 supply water Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims 3
- 239000012467 final product Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 abstract description 2
- 229920000053 polysorbate 80 Polymers 0.000 abstract description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 19
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- 238000012216 screening Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 108010046377 Whey Proteins Proteins 0.000 description 3
- 102000007544 Whey Proteins Human genes 0.000 description 3
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- 235000018102 proteins Nutrition 0.000 description 3
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- 235000020183 skimmed milk Nutrition 0.000 description 3
- 235000021119 whey protein Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 235000021336 beef liver Nutrition 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
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- 235000019319 peptone Nutrition 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
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- 239000011573 trace mineral Substances 0.000 description 2
- 108010046845 tryptones Proteins 0.000 description 2
- QIJRTFXNRTXDIP-UHFFFAOYSA-N (1-carboxy-2-sulfanylethyl)azanium;chloride;hydrate Chemical compound O.Cl.SCC(N)C(O)=O QIJRTFXNRTXDIP-UHFFFAOYSA-N 0.000 description 1
- 108010053481 Antifreeze Proteins Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
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- 239000006173 Good's buffer Substances 0.000 description 1
- 240000008892 Helianthus tuberosus Species 0.000 description 1
- 235000003230 Helianthus tuberosus Nutrition 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
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- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 239000008120 corn starch Substances 0.000 description 1
- 229960001305 cysteine hydrochloride Drugs 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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- 238000004108 freeze drying Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 230000020245 homoiothermy Effects 0.000 description 1
- 230000037451 immune surveillance Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 210000002784 stomach Anatomy 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
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Priority Applications (1)
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CN 200910136516 CN101597585B (en) | 2009-05-06 | 2009-05-06 | Culture medium suitable for industrial production of bifidobacteria and preparation method thereof |
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CN 200910136516 CN101597585B (en) | 2009-05-06 | 2009-05-06 | Culture medium suitable for industrial production of bifidobacteria and preparation method thereof |
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CN101597585A CN101597585A (en) | 2009-12-09 |
CN101597585B true CN101597585B (en) | 2013-04-10 |
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CN 200910136516 Active CN101597585B (en) | 2009-05-06 | 2009-05-06 | Culture medium suitable for industrial production of bifidobacteria and preparation method thereof |
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Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107760635A (en) * | 2017-11-30 | 2018-03-06 | 成都欧林生物科技股份有限公司 | Beneficial to the bacterium culture medium of anti-microbiological contamination |
CN112592860A (en) * | 2020-12-23 | 2021-04-02 | 深圳科兴药业有限公司 | Bifidobacterium infantis fermentation medium without animal-derived nitrogen source and application thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1400304A (en) * | 2002-07-16 | 2003-03-05 | 李盛学 | Liquid-solid combined fermentation process of probiotics by using bifidobacteria as main component |
-
2009
- 2009-05-06 CN CN 200910136516 patent/CN101597585B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1400304A (en) * | 2002-07-16 | 2003-03-05 | 李盛学 | Liquid-solid combined fermentation process of probiotics by using bifidobacteria as main component |
Non-Patent Citations (4)
Title |
---|
双歧杆菌BBMN02培养基优化及发酵罐补料分批培养;田寒友等;《中国乳业》;20081025(第10期);50-53 * |
孙纪录等.菊芋汁双歧杆菌培养基的筛选优化.《食品科学》.2003,第24卷(第11期),76-79. |
田寒友等.双歧杆菌BBMN02培养基优化及发酵罐补料分批培养.《中国乳业》.2008,(第10期),50-53. |
菊芋汁双歧杆菌培养基的筛选优化;孙纪录等;《食品科学》;20031130;第24卷(第11期);76-79 * |
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CN101597585A (en) | 2009-12-09 |
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Inventor after: Zhang Hao Inventor after: Tian Hanyou Inventor after: Ren Fazheng Inventor after: Jiang Jingli Inventor after: Liu Aiping Inventor after: Yang Haiying Inventor after: Zhao Liang Inventor after: Zhang Dingding Inventor after: Liu Songling Inventor before: Tian Hanyou Inventor before: Ren Fazheng Inventor before: Jiang Jingli Inventor before: Liu Aiping Inventor before: Yang Haiying Inventor before: Zhao Liang Inventor before: Zhang Dingding Inventor before: Liu Songling |
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Free format text: CORRECT: INVENTOR; FROM: TIAN HANYOU REN FAZHENG JIANG JINGLI LIU AIPING YANG HAIYING ZHAO LIANG ZHANG DINGDING LIU SONGLING TO: ZHANG HAO TIAN HANYOU REN FAZHENG JIANG JINGLI LIU AIPING YANG HAIYING ZHAO LIANG ZHANG DINGDING LIU SONGLING |
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Inventor after: Ren Fazheng Inventor after: Tian Hanyou Inventor after: Jiang Jingli Inventor after: Liu Aiping Inventor after: Yang Haiying Inventor after: Zhao Liang Inventor after: Zhang Dingding Inventor after: Liu Songling Inventor before: Zhang Hao Inventor before: Tian Hanyou Inventor before: Ren Fazheng Inventor before: Jiang Jingli Inventor before: Liu Aiping Inventor before: Yang Haiying Inventor before: Zhao Liang Inventor before: Zhang Dingding Inventor before: Liu Songling |
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Free format text: CORRECT: INVENTOR; FROM: ZHANG HAO TIAN HANYOU REN FAZHENG JIANG JINGLI LIU AIPING YANG HAIYING ZHAO LIANG ZHANG DINGDING LIU SONGLING TO: REN FAZHENG TIAN HANYOU JIANG JINGLI LIU AIPING YANG HAIYING ZHAO LIANG ZHANG DINGDING LIU SONGLING |
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Effective date of registration: 20150227 Address after: 100083 Haidian District Qinghua East Road, No. 17, Beijing Patentee after: China Agricultural University Address before: 100083 School of food science and Nutritional Engineering, China Agricultural University, 17 Qinghua Dong Road, Beijing, Haidian District Patentee before: Ren Fazheng |
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Effective date of registration: 20171113 Address after: 344000 Jiangxi city in Fuzhou Province Economic Development Zone Wenchang Jinchao Avenue East five road south latitude Patentee after: JIANGXI SHUMEITE PHARMACEUTICAL Co.,Ltd. Address before: 100083 Haidian District Qinghua East Road, No. 17, Beijing Patentee before: China Agricultural University |
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Denomination of invention: Culture medium suitable for industrial production of bifidobacteria and preparation method thereof Effective date of registration: 20190409 Granted publication date: 20130410 Pledgee: Ganzhou Bank Co.,Ltd. Fuzhou Branch Pledgor: JIANGXI SHUMEITE PHARMACEUTICAL Co.,Ltd. Registration number: 2019360000016 |
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Date of cancellation: 20220706 Granted publication date: 20130410 Pledgee: Ganzhou Bank Co.,Ltd. Fuzhou Branch Pledgor: JIANGXI SHUMEITE PHARMACEUTICAL Co.,Ltd. Registration number: 2019360000016 |
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