CN101591695A - The sodium lauryl sulphate neutralization increases bacterial context soup - Google Patents
The sodium lauryl sulphate neutralization increases bacterial context soup Download PDFInfo
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- CN101591695A CN101591695A CNA2008101001519A CN200810100151A CN101591695A CN 101591695 A CN101591695 A CN 101591695A CN A2008101001519 A CNA2008101001519 A CN A2008101001519A CN 200810100151 A CN200810100151 A CN 200810100151A CN 101591695 A CN101591695 A CN 101591695A
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- lauryl sulphate
- sodium lauryl
- neutralization
- bacterial context
- context soup
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Abstract
The present invention relates to a kind of sodium lauryl sulphate neutralization and increase bacterial context soup, particularly a kind of sodium lauryl sulphate neutralization increases prescription of bacterial context soup and preparation method thereof.Its main component is that Tryptones, lactose, dipotassium hydrogen phosphate, potassium primary phosphate, saligenin, soil temperature-80, Triton X-100, glycine, sodium-chlor, sodium lauryl sulphate, distilled water are prepared from; Be used for and water and food, environmental sample in the intestinal bacteria check.
Description
Technical field
The present invention relates to a kind of sodium lauryl sulphate neutralization and increase bacterial context soup, particularly a kind of sodium lauryl sulphate neutralization increases prescription of bacterial context soup and preparation method thereof.Its main component is that Tryptones, lactose, dipotassium hydrogen phosphate, potassium primary phosphate, saligenin, soil temperature-80, Triton X-100, glycine, sodium-chlor, sodium lauryl sulphate, distilled water are prepared from; Be used for and water and food in the intestinal bacteria check.The experiment that increases the bacterium cultivation of any one environmental sample all must have the reliable remaining sterilizing agent and the method for antimicrobial factors removed.Do not adopt and remove the remaining sterilizing agent and the experiment of antimicrobial factors measure, should think serious mistake in design, the result who draws according to such experiment is insecure.The chemistry neutralisation is sterilizing agent and the antimicrobial factors method of at present the most frequently used removal remnants, and its advantage is that method is simple, and is easy to use, reliable for effect.Existing neutralization method is a lot, and common have acid, alkali, absorption, dilution, centrifugal a, filtration etc.The above-mentioned screening of all passing through strictness also can't not determined its working concentration; Corresponding sterilizing agent and antimicrobial factors do not had solid neutralizing effect; Neutralizing agent itself or with the product of sterilizing agent and antimicrobial factors reaction the sample microorganism is had killing or suppress the effect of its growth; To cultivating composition destruction is arranged, also influence its physical behavior.Substratum is the suitable microorganism growth breeding of artificial preparation or the nutraceutical matrix of accumulation meta-bolites, with regard to nutritive substance, generally nothing more than several big classes such as carbon source, nitrogenous source, inorganic salt, somatomedin and water.In preparation during substratum,, just can make the suitable different types of microorganisms needed substratum that grows according to the characteristics of each quasi-microorganism.Substratum also requires to have certain potential of hydrogen and osmotic pressure except satisfying the essential nutritive substance of Institute of Micro-biology.Each quasi-microorganism is not quite similar to the requirement of nutrition, thereby substratum is of a great variety.The preparation procedure method of substratum, can be different different because of its kind, even substratum with a kind of prescription, each laboratory and manufacturer also can adjust to some extent according to separately the experience and the difference of raw materials quality, therefore, before preparation, should carry out a small amount of allotment, prerun earlier, meet service requirements, implement preparation by the prescription materials of determining again.Test work person wishes to have always and a kind ofly can overcome above-mentioned insufficient neutralization and increase bacterial context soup, and this substratum is nutritious, can stimulate its growth and aerogenesis to inoculating a small amount of coliform.Aerobic spore-bearing bacilli then is suppressed entirely, can be directly used in indole test, when doing indole test, after should adding a small amount of ether in advance and jolting, add one deck indoles reagent, do not jolting, looking two liquid contact surfaces has or not red-purple to occur, if do not add diethyl ether, then easily form persistent emulsification and disturb indole test, this is because of there being the relation of sodium lauryl sulphate.Also being used for increasing bacterium with sample cultivates.
Background technology
The object of the present invention is to provide a kind of sodium lauryl sulphate neutralization to increase bacterial context soup, this substratum is nutritious, the mensuration of suitable entero-bacte; Be used for sample and increase the bacterium cultivation, increase bacterium with sample in can be used for and cultivate.Sodium lauryl sulphate neutralization increases that bacterial context soup is nutritious, preparation is simple, selectivity is strong, with traditional substratum relatively, the recall rate height pollutes for a short time, clinical application effect is satisfied. improve the detection positive rate greatly, method is simple, the time is short, accuracy is high.
Summary of the invention
Main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technologies such as mixing, cooling, packing, high-temperature sterilization form a kind of sodium lauryl sulphate neutralization and increase bacterial context soup.
The culture medium prescription that the present invention selects following (gram, every liter of distilled water of ml/) Tryptones 20.0-22.0; Lactose 5.0-5.5; Dipotassium hydrogen phosphate 2.70-2.75; Potassium primary phosphate 2.70-2.75; Saligenin 0.5-0.6; Soil temperature-80 1.0-1.2; TritonX-1001.0-1.2; Glycine 0.5-0.6; Sodium-chlor 5.0-5.5; Sodium lauryl sulphate 0.1-0.2; Distilled water is to 1000mL.
Tryptones can stimulate its growth and aerogenesis to inoculating a small amount of coliform.Aerobic spore-bearing bacilli then is suppressed entirely, can be directly used in indole test, when doing indole test, after should adding a small amount of ether in advance and jolting, add one deck indoles reagent, do not jolting, looking two liquid contact surfaces has or not red-purple to occur, if do not add diethyl ether, then easily form persistent emulsification and disturb indole test, this is because of there being the relation of sodium lauryl sulphate.
The sodium lauryl sulphate neutralization increases bacterial context soup, starting material are easy to get, easy to make, basic ingredient adds nutritious beef extract powder, yeast extract powder, the yeast extract powder is mainly the microorganism growth breeding solubility nitrogenous substances, nucleic acid degradation product, VITAMIN and inorganic salts etc. is provided, to replenish the insufficient section of peptone nutritive ingredient.
The bacteriostatic action of sulfa drugs and sterilizing agent in saligenin, soil temperature-80, Triton X-100, the glycine and in the sample; Sodium lauryl sulphate is destroyed the bacteriostatic action of the tsiklomitsin, duomycin, Xin Meisu, polymyxin and the Streptomycin sulphate that exist in the sample.
This substratum of Quality Control is light amber, and is transparent, and flocks can be arranged.After the Quality Control inoculation, two kinds of temperature are cultivated 18 ~ 24h, the following aerogenesis intestinal bacteria of result (ATCC13048) well-grown, escherichia coli (ATCC25922) well-grown, Salmonella typhimurtum (ATCC14028) well-grown, streptococcus aureus (ATCC25923) well-grown.
The preparation method of product of the present invention is:
1), each composition is mixed in the water, heating for dissolving is transferred pH6.6 ~ 7.0,
2), be sub-packed in be placed with the fermentation voltage regulator tube test tube, every pipe 10mL, 121 ℃ of 15min autoclavings are standby.
3), the Quality Control bacterial strain is aerogenesis intestinal bacteria (ATCC13048), escherichia coli (ATCC25922), Salmonella typhimurtum (ATCC14028), streptococcus aureus (ATCC25923) and the parallel sensitivity test of carrying out bacterial growth of corresponding qualified control medium, should meet the requirements.
The present invention has the following advantages: (1) sodium lauryl sulphate neutralization increases bacterial context soup, and is nutritious, preparation is simple; (2) selectivity is strong, method is sensitive, recall rate is high, pollution is little, and clinical application effect is satisfied; (3) improve the detection positive rate greatly, method is simple, the time is short, accuracy is high.(4) highly sensitive, high specificity, good stability.
Embodiment
The present invention is described in further detail below in conjunction with embodiment:
Be configured according to following table institute column data (gram, every liter of distilled water of ml/) and described step thereof:
Prescription one:
Tryptones 20.0-22.0
Lactose 5.0-5.5
Dipotassium hydrogen phosphate 2.70-2.75
Potassium primary phosphate 2.70-2.75
Saligenin 0.5-0.6
Soil temperature-80 1.0-1.2
Triton?X-100 1.0-1.2
Glycine 0.5-0.6
Sodium-chlor 5.0-5.5
Sodium lauryl sulphate 0.1-0.2
Distilled water 1000mL
Prescription two:
Tryptones 20.0-22.0
Lactose 5.2-5.5
Dipotassium hydrogen phosphate 2.72-2.75
Potassium primary phosphate 2.71-2.75
Saligenin 0.55-0.6
Soil temperature-80 1.1-1.2
Triton?X-100 1.1-1.2
Glycine 0.55-0.6
Sodium-chlor 5.2-5.5
Sodium lauryl sulphate 0.15-0.2
Distilled water 1000mL
Prescription three:
Tryptones 20.0-21.0
Lactose 5.0-5.4
Dipotassium hydrogen phosphate 2.70-2.74
Potassium primary phosphate 2.70-2.73
Saligenin 0.5-0.57
Soil temperature-80 1.0-1.1
Triton?X-100 1.0-1.1
Glycine 0.5-0.58
Sodium-chlor 5.0-5.4
Sodium lauryl sulphate 0.1-0.12
Distilled water 1000mL
Prescription four:
Tryptones 21.0-22.0
Lactose 5.0-5.3
Dipotassium hydrogen phosphate 2.73-2.75
Potassium primary phosphate 2.70-2.74
Saligenin 0.55-0.6
Soil temperature-80 1.0-1.1
Triton?X-100 1.1-1.2
Glycine 0.5-0.55
Sodium-chlor 5.3-5.5
Sodium lauryl sulphate 0.16-0.2
Distilled water 1000mL
Claims (2)
1, the present invention relates to a kind of sodium lauryl sulphate neutralization and increase bacterial context soup, it is characterized in that having following prescription (gram, every liter of distilled water of ml/): Tryptones 20.0-22.0; Lactose 5.0-5.5; Dipotassium hydrogen phosphate 2.70-2.75; Potassium primary phosphate 2.70-2.75; Saligenin 0.5-0.6; Soil temperature-80 1.0-1.2; Triton X-1001.0-1.2; Glycine 0.5-0.6; Sodium-chlor 5.0-5.5; Sodium lauryl sulphate 0.1-0.2; Distilled water is to 1000mL.
2, the described sodium lauryl sulphate neutralization of a kind of claim 1 increases the preparation method of bacterial context soup, it is characterized in that having following step:
1), each composition is mixed in the water heating for dissolving, accent pH6.6-7.0;
2), be sub-packed in be placed with the fermentation voltage regulator tube test tube, every pipe 10mL, 121 ℃ of 15min autoclavings are standby;
3), the Quality Control bacterial strain is aerogenesis intestinal bacteria (ATCC13048), escherichia coli (ATCC25922), Salmonella typhimurtum (ATCC14028), streptococcus aureus (ATCC25923) and the parallel sensitivity test of carrying out bacterial growth of corresponding qualified control medium, should meet the requirements.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101001519A CN101591695A (en) | 2008-05-26 | 2008-05-26 | The sodium lauryl sulphate neutralization increases bacterial context soup |
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| CNA2008101001519A CN101591695A (en) | 2008-05-26 | 2008-05-26 | The sodium lauryl sulphate neutralization increases bacterial context soup |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101864383A (en) * | 2010-05-11 | 2010-10-20 | 佛山市海天调味食品有限公司 | Coliform bacterium and enzyme increasing culture solution and preparation method thereof |
| WO2012018341A1 (en) * | 2010-08-05 | 2012-02-09 | Family Health International | Device and method for delivering an agent into breast milk while breastfeeding |
| US8357117B2 (en) | 2008-08-06 | 2013-01-22 | Family Health International | Device and method for delivering an agent into breast milk while breastfeeding |
| CN104313114A (en) * | 2014-10-28 | 2015-01-28 | 吴学斌 | Detection kit and detection method for escherichia coli |
-
2008
- 2008-05-26 CN CNA2008101001519A patent/CN101591695A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8357117B2 (en) | 2008-08-06 | 2013-01-22 | Family Health International | Device and method for delivering an agent into breast milk while breastfeeding |
| CN101864383A (en) * | 2010-05-11 | 2010-10-20 | 佛山市海天调味食品有限公司 | Coliform bacterium and enzyme increasing culture solution and preparation method thereof |
| CN101864383B (en) * | 2010-05-11 | 2012-06-06 | 佛山市海天调味食品股份有限公司 | Coliform bacterium and enzyme increasing culture solution and preparation method thereof |
| WO2012018341A1 (en) * | 2010-08-05 | 2012-02-09 | Family Health International | Device and method for delivering an agent into breast milk while breastfeeding |
| CN104313114A (en) * | 2014-10-28 | 2015-01-28 | 吴学斌 | Detection kit and detection method for escherichia coli |
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Open date: 20091202 |