CN101584655A - High-purity salidroside injection preparation and preparation method thereof - Google Patents
High-purity salidroside injection preparation and preparation method thereof Download PDFInfo
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- CN101584655A CN101584655A CNA2008100444365A CN200810044436A CN101584655A CN 101584655 A CN101584655 A CN 101584655A CN A2008100444365 A CNA2008100444365 A CN A2008100444365A CN 200810044436 A CN200810044436 A CN 200810044436A CN 101584655 A CN101584655 A CN 101584655A
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Abstract
The invention provides high-purity salidroside injection preparation and preparation technology thereof, ejection preparation of the present invention comprises injection, lyophilized injectable powder and high capacity ejection preparation.High-purity salidroside injection preparation provided by the present invention has good effect in the treatment of cardiovascular and cerebrovascular disease, instant effect, bioavailability height, characteristics such as stable in properties.
Description
Technical field:
The present invention relates to salidroside injection preparation and preparation method thereof, the said ejection preparation of the present invention comprises liquid drugs injection, lyophilized injectable powder and bulk capacity injection, belongs to technical field of medicaments.
Technical background:
Radix Rhodiolae is the root and rhizome of Crassulaceae rhodiola (Rhodiola L.) plant, the Rhodida plant whole world again has 100 kinds approximately, can and abominable and changeable natural environment in grow, for a kind of adaptation former state plant, mainly be distributed in Himalaya mountain area, the northwestward, Asia and Bei Meizhou.China has 80 kinds approximately, is distributed in southwest, northwest, North, Northeast China area more.Discover at present and contain numerous effective ingredient such as rhodioside, pyrite, pyrosulfuric acid, gallic acid or the like in the Radix Rhodiolae, have anti-hypoxia, resisting fatigue, defying age, radioprotective, antitumor, blood fat reducing and prevent biological actions such as cardiovascular disease.In view of Radix Rhodiolae is having so many function and significant curative effect aspect preventing and curing diseases, make domestic and international many scholars one after another at the Radix Rhodiolae single component carry out structure and drug effect is carried out extensive and deep research.Rhodioside is the maximum effective ingredient of research in the Radix Rhodiolae, is one of main component of treatment cardiovascular and cerebrovascular disease, capable of inhibiting cell in calcium ion concentration raise, intervene neuronal apoptosis, neuroprotective cell and treat ischemic brain injury; Radical damage to the rat layer neurocyte has protective effect, can be used for ischemic cerebrovascular; Can obviously increase ischemic animal cardiac muscle content of glycogen, reduce the load effect of heart front and back, help improving and recovering cardiac function; It is stressed to improve myocardial ischemia oxygen, and myocardial cell injury is had protective effect etc.And toxicological experiment shows, rhodioside toxicity is very little: the mouse subcutaneous injection Radix Rhodiolae is not caused toxic reaction with 1g/kg; Mouse mainline rhodioside 3g/kg did not see any toxic reaction in continuous five days.
Cardiovascular and cerebrovascular disease is commonly encountered diseases, frequently-occurring disease clinically.In recent years, raising along with people's living standard, add the aging of population structure, its sickness rate is the trend that rises year by year, the toxicity of exploitation, the medicine of the cardiovascular and cerebrovascular disease of high therapeutic effect has become the focus of current drug research, and increasing clinical research report shows: high-purity single component rhodioside has a good application prospect at the treatment treating cardiac and cerebral vascular diseases.But also there is not a kind of ejection preparation application in this respect of highly purified rhodioside at present.
Summary of the invention:
First purpose of the present invention is to provide a kind of highly purified rhodioside and preparation technology thereof; Second purpose of the present invention is to provide high-purity salidroside aqueous injection, lyophilized injectable powder and high capacity ejection preparation and preparation technology thereof; Ejection preparation of the present invention comprises.Salidroside injection preparation provided by the present invention has good effect, instant effect, bioavailability height, characteristics such as stable in properties.
The present invention constitutes like this:
1. highly purified rhodioside preparation technology of the present invention is:
------------------the alcohol dissolving---is filtered---crystallization---high-purity salidroside to chromatography to adsorbent resin to eluting, concentrated to adsorbent resin to the Radix Rhodiolae crude extract to concentrate evaporate to dryness
Concrete preparation technology is:
(1) Radix Rhodiolae crude extract temperature is 20～90 ℃ a water stirring and dissolving 20 minutes～3 hours, filter and obtain clear filtrate,
(2) regulate filtrate pH value=1～5, adsorb with adsorbent resin under the room temperature, use 10%～40% acetone, low-carbon alcohols or acetate esters organic solvent eluting then
(3) concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously.
(4) adsorbent resin after will drying joins in the chromatographic column of having filled adsorbent resin, and room temperature carries out chromatography with 5%～25% low pure carbon class organic solvent down, and collection chromatography permeate is with its concentrated evaporate to dryness.
(5) add low-carbon alcohols or acetate esters organic solvent heating for dissolving, heating-up temperature is no more than 60 ℃, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, dry below 70 ℃, obtain highly purified rhodioside goods, the purity of these rhodioside goods reaches more than 90%.
2. highly purified rhodioside hydro-acupuncture preparation of the present invention, component percentages is calculated by weight, and it is by high-purity salidroside 0.2%～10%, sodium chloride 0.5%～1.5%, chlorobutanol 0.2%～0.5%, surplus is a water for injection.
Preferably: it is by high-purity salidroside 0.5%～1.5%, sodium chloride 0.7%～1.2%, and chlorobutanol 0.2%～0.4%, surplus is a water for injection.
Its preparation technology is:
Preparation method of the present invention is: in dispensing containers, adds proper amount of water for injection, adds rhodioside successively, and sodium chloride, chlorobutanol, stirring and dissolving adds the injection water, filters, and potting is in ampoule, and sterilization is promptly.
Concrete preparation technology is: in dispensing containers, add the water for injection of configuration amount 80%, add rhodioside, sodium chloride successively, chlorobutanol, stirring are to dissolve fully, add the injection water to scale, filtering with microporous membrane, potting are in the ampoule of 2ml, and 100 ℃ of sterilization 30min promptly.
3. highly purified rhodioside injectable powder of the present invention, component percentages is calculated by weight, and it is by high-purity salidroside 0.2%～10%, mannitol or dextran or lactose 2%～10%, water for injection 78.0%～97.0%.
Be preferably: high-purity salidroside 1%～8%, mannitol or dextran or lactose 4%～8%, water for injection consumption are 90.0%～95.8%.
Preparation technology of the present invention is: take by weighing rhodioside, and mannitol or dextran or lactose, and place sterile chamber, and add an amount of water for injection dissolving, add active carbon, filter; The filtrate heating in water bath adds the water for injection of capacity again, filters, and regulates PH, packing, and vacuum lyophilization promptly obtains Hongjingtian glycoside freeze dried powder injection agent.
Concrete preparation technology is: take by weighing rhodioside, and mannitol or dextran or lactose, and place sterile chamber, add an amount of water for injection dissolving, make material solution, percent by volume is 0.1%～0.5% to add active carbon in material solution by weight, filters; The filtrate water-bath is heated to 55 ℃～65 ℃, at 10 ℃～60 ℃, places 20～60 minutes, adds water for injection again to proportion of composing, regulates PH about about 6, uses filtering with microporous membrane, is sub-packed in the cillin bottle false add butyl rubber plug; Place freeze drying box to be cooled to-40 ℃～-45 ℃ cillin bottle, kept 4～6 hours, be evacuated to-300～600mmHg, the dividing plate heating, make temperature rise to-15 ℃～-19 ℃, kept 4～6 hours, be warming up to 20～30 ℃, be incubated 5～7 hours, vacuum is promptly made Hongjingtian glycoside freeze dried powder injection agent after rolling lid.
4. highly purified rhodioside bulk capacity injection of the present invention, component percentages is calculated by weight, and it is by high-purity salidroside 0.2%～10%, sodium chloride 0.5%～1.5%, surplus is a water for injection.
Preferably: it is by high-purity salidroside 0.5%～1.5%, sodium chloride 0.7%～1.2%, and surplus is a water for injection.
Preparation method of the present invention is: in dispensing containers, adds proper amount of water for injection, adds rhodioside successively, and sodium chloride, stirring is to dissolve fully, adds the injection water, filter, potting, sterilization is promptly.
Concrete preparation technology is: in dispensing containers, adds the water for injection of configuration amount 80%, adds rhodioside successively, and sodium chloride, stirring is to dissolve fully, adds the injection water to scale, filtering with microporous membrane, potting, 121 ℃ of sterilization 30min are promptly.
The present invention has overcome the deficiencies in the prior art, and by investigating of comprehensive science, the perfect high-purity of a cover of creationary foundation is extracted the method for rhodioside composition; The present invention is directed to this blank of salidroside injection preparation that lacks high bioavailability in the market simultaneously, in conjunction with the inherent peculiar property of Radix Rhodiolae, by the adjuvant of a large amount of infusion preparations comprehensively being investigated and the detailed research of all kinds of ejection preparation preparation parameters the creationary preparation technology who sets up gadol injection, injectable powder, great transfusion preparation.Adopt the prepared salidroside injection preparation of the present invention to have following advantage:
1. the purity height of the rhodioside that the present invention extracted reaches more than 90%, makes it in treatment cardiovascular and cerebrovascular disease good effect, instant effect, and the bioavailability height, and have tangible safe without toxic side effect.
2. the prepared injection class preparation of the present invention is rapid-action, can control the cardiovascular and cerebrovascular disease state of an illness rapidly, helps rescuing; Avoided the liver first-pass effect, the bioavailability height, therapeutic effect is good.
3. the inventive method is simple, and is easy and simple to handle convenient, is suitable for the needs of industrialized great production.
Specific embodiment 1:
The rhodioside aqueous injection: Radix Rhodiolae crude extract temperature is 30 ℃ a water stirring and dissolving 30 minutes, filters and obtains clear filtrate, regulates filtrate pH value=3; Adsorb with adsorbent resin under the room temperature, use 10% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 5% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 40 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separate out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 50 ℃ of dryings obtain purity and are 97.5% rhodioside goods, take by weighing rhodioside 0.2%, sodium chloride 0.5%, chlorobutanol 0.2% places dispensing containers, the water for injection that adds configuration amount 80%, stirring is to dissolve fully, adds the injection water to scale, filtering with microporous membrane, potting is in the ampoule of 2ml, and 100 ℃ of sterilization 30min promptly.
Specific embodiment 2:
The rhodioside aqueous injection: Radix Rhodiolae crude extract temperature is 40 ℃ a water stirring and dissolving 40 minutes, filters and obtains clear filtrate; Regulate filtrate pH value=3, adsorb with adsorbent resin under the room temperature, use 30% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 10% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 30 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separate out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 60 ℃ of dryings obtain purity and are 98% rhodioside goods, take by weighing rhodioside 2%, sodium chloride 1.5%, chlorobutanol 0.5% places dispensing containers, the water for injection that adds configuration amount 80%, stirring is to dissolve fully, adds the injection water to scale, filtering with microporous membrane, potting is in the ampoule of 2ml, and 100 ℃ of sterilization 30min promptly.
Specific embodiment 3:
The rhodioside injectable powder: Radix Rhodiolae crude extract temperature is 50 ℃ a water stirring and dissolving 1 hour, filters and obtains clear filtrate; Regulate filtrate pH value=3, adsorb with adsorbent resin under the room temperature, use 25% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 15% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 30 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 40 ℃ of dryings obtain purity and are 95% rhodioside goods, take by weighing rhodioside 5%, mannitol 10%, and place sterile chamber, and add an amount of water for injection dissolving, make material solution, percent by volume is 0.1% to add active carbon in material solution by weight, filters; The filtrate water-bath is heated to 55 ℃, at 10 ℃, places 20 minutes, adds water for injection again to proportion of composing, regulates PH about about 6, uses filtering with microporous membrane, is sub-packed in the cillin bottle false add butyl rubber plug; Place freeze drying box to be cooled to-40 ℃ cillin bottle, kept 4 hours, be evacuated to-300mmHg, the dividing plate heating makes temperature rise to-15 ℃, keeps 4 hours, is warming up to 20 ℃, is incubated 5 hours, and vacuum is promptly made Hongjingtian glycoside freeze dried powder injection agent after rolling lid.Specific embodiment 4:
The rhodioside injectable powder:
Radix Rhodiolae crude extract temperature is 60 ℃ a water stirring and dissolving 2 hours, filters and obtains clear filtrate; Regulate filtrate pH value=3.5, adsorb with adsorbent resin under the room temperature, use 35% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 20% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 50 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 55 ℃ of dryings obtain purity and are 97.8% rhodioside goods, take by weighing rhodioside 0.2%, dextran 2%, and place sterile chamber, and add an amount of water for injection dissolving, make material solution, percent by volume is 0.5% to add active carbon in material solution by weight, filters; The filtrate water-bath is heated to 65 ℃, at 60 ℃, places 60 minutes, adds water for injection again to proportion of composing, regulates PH about about 6, uses filtering with microporous membrane, is sub-packed in the cillin bottle false add butyl rubber plug; Place freeze drying box to be cooled to 45 ℃ cillin bottle, kept 6 hours, be evacuated to-300～600mmHg, the dividing plate heating makes temperature rise to-19 ℃, keeps 6 hours, is warming up to 30 ℃, is incubated 7 hours, and vacuum is promptly made Hongjingtian glycoside freeze dried powder injection agent after rolling lid.
Specific embodiment 5:
The rhodioside bulk capacity injection: Radix Rhodiolae crude extract temperature is 70 ℃ a water stirring and dissolving 20 minutes, filters and obtains clear filtrate; Regulate filtrate pH value=4, adsorb with adsorbent resin under the room temperature, use 40% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 25% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 60 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 65 ℃ of dryings obtain purity and are 98.5% rhodioside goods, take by weighing rhodioside 10%, sodium chloride 1.5% places dispensing containers, the water for injection that adds configuration amount 80%, stirring are to dissolve fully, add the injection water to scale, filtering with microporous membrane, potting, 121 ℃ of sterilization 30min are promptly
Specific embodiment 6:
The rhodioside bulk capacity injection: Radix Rhodiolae crude extract temperature is 80 ℃ a water stirring and dissolving 70 minutes, filters and obtains clear filtrate; Regulate filtrate pH value=4.5, adsorb with adsorbent resin under the room temperature, use 30% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 5% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 20 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 70 ℃, obtain purity and be 99% rhodioside goods, take by weighing rhodioside 0.2%, sodium chloride 0.5% places dispensing containers, adds the water for injection of configuration amount 80%, stirring is to dissolve fully, adds the injection water to scale, filtering with microporous membrane, potting, 121 ℃ of sterilization 30min promptly.
Specific embodiment 7:
Rhodioside injectable powder: rhodioside aqueous injection: Radix Rhodiolae crude extract temperature is 90 ℃ a water stirring and dissolving 3 hours, filters and obtains clear filtrate; Regulate filtrate pH value=5, adsorb with adsorbent resin under the room temperature, use 15% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 10% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, 45 ℃ of heating-up temperatures, filtered while hot, thickening filtration is cooled to 0 ℃, separate out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, 35 ℃ of dryings obtain purity and are 98% rhodioside goods. take by weighing rhodioside 2%, lactose 5%, water for injection 78.0%～97.0%., and place sterile chamber, add an amount of water for injection dissolving, make material solution, percent by volume is 0.2% to add active carbon in material solution by weight, filters; The filtrate water-bath is heated to 62 ℃, at 40 ℃, places 30 minutes, adds water for injection again to proportion of composing, regulates PH about about 6, uses filtering with microporous membrane, is sub-packed in the cillin bottle false add butyl rubber plug; Place freeze drying box to be cooled to-43 ℃ cillin bottle, kept 5 hours, be evacuated to-400mmHg, the dividing plate heating makes temperature rise to-17 ℃, keeps 5 hours, is warming up to 25 ℃, is incubated 6 hours, and vacuum is promptly made Hongjingtian glycoside freeze dried powder injection agent after rolling lid.
Claims (10)
1, a kind of high-purity salidroside injection preparation and preparation technology thereof is characterized in that comprising highly purified rhodioside and preparation technology thereof, and this highly purified rhodioside and adjuvant dispose the ejection preparation that forms by a certain percentage.
2, preparation according to claim 1 is characterized in that: the purity of described highly purified rhodioside is more than 90%.
3, the described ejection preparation of claim 1 is characterized in that described preparation comprises: liquid drugs injection, powder pin, bulk capacity injection preparation.
4, according to claim 1, the preparation technology of 2 high-purity salidrosides of stating is characterized in that: Radix Rhodiolae crude extract temperature is 20～90 ℃ a water stirring and dissolving 20 minutes～3 hours, filters and obtains clear filtrate; Regulate filtrate pH value=1～5, adsorb with adsorbent resin under the room temperature, use 10%～40% acetone, low-carbon alcohols or acetate esters organic solvent eluting then; Concentrate eluant is a syrupy shape, dries behind the adding adsorbent resin mix homogeneously; Adsorbent resin after the oven dry is joined in the chromatographic column of having filled adsorbent resin, and the low pure carbon class organic solvent with 5%～25% under the room temperature carries out chromatography, collects the chromatography permeate, with its concentrated evaporate to dryness; Add low-carbon alcohols or acetate esters organic solvent heating for dissolving, heating-up temperature is no more than 60 ℃, filtered while hot, thickening filtration is cooled to 0 ℃, separates out crystallization, wash crystalline substance with low-carbon alcohols or acetate esters organic solvent, dry below 70 ℃, obtain highly purified rhodioside goods, the purity of these rhodioside goods reaches more than 90%.
5, according to claim 1, the hydro-acupuncture preparation of 3 described high-purity salidrosides, it is characterized in that: in dispensing containers, add proper amount of water for injection, add high-purity salidroside 0.5%～1.5% successively, sodium chloride 0.7%～1.2%, chlorobutanol 0.2%～0.4%, stirring and dissolving adds the injection water, filter, potting is in ampoule, and sterilization promptly gets it by high-purity salidroside 0.2%～10%, sodium chloride 0.5%～1.5%, chlorobutanol 0.2%～0.5%, surplus are water for injection.
6, the preparation of aqueous injection according to claim 5 is characterized in that its composition weight proportion is excellent and is high-purity salidroside 0.5%～1.5%, sodium chloride 0.7%～1.2%, and chlorobutanol 0.2%～0.4%, surplus is a water for injection.
7, according to claim 1, the powder injection formulation of 3 described high-purity salidrosides, it is characterized in that: take by weighing high-purity salidroside 0.2%～10%, mannitol or dextran or lactose 2%～10%, and place sterile chamber, and add an amount of water for injection dissolving, make material solution, percent by volume is 0.1%～0.5% to add active carbon in material solution by weight, filters; The filtrate water-bath is heated to 55 ℃～65 ℃, at 10 ℃～60 ℃, places 20～60 minutes, adds water for injection more eventually to 78.0%～97.0%., regulate PH about about 6, use filtering with microporous membrane, be sub-packed in the cillin bottle false add butyl rubber plug; Place freeze drying box to be cooled to-40 ℃～-45 ℃ cillin bottle, kept 4～6 hours, be evacuated to-300～600mmHg, the dividing plate heating, make temperature rise to-15 ℃～-19 ℃, kept 4～6 hours, be warming up to 20～30 ℃, be incubated 5～7 hours, vacuum is promptly made Hongjingtian glycoside freeze dried powder injection agent after rolling lid.
8, rhodioside injectable powder according to claim 7 is characterized in that its composition weight proportion is excellent and is: high-purity salidroside 1%～8%, mannitol or dextran or lactose 4%～8%, water for injection consumption are 90.0%～95.8%.
9, according to claim 1, the bulk capacity injection of 3 described high-purity salidrosides, it is characterized in that: take by weighing high-purity salidroside 0.2%～10% in proportion, sodium chloride 0.5%～1.5% places dispensing containers, the water for injection that adds configuration amount 80%, stirring is to dissolve fully, adds the injection water to scale, filtering with microporous membrane, potting, 121 ℃ of sterilization 30min promptly.
10, the rhodioside bulk capacity injection stated of claim 9 is characterized in that its composition weight proportion is excellent and is: high-purity salidroside 0.5%～1.5%, and sodium chloride 0.7%～1.2%, surplus is a water for injection.
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| CNA2008100444365A CN101584655A (en) | 2008-05-23 | 2008-05-23 | High-purity salidroside injection preparation and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351416A (en) * | 2013-08-02 | 2013-10-16 | 福建中医药大学 | New crystal form A of salidroside and preparation method thereof |
| CN104017036A (en) * | 2014-06-11 | 2014-09-03 | 福建中医药大学 | Novel crystal form of rhodioloside and preparation method of novel crystal form |
| CN104435057A (en) * | 2014-12-19 | 2015-03-25 | 通化玉圣药业有限公司 | Preparation method of rhodiola rosea injection by low-temperature decarbonization |
| CN110403949A (en) * | 2019-08-21 | 2019-11-05 | 天津龙尔达科技发展有限公司 | Application of salidroside in preparation of medicine for treating pulmonary fibrosis diseases |
-
2008
- 2008-05-23 CN CNA2008100444365A patent/CN101584655A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351416A (en) * | 2013-08-02 | 2013-10-16 | 福建中医药大学 | New crystal form A of salidroside and preparation method thereof |
| CN104017036A (en) * | 2014-06-11 | 2014-09-03 | 福建中医药大学 | Novel crystal form of rhodioloside and preparation method of novel crystal form |
| CN104435057A (en) * | 2014-12-19 | 2015-03-25 | 通化玉圣药业有限公司 | Preparation method of rhodiola rosea injection by low-temperature decarbonization |
| CN110403949A (en) * | 2019-08-21 | 2019-11-05 | 天津龙尔达科技发展有限公司 | Application of salidroside in preparation of medicine for treating pulmonary fibrosis diseases |
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