CN101570791A - Special primer for identifying which variety of Glu-A3 protein subunit is carried in wheat as well as application thereof - Google Patents

Abstract

The invention discloses a special primer for identifying which type of Glu-A3 protein subunit is carried in wheat as well as an application thereof. The provided special primer comprises at least one pair of the following seven pairs of primers: the primer pair constituted by nucleotides shown in sequence 18 and 20; the primer pair constituted by the nucleotides shown in sequence 19 and 21; the primer pair constituted by the nucleotides shown in sequence 18 and 22; the primer pair constituted by the nucleotides shown in sequence 19 and 23; the primer pair constituted by the nucleotides shown in sequence 18 and 24; the primer pair constituted by the nucleotides shown in sequence 18 and 25; and the primer pair constituted by the nucleotides shown in sequence 18 and 26. The utilization of the special primer, a reagent box or a method of the invention can screen the variety of the wheat at the early stage of development, thereby optimizing the hybrid combination, accelerating the breeding speed and reducing the breeding cost. The method has simple operation, stability, reliability and low cost, thereby being applicable to popularization and having important significance for researching the related characters of the wheat quality and improving the wheat quality. The invention will play an important role in wheat quality breeding.

Description

Differentiate that wheat is the primer special and the application thereof of carrying which kind of Glu-A3 protein protomer kind

Technical field

The present invention relates to differentiate that wheat is the primer special and the application thereof of carrying which kind of Glu-A3 protein protomer kind.

Background technology

The wheat storage protein mainly comprises glutenin and prolamine.The difference of the two is that the former can form multichained construction by intermolecular disulfide bond, and the latter then exists with monomer subunit form.Glutenin accounts for 35～45% of seed storage protein, is one of main component of gluten.The peptide interchain disulfide linkage and the intramolecular disulfide linkage of glutenin can mutually combine, and its protein mostly is the polare Aminosaeren composition in addition, very easily forms macromole aggregate (Polymer), makes dough have certain elasticity.Good elasticity and ductility are the bases that makes high quality bread, so selection contains good HMW-GS and LMW-GS is one of major objective of marker assisted selection in the Wheat Quality Improvement.According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Sodium-dodecyl-sulphatepolyacrylamide gel electrophoresis, SDS-PAGE) mobility, glutenin can be divided into hmw glutenin subunit (High-molecular-weight glutenin subunits, HMW-GS) and low molecular weight glutenin subunit (Low-molecular-weight glutenin subunits, LMW-GS).HMW-GS is respectively by the genes encoding that is positioned at the first homology cohort karyomit(e) long-armed Glu-A1, Glu-B1 and Glu-D1 site (general designation Glu-1), LMW-GS is then respectively by genes encoding (Payne etc., 1987 of the Glu-A3 that is positioned at galianconism, Glu-B3 and Glu-D3 site (general designation Glu-3); Singh and Shepherd, 1988).Effective choice need be based upon on the basis to the evaluation of different subunits and evaluation, to satisfy different breeding objectives.

Because the LMW-GS number is more, molecular weight is little and overlapped with a large amount of prolamines on electrophoretogram, traditional unidirectional SDS-PAGE is difficult to it is distinguished.After Singh and Shepherd (1988) had developed two step SDS-PAGE methods, this problem was just solved largely.In ensuing research, RPLC (Reversed-phase high performance liqnid chromatography, RP-HPLC) and capillary vessel electrophoretic technique (Capillary electrophoresis, CE) also be used to differentiate different low molecular weight glutenin subunits (Bean and Lookhart, 2000), but since these technology comparatively complexity or application charges are higher, all not by large-scale application.Therefore to LMW-GS and and Q factor between the research that concerns fall behind more than HMW-GS.

Have confidential relation between LMW-GS and the wheat quality, the influence of different subunits also is not quite similar.Payne etc. (1984) connect the quality trait of low molecular weight glutenin subunit and tetraploid for the first time.Branlard and Felix (1994) think that HMW-GS can explain 18%～55% of gluten strength and visco-elasticity variation, and LMW-GS can explain 20% of its variation.Thereby, when the concerning of research glutenin and wheat quality, should take all factors into consideration the effect of HMW-GS and LMW-GS.Cornish etc. (1993) studies show that, subunit combinations is that the ductility of kind of 2*, 7+8,2+12, Glu-A3b, Glu-B3b and Glu-D3b is best.Also have some researchs to think, for different Glu-3 subunits, the kind tool that is combined as Glu-A3b, Glu-B3b and Glu-D3b is best ductility, subunit combinations is that the extensibility of kind of Glu-A3b, Glu-B3b and Glu-D3c is also better, subunit combinations is Glu-A3b, Glu-B3b and Glu-D3b, Glu-A3b, Glu-B3b and Glu-D3c, and the kind of Glu-A3c, Glu-B3b and Glu-D3c, its bread quality is best.(2005) such as He etc. (2005) and Liu have done more detailed research to Chinese wheat breed Glu-1 and Glu-3 site allelic variation, the relation of clear and definite Glu-A3 and Glu-B3 and bread and noodle quality, think the d in Glu-B3 site and b allelotrope to the effect of dough ductility greater than other allelotrope, Glu-A3d and Glu-B3d are a bit larger tham other allelotrope to the contribution of Chinese extra dry white wine noodle quality.

Molecule marker is the designed a kind of genetic marker of utilizing with objective trait close linkage or relevant gene of polymorphism.Molecule marker is not subjected to genetic effect and Effect of Environmental, and is lower to technical requirements, thereby the result is reliable, and can select in generation morning, thereby shortens breeding cycle greatly.Lack easy, effective authentication method, limited analysis and utilization LMW-GS.Thereby the relevant mark of exploitation LMW-GS is for carrying out the LMW-GS functional study, and molecular marker assisted selection (Marker-assistant selection MAS) has significance.

Summary of the invention

The purpose of this invention is to provide and differentiate that wheat is the primer special and the application thereof of carrying which kind of Glu-A3 protein protomer kind.

Discriminating wheat provided by the invention is the primer special that carries the wheat breed of which kind of Glu-A3 protein protomer, comprises in following seven pairs of primers at least one pair of:

(1) primer formed of Nucleotide shown in the sequence 20 of Nucleotide and sequence table shown in the sequence 18 of sequence table is to (gluA3a primer to);

(2) primer formed of Nucleotide shown in the sequence 21 of Nucleotide and sequence table shown in the sequence 19 of sequence table is to (gluA3b primer to);

(3) primer formed of Nucleotide shown in the sequence 22 of Nucleotide and sequence table shown in the sequence 18 of sequence table is to (gluA3ac primer to);

(4) primer formed of Nucleotide shown in the sequence 23 of Nucleotide and sequence table shown in the sequence 19 of sequence table is to (gluA3d primer to);

(5) primer formed of Nucleotide shown in the sequence 24 of Nucleotide and sequence table shown in the sequence 18 of sequence table is to (gluA3e primer to);

(6) primer formed of Nucleotide shown in the sequence 25 of Nucleotide and sequence table shown in the sequence 18 of sequence table is to (gluA3f primer to);

(7) primer formed of Nucleotide shown in the sequence 26 of Nucleotide and sequence table shown in the sequence 18 of sequence table is to (gluA3g primer to).

Described primer special also can be made up of described seven pairs of primers.

Described primer special can be applicable to prepare differentiates that wheat is the test kit that carries the wheat breed of which kind of Glu-A3 protein protomer.

It is the test kit that carries the wheat breed of which kind of Glu-A3 protein protomer that the present invention also protects a kind of wheat of differentiating, comprises described primer special.

The present invention also protects and a kind ofly differentiates that wheat to be measured is the method for carrying the wheat breed of which kind of Glu-A3 protein protomer, comprises the steps:

1) genomic dna with wheat to be measured is a template, carries out pcr amplification with described primer special;

2) detect pcr amplification product, judge that by the size of pcr amplification product wheat to be measured is the wheat breed that carries which kind of Glu-A3 protein protomer.

Judging criterion is as follows: the genome with wheat to be measured is a template, adopts the gluA3a primer to carrying out PCR, if obtain the amplified production of 529bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3a.Genome with wheat to be measured is a template, adopt the gluA3ac primer to carrying out PCR, if obtain the amplified production of 573bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3a or Glu-A3c, the PCR result associating right with the gluA3a primer, if use the Glu-A3a primer to not obtaining the amplified production of 529bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3c.Genome with wheat to be measured is a template, adopts the gluA3b primer to carrying out PCR, if obtain the amplified production of 894bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3b.Genome with wheat to be measured is a template, adopts the gluA3d primer to carrying out PCR, if obtain the amplified production of 967bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3d.Genome with wheat to be measured is a template, adopts the gluA3e primer to carrying out PCR, if obtain the amplified production of 158bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3e.Genome with wheat to be measured is a template, adopts the gluA3f primer to carrying out PCR, if obtain the amplified production of 552bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3f.Genome with wheat to be measured is a template, adopts the gluA3g primer to carrying out PCR, if obtain the amplified production of 1345bp, the Glu-A3 protein protomer of described wheat to be measured is Glu-A3g.

Described primer special, described test kit and described method all can be applicable to wheat breeding.

Described wheat specifically can be common wheat.

Utilize primer special of the present invention, test kit or method growing the early screening wheat breed, thereby optimize cross combination, accelerate breeding speed, reduce the breeding cost.Method provided by the invention is simple to operate, reliable and stable, with low cost, is suitable for applying, and is significant to research wheat quality correlated character and improvement wheat quality.The present invention will play a significant role in Wheat Breeding for Quality.

Description of drawings

Fig. 1 is the pcr amplification detected result of gluA3a primer to 7 materials in the his-and-hers watches 1.

Fig. 2 is the pcr amplification detected result of gluA3b primer to 7 materials in the his-and-hers watches 1.

Fig. 3 is the pcr amplification detected result of gluA3ac primer to 7 materials in the his-and-hers watches 1.

Fig. 4 is the pcr amplification detected result of gluA3d primer to 7 materials in the his-and-hers watches 1.

Fig. 5 is the pcr amplification detected result of gluA3e primer to 7 materials in the his-and-hers watches 1.

Fig. 6 is the pcr amplification detected result of gluA3f primer to 7 materials in the his-and-hers watches 1.

Fig. 7 is the pcr amplification detected result of gluA3g primer to 7 materials in the his-and-hers watches 1.

Fig. 8 is SDS-PAGE test section material Glu-A3 subunit result; Wherein represents the purpose band.

Embodiment

Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is ordinary method.Used test materials among the following embodiment if no special instructions, is to buy from routine biochemistry reagent shop and obtains.Primer among the following embodiment is synthetic to be finished by the living worker in Shanghai with dna sequencing.The wheat lines that all are used in following examples is all preserved the center available from country of Chinese Academy of Agricultural Sciences farm crop germplasm.

The acquisition of embodiment 1, common wheat low molecular weight glutenin Glu-A3 locus gene

Used wheat breed sees Table 1.

The wheat breed that table 1 is used to clone the Glu-A3 locus gene and analyzes Glu-A3 subunit specific mark

Seven wheat breeds in the table 1 are wheat (the Vawser MJ that contains definite Glu-A3 protein protomer, Cornish GB, Shepherd KW (2002) Rheological dough properties of Aroonaisolines differing in glutenin subunit composition.In:Cereals 2002, Proceedings of the 52nd Australian Cereal Chemistry Conference.Christchurch, New Zealand. (Eds CK Black, JF Panozzo, CW Wrigley, IL Bateyand N Larsen) pp.53-58. (Cereal Chemistry Division, Royal AustralianChemical Institute)).

Go up Glu-A3 site correlated series (GENBANK ACCESSION NO.EU189087, AB062868, FJ441117 and EF426565) according to GenBank, it is as follows to design 3 pairs of primers:

Primer is to first: P1 (upstream primer): 5 '-AAACAGAATTATTAAAGCCGG-3 ';

P2 (downstream primer): 5 '-CAGGCAAGACAAACTTATGAA-3 ';

Primer pair B:

P3 (upstream primer): 5 '-CATCTCTGATCCATCCAAACAT-3 ';

P4 (downstream primer): 5 '-GTGATCCAACTATATATTACTAGAC-3 ';

Primer is to third:

P5 (upstream primer): 5 '-TTCAGATGCAGCCAAACAA-3 ';

P6 (downstream primer): 5 '-CAAACTTATGAACTTGCAAGG-3 '.

Utilize the genomic dna of the wheat in above-mentioned 3 pairs of primer his-and-hers watches 1 to carry out pcr amplification respectively.

In the pcr amplification, the PCR reaction system of per 20 μ l is as follows: the template DNA of 50ng, Taq enzyme 1U (TaKaRa), upstream and downstream primer (5 μ molL ^-1) each 1.2 μ l, dNTP (2.5mmolL ^-1, TaKaRa) 0.8 μ l, 10 * PCR damping fluid, 2 μ l are with sterile distilled water postreaction system to 20 μ l.

PCR response procedures: 94 ℃ of 5min; 94 ℃ of 35s then, 57 ℃ (first and second pairs of primers) or 58 ℃ (the 3rd pair of primer) annealing 35s, 72 ℃ are extended 1min 30s, totally 38 circulations; Last 72 ℃ of 5min, 4 ℃ of insulations.

Primer obtains a Glu-A3 locus gene, called after GluA3-1 to the pcr amplification product order-checking back of first.GluA3-1 comprises 7 allelic variations (seeing the sequence 7 of the sequence 1 of sequence table to sequence table) altogether.

Obtained a Glu-A3 locus gene, called after GluA3-2 after the pcr amplification product order-checking of primer pair B.GluA3-2 comprises totally 4 allelic variations (seeing the sequence 11 of the sequence 8 of sequence table to sequence table) altogether.

Primer has obtained a Glu-A3 locus gene, called after GluA3-3 after to the order-checking of third pcr amplification product.GluA3-3 comprises 6 allelic variations (seeing the sequence 17 of the sequence 12 of sequence table to sequence table) altogether.

The acquisition of embodiment 2, common wheat low molecular weight glutenin Glu-A3 subunit specific mark

According to 3 Glu-A3 genes of above-mentioned acquisition and the corresponding relation between the Glu-A3 protein protomer, right behind the sequence alignment according to the Auele Specific Primers of 7 pairs of identification of the difference design Glu-A3 protein protomers between the allele variation, see Table 2.

Table 2 differentiates that the Auele Specific Primer of Glu-A3 protein protomer is right

Utilize the genomic dna of the wheat in above-mentioned 7 pairs of primer his-and-hers watches 1 to carry out pcr amplification respectively.

The PCR reaction system is as follows: the template DNA of 50ng, Taq enzyme 1U (TaKaRa), upstream and downstream primer (5 μ molL ^-1) each 1.2 μ l, dNTP (2.5mmolL ^-1, TaKaRa) 0.8 μ l, 10 * PCR damping fluid, 2 μ l are with sterile distilled water postreaction system to 20 μ l.

PCR response procedures: 94 ℃ of 5min; 94 ℃ of 35s then, 60 ℃ of annealing 35s, 72 ℃ are extended 1min30s, totally 38 circulations; Last 72 ℃ of 5min, 4 ℃ of insulations.

After the PCR reaction finishes, amplified production is carried out 1.5% agarose gel analysis, 200V voltage electrophoresis 20 minutes, (EB), 0.5% ethidium bromide (EB) dyeing is 10 minutes then, after the distilled water rinsing on Gel Doc XRSystem imaging system with the ultraviolet lamp scanning imagery and deposit computer in.

The detected result that adopts gluA3a to carry out the product of pcr amplification is seen Fig. 1.Among Fig. 1, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the Glu-A3a protein protomer shows the special band of a 529bp.The detected result that adopts gluA3b to carry out the product of pcr amplification is seen Fig. 2.Among Fig. 2, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the Glu-A3b protein protomer shows the special band of a 894bp.The detected result that adopts gluA3ac to carry out the product of pcr amplification is seen Fig. 3.Among Fig. 3, M: molecular weight standard; 1-7: sample 1-7.The wheat sample standard deviation that contains Glu-A3a and Glu-A3c protein protomer shows the special band of a 573bp.The detected result that adopts gluA3d to carry out the product of pcr amplification is seen Fig. 4.Among Fig. 4, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the gluA3d protein protomer shows the special band of a 967bp.The detected result that adopts gluA3e to carry out the product of pcr amplification is seen Fig. 5.Among Fig. 5, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the gluA3e protein protomer shows the special band of a 158bp.The detected result that adopts gluA3f to carry out the product of pcr amplification is seen Fig. 6.Among Fig. 6, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the gluA3f protein protomer shows the special band of a 552bp.The detected result that adopts gluA3g to carry out the product of pcr amplification is seen Fig. 7.Among Fig. 7, M: molecular weight standard; 1-7: sample 1-7.The wheat sample that contains the gluA3g protein protomer shows the special band of a 1345bp.

Embodiment 3, seven pairs of application that Auele Specific Primer is right and result verification

For further verifying 7 pairs of practicality that Auele Specific Primer is right of exploitation, utilize 7 materials (numbering 1-7) that are used for clone gene among 121 parts of wheat lines of 7 pairs of primers detections (numbering 8-128) and the embodiment 1, the results are shown in Table 3.The Glu-A3 site protein protomer of these materials is analyzed through SDS-PAGE, thereby can verify 7 pairs of detected results that Auele Specific Primer is right.Fig. 8 is SDS-PAGE test section material Glu-A3, Glu-B3 and Glu-D3 subunit result.Among Fig. 8,1:21; 2:26; 3:30; 4:Pav ó n (contrast); 5:8; 6:11; 7:17; 8:19; 9:31; 10:90; 11:18; 12:24; 13:25; 14:0pata (contrast); Above numeral all refers to the numbering in the table 3.Contrast is for the reference position of respective strap is provided.

The checking of the special STS mark of table 3Glu-A3 protein protomer

Found that, in 128 parts of materials, the detected result unanimity of two kinds of methods.

Sequence table

＜110〉Institute of Crop Science, Chinese Academy of Agricultural Science

＜120〉differentiate that wheat is the primer special and the application thereof of carrying which kind of Glu-A3 protein protomer kind

<130>CGGNARY92315

<160>26

<210>1

<211>1501

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>1

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaaac?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aacaaccacc?attttcacag?caacaacaac 240

caccattttc?gcagcaacag?caatcaccat?tttcgcaaca?acaacaacaa?ccaccatttt 300

tgcagcaaca?acaaccaccg?ttttcacaac?agccaccaat?ttcacagcag?caacaaccac 360

cattttcaca?gcaacaacaa?ccacaatttt?cacagcaaca?gcagccacca?tattcgcagc 420

aacaacagcc?accatattcg?cagcaacaac?aaccaccatt?ttcgcagcaa?cagcaaccac 480

cattttcgca?gcaacaacaa?ccaccatttt?tgcagcaaca?acaacaacca?ccatttacac 540

agcaacaaca?accatcgttt?tcacaacagc?caccaatttc?acagcagcag?cagcagcaac 600

aacaacaaca?acaaccattt?acacagcaac?aacaaccacc?attttcacaa?cagccaccaa 660

tttcacagca?gcaacaacca?ccattttcgc?agcaacaaca?accaccattt?tcacagcaac 720

aacaaatacc?agttattcat?ccatctgttt?tgcagcagct?aaacccatgc?aaggtattcc 780

tccagcagca?gtgcatccct?gtggcaatgc?agcgatgtct?tgctaggtca?caaatgttgc 840

agcagagcat?ttgccatgtg?atgcagcaac?aatgttgcca?gcagttgcgg?caaatccccg 900

agcaatcccg?ccatgagtca?atccgtgcta?tcatctactc?tatcatcctg?cagcagcagc 960

agcagcaaca?acaacaacaa?caacagggtc?agagtatcat?ccaatatcag?caacaacaac 1020

cccaacagtt?gggccaatgt?gtctcccaac?cccaacagca?gttgcagcag?caactcgggc 1080

aacaacctca?acaacaacaa?ttggcacatg?gtaccttttt?gcagccacac?cagatagctc 1140

agcttgaggt?gatgacttcc?attgcactcc?gtaccctgcc?aacaatgtgc?agtgtcaatg 1200

tgccgttgta?cgaaaccacc?actagtgtgc?cattaggcgt?tggcatcgga?gttggtgtct 1260

actgataaga?aaagatctct?agtaatatat?agttggatca?ccgttgttta?gtcgatggat 1320

atgtcaatgt?agcggtgaca?aataaagtgt?cacacaacgt?catgtgtgac?ccgctcaaac 1380

tagttgttta?aattctgaaa?taaaatacaa?ataaaggtgt?attaagaaaa?tgttcatatc 1440

ggcattgtgt?ggatgtcgat?ctgattgcca?tgcatgcaag?ttcataagtt?tgtcttgcct 1500

g 1501

<210>2

<211>1525

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>2

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaaac?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aaccaccacc?attttcgcag?caacaacaac 240

caccattttc?acagcaacaa?caaccaccat?tttcgcagca?acagcaatca?ccattttcgc 300

aacaacaaca?acaaccacca?tttttgcagc?aacaacaacc?accgttttca?caacaaccac 360

caatttcaca?gcagcaacaa?ccaccatttt?cacagcaaca?acaaccacaa?ttttcacagc 420

aacagcagcc?accatattcg?cagcaacaac?aaccaccata?ttcgcagcaa?caacaaccac 480

cattttcgca?gcaacagcaa?ccaccatttt?cgcagcaaca?acaaccacca?ttttcgcagc 540

aacaacaaca?accaccattt?acacagcaac?aacaaccacc?gttttcacaa?cagccaccaa 600

tttcacagca?gcagcaacag?caacaacaac?aacaacaacc?atttacacag?caacaacaac 660

caccgttttc?acaacagcca?ccaatttcac?agcagcaaca?accaccattt?tcgcagcaac 720

aacaaccacc?attttcacag?caacaacaaa?taccagttat?tcatccatct?gttttgcagc 780

agctaaaccc?atgcaaggta?ttcctccagc?agcagtgcat?ccctgtggca?atgcagcgat 840

gtcttgctag?gtcacaaatg?ttgcagcaga?gcatttgcca?tgtgatgcag?caacaatgtt 900

gccagcagtt?gcggcaaatc?cccgagcaat?cccgccatga?gtcaatccgt?gctatcatct 960

actctatcat?cctgcagcag?cagcagcagc?aacaacaaca?acaacaacag?ggtcagagta 1020

tcatccaata?tcagcaacaa?caaccccaac?agttgggcca?atgtgtctcc?caaccccaac 1080

agcagttgca?gcagcaactc?gggcaacaac?ctcaacaaca?acaattggca?catggtacct 1140

ttttgcagcc?acaccagata?gctcagcttg?aggtgatgac?ttccattgca?ctccgtaccc 1200

tgccaacaat?gtgcagtgtc?aatgtgccgt?tgtacgaaac?caccactagt?gtgccattag 1260

gcgttggcat?cggagttggt?gtctactgat?aagaaaagat?ctctagtaat?atatagttgg 1320

atcaccgttg?tttagtcgat?ggatatgtca?atgtagcggt?gacaaataaa?gtgtcacaca 1380

acgtcatgtg?tgacccgctc?aaactagttg?tttaaattct?gaaataaaat?acaaataaag 1440

gtgtattaag?aaaatgttca?tatcggcatt?gtgtggatgt?cgatctgatt?gccatgcatg 1500

caagttcata?agtttgtctt?gcctg 1525

<210>3

<211>1501

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>3

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaaac?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aacaaccacc?attttcacag?caacaacaac 240

caccattttc?gcagcaacag?caatcaccat?tttcgcaaca?acaacaacaa?ccaccatttt 300

tgcagcaaca?acaaccaccg?ttttcacaac?agccaccaat?ttcacagcag?caacaaccac 360

cattttcaca?gcaacaacaa?ccacaatttt?cacagcaaca?gcagccacca?tattcgcagc 420

aacaacagcc?accatattcg?cagcaacaac?aaccaccatt?ttcgcagcaa?cagcaaccac 480

cattttcgca?gcaacaacaa?ccaccatttt?cgcagcaaca?acaacaacca?ccatttacac 540

agcaacaaca?accatcgttt?tcacaacagc?caccaatttc?acagcagcag?cagcagcaac 600

aacaacaaca?acaaccattt?acacagcaac?aacaaccacc?attttcacaa?cagccaccaa 660

tttcacagca?gcaacaacca?ccattttcgc?agcaacaaca?accaccattt?tcacagcaac 720

aacaaatacc?agttattcat?ccatctgttt?tgcagcagct?aaacccatgc?aaggtattcc 780

tccagcagca?gtgcatccct?gtggcaatgc?agcgatgtct?tgctaggtca?caaatgttgc 840

agcagagcat?ttgccatgtg?atgcagcaac?aatgttgcca?gcagttgcgg?caaatccccg 900

agcaatcccg?ccatgagtca?atccgtgcta?tcatctactc?tatcatcctg?cagcagcagc 960

agcagcaaca?acaacaacaa?caacagggtc?agagtatcat?ccaatatcag?caacaacaac 1020

cccaacagtt?gggccaatgt?gtctcccaac?cccaacagca?gttgcagcag?caactcgggc 1080

aacaacctca?acaacaacaa?ttggcacatg?gtaccttttt?gcagccacac?cagatagctc 1140

agcttgaggt?gatgacttcc?attgcactcc?gtaccctgcc?aacaatgtgc?agtgtcaatg 1200

tgccgttgta?cgaaaccacc?actagtgtgc?cattaggcgt?tggcatcgga?gttggtgtct 1260

actgataaga?aaagatctct?agtaatatat?agttggatca?ccgttgttta?gtcgatggat 1320

atgtcaatgt?agcggtgaca?aataaagtgt?cacacaacgt?catgtgtgac?ccgctcaaac 1380

tagttgttta?aattctgaaa?taaaatacaa?ataaaggtgt?attaagaaaa?tgttcatatc 1440

ggcattgtgt?ggatgtcgat?ctgattgcca?tgcatgcaag?ttcataagtt?tgtcttgcct 1500

g 1501

<210>4

<211>1443

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>4

aaacagaatt?attaaagccg?gtgcaaaaaa?ggaaaagagg?tggtgttccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaagc?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcga 180

gtgccgttgc?gcaaatttca?cagcaacaac?aaccaccact?attttcgcag?caacaacaac 240

caccattttc?acagcaacaa?caaccaccat?tttcgcagca?acagcaatca?ccattttcgc 300

aacaacaaca?accaccattt?tcgcagcaac?aacaaccacc?gttttcacaa?caaccaccaa 360

tttcacagca?gcaacaacca?caatttttgc?agcaacaaca?accaccattt?tcacagcaac 420

agcaaccacc?attttcgcag?caacaacagc?caccatattc?gcagcaacaa?caaccaccat 480

tttcacagca?acaacaacca?ccattttcgc?agcaacaaca?accaccattt?tcgcagcagc 540

aacaacaaca?acaaccacca?tttacacaac?aacaacaacc?accgttttca?caacaaccac 600

caatttcaca?gcagcaacaa?ccaccatttt?cgcagcaaca?acaaccacca?ttttcacagc 660

aacaacaaat?accagttatt?catccatctg?ttctgcagca?gctaaaccca?tgcaaggtat 720

tcctccagca?gcagtgcatc?cctgtggcaa?tgcagcgatg?tcttgctagg?tcacaaatgt 780

tgcagcagag?catttgccat?gtgatgcagc?aacaatgttg?ccagcaattg?cggcaaatcc 840

ccgagcaatc?ccgccatgag?tcaatccgtg?ctatcgtcta?ctctatcatc?ctgcagaagc 900

aacaacaaca?acaacaacaa?caacagggtc?agagtatcat?ccaatatcag?caacaacaac 960

cccaacagtt?gggccaatgt?gtctcccaac?cccaacagca?gttgcagcag?caactcgggc 1020

aacaacctca?acaacaacaa?ttgacacatg?gtgccttttt?gcagccacac?cagatagctc 1080

agcttgaggt?gatgaattcc?attgcactcc?gtaacctgcc?aaggatgtgc?agtgtcaacg 1140

tgccgttgta?cgaaaccacc?actagtgtgc?cattaggcgt?tggcatcgga?gttggtgtct 1200

actgataaga?aaagatctct?agtaatatat?agttggatca?ccgttgttta?gtcgatggat 1260

atgtcgatgc?agcggtgaca?aataatgtgt?cacacaacgt?catgtgtgac?ccgctcaaac 1320

tagttgttta?aattctgaaa?taaaatacaa?ataaagttgt?atcaagacaa?tgttcatatc 1380

ggcattgtgt?ggatatgtcg?atctgattgc?catgcttgca?ggttcataag?tttgtcttgc 1440

ctg 1443

<210>5

<211>1540

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>5

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaagc?aacactagtt 120

aacaccaatc?cacaatgaaa?accttcctcg?tctgtgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aaccaccacc?attttcgcag?caacaacaac 240

caccattttc?acagcaacaa?caaccaccat?tttcgcagca?acaacaatca?ccattttcgc 300

aacaacaaca?acaaccacca?ttttcgcagc?aacaacaacc?accattttca?caacaaccac 360

taatttcaca?gcagcaacaa?ctaccatttt?cacagcaaca?acaaccacaa?ttttcacagc 420

aacagcaacc?accatattcg?cagcaacaac?agccaccata?ttcgcagcaa?caacaaccac 480

cattttcgca?gcaacagcaa?ccaccatttt?cgcagcaaca?acaaccatca?ttttcgcagc 540

aacaacaaca?accaccattt?acacaacaac?aacaaccacc?gttttcacaa?cagtcaccaa 600

tttcacagca?gcagcaacaa?caacaacaac?aacaacaaca?accatttaca?cagcaacagc 660

aaccaccgtt?ttcacaacag?ccaccaattt?cacagcagca?acaaccacca?ttttcgcagc 720

aacaacaacc?accattttca?cagcaacaac?aaataccagt?tattcatcca?tctgttttgc 780

agcagctaaa?cccatgcatg?gtattcctcc?agcagcagtg?catccctgtg?gcaatgcagc 840

gatgtcttgc?taggtcacaa?atgttgcagc?agagcatttg?ccatgtgatg?cagcgacaat 900

gttgccagca?gttgcggcaa?atccccgagc?aatcccgcca?tgagtcaatc?cgtgctatca 960

tctactctat?catcctgcag?cagcagcagc?aacaacaaca?acaacaacaa?caacaacaac 1020

aacagggtca?gagtatcatc?caatatcagc?aacaaccacc?ccaacagttg?ggccaatgtg 1080

tctcccaacc?ccaacagcag?ttgcagcagc?aactcgggca?acaacctcaa?caacaacagt 1140

tggcacatgg?tacctttttg?cagccacacc?agatagctca?acttgaggtg?atgacttcca 1200

ttgcactccg?taccctgcca?agaatgtgca?gtgtcaatgt?gccgttgtac?gaaaccacca 1260

ctagtgtgcc?attaggcgtt?ggcatcggag?ttggtgtcta?ctgataagaa?aagatctcta 1320

gtaatatata?gttggatcac?ggttgtttag?tcgatggata?tgtcgatgta?gcggtgacaa 1380

ataaagtgtc?acacaacgtc?atgtgtgacc?cactcaaaat?agttgtttaa?attctgaaat 1440

aaaatacaaa?taaaggtgta?ttaagaaaat?gttcatatcg?gcattgtgtg?gatgtcgatc 1500

tgattgccat?gcatgcaagt?tcataagttt?gtcttgcctg 1540

<210>6

<211>1441

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>6

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?tacaagcatc?aaagccaagc?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aagcaccgcc?attttcgcag?caacaacaac 240

caccattttc?acagcaacaa?caaccaccat?tttcgcagca?acaacaatca?ccattttcgc 300

aacaacaaca?acaaccacca?tttgcgcagc?aacaacaacc?accgttttca?caacaaccac 360

caatttcaca?gcagcaacaa?ccaccatttt?cacagcaaca?acaaccacaa?ttttcacagc 420

aacaacaacc?accatattcg?cagcaacaac?agccaccata?ttcgcagcaa?caacaaccac 480

cattttcgca?gcaacaacaa?ccaccatttt?cgcagcaaca?acaacaacca?ccatttacac 540

agcagcagca?gcagcaacaa?caacaacaac?catttacaca?gcaacagcaa?ccaccgtttt 600

cacaacagcc?accaatttca?cagcagcaac?aaccaccatt?tttgcagcaa?caacgaccac 660

cattttcacg?gcaacaacaa?ataccagtta?ttcatccatc?tgttttgcaa?cagctaaacc 720

catgcaaggt?attcctccaa?cagcagtgca?tccctgtggc?aatgcagcga?tgtcttgcta 780

ggtcacaaat?gttgcagcag?agcatttgcc?atgtgatgca?gcaacaatgt?tgccagcagt 840

tgcggcaaat?ccccgagcaa?tcccgccatg?agtcaatccg?tgctatcatc?tactctatca 900

tcctgcagca?gcagcagcag?caacaacaac?aacaacaaca?acaacagggt?cagagtatca 960

tccaatatca?gcaacaacaa?ccccaacagt?tgggccaatg?tgtctcccaa?cccctacagc 1020

agttgcagca?gcaactcggg?caacaacctc?aacaacaaca?attggcacac?cagatagctc 1080

agcttgaggt?gatgacttcc?attgcactcc?gtaccctgcc?aacaatgtgc?aatgtcaatg 1140

tgccgttgta?cgaaaccacc?actagtgtgc?cattaggcgt?tggcatcgga?gttggtgtct 1200

actgataaga?aaagatctct?agtaatatat?agttggatca?ccgttgttta?gtcgatggat 1260

atgtcgatgt?agcggtgaca?aataaagtgt?cacacaacgt?catgtgtgac?ccgctcaaac 1320

tagttgttta?aattctgaaa?taaaatacaa?ataaaggtgt?attaagaaaa?tgttcatatc 1380

ggcattgtgt?ggatgtcgat?ctgattgcca?tgcatgcaag?ttcataagtt?tgtcttgcct 1440

g 1441

<210>7

<211>1537

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>7

aaacagaatt?attaaagccg?gtgcaaagaa?ggaaaagagg?tggtgtcccg?gcaactataa 60

ataggcatga?agtataaaga?tcatcacaag?cacaagcatc?aaagccaagc?aacactagtt 120

aacaccaatc?cacaatgaag?accttcctcg?tctttgccct?cctcgctctt?gcggcggcaa 180

gtgccgttgc?gcaaatttca?cagcaacaac?aaccaccacc?attttcgcag?caacaacaac 240

caccattttc?acagcaacaa?caaccaccat?tttcgcagca?acaacaatca?ccattttcgc 300

aacaacaaca?acaaccacca?ttttcgcagc?aacaacaacc?accattttca?caacaaccac 360

taatttcaca?gcagcaacaa?ctaccatttt?cacagcaaca?acaaccacaa?ttttcacagc 420

aacagcagcc?accatattcg?caacaacaac?agccaccata?ttcgcagcaa?caacaaccac 480

cattttcgca?gcaacagcaa?ccaccatttt?cgcagcaaca?acaaccatca?ttttcgcagc 540

aacaacaaca?accaccattt?acacagcaac?aacaaccacc?gttttcacaa?cagtcaccaa 600

tttcacagca?gcagcagcaa?caacaacaac?aacaacaaca?accatttaca?cagcaacagc 660

aaccaccgtt?ttcacaacag?ccaccaattt?cacagcagca?acaaccacca?ttttcgcagc 720

aacaacaacc?accattttca?cagcaacaac?aaataccagt?tattcatcca?tctgttttgc 780

agcagctaaa?cccatgcaag?gtattcctcc?agcagcagtg?catccctgtg?gcaatgcagc 840

gatgtcttgc?taggtcacaa?atgttgcagc?agagcatttg?ccatgtgatg?cagcgacaat 900

gttgccagca?gttgcggcaa?atccccgagc?aatcccgcca?tgagtcaatc?cgtgctatca 960

tctactctat?catcctgcag?cagcagcagc?agcaacaaca?acaacaacaa?caacaacaac 1020

agggtcagag?tatcatccaa?tatcagcaac?aaccacccca?acagttgggc?caatgtgtct 1080

cccaacccca?acagcagttg?cagcagcaac?tcgggcaaca?acctcaacaa?caacagttgg 1140

cacatggtac?ctttttgcag?ccacaccaga?tagctcagct?tgaggtgatg?acttccattg 1200

cactccgtac?cctgccaaga?atgtgcagtg?tcaatgtgcc?gttgtacgaa?accaccacta 1260

gtgtgccatt?aggcgttggc?atcggagttg?gtgtctactg?ataagaaaag?atctctagta 1320

atatttagtt?ggatcaccgt?tgtttagtcg?atggatatgt?cgatgtagcg?gtgacaaata 1380

aagtgtcaca?caacgtcatg?tgtgacccac?tcaaactagt?tgtttaaatt?ctgaaataaa 1440

atacaaataa?aggtgtatta?agaaaatgtt?catatcggca?ttgtgtggat?gtcgatctga 1500

ttgccatgca?tgcaagttca?taagtttgtc?ttgcctg 1537

<210>8

<211>1100

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>8

catctctgat?ccatccaaac?ataattatta?aagttgatgc?aaagaaggaa?agaggtggtg 60

cccggactac?tataaatagg?catgaagtat?aaagatcatc?acaagcacaa?gcatcaaaac 120

caagcaaaac?tagttagcac?caatccatca?tgaagacctt?cctcgtcttt?gccctcctcg 180

ccgttgtggc?aacaagtgcc?attgcgcaga?tggatactag?ctacatccct?ggtttggaga 240

gaccatggca?gcagcaacca?ttaccaccac?aacagacatt?tccacaacaa?ccaccatttt 300

cacaataaca?acaacaacaa?caacaacaac?catttcctca?acaaccatca?ttttcacagc 360

aacaaccacc?attttcacag?caacaaccaa?ttctaccaca?gggaccacca?ttttcacagc 420

aaacacaacc?tgttctaccg?caacaatcac?cattttcaca?gcaacaacaa?ctaattttac 480

ctccacaaca?acaacaacag?cttccgcaac?aacaaatctc?tattgttcaa?ccatccgttt 540

tgcagcagct?aaacccatgc?aaggtattcc?tccagcagca?gtgcagccct?gtggcaatgc 600

cacaacgtct?tgctaggtcg?caaatgtggc?agcagagccg?ttgccatgtg?atgcagcaac 660

aatgttgcca?gcagttgtcg?caaatccccg?aacaatcccg?ctatgatgca?atccgtgcca 720

tcacctactc?catcatccta?caagaacaac?aacagggttt?tgtccaagct?cagcagcaac 780

aaccccaaca?gtcaggtcaa?ggtgtctccc?aatcccaaca?gcagtcgcag?cagcagctcg 840

gacaatgttc?tttccaacaa?cctcaacagc?aactgggtca?acagcctcaa?caacaacagg 900

tacaacaggg?tacctttttg?cagccacacc?agatagctca?ccttgaggtg?atgacttcca 960

ttgcgctccg?taccctgcca?acgatgtgca?gtgtcaatgt?gccattgtac?agctccacca 1020

ctagtgtgcc?attcggagtt?ggcaccggag?ttggtgccta?ctgataagaa?aagatgtcta 1080

gtaatatata?gttggatcac 1100

<210>9

<211>1097

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>9

catctctgat?ccatccaaac?ataattatta?aagttgatgc?aaagaaggaa?agaggtggtg 60

cccggactac?tataaatagg?catgaagtat?aaagatcatc?acaagcacaa?gcatcaaaac 120

caagcaaaac?tagttagcac?caatccatca?tgaagacctt?cctcgtcttt?gccctcctcg 180

ccgttgtggc?aacaagtgcc?attgcgcaga?tggatactag?ctacatccct?ggtttggaga 240

gaccatggca?gcagcaacca?ttaccaccac?aacagacatt?tccacaacaa?ccaccatttt 300

cacaataaca?acaacaacaa?caacaaccat?ttcctcaaca?accatcattt?tcacagcaac 360

aaccaccatt?ttcacagcaa?caaccaattc?taccacaggg?accaccattt?tcacagcaaa 420

cacaacctgt?tctaccgcaa?caatcaccat?tttcacagca?acaacaacta?attttacctc 480

cacaacaaca?acaacagctt?ccgcaacaac?aaatctctat?tgttcaacca?tccgttttgc 540

agcagctaaa?cccatgcaag?gtattcctcc?agcagcagtg?cagccctgtg?gcaatgccac 600

aacgtcttgc?taggtcgcaa?atgtggcagc?agagccgttg?ccatgtgatg?cagcaacaat 660

gttgccagca?gttgtcgcaa?atccccgaac?aatcccgcta?tgatgcaatc?cgtgccatca 720

cctactccat?catcctacaa?gaacaacaac?agggttttgt?ccaagctcag?cagcaacaac 780

cccaacagtc?aggtcaaggt?gtctcccaat?cccaacagca?gtcgcagcag?cagctcggac 840

aatgttcttt?ccaacaacct?caacagcaac?tgggtcaaca?gcctcaacaa?caacaggtac 900

aacagggtac?ctttttgcag?ccacaccaga?tagctcacct?tgaggtgatg?acttccattg 960

cgctccgtac?cctgccaacg?atgtgcagtg?tcaatgtgcc?attgtacagc?tccaccacta 1020

gtgtgccatt?cggagttggc?accggagttg?gtgcctactg?ataagaaaag?atgtctagta 1080

atatatagtt?ggatcac 1097

<210>10

<211>1100

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>10

catctctgat?ccatccaaac?ataattatta?aagttgatgc?aaagaaggaa?agaggtggtg 60

cccggactac?tataaatagg?catgaagtat?aaagatcatc?acaagcacaa?gcatcaaaac 120

caagcaaaac?tagttagcac?caatccatca?tgaagacctt?cctcgtcttt?gccctccttg 180

ccgttgtggc?aacaagtgcc?attgcgcaga?tggatactag?ctgcatccct?ggtttggaga 240

gaccatggca?gcagcaacca?ttaccaccac?aacagacatt?tccacaacaa?ccaccatttt 300

cacaacaaca?acaacaacaa?caacaacaac?catttcctca?acaaccatca?ttttcacagc 360

aacaaccacc?attttcacag?caacaaccaa?ttctaccaca?gggaccacca?ttttcacagc 420

aaacacaacc?tgttctaccg?caacaatcac?cattttcaca?gcaacaacaa?ctaattttac 480

ctccacaaca?acaacaacag?cttccgcaac?aacaaatctc?tattgttcaa?ccatccattt 540

tgcagcagct?aaacccatgc?aaggtattcc?tccagcagca?gtgcagccct?gtggtaatgc 600

cacaacgtct?tgctaggtcg?caaatgtggc?agcagagcag?ttgccatgtg?atgcagcaac 660

aatgttgcca?gcagttgtcg?caaatccccg?aacaatcccg?ctatgatgca?atccgtgcca 720

tcacctaccc?catcatccta?caagaacaac?aacagggttt?tgtccaagct?cagcagcaac 780

aaccccaaca?gtcaggtcaa?ggtgtctccc?aatcccaaca?gcagtcgcag?cagcagctcg 840

gacaatgttc?tttccaacaa?cctcaacagc?aactgggtca?acagcctcaa?caacaacagg 900

tacaacaggg?tacctttttg?cagccacacc?agatagctca?tcttgaggtg?atgacttcca 960

ttgcgctccg?taccctgcca?acgatgtgca?gtgtcaatgt?gccattgtac?agctccacca 1020

ctagtgtgcc?attcggagtt?ggcaccggag?ttggtgccta?ctgataagaa?aagatgtcta 1080

gtaatatata?gttggatcac 1100

<210>11

<211>1106

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>11

catctctgat?ccatccaaac?ataattatta?aagttgatgc?aaagaaggaa?agaggtggtg 60

cccggactac?tataaatagg?catgaagtat?aaagatcatc?acaagcacaa?gcatcaaaac 120

caagcaaaac?tagttagcac?caatccatca?tgaagacctt?cctcgtcttt?gccctcctcg 180

ccattgtggc?aacaagtgcc?attgcgcaga?tggatactag?ctgcatccct?ggtttggaga 240

gacaatggca?gcagcaacca?ttaccaccac?aacagacatt?tccacaacaa?ccaccatttt 300

cacaacaaca?acaacaacaa?caacaacaac?aacaaccatt?tcctcaacaa?acatcatttt 360

cacagcaaca?accaccattt?tcacagcaac?aaccaattct?accacaggga?ccaccatttt 420

cacagcaaac?acaacctgtt?ataccgcaac?aatcaccatt?ttcacagcaa?caacaactaa 480

ttttacctcc?acaacaacaa?caacagcttc?cgcaacaaca?aatctctatt?gttcaaccat 540

ccgttttgca?gcagctaaac?ccatgcaagg?tattcctcca?gcagcagtgc?agccctgtgg 600

caatgccaca?acgtcttgct?aggtcgcaaa?tgtggcagca?gagcagttgc?catgtgatgc 660

agcaacaatg?ttgccagcag?ttgtcgcaaa?tccccgaaca?atcccgctat?gatgcaatcc 720

gtgccatcac?ctactccatc?atcctacaag?aacaacaaca?gggttttgtc?caagctcagc 780

agcaacaacc?ccaacagtca?ggtcaaggtg?tctcccaatc?ccaacagcag?tcgcagcagc 840

agctcggaca?atgttctttc?caacaacctc?aacagcaact?gggtcaacag?cctcaacaac 900

aacaggtaca?acagggtacc?tttttgcagc?cacaccagat?agctcacctt?gaggtgatga 960

cttccattgc?gctccgtacc?ctgccaatga?tgtgcagtgt?caatgtgcca?ttgtacagct 1020

ccaccactag?tgtgccattc?ggagttggca?ccggagttgg?tgcctactga?taagaaaaga 1080

tgtctagtaa?tatatagttg?gatcac 1106

<210>12

<211>1372

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>12

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtgccc 60

gggctactat?aaataggcat?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttgacatcaa?tccacaatga?agacattcct?cgtcttttcc?ctcctcgctc 180

ttgcggcggc?aagtgccgtt?gcgcaaattt?cacagcaaca?acaacaacaa?caaccatttc 240

cgcagcaaca?acaaccacca?ttttcgcagc?aacaagagcc?accatattca?cagcaatagc 300

aaccaccatt?ttcgcaacaa?caacaatcac?cattttcgca?gcaacaacaa?caacaaccac 360

catttttgca?gcaacaccaa?ccaccgtttt?cacaacagcc?accaatttca?cagcagcaac 420

aaccaccatt?ttcacagcaa?caacaaccac?cattttcaca?gcaacaacaa?ccaccatttt 480

cgcagcagca?acaacaacaa?ccaccatttt?cacaacaaca?acaaccaccg?ttttcacaac 540

agccaccaat?ttcacagcaa?caacaaccac?cattttcgca?gcaacaacaa?ccaccatttt 600

cacagcaaca?acaaatacca?gttattcatc?catctgtttt?gcagcaacta?aacccatgca 660

aggtattcct?ccagcagcag?tgcatccctg?tggcaatgca?gcgatgtctt?gctaggtcac 720

aaatgttgca?gcagaggatt?tgccatgtga?tgcagcaaca?atgttgccaa?cagttgcggc 780

aaatccccgg?gcaatcccgc?catgagtcaa?tccgtgctat?catctactct?atcatcctgc 840

agcaacaaca?acaacaacaa?caacaagttc?agagtatcat?ccaagctcag?caacaacaac 900

cccaacagtt?gggccaatgt?gtctcccaac?cccaacaaca?atcgcagcag?caactcgggc 960

aacaacctca?acaacaacaa?ttggcacagg?gtaccttttt?gcagccacac?cagatagctc 1020

aatttgaggt?gatgacttcc?attgcactcc?gtaccttgcc?aacgatgtgc?aatgtcaacg 1080

tgccgttgta?cggaaccacc?actagtgcgc?cattcggcgt?tggcactgga?gttggtgcct 1140

actgataaga?aaagatctct?attaatatat?agttggatca?ccgttgttta?gtcgatggat 1200

atgtcgatgt?agcggtgaca?aataaagtgt?cacacaacac?catgtgtgac?ccgcccaaac 1260

tagttgttta?aattctgaaa?taaaatacaa?ataaagttgt?atcaagacaa?tgttcatatc 1320

agcattgtgc?ggatgtcgat?ctgattgcca?tccttgcaag?ttcataagtt?tg 1372

<210>13

<211>1375

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>13

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtgccc 60

gggctactat?aaataggcct?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttgacaccaa?tccacaatga?agacattcct?cgtcttttcc?ctcctcgctc 180

ttgcggcggc?aagtgccgtt?gcgcaaattt?cacagcaaca?acaacaacaa?caaccatttc 240

cgcagcaaca?acaaccacca?ttttcgcagc?aacaagagcc?accatattca?cagcaatagc 300

aaccaccatt?ttcgcaacaa?caacaatcac?cattttcgca?gcaacaacaa?caacaaccac 360

catttttgca?gcaacaccaa?ccaccgtttt?cacaacagcc?accaatttca?cagcagcaac 420

aaccaccatt?ttcacagcaa?caacaaccac?cattttcaca?gcaacaacaa?ccaccatttt 480

cgcagcagca?acaacaacaa?ccaccatttt?cacaacaaca?acaaccaccg?ttttcacaac 540

agccaccaat?ttcacagcag?caacaaccac?cattttcgca?gcaacaacaa?ccaccatttt 600

cacagcaaca?acaaatacca?gttattcatc?catctgtttt?gcagcaacta?aacccataca 660

aggtattcct?ccagcagcag?tgcatccctg?tggcaatgca?gcgatgtctt?gctaggtcac 720

aaatgttgca?gcagaggatt?tgccatgtga?tgcagcaaca?atgttgccaa?cagttgcggc 780

aaatccccgg?gcaatcccgc?catgagttaa?tccgtgctat?catctactct?atcatcctgc 840

agcaacaaca?acaacaacaa?caacaacaag?tttagagtat?catccaagct?cagcgacaac 900

aaccccaaca?gttgggccaa?tgtgtctccc?aaccccaaca?acaatcgcag?cagcaactcg 960

ggcaacaacc?tcaacaacaa?caattggcac?agggtacctt?tttgcagcca?caccagatag 1020

ctcaatttga?ggtgatgact?tccattgcac?tccgtacctt?gccaacgatg?tgcaatgtca 1080

acgtgctgtt?gtacggaacc?accactagtg?cgccattcgg?cgttggcact?ggagttggtg 1140

cctactgata?agaaaagatc?tctattaata?tatagttgga?tcaccgttgt?ttagtcgatg 1200

gatatgtcga?tgtagcggtg?acaaataaag?tgtcacacaa?caccatgtgt?gacccgccca 1260

aactagttgt?ttaaattctg?aaataaaata?caaataaagt?tgtatcaaga?caatgttcat 1320

atcagcattg?tgcggatgtc?gatctgattg?ccatccttgc?aagttcataa?gtttg 1375

<210>14

<211>1405

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>14

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtggtc 60

gggctactat?aaataggcat?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttaacaccaa?tccacaatga?ggaccttcct?cgtctttgcc?ctcctcgctc 180

ttgcggcggc?aagtgtggtt?gtgcaaattt?cacagcaaca?acaacaacaa?ccatttccgc 240

agcaacaaca?accaccattt?tcgcagcaac?aacagccacc?attttcacaa?caacaacaac 300

caccattttc?gcaacaacaa?caatcaccat?tttcacagca?acaacaacaa?caaccacctt 360

ttttgcagca?acaacaacca?tcattttcac?aacagccacc?aatttcacag?cagcaacaac 420

caccattttc?gcagcaacaa?caaccaccat?tttcacagca?acaacaacca?ccattttcgc 480

agcagcaaca?acaacaacaa?caacaaccac?cattttcaca?acagccacca?atttcacagc 540

agcaacaacc?accatttgtg?cagcaacaac?aaccaccatt?ttcacagcaa?caacaaatac 600

cagttattca?tccatctgtt?ttgcagcaac?taaacccatg?caaggtattc?ctccagcagc 660

agtgcatccc?tgtggcaatg?cagcgatgtc?ttgctaggtc?acaaatgttg?cagcagagga 720

tttgccatgt?gatgcagcaa?caatgttgcc?agcagttgcg?gcaaatcccc?gagcaatccc 780

gccatgagtc?aattcgtgct?atcatctact?ctatcatcct?gcaacaacaa?caacaacaac 840

aacaacaaca?acaacaacaa?caacaagaac?aacaacaaca?acaacaacaa?catcaacaag 900

ttcagagtat?catccaagct?cagcaacaac?aaccccaaca?gttgggccta?tgtgtctccc 960

aaccccaaca?acagtcgcag?cagcaactcg?ggcaacaacc?tcaacaacaa?caattggcac 1020

agggtacctt?tttgcagcca?cactagatag?ctcagtttga?ggtgatgact?tccattgcgc 1080

tccgtacctt?gccaacgatg?tgcaatgtca?acgtgccgtt?gtacggaacc?accactagtg 1140

cgccattcgg?tgttggcgct?ggagttggtg?cctactgata?agaaaagatc?tcttgtaata 1200

tatagttgga?tcaccgttgt?ttagtcgatg?gatatgtcga?tgtagttgtg?acaaataaag 1260

tgtcacacaa?caccatgtgt?gacccagcca?aactagttgt?ttaaattctg?aaataaaata 1320

caaataaagt?tgtatcaaga?caatgttcat?atcagtattg?tgcggatgtc?gatctgattg 1380

ccatccttgc?aagttcataa?gtttg 1405

<210>15

<211>1381

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>15

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtgccc 60

gggctactat?aaataggcat?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttgacaccaa?tccacaatga?agacattcct?tgtctttgcc?ctcctcgctc 180

ttgcggcggc?aagtgccgtt?gcgcaaattt?cacagcaaca?acaacaacaa?ccatttccgc 240

ggcaacaaca?accaccattt?tcgcagcaac?aagagccacc?atattcacag?caacaacaac 300

caccattttt?gcaacaacaa?caatcaccat?tttagcagca?acaacaacaa?caaccaccat 360

ttttgcagca?acaccaacca?ccgttttcac?aacagccacc?aatttcacag?cagcaacaac 420

caccattttc?gcagcaacaa?caaccaccat?tttcacagca?acaacaacca?ccattttcgc 480

agcagcagca?acaacaacaa?ccaccatttt?cacagaaaca?acaaccatcg?ttttcacaac 540

agccaccaat?ttcacagcag?caacaaccac?cattttcgca?gcaacaacaa?ccaccatttt 600

cacagcaaca?acaaatacca?gttattcatc?catctgtttt?gcagcaacta?aacccatgca 660

aggtattcct?ccagcagcag?tgcatccctg?tggcaatgca?gcgatgtctt?gctaggtcgc 720

aaatgttgca?gcaaaggatt?tgccatgtga?tgcagcaaca?atgttgccaa?cagttgcggc 780

aaatccccgg?gcaatcccgc?catgagtcaa?tccgtgctat?catctactct?atcatcctgc 840

aacaacaaca?acaacaacaa?caacaacaac?aacaagttca?gagtatcatc?caagctcagc 900

aacaacaacc?ccaacaattg?ggccaatgtg?tcttccaacc?ccaacaacaa?tcgcagcagc 960

aactcgggca?acaacctcaa?caacaacaat?tggcacaggg?tacctttttg?cagccacacc 1020

agatagctca?gtttgaggtg?atgacttcca?ttgcactccg?taccttgcca?acgatgtgca 1080

atgtcaacgt?gccgttgtac?ggaaccacca?ctagtgcgcc?attcggcgtt?ggcactggag 1140

ttggtgccta?ctgataagaa?aagatctcta?gtaatatata?gttggatcac?tgttgtttag 1200

tcgatggata?tgtcgatgta?gcggtgacaa?ataaagtgtc?acacaacacc?atgtgtgacc 1260

cgcccaaact?agttgtttaa?attctgaaat?aaaatacaaa?taaagttgta?tcaagacaat 1320

gttcatatca?gcattgtgcg?gatgtcgatc?tgattgccat?ccttgcaagt?tcataagttt 1380

g 1381

<210>16

<211>1381

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>16

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtgccc 60

gggctactat?aaataggact?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttgacaccaa?tccacaatga?agacattcct?cgtcttttcc?ctcctcgctc 180

ttgcggcggc?aagtgccgtt?gcgcaaattt?cacagcaaca?acaacaacaa?caaccatttc 240

cgcagcaaca?acaaccacca?ttttcgcagc?aacaagagcc?accatattca?cagcaatagc 300

aaccaccatt?ttcgcaacaa?caacaatcac?cattttcgca?gcaacaacaa?caacaaccac 360

catttttgca?gcaacaccaa?ccaccgtttt?cacaacagcc?accaatttca?cagcagcaac 420

aaccaccatt?ttcacagcaa?caacaaccac?cattttcaca?gcaacaacaa?ccaccatttt 480

cgcagcagca?acaacaacaa?ccaccatttt?cacaacaaca?acaaccaccg?ttttcacaac 540

agccaccaat?ttcacagcag?caacaaccac?cattttcgca?gcaacaacaa?ccaccatttt 600

cacagcaaca?acaaatacca?gttattcatc?catctgtttt?gcagcaacta?aacccatgca 660

aggtattcct?ccagcagcag?tgcatccctg?tggcaatgca?gcgatgtctt?gctaggtcac 720

aaatgttgca?gcagaggatt?tgccatgtga?tgcagcaaca?atgttgccaa?cagttgcggc 780

aaatccccgg?gcaatcccgc?catgagttaa?tccgtgctat?catctactct?atcatcctgc 840

agcaacaaca?acaacaacaa?caacaacaac?aacaagttca?gagtatcatc?caagctcagc 900

gacaacaacc?ccaacagttg?ggccaatgtg?tctcccaacc?ccaacaacaa?tcgcagcagc 960

aactcgggca?acaacctcaa?caacaacaat?tggcacaggg?tacctttttg?cagccacacc 1020

agatagctca?atttgaggtg?atgacttcca?ttgcactccg?taccttgcca?gcgatgtgca 1080

atgtcaacgt?gctgttgtac?ggaaccacca?ctagtgcgcc?attcggcgtt?ggcactggag 1140

ttggtgccta?ctgataagaa?aagatctcta?ttaatatata?gttggatcac?cgttgtttag 1200

tcgatggata?tgtcgatgta?gcggtgacaa?ataaagtgtc?acacaacacc?atgtgtgacc 1260

cgcccaaact?agttgtttaa?attctgaaat?aaaatacaaa?taaagttgta?tcaagacaat 1320

gttcatatca?gcattgtgcg?gatgtcgatc?tgattgccat?ccttgcaagt?tcataagttt 1380

g 1381

<210>17

<211>1387

<212>DNA

＜213〉Triticum common wheat (Triticum aestivum L.)

<400>17

ttcagatgca?gccaaacaaa?tttattaaag?ctgatgcaaa?gaagaaaaga?ggtggtgccc 60

gggctactat?aaataggcat?gaagtataaa?gatcatcaca?agcacaagca?tcaaaaccaa 120

gcaacactag?ttgacaccaa?tccacaatga?agacattcct?tgtctttgcc?ctcctcgctc 180

ttgcggcagc?aagtgccgtt?gcgcaaattt?cacagcaaca?acaacaacaa?ccatttccgc 240

ggcaacaaca?accaccattt?ttgcagcaac?aagagccacc?atattcacag?caacagcaac 300

caccattttc?gcaacaacaa?caatcaccat?tttagcagca?acaacaacaa?caaccaccat 360

ttttgcagca?acaccaacca?ccgttttcac?aacagccacc?aatttcacag?cagcaacaac 420

caccattttc?gcagcaacaa?caaccaccat?tttcacagca?acaacaacca?ccattttcgc 480

agcagcaaca?acaacaacaa?ccaccatttt?cacagaaaca?acaaccatcg?ttttcacaac 540

agccaccaat?ttcacagcag?caacaaccac?cattttcgca?gcaacaacaa?ccaccatttt 600

cacagcaaca?acaaatacca?gttattcatc?catctgtttt?gcagcaacta?aacccatgca 660

aggtattcct?ccagcagcag?tgcatccctg?tggcaatgca?gcgatgtctt?gctaggtcgc 720

aaatgttgta?gcaaaggatt?tgccatgtga?tgcagcaaca?atgttgccaa?cagttgcggc 780

aaatccccgg?gcaatcccgc?catgagtcaa?tccgtgctat?catctactct?atcatcctgc 840

aacaacaaca?acaacaacaa?caacaacaac?aacaacaaca?agttcagagt?atcatccaag 900

ctcagcaaca?acaaccccaa?caattgggcc?aatgtgtctt?ccaaccccaa?caacaatcgc 960

agcagcaact?cgggcaacaa?cctcaacaac?aacaattggc?acagggtacc?tttttgcagc 1020

cacaccagat?agctcagttt?gaggtgatga?cttccattgc?actccgtacc?ttgccaacga 1080

tgtgcaatgt?caacgtgccg?ttgtacggaa?ccaccactag?tgcgccattc?ggcgttggca 1140

ctggagttgg?tgcctactga?taagaaaaga?tctctagtaa?tatatagttg?gatcactgtt 1200

gtttagtcga?tggatatgtc?gatgtagcgg?tgacaaataa?agtgtcacac?aacaccatgt 1260

gtgacccgcc?caaactagtt?gtttaaattc?tgaaataaaa?tacaaataaa?gttgtatcaa 1320

gacaatgttc?atatcagcat?tgtgcggatg?tcgatctgat?tgccatcctt?gcaagttcat 1380

aagtttg 1387

<210>18

<211>21

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>18

aaacagaatt?attaaagccg?g 21

<210>19

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>19

ttcagatgca?gccaaacaa 19

<210>20

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>20

ggttgttgtt?gttgcagca 19

<210>21

<211>22

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>21

gctgtgcttg?gatgatactc?ta 22

<210>22

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>22

gtggctgttg?tgaaaacga 19

<210>23

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>23

tggggttggg?agacacata 19

<210>24

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>24

ggcacagacg?aggaaggtt 19

<210>25

<211>19

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>25

gctgctgctg?ctgtgtaaa 19

<210>26

<211>21

<212>DNA

＜213〉artificial sequence

<220>

<223>

<400>26

aaacaacggt gatccaacta?a 21

Claims (7)

1, differentiate that wheat to be measured is the primer special that carries the wheat breed of which kind of Glu-A3 protein protomer, comprise in following seven pairs of primers at least one pair of:

(1) primer of the composition of Nucleotide shown in the sequence 20 of Nucleotide and sequence table shown in the sequence 18 of sequence table is right;

(2) primer of the composition of Nucleotide shown in the sequence 21 of Nucleotide and sequence table shown in the sequence 19 of sequence table is right;

(3) primer of the composition of Nucleotide shown in the sequence 22 of Nucleotide and sequence table shown in the sequence 18 of sequence table is right;

(4) primer of the composition of Nucleotide shown in the sequence 23 of Nucleotide and sequence table shown in the sequence 19 of sequence table is right;

(5) primer of the composition of Nucleotide shown in the sequence 24 of Nucleotide and sequence table shown in the sequence 18 of sequence table is right;

(6) primer of the composition of Nucleotide shown in the sequence 25 of Nucleotide and sequence table shown in the sequence 18 of sequence table is right;

(7) primer of the composition of Nucleotide shown in the sequence 26 of Nucleotide and sequence table shown in the sequence 18 of sequence table is right.

2, primer special as claimed in claim 1 is characterized in that: described primer special is made up of described seven pairs of primers.

3, a kind ofly differentiate that wheat to be measured is the test kit that carries the wheat breed of which kind of Glu-A3 protein protomer, comprise claim 1 or 2 described primer specials.

4, claim 1 or 2 described primer specials differentiate that in preparation wheat to be measured is the application of carrying in the test kit of wheat breed of which kind of Glu-A3 protein protomer.

5, a kind ofly differentiate that wheat to be measured is the method for carrying the wheat breed of which kind of Glu-A3 protein protomer, comprises the steps:

1) genomic dna with wheat to be measured is a template, carries out pcr amplification with claim 1 or 2 described primer specials;

2) detect pcr amplification product, judge that by the size of pcr amplification product wheat to be measured is the wheat breed that carries which kind of Glu-A3 protein protomer.

6, claim 1 or 2 described primer specials, the described test kit of claim 3 or the application of the described method of claim 5 in wheat breeding.

7, application as claimed in claim 6 is characterized in that: described wheat is a common wheat.

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