CN101565901A - Wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment - Google Patents
Wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment Download PDFInfo
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- CN101565901A CN101565901A CNA2009100315513A CN200910031551A CN101565901A CN 101565901 A CN101565901 A CN 101565901A CN A2009100315513 A CNA2009100315513 A CN A2009100315513A CN 200910031551 A CN200910031551 A CN 200910031551A CN 101565901 A CN101565901 A CN 101565901A
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Abstract
The invention provides a wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment, which belongs to the technical field of application of dyeing and finishing of wool fabric in the wool-spinning industry. The method aims to overcome the defects that the chlorination-process for wool-felting prevention seriously pollutes the environment and the single protease-method treatment is poor in fabric wettability and high in felting rate in order to achieve the aim of optimizing the protease treatment effect of the wool fabric. After all-wool grey fabric is extracted to remove free oily impurities, the all-wool grey fabric is sequentially pretreated with cutinase and keratinase solutions so as to improve the surface hydrophilicity of fiber scales and the accessibility of protease molecules, and then the wool fabric is subjected to reduction processing in a protease solution, thus biological processing combined with wool enzyme reduction is realized. The method makes the prior chlorination-process felting prevention replaced with a biological-method wool-felting prevention process, and the method not only effectively improve the reduction rate and anti-felting effect of the wool fabric, and promote the improvement of fabric wettability, but also can effectively increase the dyeing depth of fabric, and reduce fiber damage in reduction processing, and is favorable for protecting ecological environment.
Description
Technical field
A kind of Wool-fabric biological anti-felting method based at, keratinase and Protease Treatment belongs to the applied technical field that woolen dyeing and finishing is processed in the wool industry.
Background technology
Wool has feltability, and wool fabric is subjected to external force and does the time spent and can produce felting under humid tropical condition, area is shunk, alteration of form, and fine hair is outstanding, and elasticity reduces, and feel is coarse, appearance of fabrics and take performance and had a strong impact on.Therefore, need carry out to remove scale layer to wool fabric be the decrement feld proofing of purpose.At present, the wool anti-felting finishing effect better and most widely used be chloridizing, handle as adopting hypochlorite or DCCA salt.Fiber was easily inhaled the chlorine yellowing when chloridizing was handled wool, and fabric feeling is coarse, and is easily irregular during dyeing, and the organic chloride content in the processed waste water is quite high, and environment is had adverse effect.
The wool protease anti-felting is to utilize protease the hydrolysis of the big molecule peptide bond of wool fibre to be made the generation such as scale, cell membrane complexes of wool be partly dissolved, reach part and remove the purpose of scale, have characteristics such as treatment conditions gentleness, energy savings and minimizing pollution.Because the scale top layer of wool is made up of the lipid material of marshalling, and combine with ester bond and thioester bond, have extremely strong hydrophobicity with protein under the lipoid layer.Therefore, when wool being carried out feld proofing, generally need carry out Chemical Pretreatment, increasing the hydrophily of fiber surface, and then handle, remove wool surface scale with protease to the wool surface with protease.Though the conventional chemical preliminary treatment can increase the decrement effect of protease, but because the fiber scale layer processing uniformity is wayward, easily cause the wool fibre internal injury, show as that fabric weightless increases, powerful decline and felting is effect improved less, therefore in wool enzyme decrement felt proofing technology, be difficult to obtain practical application.
At is mainly derived from fungi and bacterium, be a kind ofly can destroy the ester bond of cutin polymer molecule and make its hydrolase that is hydrolyzed to monomer and micromolecule oligomer, the ester bond in the hydrophobicity lipoid structure of wool scale top layer is had certain catalyzing hydrolysis ability.Keratinase is a kind of keratic reduction enzyme of can specificity degrading, by multiple microorganisms such as fungi, actinomyces and bacteriums, disulfide bond becomes cysteine compound in the energy hydrolysis wool scale layer, to the fine and close generation of wool scale layer " loosening " effect, be beneficial to follow-up keratic further hydrolysis.In the preventing felting and shrinking of wood fabric processing that this law is addressed, wool fabric is after at is handled, and the wettability of fiber surface makes moderate progress along with the removal of lipides, and the accessibility of enzyme molecule improves in the following process; Keratinase has further increased protease molecule to the keratic hydrolysis efficiency of wool fibre scale to the reduction of the disulfide bond in the fiber scale layer, and the effect of woolen enzyme process felt proofing improves.Action principle signal reaction equation:
Summary of the invention
Purpose of the present invention is intended to improve the processing effect of protease to preventing felting and shrinking of wood fabric.Use the present invention can improve the wettability of wool fabric and the effect of biological anti-felting, it is big to solve the fibre damage of traditional chloridizing preventing felting and shrinking of wood fabric, and the technical problem that environmental pollution is serious reaches the optimization process effect, improves environment, improves the purpose of all woolen product quality.
Technical scheme of the present invention: the Wool-fabric biological anti-felting method of a kind of at, keratinase and Protease Treatment, all-wool grey fabric behind the abstraction impurity removal is used at, keratinase solution-treated successively, use again protease carry out wool fabric protease post processing processing;
The extraction preliminary treatment: wool fabric places chloroform-methanol or carbon tetrachloride-methanol solvate, reflux extraction 2-24 hour, more successively with ethanol or methyl alcohol, and hot water wash remove the cloth cover residual impurity;
At is handled: the wool fabric after the extraction is impregnation process in the solution of mass concentration 0.5%～5.0% at, wetting and penetrating agent 0～20g/L, 30～60 ℃ of temperature, and pH 7.0～9.0, and 1～6 hour time, it is fully clean to handle the back fabric;
Keratinase is handled: the wool fabric impregnation process in the solution of mass concentration 1%～5.0% keratinase, wetting and penetrating agent 0～20g/L after at is handled, and 40～60 ℃ of temperature, pH 8.0～9.0, and 1～8 hour time, it is fully clean to handle the back fabric;
Protease Treatment: the wool fabric impregnation process in the solution of mass concentration 0.2%～2.0% protease, wetting and penetrating agent 0～20g/L after keratinase is handled, 30 ℃～60 ℃ of temperature, pH 7.0～9.0,0.5～2 hour time;
In at, keratinase and Protease Treatment, adopt adding polyoxyethylene-type non-ionic surface active agent or anion surfactant to make wetting and penetrating agent; Or do not add any wetting and penetrating agent;
Enzyme goes out: add 0.5～5.0mol/L trichloroacetic acid and make the protease inactivation in Protease Treatment liquid, or handle certain hour more than the rising enzyme liquid temp to 90 ℃;
Washing: fabric is fully washed with hot water and cold water respectively behind the enzyme that goes out.
Described Wool-fabric biological anti-felting method is applicable to that the wool fabric of processing comprises all-wool gabardine, serge, valetin worsted fabric and drap-de-berry kind.
The enzyme of using is selected different types of at, keratinase and protease for use.
Beneficial effect of the present invention: the Wool-fabric biological anti-felting method of a kind of at of the present invention, keratinase and Protease Treatment is non-single protease method, also be different from existing oxidation processes and protease process combined, but adopted at and keratinase preliminary treatment combination, carry out the three-steps process that the protease decrement combines again.With only compare through the sample of oxidation pre-treatment and Protease Treatment, the wool fabric of this law processing has characteristics preferably, under the situation that does not add any surfactant, the wetting time of wool fabric obviously shortens after the enzyme decrement, contact angle reduces.Under the close reduction rate condition, fibre damage is lower than hydrogen peroxide and protease group technology treatment samples, and the felting rate is lower, and dyeability is improved.
The specific embodiment
Embodiment 1
Fabric variety: all-wool gabardine.
(1) extraction preliminary treatment: wool fabric places chloroform: (87: 13, v/v) in the organic solvent, reflux extraction was 4 hours under 55 ℃ of conditions, removes the cloth cover residual impurity with ethanol and hot water wash respectively again for methyl alcohol.
(2) oxidation processes: handle and bathe: 30% hydrogen peroxide 20g/L, the solution of wetting and penetrating agent 0g/L, 50 ℃ of temperature, pH 8.0,0.5 hour processing time.
(3) at is handled: handle and bathe: at 5.0%owf, and the solution of wetting and penetrating agent 0g/L, 60 ℃ of temperature, pH 8.0,4 hours processing times.
(4) keratinase is handled: handle and bathe: at 5.0%owf, and the solution of wetting and penetrating agent 0g/L, 55 ℃ of temperature, pH 8.5,8 hours time.
(5) Protease Treatment: handle and bathe: protease 2%owf, the solution of wetting and penetrating agent 0g/L, 55 ℃, pH8.5,1 hour processing time.
Sample 1: only through peroxidating and Protease Treatment (step 1,2 and 5);
Sample 2: handle (step 1,3,4 and 5) through at, keratinase preliminary treatment and three steps of protease.
After above-mentioned PROCESS FOR TREATMENT, the reduction rate of sample 1 is 4.9%, and wetting time is 11min, and the felting rate is 4.9%; The reduction rate of sample 2 is 5.4%, and wetting time is 1.2min, and the felting rate is 3.1%.
Embodiment 2
Fabric variety: all woolen woman clothing.
(1) extraction preliminary treatment: wool fabric places carbon tetrachloride: (87: 13, v/v) in the organic solvent solution, reflux extraction was 4 hours under 55 ℃ of conditions, removes the cloth cover residual impurity with ethanol and hot water wash respectively again for methyl alcohol.
(2) at is handled: handle and bathe: the solution of at 2.0%owf, wetting and penetrating agent 1g/L, and 60 ℃ of temperature, pH 8.0,4 hours processing times.
(3) keratinase is handled: handle and bathe: the solution of at 5.0%owf, wetting and penetrating agent 0g/L, and 55 ℃ of temperature, pH 8.5,8 hours time.
(4) Protease Treatment: handle and bathe: the solution of protease 1%owf, 55 ℃, pH 8.5,1 hour processing time.
Sample 1: bathe processing (step 1,2 and 4) through at, protease two;
Sample 2: handle (step 1,2,3 and 4) through at, keratinase preliminary treatment and three steps of protease.
After above-mentioned PROCESS FOR TREATMENT, the reduction rate of sample 1 is 4.2%, and wetting time is 5.5min, and the felting rate is 5.1%; The reduction rate of sample 2 is 4.7%, and wetting time is 1.2min, and the felting rate is 2.9%.
Claims (3)
1. the Wool-fabric biological anti-felting method of an at, keratinase and Protease Treatment, it is characterized in that the all-wool grey fabric behind the abstraction impurity removal is used at, keratinase solution-treated successively, use again protease carry out wool fabric protease post processing processing;
The extraction preliminary treatment: wool fabric places chloroform-methanol or carbon tetrachloride-methanol solvate, reflux extraction 2-24 hour, more successively with ethanol or methyl alcohol, and hot water wash remove the cloth cover residual impurity;
At is handled: the wool fabric after the extraction is impregnation process in the solution of mass concentration 0.5%～5.0% at, wetting and penetrating agent 0～20g/L, 30～60 ℃ of temperature, and pH7.0～9.0,1～6 hour time, it is fully clean to handle the back fabric;
Keratinase is handled: the wool fabric impregnation process in the solution of mass concentration 1%～5.0% keratinase, wetting and penetrating agent 0～20g/L after at is handled, and 40～60 ℃ of temperature, pH8.0～9.0,1～8 hour time, it is fully clean to handle the back fabric;
Protease Treatment: the wool fabric impregnation process in the solution of mass concentration 0.2%～2.0% protease, wetting and penetrating agent 0～20g/L after keratinase is handled, 30 ℃～60 ℃ of temperature, pH7.0～9.0,0.5～2 hour time;
In at, keratinase and Protease Treatment, adopt adding polyoxyethylene-type non-ionic surface active agent or anion surfactant to make wetting and penetrating agent; Or do not add any wetting and penetrating agent;
Enzyme goes out: add 0.5～5.0mol/L trichloroacetic acid and make the protease inactivation in Protease Treatment liquid, or handle certain hour more than the rising enzyme liquid temp to 90 ℃;
Washing: fabric is fully washed with hot water and cold water respectively behind the enzyme that goes out.
2. the Wool-fabric biological anti-felting method of a kind of at according to claim 1, keratinase and Protease Treatment is characterized in that the wool fabric that is applicable to processing comprises all-wool gabardine, serge, valetin worsted fabric and drap-de-berry kind.
3. the Wool-fabric biological anti-felting method of a kind of at according to claim 1, keratinase and Protease Treatment is characterized in that the enzyme of using selects different types of at, keratinase and protease for use.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100315513A CN101565901B (en) | 2009-04-22 | 2009-04-22 | Wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment |
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| CN2009100315513A CN101565901B (en) | 2009-04-22 | 2009-04-22 | Wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment |
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| CN101565901B CN101565901B (en) | 2010-12-01 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102102293A (en) * | 2010-12-23 | 2011-06-22 | 北京中纺化工股份有限公司 | Shrink-resistance machine-washable finishing method of slenderized wool |
| CN102965956A (en) * | 2012-10-19 | 2013-03-13 | 江南大学 | Wool worsted fabric compound enzyme padding-steaming stacking anti-felting finishing technology |
| CN102965958A (en) * | 2012-10-31 | 2013-03-13 | 江南大学 | Wool product two-bath process anti-felting treatment technology based on composite bio-enzyme |
| CN102965955A (en) * | 2012-10-19 | 2013-03-13 | 江南大学 | Cutinase, keratinase and protease one-bath process anti-felting technology |
| CN102978956A (en) * | 2012-10-31 | 2013-03-20 | 江南大学 | Wool fabric complex phosphoesterasum padding-room temperature rolling yarding anti-felting tidying craft |
| CN104256931A (en) * | 2014-08-26 | 2015-01-07 | 湖州圣绒服饰有限公司 | Production method of environment-friendly cashmere garment |
| CN105986484A (en) * | 2015-02-13 | 2016-10-05 | 天津滨海东方科技有限公司 | Continuous processing technology for non-shrink treatment of chlorine-free wool top and wool product |
| CN107083685A (en) * | 2017-05-16 | 2017-08-22 | 无锡协新毛纺织股份有限公司 | A kind of method arranged using keratinase and chitosan to wool fabric |
| CN107177986A (en) * | 2017-05-24 | 2017-09-19 | 无锡协新毛纺织股份有限公司 | A kind of method for carrying out feld proofing to wool fabric using modified protein enzyme |
| CN110499651A (en) * | 2019-09-16 | 2019-11-26 | 江苏阳光股份有限公司 | The processing method of worm's ovum shell in a kind of fleece fabrics |
| CN117286126A (en) * | 2023-09-27 | 2023-12-26 | 北京华腾信和科贸有限公司 | Long-time heat-resistant acid-resistant keratinase and application thereof in wool sample treatment |
-
2009
- 2009-04-22 CN CN2009100315513A patent/CN101565901B/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102102293A (en) * | 2010-12-23 | 2011-06-22 | 北京中纺化工股份有限公司 | Shrink-resistance machine-washable finishing method of slenderized wool |
| CN102965956A (en) * | 2012-10-19 | 2013-03-13 | 江南大学 | Wool worsted fabric compound enzyme padding-steaming stacking anti-felting finishing technology |
| CN102965955A (en) * | 2012-10-19 | 2013-03-13 | 江南大学 | Cutinase, keratinase and protease one-bath process anti-felting technology |
| CN102965958A (en) * | 2012-10-31 | 2013-03-13 | 江南大学 | Wool product two-bath process anti-felting treatment technology based on composite bio-enzyme |
| CN102978956A (en) * | 2012-10-31 | 2013-03-20 | 江南大学 | Wool fabric complex phosphoesterasum padding-room temperature rolling yarding anti-felting tidying craft |
| CN104256931B (en) * | 2014-08-26 | 2016-02-10 | 湖州圣绒服饰有限公司 | A kind of preparation method of environmental protection goat fleecy sweatshirt |
| CN104256931A (en) * | 2014-08-26 | 2015-01-07 | 湖州圣绒服饰有限公司 | Production method of environment-friendly cashmere garment |
| CN105986484A (en) * | 2015-02-13 | 2016-10-05 | 天津滨海东方科技有限公司 | Continuous processing technology for non-shrink treatment of chlorine-free wool top and wool product |
| CN105986484B (en) * | 2015-02-13 | 2018-05-29 | 天津滨海东方科技有限公司 | Continuous processing technology without chlorine wool tops and wool product anti-shrinking |
| CN107083685A (en) * | 2017-05-16 | 2017-08-22 | 无锡协新毛纺织股份有限公司 | A kind of method arranged using keratinase and chitosan to wool fabric |
| CN107177986A (en) * | 2017-05-24 | 2017-09-19 | 无锡协新毛纺织股份有限公司 | A kind of method for carrying out feld proofing to wool fabric using modified protein enzyme |
| CN107177986B (en) * | 2017-05-24 | 2019-11-08 | 无锡协新毛纺织股份有限公司 | A method of feld proofing is carried out using modification proteases on wool fabric |
| CN110499651A (en) * | 2019-09-16 | 2019-11-26 | 江苏阳光股份有限公司 | The processing method of worm's ovum shell in a kind of fleece fabrics |
| CN117286126A (en) * | 2023-09-27 | 2023-12-26 | 北京华腾信和科贸有限公司 | Long-time heat-resistant acid-resistant keratinase and application thereof in wool sample treatment |
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| CN101565901B (en) | 2010-12-01 |
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Assignee: Jiangsu Sunshine Co., Ltd. Assignor: Jiangnan University Contract record no.: 2011320001077 Denomination of invention: Wool-fabric biological anti-felting method based on cutinase, keratinase and protease treatment Granted publication date: 20101201 License type: Exclusive License Open date: 20091028 Record date: 20110801 |
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