CN101565683A - Bacillus polymyxa and method for preparing optically-pure R, R type 2, 3-butanedio by utilizing same - Google Patents
Bacillus polymyxa and method for preparing optically-pure R, R type 2, 3-butanedio by utilizing same Download PDFInfo
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- CN101565683A CN101565683A CNA2009100268071A CN200910026807A CN101565683A CN 101565683 A CN101565683 A CN 101565683A CN A2009100268071 A CNA2009100268071 A CN A2009100268071A CN 200910026807 A CN200910026807 A CN 200910026807A CN 101565683 A CN101565683 A CN 101565683A
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Abstract
The invention discloses bacillus polymyxa, which is classified and denominated as bacillus polymyxa ZJ-9, wherein a preserving number of the bacillus polymyxa is CGMCC NO.3044. The invention also discloses a method for preparing optically-pure R, R type 2, 3-butanedio by utilizing the bacillus polymyxa, which comprises the following steps that: the strain with CGMCC NO.3044 as the preserving number is inoculated into a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water for culture, and the optically-pure R, R type 2, 3-butanedio is produced through the fermentation, wherein the carbon source is jerusalem artichoke inulin which is not subjected to hydrolysis treatment. The optical purity of the optically-pure R, R type 2, 3-butanedio generated by the method can reach more than 98 percent. The stain can directly utilize the jerusalem artichoke inulin, has the advantages of extensive culture conditions and convenient and simple operation; and the optically-pure R, R type 2, 3-butanedio has large yield, high optical purity and low production cost, and is favorable for industrialized mass production.
Description
Technical field
The invention belongs to the fermentation engineering field, relate to a kind of Paenibacillus polymyxa and utilize its preparation optical purity R, R type 2, the method for 3-butyleneglycol.
Background technology
Energy-source plant---jerusalem artichoke is a kind of per nnial herb, is commonly called as Jerusalem artichoke, Jerusalem artichoke, has advantages such as salt tolerant alkali, output is big.Containing inulin (claiming synanthrin again) in the jerusalem artichoke, is polyfructosan, can account for weight in wet base 15～20%, dry weight about 80%, beach wasteland biomass can reach 3～6 tons/mu.Utilize the large-scale planting of wasteland and beach resource area development jerusalem artichoke, and with serve as the industrial chain that the basis forms the bio-based product development, can make full use of and improve the beach saline-alkali ground of the length and breadth of land, added value of raising jerusalem artichoke and coastal peasant's plantation enthusiasm.When obtaining the industrial economy benefit, can also realize ecological management, solve resource and problem of environment, have a high potential.Utilize the jerusalem artichoke inulin hydrolysis to produce fructose or oligofructose, caused the extensive concern of Chinese scholars in recent years, become the research focus.
2, the 3-butyleneglycol is four important carbon hardware and software platform compounds, and is of many uses, may be used on a plurality of fields such as chemical industry, food, fuel and aerospace, has biological degradability.2, there are 3 optically active isomers in the 3-butyleneglycol, is respectively R, R type, S, and S type and meso type (meso-form), structural formula is as follows:
Chirality R, R-2, the 3-butyleneglycol can be used for antifreezing agent etc., and in addition, substrate inevitable requirements such as synthesizing chiral compound such as medicine have 2 of single configuration, the 3-butyleneglycol.
At present chemical method produces 2, and the 3-butyleneglycol mainly is that the four carbon hydrocarbons hydrolysis under high temperature, high pressure that produces during with petroleum cracking obtains, and product is 2 of mixed type, the 3-butyleneglycol.Biological process produces 2, the main bacteria seed of 3-butyleneglycol has: Klebsiella (Klebsiella), bacillus (paenibacillus), Rhodopseudomonas (Pseudomonas) and serratia (Serratia), in these bacterial classifications, the Paenibacillus polymyxa (paenibacillus polymyxa) of acid-producing Klebsiella bacterium of Klebsiella (Klebsiella oxytoca) and bacillus demonstrates higher production 2, the potentiality of 3-butyleneglycol.Mentioned microorganism fermentation produces 2, the research of 3-butyleneglycol all with monose as raw material, as glucose, wood sugar etc., do not find as yet with without the polysaccharide of any hydrolysis treatment directly as the way of substrate, the fermentation of saccharification limit, limit.In addition, microorganism synthetic 2,3-butyleneglycol great majority are mixtures of optically active isomer.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Paenibacillus polymyxa that can produce inulinase, is used to prepare optical purity R, R type 2,3-butyleneglycol.
The technical problem that the present invention also will solve provides above-mentioned Paenibacillus polymyxa utilization and prepares optical purity R as sole carbon source, R type 2, the method for 3-butyleneglycol without the jerusalem artichoke inulin of any hydrolysis treatment.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
The microorganism strains of seed selection of inventor laboratory and preservation is Paenibacillus polymyxa (paenibacilluspolymyxa) ZJ-9, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) at present, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, the numbering of registering on the books is CGMCC No.3044, and preservation date is: on April 29th, 2009.With this bacterium as producing bacterial strain.
CGMCC No.3044 bacterial strain has following character:
1, colonial morphology feature:
On nutrient agar medium, 30 ℃ cultivate 48h occur white small colonies (
=0.6～1.2mm), bacterium colony is rounded, smooth surface, and sticking shape, center projections, transparent.Proper extension is cultivated, and bacterium colony increases, and the size and dimension of cell changes very little.
2, physiology and biochemical characteristic:
A. culture temperature: 28～39 ℃, optimum temperuture is 30 ℃;
B. in pH 5.0～7.5 scopes, grow;
C. pigment production: do not produce pigment;
D. anti-NaCl concentration: can grow in 5% concentration.
3,16S rDNA sequential analysis:
Record the most of sequence 1498bp of 16S rDNA, specifically shown in SEQ ID No.1.The relevant kind of check order row from the GeneBank database compared, make up the phylogenetic tree that 16S rDNA total order is classified the basis as.The result shows: bacterial strain ZJ-9 and Paenibacillus polymyxa reach 99.9% homology.What combining form was learned and observed, Physiology and biochemistry is identified identification the present invention use is Paenibacillus polymyxa, is specially Paenibacillus polymyxa ZJ-9 (CGMCC No.3044).
4, nutritional character:
Do not need to add somatomedin in the substratum of Paenibacillus polymyxa ZJ-9, can utilize multiple compound as carbon source, these materials both can use separately, also can compositely in the proper ratio serve as carbon source.Organonitrogen or inorganic nitrogen can use as nitrogenous source.Each amounts of components is in the substratum: carbon source 40～100g/L, and nitrogenous source 6～15g/L, inorganic salt 0.1～10g/L, all the other are water.Carbon source generally adopts jerusalem artichoke inulin, glucose, wood sugar, fructose, lactic acid and glycerine etc., and nitrogenous source can adopt extractum carnis, peptone, yeast extract paste, corn steep liquor, soybean cake powder, cotton seed powder cake, urea and (NH
4)
2SO
4, NH
4Cl etc. also comprise the inorganic salts that sylvite, cobalt salt, sodium salt, calcium salt, magnesium salts, zinc salt, molysite, manganese salt, phosphoric acid salt, dihydrogen phosphate and hydrochloride etc. are commonly used in the substratum.
Utilize above-mentioned Paenibacillus polymyxa to prepare optical purity R, R type 2, the method of 3-butyleneglycol: the inoculation that with preserving number is CGMCC No.3044 is cultivated in the aseptic culture medium of carbonaceous sources, nitrogenous source, inorganic salt and water, fermentative production optical purity R, R type 2, the 3-butyleneglycol, wherein, described carbon source is the jerusalem artichoke inulin without any hydrolysis treatment.
Wherein, in the described substratum, each amounts of components is: carbon source 40～100g/L, and nitrogenous source 6～15g/L, inorganic salt 0.1～10g/L, all the other are water.
Wherein, in the substratum, described nitrogenous source is extractum carnis, peptone, yeast extract paste, corn steep liquor, soybean cake powder, cottonseed meal, urea, (NH
4)
2SO
4And NH
4Among the Cl one or more; Described inorganic salt are one or more in sylvite, cobalt salt, sodium salt, calcium salt, magnesium salts, zinc salt, molysite, manganese salt, phosphoric acid salt, dihydrogen phosphate and the hydrochloride.
Wherein, be that the bacterial strain of CGMCC No.3044 carries out culture condition and is with preserving number: initial pH scope is 5.0～7.5, and culture temperature is 28～39 ℃, ventilate, and stir culture, incubation time is 48～84 hours.
Specifically:
The present invention is to provide and utilize the jerusalem artichoke inulin one-step fermentation to prepare optical purity R, R type 2, the method of 3-butyleneglycol, be that Paenibacillus polymyxa ZJ-9 bacterial strain slant activation with preserving number CGMCC No.3044 is after one day (slant medium employing beef-protein medium), in the jerusalem artichoke inulin without any hydrolysis treatment is to cultivate 48～84 hours on sole carbon source, the substratum that adds nitrogenous source and inorganic salt, can generate 10～60g/L optical purity R, R type 2, the 3-butyleneglycol, optical purity reaches more than 98%.Preferred fermentation condition is: substratum: without the jerusalem artichoke inulin 40～100g/L of any hydrolysis treatment, corn steep liquor 5.9～10g/L, (NH
4)
2SO
40.1～5g/L, K
2HPO
40.1～5g/L; 28～39 ℃ of leavening temperatures, more preferably 28～32 ℃; The initial pH scope of substratum is 5.0～7.5, more preferably 5.4～6.3; Ventilation, stir culture 48～55 hours.
Beneficial effect: a kind of Paenibacillus polymyxa of the present invention and utilize its preparation optical purity R, R type 2, the method for 3-butyleneglycol compared with prior art have following advantage:
(1) the present invention screens a strain and directly utilizes jerusalem artichoke inulin product optical purity R, R type 2, the 3-butyleneglycol, this bacterium produces inulinase, can directly utilize jerusalem artichoke inulin without hydrolysis treatment as sole carbon source fermentative production optical purity R, R type 2, the 3-butyleneglycol, collection produces enzyme, saccharification and optical purity R, R type 2, the 3-butyleneglycol fermentation is the single stage method fermentation of one, and easy to operate simple, culture condition is very extensive.
(2) can use soybean cake powder (substituting yeast extract paste or peptone) as optical purity R, R type 2, the nitrogenous source of 3-butyleneglycol fermentation substratum declines to a great extent the fermentation raw material cost.
(3) utilize that production bacterium that the present invention screens and cultural method provided by the present invention produce 2, the 3-butyleneglycol is optical purity R, R type, optical purity reach more than 98%, have improved products production and have been worth and added value.
Embodiment:
According to following embodiment, the present invention may be better understood.Yet the described concrete material proportion of embodiment, processing condition and result thereof only are used to illustrate the present invention, make those skilled in the art will readily understand, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
Slant medium: peptone 10g/L, extractum carnis 3g/L, NaCl 5g/L, agar 20g/L, pH7.0.
Seed culture medium: jerusalem artichoke inulin 20g/L, peptone 5g/L, yeast extract paste 5g/L, NaCl5 g/L, pH7.0.
Fermention medium: jerusalem artichoke inulin 40g/L, extractum carnis 6g/L, K
2HPO
41.5g/L, MgSO
47H
2O 0.3g/L, CaCl
20.3g/L pH 6.0; 250mL triangular flask liquid amount 50mL sterilized 30 minutes for 115 ℃.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, cultivate 24h for 30 ℃ and get seed liquor, the inoculum size of seed liquor by fermentating liquid volume 4% inserted in the cooled fermented liquid, 30 ℃, 120rpm/min cultivates 48h, contains optical purity R in the fermented liquid, R type 2,3-butyleneglycol 19.7g/L, optical purity reaches 99%.
Embodiment 2:
Slant medium and seed culture medium are with embodiment 1.
Fermention medium: jerusalem artichoke inulin 40g/L, extractum carnis 3g/L, (NH
4)
2SO
44g/L, K
2HPO
41.75g/L, KH
2PO
42.9g/L, MgSO
47H
2O 0.05g/L, FeSO
47H
2O 0.01g/L, ZnSO
47H
2O 0.001g/L, MnSO
47H
2O 0.05g/L, CaCl
20.3g/L pH 7.0.250mL triangular flask liquid amount 50mL sterilized 30 minutes for 115 ℃.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, cultivate 24h for 30 ℃ and get seed liquor, the inoculum size of seed liquor by fermentating liquid volume 4% inserted in the cooled fermented liquid, 37 ℃, 120rpm/min cultivated 50 hours, contained optical purity R in the fermented liquid, R type 2,3-butyleneglycol 19.5g/L, optical purity reaches 98%.
Embodiment 3:
Slant medium and seed culture medium are with embodiment 1.
Fermention medium: jerusalem artichoke inulin 100g/L, yeast extract paste 6g/L, peptone 6g/L, (NH
4)
2SO
43g/L, K
2HPO
44g/L, KH
2PO
41g/L, MgSO
47H
2O 0.3g/L, CaCl
20.3g/L, Co (NO
3)
20.04g/L, pH5.0.500mL triangular flask liquid amount 100mL sterilized 30 minutes for 115 ℃.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, cultivate 24h for 30 ℃ and get seed liquor, the inoculum size of seed liquor by fermentating liquid volume 4% inserted in the cooled fermented liquid, 35 ℃, 120rpm/min cultivated 48 hours, contain optical purity R in the fermented liquid, R type 2,3-butyleneglycol 41.8g/L, optical purity reaches 98%.
Embodiment 4:
Slant medium and seed culture medium are with embodiment 1.
Fermention medium: jerusalem artichoke inulin 60g/L, corn steep liquor 10g/L, (NH
4)
2SO
42g/L, K
2HPO
44g/L, pH 6.0; Liquid amount 100mL in the 500mL volumetrical triangular flask sterilized 30 minutes for 115 ℃.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, cultivate 24h for 30 ℃ and get seed liquor, the inoculum size of seed liquor by fermentating liquid volume 4% inserted in the cooled fermented liquid, 30 ℃, after 120rpm/min cultivates 55h, optical purity R in the fermented liquid, R type 2, the content of 3-butyleneglycol is 28.6g/L, and optical purity reaches 99%.
Embodiment 5:
Slant medium and seed culture medium are with embodiment 1.
Fermention medium: in the inulin crude extract of 40g/L, 3L, add 18g corn steep liquor, 10g (NH
4)
2SO
4, 7.5g K
2HPO
4, pH 6.0, are made into fermented liquid, in the glass stirred fermentor of the 5L that packs into, and 115 ℃ of steam sterilizings 30 minutes.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, 30 ℃, 120rpm/min cultivates 24h and gets seed liquor, the inoculum size of seed liquor by fermentating liquid volume 5% inserted in the cooled fermented liquid, 30 ℃ of cultivations (ventilation 0.5vvm, mixing speed 160rpm/min) 50h.Contain optical purity R in the fermented liquid, R type 2,3-butyleneglycol 19.4g/L, optical purity reaches 99%.
Embodiment 6:
Slant medium and seed culture medium are with embodiment 1.
Fermention medium: with 420g soybean cake powder, 60g K
2HPO
4, 10.5g MgSO
47H
2O and 10.5g CaCl
2, join in the inulin crude extract of 40g/L, 35L, regulating pH is 6.0, in the 50L mechanical agitating fermentation tank of packing into, 115 ℃ of steam sterilizings 30 minutes.
With preserving number is that the Paenibacillus polymyxa ZJ-9 of CGMCC No.3044 connects two and encircles in seed culture medium, 30 ℃, 120rpm/min cultivates 24h and gets seed liquor, and the inoculum size of seed liquor by fermentating liquid volume 5% inserted in the cooled fermented liquid, cultivates 48h for 30 ℃.Contain optical purity R in the fermented liquid, R type 2,3-butyleneglycol 18.2g/L, optical purity reaches 98%.
SEQUENCE?LISTING
＜110〉Yancheng Institute Of Technology
＜120〉a kind of Paenibacillus polymyxa and utilize its preparation optical purity R, R type 2, the method for 3-butyleneglycol
<130>YIT090512
<160>1
<170>PatentIn?version?3.3
<210>1
<211>1498
<212>DNA
＜213〉Paenibacillus polymyxa (paenibacillus polymyxa)
<400>1
agagtttgat?cctggctcag?attgaacgct?ggcggcaggc?ctaacacatg?caagtcgagc 60
ggtagagaga?agcttgcttc?tcttgagagc?ggcggacggg?tgagtaaagc?ctaggaatct 120
gcctggtagt?gggggataac?gttcggaaac?ggacgctaat?accgcatacg?tcctacggga 180
gaaagcaggg?gaccttcggg?ccttgcgcta?tcagatgagc?ctaggtcgga?ttagctagtt 240
ggtgaggtaa?tggctcacca?aggcgacgat?ccgtaactgg?tctgagagga?tgatcagtca 300
cactggaact?gagacacggt?ccagactcct?acgggaggca?gcagtgggga?atattggaca 360
atgggcgaaa?gcctgatcca?gccatgccgc?gtgtgtgaag?aaggtcttcg?gattgtaaag 420
cactttaagt?tgggaggaag?ggcattaacc?taatacgtta?gtgttttgac?gttaccgaca 480
gaataagcac?cggctaactc?tgtgccagca?gccgcggtaa?tacagagggt?gcaagcgtta 540
atcggaatta?ctgggcgtaa?agcgcgcgta?ggtggtttgt?taagttggat?gtgaaatccc 600
cgggctcaac?ctgggaactg?cattcaaaac?tgactgacta?gagtatggta?gagggtggtg 660
gaatttcctg?tgtagcggtg?aaatgcgtag?atataggaag?gaacaccagt?ggcgaaggcg 720
accacctgga?ctaatactga?cactgaggtg?cgaaagcgtg?gggagcaaac?aggattagat 780
accctggtag?tccacgccgt?aaacgatgtc?aactagccgt?tgggagcctt?gagctcttag 840
tggcgcagct?aacgcattaa?gttgaccgcc?tggggagtac?ggccgcaagg?ttaaaactca 900
aatgaattga?cgggggcccg?cacaagcggt?ggagcatgtg?gtttaattcg?aagcaacgcg 960
aagaacctta?ccaggccttg?acatccaatg?aactttctag?agatagattg?gtgccttcgg 1020
gaacattgag?acaggtgctg?catggctgtc?gtcagctcgt?gtcgtgagat?gttgggttaa 1080
gtcccgtaac?gagcgcaacc?cttgtcctta?gttaccagca?cgtaatggtg?ggcactctaa 1140
ggagactgcc?ggtgacaaac?cggaggaagg?tggggatgac?gtcaagtcat?catggccctt 1200
acggcctggg?ctacacacgt?gctacaatgg?tcggtacaga?gggttgccaa?gccgcgaggt 1260
ggagctaatc?ccacaaaacc?gatcgtagtc?cggatcgcag?tctgcaactc?gactgcgtga 1320
agtcggaatc?gctagtaatc?gcgaatcaga?atgtcgcggt?gaatacgttc?ccgggccttg 1380
tacacaccgc?ccgtcacacc?atgggagtgg?gttgcaccag?aagtagctag?tctaaccttc 1440
gggaggacgg?ttaccacggt?gtgattcatg?actggggtga?agtcgtaaca?aggtaacc 1498
Claims (6)
1, a kind of Paenibacillus polymyxa, its classification called after Paenibacillus polymyxa (paenibacillus polymyxa) ZJ-9 has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and its preserving number is: CGMCCNo.3044.
2, utilize the described Paenibacillus polymyxa of claim 1 to prepare optical purity R, R type 2, the method of 3-butyleneglycol, it is characterized in that with preserving number being that the inoculation of CGMCC No.3044 is cultivated in the aseptic culture medium of carbonaceous sources, nitrogenous source, inorganic salt and water, fermentative production optical purity R, R type 2,3-butyleneglycol, wherein, described carbon source is the jerusalem artichoke inulin without any hydrolysis treatment.
3, the Paenibacillus polymyxa that utilizes according to claim 2 prepares optical purity R, R type 2, the method for 3-butyleneglycol, it is characterized in that in the described substratum, each amounts of components is: carbon source 40～100g/L, nitrogenous source 6～15g/L, inorganic salt 0.1～10g/L, all the other are water.
4, prepare optical purity R according to claim 2 or the 3 described Paenibacillus polymyxas that utilize, R type 2, the method of 3-butyleneglycol is characterized in that in the substratum, and described nitrogenous source is extractum carnis, peptone, yeast extract paste, corn steep liquor, soybean cake powder, cottonseed meal, urea, (NH
4)
2SO
4And NH
4Among the Cl one or more.
5, prepare optical purity R according to claim 2 or the 3 described Paenibacillus polymyxas that utilize, R type 2, the method of 3-butyleneglycol, it is characterized in that in the substratum that described inorganic salt are one or more in sylvite, cobalt salt, sodium salt, calcium salt, magnesium salts, zinc salt, molysite, manganese salt, phosphoric acid salt, dihydrogen phosphate and the hydrochloride.
6, the Paenibacillus polymyxa that utilizes according to claim 2 prepares optical purity R, R type 2, the method of 3-butyleneglycol, it is characterized in that with preserving number being that the bacterial strain of CGMCC No.3044 carries out culture condition and is: initial pH scope is 5.0～7.5, culture temperature is 28～39 ℃, ventilate, stir culture, incubation time is 48～84 hours.
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| CN102643869A (en) * | 2012-05-04 | 2012-08-22 | 天津科技大学 | Method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues |
| CN103060255A (en) * | 2013-01-24 | 2013-04-24 | 盐城工学院 | Genetically engineering bacterium capable of producing S-3-hydroxy-butanone as well as construction method and application thereof |
| CN103361296A (en) * | 2013-08-09 | 2013-10-23 | 天津大学 | Bacillus subtilis strain for producing high-purity chiral D-(-)-2,3-butanediol, and construction and applications |
| CN103614419A (en) * | 2013-11-29 | 2014-03-05 | 内蒙古科技大学 | Method for preparing 2,3-butanediol by using lemna minor as raw material |
| CN103614328A (en) * | 2013-12-04 | 2014-03-05 | 天津大学 | Paenibacillus polymyxa producing 2, 3-butanediol and use thereof |
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| CN104830851A (en) * | 2015-05-11 | 2015-08-12 | 盐城工学院 | Recombinant bacterium of formate dehydrogenase and application of recombinant bacterium |
| CN106086088A (en) * | 2016-07-20 | 2016-11-09 | 盐城工学院 | A kind of method utilizing bacillus polymyxa solid fermentation to produce 2,3 butanediols |
| CN107267560A (en) * | 2017-07-26 | 2017-10-20 | 盐城工学院 | One kind produces R, the method for the butanediols of R 2,3 using vinegar grain |
| CN107354178A (en) * | 2017-07-20 | 2017-11-17 | 盐城工学院 | A kind of method added amino acid and promote the butanediol of Microbe synthesis 2,3 |
| CN111073837A (en) * | 2019-12-31 | 2020-04-28 | 盐城工学院 | Fermentation method for promoting Paenibacillus polymyxa to produce spores |
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- 2009-06-01 CN CN2009100268071A patent/CN101565683B/en not_active Expired - Fee Related
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| CN102643869A (en) * | 2012-05-04 | 2012-08-22 | 天津科技大学 | Method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues |
| CN102643869B (en) * | 2012-05-04 | 2014-04-16 | 天津科技大学 | Method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues |
| CN103060255A (en) * | 2013-01-24 | 2013-04-24 | 盐城工学院 | Genetically engineering bacterium capable of producing S-3-hydroxy-butanone as well as construction method and application thereof |
| CN103060255B (en) * | 2013-01-24 | 2014-12-03 | 盐城工学院 | Genetically engineering bacterium capable of producing S-3-hydroxy-butanone as well as construction method and application thereof |
| CN103361296A (en) * | 2013-08-09 | 2013-10-23 | 天津大学 | Bacillus subtilis strain for producing high-purity chiral D-(-)-2,3-butanediol, and construction and applications |
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| CN112501071B (en) * | 2020-12-15 | 2022-04-22 | 河北省科学院生物研究所 | Paenibacillus polymyxa SWGC4112 and culture method and application thereof |
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