CN101563094A - Process for harvesting plants of the apocynaceae family - Google Patents
Process for harvesting plants of the apocynaceae family Download PDFInfo
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Abstract
Process for harvesting plants from the Apocynaceae family comprising the steps of : (a) removing the plants from the soil, (b) leaving the intact plants to cure for a period of at least 1 day, (c) cutting up the cured plants, (d) further drying the cut plants, to obtain dried plant material comprising the steroidal glycoside having the formula (2).
Description
Technical field
The present invention relates generally to the steroid glucoside field.More particularly, it relates to the results Apocynaceae, is also referred to as the method for asclepiadaceae plant, and this plant contains and has the active steroid glucoside of appetite inhibiting and for example can be used in the body weight control product.The invention particularly relates to results belong to (the inferior and inferior arhat in former name fire ground belongs to (Trichocaulon)) from fire ground inferior (Hoodia) plant.
Background of invention
Can be also referred to as asclepiadaceae available from Apocynaceae, particularly the extract of the plant of fiery ground subgenus (former name fire ground subgenus and inferior arhat belong to) has shown that having the appetite inhibiting activity also may can be used in the body weight control product.US 6,376, and 657 (CSIR) disclose these extracts and contained the steroid glucoside with formula 1:
Wherein
The R=alkyl;
R
1=H, alkyl, crotonyl (tiglyol), benzoyl or any other organic ester group;
R
2=H or one or more 6-deoxidation carbohydrates, or one or more 2,6-dideoxy carbohydrate or glucose molecule or its combination; And wherein dotted line shows at optional other key that exists between carbon atom C4 and the C5 or between carbon atom C5 and C6.
Have one of bioactive molecule in active one of the part of purifying of good appetite inhibiting and it is found that it is chemical compound with formula 2:
US 6,376,657 also disclose the method for extracting the steroid glucoside with formula 1 from the asclepiadaceae plant, comprise with the solvent processing vegetable material having the active part of appetite inhibiting with extraction, make and extract solution and separate, from extract solution, remove and desolvate and reclaim extract with the residue vegetable material.This patent also discloses the method for synthetic various steroid glucoside.
WO2005/116049 (Unilever) discloses steroid glucoside can be separated from Herba seu radix metaplexis (fire ground is inferior) section's vegetable material or with the not desirable components that exists in Herba seu radix metaplexis (fire ground is inferior) section's vegetable material by liquid state or supercritical carbon dioxide extraction.To grind to form fine powder and extraction subsequently from the dried plant material of Hoodia gordonii (Hoodia gordonii).
US 2005/0202103 discloses the Babalus bubalis L. palm and has belonged to extract, and the gas first portion of wherein Babalus bubalis L. being slapped platymiscium is dry (on cemented platform) in the shade.
US 7,008,648 disclose the method that obtains vegetable material from leopard Pittosporum and Orbea platymiscium, wherein be applicable to and dry hot air drying or lyophilization then dry comprising with the method for grinding original biomass, for example, lyophilization perhaps is cut into these biomass for example 2-10 centimetre small pieces, hot air drying or lyophilization then.
Purify and separates the appetite inhibiting steroid glucoside from apocynaceae plant, those of formula 2 especially are expensive and bother.Therefore desirable is to improve steroid glucoside, especially has the yield of the steroid glucoside of formula 2.
The inventor finds now surprisingly, can be by harvesting method raising of the present invention some steroid glucoside from apocynaceae plant, the yield that particularly has the steroid glucoside of formula (2), the method is characterized in that after plant is shifted out from soil, they are shelved air-dryly be lower than 90 weight % to moisture, with air-dry plant chopping and further dry to obtain to comprise the have formula dried plant material of steroid glucoside of (2):
The invention definition
Realize above-mentioned and other purpose by the present invention, it comprises the method for gathering in the crops apocynaceae plant in first aspect, comprises the following steps:
(a) plant is shifted out from soil,
(b) complete plant shelved air-dryly be lower than 90 weight % to moisture,
(c) with air-dry plant chopping,
(d) further the plant of dry chopping to obtain to comprise the have formula dried plant material of steroid glucoside of (2):
In second aspect, the present invention relates to obtain and to comprise according to said method the dried plant material of steroid glucoside of formula 2 of the amount of at least 0.095 weight %.
In the third aspect, the present invention relates to comprise the extract and the food of steroid glucoside with formula 2.
Detailed Description Of The Invention
Except in embodiment and Comparative Examples, or clearly indicate separately outside the part, all numerical value of describing quantity of material or reaction condition, material physical properties and/or purposes in this description all are understood as with word " approximately " to be modified.
Be noted that when indicating any scope of concentration or amount any specific higher concentration can combine with any specific low concentration or amount.
For avoiding query, word " comprises " and is meant " comprising ", but not necessarily " by ... constitute " or " by ... form ".In other words, listed step or option need not be exhaustive.
" chopping " used herein is meant that the plant size reduces, and comprises grinding, pulverizing etc.
" on average " used herein is meant the average steroid glucoside content of at least 10 different plants of selecting at random.
" air-dry " used herein is meant that the complete plant of simply shelving results thus reaches the process of the moisture that is lower than 90 weight % up to them.
A first aspect of the present invention is the results Apocynaceaes, more preferably the method for fire ground subfamily plant.This plant especially is preferably selected from Trichocaulon piliferum, Trichocaulon officinale, Hoodia currorii, Hoodia gordonii, Hoodia lugardii and composition thereof.Hoodia gordonii is especially preferred.
In the first step of this harvesting method, apocynaceae plant is shifted out from the soil of its growth fully, preferably include root.This can be with hands by extracting plant on ground (may by for example scoop) and carry out or using suitable harvester or instrument to carry out in automatization's mode.
In second step, complete plant is air-dry, that is, plant is simply shelved, reach up to them and be lower than 90 weight %, preferably be lower than 88 weight %, more preferably less than 83 weight % or even be lower than the moisture of 80 weight %.When plant is shelved air-dry time enough, realize the reduction of moisture automatically.It is found that air-dry time should be at least one day, but preferably at least three days.Maximum duration is generally 150 days.Preferred time quantum is 3 to 100 days, and more preferably 5 days to 50 days, the best was 7 to 30 days.
By simply complete plant being stayed on the soil that they are shifted out, carry out air-dry easily.Need not additional means and obtain the reduction of moisture and the surprising relative raising of the steroid glucoside amount that is accompanied by with formula 2.Air-dryly also can on the zone that covers with sunshade net for example, carry out, or in the building that is not subjected to any climatic effect, carry out.
After air-dry step, subsequently with air-dry plant chopping.This plant can be cut into Any shape, as cube, thin slice, julliene etc., as long as one of dimension preferably less than 20 millimeters, is more preferably less than 15 millimeters less than 30 millimeters.Therefore for thin slice or julliene shape, thickness should be less than these sizes, and for cube, all dimensions all should be less than these sizes, or the like.Can use traditional cutting equipment, as hog, rotating disk chipper or standard food cutting device, the machine that provides of Urschell for example, thus form the plant particles of chopping.Size is more little, and drying time subsequently is fast more, thereby reduces the probability of growth of microorganism.For cutting step and drying steps subsequently, can use whole strain, but preferred use not with the plant of root so that the minimizing possibility of microbial contamination.
Subsequently, the plant of shredding further is dried to much lower moisture, is preferably reducing under the condition that is directly exposed under the ultraviolet as far as possible.Suitable drying equipment comprises directly and the indirect air exsiccator according to the present invention, wherein uses the energy (for example electricity, gas, paraffin, the energy etc.) heated air of any kind.Solar energy sun heated air; Hot-air can be blasted subsequently in the stove that dry this material uses." drying " used herein can comprise lyophilization.
Usually, at 35 to 120 ℃,, most preferably carry out further drying under 60 to 100 ℃ for having preferred 50 to 100 ℃ of optimum drying time.The typical dry phase is generally at least 1 hour, the highlyest can be preferably 1 to 72 hour to two weeks, most preferably 3 to 48 hours.
In second drying steps, usually with the plant drying of chopping to being lower than 15 weight %, preferably be lower than 10 weight %, more preferably less than the residual moisture content of 5 weight %.Can measure (remnants) moisture with standard weights analytical technology or Ka Er Karl Fischer titration.
The gained dried plant material that is preferably small pieces or sheet form has at least 1.3 weight %, the average total content of steroid glucoside of preferred at least 1.6 weight %.The dried plant material that can obtain according to the present invention comprises at least 0.095 weight % (by meansigma methods), preferred at least 0.1 weight % or even surpass the steroid glucoside of formula 2 of the amount of 0.15 weight %.
Can after extraction or dissolving, use the amount of the steroid glucoside of high performance liquid chromatography (HPLC) mensuration dried plant material Chinese style 2 with UV detection.With about 5 gram materials with the about 80 milliliters methanol eddies 1 hour of seething with excitement.Filter extract obtained and use the methanol wash solid material.The filtrate that merges and washing liquid transferred in 100 ml flasks and with methanol supply volume.1 milliliter of filtrate is evaporated to dry doubling reconstruct in 1 milliliter of acetonitrile/water (50/50v/v).
Under 220 nanometers, measure steroid glucoside by LC-UV.For this reason, 20 microlitre extracts are expelled to 5 micron particles are housed and are furnished with on 250 * 4.6 millimeters Zorbax RX-C8 analytical columns of 12.6 * 4.6 millimeters ZorbaxRX-C8 guard columns that the same fixed phase is housed.Make this column system remain on 40 ℃.With 41.2% acetonitrile/methanol (85/15v/v) and 58.8% water/methanol (85/15v/v) beginning, under 1 ml/min flow velocity, carry out gradient elution.Initial condition kept 10 minutes, rose to 88.2% acetonitrile/methanol (85/15v/v) and 11.8% water/methanol (85/15v/v) through 30 minutes linearities then.After in the end keeping 5 minutes, with this system's reequilibrate to initial condition.
Use the chemical compound (using 95% in this case) of the formula 2 of any known purity to calibrate.Chemical compound 2 can use preparative liquid chromatography to separate from dry Hoodia gordonii extract, maybe can synthesize (referring to for example United States Patent (USP) 6,376,657, it is incorporated herein by this reference).In acetonitrile/water (1/1v/v), prepare the liquid storage of 100 mcg/ml and under 75,50,20,10 and 5 mcg/ml, prepare further diluent to produce the additional calibration standard specimen.UV response control compound 2 lubber-lines of use under 220 nanometers quantize.
In the further embodiment of method of the present invention, from the dried plant material, extract one or more steroid glucoside.Can use any extraction method.For example, can extract described in 657 as the US 6,376 that is incorporated herein by this reference.The solvent of specifically mentioning that is used for extracting is one or more of carrene (dichloromethane), water, methanol, hexane, ethyl acetate or its mixture.Perhaps, can use liquid state or supercritical carbon dioxide extraction steroid glucoside described in WO 2005/116049 (Unilever).
This dried plant material or its extract can be used in the appetite inhibiting food, and this constitutes a third aspect of the present invention.The example of this based food is beverage, snack, food bar (bar), coating, flavoring agent, soup etc., or for meal, they can be used for the diet control of Weight management or obesity.
Unless indicate separately, all amounts used herein, umber, ratio and percentage ratio are all by weight.
Although above-outlined the present invention, it should be apparent to those skilled in the art that and can under the situation that does not deviate from as described herein and claimed scope and spirit of the present invention, modify, change and change.Further illustration the present invention in following non-limiting examples now.
Embodiment 1
Gather in the crops the small-sized Hoodia gordonii plant of 0.5 kilogram of 28 average weight.Gather in the crops the medium-sized Hoodia gordonii plant of 1.4 kilograms of 138 average weights.They are divided into 2 groups at random, and the S group contains 50 plants, and the O group contains 78 plants.To cut into pieces and dry from 10 small-sized, large-scale and medium-sized plants of O group.To cut into pieces and dry from 10 medium-sized plants of S group.
All other plants at first " are dried " different number of days, maximum 60 days.After air-dry, measure the fresh weight % of all plants.To cut into pieces and dry from small-sized, the large-scale and medium-sized plant of O group then.To cut into pieces and dry from the medium-sized plant of S group.
Measure the content of chemical compound in all plants (2).
Table 1 has shown the initial weight of all plants, weight and chemical compound (2) content after air-dry.We find surprisingly, exist relatedly between the air-dry loss in weight mark that causes (hereinafter being defined as " Fraction ") and active quantities, show air-dry useful influence to chemical compound (2) content.
Fraction=(initial weight-final weight)/initial weight
Final weight is the weight after air-dry in this article.Provide this association by following equation:
Chemical compound (2)=exp (A+B* (1-Fraction))
Original water content is generally 90%.In the case, above-mentioned correlation (wherein Fraction changes the % moisture after air-dry into) can be revised as:
Chemical compound (2)=exp (A+B* (10/ (100-% moisture))
Table 2 has shown for small-sized, medium-sized group O, medium-sized group of S and macrophyte, and the value of A and B comprises their standard error and the P value of this correlation.
Table 1
The initial weight final weight
(kg) (kg) Fraction chemical compound (2)
Large-scale by 3.93 3.93 0.000 0.078
Large-scale by 6.01 6.01 0.000 0.098
Large-scale by 6.40 6.40 0.000 0.070
Large-scale by 4.55 4.55 0.000 0.089
Large-scale by 2.02 2.02 0.000 0.092
Large-scale by 3.50 3.50 0.000 0.062
Large-scale by 3.66 3.66 0.000 0.065
Large-scale by 4.35 4.35 0.000 0.153
Large-scale by 6.47 6.47 0.000 0.052
Large-scale by 3.85 3.85 0.000 0.054
Large-scale by 4.96 2.52 0.492 0.124
Large-scale by 2.63 0.48 0.819 0.100
Large-scale by 4.53 2.35 0.481 0.156
Large-scale by 5.97 0.47 0.921 0.121
Large-scale by 7.94 0.39 0.951 0.136
Large-scale by 4.73 2.34 0.505 0.146
Large-scale by 4.98 2.49 0.500 0.095
Large-scale by 3.35 0.46 0.864 0.165
Large-scale by 6.98 3.35 0.520 0.133
Large-scale by 4.67 2.22 0.525 0.147
Large-scale by 5.32 0.22 0.959 0.279
Large-scale by 3.11 0.16 0.948 0.193
Large-scale by 5.73 0.16 0.972 0.177
Large-scale by 4.85 0.21 0.957 0.228
Dali 2.90 0.16 0.944 0.161
Large-scale by 3.78 0.87 0.770 0.221
Large-scale by 3.97 1.06 0.733 0.089
Large-scale by 3.90 0.90 0.769 0.134
Large-scale by 4.67 1.25 0.732 0.129
Large-scale by 4.55 0.20 0.956 0.115
Medium-sized, O organizes 2.89 2.89 0.000 0.156
Medium-sized, O organizes 2.37 2.37 0.000 0.248
Medium-sized, O organizes 1.79 1.79 0.000 0.107
Medium-sized, O organizes 1.65 1.65 0.000 0.071
Medium-sized, O organizes 1.77 1.77 0.000 0.066
Medium-sized, O organizes 1.62 1.62 0.000 0.061
Medium-sized, O organizes 1.66 1.66 0.000 0.062
Medium-sized, O organizes 1.80 1.80 0.000 0.114
Medium-sized, O organizes 0.69 0.69 0.000 0.182
Medium-sized, O organizes 1.57 1.57 0.000 0.096
Medium-sized, O organizes 1.70 1.31 0.229 0.079
Medium-sized, O organizes 1.29 0.92 0.285 0.037
Medium-sized, O organizes 1.38 1.06 0.231 0.043
Medium-sized, O organizes 0.78 0.60 0.227 0.048
Medium-sized, O organizes 1.42 1.09 0.232 0.098
Medium-sized, O organizes 0.93 0.72 0.226 0.071
Medium-sized, O organizes 1.04 0.78 0.249 0.098
Medium-sized, O organizes 0.86 0.66 0.231 0.076
Medium-sized, O organizes 1.59 1.21 0.239 0.115
Medium-sized, O organizes 1.49 1.15 0.227 0.096
Medium-sized, O organizes 1.24 0.74 0.402 0.119
Medium-sized, O organizes 1.56 0.64 0.590 0.143
Medium-sized, O organizes 1.79 0.70 0.610 0.068
Medium-sized, O organizes 1.03 0.54 0.472 0.092
Medium-sized, O organizes 1.02 0.61 0.406 0.050
Medium-sized, O organizes 1.23 0.64 0.481 0.119
Medium-sized, O organizes 1.02 0.50 0.507 0.192
Medium-sized, O organizes 0.95 0.40 0.579 0.140
Medium-sized, O organizes 0.89 0.46 0.483 0.082
Medium-sized, O organizes 1.74 0.90 0.483 0.128
Medium-sized, O organizes 2.09 0.88 0.579 0.105
Medium-sized, O organizes 2.65 1.05 0.603 0.198
Medium-sized, O organizes 1.95 0.88 0.549 0.128
Medium-sized, O organizes 1.34 0.54 0.596 0.184
Medium-sized, O organizes 1.50 0.24 0.840 0.099
Medium-sized, O organizes 1.89 0.78 0.587 0.169
Medium-sized, O organizes 1.52 0.58 0.618 0.162
Medium-sized, O organizes 2.07 1.00 0.516 0.138
Medium-sized, O organizes 1.27 0.64 0.494 0.196
Medium-sized, O organizes 1.49 0.86 0.423 0.173
Medium-sized, O organizes 1.35 0.47 0.651 0.232
Medium-sized, O organizes 1.53 0.31 0.798 0.128
Medium-sized, O organizes 1.61 0.86 0.467 0.113
Medium-sized, O organizes 1.47 0.55 0.625 0.097
Medium-sized, O organizes 1.64 0.77 0.531 0.322
Medium-sized, O organizes 0.92 0.25 0.727 0.145
Medium-sized, O organizes 1.06 0.42 0.605 0.188
Medium-sized, O organizes 1.23 0.11 0.914 0.116
Medium-sized, O organizes 1.49 0.56 0.624 0.302
Medium-sized, O organizes 1.72 1.03 0.403 0.232
Medium-sized, O organizes 1.53 1.18 0.229 0.059
Medium-sized, O organizes 1.21 0.93 0.233 0.118
Medium-sized, O organizes 1.37 1.04 0.243 0.080
Medium-sized, O organizes 1.17 0.90 0.229 0.106
Medium-sized, O organizes 1.28 0.99 0.228 0.106
Medium-sized, O organizes 1.32 1.01 0.234 0.127
Medium-sized, O organizes 1.14 0.87 0.234 0.075
Medium-sized, O organizes 1.80 1.37 0.239 0.132
Medium-sized, O organizes 1.69 1.25 0.258 0.132
Medium-sized, O organizes 0.94 0.71 0.245 0.160
Medium-sized, O organizes 1.11 0.42 0.625 0.130
Medium-sized, O organizes 1.03 0.39 0.622 0.046
Medium-sized, O organizes 1.38 0.25 0.821 0.196
Medium-sized, O organizes 1.59 0.63 0.604 0.147
Medium-sized, O organizes 1.67 0.37 0.777 0.056
Medium-sized, O organizes 1.50 0.28 0.814 0.221
Medium-sized, O organizes 1.52 0.37 0.758 0.239
Medium-sized, O organizes 1.24 0.36 0.713 0.059
Medium-sized, O organizes 1.35 0.56 0.585 0.100
Medium-sized, O organizes 0.88 0.31 0.646 0.109
Medium-sized, O organizes 1.74 0.43 0.752 0.137
Medium-sized, O organizes 1.63 0.22 0.864 0.118
Medium-sized, O organizes 1.35 0.35 0.742 0.073
Medium-sized, O organizes 1.68 0.20 0.883 0.064
Medium-sized, O organizes 0.98 0.20 0.795 0.070
Medium-sized, O organizes 1.79 0.21 0.881 0.286
Medium-sized, O organizes 1.30 0.08 0.935 0.152
Medium-sized, O organizes 1.40 0.37 0.735 0.175
Medium-sized, O organizes 1.15 0.29 0.747 0.097
Medium-sized, O organizes 2.05 0.14 0.933 0.074
Medium-sized, O organizes 0.50 0.50 0.000 0.135
Medium-sized, O organizes 0.40 0.40 0.000 0.036
Medium-sized, O organizes 0.26 0.26 0.000 0.039
Medium-sized, O organizes 0.29 0.29 0.000 0.044
Medium-sized, O organizes 0.49 0.49 0.000 0.052
Medium-sized, O organizes 0.60 0.60 0.000 0.082
Medium-sized, O organizes 0.64 0.64 0.000 0.146
Medium-sized, O organizes 0.16 0.16 0.000 0.048
Medium-sized, O organizes 0.56 0.56 0.000 0.070
Medium-sized, O organizes 0.48 0.48 0.000 0.059
Medium-sized, O organizes 0.72 0.34 0.530 0.256
Medium-sized, O organizes 0.19 0.10 0.474 0.103
Medium-sized, O organizes 0.66 0.30 0.544 0.113
Medium-sized, O organizes 0.55 0.12 0.781 0.078
Medium-sized, O organizes 0.40 0.18 0.552 0.069
Medium-sized, O organizes 0.44 0.22 0.500 0.039
Medium-sized, O organizes 0.64 0.33 0.488 0.112
Medium-sized, O organizes 0.42 0.21 0.502 0.125
Medium-sized, O organizes 0.32 0.17 0.465 0.097
Medium-sized, O organizes 0.21 0.10 0.515 0.243
Medium-sized, O organizes 0.72 0.10 0.860 0.090
Medium-sized, O organizes 0.72 0.24 0.666 0.070
Medium-sized, O organizes 0.31 0.08 0.739 0.156
Medium-sized, O organizes 0.58 0.11 0.810 0.123
Medium-sized, O organizes 1.01 0.26 0.743 0.096
Medium-sized, O organizes 0.71 0.14 0.804 0.091
Medium-sized, O organizes 0.34 0.09 0.734 0.185
Medium-sized, O organizes 0.18 0.05 0.725 0.101
Medium-sized, S organizes 1.52 1.52 0.000 0.050
Medium-sized, S organizes 1.29 1.29 0.000 0.037
Medium-sized, S organizes 0.85 0.85 0.000 0.058
Medium-sized, S organizes 1.25 1.25 0.000 0.066
Medium-sized, S organizes 1.11 1.11 0.000 0.034
Medium-sized, S organizes 0.87 0.87 0.000 0.034
Medium-sized, S organizes 1.63 1.63 0.000 0.028
Medium-sized, S organizes 0.91 0.91 0.000 0.051
Medium-sized, S organizes 1.26 1.26 0.000 0.041
Medium-sized, S organizes 2.16 2.16 0.000 0.077
Medium-sized, S organizes 1.27 0.95 0.251 0.038
Medium-sized, S organizes 1.36 1.03 0.245 0.171
Medium-sized, S organizes 2.20 1.70 0.228 0.135
Medium-sized, S organizes 1.48 1.15 0.225 0.121
Medium-sized, S organizes 1.91 1.39 0.274 0.082
Medium-sized, S organizes 1.41 1.04 0.263 0.067
Medium-sized, S organizes 1.43 1.10 0.231 0.088
Medium-sized, S organizes 1.29 1.00 0.230 0.037
Medium-sized, S organizes 1.31 0.98 0.250 0.062
Medium-sized, S organizes 0.96 0.73 0.237 0.234
Medium-sized, S organizes 1.70 0.89 0.477 0.038
Medium-sized, S organizes 1.08 0.40 0.629 0.171
Medium-sized, S organizes 1.05 0.54 0.485 0.135
Medium-sized, S organizes 1.23 0.58 0.528 0.121
Medium-sized, S organizes 1.60 0.45 0.717 0.082
Medium-sized, S organizes 1.14 0.46 0.598 0.067
Medium-sized, S organizes 0.95 0.45 0.524 0.088
Medium-sized, S organizes 1.39 0.71 0.490 0.037
Medium-sized, S organizes 1.08 0.53 0.511 0.062
Medium-sized, S organizes 0.90 0.65 0.278 0.234
Medium-sized, S organizes 1.29 0.77 0.405 0.241
Medium-sized, S organizes 1.03 0.43 0.583 0.160
Medium-sized, S organizes 0.83 0.35 0.576 0.076
Medium-sized, S organizes 1.20 0.32 0.731 0.290
Medium-sized, S organizes 1.36 0.51 0.626 0.115
Medium-sized, S organizes 1.58 0.94 0.407 0.218
Medium-sized, S organizes 2.39 0.97 0.594 0.165
Medium-sized, S organizes 0.88 0.46 0.480 0.142
Medium-sized, S organizes 0.95 0.39 0.589 0.094
Medium-sized, S organizes 1.81 0.83 0.541 0.156
Medium-sized, S organizes 1.40 0.68 0.515 0.126
Medium-sized, S organizes 0.83 0.23 0.724 0.147
Medium-sized, S organizes 1.92 0.16 0.917 0.196
Medium-sized, S organizes 1.10 0.46 0.583 0.279
Medium-sized, S organizes 1.55 0.14 0.910 0.239
Medium-sized, S organizes 0.86 0.16 0.814 0.310
Medium-sized, S organizes 2.56 0.22 0.915 0.132
Medium-sized, S organizes 1.71 0.40 0.766 0.159
Medium-sized, S organizes 2.01 0.12 0.940 0.087
Medium-sized, S organizes 1.03 0.27 0.739 0.069
Small-sized 0.50 0.50 0.000 0.135
Small-sized 0.40 0.40 0.000 0.036
Small-sized 0.26 0.26 0.000 0.039
Small-sized 0.29 0.29 0.000 0.044
Small-sized 0.49 0.49 0.000 0.052
Small-sized 0.60 0.60 0.000 0.082
Small-sized 0.64 0.64 0.000 0.146
Small-sized 0.16 0.16 0.000 0.048
Small-sized 0.56 0.56 0.000 0.070
Small-sized 0.48 0.48 0.000 0.059
Small-sized 0.72 0.34 0.530 0.256
Small-sized 0.19 0.10 0.474 0.103
Small-sized 0.66 0.30 0.544 0.113
Small-sized 0.55 0.12 0.781 0.078
Small-sized 0.40 0.18 0.552 0.069
Small-sized 0.44 0.22 0.500 0.039
Small-sized 0.64 0.33 0.488 0.112
Small-sized 0.42 0.21 0.502 0.125
Small-sized 0.32 0.17 0.465 0.097
Small-sized 0.21 0.10 0.515 0.243
Small-sized 0.72 0.10 0.860 0.090
Small-sized 0.72 0.24 0.666 0.070
Small-sized 0.31 0.08 0.739 0.156
Small-sized 0.58 0.11 0.810 0.123
Small-sized 1.01 0.26 0.743 0.096
Small-sized 0.71 0.14 0.804 0.091
Small-sized 0.34 0.09 0.734 0.185
Small-sized 0.18 0.05 0.725 0.101
Table 2
Claims (20)
1. the method for results apocynaceae plant comprises the following steps:
(a) plant is shifted out from soil,
(b) complete plant shelved air-dryly be lower than 90 weight % to moisture,
(c) with air-dry plant chopping,
(d) further the plant of dry chopping to obtain to comprise the have formula dried plant material of steroid glucoside of (2):
2. method according to claim 1 was shelved this plant air-dry about at least 1 day thus.
3. according to each method of aforementioned claim, thus this plant was shelved air-dry about 1 to about 150 days.
4. according to each method of aforementioned claim, thus this plant was shelved air-dry about 3 to about 100 days.
5. according to each method of aforementioned claim, thus this plant was shelved in the open air-dry about 7 to about 30 days.
6. according to each method of aforementioned claim, wherein, subsequently with air-dry plant drying to the residual moisture content that is lower than about 15 weight %.
7. according to each method of aforementioned claim, wherein in about 35 ℃ of dryings of carrying out to about 120 ℃ temperature subsequently.
8. according to each method of aforementioned claim, wherein this plant is selected from Hoodia gordonii, Hoodia currorii, Hoodia Iugardii and composition thereof.
9. method according to Claim 8, wherein this plant is a Hoodia gordonii.
10. according to each method of aforementioned claim, wherein this dried plant material further comprises one or more other steroid glucoside with following formula:
R=alkyl wherein;
R
1=H, alkyl, crotonyl, benzoyl or any other organic ester group;
R
2=H or one or more 6-deoxidation carbohydrates or one or more 2,6-dideoxy carbohydrate or glucose molecule or its combination; And wherein dotted line shows optional between carbon atom C4 and the C5 or between carbon atom C5 and C6 and has other key, or its mixture.
11. according to each method of aforementioned claim, wherein this dried plant material has the average steroid glucoside total content based on the about at least 1.3 weight % of anhydrous dried plant material.
12. according to each method of aforementioned claim, wherein single dried plant material has the steroid glucoside total content based on the about at least 1.6 weight % of anhydrous dried plant material.
13. according to each method of aforementioned claim, wherein this dried plant material comprises the steroid glucoside based on the formula 2 of the about at least 0.095 weight % of anhydrous dried plant material.
14. can comprise steroid glucoside according to the dried plant material of each described method acquisition of aforementioned claim based on the formula 2 of the about at least 0.095 weight % of anhydrous dried plant material.
15. comprise the food of the dried plant material that can obtain according to each method of claim 1-13.
16., further comprise by this dried plant material obtaining extract according to each method of claim 1-13.
17. the extract that can obtain according to the method for claim 16.
18. comprise the food of the extract of claim 17.
19. according to the food of claim 18, it is the product form of eating in beverage, snack, food bar, coating, flavoring agent, soup or generation.
20. according to each method of aforementioned claim, wherein steroid glucoside is used for the diet control of Weight management or obesity.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06126853 | 2006-12-21 | ||
| EP06126853.8 | 2006-12-21 | ||
| EP07104335.0 | 2007-03-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101563094A true CN101563094A (en) | 2009-10-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007800472759A Pending CN101563094A (en) | 2006-12-21 | 2007-12-07 | Process for harvesting plants of the apocynaceae family |
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| CN (1) | CN101563094A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763840A (en) * | 2011-05-05 | 2012-11-07 | 吕胜一 | Infinite expansion method of food field and manufacturing equipment |
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2007
- 2007-12-07 CN CNA2007800472759A patent/CN101563094A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763840A (en) * | 2011-05-05 | 2012-11-07 | 吕胜一 | Infinite expansion method of food field and manufacturing equipment |
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