CN101553122A - Post-harvest treatment of bananas with a new antifungal composition - Google Patents
Post-harvest treatment of bananas with a new antifungal composition Download PDFInfo
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- CN101553122A CN101553122A CNA2007800453743A CN200780045374A CN101553122A CN 101553122 A CN101553122 A CN 101553122A CN A2007800453743 A CNA2007800453743 A CN A2007800453743A CN 200780045374 A CN200780045374 A CN 200780045374A CN 101553122 A CN101553122 A CN 101553122A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
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Abstract
The present invention relates to the treatment of wounds occurring from cutting bananas and pineapples from a tree with a composition containing natamycin and at least one phosphite containing compound.
Description
Invention field
The invention discloses a kind of new antimicrobial compositions, be used to prevent microbial spoilage after the results of banana and pineapple.
Background of invention
Estimating has about 25% owing to microbial spoilage loses in the world crop production, and wherein the corruption that causes of fungi is most important reason at present.No matter be from economic angle or from human angle, the corruption of prevention food product all is very important.After all, in a lot of places in the world still the someone suffer hunger.
In this respect, banana is important crop.Banana comes after paddy rice, wheat and the corn in human running stores, is the 4th.Banana plant is to belong to the herbaceous plant that Musa belongs to, and it surpasses 100 countries and planted global.Most of bananas are that local crowd consumes, but also have a large amount of bananas to be exported to for example U.S., Europe and Japanese.
Banana plant and banana fruit after results all to the mould sensitivity.The Panama disease that Fusarium oxysporum causes is to propagate the widest banana plant disease.In addition, Mycosphaerella fijiensis and Mycosphaerella musicola mould cause the disease of Banana Leaf---so-called black yellow leaf spot.
Among the WO 2005/074687, described a kind of new antifungal composition, it contains for example natamycin, is used to prevent the growth of these Sapromyceses on the banana plant of field.The invention of describing among the WO 2005/074687 provides protection field banana plant to keep out the solution of pathogenic moulds.
FR 2 732 191 discloses a kind of method, and described method is treated leaf spot (cercosporiose) by the antifungal composition that the towering certain applications to banana plant comprise phosethyl-Al and optional triazole antifungal agents.
The fungus growth on the banana plant of field, the banana corruption that results back mould causes also is the subject under discussion that people mainly pay close attention to.This so-called " rotten worry " is causing from the mycotic infection afterwards of stem (stem) cutting banana stem (hand).In this stage, mycotic spore is easy to enter wound by milk sample resin and surface liquid from the injury.Transportation and/or ripe later stage, spore can be sprouted, mould and then grow into banana stem, and make the banana corruption.Rotten worry may be caused by multiple fungal species.Most important fungal species is Fusarium pallidoroseum, Colletotrichummusae, Verticillium theobromae, Thielaviopsis paradoxa, Lasiodiplodiatheobromae, Deightoniella torulosa and Fusarium roseum.Rotten worry causes being used for local banana that consumes and the banana considerable damage that is used to export.
In order to prevent mold damage, after results, with antifungal composition to all outlet ports banana at least and much be used for the wound of the local banana that consumes and wound near gather in the crops post processing.The example of the fungicide of using in the current practice is thiazole, thiabendazole, benomyl, imidazoles (for example pressing down mould azoles) or its mixture.
EP 0 290 155 discloses the absorbent layer material that is used for to the application of plant wound, wherein comprises the elastic sheet of layer material, permeable layer, absorber material and fungicide (for example thiabendazole).
US 4,148, and 891 disclose the antifungal preparation that polyene antibiotic is dissolved in lower alcohol and rudimentary alkanoic acid, are used for prevention or the growth of mould fungus inhibition on agricultural products (for example banana).
Griffee and Pinegar (1974) have checked some kinds of fungicides in the processing to rotten worry.In addition, the pimaricin that is called as natamycin now is also included within this research.Known from a lot of other public publications, natamycin is very effective antifungal compound.The author points out that really natamycin has shown broad spectrum of activity.But they also draw conclusion: the activity of natamycin is not enough to produce the Satisfactory Control to mould on the banana.
Though carried out handling widely with the fungicide of using at present, spoilage problem still exists.Known: mould quite is easy to develop the resistance to antifungal compound, and some to cause the mould of banana corruption also be like this.After resistant strain development, will select, mould problems will increase, thereby will use further the more fungicide of a large amount.In addition, a lot of fungicides that are applied on the banana cause environmental pollution and human health problems.In addition, safety of workers also is a major issue, because known all farm labourerss will contact closely with these harmful fungicides of high concentration.
Pineapple belongs to Ananas and belongs to.This is the second important fruit after the banana, accounts for more than 20% of whole world tropical fruit (tree) output.Most of pineapple is consumed as fresh fruit in production country, but still has a considerable amount of pineapples also to be exported.The problem of the rotten worry of pineapple aspect is similar to banana.In addition, under the situation of pineapple, there is the wound of vulnerable for moulds after the results.Mould can with to infect pineapple at the similar mode of the described mode of banana, cause corruption and economic loss.
Therefore, can reach a conclusion, that people are starved of is more effective, more environmental friendliness, more hypotoxicity and the Antimicrobe compound/composition that is used to handle banana, pineapple and similar crop of harm (for example antifungal compound/composition) still less.
Invention is described
The invention provides antimicrobial (for example antimycotic) composition of new concertedness and by new compositions being applied to the technology that fruit is handled banana, pineapple and other crop, thereby solved the problems referred to above, described composition comprises polyene antifungal agent and at least a compound that contains orthophosphite.By using new antimicrobial compositions, can prevent mould and/or conk in banana, pineapple and other crop.In other words, new compositions of the present invention can protect banana, pineapple and other crop to keep out mould and/or conk and/or mould and/or fungal infection and/or fungi corruption.Advantageously, after the results composition is being applied on the crop, preferably, is showing on the crop of wound after being applied to results.In other words or, the results of crop are caused wound, and handle wound with composition of the present invention.Usually, after crop (for example fruit, for example banana or pineapple) was removed from the plant of its growth, wound had just produced.For example, the banana that cuts from banana plant has the wound that cutting causes.Cutting can wait with pocket knife, chopper, axe to be carried out.Can manually or automatically carry out.Therefore, usually, the crop of results (preferably fruit) links to each other with its plant by stalk (stalk), and this stalk will be destroyed by mechanical force.
Unexpectedly, the present inventor finds, when with polyene fungicide (for example natamycin) and the natural crop protection compound that belongs to the group of phosphite/ester (KH for example
2PO
3Or K
2HPO
3Or the mixture of these two kinds of phosphites) combination, and applied in any combination to the fruit wound the time, is able to remarkable enhancing to the protection of keeping out mould after for example banana and the pineapple harvesting.The compound that contains orthophosphite used herein represents to comprise orthophosphite group, i.e. PO
3(H for example
2PO
3 -, HPO
3 2-Or PO
3 3-Form) compound, comprise phosphorous acid and compounds such as phosphonic acids and derivative thereof, described derivative is its ester and/or alkali metal or alkali salt for example.
Therefore composition of the present invention comprises polyene fungicide and at least a compound that contains orthophosphite.In one embodiment, composition of the present invention comprises 0.1g or lignin still less for every gram polyene fungicide, more preferably, and 0.1g or polyphenol still less.Preferably, they comprise 0.01g or lignin still less for every gram polyene fungicide, more preferably, and 0.01g or polyphenol still less.Especially, they do not contain lignin, preferably do not contain polyphenol.
The suitable example that is applied to polyene fungicide of the present invention is natamycin, nystatin, amphotericin B, Philippine's mycin (filipin) and lucensomycin (lucensomycin).Preferred polyene fungicide is a natamycin.In one embodiment, composition can also contain two or more different polyene fungicides.Should be appreciated that the derivative of polyene fungicide, include but not limited to that the modified form of the salt of polyene fungicide or solvate or polyene fungicide also can be applicable to composition of the present invention.An example that contains the commercial product of natamycin is to have trade name
Product.
Be DSM Food Specialties (Holland) production, it contains the natamycin of 50% (w/w).This commercial product can be impregnated in the composition of the present invention.
The suitable example that contains the compound of orthophosphite is phosphorous acid and salt (alkali metal or alkali salt) thereof, for example, and potassium phosphite, for example KH
2PO
3And K
2HPO
3, (the C of sodium phosphite and ammonium phosphite and phosphorous acid
1-C
4) Arrcostab and salt thereof, for example, ethyl phosphorous acid aluminium (phosethyl-Al), ethyl phosphorous acid calcium, isopropyl phosphorous acid magnesium, isobutyl group phosphorous acid magnesium, sec-butyl phosphorous acid magnesium and N-butyl aluminium phosphite.The mixture that certainly, also can comprise the compound that contains orthophosphite.Can pass through for example with KOH or K
2CO
3Add KH
2PO
3Solution extremely whole pH is 5.0-6.0, easily obtains for example KH
2PO
3And K
2HPO
3Mixture.As noted above, also can in composition of the present invention, comprise the compound of precursor-type, their metabolism in crop or plant are phosphorons acid compound.The example of this compounds is phosphonate/ester, for example the phosethyl-Al compound.In for example crop or plant, the ethylphosphonic acid root portion metabolism of this molecule is an orthophosphite.An example of this compounds is business-like being called as
(Bayer, ethane phosphonic acid Germany) (ethylhydrogen phosphonate) product.
Composition of the present invention can have 4 to 8 pH, preferably, and 5 to 7 pH.They can be solids, and for example powder composition perhaps can be a liquid.Advantageously, they are can be by the liquid that for example banana or pineapple is soaked, spraying or electrostatic spray are used.Perhaps, also can use the brush that is impregnated with antifungal composition of the present invention or for example cotton-wool mat or cellulose mat are handled wound on banana or the pineapple.Also can use clay (plaster) the sample carrier that is put on the wound to use composition of the present invention.In addition, can use the resin-like or the wax sample composition that can cover wound surface known in the art.In another aspect of this invention, can use antimicrobial compositions by the fruit coating.Example as the compound of fruit coatings applications is xanthans, sugar, glycerine and wax, for example beeswax.
For all these processing, can use processing method and the equipment of well known to a person skilled in the art.The main benefit of using brush, clay sample composition and spraying resin in wound surface is, fungicide is applied directly in the wound tissue, but not on the whole fruit.On the other hand, the water-based of using automatic system to carry out is soaked or spray applications has reduced labor cost, has more cost benefit.
Composition of the present invention is used after results.Under the situation of banana, preferably after scaling off banana stem, directly using on stem.Under the situation that banana is cleaned, when banana takes out, can use composition of the present invention from rinse bath.Rinse bath is used for removing dust and field heat (fieldheat).Being put into specific going at banana removes milk (from the cutting handle wound seepage that causes) and prevents that thus milk from dripping under the situation that (may form ugly spot) on the banana in the milk groove, when banana takes out, can use composition of the present invention from remove the milk groove.Perhaps, composition of the present invention can add cleaning or go in the milk solution.Afterwards, handle can be cut to four to six refer to (banana) bunch, again it is cleaned.In addition, during this extra cleaning step or afterwards, also can use composition of the present invention.In one embodiment, the also available composition that comprises polyene fungicide (for example natamycin) is handled banana or pineapple, handles with comprising at least a compound compositions that contains orthophosphite afterwards, perhaps conversely.In addition, can be before or after with compositions-treated banana of the present invention, antimycotic and/or antimicrobial compositions is handled banana with other.
After antimicrobial compositions processing of the present invention, banana can be put in the plastic systems, for example put in the so-called miniature wet packet system (being generally used for being packaged in the outlet banana that has carried out immersion treatment in the antimicrobial compositions).Perhaps, banana can be by dress box and transportation.
Composition of the present invention also comprises concentrated stock suspensions/solutions and concentrated dry products, for example powder, particle and tablet.They can be used for preparing composition, to be used for submergence or spraying banana, pineapple or other crop.
Composition of the present invention can comprise the polyene fungicide of 0.05g/l to 100g/l usually, preferably, and 0.1g/l to 50g/l.Preferably, this amount is 0.1g/l to 3g/l.Preferably, polyene fungicide is a natamycin.Composition can comprise the potassium phosphite of 0.5g/l to 1000g/l usually, preferably, and the potassium phosphite of 1g/l to 500g/l.More preferably, the amount of potassium phosphite is 2g/l to 30g/l.According to the present invention, also can use other phosphite/ester with the potassium phosphite equimolar amounts.In one embodiment, the concentration of composition of the present invention Central Asia phosphate/ester, that is, and PO
3The concentration of group is between 1 to 1000mM, preferably between 10 to 750mM, more preferably between 25 to 500mM.
In addition, composition of the present invention also can contain at least a other antifungal compound, for example press down mould azoles (Imazalil, Janssen Pharmaceutica NV, Belgium), probenazole (Thiabendazole, Syngenta for example, the commercial product of USA
FlowableSC), benomyl (Benomyl), captan (Captan, non-systemic (nonsystemic) phthalimide fungicide), prochloraz (N-propyl group N-[2-(2,4,6-Trichlorophenoxy) ethyl] imidazoles-1-formamide) and formalin and with title
M (Cerexagri Inc, active component thiophanate-methyl), Jet-
(Certis Europe BV, The Netherlands, active component peracetic acid and hydrogen peroxide) and
(Syngenta, Switserland, active component fluazinam) known commercial product.Other suitable antifungal compound can be at Gewasbeschermingsgids2006, Gids voor gewasbescherming in de land-en tuinbouw en het openbaar enparticulier groen, Plantenziektenkundige Dienst, 2006,560 pages, Paperback, Gewasbeschermingsgids-ISSN 1571-201X finds among the Volume 18.
Composition of the present invention can advantageously contain at least a binding agent, and it promotes antifungal compound to be bonded to the surface of banana for example or pineapple fruit.This type of binding agent is based on the product of latex, for example,
(Holland Fyto B.V., The Netherlands) and
(LovelandIndustries Ltd), based on the product of Pilonene/ terpene Nu-for example
(Hygrotech Saad) and Spray-
(Mandops) and long-chain polysaccharide (for example xanthans, gellan gum and guar gum).Perhaps, binding agent can be the polymer or the copolymer of for example polyacrylate and polyethylene polymer type, for example
(DSM, The Netherlands).Composition of the present invention also can comprise two or more different binding agents.
For processing has the object of hydrophobic surface, for example banana or pineapple, adding at least a surfactant may be good.Therefore be also included within the present invention to the described compound of the optional adding of composition of the present invention.The example of useful surfactant is an anion surfactant, for example, and dodecyl sodium sulfate or polyethylene alkyl ether or polyoxy ether, for example
60,61 or 65.Other example of useful surfactant is organic silicone, sulfosuccinate/ester, alcohol ethoxylates, fatty acid ethoxy compound, fatty acid propoxylation compound and commercial product
(Asepta BV, The Netherlands).Therefore, in a kind of specific embodiment, composition of the present invention also can comprise extra compound, for example, surfactant, binding agent, the suitable carriers that is generally used for formula technique and adjuvant, these carriers and adjuvant include but not limited to mineral matter, solvent, dispersant, emulsifier, wetting agent, stabilizing agent, defoamer, buffer and antioxidant.
For improving the validity and the practical purposes of the present composition, also can in antifungal composition, add the compound of resisting insect, nematode, acarid and bacterium.The example of this compounds is
(Bayer), formalin and
(Syngenta, Switserland).
In addition, the invention provides the crop that crosses with compositions-treated of the present invention.Treated crop can contain the coating that comprises the present composition.In one embodiment, treated crop comprises 0.01 to 20mg/dm on its surface
2, preferred 0.1 to 10mg/dm
2Natamycin.In another embodiment, they comprise 0.1 to 600mg/dm on its surface
2, preferred 3 to 300mg/dm
2Potassium phosphite.According to the present invention, other compound that contains orthophosphite also can use, and treated crop can comprise on its surface with other of potassium phosphite equimolar amounts and contain the compound of orthophosphite.The example of this type of crop is a fruit, for example has other fruit of wound when banana, pineapple and results.Under the situation of banana, composition may be used on banana stem, banana stem, banana clusters or even single banana on.Therefore, these bananas " form " of crossing through compositions-treated of the present invention are also included within the present invention.In one embodiment, wound that pair fruit harvest causes is only arranged by compositions-treated of the present invention.Wound can be present on the stalk of banana, and in this case, only the stalk of banana is by compositions-treated of the present invention.
Embodiment
Embodiment 1
Processing to banana
In this experiment, use is from organic banana of the Dominica Republica
Obtain banana from the local retailer of Holland.Spore with plant pathogenic moulds Colletotrichum musae CBS19231 is handled banana.Use known method to obtain mycotic spore.Make the banana damaged according to the described method of de Lapere deBellaire and Dubois (1987).Use cork drill damage banana twice, then according to every place wound about 2.5 * 10
4The amount of individual C.musae CBS19231 mycotic spore is polluted.After at room temperature hatching 4 hours, banana was soaked in one of following composition 1 minute: a) non-processor (contrast 1), b) be soaked in (contrast 2) in the water, c) be soaked in the 1000ppm natamycin, d) be soaked in the 240mM potassium phosphite, e) be soaked in 1000ppm natamycin+240mM potassium phosphite.Experiment repeats three parts to be carried out, and uses known method to prepare natamycin and potassium phosphite solution.All compositions all contain the binding agent of 0.1% (v/v)
The surfactant of (Loveland Industries Ltd.) and 0.14% (v/v)
(Asepta BV, The Netherlands).After this processing, under 21 ℃ of humidity that raising, in seal box, hatch banana.By perusal banana being carried out development of moulds every day judges.
After 15 days, mould (have 6 in 6 wounds mould is arranged) is arranged all on two place's wounds of 3 bananas of two control groups (composition a and b).In addition, the banana of only using natamycin (composition c) and only using potassium phosphite (composition d) to handle demonstrates in 6 place's wounds whole 6 places respectively and has 5 places that fungus growth is arranged.But, in the banana of handling with the composition (composition e) that comprises natamycin and phosphite, in 6 place's wounds, only have 2 places to observe fungus growth.
Described result (seeing Table 1) clearlys show that in the attack test that uses high mould contamination to carry out, with natamycin or phosphite, the composition that comprises natamycin and phosphite compound can provide the protection of better keeping out mould to banana than only.Unexpectedly, the applied in any combination of natamycin and phosphite causes the strong synergistic that infects to reduce.
Embodiment 2
Processing to banana
The experiment that present embodiment is described is made at the stalk place of banana but wound is the usefulness pocket knife according to carrying out like that with embodiment 1 described experiment.
After hatching 15 days, all there is development of moulds all wounds of all wounds of two control groups (composition a and b) and the banana (composition d) handled through phosphite.In 3 bananas only using natamycin (composition c) to handle, there are 2 to observe fungus growth.In the banana of handling with the composition (composition e) that comprises natamycin and phosphite, only on 1 banana, observed fungus growth.
The clear expression of these results (seeing Table 2), in this attack test that uses high mould contamination, composition of the present invention also uses separately than only natamycin or phosphite and provides the protection of better keeping out mould to banana.
Embodiment 3
Handle the banana clusters of fresh results
In this experiment, use the banana of fresh results.Results and go milk to handle to remove after the milk of wound seepage several days with known method, described according to embodiment 1, with the mould spores infected banana and handle.For every kind of composition, use 3 bunches of bananas, wherein every bunch comprises 4 bananas (experiment repeats three parts and carries out).
After hatching in 15 days, with reference composition (composition a and b) handle bunch all bananas (12) and only use natamycin (composition c) or only use that phosphite (composition d) handles bunch all bananas all observed mould, and be soaked in the composition that comprises natamycin and phosphite (composition e) bunch 12 bananas in only 3 observed some fungus growths.
Use these results (seeing Table 3) of the banana acquisition of fresh results to clearly show that composition of the present invention can effectively protect banana to keep out mould.
Embodiment 4
Handle the banana of fresh results
The experiment that present embodiment is described still after the banana results, is made fresh wound (making 2 place's wounds for every bunch) with pocket knife according to carrying out like that with embodiment 3 described experiments on the stalk of banana clusters.
After hatching in 15 days, all developed mould on all banana clusters handled with reference composition (composition a and b) and the banana clusters of only using natamycin (composition c) to handle.Also there are 2 to observe serious fungus growth in 3 banana clusters only using phosphite (composition d) to handle.And only observed some not serious fungus growths on the banana clusters with the compositions-treated that comprises natamycin and phosphite (composition e).
This result of experiment (seeing Table 4) shows that banana stalk is effectively protected by composition of the present invention.
Embodiment 5
Processing to banana
Experiment described herein is according to embodiment 1 described carrying out.But in this experiment, do not use binding agent
And surfactant
Thing has instead used concentration to be respectively 21.3g/l and 1.0g/l
(DSM) and xanthans.In this experiment, make 12 place's wounds, replace 6 place's wounds (every banana 2 place's wounds damage 6 bananas).
After 15 days, all wounds of contrast banana all demonstrate fungus growth, and 50% of the banana wound that 25% and natamycin in the banana wound of handling with phosphite handled has shown fungus growth.And on the banana of handling with composition of the present invention (natamycin and phosphite), do not observe fungus growth after hatching in 15 days.
These results (seeing Table 5) clearly show that though the degree of mould contamination is extremely high in this experiment, incubation conditions also is that conk is the suitableeest, and composition of the present invention can protect banana to keep out mould at least 15 days.
Embodiment 6
Processing to banana
Experiment described herein is according to embodiment 4 described carrying out.But in this experiment, be respectively 21.3g/l and 1.0g/l with concentration
(DSM) and xanthans replace binding agent
And surfactant
At a bunch handle place, every bunch of 3 bunches of comprising 4 bananas are carried out twice damage, each bunch generation is 6 place's wounds altogether.
After hatching in 15 days, all wounds of two kinds of contrast banana groups all have fungus growth.Only with natamycin or phosphite handle bunch all wounds mould is also all arranged.And cross with the compositions-treated that comprises natamycin and phosphite bunch on, only observe fungus growth (seeing Table 6) at 1 place's wound.
The result of Zhan Shiing shows herein, but composition cover crop of the present invention (fruit for example, for example; banana and pineapple) avoid fungus growth; and show, than use separately the activity of reactive compound, composition of the present invention shows the collaborative activity that strengthens.
Table 1: the fungus growth in the banana wound
| Composition | Wound number/wound the sum that shows fungus growth * |
| Composition a (contrast 1) | 6/6 |
| Composition b (contrast 2) | 6/6 |
| Composition c (natamycin) | 6/6 |
| Composition d (phosphite) | 5/6 |
| Composition e (natamycin+phosphite) | 2/6 |
*Wound adds up to 6 (3 bananas, every banana 2 place's wounds)
Table 2: the fungus growth in the banana wound
| Composition | Wound number/wound the sum that shows fungus growth * |
| Composition a (contrast 1) | 3/3 |
| Composition b (contrast 2) | 3/3 |
| Composition c (natamycin) | 2/3 |
| Composition d (phosphite) | 3/3 |
| Composition e (natamycin+phosphite) | 1/3 |
*Wound adds up to 3 (3 bananas, every banana 1 place's wounds)
Table 3: the fungus growth in bunch on the banana
| Composition | Banana number/banana the sum that shows fungus growth * |
| Composition a (contrast 1) | 12/12 |
| Composition b (contrast 2) | 12/12 |
| Composition c (natamycin) | 12/12 |
| Composition d (phosphite) | 12/12 |
| Composition e (natamycin+phosphite) | 3/12 |
*Banana adds up to 12 (3 bunches, every bunch of 4 bananas)
Table 4: the serious fungus growth in bunch in the banana wound
| Composition | Number of clusters/bunch the sum that shows serious fungus growth * |
| Composition a (contrast 1) | 3/3 |
| Composition b (contrast 2) | 3/3 |
| Composition c (natamycin) | 3/3 |
| Composition d (phosphite) | 2/3 |
| Composition e (natamycin+phosphite) | 0/3 |
*Bunch add up to 3
Table 5: the fungus growth in the banana wound
| Composition | Wound number/wound the sum that shows fungus growth * |
| Composition a (contrast 1) | 12/12 |
| Composition b (contrast 2) | 12/12 |
| Composition c (natamycin) | 6/12 |
| Composition d (phosphite) | 3/12 |
| Composition e (natamycin+phosphite) | 0/12 |
*Wound adds up to 12 (6 bananas, every banana 2 place's wounds)
Table 6: the fungus growth in bunch in the banana wound
| Composition | Wound number/wound the sum that shows fungus growth * |
| Composition a (contrast 1) | 6/6 |
| Composition b (contrast 2) | 6/6 |
| Composition c (natamycin) | 6/6 |
| Composition d (phosphite) | 6/6 |
| Composition e (natamycin+phosphite) | 1/6 |
*Wound adds up to 6 (3 bunches, every bunch of 4 bananas are made 2 place's wounds at bunch handle place to every bunch)
List of references
Griffee PJ and Pinegar JA(1974)，Fungicides for control of the bananacrown rot complex：in vivo and in vitro studies.Trop.Sci.16，3：107-120.
Lapeyre de Bellaire de L and Dubois C (1987)，Distribution ofThiabendazole-Resistant Colletotrichum musae Isolates from GuadeloupeBanana Plantations Plant disease 81：1378-1383
Claims (12)
1. technology of handling fruit, described fruit are selected from the group that banana and pineapple constitute, and described technology comprises the steps: and will comprise polyene fungicide and at least a compound compositions that contains orthophosphite is applied to described fruit.
2. the technology of claim 1 is wherein handled described fruit after results.
3. the technology of claim 2, wherein, to described fruit since the wound that results cause handled.
4. the technology of claim 3, wherein, described wound causes owing to remove described fruit from plant.
5. any described technology in the claim 1 to 4, wherein, during described fruit is cleaned or use described composition afterwards.
6. any described technology in the claim 1 to 5, wherein, described polyene fungicide is a natamycin.
7. any described technology in the claim 1 to 6, wherein, described composition also comprises at least a added compound in the group that the compound that is selected from binding agent, surfactant, other antifungal compound and resists insect, nematode, acarid and/or bacterium constitutes.
8. any described technology in the claim 1 to 7, wherein, the application of described composition is used for pre-mould proofly growing on described fruit or fruit.
9. comprising polyene fungicide and at least a compound compositions that contains orthophosphite is used to protect the fruit that is selected from the group that banana and pineapple constitute to avoid the purposes of fungus growth.
10. fruit, it is selected from the group that banana and pineapple constitute, and through comprising polyene fungicide and at least a compound compositions that contains orthophosphite was handled.
11. the fruit of claim 7, wherein, with described composition handle described fruit because the wound that causes of results.
12. the fruit of claim 7 or 8, wherein, described composition also comprises at least a added compound in the group that the compound that is selected from binding agent, surfactant, other antifungal compound and resists insect, nematode, acarid and/or bacterium constitutes.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP06125673 | 2006-12-08 | ||
| EP06125673.1 | 2006-12-08 | ||
| EP07112772 | 2007-07-19 | ||
| EP07112772.4 | 2007-07-19 | ||
| PCT/EP2007/063420 WO2008068308A2 (en) | 2006-12-08 | 2007-12-06 | Post-harvest treatment of fruits with an antifungal composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101553122A true CN101553122A (en) | 2009-10-07 |
| CN101553122B CN101553122B (en) | 2013-07-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007800453743A Expired - Fee Related CN101553122B (en) | 2006-12-08 | 2007-12-06 | Post-harvest treatment of bananas with a new antifungal composition |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN101553122B (en) |
| ES (1) | ES2351772T3 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113040216B (en) * | 2021-04-02 | 2022-04-15 | 中国农业科学院农产品加工研究所 | Preparation method of antibacterial preservative paper, product and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4148891A (en) * | 1974-07-03 | 1979-04-10 | Gist-Brocades N.V. | Antifungal preparations |
| FR2732191B1 (en) * | 1995-03-30 | 2000-12-29 | Rhone Poulenc Agrochimie | ANTIFUNGAL TREATMENT OF BANANA TREES |
-
2007
- 2007-12-06 ES ES07847898T patent/ES2351772T3/en active Active
- 2007-12-06 CN CN2007800453743A patent/CN101553122B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101553122B (en) | 2013-07-10 |
| ES2351772T3 (en) | 2011-02-10 |
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