CN101548641A - Breeding method of high-quality protein maize hybrid - Google Patents

Abstract

The invention discloses a breeding method of high-quality protein maize hybrid, comprising the following steps: performing grain-by-grain selection by using 718 multiplied by Chinese series 051/02 during F1 period according to phenotypic character of panicle to select nontransparent maize seeds with opaque 2 genes and endosperm of powder amylum so as to sow seeds, inbreeding during F2 period, and continuously inbreeding 4-5 periods according to the same method to obtain QPM inbred line Y77; performing grain-by-grain selection by using M017 multiplied by Chinese series 023/02 during F1 period according to phenotypic character of panicle to select the nontransparent-stated maize seeds with opaque 2 genes and endosperm of powder amylum so as to sow seeds, inbreeding during F2 period, and continuously inbreeding 4-5 periods according to the same method to obtain QPM inbred line Y76; and hybridizing by using the inbred line Y77 as female parent and the inbred line Y76 as male parent. The maize bred by using the method is ratheripe and excellent in lodging resistance and disease resistance, and has high content of fine-quality protein and high yield.

Description

A kind of selection of high-quality protein maize hybrid

Technical field

The invention belongs to the genetic breeding technical field of agricultural crops, particularly a kind of selection of high-quality protein maize hybrid.

Background technology

High-quality protein maize (Quality Protein Maize) can be abbreviated as QPM.It is meant the corn kernel protein content more than 15%, the corn type of lysine content more than 4 ground.

As far back as 1914, people such as Osborne just found that the amino acid composition of maize seed protein is incomplete, lack lysine and tryptophan, thereby the biological value utilization are not high.But, never find effective means for a long time to how the corn protein quality to be improved by the genetic breeding approach.Up to 1994, people such as Mertz find first that by a large amount of Biochemical Research lysine content improves 70% than conventional corn in the homozygotic endosperm of recessive mutation gene Opaque-2 (being called for short O2), full seed lysine content exceeds 1 times, and tryptophane is high more than 1 times.Nelson etc. has found to have the fl2 gene of equivalent effect again subsequently.Henceforth, be that the corn breeding of target makes remarkable progress with the protein quality improvement.In the 60-70 age, the nearly all seeds company of the U.S. has all carried out the high-lysine corn breeding.Released first high-lysine corn crossbreed U～24 in 1969, its lysine content is higher 1 times than conventional corn, and protein is higher by 50% than conventional corn.Lysine and tryptophane improve about 80% than conventional corn in the full seed of high-quality protein maize.Primary Study shows that this corn is used for animal and fowl fodder, can increase substantially breeding performonce fo animals, improves feed efficiency, saves food, and increases farmers' income.

The breeding objective of high-quality protein maize is decided to be: grain protein content is more than 15%; Lysine content is 4%.More than; Endosperm hardness is more than 2 grades; Suitable with conventional corn output; Water content was normal when endosperm was ripe, was difficult for fragmentation, and 70% germination rate is arranged.

At present China's high-quality protein maize germ plasm resource is also deficient relatively, and has the high-quality protein maize hybrid problem that yields poorly.

Summary of the invention

At above-mentioned problems of the prior art and defective, the object of the present invention is to provide a kind of disease-resistant, high-quality, plant type compactness, output surpasses the selection of the high-quality protein maize hybrid of conventional corn.

In order to realize that the technical solution that the foregoing invention order is adopted is, a kind of selection of high-quality protein maize hybrid specifically comprises the following steps:

1) opaque 2 gene corn inbred line Y ₇₇Seed selection

User 718 * in be 051/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, can obtain good QPM inbred line Y ₇₇, do female parent.

2) opaque 2 gene corn inbred line Y ₇₆Seed selection

With M017 * in be 023/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, can obtain good QPM inbred line Y ₇₆, do male parent.

3) seed selection of high-quality protein maize

With opaque 2 gene corn inbred line Y ₇₇Make maternal and opaque 2 gene corn inbred line Y ₇₆Make male parent, hybridization.

Description of drawings

Fig. 1 is the flow process of the selection of high-quality protein maize hybrid of the present invention.

Specific embodiment that provides below in conjunction with accompanying drawing and inventor and regional field trial example are described in further detail the selection of high-quality protein maize hybrid of the present invention.

Embodiment

Fig. 1 has provided the flow process of the selection of high-quality protein maize hybrid of the present invention, specifically may further comprise the steps:

1) opaque 2 gene corn inbred line Y ₇₇Seed selection

User 718 * in be 051/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, can obtain good QPM inbred line Y ₇₇, do female parent.

Family 718 is to obtain by recurrent selection is carried out in Huang four group body morning inside through agricultural technology service center, Hu County; In be 051/02 to draw QPM inbred line from the seed selection of crop institute of the Chinese Academy of Agricultural Sciences, its be by generation early be 51～6/02 assorted select good strains in the field for seed to educate form.Y ₇₇Belong to semi-rigid opaque 2 seed types, 2.0 grades of hard degree, lysine content 0.47%, protein content 11.60%, every nutrient component is all apparently higher than conventional corn.

Y ₇₇The bud scale yellow green, blade is grayish green, leaf margin has ripple, be green, becomes strain plant height 145cm, fringe position 53cm, the number of blade 18, the leaf angle is little, the plant type compactness, dark green leaf color, tassel is medium, 3～4 of branch amounts, branch is isometric, and pollen amount is big, and flower pesticide is yellowish green, filigree is yellowish green, high resistant to lodging, fall folding, drought resisting, drought-enduring, Chinese People's Anti-Japanese Military and Political College's helminthosporium maydis, anti-head-smut, resistance to bacterial wilt, anti-rough dwarf disease.

Y ₇₇The fruit ear cylindricality, spike length 15cm, the thick 4.0cm of fringe, head progeny row 16～18, thousand kernel weight 210g, the fringe handle is long, bract is loose, cob white, fecundity is good, seed is yolk yellow, middle solids type partially belongs to the new inbred line of semi-rigid endosperm typical case's high-quality protein maize.

Y ₇₇Belong to precocious, plant type is compact, low, the big grain of fringe position spy type, sow in spring 110 days breeding times summer sowing 93 days.

2) opaque 2 gene corn inbred line Y ₇₆Seed selection

With M017 * in be 023/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, can obtain good QPM inbred line Y ₇₆, do male parent;

Inbred line Y ₇₆Seedling leaf sheath reddish violet, blade is grayish green, the seedling gesture is medium; Become strain plant height 167cm, the high 62cm in fringe position, the number of blade 19, blade is wide, internode is short, and blade is intensive, and tassel is medium, 3～4 of branch amounts, the tassel stipes is short, thick, crisp, and pollen amount is big, and flower pesticide is yellowish green, bract is long and thick, dual spike rate 30%, high resistant to lodging, fall folding, drought resisting, drought-enduring, anti-blue or green withered, anti-fringe Maize Ear Rot, leaf blight MR0.5, helminthosporium maydis A0.5, in ripe property good, it is long to hold the green phase, the one-tenth strain is sturdy.

Inbred line Y ₇₆The long taper of fruit ear, spike length 17～18cm, the thick 3.6cm of fringe, head progeny row 14～16, thousand kernel weight 183g, cob white, there are the point of doing, the little male inflorescence of heading tape sometimes in the fringe top.Yellow grain semihard endosperm, lysine content 0.42%, protein 11.55%, hard degree 3.5 belongs to the new inbred line of MAIZE WITH HARD ENDOSPERM high-quality protein maize, and the various qualities of this inbred line also all are better than conventional corn.

Y ₇₆Ripe long fringe in the genus, drought resisting type, the 118 days time of infertility, summer sowing 97 days.

3) seed selection of QPM

With opaque 2 gene corn inbred line Y ₇₇Make maternal and opaque 2 gene corn inbred line Y ₇₆Make male parent, hybridization, called after Shan list 17, former test code number Shan 9506;

1. single 17 breed standards in Shan

A, bud scale reddish violet, seedling is grayish green, and blade is more long narrow, seedling gesture stalwartness, become the pearl plant type loose, the number of blade 20, internode is long, and blade is sparse, plant height 258.0cm, the high 97.6cm in fringe position, the thick 3.5cm of stem, 4～5 of tassel branches, median size, flower pesticide yellow, filigree yellow green, long cartridge type fringe, spike length 19.5cm, tassel row number 18, row grain is several 36, thousand kernel weight 328.0g, the heavy 141.8g of single fringe grain, white axle, the yellow half horse tooth of seed, semi-rigid endosperm, 2.0～3.0 grades of hard degree;

B, this product kind belong to the precocious QPM of summer sowing, and this kind is first precocious QPM of summer sowing that participates in country's authorization, its grain test weight 730g/l, crude protein 11.53%, crude fat 5.61%, thick starch 67.83%, lysine 0.46%; The Comprehensive Assessment grain quality reaches national conventional corn one-level, feed corn one-level, edible corn one-level, high-quality protein maize secondary standard.According to Ministry of Agriculture's cereal and quality of item supervision and inspection center (Harbin) check, the single 17 grain test weight 738g/l in QPM Shan, crude protein 9.42%, crude fat 3.69%, starch 73.25%, lysine 0.41%, its nutritional quality are unique kinds that reaches national high-quality protein maize 2 grade standards in participating in the experiment kind.

4) producing method for seed:

Date of seeding: quadrature maternal breeding Y ₇₇* male parent Y ₇₆But, the sowing same period; Reciprocal cross production of hybrid seeds male parent Y ₇₆* maternal Y ₇₇, broadcast maternal Y earlier ₇₇, broadcast male parent Y again behind the apparent row of emerging ₇₆

Production of hybrid seeds row ratio: male parent: female parent is 1: 3 or 1: 4;

Seedling 4500 strains～5000 strains are stayed in production of hybrid seeds field.

5) the seed selection process in QPM Shan single 17

Participated in inside and outside spring sowing of institute and summer sowing multiple years product in 1997～1999 years and have outstanding performance than test, the production of hybrid seeds is quickened in winter in 1999 Hainan; Participated in the test of national special corn the Yellow River and Huai He River sea summer sowing A group in 2000～2002 years, and by this institute be organized in that Tunliu, Shanxi, Hebei are perfectly sound, Taiyuan, Shanxi, the high-quality special corn meeting of the whole nation, Chengdu, Sichuan are showed, test, in the Jingyang, Shaanxi, Baoji, Yang Ling area carries out multiple spot test and demonstration; Participated in the Summer Maize Production test of national the Yellow River and Huai He River sea in 2002, the Plant Protection Office of Hebei Academy of Agricultural Sciences is inoculated disease and insect resistance and is identified, in the San Nian district examination time limit, grain quality supervision and inspection center of the Ministry of Agriculture (Beijing) and Ministry of Agriculture's cereal and quality of item supervision and inspection center (Harbin) carry out that attributional analysis is consistent to be thought: Shan 9506 is high-quality protein maize new varieties that country district examination in the current year filters out unique secondary up to standard, under the guidance at the good numerous place of national agricultural technology spread service centre, Henan Province seed control station of this group hosting unit is consistent with the Hebei province seed control station thinks that this kind is that the precocious high-quality protein maize new varieties in summer sowing district, the Yellow River and Huai He River sea recommend country to authorize, and formally naming is QPM Shan single 17.

6) single 17 regional tests over the years in Shan, production demonstration, multiple spot result of experiment gather

Regional test: 2000 annual output 6883.5kg/hm ², comparison is according to tucking in single 19 underproduction 3.7%, and difference does not reach significantly, occupies second of test, in 19 pilots, has 11 places to increase production the underproduction of 8 places.Calendar year 2001 average yield 7273.5kg/hm ², comparison is according to agricultural university's 108 underproduction 10.8%, and difference is extremely remarkable, occupies the 4th of test, in 16 pilots, has 4 places to increase production the underproduction of 12 places.

Produce demonstration: 2002 annual production tests, average yield 6867.0kg/hm ², comparison occupies the tenth according to agricultural university's 108 average underproduction 13.74%, 2 volume increase in 26 pilots, and 24 underproduction are 6 equal underproduction in province.

Multiple years experiment: variety comparative test spring sowing 8250kg/hm in 1997～1999 years institutes ²～10050kg/hm ², than Yedan No.13 volume increase 3.2%～11.9%, summer sowing output 7125kg/hm ²～9000kg/hm ², than single No. 4 of family, single No. nine volume increase 5～15% in Shan.2000～calendar year 2001 is participated in the precocious group of Shaanxi Province's summer sowing regional test, 3 average yield 7980.0kg/hm in 2000 ², 2 some underproduction of volume increase than single No. 4 volume increase 9.2% in conventional corn contrast family, occupy the 6th of 13 tested variety; Calendar year 2001 3 average yield 6957.0kg/hm ², 2 some underproduction of volume increase, output and contrast family single No. 4 quite.Calendar year 2001 is participated in the spring sowing corn introduction test of agricultural college of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology tissue, average yield 8550.0kg/hm ², contrast single No. 4 volume increase 13.2% in family, occupy the 18th of 50 introduced variety; The test of summer sowing corn introduction, average yield 7927.5kg/hm ², contrast single No. 4 volume increase 13.6% in family, occupy the 15th of 63 tested variety.Participated in the spring sowing corn introduction test A group of agricultural college of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology tissue in 2002, average yield 8415kg/hm ², contrast single No. 4 volume increase 10.3% in family, occupy the 5th of 20 tested variety; The test of summer sowing corn introduction, average yield 7498.5kg/hm ², contrast the single No. 4 average volume increase 18.0% in family, occupy the 4th of 20 tested variety.The comprehensive disease resistance in high-quality protein maize Shan single 17, prematurity obviously is better than list 9409 in the kind of the same type, than precocious 6 days of agricultural university 108, high-quality protein Shan 9506 is that unique one of district's examination in the current year reaches the conventional corn one-level, feed corn one-level, the edible corn one-level, the special corn of high-quality protein maize secondary newly makes up, it is the typical high-quality protein maize new varieties of the Yellow River and Huai He River Hai Xiayumiqu, has submitted country's authorization to.

7) adapt to area, yield level

Be suitable for the Yellow River and Huai He River Hai Xiaboqu such as Shaanxi, Jiangsu, Anhui, Henan, Tianjin, Shaanxi and North China spring sowing district.General summer sowing output 6750kg/hm ²～9000kg/hm ², the high-yield field can reach 10500kg/hm ²General spring sowing output 7500kg/hm ²～10500kg/hm ², the high-yield field can reach 11250kg/hm ², be that the feeding and grain of desirable seed is raised dual-purpose high-quality protein maize new varieties.

8) culture technique main points

Commercialization high-quality protein maize order is produced, and requires to concentrate in flakes, isolates relatively, guarantees quality.Summer sowing density is generally per hectare 49500 strains～56250 strains, 49500 strains of spring sowing per hectare.Use sufficient base fertilizer, heavy dressing jointing fertilizer, many organic fertilizers, N, P, K compounding application are effective.If later stage is chance wet weather, in time results, airing, anti-going mouldy.

9) seed type, ripe phase and crossbreed adaptability

Shaanxi and the Yellow River and Huai He River Hai Xiayumiqu, frost-free season is short, and early spring drought lies prostrate great drought, and cooling after autumn is the key constraints during the corn growth soon, the single 17 (Y in QPM Shan ₇₇* Y ₇₆) belong to (semihard * semihard) type, precocity, filling speed is fast, and is early ripe, adaptability is good, imported the tropical and subtropical zone germplasm, the bar maturation of living, it is good to protect green property, the seed commodity is good, the grass yield height is the first-class raw material of high-lysine, high protein of the Yellow River and Huai He River Hai Xiayumiqu exploitation green feed, and this kind does not have enterprise to develop as yet at present.

Table 1～district's test manufacture amount the result of table 6 country

Table 12001 year national corn variety production test Quality Detection result

Preside over unit: national agricultural technology spread service centre tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

Analyze Shan 9506QPM corn kernel unit weight 730g/L, crude protein 11.53%, crude fat 5.61%, thick starch 67.83%, lysine 0.46% according to grain quality supervision and inspection center of the Ministry of Agriculture (Beijing); The Comprehensive Assessment grain quality reaches national conventional corn one-level, feed corn one-level, edible corn one-level, high-quality protein maize secondary standard.According to Ministry of Agriculture's cereal and quality of item supervision and inspection center (Harbin) check, Shan 9506 grain test weight 738g/L, crude protein 9.42%, crude fat 3.69%, starch 73.25%, lysine 0.41%, its nutritional quality are unique kinds that reaches national high-quality protein maize 2 grade standards in participating in the experiment kind.

The special corn the Yellow River and Huai He River sea summer sowing A of table 2:2000 country group district's test manufacture amount result

Recommend unit: good Fan Chu Shaanxi Province seed control station of national agricultural technology spread service centre

Kind of test: national special corn the Yellow River and Huai He River sea summer corn A group

Unit is presided in test: the Henan Province seed control station

Tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

Agricultural University Of He'nan

Annotate: (1) CK is that conventional corn is tucked in list 19, occupies first,

(2) this group hosting unit is Henan Province seed control station and Agricultural University Of He'nan

According to single 17, the 2000 annual output 6883.5kg/hm in the QPM Shan of method seed selection of the present invention ², comparison is according to tucking in single 19 underproduction 3.7%, and difference does not reach significantly, occupies second of test, in 19 pilots, has 11 places to increase production the underproduction of 8 places.Calendar year 2001 average yield 7273.5kg/hm ², comparison is according to agricultural university's 108 underproduction 10.8%, and difference is extremely remarkable, occupies the 4th of test, in 16 pilots, has 4 places to increase production the underproduction of 12 places.The intersite coefficient of variation 24.8, yield stability is general, in the performance of ground such as Handan, Hebei, Jingyang, Shaanxi better.This kind plant type hemicompact is gathered, plant height 258.0cm, the high 97.6cm in fringe position, lodging rate 10.6%, fecundity is good, empty stalk rate 3.0%, bud scale reddish violet, 2 grades of helminthosporium maydis and stem rot, leaf blight, Curvularia lunata leaf spot, corn borer are caused harm and are 1 grade, black powder of light sense and fringe Maize Ear Rot.Spike length 19.5cm, tassel row number 15.6, row grain is several 34.9, the long 1.7cm of bald point, thousand kernel weight 328.0g, single fringe grain weight 141.8g, seed yellow, semihard grain type.This kind between breeding time 95.6 days, comparison is according to precocious, and suggestion is carried out production test in conjunction with the attributional analysis result in suitable zone.

Table 32001 year national special corn the Yellow River and Huai He River sea summer corn A group district's test manufacture amount result

Recommend unit: good Fan Chu Shaanxi Province seed control station of national agricultural technology spread service centre

The classification of participating in the experiment: national corn regional test the Yellow River and Huai He River sea A group

Unit is presided in test: the Henan Province seed control station

Tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

Agricultural University Of He'nan

Annotate: (1) CK is a conventional corn agricultural university 108

(2) this group hosting unit is Henan Province seed control station and Agricultural University Of He'nan

Table 42002 year national corn regional test the Yellow River and Huai He River sea summer corn group production test yield result

Recommend unit: good Fan Chu Shaanxi Province seed control station of national agricultural technology spread service centre

Kind of test: national corn regional test the Yellow River and Huai He River sea summer corn group production test

Preside over unit: Hebei province seed control station tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

National corn regional test the Yellow River and Huai He River sea summer corn group production demonstration test in 2002 is presided over by the Hebei province seed control station, and conclusion (of pressure testing) is as follows:

QPM Shan single 17: provided by academy of agricultural sciences, Shaanxi Province corn, be QPM.2002 annual production tests, average yield 6867.0kg/hm ², comparison occupies the tenth according to agricultural university's 108 average underproduction 13.74%, 2 volume increase in 26 pilots, and 24 underproduction are 6 equal underproduction in province.Participated in the Yellow River and Huai He River Hai Tezhongyumiqu examination in 2000, average yield 6883.5kg/hm ², comparison is according to tucking in single 19 underproduction 3.7%, and is not remarkable, has 11 to increase 8 and subtract in 19 pilots.Calendar year 2001 is participated in the Yellow River and Huai He River Hai Tezhongyumiqu examination, average yield 7273.5kg/hm ², comparison is according to agricultural university 108 underproduction 10.8%, and difference is extremely remarkable, has 4 to increase 12 and subtract in 16 pilots.

This kind plant type hemicompact is gathered, and plant height, fringe position are moderate, and prematurity is good.According to grain quality supervision and inspection center of the Ministry of Agriculture in 2002 to the grain quality analysis result.Beijing is surveyed: unit weight 730g/l, and crude protein 11.53%, crude fat 5.61%, thick starch 67.83%, lysine 0.46% is the highest duplicate samples of lysine in all test kinds; Harbin is surveyed: unit weight 738g/l, and crude protein 9.24%, crude fat 3.69%, thick starch 73.25%, lysine 0.41% is the highest duplicate samples of lysine in all test kinds.

Table 5 Shaanxi Province summer sowing corn (precocious group) regional test yield result

Preside over unit: the Shaanxi Province seed control station

Tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

The Baoji seed control station

Annotate: (1) contrast is Shaanxi summer sowing main breed, single No. 4 of conventional corn family

(2) every is all repeated unified variety comparative test for three times and puts down in writing by standard

Table 6 Shaanxi Province multiple spot test yield result

Preside over unit: agricultural college of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology

Tested variety: high-quality protein Shan 9506 (QPM Shan single 17)

Annotate: (1) contrast is single No. 4 of conventional corn family

(2) implement the place: Shaanxi Yang Ling Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology experimental plot

Variety comparative test spring sowing 8250～10050kg/hm in 1997～1999 years institutes ², than Yedan No.13 volume increase 3.2～11.9%, summer sowing output 7125～9000kg/hm ², than single No. 4 of family, single No. nine volume increase 5～15% in Shan.2000～calendar year 2001 is participated in the precocious group of Shaanxi Province's summer sowing regional test, 3 average yield 7980.0kg/hm in 2000 ², 2 some underproduction of volume increase than single No. 4 volume increase 9.2% in conventional corn contrast family, occupy the 6th of 13 tested variety; Calendar year 2001 3 average yield 6957.0kg/hm ², 2 some underproduction of volume increase, output and contrast family single No. 4 quite.Calendar year 2001 is participated in the spring sowing corn introduction test of agricultural college of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology tissue, average yield 8550.0kg/hm ², contrast single No. 4 volume increase 13.2% in family, occupy the 18th of 50 introduced variety; The test of summer sowing corn introduction, average yield 7927.5kg/hm ², contrast single No. 4 volume increase 13.6% in family, occupy the 15th of 63 tested variety.Participated in the spring sowing corn introduction test A group of agricultural college of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology tissue in 2002, average yield 8415kg/hm ², contrast single No. 4 volume increase 10.3% in family, occupy the 5th of 20 tested variety; The test of summer sowing corn introduction, average yield 7498.5kg/hm ², contrast the single No. 4 average volume increase 18.0% in family, occupy the 4th of 20 tested variety.The comprehensive disease resistance in QPM Shan 9506 (QPM Shan single 17), prematurity obviously is better than list 9409 in the kind of the same type, than precocious 6 days of agricultural university 108, high-quality protein Shan 9506 (QPM Shan single 17) is the unique conventional corn one-level that reaches of district's examination in the current year, feed corn one-level, the edible corn one-level, the special corn of high-quality protein maize secondary newly makes up, it is the typical high-quality protein maize new varieties of the Yellow River and Huai He River Hai Xiayumiqu, by country's authorization.

Claims (1)

1, a kind of selection of high-quality protein maize hybrid is characterized in that: specifically comprise the following steps:

1) opaque 2 gene corn inbred line Y ₇₇Seed selection

User 718 * in be 051/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, obtains good QPM inbred line Y ₇₇

2) opaque 2 gene corn inbred line Y ₇₆Seed selection

With M017 * in be 023/02, at F ₁In generation,, choosing endosperm was opaque starch according to the phenotypic character grain-by-grain seed selection of fringe portion, and seed is the corn kernel with opaque 2 genes of opaque state to be sowed, at F ₂In generation, carried out selfing, again 4～5 generations of selfing continuously after the same method, obtains good QPM inbred line Y ₇₆

3) seed selection of high-quality protein maize

With opaque 2 gene corn inbred line Y ₇₇Make maternal and opaque 2 gene corn inbred line Y ₇₆Make male parent, hybridization.

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