CN101545900B - Biological detector for quickly detecting mercuric compound - Google Patents
Biological detector for quickly detecting mercuric compound Download PDFInfo
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- CN101545900B CN101545900B CN200910084423A CN200910084423A CN101545900B CN 101545900 B CN101545900 B CN 101545900B CN 200910084423 A CN200910084423 A CN 200910084423A CN 200910084423 A CN200910084423 A CN 200910084423A CN 101545900 B CN101545900 B CN 101545900B
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- reaction tank
- test paper
- display screen
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Abstract
The invention discloses a biological detector for quickly detecting a mercuric compound, and relates to quick biological detection for mercuric pollution. The appearance of the detector is wedge-shaped; the upper surface of the detector is provided with a mercuric compound test paper port and a display screen; the interior of the detector is provided with a temperature control module arranged at the front end of a reaction tank, the reaction tank vertical to the test paper port and close to the temperature control module, a spot area measuring module parallel to the reaction tank, and a data processing module parallel to the spot area measuring module in turn from the wedge-shaped high end to the bottom end; and by processing, the concentration of the mercuric compound in a sample is finally read on the display screen. During analysis, only about 2 milliliters of sample solution is added into the reaction tank of the detector, a piece of mercuric compound biological test paper is inserted into the reaction tank, and the display screen of the detector reads the concentration of the sample mercuric compound after several minutes. The detector has the characteristics of sensitivity, high specificity, low cost and convenient use, and can quickly detect organic mercuric compounds such as mercury ions, methyl mercury, ethyl mercury and the like in an environmental sample.
Description
Technical field
The invention belongs to the analytical and testing instrument field, particularly the quick bio detector of mercury pollution.
Background technology
Mercury is a kind of important metallic element that nature exists, because it has multiple excellent characteristic, mercury and compound thereof are used in industrial and agricultural production very extensively.But mercury and compound thereof are again one type of endocrine disruptors, and chronic mercury exposes harm to human body and mainly shows and cause central nervous system disorder, injury of kidney, amentia even death.Detection to the environmental sample mercury ion at present mainly realizes (W.L.Clevenger through methods such as atomic absorption spectrum, atomic fluorescence spectrophotometry, inductivity coupled plasma mass spectrometries; B.W.Smith J.D.Winefordner Trace determination ofmercury:Areview.Crit.Rev.Anal.Chem.; 1997,27:1～26).Though these methods have very high sensitivity and accuracy; But need expensive large-scale instrument, long analytical cycle, complicated sample pre-treatment process; Also than higher, these all make it be difficult to use in the field quick detection of environmental sample to operating personnel's requirement.For solving this difficult problem, the biochemical analysis detection technique becomes the focus that people pay close attention to, and the biochemical analysis technology is mainly utilized the specificity of some bioactivator and mercury compound to interact and realized the check and analysis to mercury ion.At present, the mercury compound biochemistry detection technology that is seen in report can be divided into four types, i.e. method of analyzing enzyme, immune analysis method, specific proteins biology sensor, bioluminescent assay etc.Corresponding bioactivator is respectively (A.Amine such as enzyme, antibody, specific proteins, transgenic cell or microorganism; C.Cremisini; G.Palleschi.Determination of mercury (II); Methylmercuryand ethylmercury in the ng/mL range with an electrochemical enzyme glucose probe.Mikrochim.Acta, 1995,121:183-190).But these detection methods mainly are confined to the laboratory at present, during test sample, need to the pollution source sampling, and the laboratory pre-treatment detects again, can not provide testing result fast at the pollution source scene.
Summary of the invention
The object of the present invention is to provide a kind of easy sensitivity, mercury pollution bio-detector with low cost, can directly be used for environmental sample, like the field quick detection and the analysis of the mercury compound content of water body, soil, animal etc.
This instrument comprises following part: mercury compound detects test paper 1, display screen 2, and temperature control module 3, reaction tank 4, speck area is measured module 5, data processing module 6 etc.This detector outward appearance is wedge, and upper surface has mercury compound to detect test paper socket and display screen.The inside of detector is followed successively by to the bottom from wedge is high-end, temperature control module 3, and it is contained in the reaction tank front end, regulates control reaction tank temperature.Perpendicular to detecting the test paper socket, being close to temperature control module is reaction tank 4, and testing sample solution is equipped with in the inside, and test strip 1 is inserted reaction tank 4 and testing sample reaction.With reaction tank 4 parallel positions speck area is housed and measures module 5, a digital camera mainly is equipped with in its inside, is used for the area of shot detection test paper reaction back colour developing spot.With speck area measure module 5 parallel be data processing module 6, the speck area that the spot analyzer is taken is transferred to data processing module 6, through handling, finally on display screen 2, reads mercury compound concentration in the sample.Only need during the mercury compound check and analysis about 2 ml sample solution are joined in the reaction tank of instrument, insert mercury compound biological detection bar, the concentration that can read the sample mercury compound after a few minutes by the instrument display screen.
The characteristics of this detector be sensitive, specificity is high, with low cost and easy to use, organomercury compounds such as the mercury ion in can the fast detecting environmental sample, methyl mercury, ethyl mercury.
Description of drawings
Fig. 1 is a mercury compound quick bio detector external structure synoptic diagram, and wherein 1 for mercury compound detects test paper, and 2 is display screen.
Fig. 2 is a detector inner structure synoptic diagram, and wherein 1 for mercury compound detects test paper, and 2 is display screen, and 3 is temperature control module, and 4 is reaction tank, and 5 are speck area mensuration module, and 6 is data processing module.
Embodiment
Fig. 1 is a mercury compound quick bio detector external structure synoptic diagram, and Fig. 2 is a detector inner structure synoptic diagram, comprises like the lower part: mercury compound detects test paper, display screen, and temperature control module, reaction tank, speck area is measured module, data processing module etc.During analytic sample, needing it is carried out simple pre-treatment, is soil sample like sample, with its dissolving, removes and does not dissolve the impurity part, gets its mercury compound crude extract; If living animal like fish, is directly got its blood, dilute or be added drop-wise on the detector bar and get final product; If water sample solution, the dilution or directly detect.Getting the testing sample solution that 2mL handled well joins in the reaction tank; Insert mercury compound biological detection test paper; Detecting test paper is made up of polyester membrane carrier, reaction zone two parts; Reaction zone comprises that a slice is fixed with the CAM of urase and the accurate pH test paper that a slice has been adsorbed urea and trehalose, can see blue color spot point at the edge of pH test paper after 2 minutes, the detection test paper of fixed position is taken pictures by preset parameter with digital camera; Utilize image processing software, the area of computing center's spot.Because speck area size in center becomes positive correlation with mercury compound concentration, through calculating, optimize the Flame Image Process parameter to speck area on a large amount of test strips, the instrument display screen gets final product the concentration of show sample mercury compound after 8 minutes.
Claims (1)
1. the bio-detector of a fast detecting mercury compound is characterized in that, comprises that mercury compound detects test paper, display screen, temperature control module, reaction tank, speck area mensuration module, data processing module; Outward appearance is wedge; Upper surface has mercury compound to detect test paper socket and display screen, and the inside of detector is from the high-end temperature control module that is contained in the reaction tank front end that is followed successively by to the bottom of wedge, perpendicular to detecting the test paper socket and being close to the reaction tank of temperature control module; The speck area parallel with reaction tank measured module; Measure the parallel data processing module of module with speck area,, finally on display screen, read mercury compound concentration in the sample through handling; Detect test paper and be made up of polyester membrane carrier, reaction zone two parts, reaction zone comprises that a slice is fixed with the CAM of urase and the accurate pH test paper that a slice has been adsorbed urea and trehalose, and speck area is measured inside modules a digital camera is housed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910084423A CN101545900B (en) | 2009-05-15 | 2009-05-15 | Biological detector for quickly detecting mercuric compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910084423A CN101545900B (en) | 2009-05-15 | 2009-05-15 | Biological detector for quickly detecting mercuric compound |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101545900A CN101545900A (en) | 2009-09-30 |
| CN101545900B true CN101545900B (en) | 2012-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910084423A Expired - Fee Related CN101545900B (en) | 2009-05-15 | 2009-05-15 | Biological detector for quickly detecting mercuric compound |
Country Status (1)
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| CN (1) | CN101545900B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109541149B (en) * | 2018-12-29 | 2023-06-06 | 广东工业大学 | Biological type atmospheric heavy metal pollution detection and discrimination device and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322947A (en) * | 2001-06-05 | 2001-11-21 | 中国科学院生态环境研究中心 | Mecury compound testing strip based on enzyme inhibiting reaction |
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2009
- 2009-05-15 CN CN200910084423A patent/CN101545900B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322947A (en) * | 2001-06-05 | 2001-11-21 | 中国科学院生态环境研究中心 | Mecury compound testing strip based on enzyme inhibiting reaction |
Non-Patent Citations (2)
| Title |
|---|
| 时国庆等.汞化合物的生物检测技术.《环境污染治理技术与设备》.2004,第5卷(第6期),6-11. * |
| 谢少涛等.原子荧光光谱法测定水中微量汞.《仪器仪表与分析监测》.1999,(第2期),54-56. * |
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| CN101545900A (en) | 2009-09-30 |
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