CN101538292A - Separation and purification technology of trehalose by one-step method - Google Patents
Separation and purification technology of trehalose by one-step method Download PDFInfo
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- CN101538292A CN101538292A CN200910103610A CN200910103610A CN101538292A CN 101538292 A CN101538292 A CN 101538292A CN 200910103610 A CN200910103610 A CN 200910103610A CN 200910103610 A CN200910103610 A CN 200910103610A CN 101538292 A CN101538292 A CN 101538292A
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- trehalose
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Abstract
The invention relates to an extraction, separation and purification method of trehalose, in particular to a separation and purification method of trehalose by one-step. The method comprises the steps of: after extracting dry yeast by using water, adding a flocculating agent to adjust the pH value; and then filtering to obtain clear filtrate, and directly concentrating and crystallizing to obtain trehalose crystals. The method has simple separation and purification steps, convenience and feasibility, low cost and good effect, and provides conditions for the trehalose to be widely used in the fields of medicine, cosmetics, food preservation and the like.
Description
Technical field
The present invention relates to separation and purification trehalose technology from dry yeast.
Background technology
Have the trehalose of title of " sugar of life " nontoxic, human body is had no side effect, essential property is similar to other disaccharide.But himself has non-specific provide protection; can make organism behind the drying and dehydrating with extremely low and even stop metabolic form it is protected; in case environmental permission; organism can be brought back to life; but do not decrease living matter, so trehalose is widely used in aspects such as the drying preservation, medical science, health, beauty treatment of food.The interior method of producing trehalose of world wide mainly contains enzyme transforming process and extracts from barms at present.Domestic a company has adopted enzyme transforming process to produce trehalose, but it is subjected to starting material---the restriction of tapioca (flour) plantation; And traditional yeast extraction method purification procedures is many, causes production cost height, low, the valuable product of target product, has seriously limited the widespread use of trehalose.
Summary of the invention
The objective of the invention is to remedy the prior art above shortcomings, propose the technology of single stage method separation and purification trehalose, extraction process is simple, and is effective.
Technical scheme of the present invention is as follows:
The technology of single stage method separation and purification trehalose, utilize solvent under room temperature-80 ℃ from dry yeast by stirring by solid and liquid mass volume ratio 1: 100-1: 1 (g: mL) extract trehalose, add vitriol by extracting the amount that adds 5 grams among the aqueous trehalose 100ml then, by regulating the pH value to neutral, generate flocculation agent, filter promptly to get then and clarify aqueous trehalose, condensing crystal can get product.
Described solvent comprises the ethanolic soln of various concentration.
Can directly filter with flame filter press after forming flocculation agent, flocculation agent can directly get clear filtrate as filtration medium.
Regulate pH and can use all alkaloids.
The present invention can reduce purification procedures, only can reach the purifying purpose with i.e. filtration of a step, greatly reduces production cost, for the widespread use of trehalose provides condition.
Description of drawings
Fig. 1 adopts the white powder trehalose crystal pattern that obtains after the inventive method crystallization.
Embodiment
Below describe implementation process of the present invention and beyond thought many-sided positive effect of being brought in detail by specific embodiment; be intended to help the reader to understand better and understand essence of the present invention and the novelty place; but but practical range of the present invention is not constituted any qualification; in the instruction of this explanation with any modification and the change made under inspiring, all should belong to the protection domain of claims of the present invention for the personage who knows art technology to technical solution of the present invention.
Embodiment 1.
Get 80% the ethanolic soln that the 5g dry yeast adds 100ml, in 80 ℃ of scopes, stir and extracted 2 hours.Extracting solution is added 3g zinc sulfate,, filter with flame filter press to neutral with the sodium hydroxide adjust pH, the gained clear filtrate is the good aqueous trehalose of purifying.
Embodiment 2.
Get the water that the 5g dry yeast adds 100ml, in 40 ℃ of scopes, stir and extracted 2 hours.Extracting solution is added 5g zinc sulfate,, filter with flame filter press to neutral with the calcium oxide adjust pH, the gained clear filtrate is the good aqueous trehalose of purifying.
Embodiment 3.
Get 50% ethanolic soln that the 10g dry yeast adds 100ml, in 50 ℃ of scopes, stir and extracted 2 hours.Extracting solution is added 4g zinc sulfate,, filter with flame filter press to neutral with the sodium hydroxide adjust pH, the gained clear filtrate is the good aqueous trehalose of purifying.
Embodiment 4.
Get 70% ethanolic soln that the 3g dry yeast adds 100ml, in 60 ℃ of scopes, stir and extracted 2 hours.Extracting solution is added 6g zinc sulfate,, filter with flame filter press to neutral with the sodium hydroxide adjust pH, the gained clear filtrate is the good aqueous trehalose of purifying.
Embodiment 5.
Get 70% ethanolic soln that the 3g dry yeast adds 100ml, in 70 ℃ of scopes, stir and extracted 2 hours.Extracting solution is added 6g zinc sulfate,, filter with flame filter press to neutral with the sodium hydroxide adjust pH, the gained clear filtrate is the good aqueous trehalose of purifying.
Extract trehalose by aforesaid method, its extraction yield on average can reach 15g trehalose/100g dry yeast.Can obtain white powder trehalose crystal after the proper method crystallization, as shown in Figure 1.
Claims (3)
1, the technology of single stage method separation and purification trehalose, it is characterized in that: utilize solvent under room temperature-80 ℃, from dry yeast, to extract trehalose by stirring, the mass volume ratio of dry yeast and solvent is 1: 100-1: 1, the amount that adds 5 grams then among the aqueous trehalose 100ml by said extracted adds zinc sulfate, by regulating the pH value to neutral, generate flocculation agent, filter promptly to get then and clarify aqueous trehalose, condensing crystal gets product;
Described solvent is the ethanolic soln of various concentration.
2, the technology of single stage method separation and purification trehalose according to claim 1 is characterized in that: directly filter with flame filter press after forming flocculation agent, flocculation agent directly gets clear filtrate as filtration medium.
3, the technology of single stage method separation and purification trehalose according to claim 1 and 2 is characterized in that transferring pH to adopt all alkaloids.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101036103A CN101538292B (en) | 2009-04-15 | 2009-04-15 | Separation and purification technology of trehalose by one-step method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101036103A CN101538292B (en) | 2009-04-15 | 2009-04-15 | Separation and purification technology of trehalose by one-step method |
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| Publication Number | Publication Date |
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| CN101538292A true CN101538292A (en) | 2009-09-23 |
| CN101538292B CN101538292B (en) | 2011-06-08 |
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| CN2009101036103A Expired - Fee Related CN101538292B (en) | 2009-04-15 | 2009-04-15 | Separation and purification technology of trehalose by one-step method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102924539A (en) * | 2012-10-31 | 2013-02-13 | 南京工业大学 | Method for preparing trehalose crystals |
| CN105218597A (en) * | 2011-09-21 | 2016-01-06 | 株式会社林原 | The preparation method of the powder containing α, α-trehalose dihydrate solvate crystal |
-
2009
- 2009-04-15 CN CN2009101036103A patent/CN101538292B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218597A (en) * | 2011-09-21 | 2016-01-06 | 株式会社林原 | The preparation method of the powder containing α, α-trehalose dihydrate solvate crystal |
| US9657045B2 (en) | 2011-09-21 | 2017-05-23 | Hayashibara Co., Ltd. | Process for producing a particulate composition comprising crystalline trehalose dihydrate |
| US9738674B2 (en) | 2011-09-21 | 2017-08-22 | Hayashibara Co., Ltd. | Process for producing a particulate composition comprising crystalline trehalose dihydrate |
| CN105218597B (en) * | 2011-09-21 | 2017-10-10 | 株式会社林原 | Contain α, the preparation method of the powder of alpha trehalose dihydrochloride dihydrate crystal |
| CN107446007A (en) * | 2011-09-21 | 2017-12-08 | 株式会社林原 | Contain α, the preparation method of the powder of alpha trehalose dihydrochloride dihydrate crystal |
| CN107602631A (en) * | 2011-09-21 | 2018-01-19 | 株式会社林原 | Contain α, the preparation method of the powder of alpha trehalose dihydrochloride dihydrate crystal |
| US9938311B2 (en) | 2011-09-21 | 2018-04-10 | Hayashibara Co., Ltd. | Process for producing a particulate composition comprising crystalline alpha, alpha-trehalose di-hydrate |
| US10196416B2 (en) | 2011-09-21 | 2019-02-05 | Hayashibara Co., Ltd. | Process for producing a particulate composition comprising crystalline alpha, alpha-trehalose di-hydrate |
| US10301342B2 (en) | 2011-09-21 | 2019-05-28 | Hayashibara Co., Ltd. | Process for producing a particulate composition comprising crystalline α,α-trehalose di-hydrate |
| CN102924539A (en) * | 2012-10-31 | 2013-02-13 | 南京工业大学 | Method for preparing trehalose crystals |
| CN102924539B (en) * | 2012-10-31 | 2015-05-20 | 南京工业大学 | Method for preparing trehalose crystals |
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| CN101538292B (en) | 2011-06-08 |
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Granted publication date: 20110608 Termination date: 20120415 |