CN101536807A - Continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method and device thereof - Google Patents
Continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method and device thereof Download PDFInfo
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- CN101536807A CN101536807A CN200910083317A CN200910083317A CN101536807A CN 101536807 A CN101536807 A CN 101536807A CN 200910083317 A CN200910083317 A CN 200910083317A CN 200910083317 A CN200910083317 A CN 200910083317A CN 101536807 A CN101536807 A CN 101536807A
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Abstract
The invention discloses a continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method and a device thereof, and belongs to the field of liquid food process. The sterilising and enzyme inactivating device in the invention mainly comprises a sterilising and enzyme inactivating tube (18), a gas spiral pre-heater (10), a liquid material spiral pre-heater (15) and a pressure buffer tank (27). When the pressure of the sterilising and enzyme inactivating tube (18) is 7.5-60MPa, a pressure-relief micro-regulating valve (25) and a one-way valve (36) at the outlet of the sterilising and enzyme inactivating tube are regulated, and a material blow valve (37) is slowly opened such that the pressure in the sterilising and enzyme inactivating tube (18) is a constant specified value between 7.5MPa and 60MPa, and finally, the liquid material is connected in a material connecting tank (29). The invention can realize continuous operation and treatment, and has the characteristics that the invention is simply and conveniently operated, safe and highly effective. Under the same treatment conditions, the sterilising and enzyme inactivating effect is greatly better than a static reactor.
Description
Technical field
The present invention relates to a kind of sterilising and enzyme inactivating method and device thereof, particularly a kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method and device thereof.
Background technology
The food liquid sterilising and enzyme inactivating is handled and is adopted prior heat treatment mostly, so the loss of the thermal sensitivity active material in the food liquid is more, and the food liquid after the heat treatment has the boiling flavor; The ultra-high pressure sterilization of Chu Xianing carries out at a lower temperature after this, solved the problem of heat-sensitive substance degraded to a certain extent, but owing to technology, equipment characteristic, cost is higher, and be confined to static pressurize processing mode, the extensive high efficiency suitability for industrialized production of difficult realization.
The high-pressure carbon dioxide sterilising and enzyme inactivating technology use high-pressure carbon dioxide can be under relatively low temperature sterilising and enzyme inactivating, the high-pressure carbon dioxide bactericidal unit that research is at present used mainly is the sterilizer based on the static reaction still, though static sterilization still has the advantage of handling liquid and solid-state two states product, but treatment effeciency is relatively low, and it is difficult to be used for industrial mass production.Static state or dynamic process mode directly influence the efficient of high-pressure carbon dioxide sterilising and enzyme inactivating.High-pressure carbon dioxide is used for the processing of food liquid, and continous way dynamic machining equipment is only and meets standard and the requirement that large-scale industrialization is produced efficiently.
Summary of the invention
The object of the invention is to provide a kind of device of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating.
Another object of the present invention is to provide and use said apparatus to carry out the method for sterilising and enzyme inactivating.
A kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating device comprises high-pressure carbon dioxide generation and induction system, liquid material induction system, microvesicle distributor 11, sterilising and enzyme inactivating pipe 18, sample collecting system.Described high-pressure carbon dioxide takes place and induction system is that carbon dioxide steel cylinder 1 is connected with refrigeration compressor 2 and molecular sieve filter 3 successively through first check valve 30 and second check valve 31, molecular sieve filter 3 outlets are connected with carbon dioxide storage tank 6 inlets, carbon dioxide storage tank 6 outlets connect gases at high pressure pump 7 inlets through the 3rd check valve 32,7 outlets of gases at high pressure pump link to each other with gas spiral preheater 10 inlets, 10 outlets of gas spiral preheater link to each other with the inlet of microvesicle distributor 11 through the 4th check valve 33,11 outlets of microvesicle distributor are connected to sterilising and enzyme inactivating pipe 18 bottom inlets, and mass-flow gas meter 8 is installed in gases at high pressure pump 7 porch;
Described liquid material induction system is that 12 outlets of liquids batch can link to each other with pressure liquid pump 13, pressure liquid pump 13 outlets link to each other with liquid material spiral preheater 15 inlets, 15 outlets of liquid material spiral preheater link to each other with the outlet of microvesicle distributor 11 upper ends through the 5th check valve 34, and liquid material mass flowmenter 14 is installed in pressure liquid pump 13 porch;
Described sample collecting system is that 18 outlets of sterilising and enzyme inactivating pipe are connected to pressure surge tank 27 through sterilising and enzyme inactivating pipe outlet check valve 36 and decompression reset valve 25, and pressure surge tank 27 outlets connect material atmospheric valve 37, and material atmospheric valve 37 lower ends are material collection jar 29.
Described microvesicle distributor 11 is evenly distributed to high-pressure carbon dioxide gas in the flowing liquid material.
Described gas spiral preheater 10 can carry out preheating to the highly pressurised liquid material that enters sterilising and enzyme inactivating pipe 18.
Described liquid material spiral preheater 15 can carry out preheating to the high-pressure carbon dioxide gas that enters sterilising and enzyme inactivating pipe 18.
Described sterilising and enzyme inactivating pipe 18 tops adopt polytetrafluoroethylene (PTFE) assembly 20 to carry out radial seal.
Described sterilising and enzyme inactivating pipe 18 tops are connected with second Pressure gauge 19, inner the 4th temperature control thermocouple 21, the 5th temperature control thermocouple 22, the 6th temperature control thermocouple 23 and the 7th temperature control thermocouple 24 that temperature in the real-time monitoring pipe is arranged from top to bottom.
The outer wall of described sterilising and enzyme inactivating pipe 18 is provided with electric heating layer 17, and the top is connected with safety anti-explosive device 38.
Described safety anti-explosive device 38 can increase the security of whole sterilising and enzyme inactivating device, and the effect of pressure in the unloading sterilising and enzyme inactivating pipe 18 is arranged.
Described pressure surge tank 27 outer walls have a circulator bath heater 28, guarantee that liquid material evenly flows out continuously, solve the stifled problem of ice.
A kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method, operate according to following steps:
1), preheating sterilising and enzyme inactivating pipe 18, gas spiral preheater 10 and liquid material spiral preheater 15 and pressure surge tank 27, wherein, gas spiral preheater 10 is identical with the temperature that liquid material spiral preheater 15 and sterilising and enzyme inactivating pipe 18 are set, be 35-65 ℃, pressure surge tank 27 outer water bath heating temperatures are 35-40 ℃;
2), close sterilising and enzyme inactivating pipe outlet check valve 36, the frequency of setting pressure liquid pump 13 and gases at high pressure pump 7 is 5-50Hz, liquid material is pumped into sterilising and enzyme inactivating pipe 18 with the flow velocity of 0.5-10kg/h after 15 heating of liquid material spiral preheater, carbon dioxide pumps into sterilising and enzyme inactivating pipe 18 through gas spiral preheater 10 and microvesicle distributor 11 successively with the flow velocity of 0.5-5kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe 18 is 7.5-60MPa, regulate decompression reset valve 25 and sterilising and enzyme inactivating pipe outlet check valve 36, and slowly open material atmospheric valve 37, to keep the particular value of constant pressure between 7.5-60MPa in the sterilising and enzyme inactivating pipe 18;
3), collect liquid material in material collection jar 29.
The mass ratio of described liquid material and carbon dioxide mix is 2: 1-10: 1.
The frequency decision liquid material flow velocity and the processing time of described pressure liquid pump 13.
The described liquid material processing time is the flow velocity of the volume/liquid material of sterilising and enzyme inactivating pipe.
The frequency of described gases at high pressure pump 7 is set according to liquid material flow velocity, processing pressure and temperature.
Advantage of the present invention: this continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating device adopts integrated design, has easy and simple to handle and advantage such as experimental safe.Wherein the performance of this sterilising and enzyme inactivating reaction unit has greatly been optimized in the employing of microvesicle distributor 11, safety anti-explosive device 38, decompression reset valve 25 and material collection jar 29, and highlights its good safety advantages, has strengthened the sterilising and enzyme inactivating effect.Experimental results show that in the Juice that with the carbon dioxide the is pressure medium enzyme effect of going out to be better than the method for prior heat treatment, the gas burst size does not constitute pollution to environment, and in addition, the present invention is applicable to the food liquid sterilising and enzyme inactivating that is rich in the thermal sensitivity active material.
Description of drawings
Fig. 1 is the structural representation of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating device of the present invention.
Wherein, the 1-carbon dioxide steel cylinder, the 2-refrigeration compressor, the 3-molecular sieve filter, 4-first Pressure gauge, the 5-first temperature control thermocouple, 6-carbon dioxide storage tank, 7-gases at high pressure pump, the 8-mass-flow gas meter, the 9-second temperature control thermocouple, 10-gas spiral preheater, 11-microvesicle distributor, 12-liquids batch can, 13-pressure liquid pump, 14-liquid material mass flowmenter, 15-liquid material spiral preheater, 16-the 3rd temperature control thermocouple, 17-electric heating layer, 18-sterilising and enzyme inactivating pipe, 19-second Pressure gauge, 20-polytetrafluoroethylene (PTFE) assembly, 21-the 4th temperature control thermocouple, 22-the 5th temperature control thermocouple, 23-the 6th temperature control thermocouple, 24-the 7th temperature control thermocouple, the 25-reset valve that reduces pressure, 26-the 8th temperature control thermocouple, 27-pressure surge tank, 28-circulator bath heater, 29-material collection jar, 30-first check valve, 31-second check valve, 32-the 3rd check valve, 33-the 4th check valve, 34-the 5th check valve, 35-the 6th check valve, 36-sterilising and enzyme inactivating pipe outlet check valve, 37-material atmospheric valve, 38-safety anti-explosive device.
The specific embodiment
Following examples only are used to the present invention is described and unrestricted the present invention.
The used gases at high pressure pump 7 of the present invention is a duplex plunger pump, and maximum stream flow is 5kg/h, and flow can be regulated in the 0.5-5kg/h scope by adjusting two plunger positions; Pressure liquid pump 13 is two duplex plunger pumps in parallel, and maximum stream flow is 10kg/h or 2kg/h, and can use one by change-over switch, and wherein maximum stream flow is that the pressure liquid pump 13 of 2kg/h can be regulated in the 0.5-2kg/h scope by adjusting two plunger positions; Microvesicle distributor 11 is a cylindrical exterior, and internal diameter is 4cm, and height is 5cm, and three layers of stainless (steel) wire sheet are arranged in the microvesicle distributor 11, and its aperture is the 400-1200 order; Mass-flow gas meter 8 and liquid material mass flowmenter 14 are Coriolis mass flowmeter; Sterilising and enzyme inactivating pipe 18 is cylindrical, and sterilising and enzyme inactivating pipe 18 volumes among the present invention are that (3.5i.d. * 1500cm), maximum tolerable pressure is 60MPa to 1442ml.
Embodiment 1
As shown in Figure 1, a kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating device, carbon dioxide steel cylinder 1 links to each other with refrigeration compressor 2 with second check valve 31 through first check valve 30, refrigeration compressor 2 outlets connect molecular sieve filter 3, molecular sieve filter 3 is connected to carbon dioxide storage tank 6, carbon dioxide is compressed through refrigeration compressor 2, and after molecular sieve filter 3 filtered, being in a liquid state was stored in the carbon dioxide storage tank 6.Be connected with first Pressure gauge 4 and the first temperature control thermocouple 5 on the carbon dioxide storage tank 6.Carbon dioxide storage tank 6 is connected to gases at high pressure pump 7 through the 3rd check valve 32,7 outlets of gases at high pressure pump insert gas spiral preheater 10, liquid carbon dioxide pumps through gases at high pressure pump 7, enters gas spiral preheater 10, is connected to microvesicle distributor 11 through the 4th check valve 33; Liquids batch can 12 links to each other with pressure liquid pump 13, pressure liquid pump 13 outlets link to each other with liquid spiral preheater 15,15 outlets of liquid spiral preheater link to each other with microvesicle distributor 11 outlet upper ends through the 5th check valve 34, converge with 11 outlets of microvesicle distributor to be connected to sterilising and enzyme inactivating pipe 18 bottom inlets.Mass-flow gas meter 8 and liquid material mass flowmenter 14 are installed in gases at high pressure pump 7 and pressure liquid pump 13 porch respectively, monitoring carbon dioxide and liquid material flow velocity.Carbon dioxide evenly disperses to enter in the liquid material through microvesicle distributor 11.The temperature of sterilising and enzyme inactivating pipe 18 is evenly monitored by the 4th temperature control thermocouple 21, the 5th temperature control thermocouple 22, the 6th temperature control thermocouple 23, the 7th temperature control thermocouple 24, pressure is shown by second Pressure gauge 19, sterilising and enzyme inactivating pipe 18 tops adopt polytetrafluoroethylene (PTFE) assembly 20 to carry out radial seal, outer wall is provided with electric heating layer 17, and the top is connected with safety anti-explosive device 38.18 outlets of sterilising and enzyme inactivating pipe are through sterilising and enzyme inactivating pipe outlet check valve 36, decompression reset valve 25 connects pressure surge tank 27, pressure surge tank 27 lower ends connect material atmospheric valve 37, pressure surge tank 27 outer walls have a circulator bath heater 28, the sterilising and enzyme inactivating pipe 18 liquid mixture material of working off one's feeling vent one's spleen enters pressure surge tank 27, and its temperature is by 26 monitoring of the 8th temperature control thermocouple.Handle the back liquid charging stock and enter material collection jar 29 through 37 discharges of material atmospheric valve from pressure surge tank 27 outlets.
Embodiment 2-5 is the experiment of peroxidase and polyphenol oxidase in the employing continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method passivation red beet extract
Red beet extract raw material is prepared: with the red beet chopping, mix in 1: 2.5 ratio of mass ratio with deionized water, stirred 1 minute, place to extract after 30 minutes under the room temperature in stirrer for mixing, filter through four layer of 200 order filter cloth, filter liquor is stand-by as the red beet extract.
Embodiment 2
1), preheating sterilising and enzyme inactivating pipe 18, gas spiral preheater 10 and liquid material spiral preheater 15 and pressure surge tank 27, wherein, the temperature that gas spiral preheater 10, liquid material spiral preheater 15 and sterilising and enzyme inactivating pipe 18 are set is 55 ℃, and pressure surge tank 27 outer water bath heating temperatures are 40 ℃;
2), close sterilising and enzyme inactivating pipe outlet check valve 36, the frequency of setting pressure liquid pump 13 and gases at high pressure pump 7 is respectively 10Hz, the red beet extract is pumped into sterilising and enzyme inactivating pipe 18 with the flow velocity of 2.88kg/h (processing time is 30.04min) after 15 heating of liquid material spiral preheater, carbon dioxide pumps into sterilising and enzyme inactivating pipe 18 through gas spiral preheater 10 and microvesicle distributor 11 successively with the flow velocity of 1.20kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe 18 is 7.5MPa, regulate decompression reset valve 25 and sterilising and enzyme inactivating pipe outlet check valve 36, and slowly open material atmospheric valve 37, to keep constant pressure in the sterilising and enzyme inactivating pipe 18 at 7.5MPa;
3), collect liquid material in material collection jar 29.
The enzymatic activity of red beet extract is measured after adopting AAS to above-mentioned processing, the remaining vigor of gained peroxidase (POD) is 23.14%, the remaining vigor of polyphenol oxidase (PPO) is 7.12% (POD in the untreated control sample, the enzyme activity of PPO is in 100%).The enzyme processing time that goes out is 30min, compare with static high pressure carbon dioxide, prior heat treatment (no carbon dioxide), effect is comparatively desirable, and (uniform pressure, temperature and processing time are after the static high pressure carbon dioxide treatment, the remaining vigor of POD is 53.07%, and the remaining vigor of PPO is 14.09%; The same treatment temperature and time, the remaining vigor of POD is 82.74% after the prior heat treatment, the remaining vigor of PPO is 59.95%).
Embodiment 3
1), preheating sterilising and enzyme inactivating pipe 18, gas spiral preheater 10 and liquid material spiral preheater 15 and pressure surge tank 27, wherein, the temperature that gas spiral preheater 10, liquid material spiral preheater 15 and sterilising and enzyme inactivating pipe 18 are set is 50 ℃, and pressure surge tank 27 outer water bath heating temperatures are 37 ℃.
2), close sterilising and enzyme inactivating pipe outlet check valve 36, the frequency of setting pressure liquid pump 13 and gases at high pressure pump 7 is 10Hz, the red beet extract is pumped into sterilising and enzyme inactivating pipe 18 with the flow velocity of 2.88kg/h (processing time is 30.04min) after 15 heating of liquid material spiral preheater, carbon dioxide pumps into sterilising and enzyme inactivating pipe 18 through gas spiral preheater 10 and microvesicle distributor 11 successively with the flow velocity of 1.20kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe 18 is 15MPa, regulate decompression reset valve 25 and sterilising and enzyme inactivating pipe outlet check valve 36, and slowly open material atmospheric valve 37, to keep constant pressure in the sterilising and enzyme inactivating pipe 18 at 15MPa;
3), collect liquid material in material collection jar 29.
Enzymatic activity after adopting spectrophotometry to handle in the sample, the remaining vigor of more than handling in the gained red beet extract of peroxidase (POD) is 25.93%, the remaining vigor of polyphenol oxidase (PPO) is 7.63% (POD in the untreated control sample, the enzyme activity of PPO is in 100%).The enzyme processing time that goes out is 30min, compare with static high pressure carbon dioxide, prior heat treatment (no carbon dioxide), effect is comparatively desirable, and (uniform pressure, temperature and processing time are after the static high pressure carbon dioxide treatment, the remaining vigor of POD is 70.32%, and the remaining vigor of PPO is 35.45%; The same treatment temperature and time, the remaining vigor of POD is 92.78% after the prior heat treatment, the remaining vigor of PPO is 78.99%).
Embodiment 4
1), preheating sterilising and enzyme inactivating pipe 18, gas spiral preheater 10 and liquid material spiral preheater 15 and pressure surge tank 27, wherein, the temperature that gas spiral preheater 10, liquid material spiral preheater 15 and sterilising and enzyme inactivating pipe 18 are set is 35 ℃, and pressure surge tank 27 outer water bath heating temperatures are 35 ℃.
2), close sterilising and enzyme inactivating pipe outlet check valve 36, the frequency of setting pressure liquid pump 13 and gases at high pressure pump 7 is 10Hz, the red beet extract is pumped into sterilising and enzyme inactivating pipe 18 with the flow velocity of 2.88kg/h (processing time is 30.04min) after 15 heating of liquid material spiral preheater, carbon dioxide pumps into sterilising and enzyme inactivating pipe 18 through gas spiral preheater 10 and microvesicle distributor 11 successively with the flow velocity of 1.20kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe 18 is 7.5MPa, regulate decompression reset valve 25 and sterilising and enzyme inactivating pipe outlet check valve 36, and slowly open material atmospheric valve 37, to keep constant pressure in the sterilising and enzyme inactivating pipe 18 at 7.5MPa;
3), collect liquid material in material collection jar 29.
The enzymatic activity of red beet extract is measured after adopting AAS to above-mentioned processing, the remaining vigor of gained peroxidase (POD) is 79.54%, the remaining vigor of polyphenol oxidase (PPO) is 47.36% (POD in the untreated control sample, the enzyme activity of PPO is in 100%).The enzyme processing time that goes out is 30min, compares effect comparatively desirable (same treatment temperature and time, the remaining vigor of POD is 100.39% after the prior heat treatment, the remaining vigor of PPO is 88.00%) with prior heat treatment (no carbon dioxide).
Embodiment 5
1), preheating sterilising and enzyme inactivating pipe 18, gas spiral preheater 10 and liquid material spiral preheater 15 and pressure surge tank 27, wherein, the temperature that gas spiral preheater 10, liquid material spiral preheater 15 and sterilising and enzyme inactivating pipe 18 are set is 65 ℃, and pressure surge tank 27 outer water bath heating temperatures are 40 ℃.
2), close sterilising and enzyme inactivating pipe outlet check valve 36, readjustment pressure liquid pump and the total range of gases at high pressure pump are original half, the frequency of setting pressure liquid pump 13 and gases at high pressure pump 7 is 10Hz, the red beet extract is pumped into sterilising and enzyme inactivating pipe 18 with the flow velocity of 1.44kg/h (processing time is 15.02min) after 15 heating of liquid material spiral preheater, carbon dioxide pumps into sterilising and enzyme inactivating pipe 18 through gas spiral preheater 10 and microvesicle distributor 11 successively with the flow velocity of 0.6kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe 18 is 7.5MPa, regulate decompression reset valve 25 and sterilising and enzyme inactivating pipe outlet check valve 36, and slowly open material atmospheric valve 37, to keep constant pressure in the sterilising and enzyme inactivating pipe 18 at 7.5MPa;
3), collect liquid material in material collection jar 29.
The enzymatic activity of red beet extract is measured after adopting AAS to above-mentioned processing, the remaining vigor of gained peroxidase (POD) is 20.83%, the remaining vigor of polyphenol oxidase (PPO) is 5.15% (POD in the untreated control sample, the enzyme activity of PPO is in 100%).The enzyme processing time that goes out is 15min, compares effect comparatively desirable (same treatment temperature and time, the remaining vigor of POD is 80.33% after the prior heat treatment, the remaining vigor of PPO is 58.67%) with prior heat treatment (no carbon dioxide).
Claims (8)
1, a kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating device, comprise that high-pressure carbon dioxide takes place and induction system, the liquid material induction system, microvesicle distributor (11), sterilising and enzyme inactivating pipe (18), sample collecting system, it is characterized in that, described high-pressure carbon dioxide takes place and induction system is that carbon dioxide steel cylinder (1) is connected with refrigeration compressor (2) and molecular sieve filter (3) successively through first check valve (30) and second check valve (31), molecular sieve filter (3) outlet is connected with carbon dioxide storage tank (6) inlet, carbon dioxide storage tank (6) outlet connects gases at high pressure pump (7) inlet through the 3rd check valve (32), gases at high pressure pump (7) outlet links to each other with gas spiral preheater (10) inlet, gas spiral preheater (10) outlet links to each other through the inlet of the 4th check valve (33) with microvesicle distributor (11), microvesicle distributor (11) outlet is connected to sterilising and enzyme inactivating pipe (18) bottom inlet, and mass-flow gas meter (8) is installed in gases at high pressure pump (7) porch;
Described liquid material induction system is that liquids batch can (12) outlet links to each other with pressure liquid pump (13), pressure liquid pump (13) outlet links to each other with liquid material spiral preheater (15) inlet, liquid material spiral preheater (15) outlet links to each other with the outlet of microvesicle distributor (11) upper end through the 5th check valve (34), and liquid material mass flowmenter (14) is installed in pressure liquid pump (13) porch;
Described sample collecting system is that sterilising and enzyme inactivating pipe (18) outlet is connected to pressure surge tank (27) through sterilising and enzyme inactivating pipe outlet check valve (36) and decompression reset valve (25), pressure surge tank (27) outlet connects material atmospheric valve (37), and material atmospheric valve (37) lower end is material collection jar (29).
2, sterilising and enzyme inactivating device according to claim 1 is characterized in that, described microvesicle distributor (11) is evenly distributed to high-pressure carbon dioxide gas in the flowing liquid material.
3, sterilising and enzyme inactivating device according to claim 1 is characterized in that, described sterilising and enzyme inactivating pipe (18) top adopts polytetrafluoroethylene (PTFE) assembly (20) to carry out radial seal.
4, sterilising and enzyme inactivating device according to claim 1, it is characterized in that, described sterilising and enzyme inactivating pipe (18) top is connected with second Pressure gauge (19), inner the 4th temperature control thermocouple (21), the 5th temperature control thermocouple (22), the 6th temperature control thermocouple (23) and the 7th temperature control thermocouple (24) that temperature in the real-time monitoring pipe is arranged from top to bottom.
5, sterilising and enzyme inactivating device according to claim 1 is characterized in that, the outer wall of described sterilising and enzyme inactivating pipe (18) is provided with electric heating layer (17), and the top is connected with safety anti-explosive device (38).
6, sterilising and enzyme inactivating device according to claim 1 is characterized in that, described pressure surge tank (27) outer wall has a circulator bath heater (28).
7, a kind of continuous dynamic high-pressure carbon dioxide sterilising and enzyme inactivating method is characterized in that, comprises following operating procedure:
1), preheating sterilising and enzyme inactivating pipe (18), gas spiral preheater (10) and liquid material spiral preheater (15) and pressure surge tank (27), wherein, gas spiral preheater (10) is identical with the temperature that liquid material spiral preheater (15) and sterilising and enzyme inactivating pipe (18) are set, be 35-65 ℃, the outer water bath heating temperature of pressure surge tank (27) is 35-40 ℃;
2), close sterilising and enzyme inactivating pipe outlet check valve (36), the frequency of setting pressure liquid pump (13) and gases at high pressure pump (7) is 5-50Hz, liquid material is pumped into sterilising and enzyme inactivating pipe (18) with the flow velocity of 0.5-10kg/h after liquid material spiral preheater (15) heating, carbon dioxide pumps into sterilising and enzyme inactivating pipe (18) through gas spiral preheater (10) and microvesicle distributor (11) successively with the flow velocity of 0.5-5kg/h simultaneously, when the pressure of sterilising and enzyme inactivating pipe (18) is 7.5-60MPa, regulate decompression reset valve (25) and sterilising and enzyme inactivating pipe outlet check valve (36), and slowly open material atmospheric valve (37), to keep the particular value of constant pressure between 7.5-60MPa in the sterilising and enzyme inactivating pipe (18);
3), collect liquid material in material collection jar (29).
8, sterilising and enzyme inactivating method according to claim 8 is characterized in that, the mass ratio of described liquid material and carbon dioxide mix is 2: 1-10: 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102113697A (en) * | 2010-11-19 | 2011-07-06 | 广东海洋大学 | High-density carbon dioxide sterilizing device and refreshing method for shrimps |
| CN102283418A (en) * | 2011-06-03 | 2011-12-21 | 张守勤 | Ultrahigh-cold isostatic pressing continuous sterilization method and device for liquid food |
| CN102583817A (en) * | 2012-02-02 | 2012-07-18 | 南京大学 | Printing and dyeing tail water deep treatment and reuse process |
| CN101703787B (en) * | 2009-11-19 | 2012-07-25 | 中国农业大学 | Method for continuously performing operations of sterilizing, seeping and pressure releasing on liquid material under super-high pressure |
| CN108967960A (en) * | 2018-10-11 | 2018-12-11 | 沈阳农业大学 | A kind of preparation method of sea-buckthorn whole fruit freeze-dried powder |
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2009
- 2009-04-29 CN CN2009100833175A patent/CN101536807B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703787B (en) * | 2009-11-19 | 2012-07-25 | 中国农业大学 | Method for continuously performing operations of sterilizing, seeping and pressure releasing on liquid material under super-high pressure |
| CN102113697A (en) * | 2010-11-19 | 2011-07-06 | 广东海洋大学 | High-density carbon dioxide sterilizing device and refreshing method for shrimps |
| CN102283418A (en) * | 2011-06-03 | 2011-12-21 | 张守勤 | Ultrahigh-cold isostatic pressing continuous sterilization method and device for liquid food |
| CN102583817A (en) * | 2012-02-02 | 2012-07-18 | 南京大学 | Printing and dyeing tail water deep treatment and reuse process |
| CN102583817B (en) * | 2012-02-02 | 2014-06-18 | 南京大学 | Printing and dyeing tail water deep treatment and reuse process |
| CN108967960A (en) * | 2018-10-11 | 2018-12-11 | 沈阳农业大学 | A kind of preparation method of sea-buckthorn whole fruit freeze-dried powder |
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| Publication number | Publication date |
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| CN101536807B (en) | 2011-05-04 |
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