CN101530817A - Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure - Google Patents
Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure Download PDFInfo
- Publication number
- CN101530817A CN101530817A CN200810034505A CN200810034505A CN101530817A CN 101530817 A CN101530817 A CN 101530817A CN 200810034505 A CN200810034505 A CN 200810034505A CN 200810034505 A CN200810034505 A CN 200810034505A CN 101530817 A CN101530817 A CN 101530817A
- Authority
- CN
- China
- Prior art keywords
- air
- negative pressure
- minus
- filter
- pathogen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title abstract description 8
- 238000002474 experimental method Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 5
- 238000004737 colorimetric analysis Methods 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 abstract description 21
- 244000052769 pathogen Species 0.000 abstract description 18
- 239000000443 aerosol Substances 0.000 abstract description 6
- 238000002955 isolation Methods 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000000149 penetrating effect Effects 0.000 abstract 3
- 230000002498 deadly effect Effects 0.000 abstract 2
- 238000011045 prefiltration Methods 0.000 abstract 2
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Landscapes
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Filtering Of Dispersed Particles In Gases (AREA)
Abstract
The invention relates to a negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure. The method is realized through the following steps: the downstream part of a primarily efficient air pre-filter is caused to form differential pressure that is between minus 5-minus 10 Pa with external air at an air inlet; after penetrating through an intermediately efficient air pre-filter, air stream forms the differential pressure between minus 80-minus 100 Pa with the external air; the differential pressure of the air pressures in a biological operation experiment chamber, a return air grille and a pipeline is at least kept at minus 200 Pa; the differential pressure of the air stream penetrating through a first stage efflux superhigh efficiency air filter reaches minus 280-minus 300 Pa; and the differential pressure of the air stream penetrating through a second stage efflux superhigh efficiency air filter reaches minus 360-minus 400 Pa. The negative pressure method has the beneficial effects that when a deadly pathogen medium is operated, pathogen aerosol is absolutely isolated and does not cause the risk that people are affected with the deadly pathogen; and even if the cabinet body has a tiny cavity similar to a needle point, the negative pressure technology can also ensure the absolute isolation of the pathogen.
Description
Technical field
The present invention relates to a kind of Biohazard Safety Equipment, relate in particular to the negative pressure of this Biohazard Safety Equipment.
Background technology
Biohazard Safety Equipment is an air-flow enveloping field, and the air intake air-flow is used for the protection of operating space to experiment sample behind high-efficiency air filtering; The inflow air-flow of action pane forms the isolation air curtain inside and outside the operating space, exempts from pathogenic infection with the protection personnel and threatens; The air-flow that effluxes enters atmosphere or the laboratory is in order to prevent the pathogen aerosol infection through high-efficiency air filtering.
The uprush that the two stage biological safety cabinet is used for the operating space being protected by experiment sample is the coupling by blower fan and high efficiency particle air filter, and plenum chamber formation air positive pressure is also realized through high efficiency particle air filter.When this designing technique is applied to circulating current, consider that the filter efficiency of air cleaner is 99.99%, then ten thousand/quantity is mingled in the vertical down current in the pathogen aerosol, makes sample infect and has inevitable property.Even in the B2 of no circulating current class Biohazard Safety Equipment, because the opening of operation window exists, front window inflow air-flow produces any breach and all will cause the pathogen aerosol to escape.Therefore, the Biohazard Safety Equipment of this biological isolation Level is not possess isolating power completely for the operation of fatal pathogen.
For fully satisfying unknown pathogen or exogenous pathogen and sample being taken precautions against the special operational needs that pollution has complete isolating power occasion, a kind of biological isolation technology that runs on fully under the negative pressure state is the problem that the technical staff will solve.
Summary of the invention
The technical issues that need to address of the present invention have provided a kind of negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully, are intended to solve the above problems.
In order to solve the problems of the technologies described above, the present invention realizes by following steps:
Make at the air intake place and just to imitate the air pre cleaner downstream and promptly form pressure reduction with extraneous air, pressure reduction-5 ~-10Pa between;
Air-flow see through behind the middle effect air pre cleaner with the pressure reduction of extraneous air-80Pa ~-100Pa Negative Pressure Difference scope in;
The air pressure of biological inside, operation experiments cabin and return air grid and pipeline maintains-the 200Pa Negative Pressure Difference at least;
See through the air-flow differential that the first order effluxes the super grade high efficiency air cleaner to reach-280Pa ~-the 300Pa range of negative pressure in;
See through the air-flow differential that the second level effluxes the super grade high efficiency air cleaner to reach-360Pa ~-the 400Pa range of negative pressure in.
Compared with prior art, the invention has the beneficial effects as follows: when fatal pathogen medium is operated, the pathogen aerosol will definitely be isolated and not the formation personnel face the risk of fatal pathogenic infection; Even the cavity of minimum similar needle point appears in cabinet, negative pressure technique can guarantee that also pathogen is definitely isolated.
Description of drawings
Fig. 1 adopts three-grade biological safety cabinet front view of the present invention;
Fig. 2 is the side view of Fig. 1;
Fig. 3 is the vertical view of Fig. 1;
The specific embodiment
Below in conjunction with the accompanying drawing and the specific embodiment the present invention is described in further detail:
The present invention realizes by following steps:
1, make at the air intake place and just imitate the air pre cleaner downstream and promptly form pressure reduction with extraneous air, pressure reduction-5 ~-10Pa between;
2, air-flow see through behind the middle effect air pre cleaner with the pressure reduction of extraneous air-80Pa ~-100Pa;
3, the air pressure of biological inside, operation experiments cabin and return air grid and pipeline maintains-200Pa pressure reduction at least;
4, see through the air-flow differential that the first order effluxes the super grade high efficiency air cleaner to reach-280Pa ~-300Pa;
5, see through the air-flow differential that the second level effluxes the super grade high efficiency air cleaner to reach-360Pa ~-400Pa;
Wherein in step " 1 ": the specification of filter is that relative 〉=1.0 μ m particulate filtration efficient are greater than 70%;
Wherein in step " 2 ": the specification of filter is colorimetric method filter efficiency 〉=90%;
Wherein in step " 3 ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%;
Wherein in step " 4 ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%;
Wherein in step " 5 ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%.
The present invention utilizes air-flow to see through filter and produces the principle that air pressure falls, make and just imitate the air pre cleaner downstream and promptly form pressure reduction with extraneous air, air-flow is through darker with the pressure reduction of extraneous air behind the middle effect air pre cleaner, the air pressure of biological inside, operation experiments cabin and return air grid and pipeline maintains-the 200Pa level at least, see through the air-flow differential that the first order effluxes the super grade high efficiency air cleaner to reach-280Pa ~-300Pa, see through the air-flow differential that the second level effluxes the super grade high efficiency air cleaner to reach-360Pa ~-400Pa.
In the biological operation experiments cabin and keep a degree of depth pressure reduction between the external world, be the basic means that the present invention realizes biological isolation, when fatal pathogen medium is operated, the pathogen aerosol will definitely be isolated and not the formation personnel face the risk of fatal pathogenic infection; Even the cavity of minimum similar needle point appears in cabinet, negative pressure technique can guarantee that also pathogen is definitely isolated.
By Fig. 1, Fig. 2, Fig. 3 visible (mark is with the structure of the step design adopted among the present invention among the figure):
The three-grade biological safety cabinet that runs on negative pressure state fully is an airtight box-shaped operation cabinet.The cabinet exterior panel all adopts similar stainless steel material machine-shaping with parts such as biological operation experiments cabin inner side wall plate, biological pass-through boxes, continuous inferior arc welding process is all adopted in connection between them, and the cabinet Sealing Technology can stably satisfy+and 500Pa air pressure keeps behind the 30min still the airtight requirement greater than+450Pa overbottom pressure down.
By cabinet, just imitate that air pre cleaner, middle effect air pre cleaner, air intake plenum chamber and super grade high efficiency air cleaner, biological operation experiments cabin, return air grid and pipeline, two-stage efflux the super grade high efficiency air cleaner, can set up blast regulator, terminal air draft blower fan is formed system.
Originally exempt from pollution for protection is subjected to sample, vertical down current has following guarantee in the biological operation experiments cabin:
A. vertical down current filters through ULPA feilter, and the air-flow that operation is provided is aseptic dustless purifying gas flow.
B. the internal gas flow flow velocity evenly distributes, and wallboard piston sample made the inner All Ranges in operating space be the effective coverage by the whole operation district around down current was fitted.
C. no longer there is delta-shaped region in air-flow unidirectional motion fully vertically downward in the biological operation experiments cabin, and the airless vortex has suppressed the generation that internal chiasma is infected fully.Under operating personnel's arm motion condition, the generation that can effectively avoid internal chiasma to infect equally.
D. upwards to the vertical height of vertical ventilation filter or homogenizing plate, the cross section air-flow mean flow rate of differing heights equates biological operation experiments cabin table top.
Adopt the device of control system air flow rate of the present invention, this device is set and the maintenance air flow rate.It and blower fan control system interlock are carried out dual control to air flow rate, after blower fan control system changes, can set up blast regulator and still can stably control flow, so that system keeps negative pressure design load and vertical down current mean flow velocity value.
The terminal blower fan is born the device of system's air-supply and air draft, and design is in the terminal of exhaust airduct.The terminal blower fan has enough static pressure pulling airflow passes whole systems, is the power that system runs on negative pressure state fully.
The closed system that runs on negative pressure state fully is to stop the absolute assurance means that pathogen is leaked, and needs only not dead electricity of system, just can keep the isolation pathogen ability of its brilliance.
Claims (6)
1. negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully, realize by following steps:
(1), make at the air intake place and just to imitate the air pre cleaner downstream and promptly form pressure reduction with extraneous air, pressure reduction-5 ~-10Pa between;
(2), air-flow see through behind the middle effect air pre cleaner with the pressure reduction of extraneous air-80Pa ~-100Pa;
(3), the air pressure of biological inside, operation experiments cabin and return air grid and pipeline maintains-200Pa pressure reduction at least;
(4), seeing through air-flow differential that the first order effluxes the super grade high efficiency air cleaner reaches-280 ~-300Pa pressure reduction;
(5), seeing through air-flow differential that the second level effluxes the super grade high efficiency air cleaner reaches-360 ~-400Pa pressure reduction.
2. the negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully according to claim 1, wherein in step " (1) ": the specification of filter is that relative 〉=1.0 μ m particulate filtration efficient are greater than 70%.
3. the negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully according to claim 1, wherein in step " (2) ": the specification of filter is colorimetric method filter efficiency 〉=90%.
4. the negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully according to claim 1, wherein in step " (3) ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%.
5. the negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully according to claim 1, wherein in step " (4) ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%.
6. the negative pressure that runs on the three-grade biological safety cabinet of negative pressure state fully according to claim 1, wherein in step " (5) ": the specification of filter is that relative 0.1 μ m particulate filtration efficient is greater than 99.9995%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810034505A CN101530817A (en) | 2008-03-12 | 2008-03-12 | Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810034505A CN101530817A (en) | 2008-03-12 | 2008-03-12 | Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101530817A true CN101530817A (en) | 2009-09-16 |
Family
ID=41101802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810034505A Pending CN101530817A (en) | 2008-03-12 | 2008-03-12 | Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101530817A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172447A (en) * | 2011-03-14 | 2011-09-07 | 中南大学 | Method for treating experimental waste liquid in secondary biological safety cabinet and device thereof |
| CN102814202A (en) * | 2011-06-10 | 2012-12-12 | 北京世安科兴科技开发有限责任公司 | III level biological safety cabinet |
-
2008
- 2008-03-12 CN CN200810034505A patent/CN101530817A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172447A (en) * | 2011-03-14 | 2011-09-07 | 中南大学 | Method for treating experimental waste liquid in secondary biological safety cabinet and device thereof |
| CN102814202A (en) * | 2011-06-10 | 2012-12-12 | 北京世安科兴科技开发有限责任公司 | III level biological safety cabinet |
| CN102814202B (en) * | 2011-06-10 | 2015-09-23 | 北京世安科兴科技开发有限责任公司 | A kind of III level biological safety cabinet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101264726B (en) | Filtration purification overpressure and negative pressure integrated protection system for vehicle | |
| CN204100393U (en) | The new blower fan of way flow Two-way Cycle | |
| CN103372361A (en) | Single pulse bag type dust collector | |
| CN101530817A (en) | Negative pressure method of a class III biosafety cabinet operating fully under the condition of negative pressure | |
| CN107008080A (en) | A kind of cartridge-type filter cylinder dust-collecting equipment | |
| CN102814202B (en) | A kind of III level biological safety cabinet | |
| CN107192081A (en) | A kind of fresh air-changing device and fully-automatic intelligent air regenerating device | |
| CN201558343U (en) | Disinfection type biological safety cabinet | |
| CN105920933B (en) | High-efficient energy-saving environment friendly dust arrester | |
| CN210251725U (en) | High-sealing aluminum profile dust-free chamber | |
| CN201179447Y (en) | Multi-filtering structure for the exhaust flow outside the three-level biological safety cabinet | |
| CN202417562U (en) | Novel tunnel ventilation device | |
| CN102091486B (en) | Straight inserted bag-type air dust removal apparatus | |
| CN104524874A (en) | Edible fungus inoculating room air purification system | |
| CN200972222Y (en) | Combined negative pressure exhaust device | |
| CN204891482U (en) | Dust collector in konjac refined flour workshop | |
| CN211706804U (en) | Novel biological safety cabinet | |
| CN207996530U (en) | A small air filter device for electric cabinet | |
| CN218109352U (en) | High-risk dust removal device | |
| CN213450056U (en) | A pass-through box for toilet | |
| CN207546570U (en) | A kind of ultra-clean workbench with negative pressure | |
| CN111412291A (en) | Single-valve-plate sealing air automatic opening and closing dust remover bypass valve | |
| CN101530816A (en) | Multiple filtration structure for air stream efflux of class III biosafety cabinet | |
| CN214715061U (en) | Gas-driven oil-gas oil-smoke oil mist recovery and purification device | |
| CN202176806U (en) | Inner bypass air sealing valve |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20090916 |