CN101525324A - Method for separating genistein monomer from daidzein monomer - Google Patents
Method for separating genistein monomer from daidzein monomer Download PDFInfo
- Publication number
- CN101525324A CN101525324A CN200910048800A CN200910048800A CN101525324A CN 101525324 A CN101525324 A CN 101525324A CN 200910048800 A CN200910048800 A CN 200910048800A CN 200910048800 A CN200910048800 A CN 200910048800A CN 101525324 A CN101525324 A CN 101525324A
- Authority
- CN
- China
- Prior art keywords
- genistein
- monomer
- solution
- concentration
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for separating a genistein monomer from a daidzein monomer. The method comprises a preparation process of a gold nanotubule membrane, a modification process of the gold nanotubule membrane, a preparation process of an aluminum chelate and a membrane separation process. The method helps realize high-efficiency selective separation of the genistein monomer and the daidzein monomer by utilizing mechanism that the gold nanotubule membrane is modified with p-aminothiophenol so that the membrane is positively charged at a lower pH value, and plays electrostatic repulsion on positively charged genistein and aluminum chelate without repulsion on daidzein which can not form a chelate with the aluminum. The method has the advantages of simple operation, low energy consumption and high efficiency of separation, no pollution, high purity of the monomers separated, industrialized implementation and the like. The method is of great theoretical significance for further research on pharmacological action, mechanism, popularization and application of genistein and daidzein.
Description
Technical field
The present invention relates to the separation method of a kind of genistein and monomer from daidzein monomer, especially relate to the genistein in a kind of soybean isoflavones and the separation method of monomer from daidzein monomer, belong to separation, the purification technique field of natural drug.
Background technology
Studies show that: genistein (another name genistein, English name Genistein) has female hormone and estrogen antagonist character, can suppress colorectal carcinoma, mammary cancer, skin carcinoma, prostate cancer etc. in vivo, and various tumor cell strains is had restraining effect; Have antioxygenation, can prevent postmenopausal osteoporosis; Having the programmed cell death of bringing out, improve anticancer drug effect, suppress effects such as vasculogenesis, is a kind of very potential cancer chemopreventive agent, and its antitumous effect and mechanism are with a wide range of applications.Daidezin (another name daizeol, English name Daidzein) has estrogen-like effects, tangible oxygen lack resistant function is arranged, and Daidezin all is better than genistein to immune promoter action and anti-hemolysis ability.The relative estrogen effect intensity of genistein and Daidezin is respectively 3.1 and 0.26, and genistein and Daidezin are respectively 0.49% and 0.027% to the bonding strength of uterine estrogen receptor.
Genistein and Daidezin all belong to isoflavone like substance, are the main active ingredients of soybean isoflavones.At present, the main obtain manner of soybean isoflavones is to be raw material with the soybean, pretreatment process by different solvents, in conjunction with microwave extraction technology etc., extract the soybean isoflavones crude product, by ultrafiltration and macroporous adsorptive resins, chromatography column etc. the gained crude product is carried out rectification and purification then, utilize chromatography and/or high performance liquid chromatography to carry out each monomer separation at last.Existing separation method is complicated operation not only, the separation costs height, and key is to obtain the sample that high-purity monomer must just might obtain trace through the separating for several times operation, separation efficiency is low, is difficult to be generalized to industrializing implementation.Therefore, study a kind of simple to operate, separation costs is low, efficient is high and isolating monomer purity height, but the genistein of industrializing implementation and the separation method of monomer from daidzein monomer, to further investigate its pharmacological action, mechanism and apply and have the important theoretical meaning.
Summary of the invention
The purpose of this invention is to provide a kind of simple to operate, separation costs is low, efficient is high and isolating monomer purity height, but the separation method of the genistein of industrializing implementation and monomer from daidzein monomer, to overcome above-mentioned existing in prior technology defective.
For achieving the above object, the technical solution used in the present invention is as follows:
The separation method of genistein of the present invention and monomer from daidzein monomer, be that porous polycarbonate (PC) film with 30nm is a mould material, adopting chemical deposition to prepare diameter is gold nano channel membrane about 10nm, utilize p-Mercaptoaniline (PATP) to self-assemble on the gold nano passage hole wall then by gold-sulphur covalent linkage, form the aminoterminal gold nano channel membrane of fine and close individual layer (PATP-Au-Mem), owing to the PATP-Au-Mem terminal amino group than under the low ph condition easily by the protonated gold nano channel membrane lotus positive electricity that makes, the film thing of crossing to lotus positive electricity has the effect of static charge repulsion, therefore the present invention utilizes aluminium and genistein to form the positive charge inner complex again, make it be difficult for seeing through the PATP-Au-Mem film, and monomer from daidzein monomer can not form inner complex with aluminium and easily see through the PATP-Au-Mem film, thereby reaches the purpose of efficient selective separating genistein and monomer from daidzein monomer.
The concrete operations of described separation method are as follows:
A) preparation gold nano channel membrane
Porous polycarbonate (PC) film of 30nm is immersed in the anhydrous methanol, and ultrasonic concussion activates with the impurity that adsorbs on the flush away basement membrane and with film; With the PC film after cleaning is the SnCl of 20～30mM successively in concentration
2Soaking 0.5～1 hour → taking-up water cleaning back in the solution is the Ag (NH of 25～35mM in concentration
3)
2 +Soaking in the solution and soaking 5～10 minutes → taking-up after 10～20 minutes → taking-up water cleans in methyl alcohol is in the gold sodium sulfide deposit solution of 5～10mM, pH=10 in concentration, is 25% HNO again with concentration after the cleaning of 5～6 hours → taking-up of 1～4 ℃ of following chemical gilding water
3The aqueous solution embathed 10～12 hours, and promptly obtaining diameter is the gold nano channel membrane of 8～12nm;
B) modify the gold nano channel membrane
At first with prepared gold nano channel membrane at piranha solution [30%H
2O
2: 98%H
2SO
4=3: 7 (v/v)] in soaked 5～10 minutes, take out water then and clean, immerse concentration and be in p-Mercaptoaniline (PATP) ethanolic soln of 10～30mM and soaked 10～12 hours, film is taken out to dry up at last and gets final product with washed with methanol, nitrogen;
C) inner complex of preparation aluminium
(v/v=1: 1), the concentration that is mixed with genistein is the solution to be separated of 0.5～1mM at first the mixture of genistein and Daidezin to be dissolved in methyl alcohol-phosphate buffer solution; Under the effect of NaCl regulator solution ionic strength, adding concentration in solution to be separated is the AlCl of 0.025～0.1M then
3The aqueous solution at room temperature stirs and got final product in 5～10 minutes, wherein AlCl
3With the mol ratio of genistein be 50: 1～100: 1;
D) separate
Adopt disclosed tripping device among the Chinese patent literature CN1799683: the gold nano channel membrane after will modifying places the centre of U-shaped flow-through cell, and sealed with " zero " type circle, the U-shaped flow-through cell is divided into sample inlet pool and diffusion cell, and Liang Chizhong is equipped with magnetic stirring apparatus and stirs with identical speed; Solution to be separated is placed sample inlet pool, put into the blank solvent that equates with liquor capacity to be separated in the diffusion cell, to guarantee two pond liquid level unanimities; From sample inlet pool and diffusion cell, take a sample, measure the fluorescence intensity of two liquid samples, separate whether fully with monitoring.
Compared with prior art, the present invention has following beneficial effect:
The separation method of genistein of the present invention and monomer from daidzein monomer, modify the gold nano channel membrane by utilizing p-Mercaptoaniline, make it than lotus positive electricity under the low ph condition, thereby the genistein of lotus positive electricity and the inner complex of aluminium had the electrostatic repulsion effect, and to not forming the mechanism of the Daidezin of inner complex without any repulsion with aluminium, realized the efficient selective of genistein and monomer from daidzein monomer is separated, and have simple to operate, isolating energy consumption is low, the efficient height, pollution-free and isolating monomer purity height, but advantages such as industrializing implementation are to the pharmacological action of further investigation genistein and monomer from daidzein monomer, mechanism and apply and have the important theoretical meaning.
Description of drawings
Fig. 1 is scanning electron microscope (SEM) figure on prepared gold nano channel membrane surface.
Fig. 2 is the structural representation of the gold nano channel membrane after modifying, among the figure: a, layer of polycarbonate; B, gold layer.
Fig. 3 is genistein and the aluminium uv-spectrophotometric figure of (b) behind (a) and the chelating before chelating.
Fig. 4 is the structural representation of disclosed tripping device among the Chinese patent literature CN1799683, among the figure: 1, sample inlet pool; 2, the gold nano channel membrane after the modification; 3, diffusion cell; 4, stirrer; 5, agitator.
Fig. 5 is the inner complex (Ge-Al) of genistein and aluminium and the comparison diagram of Daidezin (Da) monomer travelling speed in by the gold nano channel membrane (PATP-Au-Mem) after the p-Mercaptoaniline modification, among the figure: a is the migration of Daidezin (Da) monomer in the PATP-Au-Mem film, and b is the migration of inner complex (Ge-Al) in the PATP-Au-Mem film of genistein and aluminium.
Fig. 6 is the inner complex (Ge-Al) of genistein and aluminium and the comparison diagram of the migration of Daidezin (Da) monomer in the PATP-Au-Mem film and the travelling speed in the gold nano channel membrane (Au-Mem) of unmodified, and among the figure: a is the migration of Daidezin (Da) monomer in the PATP-Au-Mem film, b is the migration of inner complex (Ge-Al) in the PATP-Au-Mem film of genistein and aluminium, c is the migration of inner complex (Ge-Al) in the gold nano channel membrane (Au-Mem) of unmodified of genistein and aluminium, d is the migration of Daidezin (Da) monomer in the gold nano channel membrane (Au-Mem) of unmodified.
Embodiment
The invention will be further described below by embodiment, and its purpose only is better to understand content of the present invention and unrestricted protection scope of the present invention:
Embodiment
A) preparation gold nano channel membrane
Porous polycarbonate (PC) film of 30nm is immersed in the anhydrous methanol, and ultrasonic concussion 5 minutes activates with the impurity that adsorbs on the flush away basement membrane and with film; With the PC film after cleaning is the SnCl of 25mM successively in concentration
2Soak in the solution 45 minutes → be the Ag (NH of 29mM in concentration after taking out water and cleaning 2 times
3)
2 +Soak in the solution 15 minutes → in methyl alcohol, soak after taking out water and cleaning 4 times 5 minutes → to take out in concentration be in the gold sodium sulfide deposit solution (pH=10) of 7.9mM, cleaning after 4 times at 1 ℃ of following chemical gilding 6 hours → take out water is 25% HNO again with concentration
3The aqueous solution embathed 12 hours, and promptly obtaining diameter is the gold nano channel membrane of 10nm; Fig. 1 is scanning electron microscope (SEM) figure on prepared gold nano channel membrane surface, and the aperture R of prepared as seen from the figure gold nano channel membrane is about 10nm.
B) modify the gold nano channel membrane
At first with prepared gold nano channel membrane at piranha solution [30%H
2O
2: 98%H
2SO
4=3: 7 (v/v)] in soaked 5 minutes, take out water then and clean 3 times, immerse concentration and be in p-Mercaptoaniline (PATP) ethanolic soln of 20mM and soaked 12 hours, at last film is taken out and dries up and get final product with washed with methanol 3 times, nitrogen; Fig. 2 is the structural representation of the gold nano channel membrane after modifying, and among the figure: a is a layer of polycarbonate, and b is the gold layer.
C) inner complex of preparation aluminium
Take by weighing the mixture of being made up of 0.027g genistein and 0.025g Daidezin, (v/v=1: 1), the concentration that is mixed with genistein is the solution to be separated of 1mM to be dissolved in methyl alcohol-phosphate buffer solution of 100ml; Under the effect of NaCl regulator solution ionic strength, adding 100ul concentration in solution to be separated is the AlCl of 0.1M
3The aqueous solution at room temperature stirs and got final product in 5 minutes; Fig. 3 is genistein and the aluminium uv-spectrophotometric figure of (b) behind (a) and the chelating before chelating, and as seen from the figure: red shift can take place for genistein and aluminium chelating post-absorption wavelength.
D) separate
Adopt disclosed tripping device (as shown in Figure 4) among the Chinese patent literature CN1799683: the gold nano channel membrane 2 after will modifying places the centre of U-shaped flow-through cell, and sealed with " zero " type circle, the U-shaped flow-through cell is divided into sample inlet pool 1 and diffusion cell 3, be equipped with magnetic stir bar 4 in two ponds, on same magnetic stirring apparatus 5, stir with identical speed; Solution to be separated is placed sample inlet pool, put into the blank solvent (methyl alcohol-phosphate buffer solution) that equates with liquor capacity to be separated in the diffusion cell, to guarantee two pond liquid level unanimities; From sample inlet pool and diffusion cell, take a sample, measure the fluorescence intensity of two liquid samples, separate whether fully with monitoring.
Fig. 5 is the inner complex (Ge-Al) of genistein and aluminium and the comparison diagram of Daidezin (Da) monomer travelling speed in by the gold nano channel membrane (PATP-Au-Mem) after the p-Mercaptoaniline modification, among the figure: a is the migration of Daidezin (Da) monomer in the PATP-Au-Mem film, and b is the migration of inner complex (Ge-Al) in the PATP-Au-Mem film of genistein and aluminium.As seen from the figure: the travelling speed of Daidezin (Da) monomer in PATP-Au-Mem is far longer than the inner complex of genistein and aluminium, and separating genistein and monomer from daidzein monomer that separation method of the present invention can efficient selective are described.
Fig. 6 is the inner complex (Ge-Al) of genistein and aluminium and the comparison diagram of the migration of Daidezin (Da) monomer in the PATP-Au-Mem film and the travelling speed in the gold nano channel membrane (Au-Mem) of unmodified, and among the figure: a is the migration of Daidezin (Da) monomer in the PATP-Au-Mem film, b is the migration of inner complex (Ge-Al) in the PATP-Au-Mem film of genistein and aluminium, c is the migration of inner complex (Ge-Al) in the gold nano channel membrane (Au-Mem) of unmodified of genistein and aluminium, d is the migration of Daidezin (Da) monomer in the gold nano channel membrane (Au-Mem) of unmodified.As seen from the figure: the gold nano channel membrane (Au-Mem) of unmodified does not all have tangible perviousness to the inner complex of Daidezin (Da) monomer and genistein and aluminium, can not realize selective separation between the two, and the present invention is by modifying gold nano channel membrane (Au-Mem) through p-Mercaptoaniline (PATP), make it than lotus positive electricity under the low ph condition, thereby the genistein of lotus positive electricity and the inner complex of aluminium had the electrostatic repulsion effect, and to the Daidezin that can not form inner complex with aluminium without any repulsion, thereby realized the efficient selective of genistein and monomer from daidzein monomer is separated.
Claims (1)
1. the separation method of genistein and monomer from daidzein monomer is characterized in that the concrete operations of described separation method are as follows:
A) preparation gold nano channel membrane
Porous polycarbonate (PC) film of 30nm is immersed in the anhydrous methanol, and ultrasonic concussion activates with the impurity that adsorbs on the flush away basement membrane and with film; With the PC film after cleaning is the SnCl of 20～30mM successively in concentration
2Soaking 0.5～1 hour → taking-up water cleaning back in the solution is the Ag (NH of 25～35mM in concentration
3)
2 +Soaking in the solution and soaking 5～10 minutes → taking-up after 10～20 minutes → taking-up water cleans in methyl alcohol is in the gold sodium sulfide deposit solution of 5～10mM, pH=10 in concentration, is 25% HNO again with concentration after the cleaning of 5～6 hours → taking-up of 1～4 ℃ of following chemical gilding water
3The aqueous solution embathed 10～12 hours, and promptly obtaining diameter is the gold nano channel membrane of 8～12nm;
B) modify the gold nano channel membrane
At first with prepared gold nano channel membrane at piranha solution, i.e. 30%H
2O
2With 98%H
2SO
4By soaking 5～10 minutes in the mixed solution of 3: 7 volume ratio, take out water then and clean, immerse concentration and be in p-Mercaptoaniline (PATP) ethanolic soln of 10～30mM and soaked 10～12 hours, film is taken out to dry up at last and get final product with washed with methanol, nitrogen;
C) inner complex of preparation aluminium
At first the mixture of genistein and Daidezin is dissolved in methyl alcohol and phosphoric acid by in the mixed buffered soln of 1: 1 volume ratio, the concentration that is mixed with genistein is the solution to be separated of 0.5～1mM; Under the effect of NaCl regulator solution ionic strength, adding concentration in solution to be separated is the AlCl of 0.025～0.1M then
3The aqueous solution at room temperature stirs and got final product in 5～10 minutes, wherein AlCl
3With the mol ratio of genistein be 50: 1～100: 1;
D) separate
Adopt disclosed tripping device among the Chinese patent literature CN1799683: the gold nano channel membrane after will modifying places the centre of U-shaped flow-through cell, and sealed with " zero " type circle, the U-shaped flow-through cell is divided into sample inlet pool and diffusion cell, and Liang Chizhong is equipped with magnetic stirring apparatus and stirs with identical speed; Solution to be separated is placed sample inlet pool, put into the blank solvent that equates with liquor capacity to be separated in the diffusion cell, to guarantee two pond liquid level unanimities; From sample inlet pool and diffusion cell, take a sample, measure the fluorescence intensity of two liquid samples, separate whether fully with monitoring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910048800XA CN101525324B (en) | 2009-04-03 | 2009-04-03 | Method for separating genistein monomer from daidzein monomer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910048800XA CN101525324B (en) | 2009-04-03 | 2009-04-03 | Method for separating genistein monomer from daidzein monomer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101525324A true CN101525324A (en) | 2009-09-09 |
| CN101525324B CN101525324B (en) | 2011-09-14 |
Family
ID=41093419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910048800XA Expired - Fee Related CN101525324B (en) | 2009-04-03 | 2009-04-03 | Method for separating genistein monomer from daidzein monomer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101525324B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101718742B (en) * | 2009-11-20 | 2013-04-17 | 上海师范大学 | Method for detecting atrazine |
| CN103113286A (en) * | 2013-02-01 | 2013-05-22 | 上海师范大学 | Method for separating amino acid enantiomer on basis of silicon dioxide nanochannels |
| CN103880721A (en) * | 2014-03-05 | 2014-06-25 | 上海师范大学 | Method of separating chiral drug penicillamine enantiomer based on functional gold nanochannel |
| CN106964324A (en) * | 2017-04-05 | 2017-07-21 | 牡丹江医学院 | A kind of isoflavones isolates and purifies the preparation method with macroporous absorbent resin |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799683A (en) * | 2005-10-20 | 2006-07-12 | 国家海洋局第一海洋研究所 | Separation and selection tool based on Au nano channel array and preparation method thereof |
-
2009
- 2009-04-03 CN CN200910048800XA patent/CN101525324B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101718742B (en) * | 2009-11-20 | 2013-04-17 | 上海师范大学 | Method for detecting atrazine |
| CN103113286A (en) * | 2013-02-01 | 2013-05-22 | 上海师范大学 | Method for separating amino acid enantiomer on basis of silicon dioxide nanochannels |
| CN103113286B (en) * | 2013-02-01 | 2015-04-29 | 上海师范大学 | Method for separating amino acid enantiomer on basis of silicon dioxide nanochannels |
| CN103880721A (en) * | 2014-03-05 | 2014-06-25 | 上海师范大学 | Method of separating chiral drug penicillamine enantiomer based on functional gold nanochannel |
| CN103880721B (en) * | 2014-03-05 | 2016-01-20 | 上海师范大学 | A kind of method of the Jin Ping Mei separating chiral medicine Trolovol enantiomorph based on functionalization |
| CN106964324A (en) * | 2017-04-05 | 2017-07-21 | 牡丹江医学院 | A kind of isoflavones isolates and purifies the preparation method with macroporous absorbent resin |
| CN106964324B (en) * | 2017-04-05 | 2019-10-15 | 牡丹江医学院 | A kind of isoflavones isolates and purifies the preparation method with macroporous absorbent resin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101525324B (en) | 2011-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101525324B (en) | Method for separating genistein monomer from daidzein monomer | |
| CN102218544B (en) | Preparation method and application of metal nanoparticles | |
| Li et al. | Bamboo-inspired design of a stable and high-efficiency catalytic capillary microreactor for nitroaromatics reduction | |
| CN104773796B (en) | Seawater desalination system and method for desalting seawater | |
| CN106006929B (en) | A kind of method of the round-the-clock processing sewage of photoelectrocatalysis film coupling microbiological fuel cell | |
| CN106975373A (en) | Metal organic framework thin film of DNA modification and its preparation method and application | |
| CN105854647A (en) | Sodium polystyrene sulfonate modified metal organic framework film and application thereof | |
| CN102747381A (en) | Method for preparing humic acid through lignite electrochemical oxidation | |
| CN107271529A (en) | A kind of micro-total analysis system chip for the integrated detection of heavy metal ion | |
| CN102163471A (en) | Compound electrode paste for solar battery and preparation method of compound electrode paste | |
| CN208641843U (en) | A kind of mixture extraction separation device | |
| US20180200801A1 (en) | Preparation Method for the Nano-silver Particles | |
| CN102643917B (en) | Lead ion detection test paper and preparation method thereof | |
| CN106517299B (en) | A kind of sheet self assembly basic copper carbonate bouquet and its simple method for preparing | |
| CN101590531A (en) | A kind of method for preparing metal/organic small molecular core/shell type one-dimensional nano-composite material | |
| CN108579468A (en) | A kind of MOP/ hydroxide ceramics composite membrane, preparation method and application | |
| CN207163968U (en) | A kind of micro-total analysis system chip for the integrated detection of heavy metal ion | |
| CN104668577B (en) | A kind of nickel micro-and nano-particles and preparation method thereof | |
| CN101892222B (en) | Reusable plasmid extraction kit | |
| CN106984288B (en) | A kind of preparation method of palm bark surface aurantiin molecular engram adsorbent material | |
| CN102430268B (en) | Filling device of suction nanometer fiber solid phase extraction column and application thereof | |
| CN106380380A (en) | Fullerene derivative and application of fullerene derivative in perovskite solar cells | |
| CN101747275B (en) | Method for separating Huperzine A from Huperziaserrata by foamet | |
| CN103071530A (en) | Application of metal nanoparticles | |
| CN110960577A (en) | Preparation method of tertiary amine alkali component in rhizoma corydalis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110914 Termination date: 20140403 |