CN101524672B - Cyclone separator and spore separation device therewith - Google Patents
Cyclone separator and spore separation device therewith Download PDFInfo
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- CN101524672B CN101524672B CN 200910097391 CN200910097391A CN101524672B CN 101524672 B CN101524672 B CN 101524672B CN 200910097391 CN200910097391 CN 200910097391 CN 200910097391 A CN200910097391 A CN 200910097391A CN 101524672 B CN101524672 B CN 101524672B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The invention discloses a cyclone separator, comprising a primary separator and a secondary separator (8) which are communicated by a channel (9), the bottom of the primary separator is connected with a primary collection tube (6), the bottom of the secondary separator is connected with a secondary collection tube (7); the top surface of the primary separator is provided with an air outlet channel (2), the side wall of the primary separator is tangentially provided with an air inlet channel (1), the air outlet channel (2) and the air inlet channel (1) are both communicated with the inner cavity of the primary separator; an air volume regulator (10) is arranged inside the air inlet channel (1), and the channel (9) is positioned below the air inlet channel (1). The invention also discloses a spore separation device with the cyclone separator, wherein a blower component (300) is connected with the air outlet channel (2) in a sealing way, and a swirling flow feeder component (200) is connected with the air inlet channel (1) in a sealing way. The spore separation device of the invention can efficiently separate solid fermentation fungal spore.
Description
Technical field
The present invention relates to a kind of cyclonic separator and the spore separation device that has this cyclonic separator.
Background technology
Fungus insecticide (Mycoinsecticides) be develop in the world in recent years very rapid, effectively infect the quasi-microorganism sterilant of body (being generally gas living spore estranged) with biocontrol fungi (Fungalbiocontrol agents) as active ingredient; Wherein the purity of conidial powder and quality are directly connected to quality, shelf-life and the field effect (whether blocking sprayer and good disinsection effect) of spore preparation (as finish, suspended emulsion agent etc.); In addition because the impure quality problems such as too much of spore powder cause China's fungus insecticide Industrialization Progress slow; Therefore need separating high-purity and high-quality spore powder efficiently, just can push forward the fast development of fungus insecticide industry comprehensively.
The method of existing separate solid particulate material is usually based on cyclonic separation.Because fungal spore particle diameter small (diameter is often less than 10 microns) easily is suspended in the air at sepn process miospore powder, is difficult to sedimentation and collection, therefore need utilize the centrifugal force of air-flow to reach isolating effect.Shortcomings such as labour intensity was big when the screen cloth separation method had screening, the separation rear impurity is many.Centrifugal separation method is owing to spore and the disengaging fully of solid fermentation substratum particle, so can make separation efficient low.And filter separation method to the performance requriements of filtrate all than higher, be easy to cause the excessive separating effect that influences of filtration resistance in the sepn process.
The gas powder of fungal spore separates employing filtrate method in the tripping device of the patent No. 87107268, but the logical resistance of its Dual-Phrase Distribution of Gas olid is big, and energy consumption is also bigger.The fungal spore separator of the patent No. 01275732.2, earlier the solid medium particle is pulverized in the separate chamber, to be suspended in the spore powder importing cyclonic separator of top, separate chamber with circulating current then, and utilize the rotating centrifugal separation to carry out Dual-Phrase Distribution of Gas olid again and separate with the technology that sack cleaner combines.The advantage of this equipment is simple in structure, and is better to the separation performance of fine dust, but the dead angle that also occurs easily stirring, stirs energy consumption height and the big problem of cloth bag resistance when feeding quantity is big.The fungal spore separator of publication number 200510047737.x circulates solid particle material early stage in separation earlier, and fungal spore is fully separated with substratum, utilizes contain spore air-flow the separated and collected spore of cyclonic separator from introducing again.It is simple in structure, and velocity of separation is fast, and is also more thorough to the separation of fine dust.Abroad other cyclone separator general using fluidized-bed is with the solid fermentation substratum particle spore separation surperficial with it; spore is imported in the cyclonic separator of a plurality of parallel connections with air-flow then; to increase work efficiency; but also be owing to can not solve the escape problem of spore usually, still need set up protector.The employed tornado dust collector of above-mentioned these patents are applicable to that generally purification is greater than the non-sticky of 5-10 micron, the dried dust of non-fiber; But separate solid fermented product fragmentation spore later is during with mycelia, always has in the whirlwind process that both are mixed in together to be difficult to isolating problem, is being mingled with the mycelia fragment in the final feasible spore powder of collecting, and has a strong impact on the purity of spore powder.
Summary of the invention
The technical problem to be solved in the present invention provides the cyclonic separator of a kind of separation efficiency height, safe and low consumption and has the spore separation device of this cyclonic separator.
In order to solve the problems of the technologies described above, the invention provides a kind of cyclonic separator, comprise the main separator and the secondary separator that rely on passage to be connected, the bottom of main separator connects main surge drum, the bottom auxiliary connection surge drum of secondary separator; End face at main separator is provided with the air-out passage, and air intake passage tangentially is set on the sidewall of main separator, air-out passage and air intake passage all with the intracavity inter-connection of main separator; Be provided with blast regulator in the air intake passage, passage is positioned under the air intake passage.
Improvement as cyclonic separator of the present invention: main separator comprises cylindrical body and the cone that is connected successively from top to bottom, end face in cylindrical body is provided with the air-out passage, air intake passage tangentially is set on the sidewall of cylindrical body, air-out passage and air intake passage all with the intracavity inter-connection of cylindrical body; Cylindrical body relies on passage to be connected with secondary separator, in the junction of cylindrical body and cone the ring wing is set, and passage is positioned on the ring wing.
Further improvement as cyclonic separator of the present invention: air intake passage is beloid passage, and the narrow end of air intake passage tangentially links to each other with the sidewall of cylindrical body.
Further improvement as cyclonic separator of the present invention: cone is up big and down small inversion shape cone, and the upper end of cone links to each other with cylindrical body.
Further improvement as cyclonic separator of the present invention: the air-out passage is L-shaped, and the lower end of air-out passage is arranged in the inner chamber of cylindrical body, and the lower end of this air-out passage is positioned under the air intake passage and is positioned on the passage.
The present invention also provides the spore separation device that has above-mentioned cyclonic separator simultaneously, comprises fan assembly and eddy flow feeder assembly; Fan assembly links to each other with the air-out channel seal, and eddy flow feeder assembly links to each other with the air intake passage sealing.
Improvement as spore separation device of the present invention: eddy flow feeder assembly comprises the eddy flow feeder of hollow, at the end face of eddy flow feeder discharge port is set, and opening for feed tangentially is set on the sidewall of eddy flow feeder; Discharge port links to each other with the air intake passage sealing.
Further improvement as spore separation device of the present invention: be provided with screen cloth in the eddy flow feeder, screen cloth is between discharge port and opening for feed.
Further improvement as spore separation device of the present invention: fan assembly comprises fan box, is provided with air inlet and exhaust outlet on fan box, is provided with blower fan in fan box; Air inlet links to each other with the air-out channel seal.
Further improvement as spore separation device of the present invention: be provided with strainer in the fan box, filter bits is between air inlet and blower fan.
Adopt spore separation device of the present invention to carry out the solid fermentation fungal spore separation method, its operation logic is: contain spore solid medium particle in the eddy flow feeder along barrel high speed rotating and mutual frictional impact, because action of gravity, particle can not enter in the cyclonic separator by discharge port; And after spore powder that particle surface adheres to and mycelia fully break away from particle, can tangentially enter in the main separator (being cyclonic separator) by discharge port and air intake passage successively with air-flow.Owing to selected the cyclonic separator that improves design according to vortex pair air-flow principle for use, the air-flow that therefore contains spore powder and mycelia is when acquisition rotatablely moves, and air-flow separately forms the vortex pair motion up and down, forms two air rings up and down.Produce the intensive centrifugation at the vortex pair boundary, thicker mycelia particle enters secondary separator by passage with descending vortical flow to be separated to the inwall (being the inwall of cylindrical body) of main separator behind the barrier effect of the ring wing, enter secondary surge drum at last.Spore powder is brought main surge drum into by downward air-flow.
In the present invention, utilize blast regulator to adjust air velocity, thereby regulate air quantity, can make more thorough that spore powder that particle surface adheres to and mycelia break away from.
The present invention can be used for separating the fungal spore that fermentation backs such as the green green muscardine fungus of chafer, viride, beauveria bassiana produce.
Spore separation device of the present invention has following beneficial effect: (1) safe and low consumption: total system is airtight, separates thoroughly, and purification filtering is complete, operation environment safety, energy-conservation (total power is 1.4kw); (2) simple in structure: major parts is eddy flow feeder assembly, cyclonic separator and fan assembly; (3) apparatus structure compactness, area occupied is little, and for example floor space only is 1000 * 500 * 1200mm.Adopting spore separation device of the present invention to carry out solid fermentation fungal spore separates, have the following advantages: high efficiency separation, velocity of separation is fast: by the master, the setting of secondary separator, can be with the separating substances and the collection of Different Weight, for example mycelia can be separated respectively with spore and collect, improved the collection purity of spore, make the separation purity height.
Description of drawings
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in further detail.
Fig. 1 is the synoptic diagram after the cyclonic separator biopsy cavity marker devices of the present invention;
Fig. 2 is the partial schematic diagram of overlooking of Fig. 1;
Fig. 3 is the structural representation of spore separation device of the present invention;
Fig. 4 is the rotation synoptic diagram that A-A cuts open among Fig. 3.
Embodiment
End face in cylindrical body 3 is provided with the air-out passage 2 that stainless steel is made, and this air-out passage 2 is L-shaped, and air-out passage 2 is fixedly linked hermetically with the end face of cylindrical body 3, and the lower end 11 of air-out passage 2 is arranged in the inner chamber of cylindrical body 3.Be provided with otch on the sidewall of cylindrical body 3, this otch parallels with the tangent line of cylindrical body 3 sidewalls, and this otch is near the end face of cylindrical body 3; The air intake passage of being made by stainless steel 1 is a beloid passage, and the cut sealing ground on the narrow end of this air intake passage 1 and cylindrical body 3 sidewalls is connected; This air intake passage 1 is perpendicular to the sidewall of cylindrical body 3, thus tangential the linking to each other and the intracavity inter-connection of air intake passage 1 and cylindrical body 3 of having realized air intake passage 1 and cylindrical body 3 sidewalls.Be provided with blast regulator 10 in the air intake passage 1.The lower end 11 of air-out passage 2 is positioned at the lower end of air intake passage 1, and promptly the lower end 11 of air-out passage 2 is lower than the otch on cylindrical body 3 sidewalls.In the junction of cylindrical body 3 and cone 5 the ringwise ring wing 4 is set.The bottom of cone 5 links to each other with main surge drum 6.
Eddy flow feeder assembly 200 comprises the eddy flow feeder 22 that being of hollow is cylinder-shaped, at the end face of eddy flow feeder 22 discharge port 20 is set; Be provided with otch on the sidewall of eddy flow feeder 22, this otch parallels with the tangent line of eddy flow feeder 22 sidewalls, and this otch is near the bottom surface of eddy flow feeder 22; Cut sealing ground on opening for feed 23 and eddy flow feeder 22 sidewalls is connected, and this opening for feed 23 is perpendicular to the sidewall of eddy flow feeder 22; Thereby tangential the linking to each other and the intracavity inter-connection of opening for feed 23 and eddy flow feeder 22 of having realized opening for feed 23 and eddy flow feeder 22 sidewalls.Be provided with screen cloth 21 in eddy flow feeder 22, screen cloth 21 is between discharge port 20 and opening for feed 23; Be that screen cloth 21 is positioned on the opening for feed 23, under the discharge port 20.Discharge port 20 links to each other with air intake passage 1 sealing by connecting leg 42.The bottom surface of eddy flow feeder 22 relies on screw thread to link to each other with the sidewall of eddy flow feeder 22.
Utilize spore separation device of the present invention to carry out solid fermentation fungal spore and separate, its working process is specific as follows:
1, will contain spore solid medium oven dry and its rolling loose into particle; Granular size generally is controlled in the 10mm.And the above-mentioned container that contains spore solid medium particle (hereinafter to be referred as particle) will be housed link to each other with opening for feed 23.
Start blower fan 32 by control components and parts 34, the suction that utilizes blower fan 32 to produce, above-mentioned particle enters in the eddy flow feeder 22 with tangential direction through opening for feed 23 with air-flow.Can pass through blast regulator 10, thereby change air velocity, air velocity generally is controlled at 8.5～44.5 meter per seconds.
2, particle in eddy flow feeder 22 along the barrel high speed rotating of eddy flow feeder 22 and frictional impact mutually, spore powder and mycelia enter in the cylindrical body 3 with tangential direction through screen cloth 21, discharge port 20, connecting leg 42 and air intake passage 1 after breaking away from from particle successively.
Particle can't rise to discharge port 20 under the effect of gravity, and screen cloth 21 also plays the effect that stops that particle rises.
3, spore powder and mycelia enter in the cylindrical body 3 with tangential direction and further separate, and be specific as follows:
The air-flow that contains spore powder and mycelia is when acquisition rotatablely moves, and air-flow separately forms the vortex pair motion up and down, forms two air rings up and down.Produce the intensive centrifugation at the vortex pair boundary, thicker mycelia particle enters secondary separator 8 by passage 9 with descending vortical flow to be separated to the inwall of cylindrical body 3 behind the barrier effect of the ring wing 4, enter secondary surge drum 7 at last.Most spore powder are brought main surge drum 6 into by downward air-flow.
The spore powder of few part along with air-flow successively through air-out passage 2, connecting leg 41, air inlet 30, and then discharged through exhaust outlet by blower fan 32 through after the filtration of filter 31.The effect of strainer 31 is to avoid entering in the extraneous air-flow being mingled with spore powder.
4, when no longer including spore powder and mycelia in the eddy flow feeder 22 and kick up, can close blower fan 32, thereby finish sepn process because of whirlwind.Open the spiral cover of eddy flow feeder 22 bottoms, outwell remaining particle.
Test one, from the barley solid medium, separate Metarhizium anisopliae:
Claim 324.4 grams dried Metarhizium anisopliae ARSEF 576 barley solid fermentation substratum (water content 8% ± 1%), rolling is loose into particle and is placed in the clean container; Adopt above-mentioned separation method, utilize blast regulator 10 to adjust air velocity to 20.9 meter per second earlier.
Separate carry out 30 seconds after, adjust air velocity to 40 meter per second, purpose be make spore powder and mycelia from particle break away from more thorough.The air velocity that keeps 40 meter per seconds separated 1 minute again; Close blower fan 32, finish whole mask work.
(spore content is: 0.61 ± 0.15 * 10 to obtain 12.7 gram muscardine spore powder at main surge drum 6
11/ gram powder), adopt the back that suspends to detect with the blood counting chamber method.In secondary surge drum 7, obtain 2.6 gram green muscardine fungus mycelia.
Experiment two: from the rice solid medium, separate viride:
Claim 832 grams dried viride CICC 40495 rice solid fermentation substratum (water content 7.5% ± 1%), rolling is loose into particle and is placed in the clean container, adopts above-mentioned separation method, adjusts air velocity to 20 meter per second.After separation is carried out 1 minute, close blower fan 32, finish whole mask work.
(spore content is: 0.63 ± 0.01 * 10 to obtain 15 gram viride spore powder in main surge drum 6
11/ gram powder).In secondary surge drum 7, obtain 12 gram viride mycelia.
Experiment three: from the rice solid medium, separate beauveria bassiana
Claim 354.2 grams dried beauveria bassiana ARSEF 734 rice solid fermentation substratum (water content 7.4% ± 1%), rolling is loose into particle and is placed in the clean container, adopts above-mentioned separation method, adjusts air velocity to 12.9 meter per second earlier.After separation is carried out 1 minute; Close blower fan 32, finish whole mask work.
(spore content is: 1.73 ± 0.05 * 10 to obtain 5.4 gram beauveria bassiana spore powder in main surge drum 6
11/ gram powder).In secondary surge drum 7, obtain 0.4 gram beauveria bassiana mycelia.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (9)
1. cyclonic separator is characterized in that: comprise the main separator and the secondary separator (8) that rely on first channel (9) to be connected, the bottom of main separator connects main surge drum (6), the bottom auxiliary connection surge drum (7) of secondary separator (8); Described main separator comprises cylindrical body (3) and the cone (5) that is connected successively from top to bottom, end face in cylindrical body (3) is provided with air-out passage (2), air intake passage (1) tangentially is set on the sidewall of cylindrical body (3), air-out passage (2) and air intake passage (1) all with the intracavity inter-connection of cylindrical body (3); Cylindrical body (3) relies on first channel (9) to be connected with secondary separator (8), and the ring wing (4) is set in the junction of cylindrical body (3) and cone (5), and first channel (9) is positioned on the ring wing (4), and first channel (9) is positioned under the air intake passage (1); Be provided with blast regulator (10) in the air intake passage (1).
2. cyclonic separator according to claim 1 is characterized in that: described air intake passage (1) is beloid passage, and the narrow end of described air intake passage (1) tangentially links to each other with the sidewall of cylindrical body (3).
3. cyclonic separator according to claim 1 and 2 is characterized in that: described cone (5) is up big and down small inversion shape cone, and the upper end of described cone (5) links to each other with cylindrical body (3).
4. cyclonic separator according to claim 3, it is characterized in that: described air-out passage (2) is L-shaped, the lower end (11) of air-out passage (2) is arranged in the inner chamber of cylindrical body (3), and the lower end (11) of this air-out passage (2) is positioned under the air intake passage (1) and is positioned on the first channel (9).
5. have the spore separation device of any one cyclonic separator in the claim 1～4, it is characterized in that: comprise fan assembly (300) and eddy flow feeder assembly (200); Described fan assembly (300) links to each other with air-out passage (2) sealing, and eddy flow feeder assembly (200) links to each other with air intake passage (1) sealing.
6. spore separation device according to claim 5, it is characterized in that: described eddy flow feeder assembly (200) comprises the eddy flow feeder (22) of hollow, end face at eddy flow feeder (22) is provided with discharge port (20), and opening for feed (23) tangentially is set on the sidewall of eddy flow feeder (22); Described discharge port (20) links to each other with air intake passage (1) sealing.
7. spore separation device according to claim 6 is characterized in that: be provided with screen cloth (21) in the eddy flow feeder (22), described screen cloth (21) is positioned between discharge port (20) and the opening for feed (23).
8. according to claim 6 or 7 described spore separation devices, it is characterized in that: described fan assembly (300) comprises fan box (33), on fan box (33), be provided with air inlet (30) and exhaust outlet, in fan box (33), be provided with blower fan (32); Air inlet (30) links to each other with air-out passage (2) sealing.
9. spore separation device according to claim 8 is characterized in that: be provided with strainer (31) in the described fan box (33), strainer (31) is positioned between air inlet (30) and the blower fan (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910097391 CN101524672B (en) | 2009-04-13 | 2009-04-13 | Cyclone separator and spore separation device therewith |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910097391 CN101524672B (en) | 2009-04-13 | 2009-04-13 | Cyclone separator and spore separation device therewith |
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| CN101524672A CN101524672A (en) | 2009-09-09 |
| CN101524672B true CN101524672B (en) | 2010-09-08 |
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| CN 200910097391 Expired - Fee Related CN101524672B (en) | 2009-04-13 | 2009-04-13 | Cyclone separator and spore separation device therewith |
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Cited By (1)
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| CN103396939A (en) * | 2013-08-06 | 2013-11-20 | 当阳市森林病虫害防治检疫站 | Spore powder collection method, spore powder collection apparatus and brushing machine |
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| CN101878701A (en) * | 2010-08-10 | 2010-11-10 | 无锡五芝源生物农业科技有限公司 | Ganoderma lucidum spore collector |
| CN102225394A (en) * | 2011-04-29 | 2011-10-26 | 杨国旺 | Wind separating screen device and separating method thereof |
| CN102965277A (en) * | 2012-11-27 | 2013-03-13 | 北京市西山试验林场 | Cross-flow type cyclone-cloth bag combined dust collector system for bio-active spore powder |
| CN103962249A (en) * | 2013-01-29 | 2014-08-06 | 昆山尚达智机械有限公司 | Cyclone separator |
| CN104630020B (en) * | 2013-11-07 | 2016-08-24 | 中国科学院天津工业生物技术研究所 | A kind of solid-state biological reaction apparatus and the method preparing filamentous bacteria spore with this device |
| CN104630049A (en) * | 2015-01-23 | 2015-05-20 | 中国科学院合肥物质科学研究院 | Full-automatic separation device and separation method of solid fermentation fungus spores |
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| GB201904083D0 (en) * | 2019-03-25 | 2019-05-08 | Ge Healthcare Bio Sciences Ab | A bioreactor system |
| CN116925897B (en) * | 2023-09-15 | 2023-12-05 | 龙牌食品股份有限公司 | Aeration-cooling device for making yeast by disc |
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2009
- 2009-04-13 CN CN 200910097391 patent/CN101524672B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396939A (en) * | 2013-08-06 | 2013-11-20 | 当阳市森林病虫害防治检疫站 | Spore powder collection method, spore powder collection apparatus and brushing machine |
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