CN101520444A - Method for identifying Lonicera confusa and honeysuckle flower and application thereof - Google Patents
Method for identifying Lonicera confusa and honeysuckle flower and application thereof Download PDFInfo
- Publication number
- CN101520444A CN101520444A CN200910037372A CN200910037372A CN101520444A CN 101520444 A CN101520444 A CN 101520444A CN 200910037372 A CN200910037372 A CN 200910037372A CN 200910037372 A CN200910037372 A CN 200910037372A CN 101520444 A CN101520444 A CN 101520444A
- Authority
- CN
- China
- Prior art keywords
- solution
- lonicera confusa
- honeysuckle
- acid
- reference substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a method for identifying Lonicera confusa and honeysuckle flower, which differentiates the Lonicera confusa and the honeysuckle flower by a thin-layer chromatography. The invention further adopts a high performance liquid chromatography to identify the Lonicera confusa and the honeysuckle flower. The identifying method can be used for evaluating the truth and quality of honeysuckle flower medicament or Lonicera confusa medicament, differentiating the honeysuckle flower medicament and the Lonicera confusa medicament, and evaluating the quality of batch charging medicaments in preparations to ensure stability, evenness and controllability of the medicaments. The method has the advantages of simple and convenient operation, good repeatability, strong intuition and the like.
Description
Technical field
The invention discloses a kind of method and application thereof of differentiating Lonicera confusa and honeysuckle, belong to the Pharmaceutical Analysis field.
Background technology
Lonicera confusa records as a source in the honeysuckle in each edition before the version Pharmacopoeia of the People's Republic of China in 2005,2005 the version Pharmacopoeia of the People's Republic of China (one one) the two is distinguished, but both source section symbolic animal of the birth year are same, kind is similar, nature and flavor, discriminating, function and cure mainly, usage is identical with consumption, the assay item is the high effective liquid chromatography for measuring chlorogenic acid content, method and limit are identical, difference is that honeysuckle has increased galuteolin assay item, but the bibliographical information Lonicera confusa also contains galuteolin.According to bibliographical information, the main cause of standard apportion is " the multi-source kind that Chinese crude drug component content difference is bigger is by the principle of one of thing apportion progressively ", represents its chemical constitution or content of effective difference.
The medicinal material that feeds intake in the compound Chinese medicinal preparation is Lonicera confusa or honeysuckle, can't distinguish always.State Food and Drug Administration stipulates to feed intake fixedly kind of medicinal material, thereby guarantees stable, the homogeneous of the quality of the pharmaceutical preparations, and therefore distinguishing Lonicera confusa and honeysuckle is the technical matters of being badly in need of solution.
Summary of the invention
Technical matters to be solved by this invention is the difficulty that overcomes prior art, and purpose is to provide a kind of method of differentiating Lonicera confusa and honeysuckle efficiently, reliably.
Another object of the present invention provides the application of said method.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of method of differentiating Lonicera confusa and honeysuckle is to utilize thin-layered chromatography that Lonicera confusa and honeysuckle are distinguished; The chromatographic condition of described thin-layered chromatography is: the methanol solution that reference substance solution chlorogenic acid and caffeic acid mix, and each concentration of component is 1mg/ml, and need testing solution is the methanol solution of medicinal material; Thin layer plate is for being the silica gel H plate or the G plate of binder with the sodium carboxymethyl cellulose; Point sample amount 0.1～20 μ l; Developping agent is the upper solution or the chloroform-methanol-formic acid (9:1:0.5) of butyl acetate-formic acid-water (7:2.5:1～3); Inspect under the ultraviolet lamp 365nm.
The result who inspects is: in the test sample chromatogram, with the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color.In the Lonicera confusa test sample thin-layer chromatography, can see a series of feature spot, by with chlorogenic acid, the contrast of caffeic acid reference substance, two development system contrasts, can clearly see that 3 feature spots that Lonicera confusa has are different from the blue-fluorescence spot of honeysuckle, so can distinguish honeysuckle and Lonicera confusa.
Further, in conjunction with high performance liquid chromatography Lonicera confusa and honeysuckle are distinguished:
High-efficient liquid phase chromatogram condition is: be filling agent with the octadecylsilane chemically bonded silica; With acetonitrile-phosphoric acid solution (12:88) is moving phase; The detection wavelength is 310～340nm; Theoretical cam curve is calculated by the caffeic acid peak and is not less than 1000.
The preparation of reference substance solution: precision takes by weighing chlorogenic acid and the caffeic acid reference substance is an amount of, puts in the brown bottle, adds 50% methanol solution and makes the solution that every 1ml contains chlorogenic acid 40 μ g, caffeic acid 8 μ g, promptly.
The preparation of need testing solution: get this product powder (crossing sieve No. four) 0.1～10g, the accurate title, decide, and puts in the tool plug conical flask, the accurate 50% methyl alcohol 50ml that adds claims to decide weight, sonicated (power 250W, frequency 35kHz) 30 minutes, put coldly, claim to decide weight again, supply the weight that subtracts mistake with 50% methyl alcohol, shake up, filter, precision is measured subsequent filtrate 5ml, puts in the brown measuring bottle of 25ml, adds 50% methyl alcohol to scale, shake up, promptly.
Determination method: accurate respectively reference substance solution and each 5～10 μ l of need testing solution of drawing, injecting chromatograph is measured, promptly.This product is pressed dry product and is calculated, the chlorogenic acid (C in the 10 batches of Lonicera confusas and the honeysuckle
16H
18O
9) content of 2.4～3.9%, two kinds of medicinal materials of content is suitable; Caffeic acid (the C of Lonicera confusa
9H
8O
4) content range is greatly about 0.1%～0.23%, and caffeic acid content is about 0.02%～0.03% in the honeysuckle, the caffeic acid content of Lonicera confusa is about 10 times of honeysuckle.
The present invention also provides the application of above-mentioned discrimination method, is to be applied to differentiate the pharmaceutical preparation that contains Lonicera confusa or honeysuckle.
Compared with prior art, the present invention has following beneficial effect:
Discrimination method of the present invention can be used for the evaluation of honeysuckle or the Lonicera confusa medicinal material true and false and quality quality, can be used for distinguishing traditional Chinese medicine honeysuckle and Lonicera confusa medicinal material, also can be used for feeding intake in the preparation quality evaluation of medicinal material guarantees stable, the homogeneous of its quality, and is controlled.Advantage such as that method of the present invention has is easy and simple to handle, favorable reproducibility, intuitive are strong.
Description of drawings
Fig. 1 and Fig. 2 are Lonicera confusa of the present invention and the qualitative collection of illustrative plates of honeysuckle thin-layer chromatography, put under the ultraviolet lamp 365nm and inspect, the point sample order: 1～2. honeysuckle need testing solution (is followed successively by control medicinal material 121060-200504, commercially available product), 3. chlorogenic acid reference substance, 4. caffeic acid reference substance, 5～14.10 batches of Lonicera confusa need testing solutions.Fig. 1 developping agent is the upper solution of butyl acetate-formic acid-water (7:2.5:1), and Fig. 2 developping agent is chloroform-methanol-formic acid (9:1:0.5).A, B, C, D are the feature spot among the figure, and wherein A is that chlorogenic acid, B are caffeic acid, and two development system contrasts can clearly see that blue-fluorescence spot B, C, D are for distinguishing 3 feature blue-fluorescence spots of honeysuckle and Lonicera confusa.
Fig. 3, Fig. 4, Fig. 5 are the quantitative collection of illustrative plates of high performance liquid chromatography of the present invention, are respectively chlorogenic acid, caffeic acid mixing reference substance, Lonicera confusa medicinal material and honeysuckle control medicinal material liquid chromatogram; 33 are chlorogenic acid among the figure, and 34 are caffeic acid.
Embodiment
Below further specify technical scheme of the present invention by specific embodiment.
1 instrument and reagent
1.1 instrument: wear peace Dionex company's high performance liquid chromatograph (ASI-100 automatic sampler, ATH-585 column oven, P680 quaternary gradient pump, PDA-100 detecting device);
Chromatographic column: Dikma PLATISIL ODS C
18, 250mm * 4.6mm, 5 μ m;
Prefabricated silica gel H thin plate, G thin plate (the biochemical plastic molding and processing plant of Taizhou city, glass chip 20 * 20cm Zhejiang Province road and bridge tetramethyl);
The automatic point sample instrument of CAMAG AUTOMATIC TLC SAMPLER thin layer, CAMAG TLCREPROSTAR thin layer automated imaging system, CAMAG double flute expansion cylinder (Switzerland CAMAG company);
1.2 reagent: the 10 batches of Lonicera confusa medicinal materials and kouyanqing granules, honeysuckle control medicinal material provide by Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd..Liquid chromatography agents useful for same acetonitrile is chromatographically pure in the experiment, and it is pure that all the other agents useful for same are analysis, and water is ultrapure water.
2 methods and result
2.1 the thin layer of Lonicera confusa is differentiated in the kouyanqing granules: get 10 bags of this product, porphyrize is got about 10g, the accurate title, decide, put in the tool plug conical flask accurate methyl alcohol 50ml, the close plug of adding, claim to decide weight, sonicated (power 360W, frequency 35kHz) 30 minutes, put coldly, claim again to decide weight, supply the weight that subtracts mistake with methyl alcohol, shake up, filter, precision is measured subsequent filtrate 10ml, decompression and solvent recovery is to doing, and residue adds the about 5ml dissolving of water, quantitatively is transferred to solid phase extraction column (filler: C
18, specification: 6ml 500mg), adds water 15ml gradation drip washing, leacheate discards, and uses methyl alcohol 15ml gradation wash-out again, collects eluent, and decompression and solvent recovery is to doing, residue quantitatively adds methyl alcohol 2ml, makes dissolving fully, filters with 0.45 μ m miillpore filter, as the finished product need testing solution.Other gets Lonicera confusa or traditional Chinese medicine honeysuckle powder 0.2g, adds methanol extraction, filters, and filtrate is as the medicinal material need testing solution.Get chlorogenic acid, caffeic acid reference substance again, add methyl alcohol and make the solution that every 1ml contains 1mg, as mixing reference substance solution.Test according to thin-layered chromatography (Pharmacopoeia of the People's Republic of China appendix VI B in 2005), draw each 10 μ l of finished product need testing solution, above-mentioned reference substance solution, medicinal material need testing solution and finished product need testing solution, put respectively in same be on the silica gel H thin layer plate of binder with the sodium carboxymethyl cellulose, upper solution or chloroform-methanol-formic acid (9:1:0.5) with butyl acetate-formic acid-water (7:2.5:1～3) is developping agent respectively, launch about 15 centimetres, take out, dry, put under the ultraviolet lamp (365nm) and inspect.See Fig. 1 with the former gained image of developping agent, see Fig. 2 with developping agent latter gained image.In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color.
2.2 efficient liquid phase chromatographic analysis
The preparation of need testing solution: the material powder of getting it filled (crossing sieve No. four) 0.5g, the accurate title, decide, and puts in the tool plug conical flask, the accurate 50% methyl alcohol 50ml that adds claims to decide weight, sonicated (power 250W, frequency 35kHz) 30 minutes, put coldly, claim to decide weight again, supply the weight that subtracts mistake with 50% methyl alcohol, shake up, filter, precision is measured subsequent filtrate 2ml, puts in the brown measuring bottle of 10ml, adds 50% methyl alcohol to scale, shake up, promptly.
The preparation of reference substance solution: precision takes by weighing chlorogenic acid and the caffeic acid reference substance is an amount of, puts in the brown bottle, adds 50% methanol solution and makes the solution that every 1ml contains chlorogenic acid 40 μ g, caffeic acid 8 μ g, promptly.
Chromatographic condition: chromatographic column is Dikma PLATISIL ODS C18,250mm * 4.6mm, 5 μ m; Moving phase is that acetonitrile-0.4% phosphoric acid solution (pH ≈ 2.50) (12: 88) is moving phase, flow velocity 1.0ml/min, and the detection wavelength is 327nm.
Determination method: accurate reference substance and the need testing solution 10 μ l of drawing, sample introduction is measured chlorogenic acid and caffeinic content in accordance with the law.
Chlorogenic acid and caffeic acid mixing reference substance chromatogram are seen Fig. 3, and Lonicera confusa medicinal material liquid chromatogram is seen Fig. 4, and honeysuckle control medicinal material liquid chromatogram is seen Fig. 5.
Chlorogenic acid all reaches the requirement that pharmacopeia is stipulated in 10 batches of Lonicera confusas〉1.5%; The high-load of caffeic acid is 0.23%, and minimum content is 0.10%.By comparative analysis, the chlorogenic acid content about 2.4～3.9% of Lonicera confusa and honeysuckle, the content of two kinds of medicinal materials is suitable.Caffeic acid (the C of 10 batches of Lonicera confusas
9H
8O
4) content range is greatly about 0.1%～0.23%, and caffeic acid content is about 0.02%～0.03% in the honeysuckle, the caffeic acid content of Lonicera confusa is 5～10 times of honeysuckle.
2.3 accurate caffeic acid, the chlorogenic acid reference substance mixed solution 10 μ l of drawing of precision test, under above chromatographic condition, repeat sample introduction 6 times continuously, measure in accordance with the law, the relative standard deviation of calculating caffeic acid, chlorogenic acid reference substance peak area is respectively 0.51% and 0.88%, and show: the precision of this method is good.
2.4 the replica test precision takes by weighing 6 parts of same lot number medicinal powders, preparation method and chromatographic condition time-and-motion study according to above-mentioned need testing solution, sample introduction 10 μ l, the relative standard deviation of calculating caffeic acid, chlorogenic acid peak area is respectively 1.65% and 2.31%, shows the good reproducibility of this method.
2.5 middle precision test: precision takes by weighing 2 parts of same lot number medicinal powders, respectively under not same date, different instrument variable condition, carry out time-and-motion study by 2.2 efficient liquid phase chromatographic analysis in accordance with the law, calculate caffeic acid and chlorogenic acid contents and relative standard deviation.The result is same date RSD=1.33% not, and different instrument RSD=0.52% show: precision in the middle of this method has preferably.
2.6 average recovery test: the accurate respectively medicinal powder that takes by weighing known caffeic acid, chlorogenic acid content is an amount of, precision adds a certain amount of reference substance respectively again, make caffeic acid in the need testing solution, chlorogenic acid concentration respectively at high, medium and low three concentration ranges of caffeic acid, chlorogenic acid typical curve, 3 parts of each concentration operation repetitives, by the time-and-motion study in accordance with the law of 2.2 efficient liquid phase chromatographic analysis, calculate recovery rate.The average recovery rate of caffeic acid, chlorogenic acid is respectively 99.6% and 104.7% as a result, and RSD is respectively 2.16% and 3.26%.Show: this method recovery is good, i.e. accuracy is good.
Claims (3)
1. a method of differentiating Lonicera confusa and honeysuckle is to utilize thin-layered chromatography that Lonicera confusa and honeysuckle are distinguished;
The chromatographic condition of described thin-layered chromatography is: the methanol solution that reference substance solution chlorogenic acid and caffeic acid mix, and each concentration of component is 1mg/ml, and need testing solution is the methanol solution of medicinal material; Thin layer plate is for being the silica gel H plate or the G plate of binder with the sodium carboxymethyl cellulose; Point sample amount 0.1～20 μ l; Developping agent is the upper solution or the chloroform-methanol-formic acid (9:1:0.5) of butyl acetate-formic acid-water (7:2.5:1～3); Inspect under the ultraviolet lamp 365nm.
2. discrimination method as claimed in claim 1 is characterized in that further adopting high performance liquid chromatography that Lonicera confusa and honeysuckle are distinguished;
High-efficient liquid phase chromatogram condition is: be filling agent with the octadecylsilane chemically bonded silica; With acetonitrile-phosphoric acid solution (12:88) is moving phase; Sample size: 5～10 μ l; The detection wavelength is 310～340nm; Theoretical cam curve is calculated by the caffeic acid peak and is not less than 1000;
Reference substance solution: 50% methanol solution of chlorogenic acid and caffeinic mixing, wherein every ml contain chlorogenic acid 40 μ g, caffeic acid 8 μ g; Need testing solution: the solution of 50% methyl alcohol of medicinal material;
3. the application of claim 1 or 2 described discrimination methods is to be applied to differentiate the pharmaceutical preparation that contains Lonicera confusa or honeysuckle.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910037372A CN101520444A (en) | 2009-02-24 | 2009-02-24 | Method for identifying Lonicera confusa and honeysuckle flower and application thereof |
| CN2009101399094A CN101628023B (en) | 2009-02-24 | 2009-07-10 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910037372A CN101520444A (en) | 2009-02-24 | 2009-02-24 | Method for identifying Lonicera confusa and honeysuckle flower and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101520444A true CN101520444A (en) | 2009-09-02 |
Family
ID=41081147
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910037372A Pending CN101520444A (en) | 2009-02-24 | 2009-02-24 | Method for identifying Lonicera confusa and honeysuckle flower and application thereof |
| CN2009101399094A Active CN101628023B (en) | 2009-02-24 | 2009-07-10 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101399094A Active CN101628023B (en) | 2009-02-24 | 2009-07-10 | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN101520444A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277721A (en) * | 2015-10-14 | 2016-01-27 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing honeysuckle from wild honeysuckle flowers |
| CN106706626A (en) * | 2017-02-17 | 2017-05-24 | 西南民族大学 | Ingredient identification and antibacterial activity evaluation method of cliff honeysuckle |
| CN108445108A (en) * | 2018-04-23 | 2018-08-24 | 山东省食品药品检验研究院 | A kind of discrimination method of honeysuckle and Honeysuckle flower |
| CN114689753A (en) * | 2022-03-31 | 2022-07-01 | 广东一方制药有限公司 | Method for identifying honeysuckle in different Yuanyuan mountain |
| CN114965842A (en) * | 2022-06-01 | 2022-08-30 | 广州市药品检验所 | Method for detecting overall quality of Kouyanqing and application thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539613A (en) * | 2012-01-04 | 2012-07-04 | 北京知蜂堂蜂产品有限公司 | TLC (Thin Layer Chromatography) identification method of true propolis and false propolis |
| CN110646521A (en) * | 2018-06-26 | 2020-01-03 | 北京紫云腾中药饮片有限公司 | Detection method of prepared rehmannia root decoction pieces |
| CN109030666B (en) * | 2018-10-09 | 2022-01-11 | 山东中医药大学 | Method for identifying honeysuckle and lonicera confusa by utilizing high performance liquid chromatography |
-
2009
- 2009-02-24 CN CN200910037372A patent/CN101520444A/en active Pending
- 2009-07-10 CN CN2009101399094A patent/CN101628023B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277721A (en) * | 2015-10-14 | 2016-01-27 | 广州白云山和记黄埔中药有限公司 | Method for distinguishing honeysuckle from wild honeysuckle flowers |
| CN106706626A (en) * | 2017-02-17 | 2017-05-24 | 西南民族大学 | Ingredient identification and antibacterial activity evaluation method of cliff honeysuckle |
| CN108445108A (en) * | 2018-04-23 | 2018-08-24 | 山东省食品药品检验研究院 | A kind of discrimination method of honeysuckle and Honeysuckle flower |
| CN114689753A (en) * | 2022-03-31 | 2022-07-01 | 广东一方制药有限公司 | Method for identifying honeysuckle in different Yuanyuan mountain |
| CN114965842A (en) * | 2022-06-01 | 2022-08-30 | 广州市药品检验所 | Method for detecting overall quality of Kouyanqing and application thereof |
| CN114965842B (en) * | 2022-06-01 | 2023-09-22 | 广州市药品检验所 | Stomatitis clearing integral quality detection method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101628023B (en) | 2012-09-05 |
| CN101628023A (en) | 2010-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101628023B (en) | Method for distinguishing lonicera hypoglauca miq and honeysuckle and application thereof | |
| CN102119961B (en) | Detection method of compound danshen dripping pills | |
| CN101647829B (en) | Quality control method of ginkgolide injection | |
| CN105259295A (en) | Quality detection method for ginseng, cassia twig and poria cocos oral solution | |
| CN103149320A (en) | Quality control method of loins-strengthening and kidney-invigorating medicine | |
| CN113466355A (en) | Construction method of high performance liquid phase characteristic spectrum of caulis sinomenii | |
| CN103969346A (en) | Panax notoginseng saponin component analysis method | |
| CN104034834A (en) | Method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials and application of method | |
| CN106124684B (en) | A kind of qualitative and quantitative detection method of granatum anti-diarrhea medicinal powder | |
| CN104833736A (en) | Fast qualitative and quantitative detection method for 25-ingredient coral pills through quantitative analysis of multi-components by single-marker | |
| CN103316102B (en) | Detection method for external traditional Chinese medicine preparation for treating haemorrhoids | |
| CN106770764B (en) | A kind of content assaying method of Xue bijing intermediate multi-target ingredient | |
| CN104897796A (en) | Method for determining oleanolic acid content and ursolic acid content in sesame oil through high performance liquid chromatography method | |
| CN101979027B (en) | Method for detecting Jinze coronary disease capsules | |
| CN106680414A (en) | Detection method of compound ardisia japonica tablet | |
| CN104345108B (en) | Qualitative quantitative determination method for liver-heat-clearing tablet | |
| CN102552655A (en) | Detection method for Chinese herbal preparation of particles capable of clearing heat and detoxicating | |
| CN103512979B (en) | Detection method of pharmaceutical composition Zuozhudaxi | |
| CN103675192A (en) | Detection method of inflammation-diminishing compound pierasma quassioides benn capsule | |
| CN103454374A (en) | Quality control method of bone rehabilitation medicine | |
| CN102608250A (en) | Rapid detection method for Jinfangganmao granules | |
| CN108037200B (en) | Quality detection method of kidney nourishing and tranquilizing pills | |
| CN102854283B (en) | Detection method of polygala arvensis | |
| CN105974025A (en) | Detection method of traditional Chinese preparation for treating stomach illness | |
| CN105717246B (en) | Method for simultaneously detecting content of five effective ingredients in Jingui kidney qi tonifying tablets |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |