CN101514327B - Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase - Google Patents
Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase Download PDFInfo
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- CN101514327B CN101514327B CN2008100206352A CN200810020635A CN101514327B CN 101514327 B CN101514327 B CN 101514327B CN 2008100206352 A CN2008100206352 A CN 2008100206352A CN 200810020635 A CN200810020635 A CN 200810020635A CN 101514327 B CN101514327 B CN 101514327B
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Abstract
The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature amylase, which has the preservation number of CCTCC NO: M207193, and is obtained through compound mutation of the prior Bacillus licheniformis system, preservation, rejuvenation and a selective breeding method; and then the Bacillus licheniformis is optimized by a culture medium formulation and optimized by fermenting technological conditions so as to establish the method for producing the acid-proof high-temperature amylase, wherein the method comprises the following steps: air culturing the Bacillus licheniformis for about 72 hours at a temperature of 37 DEG C to reach an enzyme generating peak; and obtaining concentrated enzyme fluid through flocculating treatment, pressure filtration and ultra-filtration concentration or obtaining different amylase preparations such as micro pills, granules or powder through other post treatment modes. The produced high-temperature amylase has good acid-proof activity, and can be widely applied in the food and medicament industries, particularly the industries such as alcoholic fermentation, monosodium glutamate, antibiotics, textile and papermaking.
Description
Invention field
A kind of acidproof high-temperature amylase strain and the method for producing acidproof alpha-amylase thereof belong to technical field of bioengineering.
Background technology
Alpha-amylase is one of zymin kind of the most successful industrial applications, and this is because enzymically hydrolyse can be in the stability of controlling starch hydrolysis, atopic and product to a greater extent.Gentle reaction conditions comprises lower temperature of reaction, near neutral pH etc., reduce thus the energy in producing consumption, reduce production costs and environmental pollution, can improve output and bring multiple advantages such as useful economic benefit, social benefit.
Amylase has been widely used in industries such as processing, food service industry such as maltose, beer, yellow rice wine, glucose, monosodium glutamate, the microbiotic of destarch, the paper industry starch slurry of textile industry; In detergent industry, make an addition to proteolytic enzyme, lipase and to make multienzyme washing powder etc. in the washing powder, have utmost point purposes widely.
In the middle gentle alpha-amylase of commodity production at present, generally all effective about pH 4.9-7.5, after exceeding this scope, vigor descends very fast.In food service industry, industries such as textile industry and papermaking make diastatic efficient reduce because the pH in different fermentations stage changes.Be summarized as follows according to document and the patent reported at present:
Bacterial isolates B-9545, the peak enzymolysis-ability condition is: 50 ℃, pH 9.0 (Yan Haolin, Zhang Huili etc.＜alkali starch enzyme produces screening and the condition of enzyme production research of bacterium 〉, JOURNAL OF MICROBIOLOGY, 1996,1:26～30);
Bacillus subtilis 14140﹠amp; 14141, the peak enzymolysis-ability condition is: and 60～70 ℃, pH 6.0 (Guan Bin, thank lysol, Ding Youfang, grand speech spring, the seed selection of＜Bacillus subtillis A-Alpha-amylase high yielding strain〉Zhejiang Agricultural Univ's journal 1997,23 (5): 88～92);
Alkaline-resisting bud robe bacillus 9-A2, the peak enzymolysis-ability condition is: 50 ℃, pH 8.0 (Jia Shiru, Zhao Shuxin, Frznco GTevec, the separation and the screening of＜alkaline-resisting bud robe bacillus alkali starch enzyme research I-bacterial classification〉Tianjin institute of light and textile industries journal 1994,2:1～6);
Bud robe bacillus (Bacillus) ZX99, the peak enzymolysis-ability condition is: 60 ℃, pH 6.0 (Zhang Yingjiu, Zhu Xuejun, key, Li Jiping, Xue Yan, the evaluation of＜a kind of amylase and the screening of bacterium producing multi enzyme preparation thereof〉microbiology circular 2002,29 (5): 38～41);
Have a liking for alkali bud robe bacillus SX212, the peak enzymolysis-ability condition is: 55 ℃, pH 9.0 (Ma Xiaojun, Zhang Xiaojun, Yang Ling, Feng Qingping, the screening of＜Starch debranching enzyme generation bacterium and the research of fermentation condition〉Lanzhou University's journal (natural science edition) 2001,37 (6): 80～85);
Nocardia bacteria strain Nocarlda sp 82, the peak enzymolysis-ability condition is: 60 ℃, pH 7.0 (Wang Yinding, Yang Xiuqin, Wang Jianping, the separation and the evaluation of the outer beta-amylase of＜Nocardia bacteria born of the same parents〉University Of Hebei's journal (natural science edition) 1997,17 (2): 41～45);
Asp.oryzaevar.30103, the peak enzymolysis-ability condition is: 60 ℃, pH 5.0; Rhi.niveus 4810, and the peak enzymolysis-ability condition is: 60 ℃, pH 5.0 (Guan Bin, thank lysol, Ding Youfang, grand speech spring, the seed selection of＜Bacillus subtillis A-Alpha-amylase high yielding strain〉Zhejiang Agricultural Univ's journal 1997,23 (5): 88～92);
Lichens bud robe bacillus A.404, the peak enzymolysis-ability temperature is higher than 90 ℃; (the stupid person of outstanding talent of poplar, Hu Xuezhi, the A.4041 research of fire resistant alpha-diastase of＜ground farming bud robe bacillus〉dimension general information 1997:286～289);
Lichens bud robe bacillus (Bacillus licheni/ormis) Js-S, the peak enzymolysis-ability condition is: 90 ℃; (Li Youhong, Wu Yanyong,＜lichens bud robe bacillus JS-5 one diastatic research〉microorganism journal 1998,29 (4): 314～316);
Withered grass bud robe bacillus Bacillus subtilise 8631, its peak enzymolysis-ability condition is: 65C, pH5.6 (Jiang Yongming, history forever, Sui Dexin, the research II. of＜withered grass bud robe bacillus 86315 α-Dian Fenmei separates purification, character and kinetics〉Jiangsu Agricultural College's journal 1992,13 (2): 47～5);
Aspergillus oryzae (Aspergillus oryzae) mutant strain 6-193, its peak enzymolysis-ability condition is: 60 ℃, pH 5.0～6.0 (Kong Xianliang, the stupid English of king, Cui Yajie, ginger face duckweed, the purifying of＜aspergillus oryzae 6-193 α-Dian Fenmei and the research of character〉microorganism journal 1991,31 (4): 274～280);
Have a liking for alkali bud robe bacillus NT-3, its peak enzymolysis-ability condition is: 50 ℃, pH 9.0 (field groupuscule, the preliminary study of＜basophilia bud robe bacillus (Bacillus sp) NT-39 and the alkali starch enzyme that produces thereof〉Wuhan University's journal (natural science edition) 1992,2:105～111);
Have a liking for the α-Dian Fenmei that alkali Zymomonas mobilis (alkalomonas amylolytics) N-10 produces, its peak enzymolysis-ability condition is: 50 ℃, pH10 (Ma Yan and, Xue Yanfen, Dou Yuetan,＜a kind of alkali alpha amylase, its encoding gene and purposes〉patent publication No. CN1500870A);
Archeobacteria sesame field sulfuration leaf mattress B1, its peak enzymolysis-ability condition is: 60 ℃ of (Liu Li, Wu's flap, Chen Wei, Zhang Shuzheng, the optimization of the novel alpha-amylase gene engineering bacteria expression condition of＜sesame field sulfolobus solfataricus〉Chinese biological engineering magazine 2003,23 (1): 70～74);
Archeobacteria Pyrococcus furious, its peak enzymolysis-ability condition is: 95 ℃, pH 5.0 (Shen Wei, Wang Zhengxiang, the expression of＜archeobacteria pyrococcus furious thermophilic alpha-amylase gene in intestinal bacteria〉food and fermentation industries 2002,29 (3): 10～14);
Thermomucor indicae-seudaticae, the peak enzymolysis-ability condition is: 60 ℃, pH 7.0 (SanjeevKumar and T.Satyanarayana Purification and Kinetics of a Raw Starch-Hydrolyzing, Thermostab 1e, and Neutral Clucoamylase of the Thermophilic Mold Thermomucorindicae-seudaticae Biotechnol.Prog.2003,19,936～944);
Bacteroides amylophilus 70, the peak enzymolysis-ability condition is: 43 ℃ of pH 6.3 (Steven J.Mcwethy Paul A.Hartman Purification and Some Properties of an Ext-racellularAlpha-Amylase from Bacteroides amylophilusl Journal of Bacteriology, Mar.1977, p.1537～1544);
Thermomonospora curvata, the peak enzymolysis-ability condition is: 65 ℃, pH 6.0 (FredStutz-enberger Rick Carnel Amylase Production by Thermomonospora curvataApplied and Enyromental Microbiology, Aug.1977, p.234～236);
B.stearothermophilus1518, the peak enzymolysis-ability condition is: 60 ℃; B.coagulans 43P peak enzymolysis-ability condition is: 70 ℃; Bacillus stearothermophilus ATCC 7954, the peak enzymolysis-ability condition is: 90 ℃ of (Paul A.Hartman Ralph Wellerson, JR.P.A.Tetrault BacillusStearothermophilusI.-Thermal and pH Stability of the Amylase July 16,1954);
Bcillus subtilis strain W23﹠amp; B.Amyloliquefaciens strain F, the peak enzymolysis-ability condition is 65 ℃ of (N.E.WELTER ' AND L.LEON CAMPBELL Comparison of a Amylase ofthe α-Amylase of Bacillus subtilis and Bacillus amyloliquefaciens JOURNAL OFBACTERIOLOGY, Oct.1967, p.1131～1135);
Bacillus species NRRLB-3881, the peak enzymolysis-ability condition is 50 ℃, pH 9.5 (E.W.BO-YERAND M.B.INGLE Extracellular Alkaline Amylase from a Bacillus SpeciesJOURNAL OF BACTERIOLOGY, June 1972, p.992～1000);
Thermomonospora curvata, the peak enzymolysis-ability condition is 65 ℃, pH 6.0 (J.L.GLYMPHAND.J.STUTZENBERGER Production, Purification, and Characterizat-ion of α-Amylase from Thermomonospora curvata APPLIED AND ENVIRONMENTALMICROBIOLOGY, Oct.1977, P.391～397);
Halobacteriumhalobium, the peak enzymolysis-ability condition is 55 ℃, pH 6.6 (WENDYA.GOODAND PAUL A.HARTMAN Properties of the Amylase from Halobacteriumha-lobium JOURNAL OF BACTERIOLOGY, Oct.1970, p.601～603);
Utilize the genetically engineered of the amylase gene structure of bud robe bacillus NCIMB40916 to produce bacterium, the alkali starch enzyme peak enzymolysis-ability condition that produces is: 55 ℃, pH 9.5 (haler Ao Telapubijiaen R Nelson,＜alkaline bud pole bacterium amylase〉patent publication No. CN1399676A);
And effective bacterial classification of acidproof pH 3.8-6.5 and zymin are not appeared in the newspapers as yet.
Summary of the invention
The method that the purpose of this invention is to provide a kind of acidproof high-temperature amylase strain and produce acidproof alpha-amylase, by complex mutation to original Bacillus licheniformis system, preservation, rejuvenation, and seed selection, obtain a strain and produce the bacterium of acidproof alpha-amylase, pass through culture medium prescription optimization and optimization of fermentation condition again, set up the method for producing acidproof alpha-amylase, the alpha-amylase of producing has good acidproof activity.
Technical scheme of the present invention: the bacterium of acidproof alpha-amylase is produced in a strain, classification called after Bacillus licheniformis (Bacillus licheniformis) BL-mut 08, be preserved in Chinese typical culture collection center, deposit number is CCTCC NO:M 207193.
Utilize described bacterial classification to produce the method for acidproof alpha-amylase, adopting Bacillus licheniformis (Bacilluslicheniformis) BL-mut 08 is starting strain, and through seed culture, liquid fermentation and culture and aftertreatment obtain acidproof high-temperature starch enzyme solution later on,
Slant medium is counted with g/L: beef broth 3-6, peptone 5-15, yeast powder 1-5, NaCl 1-5, starch 5-15, glucose 20-80, agar 15-25; Culture temperature 30-40 ℃, incubation time 20-40h;
The shake-flask culture base is counted with g/L: soybean cake powder 20-50, cottonseed meal 20-50, (NH
4)
2SO
41-10, CaCl
20.2-1.0, corn steep liquor 15-40, lactose 100-200, Trisodium Citrate 1-10, dipotassium hydrogen phosphate 10-20, potassium primary phosphate 5-10; Culture temperature 30-42 ℃, incubation time 20-40h;
Seed culture: the seed tank culture base is counted with g/L: glucose 50-100, yeast powder 10-50, cottonseed meal 5-20, ammonium sulfate 2-6, Trisodium Citrate 2-10, CaCl
20.2-0.9, polyether antifoam agent 0.2-0.8, dipotassium hydrogen phosphate 4-10, potassium primary phosphate 1-10; Culture condition: seeding tank volume 500L, 60% charge amount; Leavening temperature 30-40 ℃; Ventilation 0.2-1.0vvm; Tank pressure: 0.2～1.0kg/cm
2Time 20-40h;
Liquid state fermentation is cultivated: fermentation tank culture medium is counted with g/L: glucose 50-100, yeast powder 10-50, cottonseed meal 5-20, ammonium sulfate 2-6, Trisodium Citrate 2-10, CaCl
20.2-0.9, corn steep liquor 15-50, polyether antifoam agent 0.2-0.8, dipotassium hydrogen phosphate 4-10, potassium primary phosphate 1-10; Culture condition: use 5 tons of fermentor tanks to ferment, 60% charge amount, leavening temperature 30-40 ℃, ventilation 0.2-1.0vvm, tank pressure 0.2～1.0kg/cm
2, time 40-80h.
Last handling process is: after the liquid state fermentation through flocculation, press filtration, the filtrate ultrafiltration and concentration is collected concentrated solution, is acidproof high-temperature starch enzyme solution;
(1) flocculation: add after the liquid state fermentation and put jar water of fermented liquid weight 35%-45%, add and put jar CaCl of fermented liquid weight 5%-10%
2Lime aqueous solution with NaOH solution that contains 12% mass concentration and 7% mass concentration is transferred pH 9.5-10.0, add cation polypropylene acyl ammonia, make that positively charged ion polyacrylamide concentration is 50mg/kg in the system, add and put jar dextrin of fermented liquid weight 1%-2%, be heated to 60 ℃, insulated and stirred 30min;
(2) press filtration: Plate Filtration;
(3) filtrate ultrafiltration and concentration: filtrate is that the tubular fibre tubular type ultra-filtration membrane of 10000Da concentrates with molecular weight cut-off, is concentrated into and puts 50% of jar fermented liquid weight, and after measured, enzyme is lived and is 10000-25000u/mL.Measuring method: the measuring method of the high temperature resistant alpha Amylase preparation of QB/T 2306-97.
Beneficial effect of the present invention: the invention discloses a kind of Bacillus licheniformis (Bacillus licheniformis) BL-mut 08 that produces acidproof alpha-amylase, deposit number is CCTCC NO.M207193, by to original Bacillus licheniformis system complex mutation, preservation, rejuvenation, and selection and obtaining, pass through culture medium prescription optimization and optimization of fermentation condition again, set up the method for producing acidproof alpha-amylase, in 37 ℃, the air cultivation reached in about 72 hours produces the enzyme peak, through flocculation treatment, press filtration, ultrafiltration and concentration obtains concentrating enzyme liquid or obtains different diastases by other aftertreatment processing modes, as micropill, and particle and pulvis.The alpha-amylase of producing has good acidproof activity, can be widely used in food, pharmaceutical industries, especially as industry such as zymamsis, monosodium glutamate, microbiotic and weaving, paper-making industry.
The biological material specimens preservation
Bacillus licheniformis (Bacillus licheniformis) BL-mut 08 has been preserved in Chinese typical culture collection center, and depositary institution is called for short CCTCC, and preservation date is on December 6th, 2007, and deposit number is CCTCC NO:M 207193.
Description of drawings
Relative enzyme (%) alive when the acidproof high-temperature starch enzyme product of Fig. 1 uses under the condition of different pH.
Embodiment
Below describe enforcement of the present invention in detail by specific embodiment, as to the qualification of the scope of the present invention.
Embodiment 1:
Inclined-plane prescription (calculating): beef broth 4, peptone 10, yeast powder 3, NaCl3, starch 10, glucose 50, agar 15-25 with g/L; 35 ℃ of culture temperature, incubation time: 30h;
Shake bottle prescription (calculating): soybean cake powder 40, cottonseed meal 30, (NH with g/L
4)
2 SO
45, CaCl
20.5, corn steep liquor 30, lactose 150, Trisodium Citrate 5, dipotassium hydrogen phosphate 15, potassium primary phosphate 7,40 ℃ of culture temperature, incubation time: 30h;
Seeding tank prescription (calculating): glucose 75, yeast powder 30, cottonseed meal 15, ammonium sulfate 5, Trisodium Citrate 8, CaCl with g/L
20.5, P.P.E. (polyether antifoam agent) 0.5, dipotassium hydrogen phosphate 7, potassium primary phosphate 7, leavening temperature: 35 ℃, ventilation: 0.8vvm, tank pressure: 0.8kg/cm
2, time: 36h, seeding tank volume: 500L, 60% charge amount.
Produce a jar prescription (calculating): glucose 75, yeast powder 30, cottonseed meal 15, ammonium sulfate 4, Trisodium Citrate 8, CaCl with g/L
20.7, corn steep liquor 40, P.P.E. (polyether antifoam agent) 0.6, dipotassium hydrogen phosphate 6, potassium primary phosphate 8, leavening temperature: 35 ℃, ventilation: 0.8vvm, tank pressure: 0.8kg/cm
2Time: 60h uses 5 tons of fermentor tanks to ferment 60% charge amount.
Add after the liquid state fermentation and put jar fermented liquid weight 40.5% water, add and put jar CaCl of fermented liquid weight 8%
2Lime aqueous solution with NaOH solution that contains 12% mass concentration and 7% mass concentration is transferred pH 9.85, add cation polypropylene acyl ammonia, make that positively charged ion polyacrylamide concentration is 50mg/kg in the system, add and put jar dextrin of fermented liquid weight 1.5%, be heated to 60 ℃, insulated and stirred 30min, Plate Filtration then, filtrate is that the tubular fibre tubular type ultra-filtration membrane of 10000Da concentrates with molecular weight cut-off, is concentrated into and puts about 50% of jar fermented liquid weight, after measured, enzyme is lived and is 19553U/mL, measures foundation: the measuring method of the high temperature resistant alpha Amylase preparation of QB/T 2306-97.
Claims (1)
1. the bacterium of acidproof alpha-amylase is produced in a strain, and classification called after Bacillus licheniformis (Bacilluslicheniformis) BL-mut 08 has been preserved in Chinese typical culture collection center, and deposit number is CCTCC NO:M 207193.
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| CN102409006B (en) * | 2011-06-29 | 2014-05-28 | 湖南农业大学 | Strain and process method for producing acidic thermophilic amylase |
| CN102618518A (en) * | 2012-03-22 | 2012-08-01 | 无锡德冠生物科技有限公司 | Wide-temperature amylase production method |
| CN112391324B (en) * | 2020-12-09 | 2022-04-08 | 山东隆科特酶制剂有限公司 | Strain for producing acid-resistant high-temperature alpha-amylase and application thereof |
| CN112522239B (en) * | 2020-12-09 | 2022-04-22 | 山东隆科特酶制剂有限公司 | Acid-resistant high-temperature alpha-amylase and production method thereof |
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| CN1702173A (en) * | 2005-06-27 | 2005-11-30 | 江南大学 | Coded high temperature acid alpha-amylase gene and its synthesis method, cloning and expression |
| CN1746301A (en) * | 2005-06-22 | 2006-03-15 | 天津科技大学 | Acid-proof and high-temperature resistant alpha-amylase and production thereof |
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| CN1746301A (en) * | 2005-06-22 | 2006-03-15 | 天津科技大学 | Acid-proof and high-temperature resistant alpha-amylase and production thereof |
| CN1702173A (en) * | 2005-06-27 | 2005-11-30 | 江南大学 | Coded high temperature acid alpha-amylase gene and its synthesis method, cloning and expression |
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