CN101513436A - Szechuan lovage rhizome-angelica total extract, preparation and application - Google Patents
Szechuan lovage rhizome-angelica total extract, preparation and application Download PDFInfo
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- CN101513436A CN101513436A CNA2008100578834A CN200810057883A CN101513436A CN 101513436 A CN101513436 A CN 101513436A CN A2008100578834 A CNA2008100578834 A CN A2008100578834A CN 200810057883 A CN200810057883 A CN 200810057883A CN 101513436 A CN101513436 A CN 101513436A
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Abstract
The invention relates to a total extract extracted from compound Szechuan lovage rhizome and angelica, wherein the total extract comprises 10 to 90 percent of phthalides-containing component and 5 to 40 percent of unsaturated fatty acid component. The invention also relates to a method for extracting the total extract, a composition containing the total extract, a method for preparing a preparation, and application of the total extract in preparing a medicament for treating gynecological diseases and diseases such as headache, arthralgia and the like.
Description
Technical field
The invention belongs to the modern Chinese medicine field, particularly, the present invention relates to the modern Chinese medicine that series is used to prepare diseases such as treatment gynaecopathia and headache, arthralgia pain.
Background of invention
The compound recipe rhizome of chuanxiong is returned by famous Chinese medicine Rhizoma Chuanxiong, when being grouped into, and has another name called foshousan, is that classical Chinese medicine medicine is right.We come from " general Ji ability side " at first and cure mainly pregnant damaging fetus, difficult labour, gynaecopathias such as retention of placenta, Golden Mirror of Medicine has been made more detailed dialectical analysis to its mechanism of curing the disease: ' the Fructus Citri Sarcodactylis person is said in name, and the meaning married woman is through all diseases in preceding, puerperal, also wonderful as Fructus Citri Sarcodactylis.Rhizoma Chuanxiong, when the principal agent that is classified as blood system, warm in nature and sweet in the mouth, suffering, with temperature can and blood, sweet can enriching blood, the hot blood that looses goes out also." this prescription is widely used in the disease of dialectical associated with it in afterwards the clinical practice, as menoxenia, abdominal pain during menstruation, married woman's difficult labour, postnatal blood stasis stomachache; Skin infection swells and ache; Rheumatic arthralgia; Diseases such as blood deficiency, blood stasis headache.
The research that the compound recipe rhizome of chuanxiong is returned is except traditional frying in shallow oil, boil, endure the administration technology, and modern research method comprises: ether extraction etc. behind ether extraction and the ethanol percolation, and these methods exist process cycle long, with an organic solvent wait defective in a large number.For example, ethanol percolation needs about 12 hours; In a large number with an organic solvent, go out danger in the operation easily, suitability for industrialized production acquires a certain degree of difficulty; Organic solvent might be removed unonly in the leaching process, and residue is arranged in the medicine; The use amount of reagent is big, and cost is higher; Technical process is long, and variable factor is many, is unfavorable for defectives such as product monitoring.
Supercritical extraction technique is a kind of novel extractive technique, has wide application in a plurality of fields.Supercritical fluid (SCF) refers to thermodynamic state to be in critical point, and (this moment, fluid was in a kind of special state between gaseous state and the liquid state, had very unique physicochemical properties for Pc, the Tc) fluid on.The viscosity of SCF approaches gas, and density approaches liquid, and diffusion coefficient has the advantage of gas and liquid concurrently between gas and liquid, both as the easy diffusion of gas, as liquid very strong solvability is arranged again.Thereby SCF has high diffusibility and highly dissoluble.Under the identical situation of other conditionally complete, density of liquid has been reacted its solvability to a great extent, and the density of SCF is relevant with pressure and temperature, and along with the increase of pressure, dielectric constant and density increase, and SCF increases the solvability of material.Supercritical extraction is exactly a minor variations of utilizing near SCF system temperature and pressure critical point, make the emergent properties of substance dissolves degree generation several magnitude realize its to the extraction of some component with separate.By changing the character that pressure or temperature change SCF, reach the purpose of optionally extracting various types of chemical compounds.Supercritical extraction technique mainly contains two class extraction processes: constant temperature blood pressure lowering process and constant voltage temperature-rise period.Difference is that the former separates SCF with solute after reducing pressure, the latter is that SCF realizes solute and separated from solvent through heating.Solvent can recycle repeatedly.The solvent that is commonly used for SCF has CO
2, NH
3, toluene etc.CO wherein
2Be industrial the most frequently used extractant, be characterized in: a) critical temperature low (31.06 ℃), extraction can be carried out under near the temperate condition the room temperature, and volatile components or biological active substances are seldom destroyed, and is suitable for the extraction of active skull cap components; B) critical pressure moderate (7.14MPa), operating condition is easy to reach, and at room temperature liquefaction pressure is 4Mpa-6MPa, is convenient to accumulating; C) safety non-toxic especially be fit to pharmacy, food industry, and extract and separate is once finished no solvent residue; D) it is not flammable to have a chemical inertness, and handling safety is cheap and easy to get; E) CO
2Can be recycled.
Supercritical extraction has manifested great vitality in modern Chinese traditional medicine field, and part Chinese medicine has been realized industrialization whereby.In different pressure, temperature range, carbon dioxide has different specific aims to the selection of material, can make Chinese medicine extraction purity higher, produce more efficient.In addition, adopt supercritical technology extraction effective ingredient, the oxygen consumption microorganism in the extract will be killed fully, and bioactive substance can be not destroyed.
We adopt supercritical extraction technique to extract a kind of total extract from the compound rhizome of Sichuan lovage Radix Angelicae Sinensis on the basis of fully research, and the effective ingredient of Rhizoma Chuanxiong and Radix Angelicae Sinensis is extracted.Simultaneously extract is carried out intensive pharmacological research, found that this extract has the advantage that identical indication other treatment medicine can't be reached, can develop the modern Chinese medicine of diseases such as being used to prepare treatment gynaecopathia and headache, arthralgia pain.
Summary of the invention
The purpose of this invention is to provide a kind of compound recipe rhizome of chuanxiong and sum up extract, wherein contain phthalide-type composition 20-95%, unsaturated fatty acid composition 5-40%.
Another object of the present invention provides a kind of method that compound recipe rhizome of chuanxiong of the present invention is summed up extract for preparing.
Another object of the present invention provides and contains the pharmaceutical preparation that compound recipe rhizome of chuanxiong of the present invention is summed up extract.
Another object of the present invention has provided compound recipe rhizome of chuanxiong of the present invention and has summed up the traditional Chinese compound medicine that extract is used to prepare diseases such as treatment headache, rheumatic arthralgia and gynecological's dysmenorrhea, menoxenia.
Detailed Description Of The Invention
Compound recipe rhizome of chuanxiong provided by the invention is summed up extract, wherein contains phthalide-type composition 20-95%, unsaturated fatty acid composition 5-40%.In the phthalide-type composition, Ligustilide content is 10-90%, preferred 20-80%, more preferably 30-70%, most preferably preferred 40-60%; The unsaturated fatty acid composition comprises linoleic acid, oleic acid or linolenic acid etc.
The concrete grammar that compound recipe rhizome of chuanxiong provided by the invention is summed up extract comprises the steps:
A. respectively Radix Angelicae Sinensis and rhizome of chuanxiong are returned pulverizing, cross 20 mesh sieves;
B. be 20-80% with weight ratio, preferred 30-70%, more preferably the Radix Angelicae Sinensis of 40-60% and weight ratio are 20-80%, and be preferred 3070%, more preferably the rhizome of chuanxiong of 40-60% returns powder to put supercritical CO
2The extraction apparatus extraction, extraction conditions is a pressure: 10-100Mpa, preferred 20-50Mpa; Temperature: 0-100 ℃, preferred room temperature-80 ℃; Get the thing separation condition: first separating still pressure: the 5-50Mpa, preferred 8-15Mpa, temperature: 0-100 ℃, preferred, 20-80 ℃; Second separating still pressure: the 1-20Mpa, preferred 4-6Mpa, temperature: 0-100 ℃, preferred 20-80 ℃;
C. collect the second separating still extract, filtration, centrifugal is got oil reservoir and is got total extract.
Compared with prior art, the preparation method of total extract of the present invention has plurality of advantages:
1. Technology advanced person, production cost is low, is fit to modernization industry and produces;
2. total extract that obtains and preparation onset thereof are rapid, good effect, no obvious toxic-side effects;
3. use supercritical CO
2Abstraction technique extracts medicine, does not use organic solvents such as ether and ethanol, product safety, natural;
4. abstraction technique speed of the present invention is fast, can finish one batch of product in the working day, shortens the production cycle greatly;
5. owing to easily react under the active component ligustilide high temperature in the Rhizoma Chuanxiong, Radix Angelicae Sinensis, and supercritical CO
2The abstraction technique extraction temperature is low, is suitable for the extraction to this active component;
6. supercritical CO
2Abstraction technique yield height, spe medium can be recycled, and can reduce production costs and the medical material consumption.
The inventor makes further research total extract, extract is made various pharmaceutical compositions, comprise tablet, pill, drop pill, powder agent, lozenge, capsule, soft capsule, gel, syrup, unguentum, suspension, cachet and suppository etc., particularly drop pill and capsule etc.Concrete preparation technology is as follows:
Drop pill preparation technology is as follows:
A. get above-mentioned rhizome of chuanxiong and sum up extract, with the adjuvant heating and meltings such as Polyethylene Glycol of conventional part by weight, heating-up temperature is 60-90 ℃, and wherein Polyethylene Glycol can use a kind of in Macrogol 4000-Macrogol 2000 0 series or their mixture;
B. put in the drop pill machine, 60-90 ℃ of constant temperature, the control flow velocity splashes in the dimethicone condensed fluid condition: drip diameter: 2mm-5mm; Drip distance: 2-5cm; Drip speed: 30-60 drip/minute; Condensate temperature: 0 ℃-30 ℃; Condensed fluid height: 30-60cm;
C. get drop pill, wipe surface condensation liquid away, promptly get drop pill.
Soft capsule preparation technology is as follows:
A. the configuration of glue:
Ratio takes by weighing gelatin, glycerol, water routinely, places the glue bucket, 40-80 ℃ of water-bath glue, be molten state after, stir and to add opacifier titanium dioxide, toner cocoa cotton etc., mix homogeneously down;
B. the glue evacuation of handling well is removed bubble;
C. glue and the total extract of handling well of the present invention added in capsule machine glue bucket and the hopper compacting respectively.Soft gelatin capsule was put to the high and dry chamber dry 24 hours, got qualified glue nine promptly.
The total extract that the inventive method obtains is a main active with phthalide-type composition and unsaturated fatty acids composition, wherein the phthalide-type component content can reach 10-90%, wherein ligustilide content is 10-90% in the phthalide-type composition, the unsaturated fatty acid composition can reach 1-40%, preferred 5-40%, wherein unsaturated fatty acid comprises linoleic acid, oleic acid, linolenic acid etc.
Extraction process of the present invention can make rhizome of chuanxiong sum up that phthalide-type composition and unsaturated fatty acids composition keep certain proportionate relationship in the extract, and the two proportionate relationship is vital to the medicinal effects of total extract.This be because: 1. the phthalide-type composition has the effect of tangible blood circulation promoting and blood stasis dispelling, anti-inflammatory and antalgic, but also has than significant side effects, may certain infringement be arranged to gastrointestinal tract, kidney and nervus centralis; Therefore should note suitably reducing the ratio of this constituents in medicine; And carry out compatibility and reduce toxicity; 2. unsaturated fatty acid has certain cytoprotection, particularly in gastrointestinal tract, can prevent or reduce the untoward reaction that other constituents causes, unsaturated fatty acid also has certain antiinflammatory and the effect that improves hemorheology in addition.Therefore, we are by studying for a long period of time, and the technology of finding out above-mentioned preparation total extract combines above two constituents, plays the effect of potentiation, attenuation, and these characteristics have obtained further proof in the test that we carried out.
The inventor has carried out further pharmacodynamics, pharmacology and toxicologic study with total extract, comprise rat and rabbit are tested the general pharmacology test of mice and dog etc. the influence test in body uterus, the anxious malicious of the long term toxication, rat and the mice that influence test, blood coagulation resisting function test, antiplatelet aggregative activity test, antiinflammatory test, analgesic test, rat and dog of antagonism oxytocin.From above test, find rhizome of chuanxiong sum up extract have spasmolytic, ease pain, invigorate blood circulation, pharmacologically active effect such as antiinflammatory, normal physical signs is not had influence, toxicity is low, characteristics such as safety height possess the condition that exploitation becomes new Chinese medicine.Total extract is made drop pill and soft capsule, and this dosage form helps this effective ingredient and absorbs fully, fast in human body, gives full play to its drug action, can be used for treating diseases such as headache, rheumatic arthralgia and gynecological's dysmenorrhea, menoxenia clinically.
The present invention merges the effective ingredient of Rhizoma Chuanxiong, Radix Angelicae Sinensis in this compound recipe, the drug action of proof united extraction thing is better than the independent extract of Radix Angelicae Sinensis in the relevant dysmenorrhea pharmacological testing that is carried out, rhizome of chuanxiong sum up extract onset time very fast, drug action is strong, effective dose is little; Though find that in anticoagulation experiment Radix Angelicae Sinensis extract blood coagulation speed and intensity have certain drug action, and not obvious, and rhizome of chuanxiong is summed up extract tangible blood coagulation resisting function is arranged; Rhizome of chuanxiong is summed up extract in the antiplatelet aggregation experiment tangible antiplatelet aggregative activity, and Radix Angelicae Sinensis extract does not show this effect.
Further specify the present invention below by embodiment.It should be understood that embodiments of the invention are to be used to illustrate the present invention rather than limitation of the present invention.Essence according to the present invention all belongs to the scope of protection of present invention to the simple modifications that the present invention carries out.Except as otherwise noted, the percent among the present invention is percetage by weight.
The preparation that embodiment 1 rhizome of chuanxiong is summed up extract
Get Rhizoma Chuanxiong, Radix Angelicae Sinensis medical material, to account for the Rhizoma Chuanxiong of medical material weight 30% and account for medical material weight 70% Radix Angelicae Sinensis medical material or decoction pieces is crushed to 20 orders, put in the supercritical extraction device, extracted extracting pressure 30Mpa, 40 ℃ of temperature 3 hours, the first separating still pressure 12Mpa, 40 ℃ of temperature, the second separating still pressure 5Mpa, 40 ℃ of temperature.Get the second separating still liquid, sucking filtration, centrifugal, get oil reservoir promptly.Experimental result: sample yield 2.87, phthalide-type content 38.4%, ligustilide content 16.9%, linoleic acid content 3.21%.
Embodiment 2 rhizomes of chuanxiong are returned the preparation of drop pill
Get the total extract of embodiment 1,, stir, in the impouring drop pill machine with the Macrogol 4000 heating and melting, splash under 80 ℃ of constant temperature in 5-8 ℃ of methyl-silicone oil 50 condensed fluid, drip a fast 30-40 drip/minute, drip apart from 4cm, leach drop pill, clean silicone oil, packing is made drop pill promptly.The result shows: drop pill rounding, industrial easy to operate, physical and chemical index is qualified.
Embodiment 3 rhizomes of chuanxiong are returned preparation of soft capsule
Get the total extract of embodiment 1, take by weighing in proportion in gelatin, glycerol, putting of the water glue jar, 65 ℃ of water-bath glue, be molten state after, stir and to add the toner cacao brown down.To be mixed evenly after, evacuation removes bubble.Glue and the medicinal liquid handled well are added in capsule machine glue bucket and the hopper compacting respectively.Soft gelatin capsule was put to the hothouse dry 24 hours, got qualified soft gelatin capsule, promptly.
Embodiment 4 rhizomes of chuanxiong are summed up the influence development test of extract to oxytocin induced mice dysmenorrhea
Experiment purpose
Observe rhizome of chuanxiong and sum up the influence of extract, and relatively rhizome of chuanxiong is summed up the two drug action and each self-applying characteristic of extract and single Radix Angelicae Sinensis extract oxytocin induced mice dysmenorrhea.
Experiment material
1. animal subject
The ICR mice, female, body weight 20 ± 2g is provided by Beijing Vital River Experimental Animals Technology Co., Ltd., the quality certification number: SCXK11-00-0008.
2. be subjected to the reagent thing
Rhizome of chuanxiong is summed up extract: lot number is provided by the Beijing Ruikang Pharmaceutical Co., Ltd: 020112, be made into emulsion suspension liquid for the animal gastric infusion with distilled water and tween 80.
Radix Angelicae Sinensis extract: provide by the Beijing Ruikang Pharmaceutical Co., Ltd, be made into emulsion suspension liquid for the animal gastric infusion with distilled water, tween.
3. reagent and instrument
Oxytocin inj: 10u/ml, Nanjing new day bioid length of schooling medicine company limited is produced lot number: 020301.Diethylstilbestrol injection: 2mg/ml, Mingxing Pharmaceutical Factory, Guangzhou production, lot number: 001001.
Experimental result and method
1. grouping
96 of mices are divided into eight groups at random, i.e. matched group, rhizome of chuanxiong are summed up extract 60mg/kg, 30mg/kg, 15mg/kg group, Radix Angelicae Sinensis extract 60mg/kg, 30mg/kg, 15mg/kg group.
2. route of administration and administration time
Irritate stomach and be subjected to the reagent thing, model group gives the tween 80 aqueous solution of equivalent, be administered five times, once a day, formal experiment in a hour after the last administration.The 3rd day only begins lumbar injection diethylstilbestrol injection 0.2ml/ after the administration, and continuous three times, once a day.
3. experimental technique
After the last administration one hour, mouse peritoneal injection oxytocin inj 2u/ only, the record mice was turned round the number of times of body in 10 minutes.
4. statistical method:
Each administration group all compares with matched group, calculates every index and organizes a t check, and P＜0.05 has been considered to significant difference.
5. result
The results are shown in Table 1.
The influence that table 1 two medicines stimulate the menstrual flow to the oxytocin induced mice
Annotate: * * compares P＜0.01 with model group
6. conclusion
Above result shows that rhizome of chuanxiong is summed up extract 60mg/kg, and 30mg/kg and Radix Angelicae Sinensis extract 60mg/kg all can obviously alleviate the mouse writhing number of times, and statistical procedures points out two medicines can resist the hysterospasm pain that oxytocin causes.Compare two medicines, rhizome of chuanxiong is summed up the extract action intensity, and effect dosage also is better than Radix Angelicae Sinensis extract.
Embodiment 5 rhizomes of chuanxiong are summed up the research of extract blood coagulation resisting function
Experiment purpose
Observe rhizome of chuanxiong and sum up the blood coagulation resisting function of extract, and relatively the compound recipe rhizome of chuanxiong is summed up the two the drug action of extract and single Radix Angelicae Sinensis extract rat.
Experiment material
1. animal subject
The Wistar rat, body weight 190 ± 20g purchases the animal center in Beijing Medical University, animal card number: the moving word 01-3056 of doctor, raising place is this institute animal feeding center.
2. be subjected to the reagent thing
Rhizome of chuanxiong is summed up extract: provided by the Beijing Ruikang Pharmaceutical Co., Ltd, lot number 020108 is made into emulsion suspension liquid for the animal gastric infusion with distilled water and soil temperature-80.
Radix Angelicae Sinensis extract: provide by the Beijing Ruikang Pharmaceutical Co., Ltd, be made into emulsion suspension liquid for the animal gastric infusion with distilled water and soil temperature-80.
3. reagent and instrument
The rotary flow graph of HAAKE-CV100/RV Germany, LXT-II centrifuge, KH-120A high speed hematocrit microcentrifuge Japan, LXT-2 centrifuge Shanghai medical apparatus are analyzed factory
Experimental technique and result
1. grouping
70 of rats are divided at random: blank group, rhizome of chuanxiong sum up extract big (36mg/kg), in (18mg/kg), little (9mg/kg) dosage group, Radix Angelicae Sinensis extract big (36mg/kg), little (18mg/kg) dosage group, 10 of every treated animals.
2. route of administration and administration time
Every day, oral administration was 1 time, and volume is 10ml/kg.Continuous irrigation stomach 7 days, matched group is given isometric soil temperature-80 aqueous solution.
3. experimental technique
Behind the successive administration 7 days, use 3% pentobarbital sodium with rat anesthesia, blood is got at the place by the animal ventral aorta.To getting blood with sodium citrate anticoagulation (the two volume ratio is 1: 9), bathe 1min with 37 ℃ of temperature of 500ml whole blood, add 1.29% calcium chloride, 100 μ l and carry out multiple calcium, and the multiple calcium index of mensuration blood coagulation, and can obtain following three parameters simultaneously: clotting time (tr), dynamic blood coagulation speed (d τ/dt), blood coagulation intensity (τ m).
4. statistical method
Each administration group all compares with matched group, calculates every index and organizes a t check, and P＜0.05 has been considered to significant difference.
5. result
The results are shown in Table 1.
Table 2 rhizome of chuanxiong is summed up extract to the anticoagulant effect research of rat
* with blank group t check P＜0.05
6. conclusion
Above result shows, rhizome of chuanxiong is summed up the big or middle dosage group of extract and is all shown the obvious suppression effect for clotting time, speed two aspect indexs, heavy dose of group still has obvious reduction effect to blood coagulation intensity simultaneously, though middle dosage group descends meansigma methods, does not reach remarkable limit.The heavy dose of group of Radix Angelicae Sinensis extract has the obvious suppression effect to clotting time, though speed and intensity there are certain drug action, and not obvious.In sum, rhizome of chuanxiong is summed up extract tangible blood coagulation resisting function, and is better than Radix Angelicae Sinensis and uses separately.
Embodiment 6 rhizomes of chuanxiong are summed up the extract study on antiplatelet aggregation effect
Experiment purpose
Observe rhizome of chuanxiong and sum up the influence of extract rat platelet aggregation and whole blood viscosity, plasma viscosity, and the rhizome of chuanxiong anti-aggregation of summing up extract and (same process extraction) Radix Angelicae Sinensis extract relatively.
Experiment material
1, animal subject Wistar rat, body weight 190 ± 20g purchases the animal center in Beijing Medical University, animal card number: the moving word 01-3056 of doctor, raising place is this institute animal feeding center.
2. be subjected to the reagent thing: rhizome of chuanxiong is summed up extract: lot number is provided by the Beijing Ruikang Pharmaceutical Co., Ltd: 020108, be made into emulsion suspension liquid for the animal gastric infusion with distilled water and soil temperature-80.
Radix Angelicae Sinensis extract: provide by the Beijing Ruikang Pharmaceutical Co., Ltd, be made into emulsion suspension liquid for the animal gastric infusion with distilled water and soil temperature-80.
3. reagent and instrument
The rotary flow graph of HAAKE-CV100/RV Germany
DIC-3210 four road platelet aggregation instrument Japan
The LXT-II centrifuge
KH-120A high speed hematocrit microcentrifuge Japan
LBY-N6A automatic cleaning type rotating cylinder viscometer Beijing Puli gives birth to company
LBY-NW1 automatic cleaning type capillary viscosimeter Beijing Puli gives birth to company
Experimental result and method
1. grouping
60 of rats are divided at random: blank group, rhizome of chuanxiong sum up extract big (36mg/kg), in (18mg/kg), little (9mg/kg) dosage group, Radix Angelicae Sinensis extract big (36mg/kg), 10 of every treated animals.
2. route of administration and administration time
Every day, oral administration was 1 time, and volume is 10mL/kg.Continuous irrigation stomach 7 days, matched group is given isometric tween 80 aqueous solution.
3. experimental technique
3.1 behind the successive administration 7 days, use 3% pentobarbital sodium with rat anesthesia, blood is got at the place by the animal ventral aorta.To getting blood with citric acid anticoagulation (the two volume ratio is 1: 9), with 1000 rev/mins centrifugal 10 minutes, preparation becomes platelet rich plasma (PRP); Again with 3000 rev/mins centrifugal 15 minutes, be prepared as platelet poor plasma (PPP).The PRP and the PPP that prepare are mixed in proportion.Transfer transmittance about 4000 with platelet aggregation instrument, promptly PC is 3.0 * 10
5Ml, 36 ℃ of temperature were bathed 2 minutes, measured by the maximum percentage rate of assembling of the inductive platelet of ADP.
3.2 get the 1mL whole blood in addition, place the rotary viscosity design determining cup, bathe 1min in 37 ℃ of temperature, measure the whole blood viscosity under the different shear rates immediately.
3.3 platelet poor plasma (PPP) the 200 μ l that prepare when measuring platelet suck in the capillary viscosimeter, the plasma viscosity under measuring 37 ℃.
4. statistical method
Rhizome of chuanxiong is summed up each administration group of extract and is all compared with matched group, carries out the t check, and P＜0.05 has been considered to significant difference.
5. result
Table 1 rhizome of chuanxiong is summed up the effect research (number of animals n=10) of extract to rat platelet aggregation
* with blank group t check P＜0.05
6. conclusion
Above result shows, the rhizome of chuanxiong of three dosage groups sum up extract and Radix Angelicae Sinensis extract administration in 5 days to the hemorheology index of normal rat all less than influence, promptly do not produce interference for the normal blood flow variable element, make it to continue to keep normal.The rhizome of chuanxiong of dosage group big or middle is summed up extract and is all shown the obvious suppression effect for platelet aggregation, assembles meansigma methods and is reduced in about 20%, says that from the range of decrease its inhibitory action is relatively gentleer; Radix Angelicae Sinensis extract does not show the effect of antiplatelet aggregation, shows with regard to antiplatelet aggregation rhizome of chuanxiong reversion side drug effect outline to be better than Radix Angelicae Sinensis unit's medicine.
Claims (10)
1. a compound recipe rhizome of chuanxiong is summed up extract, wherein contains phthalide-type composition 20-95%, unsaturated fatty acid composition 5-40%.
2. the described total extract of claim, wherein in the phthalide-type composition, ligustilide content is 10-90%.
3. the described total extract of claim, wherein in the phthalide-type composition, ligustilide content is 20-80%.
4. the described total extract of claim, wherein in the phthalide-type composition, ligustilide content is 30-70%.
5. the described total extract of claim, wherein in the phthalide-type composition, ligustilide content is 40-60%.
6. method for preparing the extract of claim 1-5 comprises:
A. respectively Radix Angelicae Sinensis and rhizome of chuanxiong are returned pulverizing, cross 20 mesh sieves;
B. be that 20-80% Radix Angelicae Sinensis and weight ratio are that the rhizome of chuanxiong of 20-80% returns powder to put supercritical CO with weight ratio
2The extraction apparatus extraction, extraction conditions is a pressure: 10-100Mpa; Temperature: 0-100 ℃; Get the thing separation condition: first separating still pressure: the 5-50Mpa, temperature: 0-100 ℃; Second separating still pressure: the 1-20Mpa, temperature: 0-100 ℃;
C. collect the second separating still extract, filtration, centrifugal is got oil reservoir and is got total extract.
7. pharmaceutical composition comprises that the described rhizome of chuanxiong of each claim of claim 1-5 sums up extract and adjuvant.
8. the compositions of claim 7 is prepared as tablet, pill, drop pill, the agent of powder art, lozenge, capsule, soft capsule, gel, syrup, unguentum, suspension, cachet and suppository.
9. the compositions of claim 7-8 is prepared as drop pill and soft capsule.
10. the described rhizome of chuanxiong of each claim of claim 1-5 and 7-9 is summed up the traditional Chinese compound medicine that extract or compositions are used to prepare diseases such as treatment headache, rheumatic arthralgia and gynecological's dysmenorrhea, menoxenia.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102861148A (en) * | 2012-10-19 | 2013-01-09 | 天津中医药大学 | Chinese medicine preparation for treating primary dysmenorrheal and preparation method and application |
| CN103784881A (en) * | 2014-02-18 | 2014-05-14 | 北京创立科创医药技术开发有限公司 | Traditional Chinese medicinal composition for treating dysmenorrhea and preparation method thereof |
| CN114377057A (en) * | 2022-02-11 | 2022-04-22 | 上海乾原健企业发展集团有限公司 | Preparation method of wax therapy patch |
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2008
- 2008-02-20 CN CNA2008100578834A patent/CN101513436A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102861148A (en) * | 2012-10-19 | 2013-01-09 | 天津中医药大学 | Chinese medicine preparation for treating primary dysmenorrheal and preparation method and application |
| CN102861148B (en) * | 2012-10-19 | 2014-04-02 | 天津中医药大学 | Chinese medicine preparation for treating primary dysmenorrheal and preparation method and application |
| CN103784881A (en) * | 2014-02-18 | 2014-05-14 | 北京创立科创医药技术开发有限公司 | Traditional Chinese medicinal composition for treating dysmenorrhea and preparation method thereof |
| CN103784881B (en) * | 2014-02-18 | 2018-07-20 | 北京创立科创医药技术开发有限公司 | A kind of Chinese medicine composition and preparation method thereof for treating dysmenorrhoea |
| CN114377057A (en) * | 2022-02-11 | 2022-04-22 | 上海乾原健企业发展集团有限公司 | Preparation method of wax therapy patch |
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