CN101508708A - Method for purifying concentrated stevioside by using nanofiltration - Google Patents
Method for purifying concentrated stevioside by using nanofiltration Download PDFInfo
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- CN101508708A CN101508708A CNA200910115114XA CN200910115114A CN101508708A CN 101508708 A CN101508708 A CN 101508708A CN A200910115114X A CNA200910115114X A CN A200910115114XA CN 200910115114 A CN200910115114 A CN 200910115114A CN 101508708 A CN101508708 A CN 101508708A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for purifying concentrated stevioside by a nanofiltration technology. A stevioside product is obtained by the following procedures: stevia leaf pretreatment, desalinization, adsorption, dialysis, bleaching, ion exchange, vaporization for removing alcohol, nanofiltration, steam sterilization and filtration, spray drying and the like. The method can help eliminate organic substances and microbes with relative molecular weight lower than 600 during the nanofiltration process, and precipitate inorganic salt and combines concentration, dialysis and sterilization. The method can help greatly improve product purity and reach the stevioside content up to 90% and the specific absorbance not greater than 0.015, and has the advantages of convenient operation, low operating pressure, energy saving, no pollution and the like.
Description
Affiliated technical field
The present invention relates to a kind of purification concentration method of Steviosides, especially utilize the method for nanofiltration purifying concentrated stevioside.
Background technology
Produce Steviosides at present and mainly contain two kinds of concentration technologies: a kind of is that decompression or normal pressure concentrate, and another kind is an evaporation concentration.Because the Steviosides molecule has better heat-resisting and stable properties, in being 4-10 solution, pH is heated to 50-100 ℃ as Steviosides, and all do not change from 0.5-24h heat-up time.A lot of producers generally all adopt normal pressure to concentrate or concentrating under reduced pressure when selecting concentration technology, and the result is because the cycles of concentration height, cause that the ratio absorbancy of product obviously exceeds standard, purity is low excessively.Evaporation concentration is that dilute solution is heated to boiling, makes wherein most solvent vaporizations, thereby reaches concentrated.Therefore also determined this technology to have some drawbacks.Increase as the concentration that can only make liquid glucose, can not reduce foreign matter content and the purity that improves glucoside in the glucoside.In addition, the evaporator wall local superheating makes some lower boiling organism produce carbonization, coking phenomenon.Also have the small part organism at normal temperatures or in aqueous ethanolic solution, be colourless state, but by long high temperature (more than 1 hour) and alcoholic acid volatilization, progressively be converted into coloring matter, cause the transmittance of product and raise the product purity reduction than absorbancy.And a large amount of energy of evaporation concentration need, also can cause the pollution of atmosphere and environment simultaneously.
Summary of the invention
For overcoming above-mentioned deficiency, the invention provides a kind of method of utilizing the nanofiltration purifying concentrated stevioside.
The technical solution adopted for the present invention to solve the technical problems is: described method comprises following operation in regular turn:
(a) pre-treatment: Folium Chrysanthemi is mixed with 50 ℃ of-55 ℃ of soft water, and soak and make the Steviosides extracting solution, through flocculation sediment and filter press two procedures, the filtrate behind the Plate Filtration is advanced next step operation;
(b) desalination: with the desalination of above-mentioned filtrate spent ion exchange resin, get the desalination filtrate of transmittance 〉=65%, specific conductivity≤6700us/cm, desalination filtrate is advanced next step operation;
(c) absorption: adopt Steviosides effective constituent in the above-mentioned desalination filtrate of resin absorption, treated back up to standard discharging of absorption waste liquid or reuse;
(d) resolve: the resin that has adsorbed Steviosides effective constituent with sodium hydroxide, salt acid treatment, with aqueous ethanolic solution the Steviosides effective constituent that is adsorbed on the resin is resolved again, must be than the desorbed solution of absorbancy≤0.65, desorbed solution advances next step operation;
(e) decolouring: gac inserted in the desorbed solution to mix stir, filter than the destainer of absorbancy≤0.16;
(f) ion-exchange: destainer is successively by after Zeo-karb and the anionite-exchange resin removal of impurities, and evaporation goes purely must remove pure liquid, goes pure liquid to advance next step operation;
(g) nanofiltration: will go pure liquid to filter through 270 type nanofiltration membrane, and get concentrated solution, concentrated solution concentration reaches 65%-70%, and concentrated solution is carried out the steam Sterile Filtration;
(h) spraying drying: with the concentrated solution spraying drying, must contain the glucoside amount and reach 90%, than the Steviosides product of absorbancy≤0.015.
The invention has the beneficial effects as follows: nanofiltration is between ultrafiltration and reverse osmosis, can make relative molecular weight in the solution obtain separating less than 600 material at normal temperatures, is a kind of physics removal of impurities process.It can remove small organic molecule and some microorganisms in filtration procedure, and gives inorganic salts simultaneously, integrates concentrated, dialysis and degerming.Not only can improve the purity of product greatly, and have easy and simple to handlely, working pressure is low, save energy, advantage such as pollution-free.
Description of drawings
The present invention is further described below in conjunction with embodiment.
Embodiment:
(a) pre-treatment: Folium Chrysanthemi is mixed with 50 ℃ of-55 ℃ of soft water, and soak and make the Steviosides extracting solution, through flocculation sediment and filter press two procedures, the filtrate behind the Plate Filtration is advanced next step operation;
(b) desalination: with the desalination of above-mentioned filtrate spent ion exchange resin, get the desalination filtrate of transmittance 〉=65%, specific conductivity≤6700us/cm, desalination filtrate is advanced next step operation;
(c) absorption: adopt Steviosides effective constituent in the above-mentioned desalination filtrate of resin absorption, treated back up to standard discharging of absorption waste liquid or reuse;
(d) resolve: the resin that has adsorbed Steviosides effective constituent with sodium hydroxide, salt acid treatment, with aqueous ethanolic solution the Steviosides effective constituent that is adsorbed on the resin is resolved again, must be than the desorbed solution of absorbancy≤0.65, desorbed solution advances next step operation;
(e) decolouring: gac inserted in the desorbed solution to mix stir, filter than the destainer of absorbancy≤0.16;
(f) ion-exchange: destainer is successively by after Zeo-karb and the anionite-exchange resin removal of impurities, and evaporation goes purely must remove pure liquid, goes pure liquid to advance next step operation;
(g) nanofiltration: will go pure liquid to filter through 270 type nanofiltration membrane, and get concentrated solution, concentrated solution concentration reaches 65%-70%, and concentrated solution is carried out the steam Sterile Filtration;
(h) spraying drying: with the concentrated solution spraying drying, must contain the glucoside amount and reach 90%, than the Steviosides product of absorbancy≤0.015.
The present invention and traditional concentration technology and industry average level are compared as follows:
| Technical indicator (do not indicate especially be mean value) | The tradition concentration technology | Behind the applying nano filtering technique | The industry average level |
| Than absorbance Elcml% | 0.08 | 0.015 | 0.08 |
| Sugar concentration % | 52 | 65 | 52 |
| Contain glucoside amount % | 87 | 90 | 87 |
| Impurity ratio % | 7.8 | 4 | 7.8 |
Claims (7)
1. method of utilizing the nanofiltration purifying concentrated stevioside is characterized in that the concrete operations of described method are as follows:
(a) pre-treatment: Folium Chrysanthemi is mixed with water, and soak and make the Steviosides extracting solution, through flocculation sediment and filter press two procedures, the filtrate behind the Plate Filtration is advanced next step operation;
(b) desalination: with the desalination of above-mentioned filtrate spent ion exchange resin, desalination filtrate is advanced next step operation;
(c) absorption: adopt Steviosides effective constituent in the above-mentioned desalination filtrate of resin absorption, treated back up to standard discharging of absorption waste liquid or reuse;
(d) resolve: adsorbed the resin of Steviosides effective constituent with sodium hydroxide, salt acid treatment, with aqueous ethanolic solution the Steviosides effective constituent that is adsorbed on the resin has been resolved, desorbed solution advances next step operation;
(e) decolouring: gac inserted in the desorbed solution to mix stirs, filter destainer;
(f) ion-exchange: destainer is successively by after Zeo-karb and the anionite-exchange resin removal of impurities, and evaporation goes purely must remove pure liquid, goes pure liquid to advance next step operation;
(g) nanofiltration: will go pure liquid to filter, and get concentrated solution, concentrated solution is carried out the steam Sterile Filtration through nanofiltration membrane;
(h) spraying drying:, get the Steviosides product with the nanofiltration liquid spraying drying after the Sterile Filtration.
2. the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1 is characterized in that: the water that soaks Folium Chrysanthemi is 50 ℃ of-55 ℃ of soft water.
3, the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1 is characterized in that: the transmittance of desalination filtrate 〉=65%, specific conductivity≤6700us/cm.
4, the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1 is characterized in that: ratio absorbancy≤0.65 of desorbed solution.
5, the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1 is characterized in that: ratio absorbancy≤0.16 of destainer.
6, the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1 is characterized in that: nanofiltration membrane is 270 type nanofiltration membrane.
7, the method for utilizing the nanofiltration purifying concentrated stevioside according to claim 1, it is characterized in that: concentrated solution concentration reaches 65%-70%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200910115114XA CN101508708A (en) | 2009-03-26 | 2009-03-26 | Method for purifying concentrated stevioside by using nanofiltration |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200910115114XA CN101508708A (en) | 2009-03-26 | 2009-03-26 | Method for purifying concentrated stevioside by using nanofiltration |
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| CN101508708A true CN101508708A (en) | 2009-08-19 |
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| CNA200910115114XA Pending CN101508708A (en) | 2009-03-26 | 2009-03-26 | Method for purifying concentrated stevioside by using nanofiltration |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946887A (en) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | Preparation method of stevioside |
| CN102786567A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Method for preparing high-purity stevioside through multi-column serial resins absorption and separated column analysis |
| CN102786565A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Process for purifying stevia glycosides by multi-column resin series connection and alcoholic absorption |
| CN102786566A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Method for absorbing and purifying stevioside through multiple-column secondary resin series connection |
| CN107988437A (en) * | 2017-11-29 | 2018-05-04 | 甘肃西域阳光食品有限公司 | A kind of technique that STEVIA REBAUDIANA immersion ability is strengthened by vibrating screen |
| CN108522291A (en) * | 2015-01-23 | 2018-09-14 | 谱赛科美国公司 | STEVIA REBAUDIANA new varieties 814011 compose the preparation of star 3 and high RM contents steviol glycoside |
-
2009
- 2009-03-26 CN CNA200910115114XA patent/CN101508708A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946887A (en) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | Preparation method of stevioside |
| CN101946887B (en) * | 2010-09-01 | 2012-10-03 | 桂林莱茵生物科技股份有限公司 | Preparation method of stevioside |
| CN102786567A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Method for preparing high-purity stevioside through multi-column serial resins absorption and separated column analysis |
| CN102786565A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Process for purifying stevia glycosides by multi-column resin series connection and alcoholic absorption |
| CN102786566A (en) * | 2012-09-07 | 2012-11-21 | 谱赛科（江西）生物技术有限公司 | Method for absorbing and purifying stevioside through multiple-column secondary resin series connection |
| CN108522291A (en) * | 2015-01-23 | 2018-09-14 | 谱赛科美国公司 | STEVIA REBAUDIANA new varieties 814011 compose the preparation of star 3 and high RM contents steviol glycoside |
| CN108522291B (en) * | 2015-01-23 | 2022-01-04 | 谱赛科美国公司 | Method for preparing stevioside with high RM content by using new stevia variety 814011 Puxing No. 3 |
| CN107988437A (en) * | 2017-11-29 | 2018-05-04 | 甘肃西域阳光食品有限公司 | A kind of technique that STEVIA REBAUDIANA immersion ability is strengthened by vibrating screen |
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