CN101496561A - Method for improving flavor of astaxanthin-containing extract - Google Patents
Method for improving flavor of astaxanthin-containing extract Download PDFInfo
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- CN101496561A CN101496561A CNA200910003765XA CN200910003765A CN101496561A CN 101496561 A CN101496561 A CN 101496561A CN A200910003765X A CNA200910003765X A CN A200910003765XA CN 200910003765 A CN200910003765 A CN 200910003765A CN 101496561 A CN101496561 A CN 101496561A
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Abstract
A method for improving the flavor of an astaxanthin-containing extract of the present invention includes the steps of: mixing 0.5 to 1000 parts by weight of ethanol and 1 part by weight of the astaxanthin-containing extract so as to obtain an ethanol mixture; collecting a solid precipitated from the ethanol mixture obtained; and drying the solid collected. In particular, the astaxanthin-containing extract, i.e., the starting material, is an extract from a green alga.
Description
Technical field
The present invention relates to improve the method and the astaxanthin-containing extract of smell that utilized improvement that this method obtains of the smell of astaxanthin-containing extract.
Background technology
Known astaxanthin is a kind of of red carotenoid, has strong antioxidation.Therefore, be used as food coloring, cosmetics, health food, pharmaceuticals etc.Except the astaxanthin of chemical synthesis, the astaxanthin that comes from natural products is arranged also.Astaxanthin from natural products extracts from shrimps such as krill, red shrimp, phaffiafhodozyma, algae etc.But, because the content astaxanthin in shrimps such as krill or the yeast is low, therefore, can not be from wherein extracting astaxanthin efficiently.In addition, algae can change with external environment, and sporangiocystization takes place, and accumulates astaxanthin in frond.Therefore, utilizing algae to produce the research of astaxanthin.
Owing to contain 2 hydroxyls in the astaxanthin molecule, so its monoesters that often forms to combine aliphatic acid on the hydroxyl or the form of diester exist.Especially the monoesters body is in the majority, and the episome that does not combine with aliphatic acid is a denier.This ester body is compared with episome, and the stability to light and oxygen etc. is high usually, operation easily on making, and in addition, according to circumstances, absorption in animal body is also good.
But the various chemical behaviors of the fatty acid ester of lutein such as astaxanthin are with similar as the triglycerides and the diglyceride of the neutral lipid with the ester bond that forms with aliphatic acid.And, produce in the algae of lutein, also produce a large amount of above-mentioned neutral lipids simultaneously.Therefore, if with common organic solvent extracting lutein, then have than the more neutral lipid of lutein content and extracted simultaneously.That is what, be recovered to is the neutral lipid that contains low concentration (10 quality % are following) lutein.Therefore, in order to obtain the astaxanthin of high concentration, the similar each other neutral lipid of chemical behavior need be separated with the fatty acid ester of astaxanthin.In addition, contain the neutral lipid of low concentration (10 quality % are following) lutein, with the foul smell that comes from raw material, removing this foul smell also is being important aspect the expansion purposes.
As the method that from a large amount of greases (neutral lipid), reclaims lutein, the method that has the crystallization of utilizing to reclaim.For example, patent documentation 1 discloses the astaxanthin method that crystallization is made with extra care from the chloroformic solution that contains astaxanthin that makes.In addition, patent documentation 2 discloses the lutein that makes the hydrolysis of lutein diester in the oil composition that extracts and change episome into from the petal of marigold, the method for utilizing crystallisation to make with extra care then.But, use the problems such as toxicity of the crystallisation of chloroform owing to chloroform, be unsuitable in the manufacturing process of food, using.In addition, crystallisation also exist the material demand of wanting crystallization to reach the above concentration of normal concentration, the material beyond the desirable material may crystallization, the problems such as purity reduction of crystal when target substance is difficult to form crystal structure.In addition, more than any one method, all be the method that makes the crystallization of lutein episome, thereby can not obtain the mixture of fatty acid ester of lutein.
The method (with reference to patent documentation 3～5) that will directly reclaim as precipitation with the form of ester admixture as a kind of fatty acid ester of lutein of carotenoid is disclosed.In the patent documentation 3, make the fatty acid ester of lutein that derives from plant be dissolved in the warm acetone and after removing sediment, acetone is removed in distillation, and the residual fraction that obtains is dissolved in warm propyl alcohol and/or warm butanols, makes its precipitation after the cooling and makes with extra care.In the patent documentation 4, Flos Tagetis Erectae extract is dissolved in warm ethanol, after removing by filter insoluble matter,, obtains fatty acid ester of lutein as precipitation with the filtrate cooling.In the patent documentation 5, will utilize the fatty acid ester of lutein of hydrocarbon solvent extraction to mix, make the impurity dissolving and after removing, obtain the trans-lutein fatty acid ester with ethanol.So, the various methods that are used for refining fatty acid ester of lutein are arranged, but it is still indeterminate whether fully to remove the foul smell that comes from raw material.
Studying the method for from carotenoid pigment, removing the foul smell that comes from raw material.For example, in the patent documentation 6, the material that will contain carotenoid pigment obtains solid matter with water-miscible organic solvent, use fat-soluble organic solvent mixed processing again.But, consider in food etc. and use, preferably do not use fat-soluble organic solvent.In addition, in the patent documentation 7～9, will contain the material hydrolysis of carotenoid pigment after, add carotenoid slightly solubility organic solvent (for example, ethanol, supercritical carbon dioxide) and wash.These are the methods that reclaim episome carotenoid, rather than reclaim the method for the carotenoid that exists with the ester admixture state.
Patent documentation 1: Japanese kokai publication hei 7-118226 communique
Patent documentation 2: the flat 11-508603 communique of Japanese Unexamined Patent Application Publication
Patent documentation 3: TOHKEMY 2002-30068 communique
Patent documentation 4: No. 4048203 specification of United States Patent (USP)
Patent documentation 5: the international brochure that discloses No. 99/54408
Patent documentation 6: Japanese kokai publication hei 9-48927 communique
Patent documentation 7: Japanese kokai publication hei 7-304977 communique
Patent documentation 8: Japanese kokai publication hei 7-304978 communique
Patent documentation 9: Japanese kokai publication hei 7-304979 communique
Summary of the invention
The object of the present invention is to provide the method for the smell that improves the astaxanthin-containing extract, and the astaxanthin-containing that has improved smell extract is provided.
The invention provides the method for the smell that improves the astaxanthin-containing extract, this method comprises: with respect to 1 mass parts astaxanthin-containing extract, the operation that adds 0.5 mass parts～1000 mass parts ethanol and mix; From this alcohol mixeding liquid, reclaim the operation of solid constituent; And the operation of the solid constituent of this recovery being carried out drying.
In one embodiment, above-mentioned astaxanthin-containing extract is a green algal extract.
More specifically, the above-mentioned green alga in the above-mentioned embodiment is the unicellular alga that belongs to haematococcus.
In one embodiment, the ethanol that adds in the above-mentioned mixed processes is preferably 1 mass parts～100 mass parts, more preferably 2 mass parts～50 mass parts, more preferably 4 mass parts～30 mass parts.
In one embodiment, the ethanol that adds in the above-mentioned mixed processes has the above purity of 99% (v/v).
In one embodiment, the temperature of the above-mentioned alcohol mixeding liquid in the above-mentioned recovery process is below 10 ℃.
In one embodiment, above-mentioned mixed processes is under the temperature below 60 ℃, more preferably carry out under the temperature between 5 ℃～50 ℃.
The present invention also provides the astaxanthin-containing extract of smell that utilized improvement that above-mentioned arbitrary method obtains.
The invention effect
According to the present invention, can be by the improved astaxanthin-containing extract of the distinctive odor that comes from the raw material that contains astaxanthin of very simple operation.Therefore, the astaxanthin-containing extract that has improved smell of the present invention is preferably used as the material to conditional food of smell or pharmaceuticals.
Description of drawings
Fig. 1 is the curve map of the relation between the astaxanthin concentration in expression amount of alcohol and the dry thing that obtains.
Fig. 2 is the gas chromatogram of the smell of the haematococcus extract (product are untreated) that obtains in the reference example 1.
Fig. 3 is the gas chromatogram that the smell after 98% (v/v) Ethanol Treatment that obtains among the embodiment 6 improves the smell of product.
Fig. 4 is the gas chromatogram that the smell after 99% (v/v) Ethanol Treatment that obtains among the embodiment 6 improves the smell of product.
The specific embodiment
(astaxanthin-containing extract)
Among the present invention, only be called under the situation of astaxanthin, be meant at least a compound in the group that the aliphatic acid diester of the fatty-acid monoester of the episome that is selected from astaxanthin, astaxanthin and astaxanthin forms.As the aliphatic acid that can constitute the astaxanthin fatty acid ester, can enumerate laurate, myristic acid, pentadecanoic acid, palmitic acid, palmitoleic acid, Heptadecanoic acide, elaidic acid, castor oil acid, petroselic acid, vaccenic acid, eleostearic acid, punicic acid, licanic acid, parinaric acid, gadoleic acid, the 5-eicosenoic acid, the 5-docosenoic acid, cetoleic acid, erucic acid, 5,13-two dodecadienoic acids, shark acid, decylenic acid, dodecenoic acid, oleic acid, stearic acid, eicosapentaenoic acid, DHA, linoleic acid, alpha-linolenic acid, arachidonic acid etc.
Among the present invention, the astaxanthin-containing extract is meant the oily matter that contains astaxanthin that extracts from natural products.Can be the oily matter that has only carried out extraction procedure (that is, except that extraction procedure, no longer carrying out concentrating, making with extra care of astaxanthin), perhaps also can be to have carried out the oily matter that concentrates, makes with extra care.As natural products, can enumerate the red yeast that contains astaxanthin; Crustacean shells such as the Japan violent water flea of tiger spot (red water flea), krill; Microalgaes such as green algae etc.Among the present invention, the preferred extract that derives from green alga that uses.
For green alga, get final product so long as have the green alga that produces the astaxanthin ability, have no particular limits.For example, the unicellular alga of preferably using haematococcus (Haematococcus) to belong to.As preferred green alga, can enumerate haematococcus pluvialis (H.pluvialis), lake living haematococcus (H.lacustris), Haematococcus capensis (H.capensis), Haematococcusdroebakensi (H.droebakensi) and Haematococcus zimbabwiensis (H.zimbabwiensis) etc.
For example, the astaxanthin-containing extract that from green alga, prepares as described below.With the green alga of sporangiocystization, in nutrient medium, under carbon dioxide supplies with, with for example 8000 μ mol-photon/m
3/ second above effective light quantum the bundle of light compositing is given with amount and is carried out light-struckly cultivating simultaneously, extracts the oily matter that contains astaxanthin then.Extracting method for the oily matter that contains astaxanthin has no particular limits, the method that can use those skilled in the art to use always.For example, from the green alga of cultivating, utilize solvent extraction, Mechanical Crushing (for example, pearl is ground formula tissue grinder etc.) or squeezing and extracting is carried out in above method combination.As solvent, can use organic solvents such as chloroform, hexane, acetone, methyl alcohol, ethanol.Perhaps, also can adopt the supercritical extraction method to come extracting.Utilize under the situation of solvent extraction, after the extracting, can remove solvent by the method that those skilled in the art use always.
In the algae such as haematococcus, in frond, accumulate, but the while also produces oily matters such as neutral lipid as a kind of astaxanthin of lutein.Therefore, extract in the extraction process of astaxanthin being generally used for, astaxanthin and neutral grease etc. is together extracted.Come from astaxanthin concentration in the oily matter of algae according to difference to some extent such as extraction solvent, method for extracting, be generally about 1 quality %～about 10 quality %.In addition, the astaxanthin of algae is according to the difference of condition of culture and difference, but is principal component with the astaxanthin fatty-acid monoester, accounts for more than at least 50 quality % of all lutein classes, more preferably more than the 60 quality %, more preferably more than the 70 quality %.The content of astaxanthin aliphatic acid diester is about 15 quality %～about 30 quality %, and the astaxanthin of episome (dissociating) is many below 1 quality %.Among the present invention,, preferably use the oily matter that contains astaxanthin in the algae that belongs to haematococcus in the green algae, preferred haematococcus pluvialis source as the astaxanthin-containing extract.
(the smell improvement method of astaxanthin-containing extract)
The smell improvement method of astaxanthin-containing extract of the present invention comprises: with respect to 1 mass parts astaxanthin-containing extract, the operation that adds 0.5 mass parts～1000 mass parts ethanol and mix; From this alcohol mixeding liquid, reclaim the operation of solid constituent; And the operation of the solid constituent of this recovery being carried out drying.
Among the present invention, utilize astaxanthin to be insoluble in the character of ethanol, remove the odour component of the uniqueness that dissolves in ethanol, and reclaim the material that is insoluble to ethanol.
In the smell improvement method of the present invention, at first, the astaxanthin-containing extract is mixed with ethanol.The astaxanthin-containing extract that uses is the aforesaid oily matter that contains astaxanthin that extracts from natural products.
The amount of alcohol of using in the mixing is big more, and the purity of astaxanthin is high more, and distinctive smell is more little in the astaxanthin-containing extract, smell to improve effect good more.And on the other hand, consumption of ethanol is many more, and the organic efficiency of astaxanthin is low more.Therefore, consumption of ethanol is 0.5 mass parts～1000 mass parts with respect to 1 mass parts astaxanthin-containing extract, is preferably 1 mass parts～100 mass parts, more preferably 2 mass parts～50 mass parts, 4 mass parts～30 mass parts more preferably, also 6 mass parts～20 mass parts more preferably.
In addition, the purity of the ethanol that uses in the mixing is high more, and the astaxanthin purity of recovery is then high more, and distinctive smell is more little in the astaxanthin-containing extract, smell to improve effect good more.Therefore, the ethanol that uses among the present invention has and is preferably above, above, the above purity of 99.5% (v/v) more preferably of 99% (v/v) more preferably of 98% (v/v).
There is no particular limitation for mixed method.Can for example adopt stir, method such as vibration mixes.The purpose of this mixed processes is, makes the substance dissolves that is soluble in ethanol in the astaxanthin-containing extract in ethanol, therefore, need not to carry out violent especially mixing, stirring, and for example, gentle stirring gets final product.Can adopt the conventional mixing of using of those skilled in the art, for example, utilize agitator to stir, utilize magnetic stirrer, utilize ultrasonic irradiation to mix, utilize oscillator vibration etc.In mixed processes, in order to reduce the viscosity of astaxanthin-containing extract, mix with ethanol being easy to, mix preferably under the temperature below 60 ℃, more preferably under the temperature between 5 ℃～50 ℃, carry out.When temperature is too high, may make the generation deteriorations such as grease in the astaxanthin-containing extract, the smell variation, the concentration of the astaxanthin in the extract that obtains and the rate of recovery reduce.If temperature is low excessively, then the astaxanthin-containing extract is difficult to mix with ethanol.
Then, from alcohol mixeding liquid, reclaim solid constituent.The temperature of the alcohol mixeding liquid during recovery is preferably normal temperature or the following temperature of normal temperature, more preferably below 10 ℃, more preferably below 5 ℃.In order to be easy to reclaim solid constituent, also can leave standstill.Time of repose does not have specific restriction.For example, when leaving standstill under 5 ℃, solid constituent precipitated in about 1 hour～about 48 hours.
The frozen composition that produces adopts methods such as for example filtration, centrifugation, decant to reclaim.Then, the solid constituent that reclaims under reduced pressure or by feeding inert gas such as nitrogen etc. is removed ethanol, and make its drying.
(having improved the astaxanthin-containing extract of smell)
The improvement that obtains as mentioned above in the astaxanthin-containing extract of smell, content astaxanthin is according to difference as the difference of the astaxanthin concentration in the astaxanthin-containing extract of initial substance and to some extent, remember with episome, be generally at least 10 quality %, be preferably at least 15 quality %, more preferably more than at least 20 quality %, more preferably more than at least 25 quality %.The astaxanthin that this has improved in the extract of smell depends on the astaxanthin in the raw material of astaxanthin-containing extract, is astaxanthin episome, astaxanthin fatty-acid monoester, astaxanthin aliphatic acid diester and their mixture.For example, be under the situation of raw material at haematococcus, the over half of astaxanthin can be fatty-acid monoester.
In addition, improved in the astaxanthin-containing extract of smell, the smell that comes from the uniqueness of raw material can be improved to only residual a little or almost or the degree that does not have fully.
[embodiment]
Below, enumerate and use the embodiment of haematococcus pluvialis K0084 strain that the present invention is described, but the invention is not restricted to this embodiment.In addition, in the present embodiment, the concentration of astaxanthin and the sensory evaluation of smell adopt following method to measure.
(assay method of astaxanthin concentration)
Astaxanthin concentration in the extract (the astaxanthin fatty acid ester being scaled the concentration of free astaxanthin) after suitably diluting with DMSO, is tried to achieve by following computing formula.
Astaxanthin concentration (quality %)=(A*100*F)/(W*2085)
At this, A is the absorbance (optical path length 1cm) of sample at the 478nm place, and F is the extension rate of sample, and W is the quality (g) of sample.
(sensory evaluation method)
To the sample that obtains, adopt the distinctive smell of following benchmark evaluation haematococcus.In addition, this evaluation is by taste test (five tastes discrimination test) and olfactory test (T﹠amp; T olfactometer method) all qualified estimator that can carry out normal sensory evaluation carries out.
The standard that smell is estimated
1 ... there is not distinctive smell fully
2 ... almost there is not distinctive smell
3 ... a little distinctive smell is arranged slightly
4 ... strong distinctive smell is arranged
5 ... very strong distinctive smell is arranged
(reference example 1: the preparation of haematococcus extract)
Haematococcus pluvialis K0084 strain is inoculated in the culture medium (low nutrient medium) with composition shown in the following table 1.
[table 1]
| Composition | g/L |
| KNO 3 | 0.41 |
| K 2HPO 4 | 0.04 |
| MgSO 4·7H 2O | 0.075 |
| CaCl 2·2H 2O | 0.036 |
| Citric acid (anhydrous) | 0.006 |
| Ferrum citricum (III) ammonium | 0.006 |
| EDTA·2Na | 0.001 |
| Na 2CO 3 | 0.02 |
| CuSO 4·5H 2O | 0.00286 |
| H 3BO 4 | 0.00181 |
| MnCl 2·4H 2O | 0.00022 |
| ZnSO 4·7H 2O | 0.00008 |
| Na 2MoO 4 | 0.000021 |
| Co(NO 3) 2·6H 2O | 0.000000494 |
Particularly, in the flat blake bottle of 1.5L, add the low nutrient medium of 1L, and the K0084 strain of inoculation sporangiocystization.The following cultivation: use white fluorescent lamp, with 25000 μ mol-p/m
3The effective light quantum bundle of light compositing of/second is carried out illumination with amount, and contains 3 volume %CO with 0.5L/ minute speed feeding
2Air (that is, ventilation speed is 0.5vvm), cultivated 7 days down at 25 ℃.After 7 days, collect the K0084 strain of sporangiocystization, it is adjusted to 1.5 * 10 with low nutrient medium
6The concentration of individual/ml.
With with the inwall of culture tank at interval the mode of 3cm be oppositely arranged in the flat culture tank of acrylic acid transparent panel, add the low nutrient medium of 9L, and with 1.5 * 10
5The above-mentioned sporangiocyst K0084 of the concentration inoculation 1L strain of individual/ml begins to cultivate.Cultivate and carry out under the following conditions: 6 pipe white fluorescent lamps respectively are set, in the both sides of flat culture tank with 25000 μ mol-p/m
3The effective light quantum bundle of light compositing of/second is shone with amount, and contains 3 volume %CO with 0.5L/ minute speed feeding
2Air (that is, ventilation speed is 0.5vvm), cultivated 21 days down at 25 ℃.Take out nutrient solution, wash frond with water, be collected in the pipe of pearl mill formula tissue grinder special use.After in this pipe, adding the zirconium dioxide pearl, add 50mL ethanol again, carry out fragmentation with pearl mill formula tissue grinder.Broken thing is separated into cleer and peaceful sediment with centrifugal separator, and reclaims supernatant.In sediment, add 50mL ethanol once more, repeat and above-mentioned same operation, almost completely bleach until sedimentary color.The each several part ethanol that reclaims is merged, and ethanol is removed in decompression, obtains green algal extract.Astaxanthin concentration in this extract with episome content note, is 13.1 quality %.
(embodiment 1: improved the preparation-1 of the astaxanthin-containing extract of smell)
The haematococcus extract (astaxanthin episome content is 13.1 quality %) that obtains in the above-mentioned reference example 1 of 30g is heated to 50 ℃,, uses magnetic stirring apparatus fully to stir, mix to wherein adding 240g ethanol (purity is more than 99.5% (v/v)).This solution after 5 ℃ of following standing over night, is reclaimed the solid constituent of separating out with the filter paper filtration.Decompression concentrates the solid constituent that obtains, and removes ethanol.Obtaining astaxanthin episome content is the dry thing 6.8g (rate of recovery: 54%) of 31.5 quality %.
(embodiment 2: improved the preparation-2 of the astaxanthin-containing extract of smell)
The dwell temperature of the ethanolic solution of the haematococcus extract among the embodiment 1 is made as normal temperature, in addition, all handles similarly to Example 1, obtaining astaxanthin episome content is the dry thing 5.0g (rate of recovery: 44%) of 34.8 quality %.
(embodiment 3: improved the smell evaluation of the astaxanthin-containing extract of smell)
To the improvement that obtains in the haematococcus extract that obtains in the above-mentioned reference example 1 and embodiment 1 and 2 each sample of astaxanthin-containing extract of smell, carry out the sensory evaluation of haematococcus distinctive smell.The results are shown in table 2.
[table 2]
| Sample | The evaluation of smell |
| The haematococcus extract | 5 |
| The dry thing of embodiment 1 | 2 |
| The dry thing of embodiment 2 | 3 |
The haematococcus extract has very strong distinctive smell, after Ethanol Treatment, can be improved to only residual a little smell or almost not have the degree of smell.
(embodiment 4: the research of the amount of alcohol of use)
At normal temperatures, add the ethanol (purity is more than 99.5% (v/v)) of the various amounts of 2～50 mass parts in haematococcus extract 1 mass parts that in above-mentioned reference example 1, obtains, use agitator fully to stir, mix.This solution after 5～10 ℃ of following standing over night, is reclaimed the solid constituent of separating out with the filter paper filtration.Decompression concentrates the solid constituent that reclaims, and obtains dry thing to remove ethanol.To the dry thing that obtains, measure its astaxanthin concentration, and carry out the sensory evaluation of haematococcus distinctive smell.The relation of the astaxanthin concentration in amount of alcohol of using and the dry thing that obtains is shown in Fig. 1.In addition, the result who has also shown the dry thing that obtains in the foregoing description 1 and 2 among Fig. 1 simultaneously.In addition, the sensory evaluation result of the smell of the dry thing that obtains in the present embodiment 4 together is shown in table 3 with the astaxanthin concentration and the rate of recovery.
[table 3]
Following as can be seen tendency: the amount of alcohol of use is many more, and the astaxanthin concentration in the dry thing is high more, and simultaneously, smell improves also obvious more.On the other hand, the rate of recovery of astaxanthin has the tendency of reduction as can be known.
(embodiment 5: the research of the temperature of mixed processes)
At normal temperatures, in haematococcus extract 1 mass parts that in above-mentioned reference example 1, obtains, add the ethanol (purity is more than 99.5% (v/v)) of 10 mass parts, and under different temperatures (10～60 ℃), fully stir, mix with agitator.This solution after 10 ℃ of following standing over night, is reclaimed the solid constituent of separating out with the filter paper filtration.Decompression concentrates the solid constituent that reclaims, and obtains dry thing to remove ethanol.To the dry thing that obtains, measure its astaxanthin concentration, and carry out the sensory evaluation of haematococcus distinctive smell.The results are shown in table 4.In addition, mix down and during standing over night,, therefore left standstill for two evenings because it is few to estimate solid constituent at 60 ℃.
[table 4]
As can be seen, if mixing temperature below 60 ℃, the smell of the dry thing that obtains and astaxanthin concentration do not have significant difference.
(embodiment 6: the research of purity of alcohol)
At normal temperatures, add the ethanol of the various purity of 10 mass parts in haematococcus extract 1 mass parts that in above-mentioned reference example 1, obtains, fully stir, mix with agitator.This solution after 10 ℃ of following standing over night, is reclaimed the solid constituent of separating out with the filter paper filtration.Decompression concentrates the solid constituent that reclaims, and obtains dry thing to remove the second alcohol and water.To the dry thing that obtains, measure its astaxanthin concentration, and carry out the sensory evaluation of haematococcus distinctive smell.The results are shown in table 5.
[table 5]
As can be seen, the purity of the ethanol of use is high more, and the astaxanthin concentration in the dry thing is then high more, and simultaneously, smell improves also obvious more.
(embodiment 7: the research of the solvent beyond the ethanol)
At normal temperatures, add the ethanol (purity is more than 99.5% (v/v)) or the 2-propyl alcohol of 10 mass parts in haematococcus extract 1 mass parts that in above-mentioned reference example 1, obtains, fully stir, mix with agitator.This solution after leaving standstill 3 hours under 5 ℃, is fully stirred once more.(quantitative filter paper φ 90mmNo.5C: Bu Shi (Buchner) funnel ア De バ Application テ Star Network Co., Ltd.) once reclaims the solid constituent of separating out by filtration under diminished pressure to allow this flow of solution cross to be equipped with filter paper.At this moment, instrumentation filters the time that needs.Then, decompression concentrates the solid constituent that reclaims, and obtains dry thing to remove ethanol or 2-propyl alcohol.To the dry thing that obtains, measure its astaxanthin concentration, and carry out the sensory evaluation of haematococcus distinctive smell.The results are shown in table 6.
[table 6]
Under the situation of using the 2-propyl alcohol, also can access the dry thing that astaxanthin concentration height and smell have improved.But filter paper stops up, and the time that filtration needs is very long.Therefore as can be known, the solvent beyond the ethanol is unsuitable for actual industrial use.
(embodiment 8: odorous substance 2, the mensuration of 4-heptadienal)
Contain the smell of the uniqueness that the raw material of astaxanthin sends, derive from multiple odorous substance, as one of its reason material, can enumerate 2, the 4-heptadienal.2, the 4-heptadienal is considered to one of reason material of the stench flavor of running water (reading it with reference to the sheet hilllock, the 47th national running water research presentations summary collection,, 472-473 page or leaf in 1996).Therefore, the dry thing after 98% (v/v) and 99% (v/v) Ethanol Treatment that obtains in the haematococcus extract (product are untreated) that above-mentioned reference example 1 is obtained and the foregoing description 6 (smell improves product) carries out 2, the mensuration of 4-heptadienal.
Each sample of 0.5g is incubated under 60 ℃ the time to wherein feeding nitrogen, and reclaims gas.Make this gas of 1L by adsorbent (NeedlEx: letter and processing Co., Ltd.), odour component is adsorbed on the adsorbent.Employing possesses the gas chromatograph (GC) of hydrogen flame ionization detector (FID) odour component of this absorption is analyzed.Analysis condition is as follows.
(GC)
Post: ZB-1 (60m * 0.32mmI.D.df=1.0 μ m)
Column temperature: 40 ℃ (2 minutes) → (10 ℃/minute) → 300 ℃ (5 minutes)
Inlet temperature: 250 ℃
Carrier gas (He) pressure: 121.4kPa
(FID)
Detector temperature: 300 ℃
Make-up gas (He) flow: 30.0ml/ minute
The gas chromatogram of each sample is shown in Fig. 2～4.By these figure as can be known, smell improves product to be compared with the product of being untreated, and 2, the 4-heptadienal reduces significantly.This result is very consistent with the result of sensory evaluation.
Utilize possibility on the industry
The method according to this invention can be improved the distinctive smell that derives from raw material that the astaxanthin-containing extract has. Therefore, resulting improvement the astaxanthin-containing extract of smell can be applicable to the fields such as food, pharmaceuticals, cosmetics.
Claims (17)
1. method of improving the smell of astaxanthin-containing extract, it comprises:
With respect to 1 mass parts astaxanthin-containing extract, the operation that adds 0.5 mass parts～1000 mass parts ethanol and mix; From this alcohol mixeding liquid, reclaim the operation of solid constituent; And the operation of the solid constituent of this recovery being carried out drying.
2. the method for claim 1, wherein described astaxanthin-containing extract is a green algal extract.
3. method as claimed in claim 2, wherein, described green alga is the unicellular alga that belongs to haematococcus.
4. the ethanol that adds in the method for claim 1, wherein described mixed processes is 1 mass parts～100 mass parts.
5. method as claimed in claim 4, wherein, the ethanol that adds in the described mixed processes is 2 mass parts～50 mass parts.
6. method as claimed in claim 5, wherein, the ethanol that adds in the described mixed processes is 4 mass parts～30 mass parts.
7. the ethanol that adds in the method for claim 1, wherein described mixed processes has the above purity of 99% (v/v).
8. method as claimed in claim 6, wherein, the ethanol that adds in the described mixed processes has the above purity of 99% (v/v).
9. in the method for claim 1, wherein described recovery process, the temperature of described alcohol mixeding liquid is below 10 ℃.
10. the method for claim 1, wherein described mixed processes carries out under the temperature below 60 ℃.
11. method as claimed in claim 10, wherein, described mixed processes carries out under the temperature between 5 ℃～50 ℃.
12. method as claimed in claim 11, wherein, the ethanol that adds in the described mixed processes is 4 mass parts～30 mass parts.
13. method as claimed in claim 11, wherein, the ethanol that adds in the described mixed processes has the above purity of 99% (v/v).
14. an astaxanthin-containing extract that has improved smell, it obtains by the described method of claim 1.
15. the astaxanthin-containing extract that has improved smell as claimed in claim 14, wherein, the ethanol that adds in the described mixed processes is 4 mass parts～30 mass parts.
16. the astaxanthin-containing extract that has improved smell as claimed in claim 14, wherein, the ethanol that adds in the described mixed processes has the above purity of 99% (v/v).
17. the astaxanthin-containing extract that has improved smell as claimed in claim 14, wherein, described mixed processes carries out under the temperature between 5 ℃～50 ℃.
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