CN101482552A - In-situ measurement apparatus for macroscopic algae photosynthesis rate - Google Patents
In-situ measurement apparatus for macroscopic algae photosynthesis rate Download PDFInfo
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- CN101482552A CN101482552A CNA2008100138321A CN200810013832A CN101482552A CN 101482552 A CN101482552 A CN 101482552A CN A2008100138321 A CNA2008100138321 A CN A2008100138321A CN 200810013832 A CN200810013832 A CN 200810013832A CN 101482552 A CN101482552 A CN 101482552A
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- bottle
- cover plate
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- bottle stopper
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Abstract
The invention relates to an aquiculture basis experimental technique, especially an in-situ macroscopic algae photosynthesis speed measurement device, comprising a bottle and a bottle plug, wherein the bottle is of cylinder shape and a bottle bottom is bonded on the lower end of the bottle and for closing the bottom of cylinder; an upper cover plate is bonded on the upper end of the bottle in fixed connection with the bottle and a round open is set in the center of the upper cover plate and the internal thread is on the side wall of the round open; the bottle plug is round and the outer diameter size is same with the inner diameter size of the round open of the upper cover plate and an external thread is set the outer wall of the lower end and the upper cover plate is screwed with the internal thread and the external thread; the bottle, bottle bottom and bottle plug are formed by the transparent organic glass with better transparence; a groove is set on the outer wall of the upper end of the bottle and the a steel ring is studded in the groove and a fixation steel sheet with small hole is fixed on the steel ring. The aquiculture basis experimental technique has simpleness, practicality, accurate measurement result.
Description
Technical field
The present invention relates to aquaculture infrastest technology, the photosynthetic device of specifically a kind of on-site measurement macro.
Background technology
The photosynthetic carbon fixation of algae constitutes the basis of waters primary productivity.The research of the solid carbon of algae is the pith of Ecological Physiology in Algae, and is significant to the effect of understanding algae in the material cycle of waters.Mainly be research to the research of algae photosynthesis and primary productivity to the common vetch algae in the past, and work mainly is to carry out in the laboratory, photosynthesis research to macro especially ocean macro is less, and the photosynthetic research of work place study macro still less.Atmospheric CO in recent years particularly
2Concentration raises and the relation in biosphere, waters receives much attention, and people pay attention to gradually to the Ecology Action of macro, and the photosynthetic carbon fixation of algae is to Atmospheric CO
2The influence of concentration and effect etc. are one of problems of being concerned about of present scientific circles.Because the algae photosynthesis ability is relevant with several factors, carry out work place study and can disclose its photosynthetic Changing Pattern more exactly, the physiological ecology data of macro are replenished.At present the photosynthesis rate of macro is measured and how in the laboratory, to be carried out,, obtain the oxygen-producing amount in the algae unit interval, measure its photosynthesis speed by quantitative test to dissolved oxygen DO before and after the experiment.The mensuration of dissolved oxygen DO adopts oxygen electrode method, Winkler titrimetry, the saturating membranous spectrometer of gas and inspection container etc. usually.Each is variant for the size and shape of the reactive tank of using when oxygen electrode itself and photosynthesis measurement, but ultimate principle is identical, all be to measure the variation of dissolved oxygen concentration in the experiment water body in the certain hour, obtain photosynthetic speed according to variation, experimental period and the experiment water body volume of dissolved oxygen concentration.
Summary of the invention
The object of the present invention is to provide a kind of simple in structure, photosynthetic device of on-site measurement that can relatively accurate reaction macro photosynthesis characteristics, on-site measurement can be compared the accurate response macro photosynthesis ability under the condition at the scene, and envirment factor is to photosynthetic influence.
For achieving the above object, the technical solution used in the present invention is:
A kind of device of on-site measurement macro photosynthesis rate comprises bottle, bottle stopper.
Bottle is a cylinder-like structure, and the bottle lower end is bonded with the bottle end affixed with bottle that is used for airtight cylinder bottom;
Bottle upper end is bonded with the upper cover plate with the affixed bottle of bottle, and the upper cover plate center is provided with round mouth, the sidewall of the round mouth internal thread of having got on the bus;
Bottle stopper is circular, and its external diameter is identical with the internal diameter size of upper cover plate round mouth, and the lower end outer wall is provided with external thread, is screwed by internal thread and external thread between upper cover plate and bottle stopper;
Bottle, the bottle end and bottle stopper by light transmission preferably transparent organic glass make;
Bottle upper end outer wall is provided with groove, is inlaid with steel ring in the groove, is fixed with the fixedly steel disc that has aperture on the steel ring.
Unlatching for convenient bottle stopper, above bottle stopper, be adhesive with the poly (methyl methacrylate) plate of 6 dihedrals, the poly (methyl methacrylate) plate of bottle stopper and 6 dihedrals constitutes a bottle cap, in order to reinforce the firm degree that engages between them, placed a bolt at bottle stopper and 6 dihedral poly (methyl methacrylate) plate centers, the bolt lower end is connected with the suspension ring that are used to hang the experiment biological sample.
On the upper cover plate of bottle, be the center of circle with the center of round mouth, be provided with annular groove in the periphery of round mouth, be placed with O-ring seal in it; After upper cover plate and bottle stopper screwed togather, 6 dihedral poly (methyl methacrylate) plates were pressed on the O-ring seal.
The fixedly steel disc that has aperture is 1-6, and it evenly is fixed on the steel ring by screw, has fixedly placed rope in the aperture of steel disc.
The lower sides of bottle is provided with apopore; Be connected with emulsion tube on the apopore, emulsion tube is provided with the clip that prevents that water from flowing out, and perhaps inserts one section plexi-glass tubular as rising pipe in apopore, and rising pipe is provided with the switch of a ground.
The present invention has following advantage:
1. simple and practical.Apparatus of the present invention are made simple, and transparent bottle is connected the permeability that has guaranteed light with seamless, and has certain impact resistance, is adapted at the photosynthesis ability of sea area on-site measurement macro.
2. easy to operate.Have aperture on the fixedly steel disc of apparatus of the present invention, make things convenient for rope to be connected with it, can be more firm fixing, the danger of avoiding marine high sea that it is come off; Suspension ring on the bottle cap can be fixed the experiment algae eaily, and frond is stretched, and can avoid simultaneously owing to frond is concentrated the local nutriment shortage that causes; Hexagonal bottle cap design can be outwarded winding from bottle by the aspect bottle cap.Be provided with the aperture (on connect emulsion tube) of water outlet near side under the bottle, make things convenient for water sampling.
3. good airproof performance.Apparatus of the present invention bottle is organic glass cylinder independently, and the upper cover plate and the bottle end and bottle be seamless connection of employing directly.There is a grommet type seals upper cover plate top, screw bottle stopper after, the sexangle glass plate of bottle stopper top is pressed on the O-ring seal, thereby makes whole device sealing.
4. measurement result is accurate.Apparatus of the present invention can hang on the culture raft frame by rope, and the photosynthesis rate of on-site measurement macro can react the photosynthesis ability of macro under natural environmental condition more accurately.Can pass through to adjust the photosynthesis rate of the length measurment marine alga of rope at different water layers, and suspension and cultivation site, this device is in the motion water in bottle body about by trend etc., the oxygen that marine alga photosynthesis is produced can be dissolved in the water uniformly, reduce the too high phenomenon of partial oxygen and take place, therefore can obtain result comparatively accurately.
Description of drawings
Fig. 1 photosynthesis device synoptic diagram;
The asparagus photosynthesis rate seasonal variations of Fig. 2 on-site measurement.
Embodiment
Embodiment 1
As shown in Figure 1, a kind of device of on-site measurement macro photosynthesis rate comprises bottle 1, bottle stopper 10,
Bottle 1 is a cylinder-like structure, and the bottle lower end is bonded with the bottle end 2 affixed with bottle that is used for airtight cylinder bottom;
Bottle 1 upper end is bonded with the upper cover plate 11 with the affixed bottle of bottle, and upper cover plate 11 centers are provided with round mouth, the sidewall of the round mouth internal thread of having got on the bus;
Bottle 1 upper end outer wall is provided with groove, is inlaid with steel ring 7 in the groove, is fixed with the fixedly steel disc 8 that has aperture 6 on the steel ring 7.
Unlatching for convenient bottle stopper, above bottle stopper 10, be adhesive with the poly (methyl methacrylate) plate 3 of 6 dihedrals, the poly (methyl methacrylate) plate 3 of bottle stopper 10 and 6 dihedrals constitutes a bottle cap, in order to reinforce the firm degree that engages between them, placed a bolt 9 at bottle stopper and 6 dihedral poly (methyl methacrylate) plate centers, the bolt lower end is connected with the suspension ring 4 that are used to hang the experiment biological sample;
On the upper cover plate of bottle, be the center of circle with the center of round mouth, be provided with annular groove in the periphery of round mouth, be placed with O-ring seal 5 in it; After upper cover plate and bottle stopper screwed togather, 6 dihedral poly (methyl methacrylate) plates were pressed on the O-ring seal; The fixedly steel disc 8 that has aperture is 1-6, and it evenly is fixed on the steel ring 7 by screw, has fixedly placed rope in the aperture 6 of steel disc 8.
The lower sides of bottle 1 is provided with apopore, is connected with emulsion tube on the apopore, and emulsion tube is provided with the clip that prevents that water from flowing out.
Concrete manufacturing process:
The upper cover plate of the bottle of photosynthesis device, the bottle end, bottle and bottle cap all are with light transmission transparent organic glass making preferably.
Bottle: be diameter 90mm, thickness is that the plexi-glass tubular of 3mm is made;
The bottle end: be the circular poly (methyl methacrylate) plate of diameter 90mm, thickness is 15mm, and all sidecars go to the thick edge of 3mm, 10mm, carry out seamless bonding with bottle again;
The upper cover plate of bottle: it handles identical with material and the method that adopt at the bottle end, difference is, at the center of upper cover plate car the round mouth of diameter 50mm, the sidewall of the round mouth internal thread of having got on the bus arranged, dig out wide annular groove at 70mm place, the center of distance upper cover plate, be placed with O-ring seal in it into 2mm;
Bottle cap: be one to have the organic glass bottle stopper of externally threaded 50mm (matching) with above-mentioned round mouth internal diameter, open for convenient, be adhesive with on the bottle stopper on the poly (methyl methacrylate) plate of 6 dihedrals, in order to reinforce the firm degree that engages between them, at bottle stopper with have between the poly (methyl methacrylate) plate of 6 dihedrals and placed bolt, two parts are fixed (carrying out encapsulation process by rubber cushion) by bolt, the bolt lower end connects suspension ring and is used for hanging the experiment biology.
The make progress sidewall at 20-30mm place of the bottom surface of bottle is provided with an apopore, is connected with an emulsion tube on the apopore, and emulsion tube is provided with the clip that prevents that water from flowing out.
Stationary installation: 20mm goes out above the distance bottle, it is 15mm that car goes out width, the groove of degree of depth 1.5mm, fixed steelring is fixed in the groove, the fixedly steel disc that has aperture is fixed on the steel ring by screw, during experiment rope tied up on the aperture on the steel disc, tie up to the on-site measurement of carrying out light and effect on the culture raft frame then.
Application examples:
In the 4-11 month in 2002, in Sang Gou gulf, Rongcheng City, Shandong Province, with the seasonal variations of the photosynthesis bottle on-site measurement asparagus photosynthesis rate that designs voluntarily.(30～50cm) healthy asparagus is cleaned with seawater and hangs in the photosynthesis bottle with certain weight in wet base (about 5g) and length, sealed suspension is in depth of water 1.0m～1.5m place (upper cover plate with bottle is as the criterion), control group is set, every group of 3 repetitions, place water 2～3h, measure the illumination of scene temperature, salinity and experimental session.Experiment is taken out asparagus after finishing immediately, analyzes with the siphonage water sampling, comprises dissolved oxygen DO hydration index such as (DO), and water sample is fixing immediately.Asparagus photosynthesis carbon assimilation speed is produced O by photosynthetic entropy (1.2) by photosynthesis
2Amount is calculated.Photosynthesis rate calculates by producing oxygen: O
2(mg/gdwh)=(O
2'-O
2) * V
0/ W
0/ t (O
2' be to test the photosynthesis bottle O that adds asparagus when finishing
2Concentration (mg/l), O
2Be the O of blank
2Concentration (mg/l), V
0Be the volume (1) of photosynthesis bottle, W
0Be the dry weight (g) of experiment asparagus, t is experimental period (h)).Use iodometric determination DO, asparagus sample filter paper suck dry moisture is measured weight in wet base, dries 24h down to weight in 65 ℃ in drying box.
As can be seen from Figure 2, the photosynthesis rate of asparagus has distinct seasonal variation.In April, asparagus photosynthesis rate (seeing Fig. 2 column diagram) is minimum, is 0.4mgO
2/ gdwh; In July, its photosynthesis ability is the strongest, is 14.4mgO
2/ gdwh, the solid carbon rate of photosynthesis (calculating with photosynthetic quotient 1.2) is 17.3mgCO
2/ gdw.h; Secondly be June, than May, the 8-9 month is big slightly.Nature photosynthesis and water temperature are closely related.In April, average water temperature has only 7.7 ℃, exceeds the lower limit of asparagus comfort zone, so its photosynthesis ability is minimum; 7 monthly average water temperatures are 21.2 ℃, are the preference temperatures of its growth, and its photosynthesis rate is also maximum; Higher 21.6～25.6 ℃ of coolant-temperature gage in August (average 23.7 ℃), photosynthesis rate descends to some extent, but still higher (9.23mgO
2/ gdwh).The oxygen content (seeing Fig. 2 broken line graph) of as can be seen from the figure culturing in the waters descends from the May to the August gradually, rise to some extent then, and asparagus all has higher photosynthesis product oxygen speed the 5-9 month, can just remedy because therefore the high oxygen utilization of this time aquaculture organism has great importance.
Claims (5)
1. the device of an on-site measurement macro photosynthesis rate comprises bottle (1), bottle stopper (10), it is characterized in that:
Bottle (1) is a cylinder-like structure, and the bottle lower end is bonded with the bottle end (2) affixed with bottle that is used for airtight cylinder bottom;
Bottle (1) upper end is bonded with the upper cover plate (11) with the affixed bottle of bottle, and upper cover plate (11) center is provided with round mouth, the sidewall of the round mouth internal thread of having got on the bus;
Bottle stopper (10) is circular, and its external diameter is identical with the internal diameter size of upper cover plate round mouth, and the lower end outer wall is provided with external thread, is screwed by internal thread and external thread between upper cover plate (11) and bottle stopper (10);
Bottle (1), the bottle end (2) and bottle stopper (10) by light transmission preferably transparent organic glass make;
Bottle (1) upper end outer wall is provided with groove, is inlaid with steel ring (7) in the groove, is fixed with the fixedly steel disc (8) that has aperture (6) on the steel ring (7).
2. according to the described device of claim 1, it is characterized in that: be the unlatching of convenient bottle stopper, be adhesive with the poly (methyl methacrylate) plate (3) of 6 dihedrals in bottle stopper (10) top, the poly (methyl methacrylate) plate (3) of bottle stopper (10) and 6 dihedrals constitutes a bottle cap, in order to reinforce the firm degree that engages between them, placed a bolt (9) at bottle stopper and 6 dihedral poly (methyl methacrylate) plate centers, the bolt lower end is connected with the suspension ring (4) that are used to hang the experiment biological sample.
3. according to the described device of claim 2, it is characterized in that:
On the upper cover plate of bottle, be the center of circle with the center of round mouth, be provided with annular groove in the periphery of round mouth, be placed with O-ring seal (5) in it; After upper cover plate and bottle stopper screwed togather, 6 dihedral poly (methyl methacrylate) plates were pressed on the O-ring seal.
4. according to the described device of claim 1, it is characterized in that: the fixedly steel disc (8) that has aperture is 1-6, and it evenly is fixed on the steel ring (7) by screw, has fixedly placed rope in the aperture (6) of steel disc (8).
5. according to the described device of claim 1, it is characterized in that: the lower sides of bottle (1) is provided with apopore;
Be connected with emulsion tube on the apopore, emulsion tube is provided with the clip that prevents that water from flowing out,
Perhaps insert one section plexi-glass tubular as rising pipe in apopore, rising pipe is provided with the switch of a ground.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100138321A CN101482552B (en) | 2008-01-11 | 2008-01-11 | In-situ measurement apparatus for macroscopic algae photosynthesis rate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100138321A CN101482552B (en) | 2008-01-11 | 2008-01-11 | In-situ measurement apparatus for macroscopic algae photosynthesis rate |
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| Publication Number | Publication Date |
|---|---|
| CN101482552A true CN101482552A (en) | 2009-07-15 |
| CN101482552B CN101482552B (en) | 2013-03-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100138321A Expired - Fee Related CN101482552B (en) | 2008-01-11 | 2008-01-11 | In-situ measurement apparatus for macroscopic algae photosynthesis rate |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107913749A (en) * | 2017-12-05 | 2018-04-17 | 佛山科学技术学院 | A kind of cuvette construction |
| CN109935149A (en) * | 2019-04-10 | 2019-06-25 | 陶洁敏 | A kind of photosynthesis experimental teaching unit |
| CN111830211A (en) * | 2020-07-30 | 2020-10-27 | 中国水产科学研究院南海水产研究所 | RS-based ocean primary productivity distribution visualization method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2004117215A (en) * | 2001-11-08 | 2005-03-10 | Анне Марие ФАНГЕР (DE) | METHOD AND DEVICE FOR DETERMINING THE VALUE OF REAL PHOTOSYNTHESIS IN PLANTS |
-
2008
- 2008-01-11 CN CN2008100138321A patent/CN101482552B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107913749A (en) * | 2017-12-05 | 2018-04-17 | 佛山科学技术学院 | A kind of cuvette construction |
| CN109935149A (en) * | 2019-04-10 | 2019-06-25 | 陶洁敏 | A kind of photosynthesis experimental teaching unit |
| CN111830211A (en) * | 2020-07-30 | 2020-10-27 | 中国水产科学研究院南海水产研究所 | RS-based ocean primary productivity distribution visualization method |
| CN111830211B (en) * | 2020-07-30 | 2021-05-04 | 中国水产科学研究院南海水产研究所 | RS-based ocean primary productivity distribution visualization method |
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|---|---|
| CN101482552B (en) | 2013-03-06 |
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Granted publication date: 20130306 Termination date: 20140111 |