CN101461539B - Method for preparing protein powder - Google Patents
Method for preparing protein powder Download PDFInfo
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- CN101461539B CN101461539B CN2009100452243A CN200910045224A CN101461539B CN 101461539 B CN101461539 B CN 101461539B CN 2009100452243 A CN2009100452243 A CN 2009100452243A CN 200910045224 A CN200910045224 A CN 200910045224A CN 101461539 B CN101461539 B CN 101461539B
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Abstract
The invention pertains to the field of food technology, specially relates to a preparation of protein powder. The invention further discloses a preparation of protein powder, which is characterized in adding probiotics into edible protein powder, anaerobic culturing in water medium, and carrying out proteolysis. By adding probiotics capable of generating prolease in the edible protein powder, which substrates or reinforces prolease in the food additives, the invention makes hydrolysis speed of the protein powder greatly increased in a determined period. The method is more convenient and economic then only adding prolease; the obtained protein powder is more abundant in nutrient, and easier for human body absorption.
Description
Technical field
The present invention relates to food technology field, be specifically related to a kind of preparation method of protein powder.
Background technology
Protein powder is as a kind of common diet nutrient; Be widely used in the health products trade; The effect of discovered in recent years protein powder is except providing necessary for human body amino acid; Its little peptide that under the protease effect, is decomposed to form also has some special biologically actives, and these little peptides are called as biologically active peptide.These polypeptide are so small to have only 2 amino acid whose pair of peptides; Also can arrive complicated long-chain or ring type polypeptide greatly; And often derive through glucosidesization, phosphorylation or acidylate; The discovery that in the adjusting of cell physiological and metabolic function, has important effect, particularly small peptide has become the exploitation focus of polypeptide drug and functional food additives.These little peptides as a kind of nutrition peptide malnutritive or digest and assimilate on problematic patient's the formula food, can substituted amino acid as the application of nitrogenous source.Proved that the efficient that dipeptides and tripeptides be absorbed by the body is high than amino acid, γ-Glu-Lyn strengthened in wheat gluten, to make bread be extraordinary Lyn source like people, added the polypeptide that constitutes by free phenylalanine etc. in the phenyl ketonuria patient meals of being everlasting.From cow's milk protein and soybean protein, separating the nutritional activities peptide that obtains is the focus of paying close attention to, some from phosphoeptides of casein and β-casomorphin as meals hardening agent and medicine in use.They have physiologically active widely, as: the absorption of immunological regulation, prophylaxis of hypertension, anticoagulation and adjusting mineral matter etc.Though the cow's milk peptide is had a large amount of research in external effect, and its important function in vivo it be unclear that so far.
Therefore and be unfavorable for the absorption of human body utilization albumen that contains in the common albumen powder all is to exist with macromolecular form, and the quantity of its amino acid monomer does not wherein contain the less little peptide of various molecular weight all greater than 100.So the various small peptides of interpolation in protein powder that people have strengthen the nutritive validity of albumen powder; Some protease of the interpolation that has; Like papain, trypsase etc., make albumen in human body, be easier to be hydrolyzed into various little peptides, to promote the absorption and the utilization of albumen powder.But because the protease or the cost that add are very high; Like chymotrypsin or kind is single, its hydrolysis site is single, can not reach effective decomposing macromolecular amount albumen and form the target of various little peptides; Like papain etc.; Have itself is just unstable, the oneself decomposes and loses activity easily, like trypsase etc.From the consideration of financial cost and hydrolysis effect, seek the substitute of these protease or the problem that reinforcing agent becomes a needs thinking.
Summary of the invention
The invention provides a kind of new protein powder preparation method, to substitute or to strengthen the effect of protease additive in the albumen powder.
For this reason, the preparation method of a kind of protein powder of the present invention is characterized in that this method comprises in the table egg white powder and has added probio that anaerobic is cultivated under the aqueous medium, carries out proteolysis.
Table egg white powder of the present invention is lactalbumin, soybean protein, casein etc.;
Said in certain embodiments probio comprises: by streptococcus thermophilus, lactobacillus bulgaricus, Bifidobacterium, lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus casei (Lactobacilluscasei) etc. separately or mix the bacterial classification of forming.
Bifidobacterium of the present invention comprises: bifidobacterium adolescentis (Bifidobacteriumadolescentis), bifidobacterium infantis (Bifidobacterium infantis), bifidobacterium longum (Bifidobacterium longum), bifidobacterium breve (Bifidobacterium breve) and bifidobacterium bifidum (Bifidobacterium bifidum).
In certain embodiments, the content of said probio is 0.4% of gross mass, and viable bacteria is measured greater than 10
8CFU/g;
In certain embodiments, said anaerobic cultivation is 25-37 ℃ of anaerobic stir culture 6-36 hour;
In certain embodiments, probio and hydrolysising protease are united the molecular weight that use can reduce albumen in the albumen powder more fast, and molecular weight is 10-25% less than 5000 daltonian little peptide conversion ratios;
The preferred papain of hydrolysising protease.
Can find to use separately the effect of papain hydrolysis very big-difference to be arranged in an embodiment of the present invention with using the effect of probio separately; Show that independent use probio is easy to produce molecular weight less than 5000 daltonian little peptides; And use papain not have this effect separately, but but be more or less the same on less than 10000 daltonian little peptide effects for forming molecular weight;
Can find in an embodiment of the present invention if papain and probio are united use; With the hydrolysis effect that strengthens protein greatly; Though for molecular weight less than 5000 daltonian little peptides or molecular weight less than 10000 daltonian little peptides, its result improves a lot;
Can also find in an embodiment of the present invention for different protein; The synergetic hydrolysis effect of papain and probio is different; If contain more how diffluent lactalbumin in the initial albumen powder; Compare and contain more not diffluent soybean proteins, its molecular weight can rise to 24.8% from 17.9% less than 5000 daltonian little hydrolase polypeptide conversion ratios;
Can also find to improve the content of albumen in the albumen powder in an embodiment of the present invention; Reduce the content of the former starch of fruit; Can reduce the conversion ratio of albumen powder hydrolysis; This maybe be relevant with the synergistic conversion capability of papain in the unit interval and probio, and protein concentration surpasses certain limit conversion capability and will descend, and its sign is exactly that conversion ratio reduces.
Useful achievement of the present invention: the present invention is through adding the probio that can produce protease in the table egg white powder; Play the effect that substitutes or strengthen protease in the food additives; Albumen powder hydrolysis rate is within a certain period of time increased greatly; Method of the present invention is more more convenient, more economical than only adding protease; Resulting albumen powder more abundant in nutrient helps absorption of human body more, and it shows as molecular weight can significantly increase less than 5000 daltonian little peptides.
The specific embodiment
Below in conjunction with specific embodiment, further illustrate the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Ratio and percentage are based on weight, unless stated otherwise.
Embodiment 1:
(the bacterium amount is＞10 to get soybean protein isolate 60g, the former starch 20g of fruit, polydextrose 10g, lactalbumin 9.6g, bifidobacterium infantis bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, bifidobacterium breve bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixes, and gets the dry powder 10g that mixes and is dissolved in the 1L sterile pure water, 37 ℃ of anaerobic stir culture 8 hours; Each sampling once before and after cultivating; Institute's sample thief went deposition in centrifugal 10 minutes under the 10000rpm condition, centrifugal supernatant is used molecular weight 10000 dalton, molecular weight 5000 daltonian ultrafiltration membrance filters respectively, got liquid under the filter membrane; Measure its protein content with the Lowwry method; Can find that bigger variation has taken place the protein concentration of cultivating liquid under the filter membrane of front and back, like liquid under the molecular weight 10000 daltonian films, its protein concentration rises to 2.1g/L by the 0.53g/L before cultivating; It is 22.4% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 1.1g/L by the 0.15g/L before cultivating, and it is 13.6% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 2:
Get soybean protein isolate 60g, the former starch 20g of fruit, polydextrose 10g, lactalbumin 10g, papain 0.01g (purity is greater than 80%) mixing; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ were stirred 8 hours, and each sampling once before and after cultivating; Institute's sample thief went deposition in centrifugal 10 minutes under the 10000rpm condition; Centrifugal supernatant is used molecular weight 10000 dalton, molecular weight 5000 daltonian ultrafiltration membrance filters respectively, get liquid under the filter membrane, measure its protein content with the Lowwry method; Liquid under the molecular weight 10000 daltonian films; Its protein concentration rises to 2.4g/L by the 0.48g/L before cultivating, and it is 27.4% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight, liquid under the molecular weight 5000 daltonian films; Its protein concentration rises to 0.75g/L by the 0.11g/L before cultivating, and it is 9.1% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 3:
(the bacterium amount is＞10 to get soybean protein isolate 60g, the former starch 20g of fruit, polydextrose 10g, lactalbumin 9.6g, papain 0.01g (purity is greater than 80%), bifidobacterium infantis bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, bifidobacterium breve bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixes; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ are stirred anaerobic cultivation 8 hours, and each sampling once before and after cultivating; Institute's sample thief carries out centrifugal membrane filtration equally according to front two examples to be handled; Liquid is measured its protein content with the Lowwry method under the film, liquid under the molecular weight 10000 daltonian films, and its protein concentration rises to 3.0g/L by the 0.46g/L before cultivating; It is 36.3% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 1.4g/L by the 0.15g/L before cultivating, and it is 17.9% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 4:
(the bacterium amount is＞10 to get soybean protein isolate 90g, polydextrose 3.65g, lactalbumin 6g, papain 0.01g (purity is greater than 80%), bifidobacterium bifidum bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, bifidobacterium longum bacterium powder
9CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixed; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ were stirred 8 hours, and each sampling once before and after cultivating; Institute's sample thief carries out centrifugal membrane filtration equally according to previous examples to be handled; Liquid is measured its protein content with the Lowwry method under the film, liquid under the molecular weight 10000 daltonian films, and its protein concentration rises to 2.6g/L by the 0.65g/L before cultivating; It is 20.3% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 1.5g/L by the 0.03g/L before cultivating, and it is 15.3% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 5:
(the bacterium amount is＞10 to get soybean protein isolate 90g, polydextrose 3.65g, lactalbumin 6g, papain 0.01g (purity is greater than 80%), bifidobacterium bifidum bacterium powder
9CFU/g) 0.1g, Lactobacillus casei bacterium powder (10
10CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixed; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ were stirred 8 hours, and each sampling once before and after cultivating; Institute's sample thief carries out centrifugal membrane filtration equally according to previous examples to be handled; Liquid is measured its protein content with the Lowwry method under the film, liquid under the molecular weight 10000 daltonian films, and its protein concentration rises to 2.3g/L by the 0.62g/L before cultivating; It is 17.5% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 1.7g/L by the 0.03g/L before cultivating, and it is 17.4% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 6:
(the bacterium amount is＞10 to get casein 30g, the former starch 20g of fruit, polydextrose 10g, lactalbumin 39g, papain 0.01g (purity is greater than 80%), bifidobacterium bifidum bacterium powder
9CFU/g) 0.1g, Lactobacillus casei bacterium powder (10
10CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixed; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ are stirred anaerobic cultivation 8 hours, and each sampling once before and after cultivating; Institute's sample thief carries out centrifugal membrane filtration equally according to previous examples to be handled; Liquid is measured its protein content with the Lowwry method under the film, liquid under the molecular weight 10000 daltonian films, and its protein concentration rises to 2.6g/L by the 0.45g/L before cultivating; It is 30.7% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 1.8g/L by the 0.06g/L before cultivating, and it is 24.8% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Embodiment 7:
(the bacterium amount is＞10 to get casein 30g, the former starch 20g of fruit, polydextrose 10g, lactalbumin 39g, papain 0.01g (purity is greater than 80%), lactobacillus bulgaricus bacterium powder
10CFU/g) 0.1g, Lactobacillus casei bacterium powder (10
10CFU/g) (the bacterium amount is for＞10 for 0.1g, lactobacillus acidophilus bacterium powder
10CFU/g) (the bacterium amount is for＞10 for 0.1g, streptococcus thermophilus
10CFU/g) 0.1g mixed; Get the dry powder 10g that mixes and be dissolved in the 1L sterile pure water, 37 ℃ are stirred anaerobic cultivation 8 hours, and each sampling once before and after cultivating; Institute's sample thief carries out centrifugal membrane filtration equally according to previous examples to be handled; Liquid is measured its protein content with the Lowwry method under the film, liquid under the molecular weight 10000 daltonian films, and its protein concentration rises to 2.9g/L by the 0.55g/L before cultivating; It is 33.6% less than 10000 daltonian little peptide conversion ratios that albumen powder is converted into molecular weight; Liquid under the molecular weight 5000 daltonian films, its protein concentration rises to 2.0g/L by the 0.08g/L before cultivating, and it is 27.4% less than 5000 daltonian little peptide conversion ratios that its albumen powder is converted into molecular weight.
Scope of the present invention does not receive the restriction of said specific embodiments, and said embodiment is only desired also to comprise the method and the component of functional equivalent in the scope of the invention as the single example of illustrating various aspects of the present invention.In fact, except content as herein described, those skilled in the art can easily grasp multiple improvement of the present invention with reference to the description of preceding text.Said improvement also falls within the scope of appended claims.
Claims (7)
1. the preparation method of a protein powder is characterized in that this method comprises in the table egg white powder and has added probio that anaerobic is cultivated under the aqueous medium, carries out proteolysis; Said probio is one of in streptococcus thermophilus, lactobacillus bulgaricus, Bifidobacterium, lactobacillus acidophilus, the Lactobacillus casei or their any combination;
Said table egg white powder is one of in lactalbumin, soybean protein or the casein or their any combination;
The content of said probio is 0.4% of gross mass, and viable bacteria is measured greater than 108CFU/g.
2. preparation method as claimed in claim 1 is characterized in that said Bifidobacterium comprises: one of in bifidobacterium adolescentis, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve or the bifidobacterium bifidum or their any combination.
3. preparation method as claimed in claim 1 is characterized in that it is 25-37 ℃ of anaerobic stir culture 6-36 hour that said anaerobic is cultivated.
4. preparation method as claimed in claim 1 is characterized in that also having added hydrolysising protease in the said table egg white powder.
5. preparation method as claimed in claim 1 is characterized in that said hydrolysising protease is a papain.
6. preparation method as claimed in claim 5, the consumption that it is characterized in that said papain is 0.01% of a gross mass.
7. like each described preparation method of claim 5-6, it is characterized in that the content 40%-85% of albumen in the said table egg white powder, the content 0-20% of the former starch of fruit.
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| CN2009100452243A CN101461539B (en) | 2009-01-13 | 2009-01-13 | Method for preparing protein powder |
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| CN101461539B true CN101461539B (en) | 2012-11-07 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102379359B (en) * | 2010-08-30 | 2013-09-25 | 内蒙古伊利实业集团股份有限公司 | Casein enzymatic hydrolysis method and hydrolysis product |
| CN102960603B (en) * | 2012-12-12 | 2014-02-26 | 山东龙力生物科技股份有限公司 | Nutrient protein powder with high digestive absorption function |
| CN104824664A (en) * | 2015-04-17 | 2015-08-12 | 安徽珠峰生物科技有限公司 | Probiotics-containing nutritious protein powder and preparation method thereof |
| CN108260790A (en) * | 2017-01-04 | 2018-07-10 | 永和食品（中国）股份有限公司 | A kind of nourishing albumen powder and preparation method thereof |
| CN107410662A (en) * | 2017-05-09 | 2017-12-01 | 江苏康德蛋业有限公司 | A kind of new food additive egg albumen powder and preparation method thereof |
| CN107280014A (en) * | 2017-08-02 | 2017-10-24 | 安徽金源药业有限公司 | It is a kind of to promote the compound probiotic protein powder of Instestinal motility |
| CN108354185A (en) * | 2018-04-11 | 2018-08-03 | 泉州妙吉堂健康管理有限公司 | A kind of enzyme food and preparation method thereof adjusting enteron aisle digestive function |
-
2009
- 2009-01-13 CN CN2009100452243A patent/CN101461539B/en active Active
Non-Patent Citations (1)
| Title |
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| 吴泽柱，曹龙奎.《粮油加工》.《水解玉米蛋白粉菌种的筛选研究》.2008,第×卷(第11期),92－96. * |
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Effective date of registration: 20170209 Address after: 10666 No. 201700 Shanghai city Qingpu District Songze Avenue Patentee after: BIOGROWING (SHANGHAI) Co.,Ltd. Address before: 201206 Pudong New Area Jinqiao Export Processing Zone, Nanjing Road, No. 999, Shanghai Patentee before: Shanghai Shuangjin Biological Technology Co.,Ltd. |
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Address after: 10666 Songze Avenue, Qingpu District, Shanghai, 201700 Patentee after: Biogrowing. Co.,Ltd. Address before: 10666 Songze Avenue, Qingpu District, Shanghai, 201700 Patentee before: BIOGROWING (SHANGHAI) Co.,Ltd. |