CN101439073B - Application of Chinese medicinal composition in preparing medicament for preventing and treating dysmnesia and improving memory - Google Patents
Application of Chinese medicinal composition in preparing medicament for preventing and treating dysmnesia and improving memory Download PDFInfo
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- CN101439073B CN101439073B CN2008101890452A CN200810189045A CN101439073B CN 101439073 B CN101439073 B CN 101439073B CN 2008101890452 A CN2008101890452 A CN 2008101890452A CN 200810189045 A CN200810189045 A CN 200810189045A CN 101439073 B CN101439073 B CN 101439073B
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Abstract
The invention discloses application of a traditional Chinese medicine composition in the preparation of a medicine for preventing and curing dysmnesia and improving memory. The traditional Chinese medicine composition consists of supercritical extracts and/or water extracts of any two of the raw materials of tuckahoe, large-head atractylodes rhizome and angelica, or consists of effective parts ofthe supercritical extracts and/or the water extracts.
Description
This application is dividing an application of following patent application: application number is 200510111694.7, and the applying date is December in 2005 20 days, and denomination of invention is " a kind of Chinese medicine composition is in the application for the treatment of cardiac and cerebral vascular diseases ".
Technical field
The present invention relates to a kind of purposes of Chinese medicine composition, particularly, relate to a kind of Chinese medicine composition and prevent and treat dysmnesia in preparation, improve the application in the medicine of remembering.
Background technology
On November 2nd, 2005, the patent of invention description CN1225250C of bulletin disclosed a kind of Chinese medicine composition of preventing and treating senile dementia, it mainly is made up of Poria, the Rhizoma Atractylodis Macrocephalae and polysaccharide, n-butanol extract and/or volatile oil effective site in the crude drug that is grouped into, perhaps mainly is made up of the polysaccharide in one or both crude drug that are selected from Poria, the Rhizoma Atractylodis Macrocephalae and the Radix Angelicae Sinensis, n-butanol extract and/or volatile oil effective site.This Chinese medicine composition can adopt the method for supercritical extraction and water extraction to make.
Above-mentioned Chinese medicine composition has the purposes of control senile dementia.The present invention has excavated the new medical usage of this Chinese medicine composition.For known drug, find that it also has the performance of the another kind of disease of treatment, thereby can treat another kind of disease that this medical usage invention second time is for satisfying the medical clinical demand and the market demand, and is significant.
In view of the important function of the medical usage second time, the new purposes of researching and developing above-mentioned Chinese medicine composition is significant.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of new purposes of Chinese medicine composition, promptly prevents and treats dysmnesia in preparation, improves the application in the medicine of remembering.
The invention provides a kind of Chinese medicine composition prevents and treats dysmnesia in preparation, improves the application in the medicine of remembering, described Chinese medicine composition is by the supercritical extract and/or the water extract of Poria 2-6 part, Rhizoma Atractylodis Macrocephalae 1-3 part and three kinds of crude drug of Radix Angelicae Sinensis 0.5-1.5 part, and perhaps supercritical extract and/or water extract effective site are formed.
For sake of convenience, with Poria of the present invention, the Rhizoma Atractylodis Macrocephalae, abbreviate FBD as when the Chinese medicine composition that is grouped into.Poria is called for short F, the Rhizoma Atractylodis Macrocephalae is called for short B, Radix Angelicae Sinensis abbreviation D.
FBD of the present invention can adopt following method to make: crude drug is ground into coarse powder, and through the carbon dioxide supercritical fluid extraction thing, medicinal residues obtain water extract after with water extraction afterwards; Perhaps crude drug is after supercritical extraction obtains supercritical extract, medicinal residues with water extraction after, add ethanol and precipitate when containing determining alcohol for 45-95%, obtain polysaccharide, supernatant is used n-butanol extraction after reclaiming ethanol, obtains n-butanol extract; Perhaps, described crude drug water decocts, and after aqueous extract concentrates, adds ethanol and precipitates when containing determining alcohol for 45-95%, obtains polysaccharide, and supernatant is used n-butanol extraction after reclaiming ethanol, obtains n-butanol extract; Polysaccharide and n-butanol extract are water extract effective site.
In described supercritical extract and water extract effective site, can add pharmaceutical carrier or dressing, make Chinese medicine preparation.The common dosage forms adjuvant can be selected for use, and disintegrating agent is as hydroxypropyl starch, hyprolose, carboxymethyl starch sodium, carboxymethylcellulose calcium, polyvinylpolypyrrolidone, cross-linking sodium carboxymethyl cellulose etc.; Filler is as lactose, sucrose, mannitol, microcrystalline Cellulose, dextrin, starch, calcium phosphate, calcium hydrogen phosphate, calcium sulfate, calcium carbonate, cyclodextrin, micropowder cellulose etc.; Wetting agent and binding agent are as ethanol, pregelatinized Starch, polyvidone, sodium carboxymethyl cellulose, hypromellose; Lubricant is as Pulvis Talci, stearic acid, magnesium stearate, calcium stearate, micropowder silica gel, hydrogenated vegetable oil, Macrogol 4000 and 6000; Wetting agent is as sodium lauryl sulphate, Tween 80.
The consumption of FBD of the present invention and the course of treatment can be done suitably to adjust according to the light and heavy degree of dosage form, patient's age, disease, but are generally oral 3 times of every day, each 2, with 1 month be a course of treatment, can use 1 course of treatment or longer time continuously.
In order to understand essence of the present invention better, will its new purposes in pharmaceutical field be described with pharmacological testing and the result of FBD of the present invention below:
Find in pharmacodynamic study: FBD consolidates in the bad influence research sodium nitrite induced mice memory, and FBD is to consolidating the bad good improvement effect that has by memory due to the sodium nitrite, and its action intensity is like stronger than huperzine A.The result shows that FBD can effectively prevent and treat dysmnesia, and improves memory, improves normal person's memory ability.
The invention has the advantages that: the present invention has excavated new medical application to known Chinese medicine composition FBD, has opened up a new application.FBD safety non-toxic of the present invention, pharmacological action is strong, is indicating well prospect in medicine.FBD preparation technology of the present invention is simple, and can be made into various peroral dosage forms, as tablet, capsule etc.FBD of the present invention consolidates the bad good improvement effect that has to memory.These effects help preventing and treating dysmnesia, improve memory.
The specific embodiment
Below by test the example beneficial effect of the present invention is further elaborated:
Test routine FBD bad influence is consolidated in sodium nitrite induced mice memory
Principle: have the medicine that improves learning and memory can reduce the memory of sodium nitrite induced mice and consolidate the number of animals that bad mice was shocked by electricity after 24 hours, the wrong number in the 3min reduces, and shortening incubation period of jumping off platform for the first time.
1 experiment purpose: observe FBD to consolidate the bad not improvement effect that has by the memory of sodium nitrite induced mice.
2 animal strains, sex, body weight and source are all with 1.2
3 medicines
3.1 given the test agent
Title: Guiling tablets (FBD) source: Shanghai Traditional Chinese Medicine Pharmaceutical Technology Co., Ltd
Character: peony tablet lot number: 040904
Specification: 0.48g/ sheet
3.2 positive control sample
Title: aspirin source: Shanghai XinYi BaiLuDa Medicine Co., Ltd
Specification: 25mg/ sheet character: white enteric coatel tablets lot number: 030503
3.3 other reagent
Title: sodium nitrite (NaNO
2) character: white crystalline solid specification: analytical pure
Source: Chemical Reagent Co., Ltd., Sinopharm Group's lot number: 20040722
The grouping of 4 animals is by the grouping of random digit method, every group of 10 animals.
The preparation of 5 medicinal liquids
6 instruments
Title: mice diving tower monitor
Model: YLS-2T type
Source: Shandong Academy of Medical Sciences equipment station
7 experimental techniques
1st, 2 groups of mice orally give N.S, the 3rd group of (positive drug group) orally give huperzine A 66.7 μ g/kg, fbd organizes oral administration.1 time/day, 0.2ml/10g body weight, one week of successive administration.After the last administration 1 hour, lumbar injection scopolamine 2mg/kg behind the 30min put into mice diving tower instrument endoadaptation 5min.Then the energising, the normal reaction after animal is shocked by electricity be the rebound platform to hide noxious stimulation, most animals may be once more or is repeatedly skipped on the copper grid, the platform that snaps back again after being shocked by electricity is so trained 5min, writes down the number of times that every Mus is shocked by electricity.Resurvey after 24 hours, the number of animals that record is shocked by electricity is jumped off the incubation period of platform and the wrong sum in the 3min for the first time.
8 results
Sodium nitrite causes anoxic dysmnesia, so the model group mice has obviously shortened the incubation period of jumping off platform for the first time; Huperzine A has prolonged the incubation period that mice jumps off platform for the first time, compares P＜0.05 with model group.The number of animals that is shocked by electricity also significantly reduces (compare with model group, the P value all＜0.01) with the interior errors number of 3min; FBD 4.6,2.3g/kg all significantly prolonged incubation period of jumping off platform for the first time, the number of animals that shocked by electricity (is compared with model group with the errors number in the 3min, the P value all＜0.01), and effect shows that like being eager to excel than huperzine A FBD can improve by the dysmnesia due to the cerebral anoxia.1.15g/kg FBD improve the effect not obvious (table 18) of above-mentioned two indexs.
(x ± SD) expression, the data difference statistics adopts one factor analysis of variance (ANOVA) or X to data with mean+SD
2Check, group difference is judged with P＜0.05 or P＜0.025.
Table 18.6 group mice is consolidated bad result of the test (n=10, x ± SD) to memory
#P＜0.025 ##P＜0.01 is compared with negative control group;
* P＜0.05 * * P＜0.01 is compared with model group
9 conclusions
With above-mentioned two amnemonic result of the tests, FBD is to consolidating the bad good improvement effect that has by memory due to the sodium nitrite, and its action intensity is like stronger than huperzine A.The result shows that FBD can effectively prevent and treat dysmnesia, and the bad good improvement effect that has is consolidated in memory.
The invention will be further elaborated by the following examples:
Embodiment 1
Get Poria 25g, Rhizoma Atractylodis Macrocephalae 12g, Radix Angelicae Sinensis 7g, after the mixing, water decocts, and after aqueous extract concentrates, adds ethanol precipitation, get polysaccharide (2.3g), supernatant is used n-butanol extraction after reclaiming ethanol, gets n-butanol portion (0.71g), merges water extract effective site (polysaccharide and n-butanol extract), after adding appropriate amount of auxiliary materials, be pressed into tablet.
Embodiment 2
Get Poria 50g, Rhizoma Atractylodis Macrocephalae 40g, Radix Angelicae Sinensis 20g, after the mixing, the CO2 supercritical extraction extracts volatile oil (2.5ml), and it is 10.0MP that extractor pressure is extracted in control, and temperature is 35 ℃, and extraction time is 2 hours.Medicinal residues with water extraction after, adding ethanol to the alcohol amount of containing is 90%, precipitation obtains total polysaccharides (4.2g), supernatant use n-butanol extraction after reclaiming ethanol, merges volatile oil, total polysaccharides and n-butanol extract (7.5g) add appropriate amount of auxiliary materials, capsule is recorded in granulation.
Embodiment 3
Get Poria 40g, Rhizoma Atractylodis Macrocephalae 20g, Radix Angelicae Sinensis 10g, after the mixing, the CO2 supercritical extraction, control extractor pressure is 12.0MP, and temperature is 35 ℃, and extraction time is 2 hours, obtains supercritical extract.Medicinal residues with water extraction after, adding ethanol to the alcohol amount of containing is 85%, precipitation obtains total polysaccharides, supernatant use n-butanol extraction after reclaiming ethanol, merges supercritical extract, total polysaccharides and n-butanol extract add appropriate amount of auxiliary materials, capsule is recorded in granulation.
Embodiment 4
Get Poria 40g, Rhizoma Atractylodis Macrocephalae 20g, Radix Angelicae Sinensis 12g, after the mixing,, carry out the CO2 supercritical extraction and extract volatile oil (1.64ml) with the ethanol entrainer, medicinal residues with water extraction after, add ethanol precipitation, obtain total polysaccharides (2.74g), supernatant is used n-butanol extraction (4.9g) after reclaiming ethanol, merge volatile oil, total polysaccharides and n-butanol extract add appropriate amount of starch, granulate, record 16 of capsules.Taking dose: took 2 times each 2 on the 1st.
Embodiment 5
Get Poria 50g, Rhizoma Atractylodis Macrocephalae 25g, Radix Angelicae Sinensis 15g, after the mixing, water decocts, and after aqueous extract concentrates, adds ethanol precipitation, get polysaccharide (4.77g), supernatant is used n-butanol extraction after reclaiming ethanol, gets n-butanol portion (1.46g), merges polysaccharide and n-butanol extract, after adding appropriate amount of auxiliary materials, make 18 in tablet.Taking dose: took 2 times each 2 on the 1st.
Embodiment 6
Get Poria 50g, Rhizoma Atractylodis Macrocephalae 40g, Radix Angelicae Sinensis 20g, after the mixing, the CO2 supercritical extraction extracts volatile oil (2.5ml), and it is 10.0MP that extractor pressure is extracted in control, and temperature is 35 ℃, and extraction time is 2 hours.Medicinal residues with water extraction after, obtain water extract, merge volatile oil and water extract, add appropriate amount of auxiliary materials, compacting in flakes.
Claims (1)
1. a Chinese medicine composition is prevented and treated dysmnesia in preparation, is improved the application in the medicine of remembering, described Chinese medicine composition is by the supercritical extract and/or the water extract of Poria 2-6 part, Rhizoma Atractylodis Macrocephalae 1-3 part and three kinds of crude drug of Radix Angelicae Sinensis 0.5-1.5 part, and perhaps supercritical extract and/or water extract effective site are formed; This Chinese medicine composition adopts following method to make: crude drug is ground into coarse powder, obtains supercritical extract through carbon dioxide supercritical fluid extraction, medicinal residues obtain water extract after with water extraction afterwards; Perhaps crude drug is after supercritical extraction obtains supercritical extract, medicinal residues with water extraction after, add ethanol and precipitate when containing determining alcohol for 45-95%, obtain polysaccharide, supernatant is used n-butanol extraction after reclaiming ethanol, obtains n-butanol extract; Perhaps, described crude drug water decocts, and after aqueous extract concentrates, adds ethanol and precipitates when containing determining alcohol for 45-95%, obtains polysaccharide, and supernatant is used n-butanol extraction after reclaiming ethanol, obtains n-butanol extract; Polysaccharide and n-butanol extract are water extract effective site.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101890452A CN101439073B (en) | 2005-12-20 | 2005-12-20 | Application of Chinese medicinal composition in preparing medicament for preventing and treating dysmnesia and improving memory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101890452A CN101439073B (en) | 2005-12-20 | 2005-12-20 | Application of Chinese medicinal composition in preparing medicament for preventing and treating dysmnesia and improving memory |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101116947A Division CN100558371C (en) | 2005-12-20 | 2005-12-20 | A kind of Chinese medicine composition is prevented and treated application in the medicine of apoplexy in preparation |
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| Publication Number | Publication Date |
|---|---|
| CN101439073A CN101439073A (en) | 2009-05-27 |
| CN101439073B true CN101439073B (en) | 2011-01-19 |
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| CN2008101890452A Expired - Fee Related CN101439073B (en) | 2005-12-20 | 2005-12-20 | Application of Chinese medicinal composition in preparing medicament for preventing and treating dysmnesia and improving memory |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1225250C (en) * | 2003-07-31 | 2005-11-02 | 中国药科大学 | Efficacious parts compositions for prevention and cure of senile dementia and preparation method |
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2005
- 2005-12-20 CN CN2008101890452A patent/CN101439073B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1225250C (en) * | 2003-07-31 | 2005-11-02 | 中国药科大学 | Efficacious parts compositions for prevention and cure of senile dementia and preparation method |
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| CN101439073A (en) | 2009-05-27 |
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Granted publication date: 20110119 Termination date: 20191220 |