CN101433190A - 用蜂蜜残渣促进宿轮虫生长的方法 - Google Patents

用蜂蜜残渣促进宿轮虫生长的方法 Download PDF

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CN101433190A
CN101433190A CNA2008102039710A CN200810203971A CN101433190A CN 101433190 A CN101433190 A CN 101433190A CN A2008102039710 A CNA2008102039710 A CN A2008102039710A CN 200810203971 A CN200810203971 A CN 200810203971A CN 101433190 A CN101433190 A CN 101433190A
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habrotrocha
honey residue
residue
culture
growth
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CN101433190B (zh
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丁国际
杨芳芳
沈成媛
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Nantong Kangde Biological Product Co., Ltd.
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University of Shanghai for Science and Technology
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Abstract

本发明为一种用蜂蜜残渣促进宿轮虫生长的方法，属水污染控制技术领域。通过投加富含活性物质黄酮类和萜烯类等的蜂蜜残渣而达到有效促进宿轮虫的生长的目的。本发明是在用CaCO3、磷酸盐缓冲液和蒸馏水配制而成的轮虫培养液中，投加蜂蜜残渣，以葡萄球菌为轮虫食物，在25℃光照培养箱中进行轮虫的培养。本发明的选用蜂蜜残渣这种食品生产废料作为添加剂用于促进宿轮虫的生长，一方面其对宿轮虫的促生长作用非常显著，可使宿轮虫对数增长期内的种群增长率达0.87d－1，远高于不投加蜂蜜残渣时的0.55d－1；另一方面可使蜂蜜残渣变废为用，实现资源的循环利用，也使未来实际运用到污水处理中时更经济。

Description

用蜂蜜残渣促进宿轮虫生长的方法
技术领域
本发明属于水污染控制技术领域，具体涉及一种用生物活性物质促进宿轮虫(Habrotrocha sp.)生长的方法。通过投加富含活性物质的蜂蜜残渣而达到有效促进宿轮虫的生长的目的。
背景技术
宿轮虫是一种在基质(活性污泥、生物膜)上周丛生活、以悬浮颗粒为食物的小型后生动物，它对减少污水处理系统中的悬浮固体并提高透视度有很大贡献，但其在污水处理装置中的密度却不高，因此，设法促进宿轮虫的生长从而提高其密度对污水处理具有非常重要的意义。
生物活性物质是一类植物或动物内源性物质，对生物机体有特定的活性功能。目前生物活性物质主要用于医疗卫生、健康保健等方面，而用于如轮虫这样的小型后生动物的研究还很少。当前的研究仅发现几种生育酚化合物可增加宿轮虫的寿命和生育力，但对污水处理经济成本过高。因此寻找一种经济有效的生物活性物质成为解决宿轮虫在污水处理装置中的密度的关键性问题。
蜂蜜残渣是提取蜂蜜后剩余的废渣，内含很多蜂胶和花粉，它们中含有黄酮类、萜烯类、维生素和其它生物活性物质。目前利用蜂蜜残渣来促进宿轮虫的生长尚属首例，其对资源的循环利用也有重要的意义。
发明内容
本发明的目的是提供一种有效促进宿轮虫生长的方法。
本发明是通过投加富含活性物质的蜂蜜残渣，使宿轮虫的生长得到显著促进；同时可使蜂蜜残渣变废为用，实现资源的循环利用。
一种用蜂蜜残渣促进宿轮虫生长的方法，其特征在于具体步骤如下：
a.配制轮虫的培养液，其配比为：0.06～1.00g CaCO3，6～8mL 1M的磷酸盐缓冲液(pH＝6.5)，加入蒸馏水配至1000mL，煮沸并灭菌；
b.用肉汤培养基大量培养葡萄球菌(Staphylococcus)，经离心后作为轮虫食物；
c.预培养：从污水中采集宿轮虫，分离后置入口径为60mm的培养皿中，加入8mL步骤a的培养液、步骤b的葡萄球菌106～108cells·mL-1、蜂蜜残渣5μg，实验室内在25℃光照培养箱中进行培养，预培养时间持续7天；
d.在培养皿中放入6个从预培养的培养皿中随机挑取的轮虫幼体(不带卵轮虫)，加入8mL的轮虫培养液，葡萄球菌106～108cells·mL-1，再加入3～15μg的蜂蜜残渣，每天向培养皿中补充与初始日培养皿中含同等浓度的蜂蜜残渣及葡萄球菌的轮虫培养液；
e.每隔24小时计数培养容器中成活宿轮虫的数量，培养实验11天后结束。
本发明的主要内容为：在口径为60mm的培养皿中，内含8mL用CaCO3、磷酸盐缓冲液和蒸馏水配制而成的轮虫培养液，投加不同量的蜂蜜残渣，以葡萄球菌为轮虫食物，葡萄球菌的投加量为106～108cells·mL-1；在25℃光照培养箱中进行轮虫的培养。培养时间为11天，这时大多数轮虫的增长速率开始下降。每隔24小时计数培养容器中成活宿轮虫的数量。试验周期内每天向培养皿中补充的轮虫培养液为：与初始日每个培养皿中含同等浓度的蜂蜜残渣及葡萄球菌。
本发明的特点是选用蜂蜜残渣这种食品生产废料作为添加剂用于促进宿轮虫的生长，一方面其对宿轮虫的促生长作用非常显著，可使宿轮虫对数增长期内的种群增长率达0.87d-1，远高于不投加蜂蜜残渣时的0.55d-1；另一方面可使蜂蜜残渣变废为用，实现资源的循环利用，也使未来实际运用到污水处理中时更经济。
本发明制备的宿轮虫可用于减少污水处理系统中的悬浮固体并提高污水透视度。
具体实施方式
本发明的实施例是：
(1)配制轮虫的培养液，其配比为：0.06～1.00g CaCO3，6～8mL 1M的磷酸盐缓冲液(pH＝6.5)，加入约1000mL的蒸馏水，煮沸并灭菌；
(2)用肉汤培养基大量培养葡萄球菌，经离心后作为轮虫食物；
(3)预培养：从上海市曲阳污水处理厂好氧工艺段污水中采集宿轮虫，分离后置入口径为60mm的培养皿中，加入8mL步骤(1)的培养液、步骤(2)的葡萄球菌106～108cells·mL-1、蜂蜜残渣5μg，实验室内在25℃光照培养箱中进行培养，预培养时间持续7天；
(4)在每个培养皿中放入6个从预培养的培养皿中随机挑取的轮虫幼体(不带卵轮虫)，加入8mL的轮虫培养液，葡萄球菌106～108cells·mL-1，再分别加入0.0、4.5～5.0、7.0～7.5、9.5～10.0、12.0～12.5、14.0～14.5μg的蜂蜜残渣，每个实验做三个重复，其中投加蜂蜜残渣量0.0μg的设为控制组，每天向培养皿中补充与初始日每个培养皿中含同等浓度的蜂蜜残渣及葡萄球菌的轮虫培养液；
(5)每隔24小时计数培养容器中成活宿轮虫的数量，培养实验11天后结束，这时大多数轮虫的增长速率开始下降。
本实施例中宿轮虫的促生长作用非常显著，可使宿轮虫对数增长期内的种群增长率达0.87d-1，远高于对比例中不投加蜂蜜残渣时的0.55d-1。

Claims (1)

1.一种用蜂蜜残渣促进宿轮虫生长的方法，其特征在于该方法具体步骤如下：
a.配制轮虫的培养液，将0.06～1.00g CaCO3，6～8mL1M的磷酸盐缓冲液(pH＝6.5)，加入蒸馏水配至1000mL，煮沸并灭菌；
b.用肉汤培养基大量培养葡萄球菌，经离心后作为轮虫食物；
c.预培养：从污水中采集宿轮虫，分离后置入口径为60mm的培养皿中，加入8mL步骤a的培养液、步骤b的葡萄球菌106～108cells·mL-1、蜂蜜残渣5μg，实验室内在25℃光照培养箱中进行培养，培养时间持续7天；
d.在培养皿中放入6个从预培养的培养皿中随机挑取的轮虫幼体(不带卵轮虫)，加入8mL的轮虫培养液，葡萄球菌106～108cells·mL-1，再加入3～15μg的蜂蜜残渣，每天向培养皿中补充与初始日培养皿中含同等浓度的蜂蜜残渣及葡萄球菌的轮虫培养液；
e.每隔24小时计数培养容器中成活宿轮虫的数量，培养实验11天后结束。
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Cited By (4)

* Cited by examiner, † Cited by third party
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CN103141441A (zh) * 2012-10-23 2013-06-12 中国水产科学研究院东海水产研究所 一种干酵母和南极大磷虾粉培养轮虫的方法
CN104543586A (zh) * 2015-01-26 2015-04-29 安徽师范大学 一种四角平甲轮虫（Platyias quadricornis）快速繁殖培养基
CN105638581A (zh) * 2016-03-03 2016-06-08 上海大学 一种旋轮虫的培养基及培养方法
CN113816498A (zh) * 2021-10-29 2021-12-21 上海大学 一种投加蛭形轮虫提高废水生物处理效能的方法

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GB8531253D0 (en) * 1985-12-19 1986-01-29 English Clays Lovering Pochin Clay minerals
CN1989803A (zh) * 2005-12-26 2007-07-04 中国水产科学研究院东海水产研究所 轮虫培养中杀灭桡足类的方法

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103141441A (zh) * 2012-10-23 2013-06-12 中国水产科学研究院东海水产研究所 一种干酵母和南极大磷虾粉培养轮虫的方法
CN103141441B (zh) * 2012-10-23 2014-06-04 中国水产科学研究院东海水产研究所 一种干酵母和南极大磷虾粉培养轮虫的方法
CN104543586A (zh) * 2015-01-26 2015-04-29 安徽师范大学 一种四角平甲轮虫（Platyias quadricornis）快速繁殖培养基
CN105638581A (zh) * 2016-03-03 2016-06-08 上海大学 一种旋轮虫的培养基及培养方法
CN113816498A (zh) * 2021-10-29 2021-12-21 上海大学 一种投加蛭形轮虫提高废水生物处理效能的方法

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