CN101424690A - Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications - Google Patents
Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications Download PDFInfo
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- CN101424690A CN101424690A CNA2007101765922A CN200710176592A CN101424690A CN 101424690 A CN101424690 A CN 101424690A CN A2007101765922 A CNA2007101765922 A CN A2007101765922A CN 200710176592 A CN200710176592 A CN 200710176592A CN 101424690 A CN101424690 A CN 101424690A
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Abstract
The invention discloses an agent plate for testing hepatocarcinoma and a preparation method and the application thereof. The agent plate is composed of a rigid plate and an agent strip attached to the rigid plate, and the agent strip is formed by sequentially and closely splicing absorbent filter paper, a pyroxylin membrane, a glass fibre membrane and a sample adsorption glass fibre membrane from top to bottom, wherein a gold-labeled anti-mouse AFP monoclonal antibody is absorbed by the glass fibre membrane; an agglutinin coated detection line is arranged at one end of the pyroxylin membrane, which is adjacent to the glass fibre membrane, and a goat anti mouse IgG coated contrast line is arranged at the other end which is adjacent to the absorbent filter paper; and the lower end of the sample adsorption glass fibre membrane is provided with a row of loading holes. The agent plate for testing hepatocarcinoma has the advantages that a fluorescence microscope, an Elisa tester and other expensive instruments are not needed, and the agent plate is much more suitable for field test; the agent plate is safer since radioactive isotopes, TMB and other harmful substances or chemical substances are not needed in testing processes; test results can be preserved for a long time; and the operation of the agent plate is easy and fast, and the agent plate can be operated by a single person.
Description
Technical field
The present invention relates to a kind of agent plate that detects disease, relate in particular to a kind of agent plate, its preparation method and application that can accurate detection liver cancer, belong to biomedical sector.
Background technology
Because alpha-fetoprotein (AFP) has special relatively diagnostic value to hepatocellular carcinoma, therefore in the early detection of wide clinical application in hepatocellular carcinoma.But long-term clinical research shows, there is certain limitation in AFP in the diagnosis of liver cancer in early days, specific as follows: a specificity is poor: except that liver cancer, some other disease also can cause the rising of AFP in the serum, in particularly about 20%-40% chronic hepatitis patient and 20%-50% liver cirrhosis patient serum AFP rising phenomenon is arranged also, and liver cancer does not take place in these patients, chronic hepatitis or cirrhosis behind acute exacerbation in the serum AFP concentration also can reach a high level, even surpass 1000ng/ml.The 2nd, loss height: in order to improve specificity, differentiate good pernicious hepatopathy, existing examination criteria regulation: regulation AFP 〉=400ng/ml is the liver cancer-specific index, but, therefore only detect the omission that AFP causes a large amount of early liver cancer patients owing to have only patient AFP 〉=400ng/ml of 25.7% in the early liver cancer.
The glycosyl difference that the alpha-fetoprotein that the different lesions tissue produces contains, the alpha-fetoprotein that contains different glycosyls becomes the AFP heteroplasmon.The glycosyl that AFP is different is different with the compatibility of plant-derived agglutinin, AFP can be divided into AFP-L1, AFP-L2, AFP-L3 whereby.AFP-L1 mainly is present in the optimum hepatopathy, and AFP-L2 is from the pregnant woman, and AFP-L3 is an agglutinin mating type, by primary carcinoma of liver HCC cell peculiar.Clinical research finds that AFP-L3 is the high degree of specificity index of primary carcinoma of liver HCC, is the diagnosing cancer of liver mark of new generation of generally acknowledging.Studies show that, liver cancer AFP-L3 positive rate is 61%-86%, non-liver cancer patient positive rate is 1.6%-5%, adopt AFP-L3 can significantly improve the accuracy rate of the total AFP of simple detection, and have using value at aspects such as the grade malignancy of assessment tumour, the recurrence of pointing out tumour and treatments to diagnosing cancer of liver as the detection index of liver cancer.
Summary of the invention
Technical matters to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of agent plate that can accurate detection liver cancer is provided, and has highly sensitive, high specificity, safety non-pollution, quick, easy and simple to handle, low cost and other advantages.
Technical matters to be solved by this invention is achieved through the following technical solutions:
A kind of agent plate that detects liver cancer is made up of hard plate and the reagent strip that is attached on the hard plate; Described reagent strip is according to top-down order, successively by absorbent filter, nitrocellulose filter, be adsorbed with golden mark mouse-anti AFP monoclonal antibody glass fibre membrane and inhale sample glass fibre film close and be stitched together and form; Wherein, described nitrocellulose filter has a detection line that is formed by the agglutinin bag at the end near glass fibre membrane, at the other end near absorbent filter a control line that is formed by the sheep anti-mouse igg bag is arranged; There is row's well the lower end of described suction sample glass fibre membrane (promptly at the end away from the glass fibre membrane that is fixed with golden mark goat-anti AFP monoclonal antibody).
The described glass fibre membrane preparation method that is adsorbed with freeze-drying gold mark mouse-anti AFP monoclonal antibody is as follows: will evenly be added on the glass fibre membrane behind the mouse-anti AFP monoclonal anti body and function colloidal gold solution mark, adopt cryodesiccated mode dry more than 12 hours, promptly.
Described hard plate can be a hard plastic board, also can the hard plank or cardboard etc., as long as reagent strip can be fixed and had the material of certain rigidity and intensity, all can be used as hard plate of the present invention, and be preferably hard plastic board.
Described nitrocellulose filter also can be replaced by the film of similarities such as nylon membrane.
Wherein, the absorbent filter of forming reagent strip, nitrocellulose filter, be adsorbed with the glass fibre membrane of golden mark mouse-anti AFP monoclonal antibody and the area separately or the size of suction sample glass fibre membrane and all do not have special requirement, as long as under the prerequisite that satisfies above-mentioned splicing order, those skilled in the art can be as required or actual conditions the area of various films is adjusted, all can realize the present invention, its technique effect is also without any substantial difference, but, in order more to help convenient preparation and practical application, also in order to reduce production costs, the specification of described reagent strip is preferably 60 (length) * 4 (wide) mm simultaneously
2, the specification of described nitrocellulose filter is preferably 20 (length) * 4 (wide) mm
2, the glass fibre membrane of described freeze-drying gold mark mouse-anti AFP monoclonal antibody and the specification separately of suction sample glass fibre membrane are preferably 7 (length) * 4 (wide) mm
2, as for the specification of absorbent filter, its extent of elasticity is bigger, and the specification or the area of visual other three kinds of films are adjusted.
Used reagent among the present invention, for example mouse-anti AFP monoclonal antibody, sheep anti-mouse igg, collaurum raw material, agglutinin etc. all can be bought from commercial channels and obtain.
To be solved by this invention another provides a kind of method for preparing above-mentioned detection liver cancer agent plate.
Another technical matters to be solved by this invention is achieved through the following technical solutions:
A kind of method for preparing the agent plate that detects liver cancer comprises:
(1) preparation is adsorbed with the glass fibre membrane (gold mark glass fibre membrane) of freeze-drying gold mark mouse-anti AFP monoclonal antibody: will evenly be sprayed onto on the glass fibre membrane behind the mouse-anti AFP monoclonal anti body and function colloidal gold solution mark, and freeze drying, promptly;
(2) preparation contains the nitrocellulose filter of detection line and control line: after sheep anti-mouse igg antibody, agglutinin dilution, put respectively on 2 straight lines that parallel that nitrocellulose filter finishes in advance, dry, promptly get (sheep anti-mouse igg antibody of putting on the nitrocellulose filter forms detection line, and the agglutinin of putting on the nitrocellulose filter forms control line);
(3) nitrocellulose filter of absorbent filter with close detection line one end spliced mutually, the other end of nitrocellulose filter splices mutually with gold mark glass fibre membrane, and the other end of again gold being marked glass fibre membrane splices mutually with suction sample glass fibre membrane, obtains reagent strip; Fix or attach to reagent strip on the hard plate; Carrying out cutting or slitting according to needed specification, promptly.
Preferably, in the step (1), the described cryodesiccated time is preferably (more than 12 hours);
In the step (2), with sheep anti-mouse igg antibody, agglutinin respectively according to 0.75 μ l/cm
2Amount put on 2 straight lines that parallel that nitrocellulose filter finishes in advance; Described drying is preferably dry more than 12 hours;
In the step (3), at first gold being marked glass fibre membrane, to be cut into width be 7.0mm, and absorbent filter is cut into 24.0mm, inhales the sample glass fibre membrane and be cut into 15.0mm; The nitrocellulose filter of absorbent filter with close detection line one end spliced mutually, and wherein, nitrocellulose filter 1.0mm is pressed at the absorbent filter edge; The other end of nitrocellulose filter splices mutually with gold mark glass fibre membrane, and wherein nitrocellulose filter 1.0mm is pushed down at the edge of gold mark glass fibre membrane; Inhale the sample glass fibre membrane and be pasted on gold mark glass fibre membrane lower end, push down gold mark glass fibre membrane 2.0mm.
The present invention detects the agent plate of liver cancer and carries out clinical detection according to the principle that colloidal gold immunochromatographimethod detects, when test, sample to be tested (whole blood or serum) is added dropwise in the well of the lower end of inhaling the sample glass fibre membrane, liquid sample begins to move to test strips top owing to chromatographic theory, when tested sample runs into dry gold mark AFP antibody on glass fibre, it is dissolved, if there is AFP-L3 to exist in the sample, AFP-L3 in the sample at first marks the AFP antibodies with gold, the formation immune complex moves up, and continuation up moves to the detection line of nitrocellulose membrane, since AFP-L3 can with agglutinin generation specific bond, that is to say that the agglutinin that is coated on the detection line catches, form " sandwich " sandwich complex of " AFP-L3-agglutinin in golden mark AFP antibody-sample ", on detection line, form the red or mauve precipitation line of a dark color or light color.If there is not AFP-L3 in the sample, can not form compound with the agglutinin that is coated on the detection line, just there is not red or purplish red colo(u)r streak to occur on the detection line.Sample and gold mark AFP mixtures of antibodies continue up to move to control line, the sheep anti-mouse igg that is coated on the control line will be marked the AFP antibodies together with gold, on control line, form a redness or purplish red colo(u)r streak, show that this test strips is effective, show that reaction is invalid if control line redfree or purplish red colo(u)r streak occur, the test strips that should more renew this moment detects again.
It is as follows that the present invention detects the examination criteria of liver cancer agent plate:
Positive: dark or light red or purplish red colo(u)r streak appears in detection line, and red or purplish red colo(u)r streak appear in control line.
Negative: line does not appear in detection line, and red or purplish red colo(u)r streak appear in control line.
Invalid test: red or purplish red colo(u)r streak do not appear in control line, and this moment, no matter whether detection line invalid test occurred being, and the test strips that should more renew this moment detects again.The main cause that causes invalid experiment have the preservation condition of test strips expire, test strips defective (as make moist, high temperature etc.), experimental implementation error etc.
The present invention detects the liver cancer agent plate and has following advantage: 1. do not need expensive instruments such as fluorescent microscope, enzyme mark detector, more be applicable to on-the-spot the detection; 2. there is not the participation of objectionable impuritiess such as radioactive isotope, TMB, safer; 3. experimental result and gold mark probe can long preservation; 4. fast simple, can single part of operation.In a word, the present invention have sensitivity, special, easy and simple to handle, fast, do not need advantages such as instrument and equipment and other reagent.
Description of drawings
Fig. 1 the present invention detects liver cancer agent plate synoptic diagram;
A: the present invention detects the front elevation of liver cancer agent plate;
B: the present invention detects the rip cutting figure of liver cancer agent plate.
Drawing reference numeral explanation: 1: absorbent filter; 2: the nitrocellulose filter (NC) that is fixed with agglutinin and sheep anti-mouse igg; 3: the glass fibre that is fixed with golden mark goat-anti AFP monoclonal antibody; 4: inhale the sample glass fibre; 5: the control line that is coated with sheep anti-mouse igg; 6: the detection line that is coated with agglutinin; 7: the rigid plastic base plate.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
Embodiment 1 the present invention detects the preparation of liver cancer agent plate
One, test material and source
1 mouse-anti AFP monoclonal antibody
Mouse-anti AFP monoclonal antibody is available from tumour cell engineering National Laboratory of Xiamen University.Use ultraviolet spectrophotometer to measure protein concentration and be not less than 2.0mg/ml; With SDS-PAGE electrophoresis detection purity greater than 90%; Measure antibody titer with the ELISA indirect method, when being diluted to 1.0mg/ml, it is tired and is not less than 1: 256.
2 sheep anti-mouse iggs
Sheep anti-mouse igg antibody is available from tumour cell engineering National Laboratory of Xiamen University.Use ultraviolet spectrophotometer to measure protein concentration and be not less than 4.0mg/ml; With SDS-PAGE electrophoresis detection purity greater than 90%; Measure antibody titer with the ELISA indirect method, when being diluted to 1.0mg/ml, it is tired and is not less than 1: 128.
3 agglutinins
Agglutinin is available from tumour cell engineering National Laboratory of Xiamen University.Use ultraviolet spectrophotometer to measure protein concentration and be not less than 4.0mg/ml; With SDS-PAGE electrophoresis detection purity greater than 90%; Concentration is under the 3.0mg/ml condition, and the blood clotting titre was not less than 1: 256.
4 nitrocellulose filters
Purchase is from Millipore company, and is white, even, smooth, indefectible, and width is 20.0mm, Packing Sound.
The plain film of 5 glass fibre
Available from Nanjing glass fibre designing institute, white, indefectible, filament is evenly fine and closely woven.
6 absorbent filters
Quality is even, flat appearance, indefectible, white.
7 hard plastic boards
Smooth clean, distortionless distortion, gelatine layer does not evenly have accumulation.
Two, preparation method
1, the mark of mouse-anti AFP
Prepare colloidal gold solution earlier, get the 100ml purified water and place a clean container, on the magnetic force heating stirrer, be heated with stirring to boiling, the gold chloride that adds 5.0ml1.0%, be heated to boiling under stirring, the trisodium citrate that adds 6.0ml2.0% again, heated and stirred and seethed with excitement 10 minutes after, be cooled to room temperature and keep in Dark Place stand-by.Get the above-mentioned colloidal gold solution 100ml for preparing, regulate pH value to 8.0, slowly add 2.0mg mouse-anti AFP monoclonal antibody, stirred 30 minutes with sal tartari; The BSA2.0ml of adding 20% stirred 30 minutes; With 4 ℃ of high speed freezing centrifuges, centrifugal 30 minutes of 12000rpm, abandoning supernatant is precipitated to 100ml with the redissolution of 0.02M pH7.4 PBS solution.
2, the preparation of gold mark glass fibre membrane
According to 500ml/M
2Ratio with the composite solution of mouse-anti AFP monoclonal antibody gold label, be added on the glass fibre membrane, and dry more than 12 hours with vacuum freeze drier, obtain gold and mark glass fibre membrane.
3, the bag quilt of sheep anti-mouse igg antibody and agglutinin
To 1.5mg/ml, agglutinin is diluted to 1.2mg/ml with 0.02M pH7.4PBS with 0.02M pH7.4PBS dilution sheep anti-mouse igg antibody.Point of adjustment film machine (BIO DOT XYZ3000 of company or XY3000 type point film machine, concrete producer type does not have strict demand, can spray uniform straight line on film is standard) the some film location is respectively T line (the T line is a detection line) 25.7mm (illustrate: these data are the distance of spraying line position and plastic bottom board lower edge), C line (the C line is a control line) 30.2mm (illustrate: these data are the distance of spray line position and plastic bottom board lower edge), and nitrocellulose filter is attached on the hard plastic board, the distance of the lower edge of the paste position of nitrocellulose filter and the lower edge of plastic bottom board is 17.0mm.To dilute respectively in C pump that good sheep anti-mouse igg antibody, agglutinin be injected into a film machine, the T pump, according to 0.75 μ l/cm
2Amount be sprayed onto on the nitrocellulose filter.The nitrocellulose filter that spray is good is positioned over humidity and is not higher than 30%, and temperature was not higher than 25 ℃ drying room inner drying more than 12 hours.
4, assembling
Gold is marked glass fibre membrane, and to be cut into width be 7.0mm, and absorbent filter is cut into 24.0mm, inhales the sample glass fibre membrane and be cut into 15.0mm.Absorbent filter is pasted on the upper end of nitrocellulose filter (hard plastic board), nitrocellulose filter 1.0mm is pressed at the edge, gold mark glass fibre membrane is pasted on nitrocellulose filter (hard plastic board) lower end, nitrocellulose filter 1.0mm is pushed down at the edge, inhale the sample glass fibre membrane and be pasted on gold mark glass fibre membrane lower end, push down gold mark glass fibre membrane 2.0mm.After being assembled into, and by required width slitting (such as 4.0mm), in the plastic clip of packing into or be assembled into detector bar, by the specification packing.
Test example 1 agent plate of the present invention detects sensitivity, the specificity test of liver cancer
1, the sensitivity of agent plate of the present invention (embodiment 1 is prepared) detects
Collect 50 parts of cancer patient blood samples of Beijing You An hospital, blood sample background and testing result see Table 1.
Table 1
In this 50 increment basis, have 10 parts of primary carcinoma of liver HCC samples, the testing result of test strips of the present invention is all positive, and recall rate is 100.0%; 40 parts of other liver cancer samples detect 32 parts, and promptly the positive rate of reagent strip of the present invention is 32/40 * 100%=80.0%.
2 specific detection
Detect 50 parts of common pregnant woman's Cord blood samples, testing result sees Table 2.
Table 2
| Sample number | AFP content | Test strips testing result of the present invention |
| 1 | 850.00 | - |
| 2 | 679.50 | - |
| 3 | 647.90 | - |
| 4 | 458.10 | - |
| 5 | 29342.00 | - |
| 6 | 94380.00 | - |
| 7 | 18220.00 | - |
| 8 | 994.60 | - |
| 9 | 362.00 | - |
| 10 | 846.30 | - |
| 11 | 411.90 | - |
| 12 | 342.97 | - |
| 13 | 1673.37 | - |
| 14 | 2539.63 | - |
| 15 | 3380.15 | - |
| 16 | 439.83 | - |
| 17 | 690.55 | - |
| 18 | 13545.59 | - |
| 19 | 1948.91 | - |
| 20 | 41149.92 | - |
| 21 | 553.94 | - |
| 22 | 495.28 | - |
| 23 | 483.62 | - |
| 24 | 406.66 | - |
| 25 | 3254.61 | - |
| 26 | 5837.05 | - |
| 27 | 2564.65 | - |
| 28 | 599.21 | - |
| 29 | 361.50 | - |
| 30 | 552.73 | - |
| 31 | 385.61 | - ? |
| 32 | 338.29 | - |
| 33 | 637.70 | - |
| 34 | 753.51 | - |
| 35 | 844.81 | - |
| 36 | 373.68 | - |
| 37 | 447.57 | - |
| 38 | 1471.98 | - |
| 39 | 677.80 | - |
| 40 | 2295.78 | - |
| 41 | 1176.80 | - |
| 42 | 889.54 | - |
| 43 | 838.13 | - |
| 44 | 543.41 | - |
| 45 | 98466.84 | - |
| 46 | 54276.69 | - |
| 47 | 1432.15 | - |
| 48 | 404.87 | - |
| 49 | 1170.40 | - |
| 50 | 475.79 | - |
For the sample of non-AFP-L3, the recall rate of test strips of the present invention is 0, and promptly specificity reaches 100.0%.
3 sensitivity detect
This detection sample is to use the HCC serum sample to use negative serum dilution back to use the ELISA quantification kit to demarcate the standard items that obtain down concentration shown in the tabulation 3 by a certain percentage.
Table 3
| Through demarcate and dilution the HCC sample | Test strips testing result of the present invention |
| 400ng/ml | + |
| 200ng/ml | + |
| 100ng/ml | + |
| 50ng/ml | + |
| 25ng/ml | +- |
| 12.5ng/ml | - |
The detection lower limit of this detection is approximately 25ng/ml.
Claims (10)
1, a kind of agent plate that detects liver cancer is characterized in that: be made up of hard plate and the reagent strip that is attached on the hard plate; Described reagent strip is according to top-down order, successively by absorbent filter, nitrocellulose filter, be adsorbed with golden mark mouse-anti AFP monoclonal antibody glass fibre membrane and inhale sample glass fibre film close and be stitched together and form; Wherein, described nitrocellulose filter has a detection line that is formed by the agglutinin bag at the end near glass fibre membrane, at the other end near absorbent filter a control line that is formed by the sheep anti-mouse igg bag is arranged; There is row's well the lower end of described suction sample glass fibre membrane.
2, according to the agent plate of claim 1, it is characterized in that, the glass fibre membrane preparation method of the described mouse-anti AFP monoclonal antibody that is adsorbed with golden mark is as follows: will evenly be added on the glass fibre membrane behind the mouse-anti AFP monoclonal anti body and function colloidal gold solution mark, and freeze drying, promptly.
3, according to the agent plate of claim 1, it is characterized in that: described hard plate is selected from hard plastic board, hard plank or solid fiberboard.
4, according to the agent plate of claim 3, it is characterized in that: described hard plate is a hard plastic board.
5, a kind of method for preparing the described arbitrary agent plate of claim 1-4 comprises:
(1) preparation is adsorbed with the glass fibre membrane of the mouse-anti AFP monoclonal antibody of freeze-drying gold mark: will evenly be sprayed onto on the glass fibre membrane behind the mouse-anti AFP monoclonal anti body and function colloidal gold solution mark, and freeze drying, promptly;
(2) preparation contains the nitrocellulose filter of detection line and control line: after sheep anti-mouse igg antibody, agglutinin dilution, put respectively on 2 straight lines that parallel that nitrocellulose filter finishes in advance, and drying, promptly;
(3) nitrocellulose filter of absorbent filter with close detection line one end spliced mutually, the other end of nitrocellulose filter splices mutually with gold mark glass fibre membrane, and the other end of again gold being marked glass fibre membrane splices mutually with suction sample glass fibre membrane, obtains reagent strip; Fix or attach to reagent strip on the hard plate; Carrying out cutting or slitting according to needed specification, promptly.
6, according to the method for claim 5, it is characterized in that: in the step (1), the described cryodesiccated time is more than 12 hours.
7, according to the method for claim 5, it is characterized in that: in the step (2) with sheep anti-mouse igg antibody, agglutinin respectively according to 0.75 μ l/cm
2Amount put on 2 straight lines that parallel that nitrocellulose filter finishes in advance.
8, according to the method for claim 5, it is characterized in that: be more than 12 hours the drying time described in the step (2).
9, according to the method for claim 5, it is characterized in that: in the step (3), at first gold being marked glass fibre membrane, to be cut into width be 7.0mm, and it is 24.0mm that absorbent filter is cut into width, and inhaling the sample glass fibre membrane, to be cut into width be 15.0mm; The nitrocellulose filter of absorbent filter with close detection line one end spliced mutually, and wherein, nitrocellulose filter 1.0mm is pressed at the absorbent filter edge; The other end of nitrocellulose filter splices mutually with gold mark glass fibre membrane, and wherein nitrocellulose filter 1.0mm is pushed down at the edge of gold mark glass fibre membrane; Inhale the sample glass fibre membrane and be pasted on gold mark glass fibre membrane lower end, push down gold mark glass fibre membrane 2.0mm.
10, the purposes of arbitrary agent plate of claim 1-4 in preparation detection or diagnosing liver cancer reagent.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101765922A CN101424690A (en) | 2007-10-31 | 2007-10-31 | Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications |
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|---|---|---|---|
| CNA2007101765922A CN101424690A (en) | 2007-10-31 | 2007-10-31 | Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102062777A (en) * | 2009-11-12 | 2011-05-18 | 上海科新生物技术股份有限公司 | Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof |
| CN102621314A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-Cenp-B antibody detection test paper and preparation method thereof |
| CN102621311A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-SSB antibody detection test paper and preparation method thereof |
| CN102625915A (en) * | 2009-07-14 | 2012-08-01 | 独立行政法人产业技术综合研究所 | Method for measurement of glycoprotein, method for detection of hepatic diseases, reagent for quantification of glycoprotein, and sugar chain marker glycoprotein as measure of disease conditions of hepatic diseases |
| CN102621313A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-Sp100 antibody detection test paper and preparation method thereof |
| CN103822878A (en) * | 2012-11-16 | 2014-05-28 | 上海市肿瘤研究所 | Lectin functionalized nanogold, and preparation method and application thereof |
| CN111363037A (en) * | 2020-05-26 | 2020-07-03 | 北京瀚梅生物科技有限公司 | Disease detection kit containing antibody specifically binding AFP protein |
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- 2007-10-31 CN CNA2007101765922A patent/CN101424690A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102625915A (en) * | 2009-07-14 | 2012-08-01 | 独立行政法人产业技术综合研究所 | Method for measurement of glycoprotein, method for detection of hepatic diseases, reagent for quantification of glycoprotein, and sugar chain marker glycoprotein as measure of disease conditions of hepatic diseases |
| CN102062777A (en) * | 2009-11-12 | 2011-05-18 | 上海科新生物技术股份有限公司 | Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof |
| CN102062777B (en) * | 2009-11-12 | 2013-08-28 | 上海科新生物技术股份有限公司 | Hepatopathy detecting test paper by colloidal gold chromatography and preparation method thereof |
| CN102621314A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-Cenp-B antibody detection test paper and preparation method thereof |
| CN102621311A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-SSB antibody detection test paper and preparation method thereof |
| CN102621313A (en) * | 2011-01-28 | 2012-08-01 | 上海科新生物技术股份有限公司 | Colloidal gold chromatography anti-Sp100 antibody detection test paper and preparation method thereof |
| CN103822878A (en) * | 2012-11-16 | 2014-05-28 | 上海市肿瘤研究所 | Lectin functionalized nanogold, and preparation method and application thereof |
| CN111363037A (en) * | 2020-05-26 | 2020-07-03 | 北京瀚梅生物科技有限公司 | Disease detection kit containing antibody specifically binding AFP protein |
| CN111363037B (en) * | 2020-05-26 | 2020-11-17 | 深圳迈吉赛尔生物科技有限公司 | Disease detection kit containing antibody specifically binding AFP protein |
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Application publication date: 20090506 |