CN101422150A - New use of prodigiosin - Google Patents
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- CN101422150A CN101422150A CNA2007100476183A CN200710047618A CN101422150A CN 101422150 A CN101422150 A CN 101422150A CN A2007100476183 A CNA2007100476183 A CN A2007100476183A CN 200710047618 A CN200710047618 A CN 200710047618A CN 101422150 A CN101422150 A CN 101422150A
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Abstract
The invention relates to applications of using prodigiosin produced by fermentation of Serratieae as an alga killing agent and/or an alga inhibitor; the alga killing agent and/or the alga inhibitor can kill and/or inhibit chlorella, Karenia mikimotoi, thin Chaetoceros gracilis, Chaetoceros curvisetus, Skeletonema costatus, Nitzschia closterium and Dunaliella and the like which can cause red tide as well as Anabaena, Microcystic aeruginosa, tiny Merismopedia and blue algae and the like which can cause algae bloom.
Description
Technical field
The present invention relates to be derived from the prodigiosin that Serratieae (Serratia) microorganism belonging to genus is produced, this haematochrome has except that algae algal control effect, especially utilize contain prodigiosin as active component remove algae algal control preparation, and utilize above-mentioned bacterial strains fermentation culture or prodigiosin to remove the method for red tide and wawter bloom algae.
Background technology
Along with the World Economics fast development, the mankind are damaging the earth unawares in various activities, and worldwide environmental pollution is threatening human safety.Human solving on the problem of environmental pollution, experienced four courses such as industrial pollution improvement, city environmental pollution integrated control, ecotope integrated control, regional pollution control.Since the fifties in last century, people recognize that gradually threatening the environmental problem of human survival is global problem, and global enhancement of environment will lean on the environmental improvement in each some areas or zone to be the basis.
Red tide pollutes as a kind of global water, be meant that fulminant under the certain environment condition such as some plankton in the ocean (referring to algae especially), protozoa or bacterium is bred or gathering reaches certain level, cause that water colour changes or other marine organisms are produced a kind of ecological anomaly of damaging effect, be also referred to as harmful algal bloom (harmful algae bloom, HAB).
" wawter bloom " (water blooms) is a kind of natural, ecological phenomenon in the fresh water, is only to be caused by algae, as blue-green algae (should be called cyanobacteria on the stricti jurise), green alga, diatom etc., the just eutrophication of water.When " wawter bloom " took place, one was blue or green water.This " wawter bloom " phenomenon that just has at natural world, just on the books on Ancient Times in China history.In their very fast disappearances of occurring in nature, do not bring harm to aquatic products animal and human class.
Red tide and wawter bloom have been destroyed water ecosystem, have endangered water environment, aquatile and human health directly or indirectly, and to fresh water mariculture industry, fishery, tourism and leisure industry constitute a serious threat, and are one of numerous substantial pollution problems in the world.
According to Chinese Oceanic disasters communique in 2005, red tide was found 82 times altogether in China marine site in 2005, wherein the Bohai Sea is 9 times, the Huanghai Sea 13 times, the East Sea 51 times, the South Sea 9 times, than reducing about 15% last year, about 27070 square kilometres of area takes place in accumulative total, remain basically stable with last year, but red tide number of times and area that poisonous algae causes significantly increases.2001-2005 years, red tide took place 453 times, 93 260 square kilometres of cumulative area in full marine site altogether.The red tide of area above 100 square kilometres takes place 132 times in full marine site altogether, about 86 520 square kilometres of cumulative area.Red tide has significant temporal characteristics.The monitoring result demonstration, the multiple phase of red tide concentrates on for 5-June, and red tide accumulative total frequency and accumulative total generating plane integration do not account for 62% and 83% of the whole year.
The frequency that the China recent years wawter bloom takes place is also more and more higher, and it is also more and more to relate to the area.
Calendar year 2001, the wawter bloom phenomenon has appearred in river Lake Water Body such as the long river of Beijing City, Kun Yuhe, the North Sea, Zhongnanhai.
In mid-February, 2003, the red wawter bloom of iron rust takes place in Yunnan Man Wan reservoir in the scope of 30km reservoir area, dam, and wawter bloom is ribbon, through being accredited as the dinoflagellate wawter bloom, nearly one month of this wawter bloom duration.
In addition, it is comparatively rare the wawter bloom phenomenon to occur as the Han River bigger floating-channel of this class.Big wawter bloom has taken place in the period of 11 5 times unexpectedly.Just be not seen in report in the past in 1991, Han River wawter bloom phenomenon in 2003 is the 4th time that river, Wuhan, Han River section took place after 1992,1998,2000.
In March, 2006, the algae in your river, general main canal drinking water seedbed of Xuchang City Ying mainly is a diatom, and particularly the quantity of straight chain diatom is bigger, become sociales, with a spot of ring algae, the number of diatom reaches 3123 individuality/milliliters, and forms the water surface visible " wawter bloom ".
China lake has faced eutrophication problem, and eutrophication has become the of paramount importance environmental problem of China's water environment.Along with increasingly sharpening of environmental pollution, the blue-green algae problem in the bridge reservoir of large-scale fresh water lake such as the Taihu Lake of China, Chaohu, Dian Chi, Wuhan East Lake and Dahuofang Reservoir, Fushun, Diversion Project from Luanhe to Tianjin is also very serious, and presents development trend.
(Prodigiosins is the general name of a natural red colouring matter family PG) to prodigiosin, is by multiple actinomycetes (Streptomyces) and bacterium (Serratia, Pseudomonas) a class secondary metabolite of Chan Shenging.The methoxyl group pyrrole skeleton structure that prodigiosin all is made up of three pyrrole rings usually.
Prodigiosin has biologic activity widely, comprise anticancer cytoactive, antibacterium (antibacterial), anti-malarial (antimalarial), antimycotic (antifungal), antiprotozoals (antiprotozoal), antiulcer (1997) and treatment mouse leukemia (1986), the mouse diabetes (2001) of extremely strong immunosuppressive activity, high-efficiency low-toxicity, and can be used as vegetable insecticide (2000) etc.
But the algae algal control effect that removes of prodigiosin is still not fully aware of.
Summary of the invention
The purpose of this invention is to provide purposes and the using method thereof of prodigiosin in removing the algae algal control.
In a first aspect of the present invention, provide a kind of application with haematochrome of three pyrrole rings composition methoxyl group pyrrole skeleton structures as algae agent for killing and/or alga inhibitor.
In another preference, described haematochrome is suc as formula the prodigiosin shown in the I:
Formula I
In another preference, described algae comprises the algae that causes red tide and/or wawter bloom.
In another preference, describedly cause that the algae of red tide is selected from chlorella (Chlorella spp.), the triumphant human relations algae of Michaelis (Gymnodinium mikimotoi), very thin Chaetoceros (Chaetoceros gracilis), revolves chain Chaetoceros (Chaetoceros curvisetus), middle Skeletonemacostatum (Skeletonema costatum), Nitzschia closterium minutissima (Nitzschia closterium), Dunaliella salina (Dunaliella saline), Lan Yinzao (Chroomonas placoidea).
In another preference, the described algae that causes wawter bloom is selected from wawter bloom anabena (Anabenaflos_aquae), microcystic aeruginosa (Microcystis aeruginosa), smallly places side by side algae (Merismopedia sp.), blue-green algae (Cyanobactria).
In another preference, described algae agent for killing and/or alga inhibitor comprise liquid formulation and solid formulation.
In another preference, having at least a kind of in the active component in described liquid preparation or the solid formulation is suc as formula the prodigiosin shown in the I.
In a second aspect of the present invention, provide a kind of use to have three pyrrole rings and formed the method that algae was killed and/or suppressed to the haematochrome of methoxyl group pyrrole skeleton structure, described method comprises step: the aqueous systems of described haematochrome and red tide and/or wawter bloom is mixed, and the mixed proportion of wherein said haematochrome and algae is 〉=0.01 μ g haematochrome: 1.0 * 10
5Individual alga cells, preferably, mixed proportion is 0.01-200 μ g haematochrome: 1.0 * 10
5Individual alga cells, described algae preferably is selected from the algae that causes red tide and/or wawter bloom;
Describedly cause that the algae of red tide is selected from chlorella (Chlorella spp.), the triumphant human relations algae of Michaelis (Gymnodinium mikimotoi), very thin Chaetoceros (Chaetoceros gracilis), revolves chain Chaetoceros (Chaetoceros curvisetus), middle Skeletonemacostatum (Skeletonema costatum), Nitzschia closterium minutissima (Nitzschia closterium), Dunaliella salina (Dunaliella saline), Lan Yinzao (Chroomonas placoidea);
The described algae that causes wawter bloom is selected from wawter bloom anabena (Anabena flos_aquae), microcystic aeruginosa (Microcystis aeruginosa), smallly places side by side algae (Merismopedia sp.), blue-green algae (Cyanobactria).
In another preference, the mixed proportion of described haematochrome and algae is 〉=0.05 μ g haematochrome: 1.0 * 10
5Individual alga cells, more preferably, mixed proportion is 0.05-25 μ g haematochrome: 1.0 * 10
5Individual alga cells.
In another preference, in the aqueous systems of red tide and/or wawter bloom, the working concentration of haematochrome 〉=0.01 μ g/ml, more preferably, the working concentration of haematochrome is 0.01-200 μ g/ml; Described haematochrome is preferably suc as formula the prodigiosin shown in the I.
In another preference, in the aqueous systems of red tide and/or wawter bloom, the working concentration of haematochrome 〉=0.05 μ g/ml, more preferably, the working concentration of haematochrome is 0.05-25 μ g/ml.
In another preference, described haematochrome is preferably suc as formula the prodigiosin shown in the I.
In view of the above, the invention provides new purposes and the using method thereof of prodigiosin in removing the algae algal control.
Description of drawings
Fig. 1 demonstrates the remove algae effect of prodigiosin to chlorella (Chlorella spp.).
Fig. 2 demonstrates the algal control effect of prodigiosin to chlorella (Chlorella spp.).
Fig. 3 demonstrates the remove algae effect of prodigiosin to the triumphant human relations algae of Michaelis (Gymnodinium mikimotoi).
Fig. 4 demonstrates the remove algae effect of prodigiosin to very thin Chaetoceros (Chaetoceros gracilis).
Fig. 5 demonstrates the algal control effect of prodigiosin to very thin Chaetoceros (Chaetoceros gracilis).
Fig. 6 demonstrates prodigiosin to revolving the algae effect that removes of chain Chaetoceros (Chaetoceros curvisetus).
Fig. 7 demonstrates the algae effect that removes of prodigiosin centering Skeletonemacostatum (Skeletonema costatum).
Fig. 8 demonstrates the algal control effect of prodigiosin centering Skeletonemacostatum (Skeletonema costatum).
Fig. 9 demonstrates the remove algae effect of prodigiosin to Nitzschia closterium minutissima (Nitzschia closterium).
Figure 10 demonstrates the algal control effect of prodigiosin to Nitzschia closterium minutissima (Nitzschia closterium).
Figure 11 demonstrates the remove algae effect of prodigiosin to Lan Yinzao (Chroomonas placoidea).
Figure 12 demonstrates the remove algae effect of prodigiosin to wawter bloom anabena (Anabena flos_aquae).
Figure 13 demonstrates the algal control effect of prodigiosin to wawter bloom anabena (Anabena flos_aquae).
Figure 14 demonstrates the algal control effect of prodigiosin to microcystic aeruginosa (Microcystis aeruginosa).
Figure 15 demonstrates prodigiosin to the small algae effect that removes of placing side by side algae (Merismopedia sp.).
Figure 16 demonstrates the remove algae effect of prodigiosin to blue-green algae (Cyanobactria).
The prodigiosin crude product that Figure 17 demonstrates extraction causes the triumphant human relations algae of Michaelis (Gymnodinium mikimotoi) metamorphosis owing to removing algae algal control effect.
The prodigiosin crude product that Figure 18 demonstrates extraction causes middle Skeletonemacostatum (Skeletonema costatum) metamorphosis owing to removing algae algal control effect.
The prodigiosin crude product that Figure 19 demonstrates extraction causes wawter bloom anabena (Anabena flos_aquae) metamorphosis owing to removing algae algal control effect.
The prodigiosin crude product that Figure 20 demonstrates extraction causes small algae (Merismopedia sp.) metamorphosis of placing side by side owing to removing algae algal control effect.
The prodigiosin crude product that Figure 21 demonstrates extraction causes blue-green algae (Cyanobactria) metamorphosis owing to removing algae algal control effect.
Embodiment
The inventor finds the effect that the algae that causes red tide and wawter bloom is had killing and/or suppresses suc as formula the prodigiosin shown in the I through extensive and deep research.
As used herein, " remove algae " just is meant that haematochrome provided by the invention kills the function of algae activity, can represent with removing the active % of algae:
Remove in the active %=[(control sample of algae uncracked cell density in uncracked cell density-sample)/control sample in uncracked cell density] * 100%.
As used herein, " algal control " just is meant that growth produces inhibitory action to haematochrome provided by the invention to alga cells, thereby avoids the quick breeding of algae.
The above-mentioned feature that the present invention mentions, or the feature that embodiment mentions can make up arbitrarily.All features that this case specification is disclosed can with any composition forms and usefulness, each feature that is disclosed in the specification can anyly provide the alternative characteristics of identical, impartial or similar purpose to replace.Therefore removing has special instruction, and the feature that is disclosed only is the general example of equalization or similar features.
Major advantage of the present invention is:
1, prodigiosin can kill the alga cells that causes red tide and wawter bloom at short notice under very low working concentration or suppress algae grows, is a kind of efficient algae algae-inhibiting agent that removes.
2, prodigiosin is seen photolysis to photaesthesia, can not bring secondary pollution to water body.
Below by particular specific embodiment embodiments of the present invention are described, those skilled in the art can understand other advantage of the present invention and effect easily by the content that embodiment disclosed.The present invention also can be implemented or be used by other different specific embodiment, and the every details in this specification also can be based on different viewpoints and application, carries out various modifications and change under the spirit of the present invention not deviating from.
Embodiment 1
The separation of prodigiosin and extraction
The bacterial strain that this embodiment adopts is serratia marcescens (Serratia marcescens), available from The 2nd Army Medical College.
(table 1) inoculates this bacterial strain on solid culture medium, cultivates 48 hours for 30 ℃, and fresh culture is changed in preservation in 4 ℃ of refrigerators weekly then, the cultivation of going down to posterity.
Table 1. solid culture medium
| Composition | Concentration |
| Glucose | 20g/L |
| Dusty yeast | 10g/L |
| Peptone | 10g/L |
| Agar powder | 20g/L |
| pH | 7.2 |
With the colony inoculation on the solid culture medium to liquid seed culture medium (table 2), 28 ℃ and 200rpm cultivation 12h, be seeded to then in the fermentation medium (table 3), fermentation process later stage flow feeding medium (table 4), 25 ℃ and dissolved oxygen〉20% cultivation 36h, isolate prodigiosin at last from fermentation culture.
Table 2. seed culture medium
| Composition | Concentration |
| Dusty yeast | 1g/L |
| Peptone | 2g/L |
| Glycerine | 12.6g/L |
| (NH 4) 2SO 4 | 6g/L |
| K 2HPO 4 | 10g/L |
| NaCl | 0.5g/L |
| MgSO 4 | 0.5g/L |
| pH | 7.2 |
Table 3. fermentation medium
| Composition | Concentration |
| Peptone | 30g/L |
| Glycerine | 12.6g/L |
| Zn 2SO 4 | 0.2g/L |
| K 2HPO 4 | 3g/L |
| NaCl | 0.5g/L |
| MnSO 4 | 0.05g/L |
| pH | 7.2 |
Table 4. supplemented medium
| Composition | Concentration |
| Glycerine | 5g/L |
| Peptone | 30g/L |
Separation and Extraction prodigiosin then, employed separating and extracting process is 200410017773.7 Chinese patent application referring to application number.
The algae algal control effect that removes of prodigiosin
Algae and source that present embodiment adopted are shown in Table 5.
Table 5 experiment algae and source
Adopt f/2 medium culture marine microalgae shown in the table 6, adopt BG1 medium culture freshwater microalgae shown in the table 7, in illumination box, cultivate under 23 ℃ of 3000Lux light intensity, the circulation of 12h/12h light and shade.Adopt the frustule of exponential phase to detect of the remove algae algal control effect of prodigiosin crude product to various algae.Adopt light microscope to detect the algal grown situation, or be fixed in the 2%Rugol solution, on blood counting chamber, count.
Table 6 f/2 medium
| Composition | Concentration |
| NaNO 3 | 74.8mg |
| NaH 2PO 4 | 4.4mg |
| Na 2SiO 3.9H 2O | 8.4—16.7mg |
| The f/2 trace element solution | 1ml |
| The f/2 vitamin solution | 1ml |
| Artificial seawater | 1000ml |
The f/2 trace element solution
| Composition | Concentration |
| ZnSO 4.4H 2O | 23mg |
| MnCl 2.4H 2O | 78mg |
| CuSO 4.5H 2O | 10mg |
| FeC 6H 5O 7.5H 2O | 3.9mg |
| Na 2MoO 42H 2O | 7.3mg |
| CoCl 2.6H 2O | 12mg |
| Na 2EDTA | 4.35mg |
| Pure water | 1000ml |
The f/2 vitamin solution
| Composition | Concentration |
| Cobastab 12 | 0.5mg |
| Cobastab 1 | 100mg |
| Biotin | 0.5mg |
| Pure water | 1000ml |
Table 7 BG1 medium
| Composition | Concentration |
| NaNO 3 | 1.5g |
| K 2HPO 4.3H 2O | 0.04g |
| MgSO 4.7H 2O | 0.075g |
| CaCl 2.2H 2O | 0.036g |
| Citric acid | 0.006g |
| Ferric citrate (Ferric ammonium citrate) | 0.006g |
| EDTA(dinatrium-salt) | 0.001g |
| Na 2CO 3 | 0.02g |
| A5+Co solution | 1ml |
| Distilled water | 1000ml |
A5+Co solution
| Composition | Concentration |
| H 3BO 3 | 2.86g |
| MnCl 2.H 2O | 1.81g |
| ZnSO 4.7H 2O | 0.222g |
| CuSO 4.5H 2O | 0.079g |
| Na 2MoO 4.2H 2O | 0.390g |
| Co(NO 3) 2.6H 2O | 0.049g |
| Distilled water | 1000ml |
Certain density prodigiosin acidic methanol concentrate is diluted to desired concn with pure water, add 0.6ml prodigiosin dilution to the algae liquid 29.4ml that reaches exponential phase, shake up, microscopically is counted after continuing the illumination cultivation certain hour, only the complete frustule of cracking does not take place in counting, determines algicdal activity.
The algicdal activity computational methods:
Remove in the active %=[(control sample of algae uncracked cell density in uncracked cell density one sample)/control sample in uncracked cell density] * 100%
(1) prodigiosin is to the algae effect that removes of chlorella (Chlorella spp.).
Chlorella initial density: 1.0 * 105cells/ml, after prodigiosin adds the algae mixed system, its activity: 0.1 μ g/ml, 1.0 μ g/ml and 6.0 μ g/ml.As seen from Figure 1,1 μ g/ml prodigiosin removes the algae activity near 100 during 1h.
(2) prodigiosin is to the algal control effect of chlorella (Chlorella spp.)
Chlorella initial density: 1.0 * 105cells/ml, the prodigiosin activity: 0.1 μ g/ml, 0.5 μ g/ml and 1 μ g/ml. as seen from Figure 2,0.1 μ g/ml prodigiosin can well suppress the growth of chlorella.
(3) prodigiosin is to the algae effect that removes of the triumphant human relations algae of Michaelis (Gymnodinium mikimotoi).
The triumphant human relations algae of Michaelis initial density: 2.0 * 104cells/ml, prodigiosin activity: 25 μ g/ml, 15 μ g/ml and 5.0 μ g/ml.As seen from Figure 3,25 μ g/ml prodigiosins remove the algae activity and surpass 80 during 48h.Figure 17 is seen in the triumphant human relations frustule contrast of the Michaelis that form changes after normal triumphant human relations frustule of Michaelis and the prodigiosin effect.
(4) prodigiosin is to the algae effect that removes of very thin Chaetoceros (Chaetoceros gracilis).
Very thin Chaetoceros initial density: 3.0 * 105cells/ml, prodigiosin activity: 1.0 μ g/ml, 3.5 μ g/ml and 7.0 μ g/ml.As seen from Figure 4,9h, 3.5 μ g/ml prodigiosins remove the algae activity and reach 100.
(5) prodigiosin is to the algal control effect of very thin Chaetoceros (Chaetoceros gracilis).
Very thin Chaetoceros initial density: 1.0 * 105cells/ml, prodigiosin activity: 0.1 μ g/ml.0.05 μ g/ml. as seen from Figure 5,0.05 μ g/ml prodigiosin promptly can suppress the growth of very thin Chaetoceros.
(6) prodigiosin is to revolving the algae effect that removes of chain Chaetoceros (Chaetoceros curvisetus).
Revolve chain Chaetoceros initial density: 3.0 * 105cells/ml, the prodigiosin activity: 1.0 μ g/ml, 3.5 μ g/ml and 7.0 μ g/ml.As seen from Figure 6,9h, 1.0 μ g/ml prodigiosins remove the algae activity and reach 80.
(7) the algae effect that removes of prodigiosin centering Skeletonemacostatum (Skeletonema costatum).
Middle Skeletonemacostatum initial density: 1.0 * 105cells/ml, prodigiosin activity: 1.0 μ g/ml and 7.0 μ g/ml.As seen from Figure 7,1h, 7 μ g/ml prodigiosins remove the algae activity near 100.Figure 18 is seen in the middle Skeletonemacostatum cell contrast that form changes after Skeletonemacostatum cell and the prodigiosin effect in normal.
(8) the algal control effect of prodigiosin centering Skeletonemacostatum
Middle Skeletonemacostatum initial density: 1.0 * 105cells/ml, prodigiosin activity: 0.5 μ g/ml, 0.1 μ g/ml and 0.05 μ g/ml.As seen from Figure 8, the growth of Skeletonemacostatum during 0.05 μ g/ml prodigiosin can suppress fully.
(9) prodigiosin is to the algae effect that removes of Nitzschia closterium minutissima (Nitzschia closterium).
Nitzschia closterium minutissima initial density: 1.0 * 105cells/ml, prodigiosin activity: 10.0 μ g/ml, 5.0 μ g/ml and 1.0 μ g/ml.As seen from Figure 9,12h, 10 μ g/ml prodigiosins remove the algae activity and have reached 100.
(10) prodigiosin is to the algal control effect of Nitzschia closterium minutissima (Nitzschia closterium).
Nitzschia closterium minutissima initial density: 1.0 * 105cells/ml, prodigiosin activity: 0.1 μ g/ml, 0.5 μ g/ml and 1.0 μ g/ml.As seen from Figure 10,0.1 μ g/ml prodigiosin can suppress the growth of Nitzschia closterium minutissima fully.
(11) prodigiosin is to the algae effect that removes of salt algae (Dunaliella saline).
24h, prodigiosin is 0.18 μ g/ml to the LD50 of salt algae.
(12) prodigiosin is to the algae effect that removes of Lan Yinzao (Chroomonas placoidea).
Blue latent algae initial density: 1.0 * 105cells/ml, prodigiosin activity: 10.0 μ g/ml, 5.0 μ g/ml and 1.0 μ g/ml.As seen from Figure 11,5h, 1.0 μ g/ml prodigiosins remove the algae activity and have reached 100.
(13) prodigiosin is to the algae effect that removes of wawter bloom anabena (Anabena flos_aquae).
Wawter bloom anabena initial density: 1.0 * 105cells/ml, prodigiosin activity: 5.0 μ g/ml and 1.5 μ g/ml.As seen from Figure 12,12h, 5.0 μ g/ml prodigiosins remove the algae activity and have reached 100.Figure 19 is seen in the wawter bloom anabena cell contrast that form changes after normal wawter bloom anabena cell and the prodigiosin effect.
(14) prodigiosin is to the algal control effect of wawter bloom anabena (Anabena flos_aquae).
Wawter bloom anabena initial density: 1.0 * 105cells/ml, prodigiosin activity: 1.0 μ g/ml, 0.5 μ g/ml and 0.1 μ g/ml.As seen from Figure 13,0.1 μ g/ml prodigiosin promptly can suppress the growth of wawter bloom anabena.
(15) prodigiosin is to the algae effect that removes of microcystic aeruginosa (Microcystis aeruginosa).
Microcystic aeruginosa initial density: 2.4 * 105cells/ml, 24h, prodigiosin is 20.1 μ g/ml to the LD50 of microcystic aeruginosa
(16) prodigiosin is to the algal control effect of microcystic aeruginosa (Microcystis aeruginosa).
Microcystic aeruginosa initial density: 1.0 * 105cells/ml, the prodigiosin activity: 5.0 μ g/ml, 1.0 μ g/ml and 0.5 μ g/ml. as seen from Figure 14,1.0 μ g/ml prodigiosins promptly can suppress the growth of microcystic aeruginosa.
(17) prodigiosin is to the small algae effect that removes of placing side by side algae (Merismopedia sp.).
Small algae initial density: the 1.0 * 105cells/ml that places side by side, prodigiosin activity: 5.0 μ g/ml and 1.5 μ g/ml.As seen from Figure 15,12h, 1.5 μ g/ml prodigiosins remove the algae activity and have reached 80.The normal small small frustule of placing side by side of placing side by side that form changes after frustule and the prodigiosin effect contrasts and sees Figure 20.
(18) prodigiosin removes the algae action effect to Taihu Lake blue-green algae (Cyanobactria).
Blue-green algae initial density: 1.0 * 105cells/ml, prodigiosin activity: 5.0 μ g/ml, 1.0 μ g/ml and 0.5 μ g/ml.As seen from Figure 16,24h, 5.0 μ g/ml prodigiosins remove the algae activity and have reached 80.Figure 21 is seen in the blue-green algae cell contrast that form changes after normal blue-green algae cell and the prodigiosin effect.
The above only is preferred embodiment of the present invention, be not in order to limit essence technology contents scope of the present invention, essence technology contents of the present invention is broadly to be defined in the claim scope of application, any technology entity or method that other people finish, if it is defined identical with the claim scope of application, also or a kind of change of equivalence, all will be regarded as being covered by among this claim scope.
Claims (10)
1. application with haematochrome of three pyrrole rings composition methoxyl group pyrrole skeleton structures as algae agent for killing and/or alga inhibitor.
2. application as claimed in claim 1 is characterized in that, described haematochrome is suc as formula the prodigiosin shown in the I:
Formula I
3. as claim 1 or 2 arbitrary described application, it is characterized in that described algae comprises the algae that causes red tide and/or wawter bloom.
4. application as claimed in claim 3 is characterized in that, describedly causes that the algae of red tide is selected from chlorella, the triumphant human relations algae of Michaelis, very thin Chaetoceros, revolves chain Chaetoceros, middle Skeletonemacostatum, Nitzschia closterium minutissima, Dunaliella salina, Lan Yinzao.
5. application as claimed in claim 3 is characterized in that, the described algae that causes wawter bloom is selected from wawter bloom anabena, microcystic aeruginosa, smallly places side by side algae, blue-green algae.
6. application as claimed in claim 1 is characterized in that, described algae agent for killing and/or alga inhibitor comprise liquid formulation and solid formulation.
7. a use has three pyrrole rings and forms the method that algae was killed and/or suppressed to the haematochrome of methoxyl group pyrrole skeleton structures, it is characterized in that, described method comprises step: the aqueous systems of described haematochrome and red tide and/or wawter bloom is mixed, and the mixed proportion of wherein said haematochrome and algae is 〉=0.01 μ g haematochrome: 1.0 * 10
5Individual alga cells, described algae preferably is selected from the algae that causes red tide and/or wawter bloom;
Describedly cause that the algae of red tide is selected from chlorella, the triumphant human relations algae of Michaelis, very thin Chaetoceros, revolves chain Chaetoceros, middle Skeletonemacostatum, Nitzschia closterium minutissima, Dunaliella salina, Lan Yinzao;
The described algae that causes wawter bloom is selected from wawter bloom anabena, microcystic aeruginosa, smallly places side by side algae, blue-green algae;
Described haematochrome is preferably suc as formula the prodigiosin shown in the I.
8. using method as claimed in claim 7 is characterized in that, mixed proportion is 〉=0.05 μ g haematochrome: 1.0 * 10
5Individual alga cells.
9. using method as claimed in claim 7 is characterized in that, in the aqueous systems of red tide and/or wawter bloom, and the working concentration of haematochrome 〉=0.01 μ g/ml; Described haematochrome is preferably suc as formula the prodigiosin shown in the I.
10. using method as claimed in claim 9 is characterized in that, the working concentration of haematochrome 〉=0.05 μ g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100476183A CN101422150A (en) | 2007-10-31 | 2007-10-31 | New use of prodigiosin |
Applications Claiming Priority (1)
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| CNA2007100476183A CN101422150A (en) | 2007-10-31 | 2007-10-31 | New use of prodigiosin |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387529A (en) * | 2013-07-30 | 2013-11-13 | 中国农业科学院烟草研究所 | Anti-TMV (Tobacco Mosaic Virus) tripyrrole ring compound and preparation method and application thereof |
| CN104844507A (en) * | 2015-04-01 | 2015-08-19 | 华中师范大学 | Application of quinolinone compounds to inhibit growth of alga and algicide for inhibiting growth of alga |
| CN108720032A (en) * | 2017-04-19 | 2018-11-02 | 嘉兴学院 | A kind of natural pigment pure cellulose food and preparation method thereof |
| CN111154673A (en) * | 2020-01-08 | 2020-05-15 | 江苏师范大学 | Prodigiosin producing strain and production method and application thereof |
| CN113973799A (en) * | 2021-10-12 | 2022-01-28 | 广东海洋大学 | Application of hydroxyethyl sulfonic acid in removing adsorption type benthic diatom |
| CN113994946A (en) * | 2021-10-12 | 2022-02-01 | 广东海洋大学 | Application of o-hydroxy cinnamic acid in removing harmful benthic diatom |
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2007
- 2007-10-31 CN CNA2007100476183A patent/CN101422150A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387529A (en) * | 2013-07-30 | 2013-11-13 | 中国农业科学院烟草研究所 | Anti-TMV (Tobacco Mosaic Virus) tripyrrole ring compound and preparation method and application thereof |
| CN103387529B (en) * | 2013-07-30 | 2016-03-30 | 中国农业科学院烟草研究所 | Tripyrrole cyclics of anti-TMV and its production and use |
| CN104844507A (en) * | 2015-04-01 | 2015-08-19 | 华中师范大学 | Application of quinolinone compounds to inhibit growth of alga and algicide for inhibiting growth of alga |
| CN104844507B (en) * | 2015-04-01 | 2017-12-26 | 华中师范大学 | Application of the quinolinones compound in algal grown is suppressed and the algicide for suppressing algal grown |
| CN108720032A (en) * | 2017-04-19 | 2018-11-02 | 嘉兴学院 | A kind of natural pigment pure cellulose food and preparation method thereof |
| CN111154673A (en) * | 2020-01-08 | 2020-05-15 | 江苏师范大学 | Prodigiosin producing strain and production method and application thereof |
| CN113973799A (en) * | 2021-10-12 | 2022-01-28 | 广东海洋大学 | Application of hydroxyethyl sulfonic acid in removing adsorption type benthic diatom |
| CN113994946A (en) * | 2021-10-12 | 2022-02-01 | 广东海洋大学 | Application of o-hydroxy cinnamic acid in removing harmful benthic diatom |
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