CN101417044A - Preparation method Kwangtung purple beautyberry extract and new use thereof - Google Patents
Preparation method Kwangtung purple beautyberry extract and new use thereof Download PDFInfo
- Publication number
- CN101417044A CN101417044A CNA2008101364048A CN200810136404A CN101417044A CN 101417044 A CN101417044 A CN 101417044A CN A2008101364048 A CNA2008101364048 A CN A2008101364048A CN 200810136404 A CN200810136404 A CN 200810136404A CN 101417044 A CN101417044 A CN 101417044A
- Authority
- CN
- China
- Prior art keywords
- preparation
- extract
- callicarpa kwangtungensis
- kwangtungensis chun
- column chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a Guangdong beautyberry extract and a new application thereof. The preparation method of the extract comprises the steps: the entire plants of Guangdong beautyberry, namely, roots, stems, leaves or fruits, are taken as raw materials, processed by one or a plurality of the solvent distilling method, the solvent extraction method, the resin adsorption method, the polyamide adsorption column chromatography, the normal-phase column chromatography and the reversed-phase column chromatography, and finally dried to prepare the Guangdong beautyberry extract. The preparation method also comprises the steps that the obtained Guangdong beautyberry extract is combined with an excipient by a conventional method and prepared into dosage forms, such as power, particles, tablets, capsules, pills, suppository, enteric-coated tablets, injections, syrup, emulsion, suspension, tincture, ointment or nebula. When the Guangdong beautyberry extract is prepared into different dosage forms, one or a plurality of a lubricant, the excipient or a bonding agent are added into the extract. The invention also provides an application of the Guangdong beautyberry extract to hemostasia. The techniques used by the invention are simple and easy. The extract has good curative effect on hemostasia treatment but no toxic and side effects.
Description
Technical field
The present invention relates to a kind of preparation method of Chinese medicine extract, relate in particular to a kind of preparation method of Callicarpa kwangtungensis Chun extract, also relate to the new purposes of this extract simultaneously.
Background technology
Callicarpa kwangtungensis Chun is a conventional Chinese medicine, has another name called hemostasis bavin, Folium Callicarpae Formosanae, Folium Callicarpae Formosanae, is dry stem branch and the leaf of Verenaceae Callicarpa plant Callicarpa kwangtungensis [Gallicarpa kwangtungensis Chun.].Record in Jiangxi Province's Chinese crude drug standard version in 1996.Be born in the bushes of hillside or the roadside, mountain region, mainly be distributed in ground such as Jiangxi, Guangdong, Hunan, Guangxi.This moral character hardship, puckery, cold has astringing to arrest bleeding, the effect of heat-clearing and toxic substances removing.Be used for spitting of blood, epistaxis is had blood in stool, lung gastrorrhagia, metrorrhagia, cough due to lung-heat, laryngopharynx swelling and pain, burn.Pathopyretic ulcer, traumatic hemorrhage.Callicarpa kwangtungensis Chun is to go all out with the seventies to excavate the medicine of coming in the Chinese herbal medicine mass movement, is used for the treatment of hemorrhage of cervix, gynaecopathias such as vaginitis, cervicitis.Yet up to now, research to Callicarpa kwangtungensis Chun is less, be more common in the report of chemical constituent, roll up the paper that first phase 20-22 pages or leaves are delivered as " Hunan College of Traditional Chinese Medicine's journal " 2005 the 25th: report in " Callicarpa kwangtungensis Chun is position chemical constitution study (II) on the ground ", Chen Yanhua etc. obtain 13 chemical compounds from the separation of Callicarpa kwangtungensis Chun aerial parts, 5 flavone: 5,4-dihydroxy-3,7,3 ' one trimethoxy flavone (5,4-dihydroxy-3,7,3 '-trimethoxyflavone), rhamnazin (rhamnatin), kumatakenin (kumatakenin), Yue Hua element (ermanine), velutin; 3 triterpenes: oleanolic acid (oleanolic acid), ursolic acid (ursolic acid), Betula platyphylla Suk. acid (betulinicacid); 3 phenolic acid: salicylic acid (salicylic acid), syringic acid (syringic acid), isovanillic acid (isovanillic acid); 2 sterols: daucosterol (daucosterol), cupreol (β-sitosterol)
[3]Separation such as Zhou Baiting obtain 10 chemical compound cupreols (β-sitosterol, oleanolic acid (oleanolic acid), ursolic acid (ursolic acid), salicylic acid (salicylic acid), alanine (alamine), succinic acid (succinicacid)
[2]Quercetin (quercetin), Quercetin-3-0-β-D-glucose-alpha-L-rhamnoside (quercetin-3-0-β-D-glucopyranosyl-α-L-rhamnopyranoside), gallic acid (s～lic acid), Folium Callicarpae Formosanae terpene ketone (calliterpenone).
Summary of the invention
The objective of the invention is to: systematically study Callicarpa kwangtungensis Chun hemostatic active component, seek and obtain hemostatic effective site, the preparation method of Callicarpa kwangtungensis Chun extract is provided and is used for the new purposes of hemostatic.
The preparation method of Callicarpa kwangtungensis Chun extract of the present invention is: herb, root, stem and leaf or fruit with Callicarpa kwangtungensis Chun are raw material, adopt a kind of or more than one technologies in solvent extraction method, solvent extraction, resin adsorption method, polyamide adsorpting column chromatography method, normal phase column chromatography, the reversed-phase column chromatography technology, and drying is prepared into Callicarpa kwangtungensis Chun extract.
Preparation method of the present invention comprises also that the Callicarpa kwangtungensis Chun extract that will make combines according to conventional method with excipient and makes powder, granule, tablet, capsule, pill, suppository, enteric agents, injection, syrup, Emulsion, suspensoid, tincture, unguentum or spray.
When the present invention makes different dosage form, can add more than one of lubricant, excipient or binding agent.Described excipient is more than one of starch, lactose, sucrose, mannose, hydroxy methocel.Described lubricant is more than one of fatty acid magnesium, sodium laurylsulfate, Pulvis Talci.Described binding agent dextrin, crystalline cellulose, polyvinylpyrrolidone, more than one of arabic gum, corn starch, gelatin.
The invention provides Callicarpa kwangtungensis Chun extract and be used for the new purposes of hemostatic.
The present invention is simple for process, and the extract hemostatic healing efficacy is better, and has no side effect.
The specific embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1: solvent extraction method/solvent extraction share and extracts the preparation Callicarpa kwangtungensis Chun extract
Callicarpa kwangtungensis Chun 2kg with 80% ethanol 90 ℃ of following reflux three times, merge extractive liquid,, concentrate ethanol extraction.Ethanol extraction is suspended in the water, and with ethyl acetate, n-butanol extraction, n-butanol extracting liquid promptly obtains Callicarpa kwangtungensis Chun extract through concentrate drying, yield 7.1%.
Embodiment 2
Measure clotting time with capillary tube method, the result shows that only gavaging, the Callicarpa kwangtungensis Chun extract more than 1g/kg has tangible haemostatic effect to mice.
Embodiment 3
Callicarpa kwangtungensis Chun extract 50g mixes with microcrystalline Cellulose 45g and magnesium stearate 5g, and granulating mixture is pressed into tablet, every heavy 0.3g of this product, and this film contains Callicarpa kwangtungensis Chun 0.15g.In conjunction with symptom, each 1～2, take every day 2～3 times.
Embodiment 4
Callicarpa kwangtungensis Chun extract 10g and starch 40g add 50% ethanol and make soft material, cross 20 mesh sieves and granulate, the dry granule that gets.In this granule, contain Callicarpa kwangtungensis Chun extract 100mg among every 0.5g.In conjunction with symptom, every day, 0.5～1g took 2～3 every day.
Embodiment 5
Callicarpa kwangtungensis Chun extract 75g mixes with lactose 70g, magnesium stearate 5g, incapsulates.In this capsule, every contains Callicarpa kwangtungensis Chun extract 150mg.In conjunction with symptom, each 1～2, every day 2～3 times.
Embodiment 6
Callicarpa kwangtungensis Chun extract 30g adds water 1000ml and makes dissolving, adds an amount of antiseptic and correctives, sterilization, promptly.The every 1ml of this mixture contains Callicarpa kwangtungensis Chun extract 30mg.In conjunction with symptom, each 5～10ml takes 2～3 every day.
Embodiment 7
Callicarpa kwangtungensis Chun extract 10g is with normal injection agent autofrettage, with the water for injection 1000ml that is heated to 60 ℃, opens with sodium chloride adjusting etc., encloses the peace bottle.Contain Callicarpa kwangtungensis Chun extract 100mg among the every 10ml of this injection.In conjunction with symptom, each intramuscular injection 10ml injects 2～3 every day.
Claims (8)
1, a kind of preparation method of Callicarpa kwangtungensis Chun extract, it is characterized in that: herb, root, stem and leaf or fruit with Callicarpa kwangtungensis Chun are raw material, adopt a kind of or more than one technologies in solvent extraction method, solvent extraction, resin adsorption method, polyamide adsorpting column chromatography method, normal phase column chromatography, the reversed-phase column chromatography technology, and drying is prepared into Callicarpa kwangtungensis Chun extract.
2, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 1 is characterized in that: the Callicarpa kwangtungensis Chun extract of making is combined according to conventional method with excipient make powder, granule, tablet, capsule, pill, suppository, enteric agents, injection, syrup, Emulsion, suspensoid, tincture, unguentum or spray.
3, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 1 and 2 is characterized in that: add lubricant.
4, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 2 is characterized in that: excipient is more than one of starch, lactose, sucrose, mannose, hydroxy methocel.
5, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 3 is characterized in that: lubricant is more than one of fatty acid magnesium, sodium laurylsulfate, Pulvis Talci.
6, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 1 and 2 is characterized in that: add binding agent.
7, the preparation method of Callicarpa kwangtungensis Chun extract according to claim 6 is characterized in that: binding agent dextrin, crystalline cellulose, polyvinylpyrrolidone, more than one of arabic gum, corn starch, gelatin.
8, the new purposes of a kind of Callicarpa kwangtungensis Chun extract is used for hemostasis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101364048A CN101417044A (en) | 2008-12-09 | 2008-12-09 | Preparation method Kwangtung purple beautyberry extract and new use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101364048A CN101417044A (en) | 2008-12-09 | 2008-12-09 | Preparation method Kwangtung purple beautyberry extract and new use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101417044A true CN101417044A (en) | 2009-04-29 |
Family
ID=40628170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101364048A Pending CN101417044A (en) | 2008-12-09 | 2008-12-09 | Preparation method Kwangtung purple beautyberry extract and new use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101417044A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040635A (en) * | 2010-11-23 | 2011-05-04 | 成都普思生物科技有限公司 | Method for efficiently separating and purifying forsythiaside B monomer and poliumoside monomer |
| CN102266437A (en) * | 2011-07-21 | 2011-12-07 | 应群芳 | Vacuum freeze-drying process of callicarpa leaf and preparation process of callicarpa leaf powder |
| CN111423481A (en) * | 2020-04-13 | 2020-07-17 | 中国科学院西北高原生物研究所 | Label of Lepidium europaeum and preparation method and application thereof |
| CN112402687A (en) * | 2020-11-26 | 2021-02-26 | 嘉应学院 | Callicarpa kwangtungensis liquid band-aid and preparation method thereof |
-
2008
- 2008-12-09 CN CNA2008101364048A patent/CN101417044A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040635A (en) * | 2010-11-23 | 2011-05-04 | 成都普思生物科技有限公司 | Method for efficiently separating and purifying forsythiaside B monomer and poliumoside monomer |
| CN102266437A (en) * | 2011-07-21 | 2011-12-07 | 应群芳 | Vacuum freeze-drying process of callicarpa leaf and preparation process of callicarpa leaf powder |
| CN102266437B (en) * | 2011-07-21 | 2013-03-13 | 杭州求本植物科技有限公司 | Vacuum freeze-drying process of callicarpa leaf and preparation process of callicarpa leaf powder |
| CN111423481A (en) * | 2020-04-13 | 2020-07-17 | 中国科学院西北高原生物研究所 | Label of Lepidium europaeum and preparation method and application thereof |
| CN111423481B (en) * | 2020-04-13 | 2021-06-22 | 中国科学院西北高原生物研究所 | Label of Lepidium europaeum and preparation method and application thereof |
| CN112402687A (en) * | 2020-11-26 | 2021-02-26 | 嘉应学院 | Callicarpa kwangtungensis liquid band-aid and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) FH Chen: a review | |
| CN102205000B (en) | Callicarpa nudiflora Hook. et Arn. extract, preparation method and application thereof | |
| WO2018058261A1 (en) | Traditional chinese medicine composition for treating psoriasis and preparation method thereof | |
| Cappelletti et al. | External antirheumatic and antineuralgic herbal remedies in the traditional medicine of north-eastern Italy | |
| CN101417044A (en) | Preparation method Kwangtung purple beautyberry extract and new use thereof | |
| CN107929544B (en) | Preparation method and application of mileanine part and monomer in bletilla plants | |
| CN105982970B (en) | A Chinese medicinal composition for treating psoriasis, and its preparation method | |
| CN101234159A (en) | Pharmaceutical combination with synergistic reaction | |
| CN107582899B (en) | Mongolian medicine for clearing heat and stopping bleeding and its preparing process | |
| CN103705725B (en) | Chinese medicine composition of a kind of moistening and cleaning throat antiinflammatory and preparation method thereof | |
| CN103751717B (en) | A kind of pharmaceutical composition for the treatment of hemorrhoid | |
| CN104892419B (en) | A kind of total coffee acid ester extract of multiradiate fleabane | |
| KR101210405B1 (en) | Radix salviae miltiorrhizaeextract and composition thereof for the treatment of the aspirin resistance diseases | |
| CN102488745A (en) | Extract of overground part of gentiana scabra bge. and its preparation method and application | |
| CN108743657A (en) | The preparation method of methods of glycosides in a kind of mussot swertia herb | |
| CN107875157A (en) | The application of hederagenin and its glucosides in the medicine for preparing preventing and treating cerebral apoplexy | |
| CN103356731B (en) | Pharmaceutical composition containing Cortex Eucommiae extract and Radix Notoginseng total arasaponins and application thereof | |
| CN103349724A (en) | Anemarrhenae rhizoma total flavone extract and method for concurrently preparing anemarrhenae rhizoma total flavone extract and anemarrhenae rhizoma total saponin extract | |
| CN102614253B (en) | Composition for nourishing and protecting liver and application of composition | |
| CN101693073A (en) | Compound caesalpinia pulcherrima spraying agent and preparation method thereof | |
| CN102125590B (en) | Ginsenoside Rg1-containing pseudo-ginseng active ingredient as well as preparation method and application thereof | |
| CN101269123A (en) | Secondary development novel technique for thirst eliminating capsule for lowering blood sugar | |
| CN104547026A (en) | Preparation method and application of salvia miltiorrhiza leave and panax pseudo-ginseng extract | |
| CN108743800A (en) | A kind of traditional Chinese medicine powder for treating cardiovascular and cerebrovascular | |
| CN101810335B (en) | Method for preparing health-care foods with different activities by comprehensively using dogwoods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090429 |