CN101416608A - Test-tube breeding cultivation method of floral leaf Bambusa edulis - Google Patents
Test-tube breeding cultivation method of floral leaf Bambusa edulis Download PDFInfo
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- CN101416608A CN101416608A CNA200810121942XA CN200810121942A CN101416608A CN 101416608 A CN101416608 A CN 101416608A CN A200810121942X A CNA200810121942X A CN A200810121942XA CN 200810121942 A CN200810121942 A CN 200810121942A CN 101416608 A CN101416608 A CN 101416608A
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- floral leaf
- edulis
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- bambusa
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to vitro breeding and reproduction of mosaic bambusa edulis, comprising steps of: a, choosing those vitro mosaic plantlets which have been through stable idiovariation as explants to subculture; b, root induction and transplanting, namely transplanting the plantlets to culture medium which is used for root induction and culturing for 60 days so as to reach 100% of rooting rate in normal, moving the plantlets to domestication room for two weeks and then transplanting in man-made substrate, planting the plantlets in greenhouse after one week. The invention propagates the species which is rare to find and stable in natural mutation and hereditary character, and it also cultivates novel species of mosaic bambusa edulis with good afforestation value, edible value and ornamental value.
Description
[technical field]
The present invention relates to a kind of method of carrying out test tube breeding and propagation bamboo new varieties by tissue culture technique.
[background technology]
Crow pin green bamboo (Bambosa edulis) is a kind of medium-sized bamboo that grows thickly that grass family Bambusoideae green bamboo belongs to, and higher greening, ornamental value are arranged, and especially uses bamboo as bamboo shoot, goes out bamboo shoot morning, output height, quality better.The pole of crow pin green bamboo is upright, is light green, and up to 20 meters, base portion is thicker.The every joint of pole is given birth to racemosus, is pencil and grows thickly, leaf green.
Chancing in the black pin green bamboo seedling that test tube is bred as far back as April 19 nineteen ninety has a strain bud mutation seedling, its branch base portion 3-4 sheet blade adularescent striped, select and remain at once, the floral leaf seedling of this bud mutation as cultivating body, is carried out the test tube subculture and expands numerous and further transplant potted plant.So far 18 years floral leaf proterties surplus, that its spontaneous mutation forms all can genetic stability.Now with this floral leaf Hsinchu, called after floral leaf crow pin green bamboo temporarily.
[summary of the invention]
The technical problem to be solved in the present invention is the rare seedling that this spontaneous mutation forms to be used the method for test tube breeding and propagation, enlarges population as soon as possible, breeds out a kind of black pin green bamboo new varieties that have more ornamental value, increases the newcomer in the bamboo class family.
The scheme that solves the problems of the technologies described above is: this test-tube breeding cultivation method of floral leaf Bambusa edulis process the following step:
(1) subculture of floral leaf sudden change seedling expands numerous: newfound floral leaf sudden change test tube seedling is made to cultivate body, per 6 all subcultures expand numerous, be inoculated in the seedling differential medium, this medium is by MS salt and White ' s vitamin, the BA of the quartzy agar of the inositol of the sucrose of 30g/L, 100mg/L, 2.5g/L, adding 3mg/L is that the NAA of benzyl aminoadenine, 1mg/L is that α-naa is formulated in addition, 25 ℃ of temperature, intensity of illumination 2500lux, light application time 16h/ days, cultivated 45 days, and must rise appreciably and break up new budlet;
(2) root induction and transplanting: when budlet grows to the 3-5cm height, the BA that is inoculated in the step (1) is reduced to 0mg/L, cultivated 60 days in the identical medium of other composition and content, rooting rate reaches 100% under the normal condition, can be moved to the domestication chamber, transplant down by two weeks of domestication for high light 20000lux, elder generation's flush away root medium, moving in three's volume ratio is vermiculite: potted plant in the artificial mixed-matrix of perlite: peat=1:1:1, every basin overlaps a transparent plastic bag, this bag cut an osculum in per 2 days, the back immigration greenhouse plantation of two weeks.
The invention has the beneficial effects as follows the seedling that extremely difficult generation spontaneous mutation serendipitous is again presented floral leaf proterties and energy genetic stability, the seedling that promptly is called floral leaf crow pin green bamboo, breed expanding species in a short time and become a kind of black pin green bamboo new varieties, keeping good greening, bamboo shoot, further improve the ornamental value of this bamboo again with when being worth.
[embodiment]
The present invention is described in further detail below in conjunction with embodiment: in the medium that this floral leaf crow pin green bamboo test tube is bred, the auxin of selecting for use is the NAA that 3mg/LBA adds 1mg/L, this be through BA with 0,0.3,1,3,10mg/L adds five kinds of concentration repetitions of NAA 3 times of 1mg/L, each every kind of concentration has the comparative trial of 20 cultivation bodies to draw, the NAA that the BA of 3mg/L adds 1mg/L handled after 75 days, and it is maximum that budlet increases the fastest differentiation.In a word, medium disclosed in this invention and proportioning value thereof, condition of culture such as temperature, intensity of illumination is preferred version, substitute such as glucose, agar etc. as carbohydrate nutrition base, coagulating agent etc. are also available, each proportion of raw materials value of medium also can adopt replacing near value of preferred plan definite value of the present invention respectively, and this is conspicuous.Provide most preferred embodiment of the present invention below.
This test-tube breeding cultivation method of floral leaf Bambusa edulis process the following step:
(1) subculture of floral leaf sudden change seedling expands numerous: newfound floral leaf sudden change test tube seedling is made to cultivate body, per 6 all subcultures expand numerous, be inoculated in the seedling differential medium, this medium is by MS salt and White ' s vitamin, the BA of the quartzy agar of the inositol of the sucrose of 30g/L, 100mg/L, 2.5g/L, adding 3mg/L is that the NAA of benzyl aminoadenine, 1mg/L is that α-naa is formulated in addition, 25 ℃ of temperature, intensity of illumination 2500lux, light application time 16h/ days, cultivated 45 days, and must rise appreciably and break up new budlet;
(2) root induction and transplanting: when budlet grows to the 3-5cm height, the BA that is inoculated in the step (1) is reduced to 0mg/L, cultivated 60 days in the identical medium of other composition and content, rooting rate reaches 100% under the normal condition, can be moved to the domestication chamber, transplant down by two weeks of domestication for high light 20000lux, elder generation's flush away root medium, moving in three's volume ratio is vermiculite: potted plant in the artificial mixed-matrix of perlite: peat=1:1:1, every basin overlaps a transparent plastic bag, this bag cut an osculum in per 2 days, the back immigration greenhouse plantation of two weeks.
MS salt and White ' s vitamin are commercially available in the above-mentioned medium, press the catalogue adapted, and is known for going together.
Claims (1)
1, a kind of test-tube breeding cultivation method of floral leaf Bambusa edulis is characterized in that through the following step:
(1) subculture of floral leaf sudden change seedling expands numerous: newfound floral leaf sudden change test tube seedling is made to cultivate body, per 6 all subcultures expand numerous being inoculated in the seedling differential medium, this medium is by MS salt and White ' s vitamin, the BA of the quartzy agar of the inositol of the sucrose of 30g/L, 100mg/L, 2.5g/L, adding 3mg/L is that the NAA of benzyl aminoadenine, 1mg/L is that α-naa is formulated in addition, 25 ℃ of temperature, intensity of illumination 25001ux, light application time 16h/ days, cultivated 45 days, and must rise appreciably and break up new budlet;
(2) root induction and transplanting: when budlet grows to the 3-5cm height, the BA that is inoculated in the step (1) is reduced to 0mg/L, cultivated 60 days in the identical medium of other composition and content, rooting rate reaches 100% under the normal condition, can be moved to the domestication chamber, transplant down by two weeks of domestication for high light 200001ux, elder generation's flush away root medium, moving in three's volume ratio is vermiculite: potted plant in the artificial mixed-matrix of perlite: peat=1:1:1, every basin overlaps a transparent plastic bag, this bag cut an osculum in per 2 days, the back immigration greenhouse plantation of two weeks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810121942XA CN101416608A (en) | 2008-10-23 | 2008-10-23 | Test-tube breeding cultivation method of floral leaf Bambusa edulis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810121942XA CN101416608A (en) | 2008-10-23 | 2008-10-23 | Test-tube breeding cultivation method of floral leaf Bambusa edulis |
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| CN101416608A true CN101416608A (en) | 2009-04-29 |
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| CNA200810121942XA Pending CN101416608A (en) | 2008-10-23 | 2008-10-23 | Test-tube breeding cultivation method of floral leaf Bambusa edulis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907535A (en) * | 2014-04-16 | 2014-07-09 | 南京林业大学 | Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture |
| CN105028211A (en) * | 2015-08-28 | 2015-11-11 | 浙江农林大学 | Establishment method of rapid and efficient regeneration system by adopting sinocalamus oldhami flower shoots |
-
2008
- 2008-10-23 CN CNA200810121942XA patent/CN101416608A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907535A (en) * | 2014-04-16 | 2014-07-09 | 南京林业大学 | Method for obtaining large number of bambusa glaucophylla regeneration plants through tissue culture |
| CN105028211A (en) * | 2015-08-28 | 2015-11-11 | 浙江农林大学 | Establishment method of rapid and efficient regeneration system by adopting sinocalamus oldhami flower shoots |
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Open date: 20090429 |